Salivary α-amylase detection device

Abstract

The present invention provides a salivary alpha amylase detection device, comprising a sampling member and a detection member both in the form of a plate, wherein the detection member and the sampling member are pivotally fixed in a detachable manner; a water absorbing member for collecting saliva to be detected is provided on one side of the sampling member facing the detection member, and a reaction member is provided on one side of the detection member facing the water absorbing member, wherein the reaction member comprises a reaction part made of an amylase reaction reagent, and when the detection member rotates to a preset position around the pivotal part, the reaction part contacts the water absorbing member. The present invention greatly improves the efficiency of saliva detection by providing the water absorbing member and the reaction member, and the saliva to be detected is collected by the water absorbing member in a dry manner, which is convenient to carry and operate, and the operation is simple and convenient, which is conducive to popularization and application, and can also effectively solve the problem of loss and confusion of saliva to be detected, and can be used in a field with a large number of detections, thereby improving the practicality of use.

Description

Technical Field

[0001] The embodiments of the present invention relate to the technical field of medical devices, and more specifically, to a salivary alpha-amylase detection device. Background Art

[0002] Stress is defined as a state in which the homeostasis of an organism is threatened by a potential adverse physiological or psychological state (real or perceived). Stress responses can damage the natural regulatory state of an organism. When the equilibrium state is disrupted and cannot be restored, the body may be damaged, which is the root cause of various diseases.

[0003] Stress is unavoidable. The body has evolved responses to stress aimed at maintaining healthy physiological functions, but frequent stress is detrimental to health. Stress leads to immune dysregulation, impaired cognitive abilities, and behaviors such as self-harm. Stress is associated with many diseases with a higher incidence in older age, such as cardiovascular disease, atherosclerosis, cancer, or type 2 diabetes. Aging is associated with several psychobiological changes, such as increased susceptibility to oxidative stress, imbalances in central neurotransmitter pathways, and changes in emotion regulation.

[0004] Stress initiates the stress response by activating the hypothalamic-pituitary-adrenal axis, which results in the release of glucocorticoids, and the sympathetic-adrenal-medullary axis, which results in the release of the catecholamines epinephrine and norepinephrine. Compared with the hypothalamic-pituitary-adrenal axis, the sympathetic-adrenal-medullary axis is initially more reactive to stress activation.

[0005] Stress hormones are essential for quantifying and tracking stress responses. To better understand the stress response system, it is necessary to expand the scope of research on physiological stress markers. The development of non-invasive methods is very useful in assessing stress responses.

[0006] Saliva testing is a non-invasive diagnostic tool for the person being tested and can help prevent, diagnose and treat a variety of diseases. Advantages and benefits of saliva analysis:

[0007] 1. As a test specimen, saliva has the advantages of being easy to collect and can be collected repeatedly, not being restricted by the environment and time, and being easy to operate.

[0008] 2. Saliva testing can reduce damage to the body and is non-traumatic, non-irritating and painless.

[0009] 3. Saliva testing can reduce testing conditions and costs, increase testing speed, and dynamically monitor indicators.

[0010] 4. Reduce the risk of contracting hepatitis B, HIV virus, etc. during the examination.

[0011] At present, the existing saliva testing methods mostly rely on sampling tubes, which collect saliva and then send the sampling tubes to the testing center for testing. The existing saliva testing methods are non-dry testing methods, and the test results cannot be directly obtained on site, which not only makes the test efficiency low, but also inconvenient to operate and carry, which is not conducive to the promotion and application of saliva testing.

[0012] In addition, since the existing saliva testing method relies on sampling tubes to collect saliva and cannot be directly tested, there are problems of loss and confusion, and its practicality is relatively insufficient. Summary of the invention

[0013] The embodiments of the present invention provide a salivary α-amylase detection device to address the problems that the existing saliva detection methods cannot directly obtain detection results on site, have low detection efficiency, are inconvenient to operate and carry, are not conducive to the promotion and application of saliva detection, and have the problem of loss and confusion.

