A multi-band light source gating device and microscopic imaging system

Abstract

The present invention provides a multi-band light source gating device and a microscopic imaging system, wherein the gating device includes a fixed component and a moving part. The fixed component is provided with a power-on position; the moving part is movably provided on the fixed component, and at least three installation cavities are provided on the moving part, wherein a wide-spectrum light source and at least two narrow-spectrum light sources of different wavelengths are respectively installed in the three installation cavities. The moving part is moved to move the required light source to the power-on position so that the positive and negative poles of the light source are respectively connected to the power supply and energized. The multi-band light source gating device is applied to the microscopic imaging system. During ordinary imaging, the wide-spectrum light source is moved to the power-on position so that the light source is coaxial with the illumination optical path, thereby meeting the ordinary imaging illumination requirements; during fluorescence imaging, the narrow-spectrum light source of the required band is moved to the power-on position according to the requirements of the sample to be observed so that the light source is coaxial with the illumination optical path, thereby meeting the fluorescence imaging illumination requirements and ensuring the imaging effect of the microscopic imaging system.

Description

Technical Field

[0001] The invention relates to the technical field of microscopic imaging illumination, and in particular to a multi-band light source gating device and a microscopic imaging system. Background Art

[0002] The light source device is one of the key components of the microscopic imaging system. For microscopic imaging systems, halogen lamps and LED lamps are usually used. The advantage of halogen lamps is that the spectrum is wide and close to white light, but its disadvantages are high power and high heat generation, and it is generally not used for fluorescence excitation. The advantages of LED lamps are high luminous efficiency, narrow monochromatic light spectrum (width 20-30nm), long life, and small size, which can be used as fluorescence excitation light.

[0003] Fluorescence microscopy systems usually take into account both ordinary imaging and fluorescence imaging. Ordinary imaging requires a wide-spectrum light source, while fluorescence imaging uses narrow-spectrum light sources of different bands according to the needs of the sample to be observed. Conventional light source devices cannot achieve the gating of wide-spectrum light sources and narrow-spectrum light sources of different bands, resulting in a mismatch between the actual light source used and the light source required for the observed sample, and the best microscopy imaging effect cannot be achieved. Summary of the invention

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defect that the light source device in the prior art cannot realize the selection of a wide-spectrum light source and a narrow-spectrum light source of different bands, resulting in a mismatch between the light source actually used and the light source required for observing the sample, and failing to achieve the best microscopic imaging effect.

[0005] To this end, the present invention provides a multi-band light source gating device, comprising

[0006] A fixed assembly having an energized station thereon;

[0007] A movable part is movably arranged on the fixed component; at least three installation cavities are arranged on the movable part, and a wide spectrum light source and at least two narrow spectrum light sources with different wavelengths are respectively installed in the installation cavities. After a certain light source is moved to the power-on station, the positive and negative poles of the light source are respectively connected to the power supply for powering on.

[0008] Optionally, in the above-mentioned multi-band light source gating device, the movable part is a first turntable, the first turntable is rotatably provided on the fixed component, the plurality of mounting cavities are distributed on the same circumference, and the first turntable is rotated to move the light source of the required spectrum to the power-on station for power-on.

[0009] Optionally, the multi-band light source gating device further comprises a conductive slip ring, the fixing assembly comprises a second base, and the first turntable is rotatably disposed on the second base;

[0010] The conductive slip ring is arranged on the second base, and a first conductive component electrically connected to the light source is arranged at the bottom of each light source;

[0011] The multi-band light source gating device also includes a second conductive component disposed on the power-on station, the second conductive component is electrically connected to the conductive slip ring, and when the required light source is rotated to the power-on station, the first conductive component at the bottom of the light source contacts and conducts electricity with the second conductive component.

[0012] Optionally, in the above-mentioned multi-band light source gating device, the first conductive component is ring-shaped, the second conductive component is a conductive spring sheet, and when the first conductive component rotates to the power-on position, its outer wall surface abuts against the conductive spring sheet.

[0013] Optionally, the multi-band light source gating device further comprises a rotating member, which is rotatably disposed at one end of the conductive spring sheet close to the power-on position, and at the power-on position, the first conductive component abuts against the rotating member.

