Portable back-illuminated optical imaging microscope

Abstract

The present invention relates to the technical field of microscopes, and discloses a portable back-illuminated optical imaging microscope, including an objective lens, a Z-direction displacement control module, an XY-direction displacement control module, a back-illuminated module, an imaging unit adapter module, an imaging unit, and an eyepiece; the portable back-illuminated optical imaging microscope provided by the present invention avoids the traditional Kohler illumination light source structure, and the light source does not need to use the secondary imaging image as the light source to illuminate the sample, but directly uses an LED with a surface light source characteristic as the light source and uses an axial displacement control module for direct back-illuminated illumination. In this way, the distance originally required to be introduced by the Kohler illumination optical path structure is shortened, so that the structure and volume of the microscope are further reduced. Therefore, this design uses a planar LED light source as the lighting system, which greatly reduces the cost and occupied space of the overall system.

Description

Technical Field

[0001] The invention relates to the technical field of microscopes, in particular to a portable back-illuminated optical imaging microscope. Background Art

[0002] Ordinary optical imaging microscope refers to an optical imaging microscope that uses traditional visible light for illumination. The light source is different from that of fluorescence imaging microscope and nonlinear imaging microscope. The imaging illumination method and use of ordinary optical imaging microscope are introduced as follows:

[0003] There are two main types of illumination: forward transmission illumination (the illuminating light penetrates the sample and enters the objective lens), and back-illumination reflection illumination (the illuminating light passes through the objective lens to the sample, and then returns to the objective lens after being reflected by the sample) in optical microscopes.

[0004] Uses and their corresponding technical structures and processes:

[0005] The technologies used in both illumination methods are based on the principle of Köhler illumination. The so-called Köhler illumination uses the image of the filament as a secondary light source to illuminate the sample. The purpose is to eliminate the uneven illumination caused by using the filament to directly image the sample for illumination. The basic principle of secondary illumination is the principle of sub-wave superposition, that is, each point on the filament image plane is used as a point light source for secondary light emission, and illumination is performed according to the principle of sub-wave superposition. The advantage of this is that the illumination at the sample is basically uniform.

[0006] Forward-illumination transmission-type microscopes are mainly biological microscopes, and back-illumination reflection-type microscopes are mainly metallographic microscopes.

[0007] Problems and shortcomings of the two lighting methods:

[0008] Microscopes using Köhler illumination are relatively large, relatively expensive, and not suitable for portable single-body operation. Although there are also cheap transmission optical microscopes that use LED as light source, they are slightly larger, not convenient for carrying, and are easily affected by the transparency of the sample. In other words, the existing cheaper optical imaging microscopes usually use forward transmission illumination and use transparent sample preparation to make samples for imaging. For special opaque samples, this imaging microscope is no longer suitable.

[0009] The length of the lens barrel and the way to adjust the displacement of the sample make the microscope not portable and appear very bulky. At the same time, the magnification of the microscope will be limited by the small magnification of the objective lens and eyepiece used. The optical imaging microscope is an imaging organism composed of an objective lens and an illumination light source, so it must satisfy the law of optical imaging. The traditional biological optical imaging microscope mainly uses transmission illumination, resulting in the observed samples being basically transparent preparations; although the metallographic microscope uses back-illumination imaging, the illumination light source used is a halogen lamp or a halogen tungsten lamp. This illumination method is affected by the traditional Kohler illumination imaging optical principle (the image of the illumination light source filament is used as the light source to illuminate the sample), resulting in the imaging microscope being larger in size and more expensive. Summary of the invention

[0010] The purpose of the present invention is to provide a portable back-illuminated optical imaging microscope, aiming to solve the problem that the microscope in the prior art has a single function and is inconvenient to carry.

[0011] The present invention is implemented in this way: a portable back-illuminated optical imaging microscope comprises an objective lens, a Z-direction displacement control module, an XY-direction displacement control module, a back-illuminated module, an imaging unit adapter module, an imaging unit and an eyepiece; the Z-direction displacement control module is used to control the distance between the objective lens and a sample to be observed, and the XY-direction displacement control module is used to control the position of the observation field of the objective lens; the back-illuminated module comprises a semi-planar LED illumination light source, a spectroscopic plate, a mounting fixture for mounting the semi-planar LED illumination light source and the spectroscopic plate, and a displacement groove, and the mounting fixture can be slidably arranged on the The imaging unit is used to receive the optical signal and generate image data according to the received optical signal; the imaging unit adapter module includes a slidable reflector; wherein, the light emitted by the semi-planar LED light source enters the objective lens through the reflection of the beam splitter plate, irradiates the sample to be observed, and the sample to be observed reflects the light back to the objective lens, and then transmits the beam splitter plate to form a first light ray, when the reflector slides to the position opposite to the first light ray, the first light ray is reflected by the reflector to the imaging unit for imaging; when the reflector slides to other positions, the first light ray directly enters the eyepiece for human eye observation.

