A system, method and application for rapid analysis of algae concentration in algae-containing water bodies

Abstract

The present invention belongs to the technical field of water body analysis, and discloses a system, method and application for rapid analysis of algae concentration in algae-containing water bodies; a power supply and a microprocessor are embedded on the inner wall of a closed shell, a CCD camera is fixed below the power supply, an optical path is connected to the CCD camera below through a first threaded interface, an objective lens is connected to the optical path below through a second threaded interface, and a circle of magnetic rings is embedded at the bottom of the lower end of the closed shell; two ends of the magnetic ring are each connected to a retractable rod; a magnet is connected below the retractable rod, a groove is fixedly connected to the middle position of the magnet, and a counting groove is arranged inside the groove; a magnet is fixedly connected to the outer ring of the counting groove, and a drain port is provided on the contact surface of the upper surface of the counting groove with the magnet; an LED surface light source is fixed below the groove, and a light source driving device is connected to one side of the LED surface light source; a hook is connected to the outer wall of the closed shell. The present invention has more standardized control for measuring water samples, fast measuring speed, high efficiency and accurate results.

Description

Technical Field

[0001] The present invention belongs to the technical field of water body analysis, and in particular relates to a system, method and application for rapid analysis of algae concentration in algae-containing water bodies. Background Art

[0002] At present, the closest prior art is: due to eutrophication of water bodies, some lakes and reservoirs and other water sources are multiplied in large quantities, wherein, water bloom blue algae can be synthesized in the growth process and release harmful metabolites such as algae toxins, dimethyl isoborneol, and geosmell to the water body, these harmful substances will not only affect the sensory properties of water, but also produce huge toxic effects on fish and shrimp and even human and livestock, seriously threatening water supply safety. Algae has been widely used as an indicator organism for major ecological and environmental problems such as global climate change, eutrophication of lake water bodies, and marine disasters (marine ecosystem structure degradation, functional area damage, red tide, etc.), and plays an important role in ecological and environmental monitoring. In addition, driven by conditions such as meteorology, hydraulics, and water environment factors, the abnormal proliferation of algae may seriously damage the health of the water environment. Therefore, the rapid analysis of algae concentration has important guiding significance for the supervision and protection of the water ecological environment, and is to take measures to control in time, and to prevent the abnormal proliferation of algae from gaining valuable time.

[0003] Traditional methods for analyzing algae concentration mainly include microscopic photography and counting and chlorophyll content determination. Microscopic photography is labor-intensive and inefficient, and this method requires sampling first and bringing the samples back. Professionals are required to image the samples under a microscope and then count them with the naked eye, which is time-consuming and laborious. Chlorophyll content determination mainly involves on-site sampling, bringing them back to the laboratory, and then analyzing them with a UV / fluorescence spectrophotometer to obtain the absorbance / fluorescence intensity of the samples. The sample concentration is indirectly obtained based on the proportional relationship between the absorbance / fluorescence intensity and the concentration. With the development of science and technology, satellite remote sensing monitoring has gradually been applied to the analysis of large-scale field algae concentrations. In remote sensing monitoring, the chlorophyll concentration in the water body is inverted through high-resolution satellite images and spectral characteristics. This method is easily affected by remote sensing resolution and weather, and remote sensing can only be used to monitor surface algae blooms.

[0004] In summary, the problems existing in the prior art are:

[0005] (1) Microscopic photography counting and analysis method: This method is inefficient, the results are inaccurate, the labor intensity is high, and the long-term observation is very harmful to the eyes of the counting personnel.

[0006] (2) Chlorophyll content determination and analysis method: sampling and then making a standard curve, and comparing the UV absorbance / fluorescence intensity with the standard curve to obtain the concentration, which is time-consuming and labor-intensive.

[0007] (3) Satellite remote sensing monitoring and analysis method: The chlorophyll concentration in the water body is inverted through high-resolution satellite images and spectral characteristics. It is easily affected by weather and can only be used to monitor surface algal blooms.

