A magnetic bead transfer clamping device

Abstract

A magnetic bead transfer clamping device includes a magnetic rod fixing plate, a magnetic rod, a clamping member mounting through hole, a driving mechanism, and a clamping mechanism. The driving mechanism includes a mounting rod, a spring, a movable plate, and a lifting driving rod. The movable plate includes a movable plate body, a groove-shaped through hole, and a mounting through hole. Each of the clamping mechanisms includes a first rotating shaft, a second rotating shaft, and a clamping member. The clamping member includes a rotation driving portion, a clamping plate, and a connecting rod. The rotation driving portion includes a rotation driving rod and a rotation mounting seat. The clamping plate includes a clamping plate body and a jack. When the driving mechanism is in the initial position, in the cross-section along the central axis of the clamping mechanism, the extending direction of the clamping plate is parallel to the magnetic rod fixing plate, so as to stably clamp the magnetic sleeve by the clamping plate. This magnetic bead transfer clamping device has a simple structure, is convenient for clamping, improves work efficiency, and reduces the processing cost of the magnetic sleeve.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological instrument equipment, and particularly relates to a magnetic bead transfer clamping device. Background Art

[0002] As a carrier of genetic information, nucleic acid is the material basis of gene expression. In addition to playing a very important role in the normal life activities of organisms such as growth, development, and reproduction, it is also closely related to abnormal conditions of life, such as tumor occurrence, radiation damage, genetic diseases, etc. Therefore, nucleic acid isolation and purification is a very important link in molecular biology and medical research. In addition, the separation and purification of proteins is also an important step in sample pretreatment in modern biomedical research.

[0003] Magnetic beads can selectively adsorb target molecules such as nucleic acids or proteins, and the target molecules are purified through vibration processing by machine equipment during the separation process. However, the structures of current nucleic acid and protein extractors are complex and require specific magnetic sleeves for clamping, which is not convenient to use and increases the cost of magnetic sleeves. Summary of the Invention

[0004] In view of this, the present invention provides a magnetic bead transfer clamping device that can be used independently or installed in an extractor and is convenient to use to meet industrial requirements.

[0005] A magnetic bead transfer clamping device includes a magnetic rod fixing plate, a plurality of magnetic rods arranged on one side of the magnetic rod fixing plate, a plurality of clamping member mounting through holes arranged on the magnetic rod fixing plate, a driving mechanism arranged on the side of the magnetic rod fixing plate facing away from the magnetic rods, and two clamping mechanisms symmetrically arranged on the driving mechanism and used for clamping a magnetic sleeve. The driving mechanism includes a plurality of mounting rods arranged on the magnetic rod fixing plate, a plurality of first return springs sleeved on the mounting rods, a movable plate slidably connected to the mounting rods, a lifting driving rod abutted against the side of the movable plate facing away from the mounting rods, a protective plate arranged on the movable plate, and two limiting plates symmetrically arranged on both sides of the movable plate. The outer contour of the limiting plate is Z-shaped. The protective plate includes a protective plate main body arranged on the movable plate and a driving rod mounting through hole arranged on the protective plate main body. The lifting driving rod includes a first driving rod inserted into the protective plate, a second driving rod arranged on the first driving rod, and a second return spring sleeved on the first driving rod. The movable plate includes a movable plate main body, a trough-shaped through hole arranged on the movable plate main body, and a plurality of mounting through holes arranged on the movable plate main body and used for inserting the mounting rods. The extending direction of the trough-shaped through hole is perpendicular to the extending direction of the central axis of the mounting through hole. Each clamping mechanism includes a first rotating shaft inserted into the trough-shaped through hole, a second rotating shaft arranged on the magnetic rod fixing plate, and at least one clamping member arranged on the first rotating shaft and the second rotating shaft. The central axis of the second rotating shaft is parallel to the central axis of the first rotating shaft. The clamping member includes a rotation driving part arranged on the first rotating shaft, a clamping plate arranged on the second rotating shaft, and a connecting rod arranged between the rotation driving part and the clamping plate and passing through the clamping member mounting through hole. The rotation driving part includes a rotation driving rod arranged on the first rotating shaft and a rotation mounting seat fixedly connected to the magnetic rod fixing plate and pin-connected to the rotation driving rod. The rotation mounting seat is pin-connected to a position close to the center of the rotation driving rod. The clamping plate includes a clamping plate main body and a jack arranged on one side of the clamping plate main body. The second rotating shaft passes through the jack. One end of the connecting rod is pin-connected to one end of the rotation driving rod, and the other end of the connecting rod is pin-connected to one end of the clamping plate main body. The rotation driving rod is inclinedly arranged.When the driving mechanism is in the initial position, in the cross-section along the central axis of the clamping mechanism, the rotating driving rod is inclined, and the distance between the end of the rotating driving rod close to the first rotating shaft and the magnetic rod fixing plate is greater than the distance between the end of the rotating driving rod far from the first rotating shaft and the magnetic rod fixing plate. The length of the slot-shaped through hole is greater than the diameters of the two first rotating shafts, and the extending direction of the clamping plate is parallel to the magnetic rod fixing plate, so that the clamping plate can stably clamp the magnetic sleeve.

