Reagent adding device and method

Abstract

The present invention discloses a reagent adding device and method. The reagent adding device is used for processing slices, comprising: a slide stage, the slide stage defining a accommodating cavity, the accommodating cavity being used to accommodate the slices; a liquid inlet pipe, the liquid inlet pipe being connected to the accommodating cavity, the liquid inlet pipe being used to transport reagents toward the accommodating cavity to submerge the slices; a liquid discharge pipe, the liquid discharge pipe being connected to the accommodating cavity, the liquid discharge pipe being used to discharge the reagents in the accommodating cavity. The reagent adding method comprises the following steps: placing a plurality of slices in the accommodating cavity of the slide stage; transporting reagents toward the accommodating cavity and submerging the slices; and discharging the reagents in the accommodating cavity. When using the device and method of the present invention to process slices, the experimental time can be shortened to a certain extent and the experimental efficiency can be improved.

Description

Technical Field

[0001] The present invention relates to the biological field, and in particular to a reagent adding device and method. Background Art

[0002] Biological experiments are often complex and complex. For example, immunohistochemistry involves multiple steps, including slide loading, dewaxing and rehydration, antigen retrieval, blocking, sealing, addition of primary antibodies, washing, addition of secondary antibodies, color development, counterstaining, clearing, sealing, and unloading. In related technologies, each step involves dripping the corresponding reagent onto each slice using a pipette, which results in long experimental times and low efficiency. Summary of the Invention

[0003] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides a reagent adding device, which can shorten the experimental time and improve the experimental efficiency to a certain extent when processing slices.

[0004] The present invention also provides a reagent adding method, which can shorten the experimental time and improve the experimental efficiency to a certain extent when processing slices.

[0005] In a first aspect, an embodiment of the present invention provides a reagent adding device for processing a slice, comprising:

[0006] A slide stage, wherein the slide stage defines a receiving cavity for receiving the slice;

[0007] a liquid inlet pipe, the liquid inlet pipe being in communication with the accommodating cavity and being used for transporting a reagent toward the accommodating cavity to submerge the slice;

[0008] A drain pipe is connected to the accommodating cavity and is used to discharge the reagent in the accommodating cavity.

[0009] The reagent adding device of the embodiment of the present invention has at least the following beneficial effects: the reagent can flow into the holding cavity of the slide stage through the liquid inlet tube and immerse the slices on the slide stage to participate in the reaction. After the reaction is completed, the reagent can flow out of the holding cavity through the liquid discharge tube. For some universal reagents, using this device can allow the reagent to reach multiple slices in a short time and can be quickly discharged in a short time, which can shorten the experimental time and improve the experimental efficiency to a certain extent.

[0010] According to some other embodiments of the reagent adding device of the present invention, the slide stage includes a carrying platform and a fence, the carrying platform is used to place the slice, the fence surrounds the carrying platform, and the carrying platform and the fence define the accommodating cavity.

[0011] According to some other embodiments of the reagent adding device of the present invention, the liquid inlet pipe and the liquid discharge pipe are respectively located on both sides of the slide stage, and the height of the placement surface on the slide stage for placing the slices gradually decreases in the direction from the outlet of the liquid inlet pipe to the inlet of the liquid discharge pipe.

[0012] According to some other embodiments of the present invention, the reagent adding device further includes an inlet pump and a discharge pump, wherein the inlet pump is connected to the inlet pipe, and the discharge pump is connected to the discharge pipe. The inlet pump is used to draw the reagent into the containing cavity, and the discharge pump is used to draw the reagent out of the containing cavity.

[0013] According to some other embodiments of the present invention, the reagent adding device further includes a sample adding drive and a sample adding member, wherein the sample adding member is connected to the sample adding drive, the sample adding drive is used to drive the sample adding member to move, and the sample adding member is used to drip the reagent onto the slice.

[0014] According to some other embodiments of the present invention, the reagent adding device further includes a first loader, a second loader and a loading drive. The first loader and the second loader are both connected to the loading drive. The first loader can be driven by the loading drive to move toward the slide stage to load the slide, and the second loader can be driven by the loading drive to move toward the slide stage to load the coverslip.

[0015] According to some other embodiments of the reagent adding device of the present invention, the first loading member includes a first suction cup having a hollow portion provided thereon. The first suction cup is used to absorb the glass slide, and the hollow portion is used to avoid the slide area of ​​the glass slide.