[0014] The technical solution for solving the above technical problems in an embodiment of the present invention is to provide a salivary α-amylase detection device, comprising a sampling member and a detection member both in the shape of a plate, wherein the detection member and the sampling member are pivotally fixed in a detachable manner; a water absorption member for collecting saliva to be detected is provided on a side of the sampling member facing the detection member, and a reaction member is provided on a side of the detection member facing the water absorption member, wherein the reaction member comprises a reaction portion made of an amylase reaction reagent, and when the detection member rotates around the pivotal portion to a preset position, the reaction portion contacts the water absorption member.

[0015] Preferably, the detection component includes a first detection window, the reaction component includes a main body composed of a transparent material, and the reaction part is located on the side of the main body facing the sampling component; the reaction component covers the first detection window, and the first detection window is opposite to the reaction part, and the reaction part is optically detected through the first detection window.

[0016] Preferably, the sampling member includes a second detection window, the water absorbing member covers the second detection window, and when the reaction part contacts the water absorbing member, the second detection window is opposite to the reaction part, and the reaction part is optically detected through the first detection window and the second detection window.

[0017] Preferably, the salivary α-amylase detection device further comprises a flat protective member, and the protective member is located on a side of the sampling member facing away from the detection member and is symmetrically distributed with the detection member;

[0018] The protective component includes a third detection window, and the protective component is pivotally fixed to the sampling component in a detachable manner; when the protective component and the detection component are respectively buckled onto the sampling component, the water absorption component on the sampling component is located between the protective component and the detection component, and the third detection window is opposite to the second detection window.

[0019] Preferably, a C-shaped first elastic buckle is provided in the middle of the sampling member, the first elastic buckle is arranged along the width direction of the sampling member, and the sampling member is divided into a handle section and a sampling section by the first elastic buckle, and the water absorbing member is arranged in the sampling section;

[0020] A first pivot shaft adapted to the first elastic buckle is provided at one end of the detection member, the first pivot shaft is perpendicular to the length direction of the detection member, and the detection member is pivotally fixed to the sampling member by the first pivot shaft being snapped into the first elastic buckle;

[0021] A second elastic buckle in a C shape is provided on the side of the sampling component facing away from the first elastic buckle, and the opening direction of the second elastic buckle is opposite to the opening direction of the first elastic buckle; a second pivot axis adapted to the second elastic buckle is provided at one end of the protective component, the second pivot axis is perpendicular to the length direction of the protective component, and the protective component is pivotally fixed to the sampling component by means of the second pivot axis being snapped into the second elastic buckle.

[0022] Preferably, the reaction member comprises a hydrophobic portion, the hydrophobic portion is located on a side of the main body facing the sampling member, and the reaction portion is made of an amylase reaction reagent through a vacuum drying process;

[0023] The hydrophobic portion surrounds the outer periphery of the reaction portion, and the hydrophobic portion is formed by molding a waterproof material onto the surface of the main body through laser printing technology.

[0024] Preferably, the outer edge of the detection member facing the sampling member is provided with a first clamping edge structure, the outer edge of the sampling member is provided with a first clamping groove structure adapted to the first clamping edge structure, and when the detection member is buckled onto the sampling member, the first clamping edge structure is clamped into the first clamping groove structure in a tight fit manner, so as to limit and fix the detection member on the sampling member;

[0025] The outer edge of the protective member facing the sampling member is provided with a second clamping edge structure, and the outer edge of the sampling member is provided with a second clamping groove structure adapted to the second clamping edge structure, and when the protective member is buckled onto the sampling member, the second clamping edge structure is clamped into the second clamping groove structure in a tight fit manner, so as to limit and fix the protective member on the sampling member;

[0026] When the protective component and the detection component are respectively buckled onto the sampling component, the sampling section of the sampling component is located in a cavity enclosed by the protective component and the detection component.

[0027] Preferably, the thickness of the water absorbing member is 0.2-0.5 mm, and the water absorption capacity of the water absorbing member is 5-20 ul / cm 2 , and the water absorbing component is bonded and fixed to the sampling component through an adhesive layer; the water absorbing component is composed of glass cellulose, quantitative filter paper, or cellulose acetate.

