A cytology composite effect simulation system based on automatic control

Abstract

The present invention relates to a cytological composite effect simulation system based on automatic control, comprising a sub-magnetic experiment component, a control component, a low-gravity experiment component, a housing, a cell culture environment control component, and a control system; the sub-magnetic experiment component, the control component, and the low-gravity experiment component are arranged inside the housing; the sub-magnetic experiment component is used to establish a sub-magnetic environment meeting the experimental requirements; the control component is used to establish an experimental environment; the low-gravity experiment component is used to drive the sub-magnetic experiment component to rotate according to the experimental requirements to simulate the low-gravity environment required by the experiment; the cell culture environment control component is used to establish the temperature and CO2 concentration conditions required for cell experiments in the cell experiment area; the control system is used to regulate the cell culture environment control component and the low-gravity experiment component to work according to the experimental requirements. The present invention can establish different levels of low-gravity and sub-magnetic composite environments and realize the automatic control of the cell culture environment, supporting the research on cytological effects of space low-gravity and sub-magnetic composite environments on the ground.

Description

Technical Field

[0001] The present invention belongs to the technical field of cell biology, and particularly relates to a cytological composite effect simulation system based on automatic control. Background Art

[0002] With the continuous deepening of people's space exploration, the cytological effects of the space environment, especially the research on the cytological composite effects of the space environment, have attracted more and more attention. However, due to the limited opportunities for real-time space flight experiments and extremely limited experimental resource conditions, carrying out relevant space composite effect simulation research on the ground has become a necessary way for related disciplines to explore the mysteries of space and accumulate space knowledge.

[0003] Carrying out research on cytological composite effects related to the space environment on the ground requires necessary simulation equipment as support. However, there is currently no cytological effect research platform with perfect functions, especially without the ability to establish different levels of low gravity and sub-magnetic composite environments.

[0004] Currently, a series of rotators (RotateWall Vessels, RWVs) produced in the United States are often used to carry out simulation research on space microgravity effects, while shielding boxes are used to carry out sub-magnetic cytological effect research. The technical defects of using these independent and unrelated devices to carry out space composite effect research include:

[0005] 1) The above-mentioned existing devices cannot achieve different levels of low gravity environments required for relevant space environmental medical research.

[0006] 2) Using the experimental mode of the above-mentioned independent devices, it is impossible to effectively synchronize the experiments on the effects of low gravity environment and sub-magnetic environment, which limits the research on cytological composite effects of low gravity and sub-magnetism on the ground.

[0007] 3) The above-mentioned existing devices do not integrate an automatic control system for the cell culture environment and cannot independently maintain the conditions required for cell culture experiments, which restricts the long-term development of relevant experiments. Summary of the Invention

[0008] The purpose of the present invention is to provide a cytological composite effect simulation system based on automatic control, establish different levels of low gravity and sub-magnetic composite environments, and realize the automatic control of the cell culture environment to support the research on cytological effects of space low gravity and sub-magnetic composite environments on the ground.

[0009] The present invention provides a cytological composite effect simulation system based on automatic control, including a sub-magnetic experiment component, a control component, a low gravity experiment component, a housing, a cell culture environment control component, and a control system; the housing is a box structure, and the sub-magnetic experiment component, the control component, and the low gravity experiment component are arranged in the housing to form a closed cell experiment area in the housing.

[0010] The sub-magnetic experimental component is used to establish a sub-magnetic environment with a low magnetic field intensity that meets the experimental requirements in the area where the cell sample is located;

[0011] The control component is used to establish an experimental environment for the control sample group of the experiment;

[0012] The low-gravity experimental component is used to drive the sub-magnetic experimental component to rotate according to the experimental requirements to simulate a low-gravity environment required by the experiment;

[0013] The cell culture environment control component is used to establish temperature and CO2 concentration conditions required for cell experiments in the cell experiment area;

[0014] The control system is used to regulate the cell culture environment control component and the low-gravity experimental component to work according to the experimental requirements.

[0015] Furthermore, the sub-magnetic experimental component includes an end cap, an experimental component sample holder, a cylindrical tubular coil, a magnetic isolation component, a convection fan, and a slip ring; the slip ring and the convection fan are fixed to one end of the magnetic isolation component; the cylindrical tubular coil is installed inside the magnetic isolation component; the experimental component sample holder is installed inside the cylindrical tubular coil through the track inside the cylindrical tubular coil; the end cap is buckled to the other end of the magnetic isolation component.

