Liver cancer cell proliferation observation device

Abstract

The invention discloses a device for observing the proliferation of liver cancer cells, comprising an inverted microscope device body, a base, an electric control switch button, a socket, a support platform, a control panel, a stage lifting button, a stage rotation button, an objective lens rotation button, an objective lens lifting button, an objective lens device, an objective lens, a first rotating shaft, a first rotating wheel, a belt, a second rotating wheel, a first motor, a shell, a connecting rod, a sliding rod, a groove wheel, a second rotating shaft, a regulator, a second motor, an eyepiece, a camera, a liquid crystal display screen, a third rotating shaft, a support frame, a light source, a stage device, a rotating table, a gear, a third motor, an automatic lifting device, a fourth motor, a screw rod, a telescopic rod and a stage. The advantages of the invention are: the machine base is integrated and ergonomic; a living cell culture dish can be directly placed on the stage to monitor the cell proliferation state in real time; the lifting and rotation of the stage and the objective lens device can be electrically controlled to solve the tedious work and errors caused by manual experiments.

Description

Technical Field

[0001] The invention relates to a liver cancer cell proliferation observation device, belonging to the technical field of medical equipment. Background Art

[0002] Liver cancer is a malignant tumor of the liver, which can be divided into two categories: primary and secondary. Primary malignant liver tumors originate from the epithelial or mesenchymal tissue of the liver. The former is called primary liver cancer, which is a highly prevalent and extremely harmful malignant tumor in my country; the latter is called sarcoma, which is relatively rare compared with primary liver cancer. Secondary or metastatic liver cancer refers to malignant tumors originating from multiple organs throughout the body that invade the liver. It is generally common in liver metastases from malignant tumors of organs such as the stomach, bile duct, pancreas, colorectum, ovary, uterus, lung, and breast.

[0003] The biological background of liver cancer varies greatly, and the prognosis and corresponding treatment of different liver cancers also vary greatly. In order to reflect the differences between tumors, evaluate the prognosis and treat tumors, multiple studies have conducted molecular classification of liver cancer. One study analyzed the whole genome, transcriptome and epigenetic map of liver cancer and divided liver cancer into two categories, namely proliferative and non-proliferative, and each type accounts for about 50% of liver cancer.

[0004] Proliferative liver cancer is mainly characterized by the activation of signaling pathways related to cell proliferation and survival, including PI3K-AKT-mTOR, RAS-MAPK and MET signaling cascade signaling pathways, among which TP53 inactivation, FGF19 and CCND1 amplification are significantly enriched. Proliferative liver cancer can be divided into two subtypes, WNT-TGFβ type (S1 type) and progenitor cell group (S2 type). The former is mainly characterized by non-classical activation of WNT signaling, and the latter is mainly characterized by overexpression of EPCAM, AFP and IGF2 and high DNA methylation. How to effectively inhibit the proliferation ability of liver cancer cells and promote the apoptosis of liver cancer cells is the key to the treatment of liver cancer. The current observation of liver cancer cell proliferation is all carried out under an inverted microscope. The stage and objective lens converter of the existing inverted microscope are mostly manually adjusted, and the microscope needs to be connected to an external computer, which is more troublesome to operate and is a problem that needs to be solved. Summary of the invention

[0005] The purpose of the present invention is to provide a device for observing liver cancer cell proliferation.

[0006] The problem to be solved by the present invention is that the stage and the objective lens converter on the inverted microscope in the existing liver cancer cell proliferation observation device are mostly manually adjusted, and the inverted microscope needs to be connected to an external computer.

[0007] To achieve the purpose of the present invention, the technical solution adopted by the present invention is:

[0008] The invention discloses a device for observing the proliferation of liver cancer cells, comprising an inverted microscope device body, a base, an electric control switch button, a socket, a support platform, a control panel, a stage lifting button, a stage rotation button, an objective lens rotation button, an objective lens lifting button, an objective lens device, an objective lens, a first rotating shaft, a first rotating wheel, a belt, a second rotating wheel, a first motor, a shell, a connecting rod, a sliding rod, a groove wheel, a second rotating shaft, a regulator, a second motor, an eyepiece, a camera, a liquid crystal display, a third rotating shaft, a support frame, a light source, a stage device, a rotating table, a gear, a third motor, an automatic lifting device, a fourth motor, a screw, a telescopic rod and a stage. The inverted microscope device body is provided with a base, an electric control switch button is provided on the right side surface of the base, a socket is provided on the back side of the base, a support platform is provided on the base, a control panel is provided on the right side surface of the support platform, a stage lifting button is provided in the control panel, a stage rotation button is provided at the lower end of the stage lifting button, an objective lens rotation button is provided at the left side of the stage rotation button, an objective lens lifting button is provided at the upper end of the objective lens rotation button, and a control panel is provided. The panel wire is connected to the objective lens device, the objective lens is installed on the objective lens device, a first rotating shaft is installed under the objective lens, a first rotating wheel is installed on the first rotating shaft, a belt is installed on the first rotating wheel, a second rotating wheel is installed on the left side of the belt, a first motor is installed on the second rotating wheel, a shell is installed outside the first motor, a connecting rod is installed outside the shell, a sliding rod is installed on the connecting rod, a groove wheel is installed at the end of the sliding rod, a second rotating shaft is installed at the center of the groove wheel, an adjuster is installed on the left side of the second rotating shaft, a second motor is installed on the right side of the second rotating shaft, an eyepiece is installed on the support platform, a camera is installed on the eyepiece, a data cable of the camera is connected to the LCD screen, a third rotating shaft is installed at the bottom of the LCD screen, a support frame is installed under the third rotating shaft, a light source is arranged on the support frame, a stage device is installed under the light source, a rotating stage is installed on the stage device, a gear is installed on the rotating stage, the gear and the third motor are twisted, an automatic lifting device is installed under the stage, a fourth motor is installed in the automatic lifting device, the fourth motor is connected to a screw, a telescopic rod is installed outside the screw, and a stage is fixedly installed on the top of the telescopic rod.

[0009] Furthermore, the LCD screen is a high-definition LCD screen, the image color restoration is close to the original color, USB synchronous output is possible, and an SD card can be used to take pictures or record videos.

[0010] Furthermore, the stage lifting button can select three modes of "raise, lower, and close", the stage rotation button can select three modes of "counterclockwise, clockwise, and close", the objective lens rotation button can select three modes of "counterclockwise, clockwise, and close", and the objective lens lifting button can select three modes of "raise, lower, and close".

[0011] The advantages of the present invention are: the machine base is integrated and ergonomic; the living cell culture dish can be directly placed on the stage to monitor the cell proliferation state in real time; the lifting and rotation of the stage and the objective lens device can be electrically controlled to solve the tedious work and errors caused by manual experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the structure of a liver cancer cell proliferation observation device;

[0013] Figure 2 It is a schematic diagram of the structure of the objective lens device of the liver cancer cell proliferation observation device;

[0014] Figure 3 It is a schematic diagram of the structure of a stage device of a liver cancer cell proliferation observation device;

[0015] In the figure: 1, inverted microscope device body 2, base 3, electric control switch button 4, socket 5, support platform 6, control panel 61, stage lifting button 62, stage rotation button 63, objective lens rotation button 64, objective lens lifting button 7, objective lens device 71, objective lens 72, first rotating shaft 73, first rotating wheel 74, belt 75, second rotating wheel 76, first motor 77, housing 78, connecting rod 79, sliding rod 710, groove wheel 711, second rotating shaft 712, regulator 713, second motor 8, eyepiece 9, camera 10, liquid crystal display screen 11, third rotating shaft 12, support frame 13, light source 14, stage device 141, rotating table 142, gear 143, third motor 144, automatic lifting device 145, fourth motor 146, screw 147, telescopic rod 148, stage. DETAILED DESCRIPTION