[0028] Preferably, the reaction member is in the shape of a rectangular sheet, and the thickness of the reaction member is 0.1-0.5 mm; the main body of the reaction member is made of polyethylene terephthalate, polymethyl methacrylate, polystyrene or polycarbonate;

[0029] The detection member is provided with an assembly groove matched with the reaction member on one side facing the sampling member, and the reaction member is fixed to the assembly groove by an adhesive layer; when the reaction member is assembled into the assembly groove, the reaction part of the reaction member protrudes from the opening plane of the assembly groove.

[0030] Preferably, the first detection window, the second detection window and the third detection window are respectively composed of a plurality of through holes arranged along the length direction of the salivary alpha-amylase detection device.

[0031] The salivary α-amylase detection device of the embodiment of the present invention has the following beneficial effects: by arranging a water absorbing member on the sampling member and arranging a reaction member on the detection member, when the detection member rotates around the rotating part to a preset position, the reaction part of the reaction member contacts the water absorbing member, that is, the saliva to be detected is collected by the water absorbing member, and the reaction part of the reaction member contacts the saliva to be detected to carry out a chemical reaction, and the reaction part generates a color to reflect the content of amylase in the saliva to be detected. At this time, it is only necessary to observe the color of the reaction part of the reaction member through an external detection device to obtain the content data of amylase in the saliva to be detected and complete the saliva detection, so that the detection result can be directly obtained on-site after the saliva is collected, which greatly improves the efficiency of saliva detection, and the saliva to be detected is collected by the water absorbing member, which is dry collection, easy to carry and operate, and the operation is simple and convenient, which is conducive to promotion and application; since the above-mentioned detection device collects the saliva to be detected by the water absorbing member and can be directly detected on-site, the problem of loss and confusion of the saliva to be detected is effectively solved, and it can be used in a field with a large number of detections, thereby improving the practicality of use. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 Schematic diagram of the structure of a salivary alpha-amylase detection device provided in an embodiment of the present invention;

[0033] Figure 2 is a schematic diagram of the structure of a lateral projection of a salivary alpha-amylase detection device provided in an embodiment of the present invention;

[0034] Figure 3 is a schematic structural diagram of a reaction component provided by an embodiment of the present invention;

[0035] Figure 4 It is a schematic diagram of the vertical projection structure of the salivary alpha-amylase detection device provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0037] like Figure 1 FIG. 1 is a schematic diagram of the structure of a salivary α-amylase detection device provided by an embodiment of the present invention, and the salivary α-amylase detection device can be applied to the field of medical device technology. Figure 2As shown, the salivary α-amylase detection device in this embodiment includes a sampling member 1 and a detection member 2 both in the form of a flat plate, and the detection member 2 and the sampling member 1 are pivotally fixed in a detachable manner, so that the detection member 2 can rotate relative to the sampling member 1 to avoid hindering the sampling operation of the sampling member 1. In practical applications, the sampling member 1 and the detection member 2 are preferably in the form of a long flat plate to facilitate sampling and storage operations.

[0038] The hinge structure between the detection component 2 and the sampling component 1 can be determined according to the actual situation; for example, the detection component 2 is assembled with the sampling component 1 by means of a snap connection, which is convenient for simplifying the structural design and is conducive to realizing a detachable fixed connection. In addition, the detection component 2 and the sampling component 1 are connected in a detachable manner, which is conducive to the processing and assembly of the detection component 2 and the sampling component 1.

[0039] Specifically, the sampling member 1 is provided with a water absorbent member 3 (such as absorbent paper) for collecting saliva to be tested on one side thereof facing the detection member 2. The water absorbent member 3 is preferably arranged near the end of the sampling member 1 so that the water absorbent member 3 can be placed under the tongue when the sampling member 1 is inserted into the oral cavity, thereby completing the collection of saliva.

