A traditional Chinese medicine hair growth and development composition and its preparation

Abstract

The present invention discloses a Chinese herbal medicine composition and preparation for hair growth. The composition primarily contains the following ingredients: Sanguisorba officinalis, Platycladus orientalis leaves, dried ginger, loquat leaves, ginkgo leaves, dandelion, and liquorice. The composition is subjected to a specific extraction process to obtain a corresponding extract, which exhibits excellent antibacterial and anti-inflammatory effects. This composition, based on carbomer as a matrix, 60% alcohol as a solvent, and glycerin, propylene glycol, azone, etc., is added to prepare a topical hydrogel. When applied topically to the scalp, the hydrogel exhibits anti-inflammatory, antibacterial, blood-activating, and hair follicle-nourishing effects. By increasing the number and activity of hair follicles, it increases the number and density of hair, achieving the desired effect of hair growth and development.

Description

Technical Field

[0001] The present invention belongs to the field of hairdressing and beauty, and in particular relates to a traditional Chinese medicine composition for hair growth and development and a preparation thereof. Background Art

[0002] Hair style and length are crucial to our lives today. However, with the development of society and the increasing pressures of life, the number of people suffering from hair loss is increasing, and the incidence is trending younger, significantly impacting their quality of life. According to literature, the incidence of hair loss is as high as 30.2% and is on the rise. Its complex pathology and long course remain a major medical challenge.

[0003] Hair grows in hair follicles, which have a cyclical growth pattern and can be divided into the growth phase, the regression phase, and the resting phase. Normal hair loss is mainly due to the hair follicles being in the regression phase and the resting phase, but the hair follicles in each phase are in a dynamic balance. For patients with hair loss, their hair follicles atrophy and cannot enter the growth phase, which causes the hair to gradually become sparse.

[0004] Currently, there are many Chinese and Western medicines for treating hair loss. Western medicines approved by the FDA include minoxidil and finasteride, but they both have shortcomings. Minoxidil is prone to relapse after discontinuation, and long-term use of finasteride can cause damage to the reproductive system. There are many Chinese herbal medicines for treating hair loss, but the effects are often not ideal, and they are expensive and the treatment course is extremely long. Therefore, it is very valuable to find a hair growth agent with easy-to-obtain materials, easy to use, significant efficacy and no rebound.

[0005] Traditional Chinese medicine has been used to treat hair loss since ancient times, and there are many commonly used Chinese herbal medicines for hair loss. Experimental studies have shown that some Chinese herbal medicines have good hair growth effects, which are introduced as follows:

[0006] Sanguisorba officinalis, first mentioned in Shennong's Herbal Classic, belongs to the Rosaceae family. Its slightly cold nature, bitter, astringent, and sour, has the effects of cooling the blood and stopping bleeding. Modern medical research has shown that Sanguisorba officinalis is rich in flavonoids and gallic acid, which have excellent antibacterial and anti-inflammatory properties.

[0007] Dried ginger, a plant in the Zingiberaceae family, has a long history of medicinal use in China. Traditional Chinese Medicine (TCM) theory states that it is pungent and warming, and enters the spleen, stomach, kidney, heart, and lung meridians, warming the middle and dispelling cold. Modern medical research has shown that dried ginger can increase blood pressure and act as an anticoagulant. The gingerol in dried ginger can activate transdermal capillaries, promote transdermal microcirculation, and enhance hair follicle nutrition.

[0008] Platycladus orientalis leaves are the dried branches and leaves of Platycladus orientalis, a plant of the Cupressaceae family. Traditional Chinese medicine believes that they are bitter and cold in nature, and have the effect of cooling blood and stopping bleeding. Modern medical research has shown that the flavonoids in Platycladus orientalis leaves have significant antibacterial and anti-inflammatory effects.