[0016] Furthermore, the end cap is composed of an inner cover, a magnetic isolation plate, and an outer cover, and the inner cover and the outer cover are respectively bonded to both sides of the magnetic isolation plate;

[0017] The experimental component sample holder is used to install experimental samples and includes a main structure and a buffer member, and the buffer member is bonded to the main structure;

[0018] The cylindrical tubular coil is used to generate a uniform magnetic field required by the experiment and includes a track, a support tube, and an enameled wire spirally wound outside the support tube, and the track is bonded to the inner surface of the support tube;

[0019] The magnetic isolation component is used to isolate the magnetic field in the experimental environment and includes a magnetic isolation layer, a protective outer layer, a protective inner layer, and a transmission block. The magnetic isolation layer is sleeved outside the protective inner layer, the protective outer layer is sleeved outside the magnetic isolation layer, and the transmission block is fixedly connected to both ends of the protective outer layer.

[0020] Furthermore, the control component includes a barrel cover, a control component sample holder, a track, a transmission block, and a cylinder; the track is bonded inside the cylinder, the transmission block is fixed to both ends of the outer surface of the cylinder, the control component sample holder is installed on the track, and the barrel cover is buckled to the open end of the cylinder.

[0021] Furthermore, the low-gravity experiment component includes a base component, a transmission component, and a sample tray; the transmission component is installed inside the base component and is used to drive the sub-magnetic experiment component and the control component to rotate according to the experimental requirements; the sample tray is installed on the upper part of the transmission component.

[0022] Furthermore, the base component includes a bottom plate, side plates, a driving support frame, a driven support frame, and bearings. A rectangular box structure with an open upper end is formed by the bottom plate, side plates, driving support frame, and driven support frame. The bottom plate is provided at the bottom of the rectangular box structure. The driving support frame and the driven support frame are provided on a set of opposite sides of the rectangular box. The two side plates are provided on the other set of opposite sides of the rectangular box. The driving support frame and the driven support frame are provided with a plurality of bearing mounting holes, and the bearings are installed in the bearing mounting holes.

[0023] Furthermore, the transmission component includes a motor, a movable seat, a driving pulley, a synchronous belt, a driven pulley, a driving rotating end, and a driven rotating end. The output end of the motor is fixed on the movable seat. The driving pulley is installed on the output shaft of the motor. The movable seat is fixed on the driving support frame. The end shaft of the driving rotating end passes through the inner hole of the bearing on the driving support frame and is installed with the driving support frame. The driving pulley is installed on the end shaft of the driving rotating end. The end shaft of the driven rotating end passes through the inner hole of the bearing on the driven support frame and is installed with the driven support frame. The synchronous belt is installed between the driving pulley and the driven pulley. Sliding grooves for matching with the transmission blocks on the sub-magnetic experiment component and the control component are provided on the end faces of the driving rotating end and the driven rotating end, so as to drive the sub-magnetic experiment component and the control component to rotate.

[0024] Furthermore, the outer shell is composed of a body structure, an upper cover, a rear cover, a hinge, and a heat insulation layer. The body structure is connected to the upper cover through the hinge. The body structure is connected to the rear cover by screws. The heat insulation layer is installed inside the body structure.

[0025] Furthermore, the cell culture environment control component includes a temperature control component and a CO2 concentration control component. The CO2 concentration control component includes a CO2 gas cylinder, a pressure reducing valve, a solenoid valve, a CO2 concentration sensor, and a pressure pipe. The pressure reducing valve is installed on the CO2 gas cylinder. The pressure reducing valve and the solenoid valve are connected through the pressure pipe. The CO2 concentration sensor is installed on the bottom plate of the base component. The temperature control component is used to adjust the temperature of the cell experiment area and includes a heater, a convection fan, and a temperature sensor. The heater and the convection fan are installed on the driven support frame. The temperature sensor is installed at the middle position of the bottom plate of the base component.

[0026] Further, the control system includes a control circuit board and a display screen. The control circuit board is installed on the rear cover of the housing, and the display screen is installed on the upper cover of the housing. The control circuit board is electrically connected to the cell culture environment control component, the low gravity experiment component, and the display screen.

[0027] With the above solution, through the cytological composite effect simulation system based on automatic control, different levels of low gravity and sub-magnetic composite environments can be established, and the automatic control of the cell culture environment can be realized, supporting the research on cytological effects of space low gravity and sub-magnetic composite environments on the ground.