[0014] Optionally, the multi-band light source gating device further includes a rotating shaft, which is arranged at the rotation center of the first turntable and extends toward the second base, and the rotor of the conductive slip ring is fixedly connected to the rotating shaft.

[0015] Optionally, in the above-mentioned multi-band light source gating device, the fixing assembly also includes an upper cover, which is arranged on the second base, and the middle part of the upper cover has a circular opening, and the bottom of the first turntable can be rotatably engaged in the circular opening; the outer periphery of the first turntable has a first flange, and the bottom of the first flange abuts against the upper cover.

[0016] Optionally, the above-mentioned multi-band light source gating device further includes a positioning component, which is telescopically arranged on the second base; after the first turntable rotates into place, the positioning component abuts against the first turntable to fix the first turntable.

[0017] Optionally, in the above-mentioned multi-band light source gating device, the positioning component is a spring plunger.

[0018] The present invention provides a microscopic imaging system, comprising any one of the multi-band light source gating devices described above.

[0019] The technical solution of the present invention has the following advantages:

[0020] 1. In the multi-band light source gating device provided by the present invention, a wide-spectrum light source and at least two narrow-spectrum light sources with different wavelengths are respectively installed in the installation cavity of a movable part. The movable part is moved to move the required light source to the power-on position so that the positive and negative poles of the light source are respectively connected to the power supply and energized. The multi-band light source gating device is applied to a microscopic imaging system. During ordinary imaging, the wide-spectrum light source is moved to the power-on position so that the light source is coaxial with the illumination optical path, thereby meeting the ordinary imaging illumination requirements. During fluorescence imaging, the narrow-spectrum light source of the required band is moved to the power-on position according to the requirements of the sample to be observed so that the light source is coaxial with the illumination optical path, thereby ensuring that the light source used matches the light source of the band required for the observed sample, thereby meeting the fluorescence imaging illumination requirements and ensuring the imaging effect of the microscopic imaging system.

[0021] 2. The microscopic imaging system provided by the present invention includes a multi-band light source selection device, a first base and an illumination module. The multi-band light source selection device can select a narrow spectrum light source or a wide spectrum light source of a suitable band to meet the fluorescence imaging illumination requirements or the general imaging illumination requirements of the microscopic imaging system, thereby ensuring the imaging effect of the microscopic imaging system. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 It is a schematic diagram of the multi-band light source gating device provided in Example 1 of the present invention;

[0024] Figure 2 A cross-sectional view of the multi-band light source gating device provided in Example 1 of the present invention;

[0025] Figure 3 is a schematic diagram of the cooperation between the second conductive component and the first conductive component;

[0026] Figure 4 This is a schematic diagram of the internal structure of the electric control box;

[0027] Figure 5 It is a wiring diagram of the multi-band light source gating device;

[0028] Figure 6 It is a schematic diagram of a multi-band light source gating device;

[0029] Figure 7 is a schematic diagram of a microscopic imaging system provided in Example 2 of the present invention;

[0030] Figure 8 for Figure 7 Schematic diagram after removing the upper cover of the first base.

[0031] Description of reference numerals:

[0032] 101-LED lamp; 102-halogen lamp; 103-first turntable; 1031-first flange; 1041-stator; 1042-rotor; 105-rotating shaft; 106-first conductive component; 107-second conductive component; 1071-fixed shaft; 108-rotating member; 109-second base; 1091-first top plate; 10911-first boss; 1092-first side plate; 110-positioning component; 111-upper cover; 112-bottom cover; 113-first pressure ring; 114-second pressure ring; 115-light homogenizing plate; 116-filter holder; 117-narrowband filter; 118-cable connector; 2-first base; 3-lighting module;

[0033] e01-electrical control box; e11-DC connector; e12-light intensity controller; e13-display; e14-220V power connector; e15-DC switching power supply; e16-push button switch. DETAILED DESCRIPTION

[0034] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0036] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0037] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0038] Example 1

[0039] This embodiment provides a multi-band light source gating device, such as Figure 1 and Figure 2 As shown, it includes a fixed component and a moving part.

