Detection chip and detection system

Abstract

A detection chip and a detection system, the detection chip includes a sample loading opening and at least one detection branch structure. Each of the at least one detection branch structure includes a detection part; the detection part includes a detection groove and a reaction reagent, the detection groove is connected to the sample loading opening, the reaction reagent is contained in the detection groove, and the detection part is configured to allow optical detection of the reaction reagent located in the detection groove. The detection chip can help realize automated detection and can help realize a detection device that is easy to carry.

Description

[0001] This application claims priority to Chinese Patent Application No. 201911159478.8 filed on November 22, 2019, the full text of which is incorporated by reference as a part of this application. Technical Field

[0002] Embodiments of the present disclosure relate to a detection chip and a detection system. Background Art

[0003] Microfluidic chip technology integrates the basic operating units such as sample preparation, reaction, separation, and detection involved in the fields of biology, chemistry, and medicine onto a chip with micron-scale microchannels, automatically completing the entire process of reaction and analysis. The chip used in this process is called a microfluidic chip, also known as a chip laboratory (Lab-on-a-chip). Microfluidic chip technology has the advantages of small sample usage, fast analysis speed, easy to make portable instruments, and suitable for instant and on-site analysis. It has been widely used in many fields such as biology, chemistry, and medicine. Summary of the invention

[0004] At least one embodiment of the present disclosure provides a detection chip, which includes a sample loading opening and at least one detection branch structure; each of the at least one detection branch structure includes a detection part, the detection part includes a detection groove and a reaction reagent, wherein the detection groove is connected to the sample loading opening, the reaction reagent is accommodated in the detection groove, and the detection part is configured to allow optical detection of the reaction reagent located in the detection groove.

[0005] For example, in the detection chip provided by at least one embodiment of the present disclosure, each of the at least one detection branch structure also includes a guide groove, the guide groove having a first end and a second end, the first end of the guide groove being connected to the sample loading opening, and the second end of the guide groove being connected to the detection groove, so that the detection groove is connected to the sample loading opening through the guide groove.

[0006] For example, the detection chip provided by at least one embodiment of the present disclosure also includes: a first substrate having a first surface, wherein the sample loading opening is a through hole in the first substrate, and the guide groove and the detection groove are formed on the first surface of the first substrate; a second substrate stacked on the first surface of the first substrate and allowing the optical detection to be performed at a position corresponding to the detection groove.

[0007] For example, in the detection chip provided by at least one embodiment of the present disclosure, each of the at least one detection branch structure also includes a water-absorbing film, which is accommodated in the detection groove and is at least partially stacked with the reaction reagent in a direction perpendicular to the first substrate.

[0008] For example, in the detection chip provided in at least one embodiment of the present disclosure, the water absorption film is arranged on a side of the reaction reagent away from the second substrate.

[0009] For example, in the detection chip provided in at least one embodiment of the present disclosure, the liquid storage capacity of the water-absorbing membrane is 10 μL-50 μL.

[0010] For example, in the detection chip provided by at least one embodiment of the present disclosure, the detection part also includes a guide groove located around the detection groove, and the guide groove is connected to the detection groove; the height of at least part of the guide groove is smaller than the height of the detection groove.

[0011] For example, in the detection chip provided in at least one embodiment of the present disclosure, the guide groove includes a guide wall in a sloped shape, and one end of the guide wall is connected to the side surface of the detection groove.

[0012] For example, in the detection chip provided by at least one embodiment of the present disclosure, the longitudinal sections of the guide groove and the detection groove are overall stepped.

[0013] For example, in the detection chip provided by at least one embodiment of the present disclosure, the guide groove at least partially surrounds the detection groove.

[0014] For example, in the detection chip provided in at least one embodiment of the present disclosure, the height of the detection groove is 0.2mm-5mm, the difference between the maximum height of the guide groove and the height of the detection groove is 0.1mm-1mm, and the width of the guide groove is 0.1mm-1mm.

[0015] For example, in the detection chip provided in at least one embodiment of the present disclosure, the detection portion further includes a liquid storage through hole penetrating the first substrate and communicating with the detection groove.

[0016] For example, in the detection chip provided in at least one embodiment of the present disclosure, the liquid storage through hole is connected to the center of the detection groove.

[0017] For example, in the detection chip provided in at least one embodiment of the present disclosure, the diameter of the liquid storage through hole is 0.2 mm-5 mm, and the ratio of the depth of the liquid storage through hole to the height of the detection groove is 0.5:1-10:1.

[0018] For example, in the detection chip provided in at least one embodiment of the present disclosure, the height of the guide groove is 0.1 mm-1.5 mm, and the width of the guide groove is 0.1 mm-2 mm.

[0019] For example, in the detection chip provided by at least one embodiment of the present disclosure, the ratio of the height of the guide groove to the width of the guide groove is 1:1-10:1.

[0020] For example, in the detection chip provided in at least one embodiment of the present disclosure, the inner wall of the guide groove is hydrophilic.

[0021] For example, in the detection chip provided by at least one embodiment of the present disclosure, the at least one detection branch structure includes a plurality of detection branch structures, and the plurality of detection branch structures are evenly distributed along the periphery of the sample loading opening.

[0022] For example, in the detection chip provided in at least one embodiment of the present disclosure, the sample loading opening includes a first body and a first protrusion protruding from the first body toward the guide groove, and the first protrusion is connected to the guide groove.

[0023] For example, in the detection chip provided in at least one embodiment of the present disclosure, the diameter of the first body is 1 mm-10 mm.

[0024] For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction reagent includes a reaction membrane and / or a block reaction reagent.

[0025] For example, in the detection chip provided by at least one embodiment of the present disclosure, the reaction film is in a compressed state along the thickness direction.

[0026] For example, in the detection chip provided by at least one embodiment of the present disclosure, the ratio of the thickness of the reaction film in a relaxed state to the height of the detection groove is 1:1-1:0.5.

[0027] For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction membrane is circular, or the reaction membrane is polygonal, and one corner of the polygon is directly connected to the second end of the guide groove.

[0028] For example, in the detection chip provided by at least one embodiment of the present disclosure, the reaction membrane includes a membrane body and a second protrusion protruding from the membrane body toward the guide groove, and at least a portion of the second protrusion is located in the guide groove; the membrane body has the same shape as the detection groove, which is circular.

[0029] For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction membrane is diamond-shaped.

[0030] For example, in the detection chip provided in at least one embodiment of the present disclosure, the diameter of the detection groove is 3 mm-15 mm, and the diameter of the reaction membrane is equal to or smaller than the diameter of the detection groove.

[0031] For example, in the detection chip provided by at least one embodiment of the present disclosure, the second substrate has a detection through hole at a position corresponding to the detection groove.

[0032] For example, in the detection chip provided in at least one embodiment of the present disclosure, the second substrate is opaque; or, the detection chip further includes a light-shielding layer, which covers the surface of the second substrate away from and / or close to the first substrate and exposes the detection through hole.

[0033] For example, in the detection chip provided in at least one embodiment of the present disclosure, the diameter of the detection through hole is 2 mm-10 mm.

[0034] For example, in the detection chip provided by at least one embodiment of the present disclosure, the material of the first substrate includes one or more of polymethyl methacrylate, polystyrene and polycarbonate.

[0035] For example, in the detection chip provided by at least one embodiment of the present disclosure, the material of the second substrate includes one or more of polymethyl methacrylate, polystyrene, polycarbonate and polyethylene terephthalate.

[0036] For example, in the detection chip provided in at least one embodiment of the present disclosure, the first substrate and the second substrate are combined by bonding, welding, adhering or clamping.

[0037] For example, the detection chip provided by at least one embodiment of the present disclosure also includes a bonding layer; wherein the bonding layer is located between the first substrate and the second substrate and is used to combine the first substrate and the second substrate, and the bonding layer includes an opening corresponding to the detection groove.

[0038] For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction reagent includes a matrix material and a detection reagent distributed in the matrix material, and the matrix material includes glass fiber, cotton fiber, or a composite fiber of glass fiber and cotton fiber.

[0039] For example, the detection chip provided by at least one embodiment of the present disclosure also includes: a first substrate having a first surface, wherein the sample loading opening is a through hole in the first substrate, and the guide groove and the detection groove are formed on the first surface of the first substrate; a second substrate stacked on the first surface of the first substrate and having a detection through hole at a position corresponding to the detection groove to allow the optical detection to be performed through the detection through hole; the at least one detection branch structure includes a plurality of detection branch structures, and the plurality of detection branch structures are evenly distributed along the periphery of the sample loading opening.

[0040] For example, the detection chip provided by at least one embodiment of the present disclosure also includes: a first substrate, having a first surface and a second surface relative to each other, wherein the sample loading opening is a through hole in the first substrate, the detection groove is formed on the first surface of the first substrate, and the guide groove is formed on the second surface of the first substrate; a second substrate, stacked on the first surface of the first substrate and allowing the optical detection at a position corresponding to the detection groove; and a third substrate, stacked on the second surface of the first substrate and sealing the sample loading opening and the guide groove.

[0041] For example, the detection chip provided by at least one embodiment of the present disclosure also includes: a first substrate having a first surface, wherein the sample loading opening, the guide groove and the detection groove are formed on the first surface of the first substrate, and the sample loading opening is a non-through hole in the first substrate; a second substrate, stacked on the first surface of the first substrate and exposing the sample loading opening, and allowing the optical detection to be performed at a position corresponding to the detection groove.

[0042] For example, the detection chip provided by at least one embodiment of the present disclosure further includes an optical calibration branch structure, wherein the optical calibration branch structure includes an optical path detection area, and the optical path detection area is configured to perform optical calibration.

[0043] For example, the detection chip provided by at least one embodiment of the present disclosure further includes a sample loading protrusion: wherein the sample loading protrusion protrudes from the first surface of the first substrate in a direction away from the second substrate, and one end of the sample loading protrusion is connected to the sample loading opening.

