A saliva collection device with a disease-enriching adsorption structure

Abstract

The present invention proposes a saliva collection device with a virus enrichment and adsorption structure, comprising a sampler and a sample storage bottle; the sampler comprises a hollow package, a resin adsorption module and a negative pressure control device; the package is provided with a collection port and a negative pressure port; the resin adsorption module is placed in the package and is arranged at one end of the package close to the collection port; the negative pressure control device is detachably connected to the negative pressure port of the package; the sample storage bottle is preset with a sample preservative solution; after the sampler takes a sample, it is inserted into the sample storage bottle, so as to propose a saliva collection device that can achieve effective adsorption and enrichment for viruses and short-term storage of viruses.

Description

Technical Field

[0001] The invention relates to the technical field of biological preservation, and in particular to a saliva collection device with a disease enrichment adsorption structure. Background Art

[0002] Saliva is an important biological fluid and one of the commonly used test specimens in biomedical research and clinical practice. Under normal physiological conditions, in addition to oral epithelial cells shed from the donor's mouth, saliva also contains a variety of proteins / enzyme molecules, extracellular vesicles / exosomes, micronucleic acid molecules, and microbial cells of normal flora secreted from salivary glands. In disease states such as viral or bacterial infections, it may also contain viral particles, bacteria, or fungal pathogen cells. Studies have shown that saliva specimens are more stable and reliable than samples collected by other methods such as nasopharyngeal swabs for viral nucleic acid detection after respiratory viral infection. The various existing devices for saliva collection and sampling are simple and direct, without adsorption enrichment for the biological components to be detected, no non-toxic built-in sampling success mark, and no protection performance for related biological components; the sampling operation must also be completed by trained professionals. Therefore, the existing saliva sampling device cannot be completed by the general public themselves, nor can it be used for short-term storage of samples, so it cannot be used for remote delivery of saliva samples. Summary of the invention

[0003] The purpose of the present invention is to provide a saliva collection device that can effectively adsorb and enrich viruses and achieve short-term storage of viruses.

[0004] To achieve the above-mentioned purpose, the present invention provides a saliva collection device with a disease enrichment adsorption structure, comprising a sampler and a sample storage bottle;

[0005] The sampler comprises a hollow package, a resin adsorption module and a negative pressure control device; the package is provided with a collection port and a negative pressure port; the resin adsorption module is placed in the package and is arranged at one end of the package close to the collection port; the negative pressure control device is detachably connected to the negative pressure port of the package;

[0006] The sample storage bottle is preset with a sample preservative solution; after the sampler takes a sample, it is inserted into the sample storage bottle.

[0007] Preferably, the package is a hollow glass package, the package is a columnar structure, the head end of the package is the collection port, and the tail end of the package is the negative pressure port.

[0008] Preferably, the resin adsorption module is a mixture of ion exchange resin having strong adsorption capacity for viruses and bacteria and chitosan or dextran particles.

[0009] Preferably, the resin adsorption module is a mixture of porous ion exchange resin, sialylated ion exchange resin or / and chitosan;

[0010] Preferably, the resin adsorption module is cellulose, dextran and / or agarose gel.

[0011] Preferably, the resin adsorption module is a cylindrical, rectangular, sheet-like or beaded structure arranged in the package.

[0012] Preferably, the negative pressure device is a rubber suction ball, and the rubber suction ball is sleeved on the negative pressure port of the sample storage bottle.

[0013] Preferably, the sample storage bottle is a glass bottle, and a bottle cap is screwed and fixed at the bottle mouth of the glass bottle;

[0014] The sample preservation solution is a physiological buffer solution, which includes serum-free cell culture solution, Hank's buffer solution, PBS phosphate buffer solution, TRIS buffer solution or physiological saline solution; the physiological buffer solution is also added with phenol red indicator and composite broad-spectrum antibiotics, including penicillin, streptomycin and amphotericin B.

[0015] Preferably, it also includes a filter paper strip, which is placed in the package and connected to the resin adsorption module. A wet-printed marking line is printed on the filter paper strip, and the wet-printed marking line is engraved with water-chromogenic ink.

[0016] Compared with the prior art, the advantages of the present invention are that by providing a resin adsorption module in the sampler of the saliva collection device of the present invention, the collection efficiency of pathogens and other biological components to be detected can be improved, that is, the concentration of the extracted substances in the sample can be increased.

