Method for treating brewing wastewater residue using microbial agents

Abstract

The present invention relates to the field of brewery wastewater residue treatment, specifically a method for treating brewery wastewater residue using a microbial agent. To address the problem of large waste residue production and environmental pollution during brewery wastewater treatment, the present invention provides a method for treating brewery wastewater residue, comprising the following steps: a. Activating Lactobacillus, Bacillus, Aspergillus, and Trichoderma, adding barley grains, sorghum leaves, sugarcane leaves, and water, and incubating at room temperature for 10 to 20 days to prepare a microbial agent; b. Adding the microbial agent and auxiliary agents to the brewery wastewater residue and mixing thoroughly; c. Fermenting, wherein the temperature begins to rise after 1 to 3 days, reaching a maximum temperature of 62 to 73°C in 2 to 7 days, maintaining the maximum temperature for 7 to 15 days, and then fermenting for an additional 10 to 13 days, for a total fermentation time of 20 to 38 days. The present method has a short fermentation cycle, produces no odor during the fermentation process, reduces the moisture content of the residue to below 35%, and poses no secondary environmental pollution, thus having significant practical significance.

Description

Technical Field

[0001] The invention belongs to the field of brewing wastewater bacterial residue treatment, and specifically relates to a method for treating brewing wastewater bacterial residue by utilizing a microbial agent. Background Art

[0002] The wastewater generated during the brewing process mainly includes brewing bottom pot water, flushing water and condensate. In the process of brewing wastewater treatment, it is necessary to add bacteria and auxiliary agents. The bacteria mainly include nitrifying bacteria, denitrifying bacteria, Bacillus subtilis, etc.; the auxiliary agents are sodium hydroxide, polyferric sulfate and polyacrylamide. A large amount of bacterial residue organic matter is produced during the wastewater treatment process. Its main components and sources are (1) untreated starch, reducing sugars, organic acids, esters, alcohols and other large molecular refractory organic matter in the brewing wastewater, and (2) microbial residues produced during the wastewater treatment process.

[0003] Mushroom residue contains a large amount of organic matter (about 70%), nutrients such as nitrogen and phosphorus, and a small amount of harmful substances. It is in a semi-solid state, has a high yield, a high moisture content, poor dehydration, is difficult to store, and is prone to corruption. If not handled promptly and effectively, it will cause environmental pollution. Currently, the commonly used treatment methods at home and abroad include landfill, incineration, thermal treatment, and composting. Incineration of mushroom residue produces smoke, dust, and other substances, causing environmental pollution; landfilling of mushroom residue wastes resources; because mushroom residue contains high phosphorus and has an unsuitable carbon-nitrogen ratio, if the mushroom residue is applied directly to the soil without fermentation, it is not only easy to burn the seedlings, but also causes nutrient leaching into the groundwater, causing secondary pollution. Current biological treatment technology is still immature, mostly relying on single microbial fermentation treatment, which takes a long time, produces a large amount of odor during the fermentation process, pollutes the environment, and has cumbersome treatment processes, strict operating techniques, and large equipment investments. Although adding chemical adjuvants can shorten the fermentation time, it will cause secondary pollution to the environment.

[0004] Therefore, the existing treatment methods have too high treatment costs and are difficult to promote and apply in the brewing industry; in order to ensure that the organic matter in the bacterial residue in brewing wastewater is harmlessly and stably treated, an economical and effective method for the organic matter in the bacterial residue in brewing wastewater is needed. Summary of the Invention

[0005] The technical problem to be solved by the present invention is that the waste residue produced during the treatment of brewing wastewater is large in output, pollutes the environment and cannot be recycled as a resource.