[0040] Combination Figure 3 As shown, a reaction member 4 is provided on the side of the detection member 2 facing the water absorbing member 3. The reaction member 4 includes a reaction portion 41 made of an amylase reaction reagent (i.e., a detection reagent for amylase detection). The size of the reaction portion 41 can be determined according to actual conditions, and when the detection member 2 rotates around the pivot portion to a preset position, the reaction portion 41 contacts the water absorbing member 3. Since the reaction portion 41 is made of an amylase reaction reagent, and the saliva to be detected in the water absorbing member 3 contains amylase, the reaction portion 41 will undergo a chemical reaction under the catalytic action of amylase, thereby generating color. At this time, it is only necessary to obtain the color depth of the reaction portion 41 to obtain the activity and content of the salivary amylase in the saliva to be detected, thereby achieving the detection function of the saliva.

[0041] The preset position can be determined according to actual conditions, and is preferably the position where the detection member 2 is buckled onto the sampling member 1, which is conducive to improving the convenience of operation. The pivoting portion is specifically a structure where the detection member 2 and the sampling member 1 are pivotally connected.

[0042] The specific use steps are as follows: open and rotate the detection member 2, make the detection member 2 away from the water absorption member 3, place the part of the sampling member 1 provided with the water absorption member 3 under the tongue (the specific position can be adjusted according to the actual situation), wait for the water absorption member 3 to collect enough saliva to be detected, take out the sampling member 1, then fold the detection member 2, make the reaction member 4 contact with the water absorption member 3, and after standing for a certain period of time, perform optical detection (light reflection detection and light perspective detection) on the reaction part 41 of the reaction member 4 through an external detection device (such as a biological test paper detection device), obtain the color depth of the reaction part 41, and finally obtain the content of amylase in the saliva to be detected through an algorithm. Of course, the salivary α-amylase detection device of this embodiment can also be applied to the saliva detection of other salivary biomarkers (such as dogs), not limited to the saliva detection of human body.

[0043] The above-mentioned salivary α-amylase detection device is provided with a water absorbing member 3 on the sampling member 1 and a reaction member 4 on the detection member 2. Therefore, when the detection member 2 rotates around the rotating part to a preset position, the reaction part 41 of the reaction member 4 contacts the water absorbing member 3, that is, the water absorbing member 3 collects the saliva to be detected, and the reaction part 41 of the reaction member 4 contacts the saliva to be detected to carry out a chemical reaction, and the reaction part 41 generates a color to reflect the content of amylase in the saliva to be detected. At this time, it is only necessary to observe the color of the reaction part 41 of the reaction member 4 through an external detection device to obtain the content data of amylase in the saliva to be detected and complete the saliva detection, so that the detection result can be directly obtained on site after the saliva is collected, which greatly improves the efficiency of saliva detection.

[0044] In addition, the salivary α-amylase detection device collects the saliva to be detected by the water absorbing member 3, which is a dry collection method, easy to carry and operate, and the operation is simple and convenient, which is conducive to promotion and application. In addition, since the salivary α-amylase detection device collects the saliva to be detected by the water absorbing member 3 and can be directly detected on site, the problem of loss and confusion of the saliva to be detected is effectively solved, and it can be used in a large-scale detection field, thereby improving the practicality of use.

[0045] In one embodiment of the present invention, the detection member 2 includes a first detection window 21, the reaction member 4 includes a main body made of a transparent material (preferably polyethylene terephthalate), and the reaction part 41 is located on the side of the main body facing the sampling member 1 to ensure that the reaction part 41 can reliably contact the water absorbing member 3 to avoid contact failure caused by misalignment. In addition, the reaction member 4 covers the first detection window 21 of the detection member 2, and the first detection window 21 is opposite to the reaction part 41 of the reaction member 4. Since the main body of the reaction member 4 is transparent, the reaction part 41 can be optically detected through the first detection window 21, that is, the external detection equipment can optically detect the reaction part 41 of the reaction member 4 through the first detection window 21 to obtain the color depth of the reaction part 41.