[0009] Loquat leaves, the dried leaves of the loquat plant, belong to the genus Pipa in the Rosaceae family. They are a commonly used traditional Chinese medicine. Traditional Chinese medicine theory suggests that loquat leaves have lung-clearing, cough-relieving, and anti-vomiting properties, and are particularly effective for various lung-heat coughs. Modern research has shown that loquat leaves are rich in triterpenoid acids, flavonoids, sesquiterpenes, and glycosides. Japanese researchers have also discovered that triterpenoid acids possess anti-cancer activity.

[0010] Ginkgo biloba, the dried leaves of the Ginkgo biloba plant, are used in Traditional Chinese Medicine to treat coughs, various heart diseases, hypertension, and hyperlipidemia. Ginkgo biloba extract is primarily composed of flavonoids, and modern research has shown that esters and other compounds in ginkgo biloba can scavenge free radicals, resist cell aging, and promote vascular microcirculation.

[0011] Dandelion, a plant of the Asteraceae family, tastes bitter, sweet, and is cold in nature. It has the effects of clearing away heat, detoxifying, and removing swelling and blood stasis. Modern medical research has shown that dandelion is rich in iron, calcium, and other minerals, which can effectively promote the nutrition of hair follicles.

[0012] Licorice, also known as national grass and sweet grass, is a perennial herb in the Leguminosae family. It has strong tonic properties, clearing away heat and toxins, and relieving coughs and expectoration. It is widely used in Traditional Chinese Medicine. The glycyrrhizic acid in it has anti-inflammatory and anti-allergic effects.

[0013] Chinese patent CN201810728202.6 discloses a traditional Chinese medicine composition and preparation for hair growth and protection, containing the medicinal herbs Sanguisorba officinalis, Taraxacum officinale, Ginkgo biloba, and Swertia japonica. The extract, prepared using a 95% alcohol extraction method, is effective in treating seborrheic alopecia, but its hair growth efficacy is suboptimal.

[0014] Chinese patent CN201710076200.9 discloses a traditional Chinese medicine composition for treating hair loss and its preparation method, wherein the medicinal materials are mainly angelica, emperor emperor, ligusticum chuanxiong, mulberry, astragalus, black sesame, mulberry, processed Polygonum multiflorum, Ligustrum lucidum fruit, and Platycladus orientalis leaves. The various medicines produce a synergistic effect to promote hair growth. The disadvantage is that the ratio of the medicinal materials is cumbersome, and the preparation process is complicated and time-consuming. Summary of the Invention

[0015] In view of the deficiencies of the existing technology, the present invention further optimizes the aforementioned Chinese herbal hair growth compound with Swertia japonica, Ginkgo biloba and Taraxacum mongolicum as main ingredients, and screens through a large number of experiments to obtain a Chinese herbal hair growth compound with easily available raw materials, more significant effects and more convenient use.

[0016] To achieve the above object, the present invention provides the following technical solutions:

[0017] A traditional Chinese medicine composition for hair growth and development comprises, by weight, 10-30 parts of sanguisorba officinalis, 10-30 parts of dried ginger, 10-30 parts of orientalis leaves, 10-20 parts of loquat leaves, 0-20 parts of ginkgo leaves, 0-20 parts of dandelion, and 10-20 parts of liquorice.

[0018] Preferably, the Chinese medicine composition comprises, by weight: 10-30 parts of Sanguisorba officinalis, 10-30 parts of dried ginger, 10-30 parts of Platycladus orientalis leaves, 10-20 parts of loquat leaves, 10-20 parts of Ginkgo biloba leaves, 10-20 parts of dandelion, and 10-20 parts of licorice.

[0019] More preferably, the Chinese medicine composition comprises, by weight: 20-30 parts of Sanguisorba officinalis, 20 parts of dried ginger, 20 parts of Platycladus orientalis leaves, 10-20 parts of loquat leaves, 10 parts of Ginkgo biloba leaves, 10 parts of dandelion, and 10 parts of liquorice.