[0006] The present invention solves the above technical problems by providing a method for treating brewing wastewater residue using microbial agents. The method comprises the following steps:

[0007] a. Activate Lactobacillus, Bacillus, Aspergillus and Trichoderma, add barley grains, sorghum leaves, sugarcane leaves and water, and culture at room temperature for 10 to 20 days to prepare a microbial agent;

[0008] b. Add the microbial agent and adjuvant obtained in step a to the brewing wastewater residue and mix well;

[0009] c. Ferment the mixed material. Start to heat up after 1 to 3 days. The temperature reaches the highest temperature of 62 to 73°C in 2 to 7 days. Maintain the highest temperature for 7 to 15 days. Ferment for another 10 to 13 days. The total fermentation time is 20 to 38 days.

[0010] Among them, in the above-mentioned method for treating brewing wastewater residue using microbial agents, the lactobacillus described in step a is Lactobacillus acidophilus; the Bacillus is a mixture of Bacillus subtilis and Bacillus licheniformis in a weight ratio of 1 to 4:1 to 4; the Aspergillus is Aspergillus niger; and the Trichoderma is a mixture of Trichoderma harzianum and Trichoderma viride in a weight ratio of 1 to 8:1 to 8.

[0011] Among them, in the above-mentioned method for treating brewery wastewater residue with microbial agents, the activation of Lactobacillus and Bacillus in step a refers to culturing the cells in liquid beef extract peptone medium to a concentration of ≥10 8 / mL.

[0012] Among them, in the above-mentioned method for treating brewing wastewater residue with microbial agents, the activation of Aspergillus niger and Trichoderma spp. in step a refers to culturing the Aspergillus niger and Trichoderma spp. in a potato sucrose medium to a spore concentration of ≥10 8 / mL.

[0013] Among them, in the above-mentioned method of treating brewing wastewater residue using microbial agents, the composition of the microbial agent described in step a includes: by weight, 2 to 8 parts of Lactobacillus, 1 to 5 parts of Bacillus, 4 to 10 parts of Aspergillus, 2 to 6 parts of Trichoderma, 5 to 15 parts of barley grains, 10 to 28 parts of sorghum leaves, 8 to 18 parts of sugarcane leaves, and 30 to 50 parts of water.

[0014] Preferably, in the above-mentioned method for treating brewing wastewater residue using microbial agents, the composition of the microbial agent described in step a includes: by weight, 5 parts of Lactobacillus, 3 parts of Bacillus, 6 parts of Aspergillus, 3 parts of Trichoderma, 10 parts of barley grains, 20 parts of sorghum leaves, 13 parts of sugarcane leaves, and 40 parts of water.

[0015] Preferably, in the above method for treating brewing wastewater residue using microbial agents, the room temperature culture in step a is for 14 days.

[0016] Among them, in the above-mentioned method of treating brewing wastewater residue using microbial agents, the particle size of the sorghum leaves and sugarcane leaves described in step a is 3 to 8 cm.

[0017] Among them, in the above-mentioned method of treating brewing wastewater residue using microbial agents, the composition of the auxiliary agent described in step b includes: 60 to 90 parts of calcium sulfate and 10 to 40 parts of urea in parts by weight.

[0018] Preferably, in the above method for treating brewing wastewater residue using microbial agents, the auxiliary agent described in step b comprises: 70 parts by weight of calcium sulfate and 30 parts of urea.

[0019] Wherein, in the above method for treating brewing wastewater residue using microbial agents, the weight ratio of the microbial agent to the residue in step b is 1:50-100, preferably 1:80.

[0020] Wherein, in the above method for treating brewing wastewater residue with microbial agents, the weight ratio of the auxiliary agent to the residue in step b is 1:500 to 2000, preferably 1:1000.

[0021] Preferably, in the above method of treating brewing wastewater residue using microbial agents, the fermentation conditions in step c are: start heating after 1 day, reach a maximum temperature of 73°C after 2 days, maintain the maximum temperature for 10 days, and then ferment for 10 days, with a total fermentation time of 23 days.

[0022] Among them, in the above-mentioned method of treating brewing wastewater residue using microbial agents, the pile is turned over once every 1 to 4 days during fermentation in step c, preferably once every 3 days.