[0012] Furthermore, the imaging unit adapter module also includes an adapter, which includes a horizontally arranged first light channel and a vertically arranged second light channel, the middle of the first light channel is connected to the middle of the second light channel, the first light channel is arranged opposite to the eyepiece, and the second light channel is arranged opposite to the imaging unit; the adapter has a slide groove, and the reflector can be slidably set in the slide groove.

[0013] Furthermore, the XY direction displacement control module includes a screw rod along the X-axis direction and a screw rod along the Y-axis direction, the X-axis direction screw rod is arranged along the X-axis direction, the X-axis direction screw rod is used to abut the objective lens to realize the X-direction displacement of the objective lens, the Y-axis direction screw rod is arranged along the Y-axis direction, and the Y-axis direction screw rod is used to abut the objective lens to realize the Y-direction displacement of the objective lens.

[0014] Furthermore, the Z-direction displacement control module includes a screw rod arranged along the Z-axis direction, the screw rod is connected to the objective lens, and when the screw rod is rotated, the objective lens moves along the Z-axis direction.

[0015] Furthermore, the imaging unit includes an imaging sensor, an AD converter, a single-chip microcomputer, and a display, wherein: the imaging sensor is used to convert an optical signal into an electrical signal and transmit it to the AD converter; the AD converter is used to process the electrical signal sent by the imaging sensor, and convert the electrical signal into a digital signal and send it to the single-chip microcomputer; the single-chip microcomputer is used to encode the digital signal sent by the AD converter to obtain image data.

[0016] Furthermore, the imaging sensor is a CCD sensor.

[0017] Furthermore, the imaging sensor is a CMOS sensor.

[0018] Furthermore, the portable back-illuminated optical imaging microscope includes a communication module for establishing a communication connection with a terminal, and the communication module is connected to the single-chip microcomputer.

[0019] Furthermore, a shock-absorbing pad is provided on the outer periphery of the objective lens.

[0020] Compared with the prior art, the portable back-illuminated optical imaging microscope provided by the present invention avoids the traditional Kohler illumination light source structure. The light source does not need to use the secondary imaging image as the light source to illuminate the sample, but directly uses an LED with a surface light source characteristic as the light source for direct back-illuminated illumination. This shortens the distance that the Kohler illumination optical path structure originally needed to introduce, and further reduces the structure of the microscope. Therefore, this design uses a planar LED light source as the lighting system, greatly reducing the cost and space occupied by the overall system. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a three-dimensional schematic diagram of a portable back-illuminated optical imaging microscope provided by an embodiment of the present invention;

[0022] Figure 2 is an exploded schematic diagram of a portable back-illuminated optical imaging microscope provided by an embodiment of the present invention;

[0023] Figure 3is an exploded schematic diagram of an imaging unit adapter module and an imaging unit provided in an embodiment of the present invention;

[0024] Figure 4 It is a schematic diagram of an exploded view of a backlighting module provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0026] The same or similar numbers in the drawings of this embodiment correspond to the same or similar parts; in the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the drawings, it is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and cannot be understood as limitations on this patent. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to specific circumstances.

[0027] The implementation of the present invention is described in detail below in conjunction with specific embodiments.

[0028] Reference Figure 1-4 As shown, a preferred embodiment of the present invention is provided.

[0029] The portable back-illuminated optical imaging microscope comprises an objective lens 1, a Z-direction displacement control module, an XY-direction displacement control module 2, a back-illuminated module 5, an imaging unit adapter module 6, an imaging unit 3 and an eyepiece 4; the Z-direction displacement control module is used to control the distance between the objective lens 1 and a sample to be observed, and the XY-direction displacement control module 2 is used to control the position of the observation field of the objective lens 1; the back-illuminated module 5 comprises a semi-planar LED illumination light source 51, a beam splitter plate 52, a mounting fixture 53 for mounting the semi-planar LED illumination light source 51 and the beam splitter plate 52, and a displacement groove 54, wherein the mounting fixture 53 can be slidably arranged Placed in the displacement slot 54; the imaging unit 3 is used to receive the light signal and generate image data according to the received light signal; the imaging unit adapter module 6 includes a slidable reflector 62; wherein, the light emitted by the semi-planar LED light source enters the objective lens 1 after being reflected by the beam splitter plate 52, irradiates the sample to be observed, and the sample to be observed reflects the light back to the objective lens 1, and then transmits through the beam splitter plate 52 to form a first light. When the reflector 62 slides to the opposite position of the first light, the first light is reflected by the reflector 62 to the imaging unit 3 for imaging; when the reflector 62 slides to other positions, the first light directly enters the eyepiece 4 for human eye observation.