[0020] The traditional Chinese medicine composition for hair growth and development of the present invention can be prepared by the following method: after crushing the traditional Chinese medicine, weighing the traditional Chinese medicine powder according to the mass fraction of the above-mentioned traditional Chinese medicine, adding 5-20 times the amount of 60-95% ethanol to soak overnight, heating and extracting twice at 75°C, combining the extracts, filtering, and conventionally concentrating to a thick paste to obtain the traditional Chinese medicine composition.

[0021] The present invention also provides a traditional Chinese medicine hydrogel for hair growth and development, and the preparation method of the hydrogel is as follows:

[0022] ① Take 2%-3% Carbomer 940 as the matrix, take 4 parts of the matrix, add 1.5 parts of glycerol to obtain A;

[0023] ② Weigh about 2 parts of the Chinese medicine composition, add 1.5 parts of a 60% ethanol solution, and stir to mix thoroughly to obtain B;

[0024] ③ Slowly add B to A while stirring and mix well to obtain C;

[0025] ④ Add 0.2 parts of azone and 0.5 parts of essence to C, stir and mix thoroughly to obtain D;

[0026] ⑤ Slowly add triethanolamine dropwise to D while stirring until its viscosity reaches 3.5±0.5 Pa·s, and encapsulate to obtain the traditional Chinese medicine hydrogel.

[0027] The hair growth and development Chinese medicine composition or Chinese medicine hydrogel of the present invention can be used to prepare common cosmetics, medicinal cosmetics or Chinese medicine external preparations for hair growth and development.

[0028] The various Chinese medicinal materials described in the present invention include corresponding raw medicinal materials or their decoction pieces.

[0029] From the perspective of improving the scalp environment and activating hair follicles, the present invention combines active ingredients such as flavonoids, polysaccharides, organic acids in Chinese herbal medicines to conduct preliminary screening of Chinese herbal medicine compounds. At the same time, a mouse hair loss model is established to study the hair growth effect of the combined compound. It is found that the combination of Sanguisorba officinalis, Platycladus orientalis, dried ginger, loquat leaves, Ginkgo biloba leaves, dandelion, and licorice has good hair growth promoting effect, and is innovative and practical.

[0030] The traditional Chinese medicine composition provided by the present invention has been shown by animal experiments to have little toxic and side effects and can be used for a long time.

[0031] The positive effects of this invention are: the preparation is easy to use, the raw medicinal materials are readily available, the preparation process is simple, and most importantly, the animal model used for screening is scientifically and rationally designed. The hair loss model requires a month of induction to more closely resemble human hair loss symptoms, and then the model is used to screen hair growth formulas. Sufficient animal experiments have demonstrated that the combination of medicinal materials is scientifically and rationally designed, and its hair growth-promoting effect is improved compared to the previously published patent. Compared to the chemical agent minoxidil, it is safe, has no toxic side effects, and can be used long-term. DETAILED DESCRIPTION

[0032] The present invention will be further described below with reference to animal experiments. The following embodiments are merely typical embodiments of the present invention, and the scope of protection of the present invention is not limited to the contents described.

[0033] Table 1 Prescription of Example 1-2

[0034] Group Sanguisorba officinalis dried ginger Platycladus orientalis leaves loquat leaves Ginkgo leaves dandelion Licorice Swertia japonica <![CDATA[1 * ]]> 20 0 0 0 20 20 0 20 2 20 20 0 0 20 20 20 0 3 20 20 20 20 10 10 10 0 4 30 20 20 10 10 10 10 0 5 10 30 10 10 20 20 10 0 6 20 10 30 10 10 20 10 0

[0035] *Experiment Group 1 is an investigation of the aforementioned Chinese patent CN201810728202.6

[0036] Example 1:

[0037] Preparation of Chinese herbal medicine extracts: Weigh the medicinal materials in proportions according to groups 1, 2, and 6 in Table 1, add 10 times the amount of 95% ethanol, soak for 12 hours, reflux at 75°C for 2 hours, extract twice, combine the extracts, filter, and concentrate under reduced pressure at 55°C to a paste, thereby obtaining combined extracts 1, 2, and 6, respectively.