[0046] In practical applications, the first detection window 21 may be specifically arranged according to the light source of the external detection device; for example, it may be composed of a plurality of through holes arranged along a straight line to correspond to a plurality of light sources on the external detection device to achieve optical detection of reflection and / or transmission.

[0047] In order to facilitate the optical perspective detection of the reaction part 41 of the reaction member 4, the sampling member 1 is provided with a second detection window 11, and the water absorbing member 3 covers the second detection window 11, and when the reaction part 41 contacts the water absorbing member 3, the second detection window 11 is opposite to the reaction part 41, thereby the reaction part 41 can be optically detected by reflection and / or transmission through the first detection window 21 and the second detection window 11. Of course, the specific detection method of the reaction part 41 can be determined according to the actual situation. In addition, when the detection member 2 rotates around the pivot portion to a preset position, it is preferred that the first detection window 21 and the second detection window 11 are located on the same straight line.

[0048] The structural design of the second detection window 11 may be the same as that of the first detection window 21; for example, it may be composed of a plurality of through holes arranged along a straight line, which facilitates a unified structure, simplifies the structural design, and ensures compatibility with the same external detection equipment.

[0049] In another embodiment of the present invention, the salivary α-amylase detection device further includes a flat protective member 5, and the protective member 5 is located on the side of the sampling member 1 facing away from the detection member 2, and is symmetrically distributed with the detection member 2, that is, the protective member 5 is located on the side of the water absorbing member 3 facing away from the detection member 2, so that the water absorbing member 3 can be enclosed and wrapped by the protective member 5 and the detection member 2, thereby improving the reliability of the detection.

[0050] Furthermore, the protective member 5 includes a third detection window 51, and the protective member 5 is pivotally fixed to the sampling member 1 in a detachable manner. When the protective member 5 and the detection member 2 are respectively buckled onto the sampling member 1, the water absorbing member 3 on the sampling member 1 is located between the protective member 5 and the detection member 2, and the third detection window 51 is opposite to the second detection window 11, so as to prevent the existence of the protective member 5 from blocking the light perspective detection of the external detection equipment. In particular, when the detection member 2 and the protective member 5 are rotatably buckled onto the sampling member 1, the first detection window 21, the second detection window 11 and the third detection window 51 are preferably located on the same straight line. Furthermore, the structural design of the third detection window 51 may also be the same as that of the first detection window 21; for example, it is composed of a plurality of through holes arranged along a straight line.

[0051] In practical applications, the sampling component 1 , the protective component 5 and the detection component 2 are preferably made of black opaque materials (such as plastic, rubber), which is conducive to external detection equipment to perform optical detection of the reaction part 41 through the first detection window 21 , the second detection window 11 and the third detection window 51 .

[0052] In one embodiment of the present invention, a C-shaped first elastic buckle 12 is provided in the middle of the sampling member 1, and the first elastic buckle 12 is arranged along the width direction of the sampling member 1, and the sampling member 1 is separated by the first elastic buckle 12 to form a handle section 13 and a sampling section 14, and the water absorbing member 3 is arranged in the sampling section 14. The sampling member 1 is convenient for carrying and hand-held operation by forming the handle section 13, and the stability during operation is improved. Of course, an anti-slip structure (such as a pattern) can be provided on the handle section 13, or the shape of the handle section 13 can be adapted to the palm, so as to improve the hand-held stability, so that the salivary α-amylase detection device is not easy to fall, and waste is avoided. The first elastic buckle 12 is preferably made of plastic or plastic material, which has low processing difficulty and low material cost, and is conducive to batch manufacturing and controlling manufacturing costs. Of course, the first elastic buckle 12 can also be made of other materials, such as stainless steel, which is determined according to actual conditions.