[0040] Among them, a power-on station is provided on the fixed component; the movable part is movably arranged on the fixed component, and at least three installation cavities are provided on the movable part. A wide-spectrum light source and at least two narrow-spectrum light sources with different wavelengths are respectively installed in the three installation cavities. After a light source moves to the power-on station, the positive and negative poles of the light source are respectively connected to the power supply for powering on.

[0041] The moving part is moved to move the required light source to the power-on position so that the positive and negative poles of the light source are connected to the power supply and energized respectively. The multi-band light source gating device is applied to the microscopic imaging system. During ordinary imaging, the wide-spectrum light source is moved to the power-on position so that the light source is coaxial with the illumination light path, thereby meeting the ordinary imaging illumination requirements. During fluorescence imaging, the narrow-spectrum light source of the required band is moved to the power-on position according to the requirements of the sample to be observed so that the light source is coaxial with the illumination light path, thereby ensuring that the light source used matches the light source of the band required for the observed sample, thereby meeting the fluorescence imaging illumination requirements and ensuring the imaging effect of the microscopic imaging system.

[0042] Specifically, see Figure 1 , the moving part is a first turntable, the wide spectrum light source is a halogen lamp 102, and the halogen lamp 102 is used to broaden the spectrum of the light source. The narrow spectrum light source includes five LED lamps 101 of different wavelength bands. The fixed component includes a second base 109, an upper cover 111 and a bottom cover 112. The multi-band light source gating device also includes a conductive slip ring, a rotating shaft 105, a first conductive component 106, a second conductive component 107, a rotating part 108 and a positioning component 110.

[0043] See also Figure 1 and Figure 2 The upper cover 111 includes a vertical portion and a horizontal portion. The vertical portion of the upper cover 111 is fixed to the second base 109 by screws. The horizontal portion is arranged at the top of the vertical portion and extends toward the center of the upper cover 111. A circular opening is arranged in the middle of the horizontal portion. The bottom of the first turntable 103 is rotatably clamped in the circular opening of the upper cover 111. The first flange 1031 on the outer periphery of the first turntable 103 is clamped on the top of the horizontal portion to support the first turntable, thereby ensuring smooth rotation of the turntable.

[0044] The first turntable 103 is provided with six installation cavities, the centers of which are distributed on the same circumference. The LED lamp 101 and the halogen lamp 102 are installed in the installation cavities. A light homogenizing plate 115 is provided on the top of the LED lamp 101 and the halogen lamp 102 to improve the uniformity of the light emitted by the light source. A first pressing ring 113 is provided on the top of the light homogenizing plate 115, and the first pressing ring 113 fixes the light homogenizing plate 115 and the light source. A filter seat 116 is provided on the first pressing ring 113 on the top of the five LED lamps 101, and a narrow-band filter 117 is threadedly connected in the filter seat 116 to accurately select the light source of the required wavelength band. A second pressing ring 114 is provided on the top of the narrow-band filter 117.

[0045] The second base 109 includes a first top plate 1091 and a first side plate 1092. A bottom cover 112 is clamped on the bottom inner wall of the first side plate 1092. The bottom cover 112 is spaced apart from the top plate. The middle of the first top plate 1091 extends toward the bottom cover 112 to form a first boss 10911. The first top plate 1091 and the first boss 10911 are provided with a first through hole. The stator 1041 of the conductive slip ring is fixed in the first through hole. The shaft 105 is fixed at the center of the first rotating disk 103 by screws. The shaft 105 is located at the bottom of the first rotating disk 103 and extends toward the bottom cover 112. The shaft 105 is passed through and fixed in the conductive slip ring rotor 1042.

[0046] A first conductive component 106 is provided at the bottom of each LED lamp 101 and halogen lamp 102. The first conductive component 106 is electrically connected to the LED lamp 101 and halogen lamp 102 on the top thereof. Preferably, the first conductive component 106 is a conductive ring with a circular cross section, and the conductive ring is embedded in the mounting cavity of the first rotating disk 103. Figure 3 The second conductive component is arranged on the power-on station. The second conductive component 107 is a conductive spring. The first end of the conductive spring is fixed on the inner wall surface of the vertical part of the upper cover 111, and the second end extends toward the conductive ring. A fixed shaft 1071 is arranged on the wall surface of the second end of the conductive spring facing the rotating shaft 105. The rotating member 108 is rotatably sleeved on the fixed shaft 1071. The second end of the conductive spring is provided with an escape zone for the rotating member 108 to rotate. The rotating member 108 is made of conductive material.