[0038] Example 2:

[0039] Extraction of Chinese herbal medicine: Weigh the medicinal materials in proportion according to groups 3, 4, and 5 in the table, add 10 times the amount of 60% ethanol, soak for 12 hours, reflux at 75°C for 2 hours, extract twice, combine the extracts, filter, and concentrate under reduced pressure at 55°C to a paste, thereby obtaining combined extracts 3, 4, and 5, respectively.

[0040] Example 3

[0041] Preparation of topical hydrogel:

[0042] ① Take 2%-3% Carbomer 940 as the matrix, take 4 parts of the matrix, add 1.5 parts of glycerol to obtain A;

[0043] ② Weigh 2 parts of the extract of the traditional Chinese medicine composition prepared in Example 1 or 2, add 1.5 parts of 60% ethanol solution, and stir to mix thoroughly to obtain B;

[0044] ③ Slowly add B to A while stirring and mix well to obtain C;

[0045] ④ Add 0.2 parts of azone and 0.5 parts of essence to C, stir and mix thoroughly to obtain D;

[0046] ⑤ Slowly add triethanolamine dropwise to D while stirring until its viscosity reaches 3.5±0.5 Pa·s, and encapsulate to obtain the external hair growth and development hydrogel.

[0047] Preparation of blank hydrogel: The same as the above preparation method, in step ②, the Chinese medicine composition is replaced by a blank matrix.

[0048] Example 4 Hair Growth Effect Investigation Example

[0049] Experimental animals: 60 male C57 mice, weighing 18-20 g, SPF grade, provided by Shanghai Xipu-Bikai Animal Laboratory Co., Ltd., Animal Production License No.: SCXK (Shanghai) 2013-0016

[0050] Experimental methods:

[0051] Mouse hair loss model establishment:

[0052] 1) Hair removal of mice: After the mice were anesthetized by intraperitoneal injection of 4% chloral hydrate (0.1 ml / 10 g), 1.5*1.0 cm hair was shaved with a razor while they were under anesthesia. 2 To remove the hair on the neck, leaving only very short hair on the skin, apply 0.6 ml of 6% sodium sulfide evenly to the hair removal area on the neck of each mouse. After the sodium sulfide remains on the neck of the mouse for 150 seconds, quickly wash away the hair on the neck to expose the skin on the neck of the mouse.

[0053] 2) Imiquimod-induced hair loss model: Mice with light red skin on the nape were selected, and imiquimod ointment was applied to the hair loss area at 0.1 g / mouse daily on Mondays, Wednesdays, Fridays, and Sundays. The nape skin was cleaned with normal saline before imiquimod administration to remove residual imiquimod from the previous application. After four weeks, the model was successfully established when obvious keratinization and hairlessness appeared on the nape skin of the mice, and imiquimod application was stopped.

[0054] Grouping and administration: The mice with successful modeling were divided into 8 groups, with 6 mice in each group, namely blank control group, positive group and observation group (6 groups); the blank control group was smeared with blank gel, the positive group was smeared with 0.2 ml / mouse of minoxidil tincture, a commercially available chemical hair growth drug, and the remaining 6 groups were respectively treated with hydrogels prepared from Chinese medicine compositions 1-6; the dosage was 0.2 g / time, twice a day, at 10 am and 4 pm (timed administration to ensure the stability of the experiment).

[0055] Determination of the experimental endpoint: The experimental endpoint is when the hair of the mice in the positive group completely recovers to the normal length of the non-hair loss area (about 30 days).

[0056] Notes: A: After hair removal, observe the skin color. Only mice with red skin have hair in the resting phase, so choose mice with red skin. B: Administer the drug gently for 2-3 minutes to allow for full absorption of the drug. Return the mouse to its cage approximately 15 minutes after administration to prevent the bedding from drying out the drug.