[0017] During the sampling process, the wet print mark on the filter paper strip in the sampler can be used to observe whether the sampling is sufficient, thereby ensuring the accuracy of the sampling and improving the sampling efficiency.

[0018] The operation is simple, and ordinary adults can implement it immediately by following the illustrated instructions without professional training;

[0019] The combination of the sampler and the sample storage bottle can facilitate the short-term storage of samples at normal room temperature. It is suitable for remote delivery, convenient sample transportation and guaranteed sampling quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 Schematic diagram of the structure of a saliva collection device in an embodiment of the present invention;

[0021] Figure 2 It is a structural schematic diagram of a sampler in an embodiment of the present invention;

[0022] Figure 3 This is a flow chart of the use of the sampler and the sample storage bottle in an embodiment of the present invention. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be further described below.

[0024] like Figure 1 As shown, the present invention provides a saliva collection device with a disease enrichment adsorption structure, comprising a sampler 1 and a sample storage bottle 2;

[0025] like Figure 2 As shown, the sampler 1 includes a hollow package 4, a resin adsorption module 3 and a negative pressure control device 5; the package 4 is provided with a collection port and a negative pressure port; the resin adsorption module 3 is placed in the package 4 and is arranged at one end of the package 4 close to the collection port; the negative pressure control device 5 is detachably connected to the negative pressure port of the package 4; it also includes a filter paper strip 6, the filter paper strip 6 is placed in the package 4, the filter paper strip 6 is connected to the resin adsorption module 3, and a wet print marking line 7 is printed on the filter paper strip 6, and the wet print marking line 7 is engraved with water-chromogenic ink.

[0026] In this embodiment, the method of using the sampler 1 is as follows: the user operates the negative pressure control device 5 to generate negative pressure inside the package 4, at which time the virus is adsorbed onto the resin adsorption module 3. When the resin adsorption module 3 is close to saturation in adsorbing the virus, moisture will extend along the resin adsorption module 3 to the filter paper strip 6. When the wet-printed mark line 7 on the filter paper strip 6 is moistened and changes color when exposed to water, it indicates that the sampler 1 has collected a sufficient amount of saliva sample as set; wherein, the ink that develops color when exposed to water does not have any damaging effect on the sample.

[0027] In this embodiment, the resin adsorption module 3 is a mixture of ion exchange resin and chitosan or dextran particles solidified with a special virus receptor structure that have strong adsorption capacity for a variety of viruses and bacteria; it is composed of a non-toxic porous ion exchange resin, a sialylated ion exchange resin and / or a chitosan mixture; the resin adsorption module 3 can also be cellulose, dextran and / or agarose gel; it can be selected according to the needs of actual application.

[0028] Given the high affinity of the surface groups of ion exchange resins, sialic acid and chitosan molecules for a variety of viral particles, including influenza viruses and COVID-19 novel coronaviruses, during the sampling process, the resin adsorption module 3 can capture viral particles in saliva and bind them within the module, thereby increasing the concentration of viral particles in the collected sample. The affinity capture activity of the adsorption module is also effective for bacterial and fungal microorganisms, shed endothelial cells, and extracellular particles. Therefore, the resin adsorption module 3 will improve the collection efficiency of potential pathogens or other biological components.

[0029] The resin adsorption module 3 is a cylindrical, rectangular, sheet-like or beaded structure arranged in the package 4. In this embodiment, the resin adsorption module 3 adopts a multi-layer sheet structure 12 to facilitate layered adsorption of viruses.

[0030] In this embodiment, the package 4 is a hollow glass package 4, the package 4 is a columnar structure, the head end of the package 4 is a collection port, and the tail end of the package 4 is a negative pressure port, so as to facilitate observation of the sample collection situation in the package 4.

[0031] In this embodiment, the sample storage bottle 2 is a glass bottle 8, a bottle cap 10 is screwed and fixed at the bottle mouth of the glass bottle, and a sealing cover 11 is provided between the bottle cap 10 and the bottle mouth; the sample storage bottle 2 is preset with sample preservative liquid; after the sampler 1 takes the sample, it is inserted into the sample storage bottle 2.