[0008] The difficulty of solving the above technical problems: how to improve the detection and analysis methods, improve the accuracy of the detection results, and reduce the workload.

[0009] The significance of solving the above technical problems: Solving the above technical problems has important guiding significance for the supervision and protection of the water ecological environment, and buys precious time for taking timely measures to prevent abnormal proliferation of algae. Summary of the invention

[0010] In view of the problems existing in the prior art, the present invention provides a system, method and application for quickly analyzing the concentration of algae in algae-containing water bodies.

[0011] The present invention is implemented as follows: a system for rapidly analyzing the concentration of algae in algae-containing water bodies, wherein the system for rapidly analyzing the concentration of algae in algae-containing water bodies is provided with:

[0012] Close the housing;

[0013] A power supply and a microprocessor are embedded on the inner wall of the closed shell, a CCD camera is fixed below the power supply, an optical path is connected to the CCD camera below through a first threaded interface, and an objective lens is connected to the optical path below through a second threaded interface, and a circle of magnetic rings is embedded in the bottom of the lower end of the closed shell; both ends of the magnetic ring are connected to a retractable rod; a magnet is connected below the retractable rod, a groove is fixedly connected to the middle position of the magnet, and an LED surface light source is fixed below the groove.

[0014] Furthermore, an LED surface light source is arranged below the groove, a counting slot is arranged inside the groove, and a light source driving device is connected to one side of the LED surface light source;

[0015] The outer ring of the counting groove is fixedly connected with a magnet, and the upper surface of the counting groove in contact with the magnet is provided with a drainage port.

[0016] Furthermore, the light source driving device is connected to the LED surface light source through a wire.

[0017] Furthermore, the power supply is connected to the microprocessor and the CCD camera through wires, the microprocessor is connected to the CCD camera through wires, and the light source driving device is connected to the LED surface light source through wires.

[0018] Another object of the present invention is to provide a method for rapidly analyzing the algae concentration in algae-containing water bodies by executing the system for rapidly analyzing the algae concentration in algae-containing water bodies. The method for rapidly analyzing the algae concentration in algae-containing water bodies comprises: placing algae in water bodies with different concentrations, the water in the water bodies enters the counting groove of the groove, lifting the device and placing it vertically, the excess water sample in the counting groove will flow out of the device through the drain port, turning on the LED surface light source through the light source driving device, the LED light source irradiates the water sample, the water sample image is formed by the objective lens and the optical path in the CCD camera, the image is counted by the noise reduction - contour extraction - blackness analysis - contour statistics, and then the naked algae concentration map per unit volume is obtained according to the volume quantitative principle of the device used.

[0019] Another object of the present invention is to provide an intelligent terminal using the algae concentration rapid analysis system for algae-containing water bodies.

[0020] Another object of the present invention is to provide an application of the rapid analysis system for algae concentration in algae-containing water bodies in the supervision and protection of aquatic ecological environment.

[0021] Another object of the present invention is to provide a water body analysis information processing system using the algae concentration rapid analysis system for algae-containing water bodies.

[0022] Another object of the present invention is to provide an application of the algae concentration rapid analysis system for algae-containing water bodies in algae concentration analysis.

[0023] In summary, the advantages and positive effects of the present invention are as follows: the counting slot provided in the present invention can control the volume of the sample to be tested. The drainage slot provided in the present invention can ensure the consistency of the volume of the sample to be tested and maintain the stability of the measurement. The present invention controls the CCD camera imaging through the light source driving device and the microprocessor.