[0006] Further, one side of the limiting plate abuts against the side of the protection plate facing away from the movable plate.

[0007] Further, the first driving rod is inserted into the driving rod installation through hole, and one end of the first driving rod abuts against one side of the movable plate, and the length of the first driving rod is greater than the height of the driving rod installation through hole.

[0008] Further, the diameter of the first driving rod is smaller than the diameter of the second driving rod, and the second return spring abuts against one end of the second driving rod.

[0009] Compared with the prior art, the bead transfer clamping device provided by the present invention drives the two clamping mechanisms to clamp the magnetic sleeve through the driving mechanism. The movable plate of the driving mechanism can move along the mounting rod, and the first return spring sleeved on the mounting rod can use its own elastic force to reset the movable plate. The first rotating shaft of each clamping mechanism is inserted into the slot-shaped through hole of the movable plate, the second rotating shaft is arranged on the magnetic rod fixing plate, and the clamping member is arranged on the first rotating shaft and the second rotating shaft. The clamping member includes a rotation driving part arranged on the first rotating shaft, a clamping plate arranged on the second rotating shaft, and a connecting rod arranged between the rotation driving part and the clamping plate and passing through the mounting through hole of the clamping member. One end of the rotation driving rod in the rotation driving part is connected to the first rotating shaft, and the other end of the rotation driving rod is connected to the connecting rod. The rotation mounting seat is pivotally connected to a position close to the center of the rotation driving rod, that is, the rotation driving rod can rotate around the rotation mounting seat. The second rotating shaft passes through the insertion hole of the clamping plate, so that the clamping plate can rotate around the second rotating shaft. When the driving mechanism is pressed down, the movable plate drives the first rotating shaft and one end of the rotation driving rod to move towards the side of the magnetic rod fixing plate, so that the rotation driving rod rotates around the rotation mounting seat. At the same time, the end of the rotation driving rod close to the connecting rod rises, and the connecting rod pulls one end of the clamping plate to rise. Thus, the clamping plate rotates around the second rotating shaft, and the free end of the clamping plate moves away from the side of the magnetic rod fixing plate. Therefore, when the clamping mechanism clamps the magnetic sleeve or places the magnetic sleeve on the clamping mechanism, the clamping plates of the two clamping mechanisms move away from each other, so that the clamping plates of the clamping mechanism are convenient to clamp or disengage from the edge of the magnetic sleeve. When the driving mechanism resets, the first return spring uses its own elasticity to return the driving mechanism to the initial position, and the extending direction of the clamping plate is parallel to the magnetic rod fixing plate, so as to stably clamp the magnetic sleeve by the clamping plate. The bead transfer clamping device has a simple structure, is convenient to clamp, improves the working efficiency, and reduces the processing cost of the magnetic sleeve. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 FIG. is a schematic structural diagram of the bead transfer clamping device provided by the present invention.

[0011] Figure 2 is Figure 1 exploded structural diagram of the bead transfer clamping device. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0012] The following further details the specific embodiments of the present invention. It should be understood that the description of the embodiments of the present invention herein is not intended to limit the protection scope of the present invention.