[0028] The above description is only an overview of the technical solution of the present invention. In order to understand the technical means of the present invention more clearly and implement it according to the content of the specification, the following is a detailed description of the preferred embodiment of the present invention in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 is a schematic structural diagram of a cytological composite effect simulation system based on automatic control of the present invention;

[0030] Figure 2 is a schematic structural diagram of the sub-magnetic experiment component of the present invention;

[0031] Figure 3 is a schematic structural diagram of the sample rack of the present invention;

[0032] Figure 4 is a schematic structural diagram of the control component of the present invention;

[0033] Figure 5 is a schematic structural diagram of the low gravity experiment component of the present invention;

[0034] Figure 6 is a schematic structural diagram of the basic component of the present invention;

[0035] Figure 7 is a schematic structural diagram of the transmission component of the present invention;

[0036] Figure 8 is a schematic structural diagram of the housing of the present invention.

[0037] Reference numerals in the drawings:

[0038] 1 - Sub-magnetic experiment component; 11 - End cover; 12 - Experiment component sample rack; 121 - Main structure; 122 - Buffer; 13 - Cylindrical tubular coil; 14 - Magnetic isolation component; 15 - Convection fan; 16 - Slip ring;

[0039] 2 - Control component; 21 - Cylinder cover; 22 - Control component sample rack; 23 - Track; 24 - Transmission block; 25 - Cylinder;

[0040] 3 - Low - gravity experiment component; 31 - Basic component; 311 - Bottom plate; 312 - Side plate; 313 - Active support frame; 314 - Driven support frame; 315 - Bearing; 32 - Transmission component; 321 - Motor; 322 - Movable seat; 323 - Driving pulley; 324 - Timing belt; 325 - Driven pulley; 326 - Active rotating end; 327 - Driven rotating end; 33 - Sample tray;

[0041] 4 - Outer shell; 41 - Body structure; 42 - Upper cover; 43 - Rear cover; 44 - Hinge; 45 - Thermal insulation layer;

[0042] 5 - Control system;

[0043] 6 - Cell culture environment control component. Specific implementation manners

[0044] The following combines the accompanying drawings and embodiments to further describe in detail the specific implementation manners of the present invention. The following embodiments are used to illustrate the present invention, but are not used to limit the scope of the present invention.

[0045] Refer Figure 1 As shown, this embodiment provides a cytological composite effect simulation system based on automatic control, including a sub - magnetic experiment component 1, a control component 2, a low - gravity experiment component 3, an outer shell 4, a control system 5, and a cell culture environment control component 6. Among them, a sub - magnetic environment can be established through the sub - magnetic experiment component 1, and different levels of gravity environments can be simulated through the low - gravity experiment component 3. Combining the sub - magnetic experiment component 1 and the low - gravity experiment component 3 can establish different levels of low - gravity and sub - magnetic composite environments. Combining with the control system 5 and the cell culture environment control component 6 can also realize the automatic control of the cell culture environment. Through this low - gravity and sub - magnetic composite environment simulation system, experiments can be carried out independently without the assistance of other peripheral equipment, and it can realize the research on the cytological effects of space low - gravity and sub - magnetic composite environments on the ground.

[0046] In this embodiment, the sub - magnetic experiment component 1, the control component 2, and the low - gravity experiment component 3 are arranged inside the outer shell 4. Among them, the sub - magnetic experiment component 1 is used to establish a sub - magnetic environment with a low magnetic field intensity that meets the experimental requirements in the area where the cell sample is located; the control component is used to establish an experimental environment for the control sample group of the experiment; the low - gravity experiment component 3 is used to drive the experimental component (the sub - magnetic experiment component 1 in this embodiment) to rotate according to the experimental requirements to simulate the low - gravity environment required by the experiment; the cell culture environment control component 6 is used to realize the temperature and CO2 concentration conditions required for cell experiments under the regulation of the control system 5; the control system 5 is used to regulate the cell culture environment control component 6 and the low - gravity experiment component 3 to work according to the experimental requirements; the outer shell 4 is the installation foundation and protection structure of all the above components.