[0032] In actual application, after the sampler 1 takes a sample, it can be directly placed in the sample storage bottle 2, and the negative pressure control device 5 can be removed for preservation. In this embodiment, the negative pressure control device 5 adopts a rubber suction ball, which is sleeved on the negative pressure port of the sample storage bottle 2. The operator squeezes the rubber suction ball to achieve a negative pressure state inside the sampler 1, thereby achieving adsorption and removal of the virus sample. When the sampler 1 is placed in the sample storage bottle 2, the rubber suction ball is squeezed at the same time to discharge part of the saliva sample into the storage bottle, and then the rubber suction ball is removed from the negative pressure port of the sampler 1, and the bottle cap 10 is screwed on, the saliva sample can be safely stored, and then before the sample is sent to the laboratory for testing, the sample can be stored for a short time to ensure the quality of sample collection. After being sent to the laboratory, the bottle cap 10 is opened, and the sample can be directly sucked from the upper opening of the sampler 1. The operation is simple, convenient, and safe, and the risk of sample contamination or leakage is greatly reduced. For some detection equipment that requires a carrier, the sample storage bottle 2 containing the sample can be directly matched with the detection equipment to complete the detection.

[0033] In this embodiment, the sample preservation solution is a physiological buffer 9, which includes serum-free cell culture medium, Hank's buffer, PBS phosphate buffer, TRIS buffer or physiological saline; phenol red indicator and composite broad-spectrum antibiotics including penicillin, streptomycin and amphotericin B are also added to the physiological buffer 9. For the collection of specific virus samples such as COVID-19 novel coronavirus samples, the buffer may also be added with an RNase inhibitor diethylpyrocarbonate (DEPC) that helps stabilize RNA. The antibiotic helps to inhibit the growth of potential microorganisms during short-term storage, and the phenol red indicator helps indicate whether the pH in the sample buffer changes during storage.

[0034] The above is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any technician in the relevant technical field, without departing from the scope of the technical solution of the present invention, makes any form of equivalent replacement or modification to the technical solution and technical content disclosed in the present invention, which does not depart from the content of the technical solution of the present invention and still falls within the protection scope of the present invention.

Claims

1. A saliva collection device with a disease enrichment adsorption structure, characterized in that: Including sampler and sample storage bottle; The sampler comprises a hollow package, a resin adsorption module and a negative pressure control device; the package is provided with a collection port and a negative pressure port; the resin adsorption module is placed in the package and is arranged at one end of the package close to the collection port; the negative pressure control device is detachably connected to the negative pressure port of the package; The sample storage bottle is preset with a sample preservative solution; after the sampler takes a sample, it is inserted into the sample storage bottle; The sample storage bottle is a glass bottle, and a bottle cap is screwed and fixed at the bottle mouth of the glass bottle; The sample preservation solution is a physiological buffer solution, which includes serum-free cell culture solution, Hank's buffer solution, PBS phosphate buffer solution, TRIS buffer solution or physiological saline solution; the physiological buffer solution is also added with phenol red indicator and composite broad-spectrum antibiotics, including penicillin, streptomycin and amphotericin B; After the sampler takes the sample, it is directly placed in the sample storage bottle, and the negative pressure control device is removed for preservation; the sample is stored for a short time, and after being sent to the laboratory, the bottle cap is opened and the sample is directly sucked from the opening at the upper end of the sampler; It also includes a filter paper strip, which is placed in the package, connected to the resin adsorption module, and printed with a wet-printed marking line, which is engraved with water-chromogenic ink; The package is a hollow glass package, the package is a columnar structure, the head end of the package is the collection port, and the tail end of the package is the negative pressure port; The negative pressure control device is a rubber suction ball, and the rubber suction ball is sleeved on the negative pressure port of the package.

2. The saliva collection device with a disease-enriching adsorption structure according to claim 1, characterized in that: The resin adsorption module is a mixture of ion exchange resin and chitosan or dextran particles having strong adsorption capacity for viruses and bacteria.

3. The saliva collection device with a disease-enriching adsorption structure according to claim 2, characterized in that: The resin adsorption module is a mixture of porous ion exchange resin, sialylated ion exchange resin or / and chitosan.

4. The saliva collection device with a disease-enriching adsorption structure according to claim 1, characterized in that: The resin adsorption module is cellulose, dextran or / and agarose gel.

5. The saliva collection device with a disease-enriching adsorption structure according to claim 1, characterized in that: The resin adsorption module is a cylindrical, rectangular, sheet-shaped or beaded structure arranged in the package.

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