[0044] For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample loading protrusion includes a conical cavity, the volume of the conical cavity is 50μL-200μL, and the height of the conical cavity protruding from the first substrate is 1mm-20mm; the conical cavity includes a first end and a second end opposite to each other, the first end is connected to the sample loading opening, the diameter of the first end is 0.5mm-5mm, and the diameter of the second end is 1mm-20mm.

[0045] For example, in the detection chip provided in at least one embodiment of the present disclosure, the first substrate includes a first recess, the second substrate includes a second recess corresponding to the first recess, and the first recess and the second recess are used to fix the detection chip.

[0046] At least one embodiment of the present disclosure further provides a detection system, comprising: a detection device and the detection chip described in any embodiment of the present disclosure; the detection device is configured to detect the reaction reagent in the detection groove through the detection part.

[0047] For example, in the detection system provided by at least one embodiment of the present disclosure, the detection device includes: a light source configured to emit light to the reaction reagent; and a photoelectric detection device configured to receive light emitted from the light source and reflected by the reaction reagent. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below. Obviously, the drawings in the following description only relate to some embodiments of the present disclosure, but are not intended to limit the present disclosure.

[0049] Figure 1A and Figure 1B They are respectively a front perspective view and a back perspective view of a detection chip provided by at least one embodiment of the present disclosure;

[0050] Figure 2A and Figure 2B They are Figure 1A and Figure 1B An exploded view of the detection chip shown in FIG.

[0051] Figure 3A and Figure 3B They are Figure 1A and Figure 1B A front perspective view and a back perspective view of the first substrate of the detection chip shown;

[0052] Figure 4 for Figure 1A and Figure 1B A schematic diagram of the planar structure of the first surface of the first substrate of the detection chip shown;

[0053] Figure 5 for Figure 1A and Figure 1B A schematic diagram of the planar structure of the second substrate of the detection chip shown;

[0054] Figure 6 for Figure 1A and Figure 1B A schematic diagram of the structure of the reaction membrane of the detection chip shown;

[0055] Fig. 7A and Figure 7B They are respectively a front perspective view and a back perspective view of another detection chip provided by at least one embodiment of the present disclosure;

[0056] Figure 8 for Fig. 7A and Figure 7B An exploded view of the detection chip shown in FIG.

[0057] Fig. 9A and Fig. 9B They are Fig. 7A and Figure 7B A front perspective view and a back perspective view of the first substrate of the detection chip shown;

[0058] Fig.10 for Fig. 7A and Figure 7B A schematic diagram of the planar structure of the first surface of the first substrate of the detection chip shown;

[0059] Fig.11 An exploded diagram of another detection chip provided by at least one embodiment of the present disclosure;

[0060] Fig.12 for Fig.11 A schematic structural diagram of a first substrate of a detection chip shown;

[0061] Fig.13A for Fig.12 A partial structural schematic diagram of a first substrate of a detection chip shown;

[0062] Fig. 13B for Fig.13A Schematic diagram of the cross-sectional structure along line A-A';

[0063] Fig. 13C for Fig.13A Schematic diagram of the cross-sectional structure along the BB' line;

[0064] Fig.14 An exploded diagram of another detection chip provided by at least one embodiment of the present disclosure;

[0065] Fig.15A and Fig. 15B An exploded diagram of another detection chip provided by at least one embodiment of the present disclosure;

[0066] Fig.16 An exploded diagram of another detection chip provided for at least one embodiment of the present disclosure; and

[0067] Fig.17 A schematic diagram of a detection system provided in accordance with at least one embodiment of the present disclosure. DETAILED DESCRIPTION

[0068] In order to make the purpose, technical solution and advantages of the embodiments of the present disclosure clearer, the technical solution of the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings of the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, not all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

[0069] Unless otherwise defined, the technical terms or scientific terms used in the present disclosure should be understood by people with ordinary skills in the field to which the present disclosure belongs. The "first", "second" and similar words used in the present disclosure do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word cover the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0070] In the process of designing the microfluidic chip, the inventor hopes to integrate as many analysis and detection functions as possible into the chip to reduce the chip's dependence on external operations, thereby achieving automation and integration. For example, the sampling component and the analysis and detection component of the microfluidic chip can be integrated together, and the automation of the detection process can be achieved through structural design, thereby reducing the technical requirements for operators, reducing human errors, and making the obtained detection data more accurate.

[0071] At least one embodiment of the present disclosure provides a detection chip, which includes a sample loading opening and at least one detection branch structure. Each of the at least one detection branch structure includes a detection part; the detection part includes a detection groove and a reaction reagent, the detection groove is connected to the sample loading opening, the reaction reagent is contained in the detection groove, and the detection part is configured to allow optical detection of the reaction reagent located in the detection groove.

[0072] The detection chip provided by at least one of the above embodiments of the present disclosure can integrate multiple basic structural units or components for sample detection into the same chip, and complete the entire process of sample analysis and detection through active control or capillary action, thereby realizing an automated and integrated detection process. Therefore, the use of the above detection chip can reduce possible human errors in the detection process, improve the accuracy of the detection data, and at the same time, the overall appearance of the detection chip can be designed to be thin or miniaturized, which is helpful to realize a portable detection system.

[0073] The detection chip and detection system provided by the present disclosure are described below through several specific embodiments.

[0074] In some embodiments of the present disclosure, each of the at least one detection branch structure also includes a guide groove having a first end and a second end, the first end of the guide groove being connected to the sample loading opening, and the second end of the guide groove being connected to the detection groove, so that the detection groove is connected to the sample loading opening through the guide groove.

[0075] Figure 1A and Figure 1B They are respectively a front perspective view and a back perspective view of a detection chip provided by at least one embodiment of the present disclosure, Figure 2A and Figure 2B They are Figure 1A and Figure 1B The exploded view of the detection chip shown in FIG. Figure 1A and Figure 2A is a top view of the detection chip, showing the structure viewed from the front of the detection chip; Figure 1B and Figure 2B This is a bottom view of the detection chip, showing the structure viewed from the back side of the detection chip.

[0076] For example, Figure 1A-Figure 2B As shown, the detection chip 10 includes a sample addition opening 110 and at least one detection branch structure 120, for example, multiple detection branch structures 120, and six detection branch structures 120 are shown in the figure as an example for introduction. The sample addition opening 110 is used to add a sample to be detected, such as a sample to be detected such as breast milk, body fluid, blood, etc. The multiple detection branch structures 120 are evenly distributed along the periphery of the sample addition opening 110, so that the multiple detection branch structures 120 can independently realize the detection function.

[0077] For example, each of the plurality of detection branch structures 120 includes a flow guide groove 130 and a detection portion 140. The flow guide groove 130 has a first end and a second end, and the first end is connected to the sample loading opening 110. The detection portion 140 includes a detection groove 141 and a reaction reagent 150, the detection groove 141 is connected to the second end of the flow guide groove 130, and the reaction reagent 150 is accommodated in the detection groove 141. The detection portion 140 is configured to allow optical detection of the reaction reagent 150 in the detection groove 141.

[0078] For example, in some embodiments, the reaction reagent 150 may be Figure 1A-Figure 2B The membrane-shaped structure shown in the figure is also a reaction membrane; or, in some embodiments, the reaction reagent 150 can be a block or powder reaction reagent, for example, a block or powder freeze-dried reagent, etc.; or, in some embodiments, for example, when the detection chip includes multiple detection branch structures, the reaction reagent contained in a part of the detection grooves can be a membrane-shaped reaction membrane, and the reaction reagent contained in another part of the detection grooves can be a reaction reagent different from a membrane-shaped reaction membrane, for example, a block or powder reaction reagent, and the embodiments of the present disclosure are not limited to this.

[0079] The following describes the detection chip provided by the embodiment of the present disclosure by taking the reaction reagent 150 as a reaction membrane (ie, reaction membrane 150) as an example. It should be noted that the embodiments of the present disclosure include but are not limited to the above.

[0080] For example, in some embodiments, the detection chip also includes an optical calibration branch structure, which includes an optical path detection area, and the optical path detection area is configured to perform optical calibration, thereby improving the accuracy and precision of the detection data obtained through optical detection.

[0081] For example, in the case where the detection chip includes a plurality of detection branch structures, the optical calibration branch structure and the plurality of detection branch structures are evenly distributed along the periphery of the sample loading opening. No reaction film is provided in the optical calibration branch structure. For example, the other structures of the optical calibration branch structure except the reaction film can be substantially the same as or similar to the detection branch structure, so that the optical calibration branch structure can be provided integrally with the detection branch structure, thereby simplifying the preparation process and preparation cost of the detection chip.

[0082] For example, Figure 1A-Figure 2B Taking the detection chip 10 shown in FIG. 1 as an example, the reaction film 150 may not be provided in a detection branch structure 120 so that it can be used as an optical calibration branch structure of the detection chip 10 for optical calibration. Figure 2B The detection branch structure 120 shown in the figure is not provided with, for example, the reaction film 150 (and the water absorption film 170 described below) and is used as an optical calibration branch structure of the detection chip 10 .

[0083] For example, the optical calibration branch structure includes a light path detection area, which may be a position corresponding to the detection groove 141. Thus, when the detection device is used to perform optical detection on the reaction film 150 in the detection chip 10, the optical calibration branch structure may be used to calibrate the light path emitted by the detection device, such as calibrating the irradiation angle of the light path, thereby improving the accuracy of the obtained detection data.

[0084] It should be noted that in some embodiments of the present disclosure, two or more optical calibration branch structures may be provided for optical calibration according to actual needs. Figure 1A-Figure 2B Taking the detection chip 10 shown as an example, the reaction membrane 150, for example, may not be set in the two detection branch structures 120 to serve as optical calibration branch structures of the detection chip 10. The two optical calibration branch structures may be symmetrical relative to the center of the sample loading opening 110, thereby improving the accuracy and precision of the optical calibration and further improving the accuracy of the obtained detection data.

[0085] It should be noted that in some other embodiments of the present disclosure, according to actual needs, the detection chip can also be calibrated in other ways. Accordingly, the calibration branch structure can also be other types of calibration structures other than optical calibration. For example, in other types of calibration structures, a reaction film can be set according to actual needs, or a reaction film can be not set (or other structures or components that may affect the calibration, such as the water-absorbing film 170 described below). The embodiments of the present disclosure are not limited to this.