[0047] Refer Figure 2As shown in the figure, the sub-magnetic experimental component 1 is composed of an end cover 11, an experimental component sample holder 12, a cylindrical tubular coil 13, a magnetic isolation component 14, a convection fan 15, and a slip ring 16. Among them, the slip ring 16 and the convection fan 15 are fixed to one end of the magnetic isolation component 14 by screws; the cylindrical tubular coil 13 is installed inside the magnetic isolation component 14; the experimental component sample holder 12 is installed inside the cylindrical tubular coil 13 through the track inside the cylindrical tubular coil 13 for installing experimental samples; the end cover 11 is buckled to the other end (open end) of the magnetic isolation component 14 to form a closed space inside the magnetic isolation component 14.

[0048] The magnetic isolation component 14 is used to isolate the magnetic field in the experimental environment, including a magnetic isolation layer, a protective outer layer, a protective inner layer, and a transmission block. The magnetic isolation layer is sleeved outside the protective inner layer, the protective outer layer is sleeved outside the magnetic isolation layer, and the transmission blocks are welded to both ends of the protective outer layer. The cylindrical tubular coil 13 is used to generate a uniform magnetic field required for the experiment, including a track, a support tube, and enameled wire spirally wound outside the support tube. The track is bonded to the inner surface of the support tube. The end cover 11 is composed of an inner cover, a magnetic isolation plate, and an outer cover, and the inner cover and the outer cover are respectively bonded to both sides of the magnetic isolation plate.

[0049] See Figure 3 As shown in the figure, the experimental component sample holder 12 includes a main structure 121 and a buffer 122, and the buffer 122 is bonded to the main structure 121.

[0050] See Figure 4 As shown in the figure, the control component 2 includes a cylinder cover 21, a control component sample holder 22, a track 23, a transmission block 24, and a cylinder 25. The track 23 is bonded inside the cylinder 25, the transmission blocks 24 are welded to both ends of the outer surface of the cylinder 25, the control component sample holder 22 is installed on the track 23, and the cylinder cover 21 is buckled to the open end of the cylinder 25.

[0051] See Figure 5 As shown in the figure, the low-gravity experimental component 3 includes a base component 31, 4 sets of transmission components 32 (which can be increased or decreased according to experimental needs), and a sample tray 33. The transmission components 32 are installed inside the base component 31, and the sample tray 33 is installed on the upper part of the transmission components 32. The base component 31 is the installation base of the low-gravity experimental component 3 and is used to support the transmission components 32 and the sample tray 33 in the low-gravity experimental component 3. The transmission components 32 are used to drive the sub-magnetic experimental component 1 and the control component 2 to rotate according to experimental requirements.

[0052] See Figure 6As shown, the base component 31 includes a bottom plate 311, side plates 312, a driving support frame 313, a driven support frame 314, and bearings 315. The base component 31 is composed of the bottom plate 311, side plates 312, driving support frame 313, and driven support frame 314 to form a rectangular box structure with an open upper end. The bottom plate 311 is provided at the bottom surface of the rectangular box structure. The driving support frame 313 and the driven support frame 314 are provided on a set of opposite sides of the rectangular box. Two side plates 312 are provided on the other set of opposite sides of the rectangular box. The driving support frame 313 and the driven support frame 314 are provided with a plurality of bearing mounting holes, and the bearings 315 are installed in the bearing mounting holes on the driving support frame 313 and the driven support frame 314.

[0053] Refer Figure 7 As shown, the transmission component 32 includes a motor 321, a movable seat 322, a driving pulley 323, a synchronous belt 324, a driven pulley 325, a driving rotating end 326, and a driven rotating end 327. The output end of the motor 321 is fixed to the movable seat 322 by screws. The driving pulley 323 is installed on the output shaft of the motor 321. The movable seat 322 with the motor 321 and the driving pulley 323 is fixed to the driving support frame 313 by screws. The driving rotating end 326 is installed with the driving support frame 313 by passing its end shaft through the bearing inner hole of the driving support frame 313. The driving pulley 323 is installed on the end shaft of the driving rotating end 326. The driven rotating end 327 is installed with the driven support frame 314 by passing its end shaft through the bearing inner hole of the driven support frame 314. The synchronous belt 324 is installed between the driving pulley 323 and the driven pulley 325. The end faces of the driving rotating end 326 and the driven rotating end 327 are both provided with sliding grooves matching the transmission blocks on the sub-magnetic experimental component 1 and the control component 2, so as to drive the sub-magnetic experimental component 1 and the control component 2 to rotate.

[0054] Refer Figure 8 As shown, the outer shell 4 is composed of a body structure 41, an upper cover 42, a rear cover 43, a hinge 44, and a heat insulation layer 45. The body structure 41 is connected to the upper cover 42 by the hinge 44. The body structure 41 is connected to the rear cover 43 by screws. The heat insulation layer 45 is installed inside the body structure 41.