[0013] As shown Figures 1 to 2 in the figure, it is a schematic structural diagram of the magnetic bead transfer clamping device provided by the present invention. The magnetic bead transfer clamping device includes a magnetic rod fixing plate 1, a plurality of magnetic rods 2 arranged on one side of the magnetic rod fixing plate 1, a plurality of clamping member mounting through holes 3 arranged on the magnetic rod fixing plate 1, a driving mechanism 10 arranged on the side of the magnetic rod fixing plate 1 opposite to the magnetic rods 2, and two clamping mechanisms 20 symmetrically arranged on the driving mechanism 10 and used for clamping a magnetic sleeve 4. The magnetic bead transfer clamping device further includes some other functional modules, such as an assembly component, etc., which should be well-known technologies to those skilled in the art and will not be described in detail one by one here.

[0014] The magnetic rod fixing plate 1 is used to fix the magnetic rods 2, the magnetic rods 2 are used to adsorb iron-containing substances and other magnetizable substances, the clamping member mounting through holes 3 are used to pass through the clamping mechanisms 20 for clamping, and the magnetic rod fixing plate 1, the magnetic rods 2, and the clamping member mounting through holes 3 are all prior arts and will not be elaborated here.

[0015] The driving mechanism 10 includes a plurality of mounting rods 11 arranged on the magnetic rod fixing plate 1, a plurality of first return springs 12 sleeved on the mounting rods 11, a movable plate 13 slidably connected to the mounting rods 11, a lifting driving rod 14 abutted against the side of the movable plate 13 opposite to the mounting rods 11, a protective plate 15 arranged on the movable plate 13, and two limiting plates 16 symmetrically arranged on both sides of the movable plate 13.

[0016] The mounting rods 11 are used to mount the movable plate 13 and guide the movable plate 13 to slide along the mounting rods 11 to realize the lifting of the movable plate 13. In this embodiment, there are four mounting rods 11, and the four mounting rods 11 are respectively arranged at the top corner positions of the movable plate 13.

[0017] Each of the first return springs 12 is sleeved outside one of the mounting rods 11. When the movable plate 13 slides along the mounting rods 11, the first return springs 12 use their own elasticity to reset the movable plate 13.

[0018] The movable plate 13 includes a movable plate body 131, a slot-shaped through hole 132 provided on the movable plate body 131, and a plurality of mounting through holes 133 provided on the movable plate body 131 and used for inserting the mounting rod 11. The extending direction of the slot-shaped through hole 132 is perpendicular to the extending direction of the central axis of the mounting through hole 131. The mounting rod 11 is inserted into the mounting through hole 133, so that the mounting rod 11 can guide the movement of the movable plate 13, and the slot-shaped through hole 132 is used for arranging the clamping mechanism 20, and the slot-shaped through hole 132 will be described in detail in combination with the clamping mechanism 20.

[0019] The lifting drive rod 14 includes a first drive rod 141 inserted into the protective plate 15, a second drive rod 142 provided on the first drive rod 141, and a second return spring 143 sleeved on the first drive rod 141. The diameter of the first drive rod 141 is smaller than the diameter of the second drive rod 142. The second return spring 143 abuts against one end of the second drive rod 142. When the lifting drive rod 14 is installed on the protective plate 15, one end of the second return spring 143 abuts against the protective plate 15, so that after the lifting drive rod 14 is pressed down, the lifting drive rod 14 can automatically reset by using the second return spring 143.

[0020] One end of the lifting drive rod 14 abuts against one side end face of the movable plate 13, and the lifting drive rod 14 is connected to other devices (not shown in the figure), so as to drive the movable plate 13 to descend.

[0021] The protective plate 15 includes a protective plate body 151 provided on the movable plate 13 and a drive rod mounting through hole 152 provided on the protective plate body 151. The first drive rod 141 is inserted into the drive rod mounting through hole 152, and one end of the first drive rod 141 abuts against one side of the movable plate 13. The length of the first drive rod 141 is greater than the height of the drive rod mounting through hole 15, so that the lifting drive rod 14 can drive the movable plate 13 to move.

[0022] One side of the limiting plate 16 abuts against the side of the protective plate 15 facing away from the movable plate 13, so as to limit the positions of the protective plate 15 and the movable plate 13 and prevent the protective plate 15 and the movable plate 13 from falling off the mounting rod 11. In this embodiment, the outer contour of the limiting plate 16 is Z-shaped.