[0086] For example, the reaction membrane 150 includes a detection reagent; the sample to be detected added from the sample addition opening 110 enters the detection groove 141 through the guide groove 130 and reacts with the detection reagent in the reaction membrane 150. At this time, the detection of the sample to be detected, such as the presence or absence of a certain component in the sample to be detected or the size of the content, can be achieved by, for example, optical detection to detect the reaction between the detection reagent and the sample to be detected, such as the color change on the reaction membrane 150.

[0087] Therefore, the detection chip 10 provided by at least one embodiment of the present disclosure can realize the integration of multiple functions such as mixing, analysis and detection, and complete the whole process of sample analysis and detection through active control and capillary action, thereby realizing an automated and integrated detection process, reducing possible human errors in the detection process, and improving the accuracy of the detection data. In addition, in some embodiments, the detection chip 10 can place reaction membranes 150 including different detection reagents in the detection grooves 141 of multiple detection branch structures 120, so that different detection reagents can be used to simultaneously detect multiple indicators in the same sample, shorten the detection cycle of the sample, and thus realize the timely detection of multiple indicators of the sample.

[0088] For example, in some examples, the sample to be detected can be a liquid, such as a breast milk sample. During the manufacturing process of the detection chip 10, the required detection reagent (such as a color developing reagent) can be pre-titrated on the initial reaction membrane 150, and the detection reagent is immersed in the reaction membrane 150 and then dried, and then the reaction membrane 150 including the detection reagent is placed in the detection groove of the detection chip 10, which is conducive to the storage and transportation of the detection chip 10. When the detection chip 10 is used for detection, the sample is added from the sample addition opening 110, so that the sample flows into the detection groove 141 through the guide groove 130 and mixes with the detection reagent on the reaction membrane 150 to react. Thus, by detecting the reaction results (for example, by identifying the change in color on the reaction membrane 150), it is possible to judge, for example, the presence or absence of the object to be detected in the sample and the size of the content, thereby realizing the detection of a certain indicator of the sample.

[0089] For example, in at least one of the above embodiments, when the detection chip 10 is used to detect a sample, the amount of sample injected may be 60 μL-80 μL. Thus, the amount of sample required when the detection chip 10 is used to detect a sample can be reduced, thereby reducing or avoiding sample waste.

[0090] For example, in some embodiments, Figure 1A-Figure 2B As shown, each detection branch structure 120 may also include a water-absorbing film 170, which is accommodated in the detection groove 141 and is at least partially stacked with the reaction film 150 in the thickness direction of the reaction film 150 (that is, in a direction perpendicular to the first substrate 101 described below), for example, the two are in direct contact.

[0091] For example, the liquid storage capacity of each water-absorbing membrane 170 can be 10 μL-50 μL, for example, 30 μL. The water-absorbing membrane 170 can accommodate a certain amount of sample. When the liquid storage capacity of the water-absorbing membrane 170 is exceeded, the water-absorbing membrane 170 will no longer absorb the sample, thereby playing a role in sample quantification and facilitating sample quantity control.

[0092] For example, the material of the water-absorbing film 170 may include glass fiber, cotton fiber, or composite fiber of glass fiber and cotton fiber.

[0093] It should be noted that in some other embodiments of the present disclosure, the detection chip may not use a water-absorbing membrane. In this case, the reaction membrane 150 can simultaneously play the role of sample quantification and reaction carrier.

[0094] It should be noted that in the embodiment of the present disclosure, the lengths, widths and depths of the multiple guide grooves 130 are the same as each other, thereby controlling the amount of samples flowing into different detection grooves 141 to remain relatively uniform, which is beneficial to controlling the amount of samples in the detection grooves 141. At the same time, the reaction time between the samples in different detection grooves 141 and the detection reagents on the reaction membrane 150 can also be kept relatively consistent, thereby improving the accuracy of the obtained detection data.

[0095] In some other embodiments of the present disclosure, for example, when multiple indicators in the same sample are detected simultaneously using different detection reagents, the multiple guide grooves 130 can have different sizes, such as different lengths, widths or depths, according to the different indicators of the samples to be detected. This can control the sample amount in different detection grooves 141 and the reaction time between the samples in different detection grooves 141 and the detection reagents on the reaction membrane 150, thereby obtaining multiple more accurate detection data at the same time according to actual needs.

[0096] It should be noted that, in the embodiments of the present disclosure, the specific length, width and depth of the flow guide groove 130 can be determined according to actual requirements such as sample quantity, sample characteristics, etc. For example, under the premise of making the sample flow from the sample loading opening 110 into the detection groove 141, the length of the flow guide groove 130 can be appropriately reduced to reduce or avoid waste of the sample during the flow process.

[0097] In some other embodiments of the present disclosure, the detection chip 10 may not be provided with the guide groove 130, for example, the detection groove 141 is directly connected to the sample loading opening 110 to achieve communication, thereby further reducing or avoiding the waste of the sample during the flow process. At this time, in some examples, the sample loading opening 110 may have a guide structure that is respectively connected to multiple detection grooves 141, so that the sample added to the sample loading opening 110 can flow evenly into the multiple detection grooves 141.

[0098] For example, in some embodiments, the sizes of the multiple detection grooves 141 can also be selected according to the different indicators of the samples to be detected, the amount of samples required, and the types of detection reagents. According to actual needs, the sizes and shapes of the multiple detection grooves 141 can be the same or different, and the embodiments of the present disclosure are not limited to this.

[0099] For example, in some embodiments, the detection chip 10 further includes a first substrate 101 and a second substrate 102. The first substrate 101 has a first surface and a second surface opposite to each other, the sample loading opening 110 is a through hole in the first substrate 101, and the flow guide groove 130 and the detection groove 141 are formed on the first surface of the first substrate 101. The second substrate 102 is stacked on the first surface of the first substrate 101 and allows optical detection at a position corresponding to the detection groove 141.

[0100] For example, Figure 3A and Figure 3B They are Figure 1A and Figure 1B The front perspective view and the back perspective view of the first substrate of the detection chip are shown. Figure 3A is a top view of the first substrate 101, showing the front structure of the first substrate. Figure 3B It is a bottom view of the first substrate 101 , showing the back structure of the first substrate. Figure 4 for Figure 1A and Figure 1B The schematic diagram of the planar structure of the first surface of the first substrate of the detection chip shown in FIG. Figure 5 for Figure 1A and Figure 1B The schematic diagram of the planar structure of the second substrate of the detection chip is shown.

[0101] For example, Figure 1A-Figure 5As shown, when the detection chip has the water absorption film 170 , the water absorption film 170 may be disposed on a side of the reaction film 150 away from the second substrate 102 , that is, disposed between the reaction film 150 and the detection groove 141 .

[0102] For example, the second substrate 102 has a detection through hole 160 at a position corresponding to the detection groove 141 , and the reaction film 150 in the detection groove 141 can be optically detected through the detection through hole 160 to obtain the detection index of the sample.

[0103] For example, the detection through hole 160 passes through the second substrate 102, and the diameter of the detection through hole 160 can be set to 2mm-10mm, further can be 0.5mm-4mm, for example, can be set to 3mm. In this way, optical detection can be achieved through the detection through hole 160, and the interference between the light rays directed to different detection through holes 160 can be reduced or avoided, thereby further improving the accuracy and precision of optical detection.

[0104] For example, all parts of the second substrate 102 except the detection through holes 160 can be set as opaque parts, thereby reducing or avoiding interference between light rays directed to different detection through holes 160, and reducing or avoiding light crosstalk between the detection through holes 160. For example, the opaque part of the second substrate 102 can be formed by dyeing or the like.

[0105] For example, a light shielding layer may also be provided on the side of the second substrate 102 away from the first substrate 101, and the light shielding layer covers the surface of the second substrate 102 away from the first substrate 101 except for the position with the detection through hole 160, that is, the light shielding layer covers the surface of the second substrate 102 away from the first substrate 101 and exposes the detection through hole 160, so that the interference between the light rays directed to different detection through holes 160 can be reduced or avoided, and the light crosstalk between the detection through holes 160 can be reduced or avoided. For example, the light shielding layer is made of an opaque material, and the light shielding layer can be provided on the surface of the second substrate 102 by, for example, pasting, printing, etc., and the embodiment of the present disclosure does not limit the specific method of forming the light shielding layer.

[0106] For example, the above-mentioned light shielding layer may also be provided on the side of the second substrate 102 close to the first substrate 101, that is, the light shielding layer covers the surface of the second substrate 102 close to the first substrate 101 and exposes the detection through-hole 160, so as to also reduce or avoid the interference between the light rays directed to different detection through-holes 160. Alternatively, in some embodiments of the present disclosure, a light shielding layer may be provided on both the side of the second substrate 102 away from the first substrate 101 and the side of the second substrate 102 close to the first substrate 101, and the two light shielding layers respectively cover the other parts of the surface of the second substrate 102 away from and close to the first substrate 101 except for the position with the detection through-hole 160, thereby better reducing or avoiding the interference between the light rays directed to different detection through-holes 160, and further reducing or avoiding the optical crosstalk between the detection through-holes 160.

[0107] For example, in some other embodiments of the present disclosure, the detection through hole may not be provided on the second substrate of the detection chip. In this case, the second substrate may be made of a transparent material that allows light to pass through, so that the reaction film in the detection groove can be directly optically detected through the second substrate.

[0108] For example, the second substrate may be made of a transparent material that allows light to pass through, and a detection window may be formed on the surface of the second substrate away from the first substrate by printing or pasting an opaque light-shielding layer to allow light to pass only through the detection window. For example, in some embodiments, the light-shielding layer may also be provided on the surface of the second substrate on the side close to the first substrate, or the surface of the second substrate on the side close to the first substrate and the surface on the side away from the first substrate may be provided with a light-shielding layer at the same time, and the embodiments of the present disclosure are not limited to this. The embodiments of the present disclosure are not limited to the specific material used for the second substrate, as long as the reaction film in the detection groove of the first substrate can be optically detected through the second substrate or the detection through hole opened on the second substrate.