[0016] According to some other embodiments of the present invention, the reagent adding device further includes a cover plate and a cover plate driving component, wherein the cover plate is connected to the cover plate driving component, and the cover plate can be moved to the top of the slide stage under the drive of the cover plate driving component, and at least two of the following functional components are provided on the cover plate: a heating component, a glue injection component and a code scanning component.

[0017] According to some other embodiments of the present invention, the reagent adding device further includes a plurality of reagent boxes and liquid guide tubes, each of the liquid guide tubes is connected to one of the reagent boxes, and each of the liquid guide tubes can be connected to the liquid inlet tube.

[0018] In a second aspect, an embodiment of the present invention provides a reagent addition method, comprising the following steps:

[0019] S10 placing a plurality of slices in a receiving cavity of a slide stage;

[0020] S20: transporting a reagent into the containing cavity to submerge the slice;

[0021] S30: Discharge the reagent in the containing cavity.

[0022] The reagent adding method of the embodiment of the present invention has at least the following beneficial effects: the reagent flows into the holding cavity of the slide stage and immerses the slices on the slide stage to participate in the reaction. After the reaction is completed, the reagent flows out of the holding cavity. For some universal reagents, using this method can allow the reagent to reach multiple slices in a short time and can be quickly discharged in a short time, which can shorten the experimental time to a certain extent and improve the experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the overall structure of the reagent adding device in the first embodiment;

[0024] Figure 2 yes Figure 1 Schematic diagram of the internal structure of the reagent adding device;

[0025] Figure 3 yes Figure 1 Schematic diagram of the structure of the middle slide stage and cover plate;

[0026] Figure 4 yes Figure 1 Schematic diagram of the structure of the liquid inlet pipe and the first reagent box and other components;

[0027] Figure 5 is a flow chart of the reagent addition method.

[0028] Reference numerals:

[0029] The slide stage 100, the groove 110, the first pole 120, the liquid inlet tube 200, the outlet 210, the liquid guide tube 230, the liquid discharge tube 300, the first sample adding part 410, the second sample adding part 420, the first loading part 510, the first suction cup 511, the second loading part 520, the second suction cup 521, the slide loading slot 610, the cover glass storage slot 620, the slide unloading slot 630, the cover plate 700, the glue injection part 710, the code scanning part 720, the second pole 730, the first reagent box 810, the second reagent box 820, the waste liquid box 830, the first cleaning tank 910, the second cleaning tank 920, and the outer shell 1000. DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the concept and technical effects of the present invention in conjunction with the embodiments to fully understand the purpose, features and effects of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention.

[0031] In the description of the embodiments of the present invention, if orientation descriptions are involved, the orientations or positional relationships indicated by "up", "down", "front", "back", "left", "right", etc. are based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limiting the present invention.

[0032] In the description of the embodiments of the present invention, if a certain feature is referred to as being "set", "fixed", "connected" or "installed" on another feature, it may be directly set, fixed or connected on the other feature, or it may be indirectly set, fixed, connected or installed on the other feature. In the description of the embodiments of the present invention, if "several" is involved, it means more than one; if "multiple" is involved, it means more than two; if "greater than", "less than" or "exceeds" is involved, it should be understood as not including the number itself; if "above", "below" or "within" is involved, it should be understood as including the number itself. If "first" or "second" is involved, it should be understood as being used to distinguish technical features, and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0033] Reference Figure 1 and Figure 2 The reagent adding device in this embodiment includes components such as a slide stage 100, a liquid inlet pipe 200 and a liquid discharge pipe 300. A heating wire is also provided inside the slide stage 100 for use when baking slices. The slide stage 100 defines a accommodating cavity in which slices can be placed. The first reagent box 810 is located below the slide stage 100 and is filled with reagents. The inlet of the liquid inlet pipe 200 is connected to the first reagent box 810, and the outlet is connected to the accommodating cavity. The reagents in the first reagent box 810 can flow into the accommodating cavity through the liquid inlet pipe 200 and submerge the slices to participate in the reaction. The waste liquid tank 830 is located on one side of the slide stage 100 and is used to collect waste liquid after the reaction. The inlet of the liquid discharge pipe 300 is connected to the accommodating cavity, and the outlet is connected to the waste liquid tank 830. The reagents remaining after the reaction can flow out of the accommodating cavity through the liquid discharge pipe 300 and flow into the waste liquid tank 830. In addition, a plurality of grooves 110 are provided on the slide stage 100, the size of which matches the glass slide, and the glass slide is fixed in the groove 110. However, it should be noted that after the glass slide is inserted into the groove 110, its top surface cannot be lower than the top surface of the groove 110, so as to avoid the residual liquid after the reaction cannot be drained away. A shell 1000 is provided on the outside of each of the above components to protect the above components. An exhaust gas sensor can also be provided on the inner wall of the shell 1000 to detect the amount of exhaust gas in the shell 1000 so as to adjust the exhaust fan air volume in time.