[0057] Test indicators: A. Scoring of mouse hair growth: The skin and hair growth of mice in each group were observed every 4 days. Mice with the same initial nape color in different groups were compared and their skin and hair growth were scored. The scoring criteria are as follows:

[0058]

[0059] * Irregular hair growth is calculated based on the area ratio, e.g. 50% red, 50% grey, then 0.5 points will be recorded.

[0060] B. Recording of new hair length: At the end of the experiment, pluck the longest hair from the depilatory area, select the three longest hairs, measure their lengths using a vernier caliper, record the lengths, and calculate the mean.

[0061] C. Recording of new hair weight: At the end of the experiment, use scissors to cut the same area of ​​new hair along the hair root in the depilatory area, weigh it on an analytical balance, and record the weight.

[0062] Experimental results:

[0063] Comparison of hair length and weight of mice:

[0064] Table 2 Comparison of hair length and weight among groups (n=6, x±SD)

[0065]

[0066]

[0067] Note: When the hair thickness is the same, the value of hair weight / hair length is positively correlated with the number of hair roots per unit area (hair density);

[0068] Because patients with hair loss need to increase the number of hair roots per unit area rather than the length, increasing the hair weight / hair length value is more practical than simply increasing the hair length.

[0069] The results show that by comparing the hair and hair weight / hair length values ​​of each group of mice, the hair length and hair weight / hair length values ​​of the blank control group were lower than those of the positive drug minoxidil group, indicating that the model was successful. The Chinese medicine composition groups 2-6 of the present invention were all higher than the blank control group to varying degrees, proving that they have hair growth effects. The hair density of the composition group 2-6 was higher than that of the composition group 1 (i.e., the patent CN201810728202.6 group), indicating that the composition of the present invention has achieved better hair growth effects than the existing Chinese medicine composition. After adding ginkgo leaves and dandelions, the hair growth density of the mice increased further, with a more significant hair growth effect.

Claims

1. A Chinese medicine composition for hair growth and development, characterized in that: The invention is prepared from the following raw materials by weight: 10-30 parts of sanguisorba officinalis, 10-30 parts of dried ginger, 10-30 parts of oriental arborvitae leaves, 10-20 parts of loquat leaves, 10-20 parts of ginkgo leaves, 10-20 parts of dandelion, and 10-20 parts of liquorice; The Chinese medicine composition is prepared by the following method: crushing the Chinese medicinal materials, weighing the Chinese medicinal material powder according to the weight of the Chinese medicinal materials, adding 5-20 times the amount of 60-95% ethanol to soak overnight, heating and extracting twice at 75°C, combining the extracts, filtering, and conventionally concentrating to a thick paste to obtain the Chinese medicine composition.

2. The Chinese medicine composition according to claim 1, wherein The invention is prepared from the following raw materials by weight: 20-30 parts of sanguisorba officinalis, 20 parts of dried ginger, 20 parts of oriental arborvitae leaves, 10-20 parts of loquat leaves, 10 parts of ginkgo leaves, 10 parts of dandelions and 10 parts of liquorice.

3. A traditional Chinese medicine hydrogel for hair growth and development, characterized in that: The hydrogel is prepared by the following method: ① Take 2%-3% Carbomer 940 as the matrix, take 4 parts of the matrix, add 1.5 parts of glycerol to obtain A; ② Weigh 2 parts of the traditional Chinese medicine composition according to claim 1, add 1.5 parts of 60% ethanol solution, and stir to mix thoroughly to obtain B; ③ Slowly add B to A while stirring and mix well to obtain C; ④ Add 0.2 parts of azone and 0.5 parts of essence to C, stir and mix thoroughly to obtain D; ⑤ Slowly add triethanolamine dropwise to D while stirring until its viscosity reaches 3.5±0.5 Pa·s, and encapsulate to obtain the traditional Chinese medicine hydrogel.

4. Use of the Chinese medicine composition according to claim 1 or 2 or the Chinese medicine hydrogel according to claim 3 in the preparation of common cosmetics for hair growth and development or Chinese medicine external preparations.

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