[0109] It should be noted that, in the embodiment of the present disclosure, the number of the detection grooves 141 provided on the first substrate 101 and the number of the detection through holes 160 provided on the second substrate 102 and corresponding to the detection grooves 141 are only exemplary, and the embodiment of the present disclosure does not limit the specific number of the detection grooves 141 and the detection through holes 160. For example, the first substrate 101 may be provided with 1-20 detection grooves 141, and correspondingly, the second substrate 102 may be provided with 1-20 detection through holes 160.

[0110] For example, the material of the second substrate 102 may include one or more of polymethyl methacrylate, polystyrene, polycarbonate, and polyethylene terephthalate. For example, the material of the first substrate 101 may include one or more of polymethyl methacrylate, polystyrene, and polycarbonate, or may be other materials with high light transmittance. The embodiments of the present disclosure do not specifically limit the materials of the first substrate 101 and the second substrate 102.

[0111] For example, the first substrate 101 and the second substrate 102 can be combined by bonding (referring to the technology of bonding two pieces of material into one piece through van der Waals force, molecular force and atomic force, etc.), welding, bonding or clamping, thereby simplifying the processes such as processing and assembly of the detection chip 10 and reducing the preparation cost of the detection chip 10. For example, the first substrate 101 and the second substrate 102 can be combined by ultrasonic bonding, thermal compression bonding, laser welding, ultrasonic welding or silicone sealing. The embodiment of the present disclosure does not limit the specific bonding method between the first substrate 101 and the second substrate 102.

[0112] For example, the shape of the first substrate 101 may be circular, rectangular or other suitable shapes, and the shape of the second substrate 102 may also be circular, rectangular or other suitable shapes.

[0113] For example, in the case where the shape of the first substrate 101 is circular, the diameter of the first substrate 101 can be 3cm-15cm, and further can be 3cm-5cm. Since the detection chip 10 needs to cooperate with, for example, a detection device when in use, the first substrate 101 of the above size can reasonably reduce the space required for the detection chip 10 to occupy, so that the detection chip 10 can be designed to be thin, thereby facilitating the use of the detection chip 10 with the detection device, and reducing the transportation, packaging, storage, and other costs of the detection chip 10. For example, in the case where the shape of the second substrate 102 is circular, the diameter of the second substrate 102 can be 3cm-15cm, and further can be 3cm-5cm. Since the detection chip 10 needs to cooperate with, for example, a detection device when in use, the second substrate 102 of the above size can reasonably reduce the space required for the detection chip 10 to occupy, so that the detection chip 10 can be designed to be thin, thereby facilitating the use of the detection chip 10 with the detection device, and reducing the transportation, packaging, storage, and other costs of the detection chip 10.

[0114] For example, in the case where the shape of the first substrate 101 is rectangular, the diagonal of the first substrate 101 can be 3cm-15cm, and further can be 3cm-5cm. For example, the rectangular first substrate 101 can simplify the preparation process of the detection chip 10, reduce the processing technology and precision requirements of the detection chip 10, and thus reduce the preparation cost of the detection chip 10. In the case where the shape of the second substrate 102 is rectangular, the diagonal of the second substrate 102 can be 3cm-15cm, and further can be 3cm-5cm. For example, the rectangular second substrate 102 can simplify the preparation process of the detection chip 10, reduce the processing technology and precision requirements of the detection chip 10, and thus reduce the preparation cost of the detection chip 10.

[0115] For example, the shapes and sizes of the first substrate 101 and the second substrate 102 may be the same or similar to each other to facilitate the combination of the first substrate 101 and the second substrate 102 .

[0116] For example, the thickness of the first substrate 101 can be 0.5 mm-10 mm, such as 3 mm or 5 mm. For example, the thickness of the second substrate 102 can be 0.1 mm-5 mm, such as 0.5 mm or 2 mm. In this way, the space required for the detection chip 10 to be occupied can be reasonably reduced, so that the detection chip 10 can be designed to be thin, thereby reducing the costs of transportation, packaging, storage, etc. of the detection chip 10, and facilitating the use of the detection chip 10 in conjunction with the detection device. In addition, the above numerical range is also helpful for the processing and preparation of the detection chip 10, and is convenient for the user to grasp with one hand, fix or pick up using the device, etc.

[0117] For example, in one example, the diameter of the first substrate 101 and the second substrate 102 is 3.5 cm, the thickness of the first substrate 101 is 1.5 mm, and the thickness of the second substrate 102 is 1 mm. Thus, while the detection chip 10 is designed to be thin and convenient for users to grasp with one hand, fix or pick up with a device, the preparation process of the detection chip 10 can be simplified, and the processing technology and precision requirements of the detection chip 10 can be reduced, thereby facilitating the processing and preparation of the detection chip 10.

[0118] For example, Figure 1A-Figure 5 As shown, in some embodiments, the detection chip 10 may further include a sample loading protrusion 190. The sample loading protrusion 190 protrudes from the first surface of the first substrate 101 in a direction away from the second substrate 102, one end of the sample loading protrusion 190 is connected to the sample loading opening 110, and the other end may be connected to the atmosphere. The sample loading protrusion 190 may play the role of sample accommodation and flow diversion, so that the sample can quickly flow to the sample loading opening 110 through the sample loading protrusion 190, thereby facilitating the injection of the sample into the sample loading opening 110, increasing the sample volume of the detection chip 10, and facilitating, for example, the taking and placement of the detection chip 10.

[0119] For example, the sample loading protrusion 190 includes a conical cavity, and the sample loading opening 110 is connected to the atmosphere. For example, the cone angle of the conical cavity (for example, the angle between the center line of the cone and the side wall) can be 30°-75°, and the volume is 50μL-200μL. For example, the conical cavity of the sample loading protrusion 190 includes a first end and a second end opposite to each other, the first end is connected to the sample loading opening 110 and has a diameter of 0.5mm-5mm, and the diameter of the second end is 1mm-20mm. The height of the conical cavity of the sample loading protrusion 190 protruding from the first substrate 101 is 1mm-20mm. Thus, when the detection chip 10 is used in conjunction with, for example, a detection device, the sample loading protrusion 190 can occupy a reasonable space to match the size of the detection device, thereby facilitating the use of the detection chip 10 in conjunction with the detection device. In addition, the sample loading protrusion 190 determined by the above numerical range can better play the role of sample accommodation and flow guidance, for example, allowing the sample to flow to the sample loading opening 110 evenly and at a reasonable speed, thereby facilitating the injection of the sample into the sample loading opening 110 .

[0120] For example, in one example, the diameter of one end of the sample loading protrusion 190 connected to the sample loading opening 110 is 3 mm, the diameter of the other end is 10 mm, and the height of the sample loading protrusion 190 protruding from the first substrate 101 is 1.5 mm. In this way, the sample can be quickly flowed to the sample loading opening 110 through the sample loading protrusion 190, and it is also helpful for, for example, taking and placing the detection chip 10 and using it with, for example, a detection device. At the same time, the preparation process of the sample loading protrusion 190 can also be simplified, thereby reducing the processing technology and precision requirements.

[0121] For example, in some embodiments, Figure 4 As shown, the sample loading opening 110 includes a first body 111 and a first protrusion 112 protruding from the first body 111 toward the flow guide groove 130 . The first protrusion 112 is connected to the flow guide groove 130 to promote the sample to quickly flow into the flow guide groove 130 through the first protrusion 112 .

[0122] For example, the diameter of the first body 111 can be set to 1 mm-10 mm, such as 5 mm or 7 mm, etc. In this way, it can be ensured that the sample can flow into the guide groove 130 evenly and quickly, and the space required by the detection groove 141 can be ensured.

[0123] For example, the shape of the first body 111 can be circular, square or other suitable shapes, and the first protrusion 112 can be square, pointed or other shapes, and the embodiments of the present disclosure are not limited to this. For example, when the shape of the first body 111 is square, the diagonal length of the first body 111 can be set to 1mm-10mm, such as 5mm or 7mm. For example, a circular or square first body 111 can simplify the preparation process of the detection chip 10, reduce the processing technology and precision requirements of the detection chip 10, and thus reduce the preparation cost of the detection chip 10.

[0124] For example, the inner wall of the flow guide groove 130 can be set to be hydrophilic, for example, the contact angle of the liquid on the inner wall of the flow guide groove 130 is less than 90°, thereby facilitating the sample to quickly enter the flow guide groove 130, and allowing the sample to quickly flow into the detection groove 141 through the flow guide groove 130 to mix with the reaction membrane 150, thereby shortening the detection time of the sample and improving the accuracy of the obtained detection data. For example, in the process of preparing the detection chip, a hydrophilic treatment agent can be injected into the flow guide groove 130, so that the flow guide groove 130 is soaked in the hydrophilic treatment agent for 1 minute and then the hydrophilic treatment agent is discharged with air, so that the inner wall of the flow guide groove 130 can be set to be hydrophilic.

[0125] It should be noted that the contact angle of the liquid on the inner wall of the guide groove 130 refers to the angle formed by the tangent along the surface of the droplet and the inner wall surface of the guide groove 130. For example, when the contact angle of the liquid on the inner wall of the guide groove 130 is less than 90°, the inner wall of the guide groove 130 can be considered to be hydrophilic. The smaller the value of the contact angle of the liquid on the inner wall of the guide groove 130, the better the wettability of the inner wall of the guide groove 130. For example, when the contact angle of the liquid on the inner wall of the guide groove 130 is 0°, the inner wall material of the guide groove 130 is completely wetted. The embodiments of the present disclosure do not limit the specific value of the contact angle of the liquid on the inner wall of the guide groove 130, as long as the contact angle is less than 90° to make the inner wall of the guide groove 130 hydrophilic.

[0126] For example, in some embodiments, the height of the flow guide groove 130 can be set to 0.1 mm-1.5 mm, such as 0.5 mm; the width of the flow guide groove 130 can be set to 0.1 mm-2 mm, such as 0.5 mm. This helps control the amount of sample flowing into the detection groove 141 through the flow guide groove 130, reducing or avoiding waste of the sample during the flow process.