[0034] In this embodiment, the reagent reaches the slide stage 100 through the liquid inlet tube 200 and submerges the slide, allowing the reagent to reach each section as quickly as possible. After the reaction is completed, the reagent flows into the waste liquid tank 830 through the liquid discharge tube, and the liquid discharge speed is also relatively fast. When the same reagent needs to be added to all sections, the device of this embodiment can shorten the reaction time and improve the reaction efficiency compared to the conventional method of adding the reagent to each slide sequentially with a sample pen.

[0035] In some embodiments, the slide stage 100 includes a carrying platform and a fence, the slices are placed on the carrying platform, and the fence is arranged around the carrying platform. The carrying platform and the fence define the above-mentioned accommodating cavity. Two openings can be set on the fence, which are respectively connected to the outlet 210 of the liquid inlet pipe 200 and the inlet of the liquid discharge pipe 300. Alternatively, the outlet of the liquid inlet pipe 200 can be set above the fence. Setting the fence can block the reagent, ensuring that it can only reach the slide stage 100 from the liquid inlet pipe 200 and submerge the slices. After the reaction is completed, it can only flow out of the slide stage 100 from the liquid discharge pipe 300. A valve can also be set at the inlet of the liquid discharge pipe 300. When the reaction is completed, the valve can be opened to prevent some reagents from being discharged before the reaction is completed.

[0036] Alternatively, in some embodiments, a fence may not be provided, and a larger recess may be directly provided on the top surface of the slide stage 100. The slice is placed in the recess, which forms the aforementioned receiving cavity. Two openings may be provided on the slide stage 100 that communicate with the recess, and these two openings may be connected to the outlet 210 of the liquid inlet pipe 200 and the inlet of the liquid discharge pipe 300, respectively. Alternatively, the outlet of the liquid inlet pipe 200 may be located above the recess.

[0037] In some embodiments, an inlet pump and a drain pump (omitted in the figure) are further provided. The inlet pump is connected to the inlet pipe 200, and the drain pump is connected to the drain pipe 300. The inlet pump draws the reagents from the first reagent tank 810 into the inlet pipe 200 and into the holding chamber. The drain pump draws the reacted reagents from the holding chamber into the drain pipe 300 and into the waste liquid tank 830. Providing the inlet pump and drain pump can facilitate the inflow and outflow of reagents.

[0038] Reference Figure 2 and Figure 3In some embodiments, the inlet pipe 200 and the drain pipe 300 are located on either side of the slide stage 100, and the height of the placement surface of the slide stage 100 (i.e., the top surface of the slide stage 100) for placing the slides (i.e., the top surface of the slide stage 100) gradually decreases from the outlet of the inlet pipe 200 to the inlet of the drain pipe 300. This allows the reagents to flow toward the inlet of the drain pipe 300 under the action of gravity when they enter the slide stage 100, allowing the reagents to cover the slides more quickly. After the reaction is completed, the valve of the drain pipe 300 is opened and the drain pump is activated, allowing the reagents to flow more easily into the drain pipe 300, facilitating the rapid discharge of any residual reagents. Specifically, the top surface of the slide stage 100 can be rectangular, and the outlet of the inlet pipe 200 and the inlet of the drain pipe 300 can be located at opposite ends of the diagonal of the rectangle. Along this diagonal, the height of the slide stage 100 gradually decreases from the outlet of the inlet pipe 200 to the inlet of the drain pipe 300.

[0039] It should be noted that in the above embodiment, since the top surface of the slide stage 100 has a certain inclination, when adding reagents, it is necessary to ensure that after the reagents cover the slide stage 100, they can at least submerge the slice at the highest position, so as to avoid insufficient reagent on the slice near the outlet of the liquid inlet tube 200, which makes it impossible to react normally.