[0030] The imaging principle of the portable back-illuminated optical imaging microscope provided above is: the light emitted by the semi-planar LED light source enters the objective lens 1 after being reflected by the beam splitter plate 52, irradiates the sample to be observed, and the sample to be observed reflects the light back to the objective lens 1, and then transmits through the beam splitter plate 52 to form a first light ray. When the reflector 62 is slid to a position relative to the first light ray, the first light ray is reflected by the reflector 62 to the imaging unit 3 for imaging. When the reflector 62 slides to other positions, the first light ray directly enters the eyepiece 4 for observation by the human eye.

[0031] Traditional biological optical imaging microscopes mainly use transmission illumination, which means that the samples observed are basically transparent preparations; although metallographic microscopes use back-illuminated imaging, the illumination source used is a halogen lamp or a halogen tungsten lamp, which is affected by the traditional Kohler illumination imaging optical principle (the image of the illumination light source filament is used as the light source to illuminate the sample). As a result, the imaging microscope is larger in size and more expensive.

[0032] The present invention is aimed at the drawbacks of the conventional microscope due to the Kohler illumination structure, and invents the portable back-illuminated optical imaging microscope. The invention is creatively designed to solve the contradictions between displacement, portable size and observation imaging stability.

[0033] The portable back-illuminated optical imaging microscope provided by the present invention avoids the traditional Kohler illumination light source structure. The light source does not need to use the secondary imaging image as the light source to illuminate the sample, but directly uses an LED with a surface light source characteristic as the light source for direct back-illuminated illumination. This shortens the distance that the Kohler illumination optical path structure originally needed to introduce, and further reduces the structure of the microscope. Therefore, this design uses a planar LED light source as the illumination system, greatly reducing the cost and space occupied by the overall system.

[0034] Explanation of the imaging principle of this imaging system: When a uniform light source acts on an object, the secondary diffuse light on the surface of the object is imaged through a lens group, and the imaging principle also satisfies the Gaussian imaging principle of geometric optics.

[0035] The core technology of the present invention is mainly reflected in the lighting method, which breaks through the limitation of the light source of the traditional optical imaging microscope by the Kohler illumination imaging system. The relatively cheap facet-type white light LED is used as the light source, making the core part cheaper, so as to improve the convenience of the portable microscope in field operations.

[0036] Specifically, the imaging unit adapter module 6 also includes an adapter 61, which includes a horizontally arranged first light channel 612 and a vertically arranged second light channel 613. The middle of the first light channel 612 is connected to the middle of the second light channel 613. The first light channel 612 is arranged opposite to the eyepiece 4, and the second light channel 613 is arranged opposite to the imaging unit 3; the adapter 61 has a slide groove 611, and the reflector 62 can be slidably arranged in the slide groove 611; in this way, when the reflector 62 is slid to the opposite position of the first light, the first light is reflected by the reflector 62 to the imaging unit 3 for imaging; when the reflector 62 is slid to other positions, the first light directly enters the eyepiece 4 for observation by the human eye.

[0037] Specifically, the XY direction displacement control module 2 includes an X-axis direction screw 21 and a Y-axis direction screw 22, the X-axis direction screw 21 is arranged along the X-axis direction, and the X-axis direction screw 21 is used to abut the objective lens 1, and the Y-axis direction screw 22 is arranged along the Y-axis direction, and the Y-axis direction screw 22 is used to abut the objective lens 1; in this way, by screwing the X-axis direction screw 21, the objective lens 1 can be controlled to move along the X-axis direction, and by screwing the Y-axis direction screw 22, the objective lens 1 can be controlled to move along the Y-axis direction. The movement of the objective lens 1 along the X-axis direction and the Y-axis direction can be realized through the XY direction control module, thereby realizing the control of the position of the observation field of view of the objective lens 1.

[0038] Furthermore, the Z-direction displacement control module includes a screw arranged along the Z-axis direction, the screw is threadedly connected to the objective lens 1, and when the screw is rotated, the objective lens 1 moves along the Z-axis direction, thereby achieving control of the distance between the objective lens 1 and the sample to be observed.