[0024] The invention has a simple structure and adopts the physical amplification imaging principle to measure the algae concentration. Compared with the traditional sampling and standard curve comparison analysis which takes 1-2 days, the system does not need sampling. The algae concentration rapid analysis system of algae-containing water body is directly placed in the water body. The concentration of algae in the water body can be obtained within 1 hour. The water sample control is more standardized, the measurement speed is fast, the efficiency is high, the result is more accurate, the labor intensity is low, and the supervision of the water ecological environment and timely measures to prevent abnormal proliferation of algae can buy precious time. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Schematic diagram of the structure of a system for rapidly analyzing algae concentration in algae-containing water provided by an embodiment of the present invention;

[0026] Figure 2 is a schematic diagram of the drain outlet structure provided by an embodiment of the present invention;

[0027] Figure 3 is a schematic diagram of the rapid analysis result of algae concentration provided by an embodiment of the present invention;

[0028] In the figure: 1. housing; 2. groove; 3. power supply; 4. microprocessor; 5. CCD camera; 6. first threaded interface; 7. optical path; 8. second threaded interface; 9. objective lens; 10. LED light source; 11. magnetic ring; 12. magnet; 13. counting slot; 14. drain outlet; 15. light source driving device; 16. telescopic rod; 17. hook. DETAILED DESCRIPTION

[0029] In order to further understand the content, features and effects of the present invention, the following embodiments are given as examples and described in detail with reference to the accompanying drawings.

[0030] In view of the problems existing in the prior art, the present invention provides a system, method and application for rapid analysis of algae concentration in algae-containing water bodies. The present invention is described in detail below in conjunction with the accompanying drawings.

[0031] like Figure 1-Figure 2 As shown, the algae concentration rapid analysis system of algae-containing water provided by the embodiment of the present invention includes: a housing 1, a groove 2, a power supply 3, a microprocessor 4, a CCD camera 5, a first threaded interface 6, an optical path 7, a second threaded interface 8, an objective lens 9, an LED light source 10, a magnetic ring 11, a magnet 12, a counting slot 13, a drain outlet 14, a light source driving device 15, a retractable rod 16, and a hook 17.

[0032] A power supply 3 and a microprocessor 4 are embedded on the inner wall of the closed shell 1, a CCD camera 5 is fixed below the light source driving device 15, an optical path 7 is connected to the bottom of the CCD camera 5 through a first threaded interface 6, and an objective lens 9 is connected to the bottom of the optical path 7 through a second threaded interface 8. A circle of magnetic rings 11 is embedded in the bottom of the lower end of the closed shell 1; a retractable rod 16 is connected to each of the two ends of the magnetic ring; a magnet 12 is connected to the bottom of the retractable rod, and a groove 2 is fixedly connected to the middle position of the magnet.

[0033] An LED surface light source 10 is disposed below the groove 2 , a counting slot 13 is disposed inside the groove 2 , and a light source driving device 15 is connected to one side of the LED surface light source 10 .

[0034] The outer ring of the counting groove 13 is fixedly connected with a magnet 12 , and the upper surface of the counting groove 13 in contact with the magnet 12 is provided with a drainage port 14 .

[0035] The magnet 12 fixed on the outer ring of the counting groove 13 can be attracted to the magnetic ring 11 fixed on the closed shell 1, and the sample in the counting groove 13 is in focus; the drainage port 14 set on the contact surface between the upper surface of the counting groove 13 and the magnet 12 can drain the excess sample liquid in the counting groove 13 out of the groove.

[0036] The power supply 3 is connected to the microprocessor 4 and the CCD camera 5 through wires, the microprocessor 4 is connected to the CCD camera 5 through wires, and the light source driving device 15 is connected to the LED surface light source 10 through wires. The light source driving device 15 and the microprocessor 4 control the CCD camera imaging.

[0037] In a preferred embodiment of the present invention, the microprocessor model is MCIMX6L2EVN10AB.

[0038] In a preferred embodiment of the present invention, the centers of the CCD camera 5, the optical path 7, and the objective lens 9 are located on the same vertical line.

[0039] In a preferred embodiment of the present invention, the waterproof LED surface light source 10 is a standard annular light source.

[0040] In a preferred embodiment of the present invention, the center line of the waterproof LED surface light source 10 coincides with the center line of the objective lens 9 .

[0041] In a preferred embodiment of the present invention, the light source driving device 15 is composed of a pulse width modulation circuit.