[0023] The beneficial effects of the present invention are:

[0024] The present invention utilizes microbial agents to treat bacterial residue in brewery wastewater. By adding microbial agents and optimizing the fermentation process, the organic matter in the bacterial residue generated during the brewery wastewater treatment process is rendered harmless. During the fermentation process, aerobic microorganisms grow rapidly, the temperature rises quickly, the high-temperature phase lasts relatively long, and the fermentation cycle is short, only 23 days. No odor is generated during the fermentation process, the moisture content in the bacterial residue is reduced to below 35%, and there is no secondary pollution to the environment. The treatment process of the present invention is simple and faster, and the synergistic effect generated between microorganisms improves the treatment effect of bacterial residue in brewery wastewater. DETAILED DESCRIPTION

[0025] The present invention provides a method for treating brewing wastewater residue using a microbial agent, comprising the following steps:

[0026] a. Activate Lactobacillus, Bacillus, Aspergillus and Trichoderma, add barley grains, sorghum leaves, sugarcane leaves and water, and culture at room temperature for 10 to 20 days to prepare a microbial agent;

[0027] b. Add the microbial agent and adjuvant obtained in step a to the brewing wastewater residue and mix well;

[0028] c. Ferment the mixed material. Start to heat up after 1 to 3 days. The temperature reaches the highest temperature of 62 to 73°C in 2 to 7 days. Maintain the highest temperature for 7 to 15 days. Ferment for another 10 to 13 days. The total fermentation time is 20 to 38 days.

[0029] Among them, in the above-mentioned method of treating brewing wastewater residue using microbial agents, in order to achieve better fermentation effect, the lactobacillus described in step a is Lactobacillus acidophilus; the Bacillus is a mixture of Bacillus subtilis and Bacillus licheniformis mixed in a weight ratio of 1 to 4:1 to 4; the Aspergillus is Aspergillus niger; and the Trichoderma is a mixture of Trichoderma harzianum and Trichoderma viride mixed in a weight ratio of 1 to 8:1 to 8.

[0030] Among them, in the above-mentioned method for treating brewery wastewater residue with microbial agents, the activation of Lactobacillus and Bacillus in step a refers to culturing the cells in liquid beef extract peptone medium to a concentration of ≥10 8 / mL.

[0031] Among them, in the above-mentioned method for treating brewing wastewater residue with microbial agents, the activation of Aspergillus niger and Trichoderma spp. in step a refers to culturing the Aspergillus niger and Trichoderma spp. in a potato sucrose medium to a spore concentration of ≥10 8 / mL.

[0032] Among them, in the above-mentioned method of treating brewing wastewater residue using microbial agents, the composition of the microbial agent described in step a includes: by weight, 2 to 8 parts of Lactobacillus, 1 to 5 parts of Bacillus, 4 to 10 parts of Aspergillus, 2 to 6 parts of Trichoderma, 5 to 15 parts of barley grains, 10 to 28 parts of sorghum leaves, 8 to 18 parts of sugarcane leaves, and 30 to 50 parts of water.

[0033] Preferably, in the above-mentioned method for treating brewing wastewater residue using microbial agents, the composition of the microbial agent described in step a includes: by weight, 5 parts of Lactobacillus, 3 parts of Bacillus, 6 parts of Aspergillus, 3 parts of Trichoderma, 10 parts of barley grains, 20 parts of sorghum leaves, 13 parts of sugarcane leaves, and 40 parts of water.