[0127] For example, in some embodiments, the ratio of the height of the flow guide groove 130 to the width of the flow guide groove 130 can be set to 1:1-10:1, such as 2:1. Therefore, in some embodiments, when the first substrate 101 and the second substrate 102 are combined by a bonding method, for example, the first surface of the first substrate 101 and the second substrate 102 are bonded by an adhesive, at this time, the adhesive can be made of a hydrophobic material. Since there is a hydrophobic adhesive between the first surface of the first substrate 101 and the bonding surface of the second substrate 102, by increasing the ratio of the height of the flow guide groove 130 to the width of the flow guide groove 130, the contact area between the sample and the hydrophobic adhesive on the bonding surface of the second substrate 102 when flowing through the flow guide groove 130 can be reduced, thereby achieving rapid flow of the sample in the flow guide groove 130.

[0128] For example, in one example, the height of the flow guide groove 130 is 1 mm, and the width of the flow guide groove 130 is 0.5 mm. This not only helps to achieve rapid flow of the sample in the flow guide groove 130, but also reduces or avoids waste of the sample during the flow process, and also simplifies the preparation process of the flow guide groove 130, reducing the processing technology and precision requirements.

[0129] It should be noted that, in some embodiments, the second end of the flow guide groove 130 (i.e., the end connected to the detection groove 141) is in contact with the reaction membrane 150, so as to help the sample to be immersed in the reaction membrane 150, so that the sample and the detection reagent in the reaction membrane 150 can fully react, for example, a part of the reaction membrane 150 can be located in the flow guide groove 130. In some embodiments, the reaction membrane 150 may not be in contact with the second end of the flow guide groove 130, and the embodiments of the present disclosure are not limited to this.

[0130] For example, in the embodiment of the present disclosure, structures of the detection chip 10 such as the sample loading protrusion 190 , the sample loading opening 110 , the guide groove 130 , the detection groove 141 , etc. can all be integrally formed by, for example, an injection molding process, thereby simplifying the preparation process of the detection chip 10 .

[0131] For example, in some embodiments, the reaction membrane 150 includes a matrix material and a detection reagent distributed in the matrix material. The matrix material may include glass fiber, cotton fiber, or a composite fiber of glass fiber and cotton fiber.

[0132] For example, in the detection chip of some embodiments, when the first substrate 101 and the second substrate 102 are combined, the reaction film 150 is in a compressed state compressed by the two substrates in the thickness direction. For example, the thickness of the reaction film 150 in the relaxed state can be equal to or slightly higher than the height of the detection groove 141, for example, the ratio of the thickness of the reaction film 150 in the relaxed state to the height of the detection groove 141 can be 1:1-1:0.5, and then after the first substrate 101 and the second substrate 102 are combined, since the reaction film 150 is compressed in the thickness direction, the surface of the side of the reaction film 150 facing the second substrate 102 and the first surface of the first substrate 101 can be located on the same horizontal plane, so that the reaction film 150 is in close contact with the second substrate 102, and the gap between the reaction film 150 and the first substrate 101 or the second substrate 102 can be avoided to cause the liquid storage phenomenon. As a result, the sample entering can be more fully immersed in the reaction film 150 and react more fully with the detection reagent in the reaction film 150, thereby improving the accuracy of the obtained detection data.

[0133] For example, in some embodiments of the present disclosure, the compression amount of the reaction film 150 can be set to 10%-40%, which helps to make the reaction film 150 in close contact with the second substrate 102, thereby reducing or avoiding the formation of gaps between the reaction film 150 and the first substrate 101 or the second substrate 102 to cause liquid accumulation.

[0134] For example, the shape of the reaction membrane 150 is usually the same as or similar to the shape of the detection groove 141 , so that the reaction membrane 150 can be accommodated in the detection groove 141 and the reaction membrane 150 and the sample can be mixed evenly.

[0135] For example, Figure 6 Shows Figure 1A and Figure 1B An exemplary structure of the reaction membrane of the detection chip is shown.

[0136] For example, Figure 4 and Figure 6 As shown, in some embodiments, the reaction membrane 150 includes a membrane body 151 and a second protrusion 152. The membrane body 151 has the same shape as the detection groove 141, and both are circular. The second protrusion 152 protrudes from the membrane body 151 toward the flow guide groove 130, and at least part of the second protrusion 152 is located in the flow guide groove 130, which can help introduce the sample from the flow guide groove 130 into the detection groove 141, and allow the sample to be fully immersed in the reaction membrane 150, so that the sample and the detection reagent in the reaction membrane 150 fully react, thereby improving the accuracy of the obtained detection data.

[0137] For example, the diameter of the detection groove 141 can be set to 3 mm-15 mm, and the diameter of the membrane body 151 of the reaction membrane 150 is equal to or smaller than the diameter of the detection groove 141. Thus, the reaction membrane 150 can be better accommodated in the detection groove 141, which helps to mix the reaction membrane 150 and the sample evenly.

[0138] For example, in some other embodiments of the present disclosure, the entire reaction membrane may also be set to the same shape as the detection groove, that is, the reaction membrane may only have a membrane main body but not a protruding part (such as the second protrusion 152), and the embodiments of the present disclosure are not limited to this.

[0139] For example, in some other embodiments of the present disclosure, the reaction membrane can also be set to a polygon, and one corner of the polygon is directly connected to the second end of the guide groove. Accordingly, the detection groove is a polygon with the same or similar shape as the reaction membrane.

[0140] For example, the reaction membrane may be arranged in a regular shape such as a triangle, a square, a rhombus, etc., or may be arranged in an irregular shape, etc., and the embodiments of the present disclosure are not limited to this.

[0141] For example, Fig. 7A and Figure 7B The front perspective view and the back perspective view of another detection chip provided by at least one embodiment of the present disclosure are shown respectively. Fig. 7A To test the top view of the chip, Figure 7B This is a bottom view of the detection chip. Figure 8 for Fig. 7A and Figure 7B The exploded view of the detection chip shown in FIG.

[0142] For example, Figure 7A-Figure 8 As shown, the detection chip 20 includes a sample loading opening 210 and a plurality of detection branch structures 220, and the plurality of detection branch structures 220 are evenly distributed along the periphery of the sample loading opening 210. Each of the plurality of detection branch structures 220 includes a flow guide groove 230 and a detection portion 240. The flow guide groove 230 has a first end and a second end, and the first end is connected to the sample loading opening 210. The detection portion 240 includes a detection groove 241 and a reaction reagent (e.g., a reaction membrane 250), the detection groove 241 is connected to the second end of the flow guide groove 230, the reaction membrane 250 is accommodated in the detection groove 241, and the detection portion 240 is configured to allow optical detection of the reaction membrane 250 located in the detection groove 241.

[0143] For example, Figure 7A-Figure 8 As shown, the reaction film 250 of the detection chip 20 is configured to be a polygon, such as a square or a rhombus, and accordingly, the shape of the detection groove 241 is also configured to be a square or a rhombus.

[0144] For example, the side length of the detection groove 241 can be set to 3 mm-15 mm, such as 5 mm or 10 mm, and the side length of the reaction membrane 250 is equal to or less than the side length of the detection groove 241. Thus, the reaction membrane 250 can be better accommodated in the detection groove 241, which helps to mix the reaction membrane 250 and the sample evenly.

[0145] For example, in the case where the shape of the detection groove 341 is set to a square, the diagonal of the square can be set to 1mm-20mm, such as 10mm or 15mm. For example, in the case where the shape of the detection groove 341 is set to a rhombus, the two diagonals of the rhombus can be set to 1mm-20mm respectively; for example, the length of one diagonal is 10mm, and the length of the other diagonal is 6mm. For example, the length ratio between the two diagonals of the rhombus can be set to 1:1 to 1:2. For example, the angle between two adjacent sides of the rhombus can be set to be less than or equal to 60°, so as to help the sample to be fully immersed in the reaction membrane 250 along the periphery of the reaction membrane 250, so that the sample can fully react with the detection reagent in the reaction membrane 250, thereby improving the accuracy of the obtained detection data.

[0146] For example, a corner of the reaction membrane 250 is directly connected to the second end of the guide groove 230 to introduce the sample from the guide groove 230 into the detection groove 241, and allow the sample to be fully immersed in the reaction membrane 250, so that the sample can fully react with the detection reagent in the reaction membrane 250, thereby improving the accuracy of the obtained detection data.

[0147] Fig. 9A and Fig. 9B They are Fig. 7A and Figure 7B The front perspective view and the back perspective view of the first substrate of the detection chip shown. For example, Fig. 9A is a top view of the first substrate 201, Fig. 9B It is a bottom view of the first substrate 201. Fig.10 for Fig. 7A and Figure 7B The schematic diagram of the planar structure of the first surface of the first substrate of the detection chip is shown.

[0148] For example, combined with Figure 7A to Figure 10 As shown, the detection chip 20 further includes a first substrate 201 and a second substrate 202. The first substrate 201 has a first surface and a second surface opposite to each other, the sample loading opening 210 is a through hole in the first substrate 201, and the guide groove 230 and the detection groove 241 are formed on the first surface of the first substrate 201. The second substrate 202 is stacked on the first surface of the first substrate 201 and allows optical detection at a position corresponding to the detection groove 241.

[0149] For example, the second substrate 202 has a detection through hole 260 at a position corresponding to the detection groove 241 , and the reaction film 250 in the detection groove 241 can be optically detected through the detection through hole 260 to obtain different detection indicators of the sample.

[0150] For example, in an embodiment of the present disclosure, the shape of the detection through hole 260 is circular, while in some other embodiments of the present disclosure, the shape of the detection through hole 260 can also be set to, for example, a square, a diamond or other shape that is the same as the shape of the detection groove 241, and the embodiments of the present disclosure are not limited to this.

[0151] For example, Fig.10 As shown, the sample loading opening 210 includes a first body 211 and a first protrusion 212 protruding from the first body 211 toward the flow guide groove 230 . The first protrusion 212 is connected to the flow guide groove 230 so that the sample flows into the flow guide groove 230 through the first protrusion 212 .