[0042] In a preferred embodiment of the present invention, the counting slot 13 is made of a special glass slide.

[0043] In a preferred embodiment of the present invention, the volume of the counting tank 13 is 2 μL.

[0044] When the present invention is used, the device is placed in a body of water with different algae concentrations, and the water in the body of water enters the counting groove 13 of the groove 2. The device is lifted and placed vertically, and the excess water sample in the counting groove 13 will flow out of the device through the drain port 14. The LED surface light source 10 is turned on by the light source driving device, and the LED light source 10 irradiates the water sample. The water sample image is imaged by the objective lens 9 and the optical path 7 on the CCD camera 5. The imaged picture is processed by noise reduction-contour extraction-blackness analysis-contour statistics to obtain the average number N of samples, and then the algae concentration map per unit volume is obtained according to the volume quantitative principle of the device used in the present invention, as shown in FIG. Figure 3 shown.

[0045] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Any simple modification, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention are within the scope of the technical solution of the present invention.

Claims

1. A rapid analysis system for algae concentration in algae-containing water bodies, It is characterized in that The algae concentration rapid analysis system of algae-containing water body is provided with: Close the housing; A power supply and a microprocessor are embedded on the inner wall of the closed housing, a CCD camera is fixed below the power supply, an optical path is connected to the CCD camera through a first threaded interface, and an objective lens is connected to the optical path through a second threaded interface. A magnetic ring is embedded at the bottom of the lower end of the closed housing; two ends of the magnetic ring are respectively connected to a retractable rod; a magnet is connected to the lower part of the retractable rod, and a groove is fixedly connected to the middle of the magnet; An LED surface light source is arranged below the groove, a counting slot is arranged inside the groove, and a light source driving device is connected to one side of the LED surface light source; The outer ring of the counting slot is fixedly connected with a magnet, and the upper surface of the counting slot in contact with the magnet is provided with a drainage port; The drainage port is arranged on the contact surface between the upper surface of the counting tank and the magnet to discharge the excess sample liquid in the counting tank out of the tank.

2. The algae concentration rapid analysis system of algae-containing water body according to claim 1, It is characterized in that The light source driving device is connected to the LED surface light source through a wire.

3. The algae concentration rapid analysis system of algae-containing water body according to claim 1, It is characterized in that The power supply is connected to the microprocessor and the CCD camera through wires, the microprocessor is connected to the CCD camera through wires, and the light source driving device is connected to the LED surface light source through wires.

4. The algae concentration rapid analysis system of algae-containing water body according to claim 1, It is characterized in that A hook is connected to the outer wall of the closed shell.

5. A method for rapidly analyzing algae concentration in algae-containing water body using the system for rapidly analyzing algae concentration in algae-containing water body according to any one of claims 1 to 3, It is characterized in that The method for rapidly analyzing the algae concentration of algae-containing water bodies comprises: placing algae with different concentrations into water bodies, allowing the water in the water bodies to enter the counting groove of the groove, lifting the device and placing it vertically, allowing the excess water in the counting groove to flow out of the device through the drain port, turning on the LED surface light source through a light source driving device, irradiating the water sample with the LED light source, imaging the water sample image on a CCD camera through an objective lens and an optical path, and counting the image through noise reduction-contour extraction-blackness analysis-contour statistics to obtain an average number N of samples, and then obtaining an algae concentration map per unit volume according to the volume quantitative principle of the device used.

6. An intelligent terminal using the system for rapid analysis of algae concentration in algae-containing water bodies as described in any one of claims 1 to 3.

7. Use of the rapid analysis system for algae concentration in algae-containing water as claimed in any one of claims 1 to 3 in the supervision and protection of aquatic ecological environment.

8. A water body analysis information processing system using the algae concentration rapid analysis system for algae-containing water bodies as described in any one of claims 1 to 3.

9. Use of the system for rapid analysis of algae concentration in algae-containing water as claimed in any one of claims 1 to 3 in analysis of algae concentration.

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