[0055] The cell culture environment control component 6 includes a temperature control component and a CO2 concentration control component. The CO2 concentration control component includes a CO2 gas cylinder, a pressure reducing valve, a solenoid valve, a CO2 concentration sensor, and a pressure pipe. The pressure reducing valve is installed on the CO2 gas cylinder, and the pressure reducing valve is connected to the solenoid valve through the pressure pipe. The CO2 concentration sensor is installed on the bottom plate 311 of the base component 31. After the CO2 gas in the CO2 gas cylinder is depressurized by the pressure reducing valve, it flows into the solenoid valve through the pressure pipe. The control system 5 adjusts the opening of the solenoid valve. After the solenoid valve is opened, the CO2 gas flows into the cell experiment area surrounded by the base component 31 through the pipeline. After being detected by the CO2 concentration sensor and the CO2 concentration meets the experimental requirements, the control system 5 controls the solenoid valve to close, the CO2 gas is cut off, and the inflow into the cell experiment area surrounded by the base component 31 stops. The temperature control component consists of a heater, a convection fan, and a temperature sensor, and is used to adjust the temperature of the cell experiment area. Among them, the heater and the convection fan are installed on the driven support frame 314, and the temperature sensor is installed at the middle position of the base component bottom plate 311.

[0056] The control system 5 consists of several control circuit boards and a display screen. The control circuit boards are installed on the back (rear cover) of the housing 4, and the display screen is installed on the upper cover of the housing 4. The control circuit boards are connected to the cell culture environment control component, the low gravity experiment component, and the display screen through wires.

[0057] In a specific embodiment, both the inner cover and the outer cover of the end cap 11 are processed from ABS material. The outer cover has a diameter of 120 mm and a thickness of 5 mm, and the inner cover has a diameter of 85 mm and a thickness of 5 mm. The magnetic separation plate is processed into a circular sheet from 1J85 material, with a diameter of 85 mm and a thickness of 1 mm, and is coaxially bonded together in the order of the outer cover, the magnetic separation plate, and the inner cover with 502 glue.

[0058] The main structure 121 of the experimental component sample rack 12 is formed by 3D printing with resin material, and 2 T25 cell culture bottles can be installed (for the installation requirements of other specifications of experimental samples, it can be achieved by adjusting the size of the main structure). The silk surface of the nylon hook and loop is used as the buffer cushion layer (buffer member 122), and after being cut, it is pasted on the inner surface of the main structure 121 with 502 glue to ensure that the T25 cell culture bottle will not slip off when the experimental component sample rack 12 moves. The control component sample rack 22 can adopt the same structure as the experimental component sample rack 12, which will not be elaborated here.

[0059] The support tube of the cylindrical tubular coil 13 is made of plexiglass material, with an inner diameter of 85 mm, a wall thickness of 2 mm, and a length of 400 mm. A layer of enameled wire with a diameter of 3 mm is spirally wound on the outer surface (multiple layers of enameled wire with other diameters can also be wound); the grooved track is machined from ABS material and is pasted on the inner surface of the support tube with 502 glue, and the track and the conduction block are installed on the same plane.

[0060] The magnetic isolation layer of the magnetic isolation component 14 is made of 1J85 sheet with a thickness of 1 mm, bent into a circular tube and welded by argon arc welding. The inner diameter of the magnetic isolation layer is 95 mm and the length is 400 mm (it can also be bent into multiple magnetic isolation layers according to needs); the protective outer layer is machined from aluminum alloy 2A12 material, with an inner diameter of 104 mm, an outer diameter of 120 mm, and a length of 430 mm; the protective inner layer is machined from aluminum alloy 2A12 material, with an inner diameter of 95 mm, an outer diameter of 102 mm, and a length of 400 mm; the drive block is machined from aluminum alloy 2A12 material, with a length of 20 mm and a thickness of 10 mm, and is welded to both ends of the outer surface of the protective outer layer by argon arc welding.

[0061] The convection fan 15 adopts a 40406 series ultra - quiet convection fan, with an outer dimension of 40 mm × 40 mm × 6 mm, a working voltage of 12 DCV, and is fixed to one end of the protective outer layer of the magnetic isolation component 14 with 4 M3 screws.