[0152] It should be noted that the materials, specific structural parameters, etc. of the first substrate 201 and the second substrate 202 can refer to the corresponding description of the first substrate 101 and the second substrate 102 of the detection chip 10 in the above embodiment, and will not be repeated here. The specific structural parameters, materials and functions of the detection chip 20 can refer to the corresponding description of the detection chip 10 in the above embodiment, and will not be repeated here.

[0153] For example, in some embodiments of the present disclosure, the detection portion of the detection chip may further include a flow guide groove located around the detection groove, for example, the flow guide groove may at least partially surround the detection groove. The flow guide groove is formed on the first surface of the first substrate and is connected to the detection groove, and the height of at least part of the flow guide groove is less than the height of the detection groove. Thus, the sample can be rapidly infiltrated along a portion of the peripheral area of ​​the reaction membrane through the flow guide groove, and a capillary force is generated between the flow guide groove and the reaction membrane to the sample to play a role in drainage, so that the sample is infiltrated from the periphery of the reaction membrane to the central area of ​​the reaction membrane, thereby accelerating the mixing speed between the sample and the reaction membrane, and further shortening the detection time. In addition, in the case where the sample is infiltrated from the periphery of the reaction membrane, due to the capillary action, the amount of sample infiltrated into the central area of ​​the reaction membrane will be increased, thereby making the detection result (such as color development) of the central area of ​​the reaction membrane more obvious, which is convenient for subsequent observation of the detection results, thereby improving the accuracy of the obtained detection data.

[0154] For example, in some embodiments of the present disclosure, the guide groove surrounds the detection groove, that is, surrounds all peripheral areas of the detection groove, so that the sample can be more quickly infiltrated along the periphery of the reaction membrane through the guide groove, and then infiltrated from the periphery of the reaction membrane to the center of the reaction membrane, thereby further accelerating the mixing speed between the sample and the reaction membrane, and more significantly shortening the required detection time. In addition, in the case where the sample is infiltrated from all sides of the reaction membrane at the same time, due to capillary action, the amount of sample infiltrated into the center of the reaction membrane increases, thereby making the detection result (such as color development) at the center of the reaction membrane the most obvious, which is convenient for the observation of subsequent detection results, thereby further improving the accuracy of the obtained detection data.

[0155] It should be noted that, in some embodiments of the present disclosure, the guide groove may also intersect or partially overlap with the detection groove in a direction perpendicular to the first surface of the first substrate, and the embodiments of the present disclosure are not limited to this.

[0156] For example, Fig.11 An exploded view, that is, a disassembled view, of another detection chip provided in at least one embodiment of the present disclosure. Fig.12 for Fig.11 The schematic diagram of the structure of the first substrate of the detection chip shown. For example, Fig.12 for Fig.11 The bottom view of the first substrate 301 of the detection chip 30 is shown. It should be noted that, in addition to the liquid storage through hole 380 and the guide groove 342, Fig.11 The detection chip 30 shown is Figure 7A-Figure 8 The structure and function of the detection chip 20 shown are substantially the same or similar, and will not be described in detail herein.

[0157] For example, combined with Fig.11 and Fig.12 As shown, the detection portion 340 of the detection chip 30 includes a reaction reagent (eg, a reaction membrane 350 ), a detection groove 341 , and guide grooves 342 and 343 surrounding the detection groove 341 .

[0158] Fig.13A for Fig.12 The schematic diagram of the partial structure of the first substrate of the detection chip shown in the figure, for example, the schematic diagram of the partial structure of the first substrate 301 of the detection chip 30 including the detection part 340, Fig. 13B for Fig.13A Schematic diagram of the cross-sectional structure along the A-A' line, Fig. 13C for Fig.13A It should be noted that for the sake of simplicity and clarity, Figure 12-13C The reaction film 350 of the detection section 340 is not shown.

[0159] For example, Figure 11-13CAs shown, the guide grooves 342 and 343 are formed on the first surface 3011 of the first substrate 301 and are connected to the detection groove 341. For example, one end of the guide groove 342 is connected to the guide groove 330, and the other end is connected to the guide groove 343. Therefore, when the sample flows into the detection part 340 through the guide groove 330, the sample will first flow into the guide grooves 342 and 343 and quickly infiltrate along the periphery of the reaction membrane 350 through the guide grooves 342 and 343, and then infiltrate from the periphery of the reaction membrane 350 to the center of the reaction membrane 350, thereby accelerating the mixing speed between the sample and the reaction membrane 350 and shortening the required detection time. In addition, due to the capillary action, the amount of sample infiltrated into the center of the reaction membrane 350 is increased, thereby making the detection result (such as color development) of the center of the reaction membrane 350 more obvious, which is convenient for the observation of subsequent detection results, thereby improving the accuracy of the obtained detection data.

[0160] For example, combined with Fig.13A and Fig. 13B As shown, the longitudinal sections of the guide groove 342 and the detection groove 341 are stepped as a whole, thereby forming a larger gap for the sample to flow between the guide groove 342 and the reaction membrane 350, thereby further improving the flow speed of the sample in the guide groove 342, so that the sample can quickly infiltrate along the four sides of the reaction membrane 350, and then infiltrate from the four sides of the reaction membrane 350 to the center of the reaction membrane 350, thereby improving the infiltration effect of the center of the reaction membrane 350, and making the detection result (such as color development) of the center of the reaction membrane 350 more obvious.

[0161] For example, combined with Fig.13A and Fig. 13B As shown, the height H2 of the guide groove 342 is less than the height H1 of the detection groove 341. For example, the height H1 of the detection groove 341 can be set to 0.2mm-5mm, such as 2mm or 3mm, so that when the first substrate 301 and the second substrate 302 are combined, the reaction film 350 is in a compressed state compressed by the two substrates along the thickness direction, so that the surface of the reaction film 350 facing the second substrate 302 and the first surface 3011 of the first substrate 301 can be located on the same horizontal plane, so that the reaction film 350 and the second substrate 302 are in close contact, and a gap between the reaction film 350 and the first substrate 301 or the second substrate 302 can be avoided to cause liquid storage. The difference between the height H2 of the guide groove 342 and the height H1 of the detection groove 341 is 0.1mm-1mm, such as 0.5mm or 0.8mm, so that the sample can flow quickly in the detection groove 341, so that the sample can be more quickly infiltrated along the periphery of the reaction film 350.

[0162] For example, the width D1 of the flow guiding groove 342 can be set to 0.1 mm-1 mm, such as 0.5 mm or 0.7 mm, etc. Thus, a sufficient and appropriate gap for the sample to flow is formed between the flow guiding groove 342 and the reaction membrane 350, thereby facilitating the sample to infiltrate from the periphery of the reaction membrane 350 to the center of the reaction membrane 350, improving the infiltration effect of the center of the reaction membrane 350, and making the detection result (such as color development) of the center of the reaction membrane 350 more obvious.

[0163] For example, in the case where the shape of the detection groove 341 is set to a square, the diagonal of the square can be set to 1mm-20mm, such as 10mm or 15mm. For example, in the case where the shape of the detection groove 341 is set to a rhombus, the two diagonals of the rhombus can be set to 1mm-20mm respectively; for example, the length of one diagonal is 10mm, and the length of the other diagonal is 6mm. For example, the length ratio between the two diagonals of the rhombus can be set to 1:1~1:2. For example, the angle between two adjacent sides of the rhombus can be set to be less than or equal to 60°, which helps the sample to infiltrate the center of the reaction membrane 350 along the four sides of the reaction membrane 250, so that the sample can be fully immersed in the reaction membrane 250, thereby improving the accuracy of the obtained detection data.

[0164] For example, in some other embodiments of the present disclosure, the guide groove 342 may also be designed to be stepped including a multi-layer step structure, which is not limited in the embodiments of the present disclosure.

[0165] For example, combined with Fig.13A and Fig. 13C As shown, the flow guide groove 343 is set to a sloped structure. For example, the flow guide groove 343 includes a sloped flow guide wall 344, which is inclined relative to the first surface 3011 of the first substrate 301, and the first end 3441 of the flow guide wall 344 is connected to the first surface 3011 of the first substrate 301, and the second end 3442 of the flow guide wall 344 is connected to the side surface of the detection groove 341. Since the gap for sample flow formed between the flow guide groove 343 and the reaction membrane 350 is smaller than that of the flow guide groove 342, the flow speed of the sample in the flow guide groove 343 is slightly smaller than the flow speed of the sample in the flow guide groove 342. Therefore, after the sample flows into the guide groove 343 through the guide groove 342, the sample can be reduced or prevented from remaining excessively in the guide groove 343, thereby further increasing the speed at which the sample infiltrates from the periphery of the reaction membrane 350 to the center of the reaction membrane 350, thereby increasing the amount of sample infiltrating into the center of the reaction membrane 350, and thereby making the detection result (such as color development) at the center of the reaction membrane 350 more obvious, which is more conducive to the observation of subsequent detection results.

[0166] It should be noted that in some other embodiments of the present disclosure, the guide groove 343 can also be set to other suitable structures, as long as the space for sample flow formed between the guide groove 343 and the reaction membrane 350 is less than or equal to the space for sample flow formed between the guide groove 342 and the reaction membrane 350. The embodiments of the present disclosure do not limit this.

[0167] For example, combined with Fig.12 , Fig.13A and Fig. 13C As shown, the second end 3442 of the guide wall 344 is not parallel to the first surface 3011 of the first substrate 301, for example, the second end 3442 of the guide wall 344 is inclined relative to the first surface 3011 of the first substrate 301 and extends toward the first surface 3011 of the first substrate 301 along the flow direction of the sample (for example, along the direction away from the sample loading opening 310). In other words, the surface area of ​​the guide wall 344 (that is, the contact area between the guide wall 344 and the sample) gradually decreases along the flow direction of the sample, for example, gradually decreases along the direction away from the sample loading opening 310, so that the space for the sample to flow formed between the guide groove 343 and the reaction membrane 350 gradually decreases along the flow direction of the sample. Thus, the speed of the sample infiltrating from the periphery of the reaction membrane 350 to the center of the reaction membrane 350 is further increased, so that the amount of the sample infiltrating to the center of the reaction membrane 350 is further increased to a certain extent, thereby making the detection result (for example, color development) of the center of the reaction membrane 350 more obvious, which is more conducive to the observation of subsequent detection results, thereby improving the accuracy of the obtained detection data.