[0047] See also Figure 5 The positive poles of the LED lamp 101 and the halogen lamp 102 are connected to the positive pole of the conductive slip ring rotor 1042 through the positive pole cable, the negative poles of the LED lamp 101 and the halogen lamp 102 are welded to the conductive ring below them, the negative pole of the conductive slip ring rotor 1042 is connected to the conductive spring through the negative pole cable, and the positive pole and negative pole of the conductive slip ring stator 1041 are respectively connected to the positive pole and negative pole of the cable connector 118. The cable connector 118 is connected to an external power supply to power the multi-band light source gating device.

[0048] Specifically, see Figure 4 and Figure 6 The multi-band light source gating device also includes an electric control box e01, which is provided with a DC connector e11, a light intensity controller e12, a display e13, a 220V power connector e14, a DC switch power supply e15, and a button switch e16. External 220V AC power is connected to the inside of the electric control box e01 through the 220V power connector e14, and the other end of the 220V power connector e14 is connected to the 220V AC power connection terminal of the DC switch power supply e15 through a wire. After conversion by the DC switch power supply e15, the 220V AC power is converted into DC power. The DC connection terminal on the DC switch power supply e15 is connected in series with the DC connector e11, the light intensity controller e12, the display e13, and the button switch e16 on the electric control box e01 through a wire, and the DC connector e11 is then connected in series to the cable connector 118 through a cable. When the button switch e16 is pressed, the entire circuit is turned on, and the power supply of the light source to the electric control box e01 is completed. The current of the entire circuit is adjusted by turning the adjustment knobs on the light intensity controller e12 and the display e13. Meanwhile, the display e13 thereon will display the current magnitude, thereby achieving quantitative control of the light intensity of the light source.

[0049] The multi-band light source gating device is applied to the microscopic imaging system, the conductive spring is located on one side of the illumination light path, the LED lamp 101 or the halogen lamp 102 of the required band is rotated to the illumination light path through the first rotating disk 103, the outer wall surface of the conductive ring at the bottom of the light source on the illumination light path abuts against the rotating member 108 and is connected to the conductive spring, and the negative electrode of the light source is connected and lit. The setting of the rotating member 108 makes the conductive ring and the rotating member 108 in close and reliable contact, and at the same time can reduce the friction between the conductive ring and the conductive spring, and increase the service life of the conductive ring and the conductive spring.

[0050] Optimally, the positioning component 110 is a spring plunger. There are two spring plungers, which are symmetrically arranged on the first top plate 1091. When the first turntable 103 rotates, the top of the spring plunger is pressed down. After the first turntable 103 rotates into place, the top of the spring plunger is lifted up and reset by the spring. An inverted "V" groove is provided at the bottom of the first turntable. After the top of the spring plunger is reset, it is snapped into the "V" groove to position the first turntable 103, preventing the first turntable 103 from shaking, and ensuring that the required light source is always aligned with the illumination light path.

[0051] As a first alternative implementation of Example 1, the upper cover may not be provided, and the top of the first top plate may extend upward and toward the center of the second base to form a circular opening for the first turntable to be clamped.

[0052] As a second alternative implementation of Example 1, the rotating member and the fixed shaft may not be provided, and the conductive ring directly contacts the conductive spring after the light source moves to the power-on position. The first conductive component may also be in any other shape, and the second conductive component may also be in a cylindrical, cube, or other shape, as long as the first conductive component moves to the power-on position and can contact the second conductive component to achieve circuit connectivity of the light source.

[0053] As a third alternative implementation of Example 1, the conductive slip ring may not be provided, and the positive pole of the light source may be directly connected to the positive pole of the cable connector, the negative pole of the light source may be connected to the first conductive component, and the second conductive component may be connected to the negative pole of the cable connector. The first conductive component may be moved to the power-on position to contact the second conductive component to achieve circuit connectivity of the light source.