[0023] Each of the clamping mechanisms 20 includes a first rotating shaft 21 inserted into the through slot 132, a second rotating shaft 22 disposed on the magnet rod fixing plate 1, and at least one clamping member 23 disposed on the first rotating shaft 21 and the second rotating shaft 22.

[0024] Both the first rotating shaft 21 and the second rotating shaft 22 pass through the clamping member 23, so that the clamping member 23 can rotate around the first rotating shaft 21 and the second rotating shaft 22. The central axis of the second rotating shaft 22 is parallel to the central axis of the first rotating shaft 21, so that the following rotation driving portion 231 of the clamping member 23 rotates in the same direction as the following clamping plate 232. The first rotating shaft 21 and the second rotating shaft 22 will be described in detail in conjunction with the clamping member 23.

[0025] The clamping member 23 includes a rotation driving portion 231 disposed on the first rotating shaft 21, a clamping plate 232 disposed on the second rotating shaft 22, and a connecting rod 233 disposed between the rotation driving portion 231 and the clamping plate 232 and passing through the clamping member mounting through hole 3. The rotation driving portion 231 and the clamping plate 232 are respectively disposed on both sides of the magnet rod fixing plate 1, and the clamping plate 232 and the magnet rod 2 are both disposed on the same side surface of the magnet rod fixing plate 1.

[0026] The rotation driving portion 231 includes a rotation driving rod 2311 disposed on the first rotating shaft 21, and a rotation mounting seat 2312 fixedly connected to the magnet rod fixing plate 1 and pin-connected to the rotation driving rod 2311.

[0027] One end of the rotation driving rod 2311 is connected to the first rotating shaft 21, and the other end of the rotation driving rod 2311 is connected to the connecting rod 233. Thus, the driving mechanism 10 can drive the rotation driving rod 2311 to move, so that the rotation driving rod 2311 rotates around the rotation mounting seat 2312, and then drives the connecting rod 233 to move, so that the clamping plate 232 moves. The rotation driving rod 2311 is inclined to facilitate the driving of the rotation driving rod 2311 by the movable plate 13.

[0028] The rotation mounting seat 2312 is pin-connected to a position close to the center of the rotation driving rod 2311, that is, the rotation driving rod 2311 can rotate around the rotation mounting seat 2312.

[0029] The clamping plate 232 includes a clamping plate main body 2321 and a jack 2322 disposed on one side of the clamping plate main body 2321.

[0030] The second rotating shaft 22 passes through the jack 2322, so that the clamping plate 232 can rotate around the second rotating shaft 22.

[0031] The connecting rod 233 passes through the clamping member mounting through hole 3. One end of the connecting rod 233 is pin-connected to one end of the rotation driving rod 2311, and the other end of the connecting rod 233 is pin-connected to one end of the clamping plate main body 2321. The connection positions of the connecting rod 233 with the rotation driving rod 2311 and the clamping plate main body are all rotatable, and the rotation driving rod 2311 can drive the clamping plate 232 to rotate.

[0032] When the driving mechanism 10 is not pressed down, it is the initial position of the driving mechanism 10. When the driving mechanism 10 is in the initial position, on the cross-section along the central axis of the clamping mechanism 20, the rotation driving rod 2311 is inclined, and the distance between the end of the rotation driving rod 2311 close to the first rotating shaft 21 and the magnetic rod fixing plate 1 is greater than the distance between the end of the rotation driving rod 2311 far from the first rotating shaft 21 and the magnetic rod fixing plate 1. It can be imagined that the height of the end of the rotation driving rod 2311 close to the first rotating shaft 21 is greater than the height of the end of the rotation driving rod 2311 far from the first rotating shaft 21, so as to facilitate the movable plate 13 to drive the first rotating shaft 21 and one end of the rotation driving rod 2311 to move towards the magnetic rod fixing plate 1. And the extending direction of the clamping plate 232 is parallel to the magnetic rod fixing plate 1, so that the clamping plate 232 stably abuts against the edge of the magnetic sleeve 4, so that the clamping plate 232 clamps the magnetic sleeve 4 stably.