[0168] It should be noted that in some other embodiments of the present disclosure, the guide wall 344 can also be set to other suitable contour shapes, as long as the contact area between the guide wall 344 and the sample is gradually reduced along the flow direction of the sample. The embodiments of the present disclosure do not limit this.

[0169] For example, the maximum height H4 of the flow guiding groove 343 is smaller than the height H1 of the detection groove 341, that is, the distance between the second end of the flow guiding wall 344 connected to the side surface of the detection groove 341 and the first surface 3011 of the first substrate 301 is smaller than the height H1 of the detection groove 341. Thus, a gap for the sample to flow can be formed between the flow guiding groove 343 and the reaction film 350, so that the sample can quickly infiltrate along the periphery of the reaction film 350, and then infiltrate from the periphery of the reaction film 350 to the center of the reaction film 350, thereby improving the infiltration effect of the center of the reaction film 350, and making the detection result (such as color development) of the center of the reaction film 350 more obvious.

[0170] For example, the difference between the distance between the second end of the guide wall 344 and the first surface 3011 of the first substrate 301 and the height of the detection groove 341 (i.e., the difference between H4 and H1) can be set to 0.1 mm-1 mm. Thus, an appropriate gap for sample flow can be formed between the guide groove 343 and the reaction film 350, thereby improving the wetting effect of the center of the reaction film 350.

[0171] For example, the width D2 of the guide groove 343 can be set to 0.1 mm-1 mm, such as 0.5 mm or 0.7 mm, so that an appropriate gap for sample flow is formed between the guide groove 343 and the reaction membrane 350, thereby improving the wetting effect of the center of the reaction membrane 350.

[0172] It should be noted that in Figure 11-13C In the above-mentioned embodiment shown, the width D1 of the guide groove 342 is the same as the width D2 of the guide groove 343, and the height H2 of the guide groove 342 is the same as the maximum height H4 of the guide groove 343; while in some other embodiments of the present disclosure, the width D1 of the guide groove 342 and the width D2 of the guide groove 343 may also be different from each other, and the height H2 of the guide groove 342 and the maximum height H4 of the guide groove 343 may also be different from each other, and the embodiments of the present disclosure are not limited to this.

[0173] It should be noted that in Figure 11-13C In the above-mentioned embodiment shown, the guide grooves surrounding the detection groove 341 include a guide groove 342 and a guide groove 343 having different structures from each other, while in some other embodiments of the present disclosure, the guide grooves surrounding the detection groove 341 may also adopt the same structure, for example, all are set to be the guide groove 342, or all are set to be the guide groove 343, and the embodiments of the present disclosure are not limited to this.

[0174] It should be noted that in Figure 11-13C In the above embodiment shown, the structure of the guide groove is described by taking the example of opening a guide groove around the diamond-shaped detection groove. In other embodiments of the present disclosure, when the detection groove is a triangle, a circle or other regular or irregular shape, a guide groove can also be opened around the corresponding detection groove. For example, Figure 1A-Figure 2B The detection groove 141 of the detection chip 10 shown in the figure is provided with a guide groove around it, but the embodiment of the present disclosure is not limited to this.

[0175] For example, in an embodiment of the present disclosure, the guide grooves 342 and 343 surround the detection groove 341 and the contour shape of the guide grooves 342 and 343 is the same as the contour shape of the detection groove 341. In some other embodiments of the present disclosure, the contour shape of the guide grooves 342 and 343 may also be different from the contour shape of the detection groove 341, and the embodiments of the present disclosure are not limited to this.

[0176] For example, in some other embodiments of the present disclosure, the flow guide groove may also partially surround the detection groove. For example, taking the diamond-shaped detection groove as an example, the flow guide groove may be provided only on the two sides of the detection groove adjacent to the flow guide groove to achieve rapid mixing between the sample and the reaction membrane. At the same time, the detection result (e.g., color development) in a certain area or a certain range near the center of the reaction membrane may be made more obvious, thereby facilitating the subsequent observation of the detection result and improving the accuracy of the obtained detection data.

[0177] For example, Fig.12 and Fig.13A As shown, the guide groove 330 extends into the detection groove 341, and the second end of the guide groove 330 communicating with the detection groove 341 is located in the diamond-shaped detection groove 341, so that the sample can more quickly and fully infiltrate the reaction membrane 350, shortening the required detection time.

[0178] For example, the length of the second end of the flow guide groove 330 extending into the detection groove 341 is less than or equal to 1.5 mm, such as 0.5 mm or 1 mm, etc. This can help introduce the sample from the flow guide groove 330 into the detection groove 341, so that the sample can be fully immersed in the reaction membrane 350, so that the sample and the detection reagent in the reaction membrane 350 can fully react, thereby improving the accuracy of the obtained detection data.

[0179] For example, in some embodiments of the present disclosure, the reaction film 350 has a thickness in the thickness direction (i.e., along the Fig.13A or Fig. 13B 341). For example, the thickness of the reaction film 350 in a relaxed state may be equal to or slightly higher than the height H1 of the detection groove 341. For example, the ratio of the thickness of the reaction film 350 in a relaxed state to the height H1 of the detection groove 341 may be 1:1-1:0.5. After the first substrate 301 and the second substrate 302 are combined, the reaction film 350 is compressed in the thickness direction, and the reaction film 350 is in close contact with the second substrate 302, thereby avoiding the formation of a gap between the reaction film 350 and the first substrate 301 or the second substrate 302 to cause liquid storage.

[0180] For example, Fig.11 and Fig.12As shown, the detection portion 340 may further include a liquid storage hole 380 that passes through the first substrate 301 and is connected to the detection groove 341. In the event that an excessive amount of sample is injected through the sample loading opening 310, the excess sample may be stored in the liquid storage hole 380, thereby avoiding or reducing leakage caused by excessive sample injection. The liquid storage hole 380 may also promote the sample to infiltrate from the periphery to the center of the reaction membrane 350 through the guide grooves 342 and 343, so that the detection result (e.g., color development) at the center of the reaction membrane 350 is more obvious.

[0181] For example, the diameter of the liquid storage hole 380 can be set to 0.2mm-5mm, such as 2mm or 3mm, etc., which is conducive to controlling the sample amount, avoiding or reducing leakage caused by excessive sample injection, and promoting the sample to infiltrate from the periphery to the center of the reaction membrane 350. It can also reduce the processing technology and precision requirements of the liquid storage hole 380, thereby simplifying the preparation process.

[0182] For example, Fig. 13B and Fig. 13C As shown, the ratio of the depth H3 of the liquid storage hole 380 to the height H1 of the detection groove 341 can be set to 0.5:1-10:1, such as 3:1, etc., so as to play a role in sample quantification and help control the sample amount. At the same time, the liquid storage hole 380 can also store more samples to prevent sample overflow.

[0183] For example, the liquid storage hole 380 is connected to the center of the detection groove 341, that is, the liquid storage hole 380 is opened at the central position of the bottom surface of the detection groove 341, connecting the center of the detection groove 341 with the external environment such as the atmosphere, thereby better promoting the sample to infiltrate from the periphery to the center of the reaction membrane 350.

[0184] For example, in the manufacturing process of the detection chip 30, in order to facilitate the assembly of the detection chip 30, during assembly, negative pressure can be added to the liquid storage through hole 380 to fix the reaction membrane 350 in the detection groove 341. In this way, the sample can react more fully with the detection reagent in the reaction membrane 350, further improving the accuracy of the obtained detection data.

[0185] For example, in one example of the present disclosure, the shape of the detection groove 341 is a rhombus and the two diagonals of the rhombus are 8 mm and 5.8 mm respectively, the height H1 of the detection groove 341 is 0.5 mm, the difference between the height H2 of the guide groove 342 and the height H1 of the detection groove 341 is 0.3 mm, the difference between the maximum height H4 of the guide groove 343 and the height H1 of the detection groove 341 is 0.3 mm, the width D1 of the guide groove 342 is 0.3 mm, the width D2 of the guide groove 343 is 0.3 mm, the diameter of the liquid storage through hole 380 is 2.5 mm, and the depth H3 of the liquid storage through hole 380 is 1 mm. Furthermore, it is helpful to control the amount of sample in the detection groove 341, avoid or reduce the leakage caused by excessive sample injection, and at the same time promote the sample to infiltrate from the periphery to the center of the reaction membrane 350, so that the amount of sample infiltrated into the center of the reaction membrane 350 is increased, thereby making the detection result (such as color development) in the center of the reaction membrane 350 more obvious, which is more conducive to the observation of subsequent detection results.

[0186] It should be noted that in some other embodiments of the present disclosure, the liquid storage through hole can also be provided at other positions on the bottom surface of the detection groove; or, according to actual needs, multiple liquid storage through holes can also be provided in each detection part. The embodiments of the present disclosure do not limit the location and number of the liquid storage through holes.

[0187] Fig.14 An exploded view, that is, a disassembled view, of another detection chip provided in at least one embodiment of the present disclosure. Fig.14 FIG. 7 is a top view of the detection chip, showing the structure viewed from the front of the detection chip. It should be noted that, except for the adhesive layer 704 and the sample loading protrusion 790, Fig.14 The detection chip 70 shown is Fig.11 The structure and function of the detection chip 30 shown are substantially the same or similar, and will not be described in detail herein.

[0188] For example, Fig.14 The detection chip 70 is shown in a state where the first substrate 701 and the second substrate 702 are combined via the adhesive layer 704 .

[0189] For example, Fig.14 As shown, the adhesive layer 704 is stacked between the first substrate 701 and the second substrate 702 , that is, stacked on the first surface of the first substrate 701 .