[0034] The present invention formulates a special microbial inoculant, and the fermentation process involves microbial growth, raw material degradation, oxygen consumption, substrate utilization, and the accumulation of water-soluble small molecules. In an ecosystem, microorganisms not only interact with environmental factors but also interact with other microorganisms. The present invention utilizes mixed bacterial fermentation, inoculated with specific functional bacteria to increase the degradation of organic matter. Molds and bacteria are the most important fermenting microorganisms. A key function of molds is to secrete hydrolytic enzymes to degrade macromolecules, providing substrate for bacterial growth and fermentation, while bacteria convert the substrate into small organic molecules. During the entire fermentation process, Aspergillus fuciformis grows rapidly, produces large amounts of organic acids, and effectively inhibits the growth of other bacteria. It also secretes acidic proteases and amylases with high acid resistance. Trichoderma spp. has strong ecological adaptability, diverse reproduction methods, and high reproductive capacity. It can produce a variety of biologically active enzymes, and its cellulase activity is the highest among many microorganisms. It secretes cellulase that degrades cellulose in the bacterial residue mixture. During the high-temperature stage, thermophilic fungi, lactobacilli, and Bacillus spp. are the main pathogens. Lactobacillus and Bacillus are important nutrient-degrading and releasing bacteria that can decompose starch, sugar, and protein. The various microorganisms are not antagonistic to each other and can even play a synergistic role. The microorganisms exert a collective effect, ultimately forming a stable, broadly functional microbial community that can promote the decomposition of organic matter in the fungus residue during the fermentation process. The addition of barley grains to the inoculum provides the composite microorganisms with the necessary nutrients, resulting in a high number of viable bacteria, which persists for a long time. On the one hand, sugarcane leaves and barley leaves can serve as adsorption and coating carriers, providing sufficient growth space for effective bacteria, promoting the growth of effective viable bacteria and improving survival rates. On the other hand, sugarcane leaves and barley leaves act as loosening agents in the fermentation process, increasing the oxygen supply for microbial growth. Aerobic bacteria multiply in large numbers, can better maintain the high-temperature process, avoid anaerobic fermentation, and reduce the generation of odors. The composite microbial inoculum used in the present invention can enable the bacterial colony to grow rapidly in the initial stage of fermentation, maintain high temperatures for a long time, and thus shorten the fermentation time.

[0035] Preferably, in the above method for treating brewing wastewater residue using microbial agents, the room temperature culture in step a is for 14 days.

[0036] Among them, in the above-mentioned method of treating brewing wastewater residue using microbial agents, in order to better utilize nutrients, the particle size of the sorghum leaves and sugarcane leaves described in step a is 3 to 8 cm.

[0037] Among them, in the above-mentioned method of treating brewing wastewater residue using microbial agents, the composition of the auxiliary agent described in step b includes: 60 to 90 parts of calcium sulfate and 10 to 40 parts of urea by weight.

[0038] Preferably, in the above method for treating brewing wastewater residue using microbial agents, the auxiliary agent described in step b comprises: 70 parts by weight of calcium sulfate and 30 parts of urea.

[0039] Wherein, in the above method for treating brewing wastewater residue using microbial agents, the weight ratio of the microbial agent to the residue in step b is 1:50-100, preferably 1:80.

[0040] Wherein, in the above method for treating brewing wastewater residue with microbial agents, the weight ratio of the auxiliary agent to the residue in step b is 1:500 to 2000, preferably 1:1000.

[0041] Preferably, in the above method of treating brewing wastewater residue using microbial agents, the fermentation conditions in step c are: start heating after 1 day, reach a maximum temperature of 73°C after 2 days, maintain the maximum temperature for 10 days, and then ferment for 10 days, with a total fermentation time of 23 days.

[0042] Among them, in the above-mentioned method of treating brewing wastewater residue using microbial agents, the pile is turned over once every 1 to 4 days during fermentation in step c, preferably once every 3 days.

[0043] The present invention specifically sets the range of parameters such as fermentation temperature, fermentation days, and compost turning time. Microorganisms, especially aerobic microorganisms, need to be provided with oxygen for metabolism during their growth and reproduction. If ventilation is poor, the growth of aerobic microorganisms will be inhibited, resulting in a reduction in their number and species, which in turn leads to anaerobic fermentation, not only incomplete decomposition of organic matter, but also the generation of odors. If the ventilation is too strong, the water content of the material will be lost too quickly, the organic matter will be strongly decomposed, and nutrients such as ammonia will be lost, resulting in a reduction in the total amount of organic matter in the material. Therefore, the present invention has determined through experiments that ventilation is carried out by turning the compost, and the compost is turned once every 1 to 4 days to provide oxygen for the fermentation process.