[0016] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0017] The liver cancer cell proliferation observation device includes an inverted microscope device body 1, a base 2, an electric control switch button 3, a socket 4, a support platform 5, a control panel 6, a stage lifting button 61, a stage rotation button 62, an objective lens rotation button 63, an objective lens lifting button 64, an objective lens device 7, an objective lens 71, a first rotating shaft 72, a first rotating wheel 73, a belt 74, a second rotating wheel 75, a first motor 76, a housing 77, a connecting rod 78, a sliding rod 79, a groove wheel 710, a second rotating shaft 711, an adjuster 712, a second motor 713, an eyepiece 8, a camera 9, a liquid crystal display screen 10, a third rotating shaft 11, a support frame 12, a light source 13, a stage device 14, a rotating table 141, a gear 142, a third motor 143, and an automatic lifting device. 144, a fourth motor 145, a screw rod 146, a telescopic rod 147, and a stage 148. A base 2 is installed on the body 1 of the inverted microscope device for providing support. An electric control switch button 3 is installed on the right side of the base 2 for connecting or cutting off the power supply. A socket 4 is installed on the back of the base 2 for connecting the power supply. A support platform 5 is installed on the base 2 for providing support. A control panel 6 is installed on the right side of the support platform 5 for providing signal input. A stage lifting button 61 is installed in the control panel 6 for providing a "lifting" signal input. A stage rotation button 62 is installed at the lower end of the stage lifting button 61 for providing a "rotation" signal input. An objective lens rotation button 63 is installed on the left side of the stage rotation button 62 for providing a "rotation" signal input. " signal input, an objective lens lifting button 64 is installed on the upper end of the objective lens rotation button 63 for providing a "lifting" signal input, the control panel 6 is connected to the objective lens device 7 by wires for approaching the observation object, an objective lens 71 is installed on the objective lens device 7 for approaching the observation object, a first rotating shaft 72 is installed under the objective lens 71 for providing rotation, a first rotating wheel 73 is installed on the first rotating shaft 72 for providing rotation, a belt 74 is installed on the first rotating wheel 73 for transmitting power, a second rotating wheel 75 is installed on the left side of the belt 74 for providing rotation, a first motor 76 is installed on the second rotating wheel 75 for providing power, a shell 77 is installed outside the first motor 76 for providing protection, a connecting rod 78 is installed outside the shell 77 for connecting components, and the connecting rod 78 is installed outside the shell 77. A slide bar 79 is installed on 8 for sliding, a groove wheel 710 is installed at the end of the slide bar 79 for rotation, a second shaft 711 is installed at the center of the groove wheel 710 for rotation, an adjuster 712 is installed on the left side of the second shaft 711 for adjusting the lifting, a second motor 713 is installed on the right side of the second shaft 711 for power, an eyepiece 8 is installed on the support platform 5 for observing the object, a camera 9 is installed on the eyepiece 8 for photographing the object, the camera 9 is connected to the LCD screen 10 with a data cable for displaying the picture, a third shaft 11 is installed at the bottom of the LCD screen 10 for rotation, a support frame 12 is installed under the third shaft 11 for support, and a light source 13 is provided on the support frame 12 for providing light source.A stage device 14 is installed under the light source 13 for placing the observation object. A rotating platform 141 is installed on the stage device 14, which is a rotating component. A gear 142 is installed on the rotating platform 141 for transmitting power. The gear 142 is twisted with a third motor 143 for providing power. An automatic lifting device 144 is installed under the stage 141 for providing lifting. A fourth motor 145 is installed in the automatic lifting device 144 for providing power. The fourth motor 145 is connected to a screw rod 146 for transmitting power. A telescopic rod 147 is installed outside the screw rod 146 for providing telescopic. A stage 148 is fixedly installed on the top of the telescopic rod 147 for placing the observation object.

[0018] Furthermore, the LCD screen 10 is a high-definition LCD screen, the image color restoration is close to the original color, USB synchronous output is possible, and an SD card can be used to take pictures or record videos.

[0019] Furthermore, the stage lifting button 61 can select three modes of "raise, lower, and close", the stage rotation button 62 can select three modes of "counterclockwise, clockwise, and close", the objective lens rotation button 63 can select three modes of "counterclockwise, clockwise, and close", and the objective lens lifting button 64 can select three modes of "raise, lower, and close".

[0020] Usage: connect the socket 4 to the power supply, turn on the electric control switch button 3, place the object to be observed on the stage, press the objective lens rotation button 63, the first motor 76 starts to work, drives the first rotating shaft 72 to rotate, the first rotating shaft 72 rotates and the objective lens 71 rotates, when it rotates to the corresponding objective lens 71, close the objective lens rotation button 63, and the objective lens 71 stops rotating; press the objective lens lifting button 62, the second motor 713 starts to work, the second motor 713 drives the second rotating shaft 711 to rotate, the second rotating shaft 711 rotates and drives the groove wheel 710 to rotate, the groove wheel 710 rotates and drives the connecting rod 78 to move up and down, the connecting rod 78 drives the housing 77 and the objective lens 71 to rise and fall, when the objective lens 71 rises and falls to the appropriate position, close the objective lens lifting button 62; the regulator 712 can also be manually adjusted to make the objective lens 71 rise and fall to the appropriate position; after adjusting the light source 13, the objective lens 71 is raised and lowered through the eyepiece 8 Observe, adjust the stage device 14, and select the observation field of view; press the stage lifting button 61, the fourth motor 145 starts working, drives the screw 146 to rotate, the rotation of the screw 146 drives the nut in the automatic lifting device 144 to move, the movement of the nut drives the telescopic rod 147 to move up and down, the telescopic rod 147 moves up and down to drive the stage 148 to rise and fall, when the stage 148 is lifted to a suitable height, close the stage lifting button 61; press the stage rotation button 62, the third motor 143 starts working, the third motor 143 drives the gear 142 to rotate, the rotation of the gear 142 drives the rotating table 141 to rotate, when the rotating table 141 rotates to a suitable position, close the stage rotation button 62, and the rotating table 141 stops rotating; after the observation is completed, remove the observation object, push and pull the light source 13 to the darkest, turn off the electric control switch button 3, and unplug the socket 4 to disconnect the power.