[0040] In some embodiments, the top surface of the slide stage 100 is located within the height range of the inlet of the drainage tube 300, that is, the bottom surface of the inlet of the drainage tube 300 is located below the top surface of the slide stage 100, and the top surface of the inlet of the drainage tube 300 is located above the top surface of the slide stage 100. In this way, after the drainage pump is turned on, the reagent can flow into the drainage tube 300 more smoothly.

[0041] In some embodiments, a sample loading unit (e.g., a first sample loading unit 410 and a second sample loading unit 420) and a sample loading drive (omitted in the figure) are further provided. The sample loading unit is connected to the sample loading drive, which drives the sample loading unit to complete sample loading. A conventional sample loading pen can be used for the sample loading unit, while a three-axis motion module or a robotic arm can be used for the sample loading drive. A second reagent tank 820 is also provided on one side of the slide stage 100. The sample loading drive drives the sample loading unit to the second reagent tank 820 to draw the required reagent, and then to the target slice for dropwise addition. If different reagents are required for each slice, the sample loading unit can be used to add reagents sequentially. For more expensive reagents, the sample loading unit can also be used to add reagents to reduce costs and waste. In this embodiment, if the reagents required for all slices are the same or are not expensive, the reagents can be added through the liquid inlet pipe 200 and the liquid outlet pipe 300. If the reagents required for each slice are different or are expensive, the sample loading unit can be used for addition. The combination of these two loading methods provides greater flexibility.

[0042] A cleaning tank (e.g., first cleaning tank 910 and second cleaning tank 920) is provided on one side of the slide stage 100. The cleaning tank is filled with cleaning fluid for cleaning the sample loading device. If the sample loading device requires different reagents each time, it must be cleaned before the next addition to prevent contamination of the reagents.

[0043] In some embodiments, a slide loading slot 610, a cover glass storage slot 620, and a slide unloading slot 630 are further provided on one side of the slide stage 100. The slide loading slot 610 contains a slide, the cover glass storage slot 620 contains a cover glass, and the slide unloading slot 630 contains the sealed slide and cover glass combination. Furthermore, a first loading member 510, a second loading member 520, and a loading driver (omitted in the figure) are provided. The loading driver can be a three-axis motion module or a robotic arm. The loading driver drives the first loading member 510 to remove a slide from the slide loading slot 610 and transfer it to the groove 110 on the slide stage 100. After the reaction is completed, the loading driver drives the second loading member 520 to remove a cover glass from the cover glass storage slot 620 and transfer it to the slide to complete the sealing. After the sealing is completed, the loading driver drives the second loading member 520 to place the finished product in the groove 110 into the slide unloading slot 630. Because sections are placed on the slide, to prevent contamination of the slide, it is necessary to avoid contact with the slide area (the area for placing sections) when removing the slide. However, a cover glass does not require a specific contact position. Therefore, using matching loading members when transporting slides and cover glasses can achieve better matching.

[0044] In some embodiments, the first loader 510 includes a first suction cup 511, and the second loader 520 includes a second suction cup 521. The slide and cover glass are taken out by suction, which can achieve gentle handling and reduce the chance of damaging the slide and cover glass. The first suction cup 511 is provided with a hollow portion to avoid the slide area of ​​the slide, and the second suction cup 521 is not restricted. Specifically, the first suction cup 511 can be set to a ring shape, the hollow part in the middle of the ring is the hollow part, and the ring part is the position for sucking and fixing the slide. Alternatively, it can be set to other shapes, such as digging out an ellipse, polygon or other irregular shape on the suction cup, which can be selected according to the shape of the slice.

[0045] In some embodiments, a cover plate 700 and a cover plate driver (omitted in the figure) are also provided. The cover plate driver can be a three-axis motion module or a robotic arm. The cover plate 700 is equipped with at least two of the following functional components: a heater, a glue injection component, and a barcode scanner. For example, the cover plate 700 is equipped with a heater and a glue injection component 710 (omitted in the figure). The heater can be formed by heating wires or other means at the bottom of the cover plate 700. The cover plate driver drives the cover plate 700 to move above the slide stage 100 to heat the reagents. The distance between the cover plate 700 and the slide stage 100 ranges from 0.1 mm to 0.3 mm. As the cover plate 700 moves toward the slide stage 100, the glue injection component 710 passes over each row of slides in sequence and injects glue onto the slides. After injection, a cover glass is placed on the slides to seal the slides. In this embodiment, the cover plate 700 not only performs heating but also glue injection, integrating the components for a more compact structure and reduced space requirements.