[0033] When the driving mechanism 10 presses down, the movable plate 13 drives the first rotating shaft 21 and one end of the rotating driving rod 2311 to move towards the side of the magnetic rod fixing plate 1. Since the length of the groove-shaped through hole 132 is greater than the diameters of the two first rotating shafts 21, the two first rotating shafts 21 on the two clamping mechanisms 20 approach each other, so as to drive one end of the rotating driving rod 2311 to move towards the side of the magnetic rod fixing plate 1, thereby causing the rotating driving rod 2311 to rotate around the rotating mounting seat 2312. At the same time, the end of the rotating driving rod 2311 close to the connecting rod 233 rises, and the connecting rod 233 pulls one end of the clamping plate 232 to rise. The clamping plate 232 rotates around the second rotating shaft 22, so that the free end of the clamping plate 232 moves away from the side of the magnetic rod fixing plate 1. That is, when the driving mechanism 10 presses down, the clamping plate 232 is in an inclined state. It can be imagined that when the clamping mechanism 20 clamps the magnetic sleeve 4 or places the magnetic sleeve 4 on the clamping mechanism 20, the clamping plates 232 of the two clamping mechanisms 20 move away from each other, so that the clamping plates 232 of the clamping mechanism 20 are convenient to clamp or disengage from the edge of the magnetic sleeve 4. When the driving mechanism 10 resets, the first return spring 12 uses its own elasticity, and the driving mechanism 10 returns to the initial position. Thus, the clamping plate 232 resets, and the extending direction of the clamping plate 232 is parallel to the magnetic rod fixing plate 1, so that the clamping plate 232 stably clamps the magnetic sleeve 4.

[0034] Compared with the prior art, the magnetic bead transfer clamping device provided by the present invention drives the two clamping mechanisms 20 to clamp the magnetic sleeve 4 through the driving mechanism 10. The movable plate 13 of the driving mechanism 10 can move along the mounting rod 11, and the first return spring 12 sleeved on the mounting rod 11 can use its own elastic force to reset the movable plate 13. The first rotating shaft 21 of each clamping mechanism 20 is inserted into the groove-shaped through hole 132 of the movable plate 13, the second rotating shaft 22 is arranged on the magnetic rod fixing plate 1, and the clamping member 23 is arranged on the first rotating shaft 21 and the second rotating shaft 22. The clamping member 23 includes a rotation driving portion 231 arranged on the first rotating shaft 21, a clamping plate 232 arranged on the second rotating shaft 22, and a connecting rod 233 arranged between the rotation driving portion 231 and the clamping plate 232 and passing through the clamping member mounting through hole 3. One end of the rotation driving rod 2311 in the rotation driving portion 231 is connected to the first rotating shaft 21, and the other end of the rotation driving rod 2311 is connected to the connecting rod 233. The rotation mounting seat 2312 is pinned at a position close to the center of the rotation driving rod 2311, that is, the rotation driving rod 2311 can rotate around the rotation mounting seat 2312. The second rotating shaft 22 passes through the insertion hole 2322 of the clamping plate 232, so that the clamping plate 232 can rotate around the second rotating shaft 22. When the driving mechanism 10 is pressed down, the movable plate 13 drives the first rotating shaft 21 and one end of the rotation driving rod 2311 to move towards the magnetic rod fixing plate 1 side, so that the rotation driving rod 2311 rotates around the rotation mounting seat 2312. At the same time, the end of the rotation driving rod 2311 close to the connecting rod 233 rises, and the connecting rod 233 pulls one end of the clamping plate 232 to rise. Thus, the clamping plate 232 rotates around the second rotating shaft 22, and the free end of the clamping plate 232 moves away from the magnetic rod fixing plate 1 side. Therefore, when the clamping mechanism 20 clamps the magnetic sleeve 4 or places the magnetic sleeve 4 on the clamping mechanism 20, the clamping plates 232 of the two clamping mechanisms 20 move away from each other, so that the clamping plates 232 of the clamping mechanism 20 are convenient to clamp or disengage from the edge of the magnetic sleeve 4. When the driving mechanism 10 is reset, the first return spring 12 uses its own elasticity to make the driving mechanism 10 return to the initial position, and the extending direction of the clamping plate 232 is parallel to the magnetic rod fixing plate 1 to make the clamping plate 232 clamp the magnetic sleeve 4 stably. The magnetic bead transfer clamping device has a simple structure, is convenient to clamp, improves the working efficiency, and reduces the processing cost of the magnetic sleeve.