[0044] The temperature of the fermentation process is a key factor affecting microbial fermentation. Under the action of microorganisms, the temperature of the material will change, especially when the microorganisms multiply in large quantities and when the microbial activity intensifies, the material temperature will increase. The general fermentation process is divided into a heating stage (below 50°C), a high temperature stage (50-70°C) and a cooling stage (below 50°C). The fermentation process of the present invention is to ferment the mixed material, start heating after 1 to 3 days, reach a maximum temperature of 62 to 73°C in 2 to 7 days, maintain the maximum temperature for 7 to 15 days, and then ferment for 10 to 13 days, with a total fermentation time of 20 to 38 days. During the fermentation process, it is also necessary to control the material temperature not to be too high, so as not to affect the reproduction of microorganisms. The present invention cools the material by turning the pile once every 1 to 4 days.

[0045] The present invention discloses a method for treating brewery wastewater residue using microbial agents. Microbial agents are added separately for the fermentation process, thereby inhibiting the growth of anaerobic microorganisms and yeasts, allowing aerobic microorganisms to grow rapidly, heating up quickly, starting to heat up within 24 hours, and quickly reaching the target temperature. The high-temperature stage also lasts for a long time. The fermentation process is optimized by adding microbial agents containing lactobacillus, bacillus, aspergillus, and trichoderma. The residue organic matter in brewery wastewater is fully fermented, its water content is reduced to below 35%, and the fermentation cycle is shortened to 23 days. The method is easy to operate in terms of raw material mixing and fermentation process, with a short fermentation cycle. The residue organic matter is fermented thoroughly without generating odor. The fermentation product has a low water content, is easy to transport and store, and does not cause secondary pollution to the environment. The treatment method of the present invention is simple, the process is faster, and the treatment effect of the residue organic matter is improved through the synergistic effect generated between microorganisms.

[0046] The specific embodiments of the present invention will be further explained below through examples, but it is not intended to limit the scope of protection of the present invention to the scope described in the examples.

[0047] Example 1: Treatment of bacterial residue in brewing wastewater using the method of the present invention

[0048] The specific steps are as follows:

[0049] a. Lactobacillus and Bacillus were cultured in liquid beef extract peptone medium until the bacterial concentration reached 10 8 / mL, Aspergillus and Trichoderma were cultured in potato sucrose medium respectively until the spore concentration reached 10 8 / mL;

[0050] The microbial agent raw materials are prepared, by weight, including 3 parts of Lactobacillus acidophilus, 3 parts of Bacillus, 7 parts of Aspergillus niger, 4 parts of Trichoderma, 12 parts of barley grains, 18 parts of sorghum leaves, 15 parts of sugarcane leaves and 38 parts of water; and the mixture is cultured at room temperature for 17 days to prepare a microbial agent.

[0051] b. Add 20 kg of microbial agent and 1.25 kg of auxiliary agent to 1000 kg of brewing wastewater residue and mix well;

[0052] The auxiliary agent comprises: by weight, 60 parts of calcium sulfate and 40 parts of urea;

[0053] c. Ferment the mixed material. Start to heat up after 1 day, reach the highest temperature of 68℃ after 2 days, maintain the highest temperature for 9 days, and then ferment for 13 days. Turn the pile once every 2 days. The total fermentation time is 25 days.

[0054] Example 2: Treatment of bacterial residue in brewing wastewater using the method of the present invention

[0055] The specific steps are as follows:

[0056] a. Lactobacillus and Bacillus were cultured in liquid beef extract peptone medium until the bacterial concentration reached 10 8 / mL, Aspergillus and Trichoderma were cultured in potato sucrose medium respectively until the spore concentration reached 10 8 / mL;

[0057] The microbial agent raw materials are prepared, by weight, including 4 parts of Lactobacillus acidophilus, 1 part of Bacillus, 6 parts of Aspergillus niger, 5 parts of Trichoderma, 10 parts of barley grains, 22 parts of sorghum leaves, 10 parts of sugarcane leaves and 42 parts of water; and the mixture is cultured at room temperature for 15 days to prepare a microbial agent.