[0046] Furthermore, the cover plate 700 is provided with a heating element, a glue injection element 710 and a code scanning element 720. When the cover plate 700 passes through each row of glass slides in turn, the code scanning element 720 completes the scanning of the QR code on each glass slide to facilitate subsequent tracking. This embodiment further integrates the components to make the structure more compact and occupy less space.

[0047] In some embodiments, a power supply is also included. A first pole 120 is provided on the slide stage 100, and a second pole 730 is provided on the cover plate 700. The first pole 120 and the second pole 730 can be made of platinum wire, and the two are electrically connected to the two poles of the power supply respectively. After the cover plate 700 is moved above the slide stage 100, the power is turned on, and a working electric field is formed between the slide stage 100 and the cover plate 700. The working electric field needs to be a non-uniform electric field, and the field strength gradually increases from the cover plate 700 to the slide stage 100. In a non-uniform electric field, the reagent will produce a dielectrophoresis effect and move in the direction of the strong field strength, thereby accelerating the reaction with the slice and shortening the reaction time. For example, in an immunohistochemistry experiment, after adding antibodies, the binding of antibodies to antigens can be accelerated.

[0048] Reference Figure 1 and Figure 3 In some embodiments, multiple first reagent tanks 810 and liquid conduits 230 are provided. Each first reagent tank 810 contains a different reagent and is connected to the liquid inlet pipe 200 via a liquid conduit 230. Each liquid conduit 230 is provided with a valve. To add a reagent, the valve of the liquid conduit 230 connected to the first reagent tank 810 containing the reagent is opened, the valves of the other liquid conduits 230 are closed, and the liquid inlet pump is turned on to add the desired reagent. One of the first reagent tanks 810 contains a cleaning solution. Before adding a different reagent, the cleaning solution must be added to clean the solution to avoid affecting the accuracy of subsequent reactions.

[0049] Reference Figure 5 In some embodiments, a method for adding reagents to slices is to first place multiple slices in a holding cavity of a slide stage, deliver reagents into the holding cavity, submerge the slices in the holding cavity, and react. After the reaction is complete, the reagents in the holding cavity are discharged. This reagent addition method can be implemented using the reagent addition device described in the above embodiments.

[0050] In conjunction with the above embodiments, when using the device of the present invention to perform immunohistochemistry, steps such as slide loading, baking, dewaxing and rehydration, antigen retrieval, blocking, blocking, adding primary antibodies, washing, adding secondary antibodies, color development, counterstaining, clearing, sealing, and slide unloading can be performed. During operation, the slide with the section attached is placed on the groove 110 using the first loading component 510. If baking is required, this can be achieved using the heating wire inside the slide stage 100. After baking, the dewaxing solution, ethanol (for rehydration), antigen retrieval solution, blocking agent, and blocking solution are sequentially delivered to the slide stage 100 through the liquid inlet pipe 200 and the liquid outlet pipe 300. Between each step, a cleaning solution is required. Then, the cover plate 700 is moved to the top of the slide stage 100 to heat the blocking solution. After heating is completed, while the cover plate 700 is repositioned, the code scanning component 720 scans the QR code on each slide. Then, the primary antibody is added dropwise through the first sample adding member 410. After the addition is completed, the first sample adding member 410 is cleaned in the first cleaning tank 910. At the same time, the cover plate 700 is moved again to the top of the slide stage 100 to heat the primary antibody. After heating, a cleaning solution is delivered to the slide stage 100 through the liquid inlet pipe 200 for cleaning. After cleaning is completed, the cleaning solution is discharged from the liquid discharge pipe 300. Next, the secondary antibody and color developer are added dropwise through the first sample adding member 410, and the process is similar to the addition of the primary antibody. After the color development is completed, the counterstain solution is added dropwise through the second sample adding member 420. After the counterstaining is completed, the second sample adding member 420 is cleaned in the second cleaning tank 920. A separate sample adding member is set up for counterstaining because the counterstain solution is not easy to completely clean. If the sample adding member is shared with other reagents, it may affect the performance of other reagents. Providing a separate cleaning tank for the second sample adding member 420 is to prevent contamination of the first sample adding member 410 by the counterstain solution during cleaning. After redyeing is complete, a transparent liquid is delivered to the slide stage 100 via the liquid inlet tube 200. After the transparent liquid has been transparent for a certain period of time, the cover plate 700 is moved again to the top of the slide stage 100. Simultaneously, the adhesive dispensing unit 710 drips the mounting medium onto the slide. The second loading unit 520 then places the cover glass on top of the slide to complete the mounting process. After the mounting medium has dried, the mounted sections can be removed.