[0053] Furthermore, one end of the detection member 2 is provided with a first pivot shaft 22 adapted to the first elastic buckle 12, the first pivot shaft 22 is perpendicular to the length direction of the detection member 2, and the detection member 2 is pivotally fixed to the sampling member 1 by snapping the first pivot shaft 22 into the first elastic buckle 12. Since the first elastic buckle 12 is C-shaped, it can be ensured that the first elastic buckle 12 has good elasticity, which is conducive to the first pivot shaft 22 snapping into the first elastic buckle 12, that is, during installation, the first elastic buckle 12 can be elastically deformed by squeezing, and then snapped into the snap position of the first elastic buckle 12, thereby realizing the detachable connection between the detection member 2 and the sampling member 1, and ensuring the pivotal fixation of the detection member 2 and the sampling member 1, so that the detection member 2 can rotate relative to the sampling member 1 around the pivot portion (i.e., the first pivot shaft 22).

[0054] Since the first elastic buckle 12 is arranged along the width direction of the sampling member 1 and the first pivot axis 22 is perpendicular to the length direction of the detection member 2 (i.e. the width direction of the detection member 2), the detection member 2 is pivotally fixed to the sampling member 1 by means of the first pivot axis 22 being matched with the first elastic buckle 12. This can prevent the detection member 2 from rotating around the first pivot axis 22 and protruding toward the width direction of the sampling member 1, thereby improving the rationality of the structural design and also preventing the detection member 2 from rotating toward the width direction of the sampling member 1 and hindering the sampling operation of the sampling member 1 (i.e. the collection operation of the saliva to be tested), or causing scratches or scratches.

[0055] Similarly, a second elastic buckle 15 in a C shape is provided on the side of the sampling member 1 facing away from the first elastic buckle 12, and the opening direction of the second elastic buckle 15 is opposite to the opening direction of the first elastic buckle 12. A second pivot shaft 52 adapted to the second elastic buckle 15 is provided at one end of the protective member 5, and the second pivot shaft 52 is perpendicular to the length direction of the protective member 5, and the protective member 5 is pivotally fixed to the sampling member 1 by snapping the second pivot shaft 52 into the second elastic buckle 15. The pivoting method of the above-mentioned protective member 5 and the sampling member 1 can be the same as the pivoting method of the detection member 2 and the sampling member 1, which will not be repeated here.

[0056] In another embodiment of the present invention, the reaction member 4 includes a hydrophobic portion 42, which is located on the side of the main body facing the sampling member 1, and the reaction portion 41 of the reaction member 4 is made of an amylase reaction reagent through a vacuum drying process. In addition, the hydrophobic portion 42 surrounds the periphery of the reaction portion 41, and the hydrophobic portion 42 is formed by a waterproof material (preferably waterproof ink) formed on the surface of the main body through laser printing technology to form a hydrophobic barrier, which plays a hydrophobic role. Since the hydrophobic portion 42 surrounds the periphery of the reaction portion 41, the saliva to be detected on the water-absorbing member 3 can be more easily contacted with the reaction portion 41, ensuring that the reaction portion 41 can react more thoroughly with the amylase in the saliva to be detected, making the detection more accurate.

[0057] In addition, the outer edge of the detection member 2 facing the sampling member 1 is provided with a first clamping edge structure 23, and the outer edge of the sampling member 1 is provided with a first clamping groove structure 16 matched with the first clamping edge structure 23, and when the detection member 2 is buckled onto the sampling member 1, the first clamping edge structure 23 is clamped into the first clamping groove structure 16 in a tight-fitting manner to limit and fix the detection member 2 on the sampling member 1. The detection member 2 is clamped to the sampling member 1 in a tight-fitting manner, which facilitates the disassembly of the detection member 2 and the locking of the detection member 2 to the sampling member 1, improves the convenience of operation, and can simplify the structure to avoid complicating the structure and increasing the manufacturing cost.

[0058] Similarly, a second clamping structure 53 is provided on the outer edge of the protective component 5 facing the sampling component 1, and a second clamping groove structure 17 adapted to the second clamping structure 53 is provided on the outer edge of the sampling component 1. When the protective component 5 is buckled onto the sampling component 1, the second clamping structure 53 is clamped into the second clamping groove structure 17 in a tight fit so as to limit and fix the protective component 5 on the sampling component 1.