[0062] The slip ring 16 adopts a 4 - way cylindrical micro - slip ring with a diameter of 5 mm and a length of 11.4 mm. 2 ways are used to supply power to the cylindrical tubular coil 13, and 2 ways are used to supply power to the convection fan 15. The slip ring 16 is bonded to the center of one end of the protective outer layer of the magnetic isolation component 14 with 502 glue.

[0063] The cylinder 25 of the control component 2 is formed by machining from ABS material, with an inner diameter of 85 mm, an outer diameter of 95 mm, and a length of 400 mm; the internal track 23 is also machined from ABS material with a length of 400 mm and is bonded inside the cylinder 25 with 502 glue; the drive block 24 is made of ABS, machined into shape, with a length of 20 mm and a thickness of 10 mm, and is bonded to both ends of the outer surface inside the cylinder 25 with 502 glue. The track 23 and the drive block 24 are on the same plane.

[0064] In the basic component 31 of the low - gravity experiment component 3, both the active support frame 313 and the driven support frame 314 are formed by machining from aluminum alloy material 2A12, with an outer dimension of 490 mm × 200 mm × 30 mm, and the internal hollow area dimension is 480 mm × 200 mm × 20 mm. There are 8 bearing holes distributed on the upper part, and the hole diameter is There are 8 rolling bearings 315 on each of the active support frame 313 and the driven support frame 314. The outer diameter of the bearing 315 is 20 mm, the inner diameter is 8 mm, and the thickness is 5 mm; the bottom plate 311 is processed from 2A12 material, with a length of 510 mm, a width of 490 mm, and a thickness of 10 mm; the 2 side plates 312 are both formed by machining from 2A12, with an outer dimension of 510 mm × 200 mm × 3 mm. The active support frame 313 and the driven support frame 314 are each fixed to both ends of the bottom plate 311 with 4 M4 screws, and the 2 side plates 312 are fixed to the sides of the active support frame 313 and the driven support frame 314 with M3 screws.

[0065] The motor 321 of the transmission component 32 is a 24V DC motor with an outer diameter of 38mm and a length of 60mm; the movable seat 322 is machined from aluminum alloy 2A12, with an outer diameter of 50mm, a length of 30mm, and an inner diameter of 38mm; the driving pulley 323 is machined from aluminum alloy 2A12, has 24 T-shaped teeth, a pitch diameter of 28mm, and a thickness of 10mm; the driven pulley 325 is machined from aluminum alloy 2A12, has 72 T-shaped teeth, a pitch diameter of 84mm, and a thickness of 10mm; the synchronous belt 324 is an XL series light-load belt with a bandwidth of 8mm; the driving rotating end 326 and the driven rotating end 327 are both machined from aluminum alloy 2A12, with an outer diameter of 120mm and a thickness of 10mm. One end of each has a chute matching the transmission blocks on the sub-magnetic experiment component 1 and the control component 2, with a chute width of 10mm, and the other end has a straight shaft with a diameter of 8mm and a length of 35mm. The motor 321 is fixed in the movable seat 322 with 2 M3 set screws. After the driving pulley 3223 is installed on the motor shaft, the movable seat 322 is installed at the lower part of one side of the driving support frame 313 and fixed with 4 M3 screws. After the shaft of the driving rotating end 326 passes through the bearing hole in the upper part of the driving support frame 313, the driven pulley 325 is installed on it and bonded with 502 to prevent slipping. The synchronous belt 324 is installed between the driving pulley 323 and the driven pulley 325. The driven rotating end 327 is installed on the driven support frame 314 through the end shaft on it passing through the bearing inner hole of the driven support frame 314, and is also bonded with 502 to prevent slipping.

[0066] The sample tray 33 is stamped from a 1mm thick stainless steel plate, and small holes with a diameter of 10mm are evenly distributed on the bottom of the tray.

[0067] The main body structure 41, the upper cover 42, and the rear cover 43 of the outer shell 4 are all formed by 3D printing with photosensitive resin, with a wall thickness of 3mm. The upper cover 42 and the rear cover 43 are fixed to the main body structure 41 with M3 screws; the thermal insulation layer 45 is integrally foamed from polyurethane foam material, and the thermal insulation layer 45 is installed inside the main body structure 41.