Claims

1. A liver cancer cell proliferation observation device, comprising an inverted microscope device body (1), a base (2), an electric control switch button (3), a socket (4), a support table (5), a control panel (6), a stage lifting button (61), a stage rotation button (62), an objective lens rotation button (63), an objective lens lifting button (64), an objective lens device (7), an objective lens (71), a first rotating shaft (72), a first rotating wheel (73), a belt (74), a second rotating wheel (75), a first motor (76), a housing (77), and a connecting rod (78). ), a slide bar (79), a groove wheel 710, a second rotating shaft (711), an adjuster (712), a second motor (713), an eyepiece (8), a camera (9), a liquid crystal display screen (10), a third rotating shaft (11), a support frame (12), a light source (13), a stage device (14), a rotating table (141), a gear (142), a third motor (143), an automatic lifting device (144), a fourth motor (145), a screw rod (146), a telescopic rod (147), and a stage (148), wherein: The inverted microscope device body (1) is provided with a base (2), an electric control switch button (3) is provided on the right side surface of the base (2), a socket (4) is provided on the back side of the base (2), a support platform (5) is provided on the base (2), a control panel (6) is provided on the right side surface of the support platform (5), a stage lifting button (61) is provided in the control panel (6), a stage rotation button (62) is provided at the lower end of the stage lifting button (61), an objective lens rotation button (63) is provided on the left side of the stage rotation button (62), an objective lens lifting button (64) is provided at the upper end of the objective lens rotation button (63), a wire of the control panel (6) is connected to an objective lens device (7), an objective lens (71) is provided on the objective lens device (7), and an objective lens (71) is provided on the objective lens device (7). A first rotating shaft (72) is installed under the mirror (71), a first rotating wheel (73) is installed on the first rotating shaft (72), a belt (74) is installed on the first rotating wheel (73), a second rotating wheel (75) is installed on the left side of the belt (74), a first motor (76) is installed on the second rotating wheel (75), a housing (77) is installed outside the first motor (76), a connecting rod (78) is installed outside the housing (77), a sliding rod (79) is installed on the connecting rod (78), a groove wheel (710) is installed at the end of the sliding rod (79), a second rotating shaft (711) is installed at the center of the groove wheel (710), an adjuster (712) is installed on the left side of the second rotating shaft (711), and a second motor (710) is installed on the right side of the second rotating shaft (711). 3), an eyepiece (8) is mounted on the support platform (5), a camera (9) is mounted on the eyepiece (8), a data cable of the camera (9) is connected to a liquid crystal display screen (10), a third rotating shaft (11) is mounted on the bottom end of the liquid crystal display screen (10), a support frame (12) is mounted under the third rotating shaft (11), a light source (13) is mounted on the support frame (12), a loading platform device (14) is mounted under the light source (13), a rotating platform (141) is mounted on the loading platform device (14), a gear (142) is mounted on the rotating platform (141), the gear (142) is gear-twisted with a third motor (143), an automatic lifting device (144) is mounted under the loading rotating platform (141), and a third motor (143) is mounted in the automatic lifting device (144). Four motors (145), the fourth motor (145) is connected to a screw rod (146), a telescopic rod (147) is installed outside the screw rod (146), a loading platform (148) is fixedly installed on the top of the telescopic rod (147), the liquid crystal display screen (10) is a high-definition liquid crystal display screen, the image color restoration is close to the original color, USB synchronous output can be used, and an SD card can be used to take pictures or record videos, the loading platform lifting button (61) can select three modes of "raising, lowering, and closing", the loading platform rotation button (62) can select three modes of "counterclockwise, clockwise, and closing", the objective lens rotation button (63) can select three modes of "counterclockwise, clockwise, and closing", and the objective lens lifting button (64) can select three modes of "raising, lowering, and closing".

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