[0058] b. Add 16.7 kg of microbial agent and 0.67 kg of auxiliary agent to 1000 kg of brewing wastewater residue and mix well;

[0059] The auxiliary agent comprises: by weight, 68 parts of calcium sulfate and 32 parts of urea;

[0060] c. Ferment the mixed materials. Start to heat up after 1 day. The temperature reaches the highest temperature of 69°C after 2 days. Maintain the highest temperature for 10 days. Ferment for another 11 days. Turn the pile every 4 days. The total fermentation time is 24 days.

[0061] Example 3: Treatment of bacterial residue in brewing wastewater using the method of the present invention

[0062] The specific steps are as follows:

[0063] a. Lactobacillus and Bacillus were cultured in liquid beef extract peptone medium until the bacterial concentration reached 10 8 / mL, Aspergillus and Trichoderma were cultured in potato sucrose medium respectively until the spore concentration reached 10 8 / mL;

[0064] The microbial agent raw materials are prepared, by weight, including 5 parts of Lactobacillus acidophilus, 2 parts of Bacillus, 4 parts of Aspergillus niger, 4 parts of Trichoderma, 13 parts of barley grains, 19 parts of sorghum leaves, 8 parts of sugarcane leaves and 45 parts of water; and the mixture is cultured at room temperature for 16 days to prepare a microbial agent.

[0065] b. Add 10 kg of microbial agent and 0.56 kg of auxiliary agent to 1000 kg of brewing wastewater residue and mix well;

[0066] The auxiliary agent comprises: by weight, 75 parts of calcium sulfate and 25 parts of urea;

[0067] c. Ferment the mixed materials. Start to heat up after 1 day, reach the highest temperature of 71°C after 2 days, maintain the highest temperature for 11 days, and then ferment for 10 days. Turn the pile every 2 days, and the total fermentation time is 24 days.

[0068] Example 4: Treatment of bacterial residue in brewing wastewater using the method of the present invention

[0069] The specific steps are as follows:

[0070] a. Lactobacillus and Bacillus were cultured in liquid beef extract peptone medium until the bacterial concentration reached 10 8 / mL, Aspergillus and Trichoderma were cultured in potato sucrose medium respectively until the spore concentration reached 10 8 / mL;

[0071] The microbial agent raw materials are prepared, by weight, including 5 parts of Lactobacillus acidophilus, 3 parts of Bacillus, 6 parts of Aspergillus niger, 3 parts of Trichoderma, 10 parts of barley grains, 20 parts of sorghum leaves, 13 parts of sugarcane leaves and 40 parts of water; and the mixture is cultured at room temperature for 14 days to prepare a microbial agent.

[0072] b. Add 12.5 kg of microbial agent and 1 kg of auxiliary agent to 1000 kg of brewing wastewater residue and mix well;

[0073] The auxiliary agent comprises: by weight, 70 parts of calcium sulfate and 30 parts of urea;

[0074] c. Ferment the mixed materials. Start to heat up after 1 day, reach the highest temperature of 73°C after 2 days, maintain the highest temperature for 10 days, and ferment for another 10 days. Turn the pile every 3 days, and the total fermentation time is 23 days.

[0075] Comparative Example 5: The method of the present invention is not used to treat the bacterial residue in brewing wastewater

[0076] The specific steps are as follows:

[0077] a. Lactobacillus and Bacillus were cultured in liquid beef extract peptone medium until the bacterial concentration reached 10 8 / mL, Aspergillus and Trichoderma were cultured in potato sucrose medium respectively until the spore concentration reached 10 8 / mL;

[0078] Prepare the microbial agent raw materials, which include, by weight, 1 part of Lactobacillus acidophilus, 6 parts of Bacillus, 2 parts of Aspergillus niger, 1 part of Trichoderma, 20 parts of barley grains, 30 parts of sugarcane leaves and 40 parts of water; and culture at room temperature for 15 days to prepare the microbial agent.