[0068] The CO2 gas cylinder in the cell culture environment control component 6 is an aluminum flat-bottom 2L pressure bottle, the pressure reducing valve is made of bipolar stainless steel, the solenoid valve is a 12V normally closed pneumatic solenoid valve, the CO2 concentration sensor is an infrared detection type sensor with a measurement range of 0 - 10%, and the pressure pipe is a 3mm outer diameter polytetrafluoroethylene hard gas pipe with an inner diameter of 1mm. The solenoid valve and the CO2 concentration sensor are both fixed on the bottom plate with 2 M2 screws.

[0069] The heater in the temperature control component uses a polyimide film heater, with a heating voltage of 24V, a heating resistance of 24Ω, and a size of 40mm◇40mm. It is directly attached to the lower part of the driven support 314 through back glue; a 12V convection fan is installed in front of the polyimide film heater and fixed with 4 M3 screws; the temperature sensor uses a standard digital sensing module and is installed at the middle position of the bottom plate 311 through 4 M2 screws.

[0070] The working mode of the cytology composite effect simulation system based on automatic control is as follows:

[0071] Install 2 sub-magnetic experiment components 1 and 2 control components 2 on the transmission component 32. Each sub-magnetic experiment component 1 and control component 2 is installed with 3 sample racks to accommodate 6 T25 cell culture bottles. A total of 24 T25 samples are installed on the 4 components. Multiple spare T25 cell samples can be placed on the sample tray 33 as spare experimental samples. Close the upper cover 42 to form a closed experimental area inside the device. Enter the experimental parameters as required on the display screen. The control system 5 will control the rotation of 1 sub-magnetic experiment component 1 and 1 control component 2 according to the set parameters, and the other 1 sub-magnetic experiment component 1 and 1 control component 2 will remain stationary. Then, experimental samples in four states, namely the normal experimental group, the low-gravity experimental group, the sub-magnetic experimental group, and the low-gravity and sub-magnetic composite experimental group, can be established synchronously, as well as spare samples cultured in the same environment. At the same time, under the regulation of the control system 5, the temperature in the cell experiment area is controlled within the range of 37°C ± 0.5°C, and the CO2 concentration is controlled within the range of 5% ± 0.3%. An environmental condition suitable for cell growth is formed in the cell experiment area to ensure the long-term development of relevant experiments. During the experiment, the experimental status is displayed on the display screen in real time.

[0072] The present invention has the following technical effects:

[0073] 1) It can establish low-gravity experimental environments at different levels;

[0074] 2) It can establish a uniform sub-magnetic environment with different magnetic field intensities that meets the requirements of cytology experiments;

[0075] 3) It can establish a cytology experimental environment with the combination of low gravity and sub-magnetism;

[0076] 4) It can simultaneously establish experimental samples in five states, namely the normal experimental group, the normal control group, the low-gravity experimental group, the sub-magnetic experimental group, and the low-gravity and sub-magnetic composite experimental group;

[0077] 5) It can autonomously regulate the environmental parameters in the cell experiment area during the experiment to meet the requirements of cell growth and realize the long-term development of relevant experiments.

[0078] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. It should be noted that for those of ordinary skill in the art, without departing from the technical principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A cytological composite effect simulation system based on automatic control, characterized in that, It includes a sub-magnetic experiment component, a control component, a low-gravity experiment component, a housing, a cell culture environment control component, and a control system; the housing is of a box structure, and the sub-magnetic experiment component, the control component, and the low-gravity experiment component are arranged inside the housing, and a closed cell experiment area is formed inside the housing; The sub-magnetic experiment component is used to establish a sub-magnetic environment with a low magnetic field intensity that meets the experimental requirements in the area where the cell sample is located; The control component is used to establish an experimental environment for the control sample group of the experiment; The low-gravity experiment component is used to drive the sub-magnetic experiment component to rotate according to the experimental requirements to simulate the low-gravity environment required by the experiment; The cell culture environment control component is used to establish the temperature and CO2 concentration conditions required for cell experiments in the cell experiment area; The control system is used to regulate the cell culture environment control component and the low-gravity experiment component to work according to the experimental requirements; The control component includes a cylinder cover, a control component sample rack, a track, a transmission block, and a cylinder; the track is bonded inside the cylinder, the transmission blocks are fixed at both ends of the outer surface of the cylinder, the control component sample rack is installed on the track, and the cylinder cover is buckled at the open end of the cylinder; The low-gravity experiment component includes a basic component, a transmission component, and a sample tray; the transmission component is installed inside the basic component and is used to drive the sub-magnetic experiment component and the control component to rotate according to the experimental requirements; the sample tray is installed on the upper part of the transmission component.