[0039] In this embodiment, the imaging unit 3 includes an imaging sensor, an AD converter, a single-chip microcomputer, and a display, wherein: the imaging sensor is used to convert an optical signal into an electrical signal and transmit it to the AD converter; the AD converter is used to process the electrical signal sent by the imaging sensor, and convert the electrical signal into a digital signal and send it to the single-chip microcomputer; the single-chip microcomputer is used to encode the digital signal sent by the AD converter to obtain image data.

[0040] In this embodiment, the imaging sensor is a CCD sensor.

[0041] Alternatively, as another embodiment, the imaging sensor is a CMOS sensor.

[0042] In this embodiment, the portable back-illuminated optical imaging microscope includes a communication module for establishing a communication connection with a terminal, and the communication module is connected to the single-chip microcomputer; in this way, a communication connection is established between the communication module and the terminal, so that the image data encoded by the single-chip microcomputer is transmitted to the terminal, and the terminal stores and displays the image data.

[0043] Furthermore, a shock-absorbing pad is provided on the outer ring of the objective lens 1. The arrangement of the shock-absorbing pad breaks through the limitation of the practical environment, so that the portable back-illuminated optical imaging microscope can be applied to a wider range of usage scenarios and environments.

[0044] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A portable back-illuminated optical imaging microscope, characterized in that: The invention comprises an objective lens, a Z-direction displacement control module, an XY-direction displacement control module, a backlighting module, an imaging unit adapter module, an imaging unit and an eyepiece; the Z-direction displacement control module is used to control the distance between the objective lens and the sample to be observed, the XY-direction displacement control module is used to control the position of the observation field of the objective lens, the backlighting module comprises a semi-planar LED lighting source, a beam splitter plate, a mounting fixture for mounting the semi-planar LED lighting source and the beam splitter plate, and a displacement groove, wherein the mounting fixture can be slidably arranged in the displacement groove; the imaging unit is used to The imaging unit adapter module receives a light signal and generates image data according to the received light signal; the imaging unit adapter module includes a slidable reflector; wherein the light emitted by the semi-planar LED illumination light source enters the objective lens through reflection by the beam splitter plate, irradiates the sample to be observed, and the sample to be observed reflects the light back to the objective lens, and then transmits the beam splitter plate to form a first light ray; when the reflector slides to a position opposite to the first light ray, the first light ray is reflected by the reflector to the imaging unit for imaging; when the reflector slides to other positions, the first light ray directly enters the eyepiece for human eye observation.

2. The portable back-illuminated optical imaging microscope according to claim 1, characterized in that: The imaging unit adapter module also includes an adapter, which includes a horizontally arranged first light channel and a vertically arranged second light channel, the middle of the first light channel is connected to the middle of the second light channel, the first light channel is arranged opposite to the eyepiece, and the second light channel is arranged opposite to the imaging unit; the adapter has a slide groove, and the reflector can be slidably set in the slide groove.

3. The portable back-illuminated optical imaging microscope according to claim 1, characterized in that: The XY direction displacement control module includes a screw rod along the X-axis direction and a screw rod along the Y-axis direction. The X-axis direction screw rod is arranged along the X-axis direction and is used to abut the objective lens to achieve the X-direction displacement of the objective lens. The Y-axis direction screw rod is arranged along the Y-axis direction and is used to abut the objective lens to achieve the Y-direction displacement of the objective lens.

4. The portable back-illuminated optical imaging microscope according to claim 1, characterized in that: The Z-direction displacement control module includes a screw rod arranged along the 2-axis direction, the screw rod is threadedly connected with the objective lens, and when the screw rod is rotated, the objective lens moves along the Z-axis direction.

5. The portable back-illuminated optical imaging microscope according to any one of claims 1 to 4, characterized in that: The imaging unit includes an imaging sensor, an AD converter, a single-chip microcomputer, and a display, wherein the imaging sensor is used to convert an optical signal into an electrical signal and transmit the electrical signal to the AD converter; the AD converter is used to process the electrical signal sent by the imaging sensor, and convert the electrical signal into a digital signal and send it to the single-chip microcomputer; the single-chip microcomputer is used to encode the digital signal sent by the AD converter to obtain image data.

6. The portable back-illuminated optical imaging microscope according to claim 5, characterized in that: The imaging sensor is a CCD sensor.

7. The portable back-illuminated optical imaging microscope according to claim 5, characterized in that: The imaging sensor is a CMOS sensor.

8. The portable back-illuminated optical imaging microscope according to claim 5, characterized in that: The portable back-illuminated optical imaging microscope comprises a communication module for establishing a communication connection with a terminal, and the communication module is connected to the single-chip microcomputer.

9. The portable back-illuminated optical imaging microscope according to claim 5, characterized in that: A shock-absorbing pad is arranged on the outer peripheral ring of the objective lens.

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