[0059] In practical applications, the connection between the detection component 2 and the sampling component 1, and the connection between the protective component 5 and the sampling component 1 can be specifically determined according to actual conditions; for example, the detection component 2 and the protective component 5 are provided with a card slot structure, and the sampling component 1 is provided with a card edge structure, and the detection component 2 and the protective component 5 are both locked to the sampling component 1 by means of a tight fit connection between the card slot structure and the card edge structure.

[0060] In particular, when the protective member 5 and the detection member 2 are respectively buckled onto the sampling member 1, the sampling section 14 of the sampling member 1 is located in the cavity enclosed by the protective member 5 and the detection member 2, so that the water absorption member 3 is located in the cavity, thereby protecting the water absorption member 3 and preventing the water absorption member 3 from contacting with the external air and absorbing moisture in the air, thereby avoiding affecting the concentration of saliva to be detected, thereby ensuring the detection accuracy.

[0061] In one embodiment of the present invention, the thickness of the water absorbing member 3 is 0.2-0.5 mm (the thickness of the water absorbing member 3 is preferably 0.3 mm), and the water absorption capacity of the water absorbing member 3 is 5-20 ul / cm 2 (The water absorption amount of the water absorbing member 3 is preferably 15 ul / cm 2 ), and the water absorbing member 3 can be specifically fixed to the sampling member 1 by an adhesive layer (such as a double-sided adhesive). The water absorbing member 3 can be a water absorbing paper, and the material is preferably glass cellulose. Of course, in practical applications, the material of the water absorbing member 3 can also be quantitative filter paper or cellulose acetate, which can be determined according to actual conditions.

[0062] In another embodiment of the present invention, the reaction member 4 is in a rectangular sheet shape, and the thickness of the reaction member 4 is 0.1-0.5 mm (the thickness of the reaction member 4 is preferably 0.2 mm). In particular, the main body of the reaction member 4 can also be made of polymethyl methacrylate, polystyrene or polycarbonate.

[0063] In addition, a mounting groove 24 is provided on one side of the detection member 2 facing the sampling member 1, and the reaction member 4 is fixed to the mounting groove 24 by bonding (e.g., applying glue) with an adhesive layer. The shape and size of the mounting groove 24 are preferably matched with the shape and size of the reaction member 4, so that the reaction member 4 can be limited by the mounting groove 24, which can reduce the difficulty of installing the reaction member 4, and the reaction member 4 can be positioned by the mounting groove 24, thereby improving the position accuracy of the reaction member 4 and avoiding displacement.

[0064] To ensure that the reaction portion 41 of the reaction member 4 can reliably contact the water absorbing member 3 , when the reaction member 4 is assembled into the assembly groove 24 , the reaction portion 41 of the reaction member 4 should protrude from the plane where the opening of the assembly groove 24 is located.

[0065] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A salivary α-amylase detection device, characterized in that: The invention comprises a sampling member, a detection member and a protective member, all of which are in the shape of a plate. The detection member, the protective member and the sampling member are pivotally fixed in a detachable manner; a water absorbing member for collecting saliva to be detected is provided on a side of the sampling member facing the detection member; a reaction member is provided on a side of the detection member facing the water absorbing member, the reaction member comprises a reaction part made of an amylase reaction reagent, and when the detection member rotates around the pivotal part to a preset position, the reaction part contacts the water absorbing member to trigger a reaction; the protective member is located on a side of the sampling member facing away from the detection member and is symmetrically distributed with the detection member; the detection member, the sampling member and the protective member are all provided with detection members. The detection window comprises a plurality of detection positions, each of which has a mutual visual field between the components; the detection component comprises a first detection window, the reaction component comprises a main body made of a transparent material, and the reaction part is located on the side of the main body facing the sampling component; the reaction component covers the first detection window, and the first detection window is opposite to the reaction part, and the reaction part is optically detected through the first detection window; the sampling component comprises a second detection window, the water absorbing component covers the second detection window, and when the reaction part contacts the water absorbing component, the second detection window is opposite to the reaction part, and the reaction part is optically detected through the first detection window and the second detection window; the protective component is a flat plate with a protective enclosure; the protective component comprises a third detection window, and the protective component is pivotally fixed to the sampling component in a detachable manner; when the protective component and the detection component are respectively buckled onto the sampling component, the water absorbing component on the sampling component is located between the enclosure formed by the protective component and the detection component, and the third detection window is opposite to the second detection window.