2. The cytology composite effect simulation system based on automatic control according to claim 1, wherein The sub-magnetic experiment component includes an end cover, an experiment component sample rack, a cylindrical tubular coil, a magnetic isolation component, a convection fan, and a slip ring; the slip ring and the convection fan are fixed at one end of the magnetic isolation component; the cylindrical tubular coil is installed inside the magnetic isolation component; the experiment component sample rack is installed inside the cylindrical tubular coil through the track inside the cylindrical tubular coil; the end cover is buckled at the other end of the magnetic isolation component.

3. The cytological composite effect simulation system based on automatic control according to claim 2, wherein, The end cover is composed of an inner cover, a magnetic isolation plate, and an outer cover, and the inner cover and the outer cover are respectively bonded to both sides of the magnetic isolation plate; The experiment component sample rack is used to install experimental samples and includes a main structure and a buffer, and the buffer is bonded to the main structure; The cylindrical tubular coil is used to generate a uniform magnetic field required by the experiment and includes a track, a support tube, and an enameled wire spirally wound outside the support tube, and the track is bonded to the inner surface of the support tube; The magnetic isolation component is used to isolate the magnetic field in the experimental environment and includes a magnetic isolation layer, a protective outer layer, a protective inner layer, and a transmission block. The magnetic isolation layer is sleeved outside the protective inner layer, the protective outer layer is sleeved outside the magnetic isolation layer, and the transmission block is fixedly connected to both ends of the protective outer layer.

4. The cytology composite effect simulation system based on automatic control according to claim 1, wherein The basic components include a bottom plate, side plates, an active support frame, a driven support frame, and bearings. A rectangular box structure with an open upper end is formed by the bottom plate, side plates, active support frame, and driven support frame. The bottom plate is provided at the bottom of the rectangular box structure. The active support frame and the driven support frame are provided on a set of opposite sides of the rectangular box. The two side plates are provided on the other set of opposite sides of the rectangular box. The active support frame and the driven support frame are provided with a plurality of bearing mounting holes, and the bearings are mounted in the bearing mounting holes.

5. The cytology composite effect simulation system based on automatic control according to claim 4, characterized in that The transmission components include a motor, a movable seat, an active pulley, a synchronous belt, a driven pulley, an active rotating end, and a driven rotating end. The output end of the motor is fixed on the movable seat. The active pulley is mounted on the output shaft of the motor. The movable seat is fixed on the active support frame. The end shaft of the active rotating end passes through the inner hole of the bearing on the active support frame and is mounted with the active support frame. The active pulley is mounted on the end shaft of the active rotating end. The end shaft of the driven rotating end passes through the inner hole of the bearing on the driven support frame and is mounted with the driven support frame. The synchronous belt is mounted between the active pulley and the driven pulley. Sliding grooves for matching with the transmission blocks on the sub-magnetic experimental component and the control component are provided on the end faces of the active rotating end and the driven rotating end to drive the sub-magnetic experimental component and the control component to rotate.

6. The cytology composite effect simulation system based on automatic control according to claim 1, characterized in that The outer shell is composed of a body structure, an upper cover, a rear cover, a hinge, and a heat insulation layer. The body structure is connected to the upper cover through the hinge. The body structure is connected to the rear cover by screws. The heat insulation layer is installed inside the body structure.

7. The cytology composite effect simulation system based on automatic control according to claim 4, wherein The cell culture environment control component includes a temperature control component and a CO2 concentration control component. The CO2 concentration control component includes a CO2 gas cylinder, a pressure reducing valve, a solenoid valve, a CO2 concentration sensor, and a pressure pipe. The pressure reducing valve is mounted on the CO2 gas cylinder. The pressure reducing valve and the solenoid valve are connected through the pressure pipe. The CO2 concentration sensor is mounted on the bottom plate of the basic component. The temperature control component is used to adjust the temperature of the cell experiment area and includes a heater, a convection fan, and a temperature sensor. The heater and the convection fan are mounted on the driven support frame. The temperature sensor is mounted at the middle position of the bottom plate of the basic component.

8. The cytology composite effect simulation system based on automatic control according to claim 6, characterized in that, The control system includes a control circuit board and a display screen. The control circuit board is mounted on the rear cover of the outer shell. The display screen is mounted on the upper cover of the outer shell. The control circuit board is electrically connected to the cell culture environment control component, the low gravity experiment component, and the display screen.

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