[0079] b. Add 16.7 kg of microbial agent and 0.67 kg of auxiliary agent to 1000 kg of brewing wastewater residue and mix well;

[0080] The auxiliary agent comprises: by weight, 68 parts of calcium sulfate and 32 parts of urea;

[0081] c. Ferment the mixed material. Start to heat up after 2 days. The temperature reaches the highest temperature of 55℃ after 3 days. Maintain the highest temperature for 8 days. Ferment for another 11 days. Turn the pile every 2 days. The total fermentation time is 24 days.

[0082] Comparative Example 6: The method of the present invention is not used to treat the bacterial residue in brewing wastewater

[0083] The specific steps are as follows:

[0084] a. Lactobacillus and Bacillus were cultured in liquid beef extract peptone medium until the bacterial concentration reached 10 8 / mL, Aspergillus and Trichoderma were cultured in potato sucrose medium respectively until the spore concentration reached 10 8 / mL;

[0085] The microbial agent raw materials are prepared, by weight, including 8 parts of Lactobacillus acidophilus, 8 parts of Aspergillus niger, 10 parts of barley grains, 22 parts of sorghum leaves, 10 parts of sugarcane leaves and 42 parts of water; and the mixture is cultured at room temperature for 15 days to prepare the microbial agent.

[0086] b. Add 16.7 kg of microbial agent and 0.67 kg of auxiliary agent to 1000 kg of brewing wastewater residue and mix well;

[0087] The auxiliary agent comprises: by weight, 68 parts of calcium sulfate and 32 parts of urea;

[0088] c. Ferment the mixed material. Start to heat up after 2 days. The temperature reaches the highest temperature of 53°C after 3 days. Maintain the highest temperature for 9 days. Ferment for another 13 days. Turn the pile every 2 days. The total fermentation time is 25 days.

[0089] Comparative Example 7: The method of the present invention is not used to treat the bacterial residue in brewing wastewater

[0090] The specific steps are as follows:

[0091] a. Lactobacillus and Bacillus were cultured in liquid beef extract peptone medium until the bacterial concentration reached 10 8 / mL, Aspergillus and Trichoderma were cultured in potato sucrose medium respectively until the spore concentration reached 10 8 / mL;

[0092] The invention provides microbial agent raw materials, which include, by weight, 3 parts of Lactobacillus acidophilus, 3 parts of Bacillus, 7 parts of Aspergillus niger, 4 parts of Trichoderma, 12 parts of barley grains, 18 parts of sorghum leaves, 15 parts of sugarcane leaves and 38 parts of water; and prepares the microbial agent without culturing.

[0093] b. Add 20 kg of microbial agent and 1.25 kg of auxiliary agent to 1000 kg of brewing wastewater residue and mix well;

[0094] The auxiliary agent comprises: by weight, 60 parts of calcium sulfate and 40 parts of urea;

[0095] c. Ferment the mixed material. Start to heat up after 3 days. The temperature reaches the highest temperature of 48°C after 4 days. Maintain the highest temperature for 7 days. Ferment for another 13 days. Turn the pile over every 4 days. The total fermentation time is 26 days.

[0096] The fermented products of the examples and comparative examples were analyzed, and the results shown in Tables 1 to 3 below were obtained.

[0097] Table 1 Related indicators of bacterial residue in brewing wastewater after treatment by different methods

[0098]

[0099] Table 2 Heavy metal contents in bacterial residues in brewing wastewater after treatment by different methods

[0100] Item (mg / kg) Example 1 Example 2 Example 3 Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Total arsenic (As) 1.48 1.55 1.49 1.54 1.46 1.54 1.41 Total mercury (Hg) 0.04 0.04 0.04 0.04 0.03 0.03 0.04 Total lead (Pb) 23.40 25.36 22.28 23.64 22.49 23.54 24.18 Total cadmium (Cd) 0.39 0.40 0.38 0.38 0.31 0.36 0.39 Total chromium (Cr) 34.60 35.21 35.60 35.11 34.52 35.23 34.24

[0101] Table 3 Harmful microorganism content in bacterial residue in brewing wastewater after treatment by different methods

[0102]

[0103]

[0104] It can be seen from the results of the embodiments and comparative examples that the method of the present invention is used to ferment the bacterial residue in brewing wastewater, the fermentation time is short, the high temperature lasts for a long time, no odor is generated during the fermentation process, and the moisture content of the bacterial residue in the brewing wastewater can be effectively reduced, which is convenient for transportation and storage and reduces environmental pressure.