2. The salivary α-amylase detection device according to claim 1, characterized in that: A first elastic buckle in the shape of a letter C is provided in the middle of the sampling member, the first elastic buckle is arranged along the width direction of the sampling member, and the sampling member is divided into a handle section and a sampling section by the first elastic buckle, and the water absorbing member is arranged in the sampling section; a first pivot axis adapted to the first elastic buckle is provided at one end of the detection member, the first pivot axis is perpendicular to the length direction of the detection member, and the detection member is pivotally fixed to the sampling member by the first pivot axis being snapped into the first elastic buckle; a second elastic buckle in the shape of a letter C is provided on the side of the sampling member facing away from the first elastic buckle, and the opening direction of the second elastic buckle is opposite to the opening direction of the first elastic buckle; a second pivot axis adapted to the second elastic buckle is provided at one end of the protective member, the second pivot axis is perpendicular to the length direction of the protective member, and the protective member is pivotally fixed to the sampling member by the second pivot axis being snapped into the second elastic buckle.

3. The salivary α-amylase detection device according to claim 1, characterized in that: The reaction component includes a hydrophobic portion, which is located on the side of the main body facing the sampling component, and the reaction portion is made of amylase reaction reagent through a vacuum drying process; the hydrophobic portion surrounds the periphery of the reaction portion, and the hydrophobic portion is formed by waterproof material molded onto the surface of the main body through laser printing technology.

4. The salivary α-amylase detection device according to claim 2, characterized in that: The outer edge of the detection member facing the sampling member is provided with a first clamping edge structure, the outer edge of the sampling member is provided with a first clamping groove structure matched with the first clamping edge structure, and when the detection member is buckled onto the sampling member, the first clamping edge structure is clamped into the first clamping groove structure in a tight-fitting manner to limit and fix the detection member on the sampling member; the outer edge of the protective member facing the sampling member is provided with a second clamping edge structure, the outer edge of the sampling member is provided with a second clamping groove structure matched with the second clamping edge structure, and when the protective member is buckled onto the sampling member, the second clamping edge structure is clamped into the second clamping groove structure in a tight-fitting manner to limit and fix the protective member on the sampling member; when the protective member and the detection member are respectively buckled onto the sampling member, the sampling section of the sampling member is located in the cavity enclosed by the protective member and the detection member.

5. The salivary α-amylase detection device according to claim 1, characterized in that: The thickness of the water absorbing member is 0.2-0.5 mm, and the water absorption capacity of the water absorbing member is 5-20 μl / cm 2 , and the water absorbing component is bonded and fixed to the sampling component through an adhesive layer; the water absorbing component is composed of glass cellulose, quantitative filter paper, or cellulose acetate.

6. The salivary α-amylase detection device according to claim 1, characterized in that: The reaction member is in the shape of a rectangular sheet, and the thickness of the reaction member is 0.1 to 0.5 mm; the main body of the reaction member is made of polyethylene terephthalate, polymethyl methacrylate, polystyrene or polycarbonate; the detection member is provided with an assembly groove matched with the reaction member on the side facing the sampling member, and the reaction member is bonded and fixed to the assembly groove by an adhesive layer; when the reaction member is assembled into the assembly groove, the reaction part of the reaction member protrudes from the opening plane of the assembly groove.

7. The salivary α-amylase detection device according to claim 3, characterized in that: The first detection window, the second detection window, and the third detection window are respectively composed of a plurality of detection positions arranged along the length direction of the salivary α-amylase detection device; and the fields of view of the corresponding detection positions in each detection window are interconnected.

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