[0051] While the embodiments of the present invention have been described in detail above with reference to the accompanying drawings, the present invention is not limited to the embodiments described above. Various modifications may be made within the scope of knowledge possessed by a person skilled in the art without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof may be combined with one another unless there is a conflict.

Claims

1. A reagent adding device for processing slices, characterized in that: include: A slide stage, wherein the slide stage defines a receiving cavity for receiving the slice; a liquid inlet pipe, the liquid inlet pipe being in communication with the accommodating cavity and being used for transporting a reagent toward the accommodating cavity to submerge the slice; a drain pipe, the drain pipe being in communication with the accommodating cavity and being used to discharge the reagent in the accommodating cavity; wherein the liquid inlet pipe and the liquid discharge pipe are respectively located on both sides of the slide stage, and the height of the placement surface on the slide stage for placing the slice gradually decreases in the direction from the outlet of the liquid inlet pipe to the inlet of the liquid discharge pipe, the reagent adding device also includes a sample adding drive and a sample adding member, the sample adding member is connected to the sample adding drive, the sample adding drive is used to drive the sample adding member to move, and the sample adding member is used to drip the reagent onto the slice, the reagent adding device also includes a power supply and a cover plate, a first pole is provided on the slide stage, and a second pole is provided on the cover plate, the first pole and the second pole are respectively electrically connected to the two poles of the power supply, after the cover plate moves above the slide stage, the power supply can form a working electric field between the slide stage and the cover plate, the working electric field is a non-uniform electric field, and the field strength from the cover plate to the slide stage gradually increases, so that the working electric field can drive the reagent to move in the direction from the cover plate to the slide stage; The reagent adding device also includes a cover plate driving component, the cover plate is connected to the cover plate driving component, and the cover plate can be moved to the top of the slide table under the drive of the cover plate driving component. The cover plate is also provided with a heating component, a glue injection component and a code scanning component.

2. The reagent adding device according to claim 1, characterized in that The slide stage includes a carrying platform and a fence. The carrying platform is used to place the slices. The fence surrounds the carrying platform. The carrying platform and the fence define the accommodating cavity.

3. The reagent adding device according to claim 1, characterized in that It also includes an inlet pump and a discharge pump, the inlet pump is connected to the inlet pipe, the discharge pump is connected to the discharge pipe, the inlet pump is used to draw the reagent into the containing cavity, and the discharge pump is used to draw the reagent out of the containing cavity.

4. The reagent adding device according to claim 1, characterized in that It also includes a first loading member, a second loading member and a loading drive member. The first loading member and the second loading member are both connected to the loading drive member. The first loading member can move toward the slide stage to load the slide under the drive of the loading drive member. The second loading member can move toward the slide stage to load the cover glass under the drive of the loading drive member.

5. The reagent adding device according to claim 4, characterized in that The first loading member includes a first suction cup, which is provided with a hollow portion. The first suction cup is used to absorb the glass slide, and the hollow portion is used to avoid the loading area of ​​the glass slide.

6. The reagent adding device according to claim 1, characterized in that It also includes a plurality of reagent boxes and liquid guide tubes, each of the liquid guide tubes is connected to one of the reagent boxes, and each of the liquid guide tubes can be connected to the liquid inlet tube.

7. A reagent adding method, characterized in that: The method using the reagent adding device according to any one of claims 1 to 6 comprises the following steps: S10, placing multiple slices in the accommodating cavity of the slide stage; S20, transporting a reagent into the containing cavity to submerge the slice; S30, discharging the reagent in the containing cavity; A heating element is provided at the bottom of the cover plate. When the cover plate moves to the top of the slide stage, the heating element heats the reagent. After the cover plate moves to the top of the slide stage, the power is turned on to form a working electric field between the slide stage and the cover plate. When the cover plate passes over the slide, the code scanning element scans the QR code on the slide. When the cover plate moves toward the slide stage, the glue injection element passes over the slide and injects glue onto the slide.

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