Claims

1. A method for treating brewing wastewater residue using microbial agents, characterized in that: The following steps are involved: a. Activate Lactobacillus, Bacillus, Aspergillus and Trichoderma, add barley grains, sorghum leaves, sugarcane leaves and water, and culture at room temperature for 10 to 20 days to prepare a microbial agent; b. Add the microbial agent and adjuvant obtained in step a to the brewing wastewater residue and mix well; c. Ferment the mixed material, start heating after 1 to 3 days, reach the highest temperature of 62 to 73°C after 2 to 7 days, maintain the highest temperature for 7 to 15 days, and then ferment for 10 to 13 days. The total fermentation time is 20 to 38 days; The lactobacillus described in step a is Lactobacillus acidophilus; the Bacillus is a mixture of Bacillus subtilis and Bacillus licheniformis in a weight ratio of 1-4:1-4; the Aspergillus is Aspergillus niger; and the Trichoderma is a mixture of Trichoderma harzianum and Trichoderma viride in a weight ratio of 1-8:1-8; The microbial agent described in step a comprises, by weight, 2-8 parts of Lactobacillus, 1-5 parts of Bacillus, 4-10 parts of Aspergillus, 2-6 parts of Trichoderma, 5-15 parts of barley grains, 10-28 parts of sorghum leaves, 8-18 parts of sugarcane leaves, and 30-50 parts of water; Among them, Aspergillus produces organic acid to inhibit the growth of miscellaneous bacteria, Trichoderma degrades cellulose in the residue mixture by secreting cellulase, Lactobacillus and Bacillus decompose starch, sugar and protein substances, barley grains provide necessary nutrients for the composite microorganisms, sugarcane leaves and sorghum leaves serve as adsorption and coating carriers, providing sufficient growth space for effective bacteria, promoting the growth of effective live bacteria and improving the survival rate. Sugarcane leaves and sorghum leaves serve as loosening agents in the fermentation process, increasing the oxygen supply for microbial growth, avoiding anaerobic fermentation and reducing the generation of odor.

2. The method according to claim 1, wherein: The activation of Lactobacillus and Bacillus in step a refers to culturing the cells in liquid beef extract peptone medium to a concentration of ≥10 8 / mL; the activation of Aspergillus and Trichoderma refers to the use of potato sucrose medium to culture to a spore concentration of ≥10 8 / mL.

3. The method according to claim 1, wherein: The composition of the microbial agent described in step a includes: by weight, 5 parts of lactobacillus, 3 parts of bacillus, 6 parts of aspergillus, 3 parts of trichoderma, 10 parts of barley grains, 20 parts of sorghum leaves, 13 parts of sugarcane leaves, and 40 parts of water.

4. The method according to claim 1, wherein: The particle size of the sorghum leaves and sugarcane leaves in step a is 3 to 8 cm.

5. The method according to claim 1, wherein: The auxiliary agent described in step b comprises: 60 to 90 parts by weight of calcium sulfate and 10 to 40 parts of urea.

6. The method according to claim 1, wherein: The weight ratio of the microbial agent to the bacterial residue in step b is 1:50-100.

7. The method according to claim 1, wherein: The weight ratio of the auxiliary agent described in step b to the fungus residue is 1:500-2000.

8. The method according to claim 1, wherein: The fermentation conditions in step c are as follows: the temperature starts to rise after 1 day, reaches a maximum temperature of 73° C. after 2 days, maintains the maximum temperature for 10 days, and then ferments for another 10 days, with a total fermentation time of 23 days.

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