[0054] As a fourth alternative implementation of Example 1, the moving part may also be in other shapes, and the moving part may be slidably disposed on the fixed component along a straight line, and the required light source may be switched by translation.

[0055] Example 2

[0056] This embodiment provides a microscopic imaging system, such as Figure 7 and Figure 8 As shown, it includes the multi-band light source gating device in Example 1, a first base 2 and a lighting module 3.

[0057] The multi-band light source gating device is arranged in the first base. The light source in the power-on position is coaxial with the lighting module. The first flange 1031 partially extends out of the first base 2, making it convenient to rotate the first turntable 103 from outside the first base 2.

[0058] The microscopic imaging system of this structure can select a narrow-spectrum light source or a wide-spectrum light source of a suitable band through a multi-band light source selection device to meet the fluorescence imaging lighting requirements or general imaging lighting requirements of the microscopic imaging system and ensure the imaging effect of the microscopic imaging system.

[0059] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. A multi-band light source gating device, characterized in that: include A fixed assembly having an energized station thereon; A movable member is movably arranged on the fixed component; the movable member is provided with at least three installation cavities, in which a broad spectrum light source and at least two narrow spectrum light sources with different wavelengths are respectively installed, and after a certain light source is moved to the power-on position, the positive and negative electrodes of the light source are respectively connected to the power source for powering on; The moving part is a first rotating disk (103), the first rotating disk (103) is rotatably arranged on the fixed component, the plurality of mounting cavities are distributed on the same circumference, and the first rotating disk (103) is rotated to move the light source of the required spectrum to the power-on position for power-on; the fixed component also includes a conductive slip ring, the fixed component includes a second base (109), the first rotating disk (103) is rotatably arranged on the second base (109); The conductive slip ring is arranged on the second base (109), and a first conductive component (106) electrically connected to the light source is arranged at the bottom of each light source; The multi-band light source gating device further comprises a second conductive component (107) disposed on the power-on position, the second conductive component (107) being electrically connected to the conductive slip ring, and the first conductive component (106) at the bottom of the light source being in contact with the second conductive component (107) for electrical conduction when the required light source is rotated to the power-on position; the first conductive component (106) is ring-shaped, and the second conductive component (107) is a conductive spring sheet; and further comprises a rotating member (108), the rotating member (108) being rotatably disposed at one end of the conductive spring sheet close to the power-on position, and at the power-on position, the first conductive component (106) abuts against the rotating member (108); The invention also comprises a rotating shaft (105), wherein the rotating shaft (105) is arranged at the rotation center of the first turntable (103) and extends toward the second base (109); a fixed shaft (1071) is arranged on a side wall of the conductive spring sheet facing the rotating shaft (105); the rotating member (108) is rotatably sleeved on the fixed shaft (1071); the conductive spring sheet is provided with an avoidance zone for the rotating member (108) to rotate; and the rotating member (108) is made of a conductive material.

2. The multi-band light source gating device according to claim 1, characterized in that: The rotor (1042) of the conductive slip ring is fixedly connected to the rotating shaft (105).

3. The multi-band light source gating device according to claim 2, characterized in that: The fixing assembly also includes an upper cover (111), which is arranged on the second base (109), and has a circular opening in the middle of the upper cover (111), and the bottom of the first turntable (103) is rotatably engaged in the circular opening; the first turntable (103) has a first flange (1031) on its outer periphery, and the bottom of the first flange (1031) abuts against the upper cover (111).

4. The multi-band light source gating device according to any one of claims 1 to 3, characterized in that: It also comprises a positioning component (110), wherein the positioning component (110) is arranged on the second base (109) in a telescopic manner upward and downward; after the first rotating disk (103) is rotated into position, the positioning component (110) abuts against the first rotating disk (103) to fix the first rotating disk (103).

5. The multi-band light source gating device according to claim 4, characterized in that: The positioning component (110) is a spring plunger.

6. A microscopic imaging system, characterized in that: A multi-band light source gating device comprising any one of claims 1-5.

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