[0035] The above are only the preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modifications, equivalent substitutions or improvements within the spirit of the present invention are all covered within the scope of the claims of the present invention.

Claims

1. A magnetic bead transfer clamping device, comprising a magnetic rod fixing plate, a plurality of magnetic rods arranged on one side of the magnetic rod fixing plate, and a plurality of clamping member mounting through holes arranged on the magnetic rod fixing plate, wherein: The magnetic bead transfer clamping device further includes a driving mechanism disposed on the side of the magnetic rod fixing plate facing away from the magnetic rod, two clamping mechanisms symmetrically disposed on the driving mechanism and used for clamping the magnetic sleeve. The driving mechanism includes a plurality of mounting rods disposed on the magnetic rod fixing plate, a plurality of first return springs sleeved on the mounting rods, a movable plate slidably connected to the mounting rods, a lifting driving rod abutted against the side of the movable plate facing away from the mounting rods, a protective plate disposed on the movable plate, and two limiting plates symmetrically disposed on both sides of the movable plate. The outer contour of the limiting plate is Z-shaped. The protective plate includes a protective plate body disposed on the movable plate and a driving rod mounting through hole disposed on the protective plate body. The lifting driving rod includes a first driving rod inserted into the protective plate, a second driving rod disposed on the first driving rod, and a second return spring sleeved on the first driving rod. The movable plate includes a movable plate body, a groove-shaped through hole disposed on the movable plate body, and a plurality of mounting through holes disposed on the movable plate body and used for inserting the mounting rods. The extending direction of the groove-shaped through hole is perpendicular to the extending direction of the central axis of the mounting through hole. Each clamping mechanism includes a first rotating shaft inserted into the groove-shaped through hole, a second rotating shaft disposed on the magnetic rod fixing plate, and at least one clamping member disposed on the first rotating shaft and the second rotating shaft. The central axis of the second rotating shaft is parallel to the central axis of the first rotating shaft. The clamping member includes a rotation driving portion disposed on the first rotating shaft, a clamping plate disposed on the second rotating shaft, and a connecting rod disposed between the rotation driving portion and the clamping plate and passing through the clamping member mounting through hole. The rotation driving portion includes a rotation driving rod disposed on the first rotating shaft and a rotation mounting seat fixedly connected to the magnetic rod fixing plate and pinned to the rotation driving rod. The rotation mounting seat is pinned at a position close to the central position of the rotation driving rod. The clamping plate includes a clamping plate body and a jack disposed on one side of the clamping plate body. The second rotating shaft passes through the jack. One end of the connecting rod is pinned to one end of the rotation driving rod, and the other end of the connecting rod is pinned to one end of the clamping plate body. The rotation driving rod is inclined. When the driving mechanism is in the initial position, in the cross-section along the central axis of the clamping mechanism, the rotation driving rod is inclined, and the distance between the end of the rotation driving rod close to the first rotating shaft and the magnetic rod fixing plate is greater than the distance between the end of the rotation driving rod away from the first rotating shaft and the magnetic rod fixing plate. The length of the groove-shaped through hole is greater than the diameters of the two first rotating shafts, and the extending direction of the clamping plate is parallel to the magnetic rod fixing plate, so that the magnetic sleeve can be stably clamped by the clamping plate.

2. The magnetic bead transfer clamping device according to claim 1, wherein: One side of the limiting plate abuts against the side of the protective plate facing away from the movable plate.

3. The magnetic bead transfer and clamping device according to claim 1, characterized in that: The first driving rod is inserted into the through hole for installing the driving rod, and one end of the first driving rod abuts against one side of the movable plate, and the length of the first driving rod is greater than the height of the through hole for installing the driving rod.

4. The magnetic bead transfer and clamping device according to claim 1, characterized in that: The diameter of the first driving rod is smaller than the diameter of the second driving rod, and the second return spring abuts against one end of the second driving rod.

Images