[0190] For example, the adhesive layer 704 may be made of a hydrophobic adhesive material, such as a double-sided tape.

[0191] For example, Fig.14As shown, the adhesive layer 704 includes an opening 7041 corresponding to the detection groove and the guide groove (not shown) of the first substrate 701, thereby avoiding obstruction to the flow of the sample due to the uneven surface of the adhesive layer 704 and its own viscosity, and also avoiding adhesion between the adhesive layer 704 and the reaction membrane 750, thereby facilitating the flow of the sample in the detection groove and the guide groove, allowing the sample to quickly and fully infiltrate the reaction membrane 750, thereby improving the accuracy of the acquired detection results.

[0192] For example, in some embodiments of the present disclosure, the shape and size of the opening 7041 may be the same as the shape and size of the cross section of the detection groove, so as to reduce or avoid the obstruction of the adhesive layer 704 to the flow of the sample, or reduce or avoid the adhesion between the adhesive layer 704 and the reaction membrane 750. In some embodiments of the present disclosure, the shape of the opening 7041 may be the same as the shape of the cross section of the detection groove, and the size of the opening 7041 may be slightly smaller than the size of the cross section of the detection groove, so as to slightly increase the contact area between the adhesive layer 704 and the first substrate 701 and the second substrate 702, so as to improve the adhesion between the first substrate 701 and the second substrate 702, thereby further reducing or avoiding the leakage of the sample and improving the sealing effect of the detection chip 70.

[0193] For example, the tip portion (eg, the top corner portion) of the opening 7041 may be set to a right angle, or may be set to a rounded corner with a certain curvature, such as a right angle or an arc. Fig.14 Taking the diamond-shaped opening 7041 in the figure as an example, the four corners of the diamond can be set to right angles or to rounded corners with a certain curvature, and the embodiments of the present disclosure are not limited to this.

[0194] For example, in some examples, Fig.14 Taking the diamond opening 7041 in the example, the two groups of diagonals of the diamond can be divided into a first group and a second group, and the first group of diagonals is set to a right angle, and the second group of diagonals is set to be in an arc shape. For example, since the shape of the diamond opening 7041 is substantially the same as the contour shape of the detection groove of the first substrate 701, by making the first group of diagonals in an arc shape, the size of the diamond opening 7041 can be slightly smaller than the size of the cross section of the detection groove, thereby increasing the contact area between the bonding layer 704 and the first substrate 701 and the second substrate 702 to a certain extent, and improving the bonding effect between the first substrate 701 and the second substrate 702; and by setting the second group of diagonals to a right angle, the obstruction of the bonding layer 704 to the flow of the sample can be reduced or avoided to a certain extent, and the bonding between the bonding layer 704 and the reaction membrane 750 can also be reduced or avoided.

[0195] Therefore, by setting the above-mentioned first group of diagonals and second group of diagonals, it is possible to reduce or avoid the problem that the adhesive layer 704 hinders the flow of the sample due to its own viscosity and the adhesion between the adhesive layer 704 and the reaction membrane 750, and it is also possible to better achieve the combination between the first substrate 701 and the second substrate 702, reduce or avoid sample leakage, and improve the sealing effect of the detection chip 70.

[0196] It should be noted that, in some other examples, all the top angles of the opening 7041 may be designed to be right angles, or may be designed to be arc-shaped or have other suitable contours, and the embodiments of the present disclosure are not limited to this.

[0197] It should be noted that in Fig.14 In the illustrated embodiment, the shape and size of the opening 7041 are substantially the same as the contour shape and size of the detection groove and the guide groove of the first substrate 701, while in some other embodiments of the present disclosure, the size of the opening of the adhesive layer may be slightly larger or smaller than the size of the detection groove or the guide groove, or may be set to other suitable shapes or sizes, as long as the obstruction of the adhesive layer to the sample infiltration reaction membrane can be avoided or reduced and the sealing effect of the detection chip can be ensured, and the embodiments of the present disclosure are not limited to this.

[0198] For example, Fig.14 As shown, compared to Fig.11 The detection chip 30 shown, the detection chip 70 adopts a sample loading protrusion 790 with a petal-shaped cross-section, thereby increasing the contact area between the sample loading protrusion 790 and the first substrate 701, facilitating a stable connection between the sample loading protrusion 790 and the first substrate 701, and being more suitable for users to hold with one hand, fix or pick up using a device, etc.

[0199] For example, Fig.14 As shown, the first substrate 701 includes a first notch 7011, and the second substrate 702 includes a second notch 7021 corresponding to the first notch 7011. In a direction perpendicular to the surface of the first substrate 701 or the second substrate 702, the first notch 7011 and the second notch 7021 overlap each other. The first notch 7011 and the second notch 7021 form a limiting portion (or a limiting block), so that the detection chip 70 can be fixed on, for example, a detection device through the limiting block formed by the first notch 7011 and the second notch 7021, thereby facilitating the detection of the reaction film 750 in the detection chip 70 and improving the accuracy of the acquired detection data. In addition, the positions of the first notch 7011 and the second notch 7021 can also be used to mark and locate multiple detection branch structures in the detection chip 70, thereby facilitating, for example, observation and recording of detection data.

[0200] It should be noted that the sizes of the first recess 7011 and the second recess 7021 can be determined according to the structure of, for example, a detection device to which they are to be fixed, and the embodiments of the present disclosure are not limited to this, as long as the detection chip 70 can be fixed on the detection device through the first recess 7011 and the second recess 7021.

[0201] For example, in a direction perpendicular to the surface of the first substrate 701 or the second substrate 702 , the first recess 7011 and the second recess 7021 may be disposed between adjacent detection grooves, or at other suitable positions, which is not limited in the embodiments of the present disclosure.

[0202] For example, when the detection chip 70 includes an adhesive layer 704 or other structural layers or functional layers, the structural layer or functional layer also has a notch structure at positions corresponding to the first notch 7011 and the second notch 7021, so that the detection chip 70 as a whole forms a limit block.

[0203] For example, in some embodiments of the present disclosure, the sample loading opening, flow guide groove, detection groove and other structures of the detection chip can also be arranged in other ways, as long as the arrangement of the sample loading opening, flow guide groove, detection groove and other structures can be realized and the corresponding functions can be realized. The above embodiments are introduced based on the situation that the sample loading opening passes through the first substrate and the flow guide groove, detection groove and other structures are arranged on the first surface of the first substrate of the detection chip, but this does not constitute a limitation of the present disclosure.

[0204] Below Fig.11 On the basis of the detection chip 30 shown, other feasible arrangement positions of the guide groove and the detection groove are exemplarily described.

[0205] For example, Fig.15A and Fig. 15B The following is an exploded view of another detection chip provided in at least one embodiment of the present disclosure, that is, a decomposition view. For example, Fig.15A To test the top view of the chip, Fig. 15B This is a bottom view of the test chip. It should be noted that: Fig.15A and Fig. 15B The structures and functions of the specific components in the detection chip 40 shown may refer to the corresponding descriptions of the detection chip 10 , the detection chip 20 or the detection chip 30 in the above embodiments, and will not be repeated here.

[0206] For example, Fig.15A and Fig. 15B As shown, the detection chip 40 includes a first substrate 401 , a second substrate 402 and a third substrate 403 .

[0207] For example, the first substrate 401 has a first surface (shown as an upper surface) and a second surface (shown as a lower surface) opposite to each other, the flow guide groove 430 is formed on the second surface of the first substrate 401, and the detection groove 441 is formed on the first surface of the first substrate 401. The second substrate 402 is stacked on the first surface of the first substrate 401 and has a detection through hole 460 at a position corresponding to the detection groove 441 to allow optical detection. The third substrate 403 is stacked on the second surface of the first substrate 401 to seal the flow guide groove 430 and the sample loading opening 410 so that the sample can flow from the sample loading opening 410 into the flow guide groove 430.

[0208] For example, the sample loading opening 410 is a through hole penetrating the first substrate 401 , and the second substrate 402 has a through hole exposing the sample loading opening 410 , so that the sample can be injected into the sample loading opening 410 , and the sample loading protrusion 490 can extend from the second substrate 402 , so as to facilitate, for example, taking and placing the detection chip 40 .

[0209] For example, Fig.16 The following is an exploded view of another detection chip provided in at least one embodiment of the present disclosure, that is, a decomposition view. For example, Fig.16 This is a top view of the detection chip. It should be noted that: Fig.16 The structures and functions of the specific components in the detection chip 50 shown may refer to the corresponding descriptions of the detection chip 10 , the detection chip 20 or the detection chip 30 in the above embodiments, and will not be repeated here.

[0210] For example, Fig.16 As shown, the detection chip 50 includes a first substrate 501 and a second substrate 502. The first substrate 501 has an opposite first surface (shown as an upper surface in the figure) and a second surface (shown as a lower surface in the figure), a guide groove 530 and a detection groove 541 are formed on the first surface of the first substrate 501, and the second substrate 502 is stacked on the first surface of the first substrate 501 and has a detection through hole 560 at a position corresponding to the detection groove 541 to allow optical detection.

[0211] For example, the sample loading opening 510 is an opening in the first substrate 501, and the opening is, for example, a non-through hole that does not penetrate the first substrate 501. In this case, the height (or depth) of the sample loading opening 510 is less than the thickness of the first substrate 501. The second substrate 502 has a through hole exposing the sample loading opening 510 and a sample loading protrusion 590, so that the sample can be injected into the sample loading opening 510 through the sample loading protrusion 590, and it is convenient for the detection chip 50 to be taken and placed.

[0212] At least one embodiment of the present disclosure further provides a detection system, which includes: a detection device and a detection chip provided by any embodiment of the present disclosure, such as the detection chip 10, the detection chip 20, the detection chip 30, the detection chip 40 or the detection chip 50 in the above embodiments, and the detection device is configured to detect the reaction membrane in the detection groove through the detection part of the detection chip.

[0213] For example, taking the detection chip 10 in the above embodiment as an example, Fig.17 A schematic diagram of a detection system provided in accordance with at least one embodiment of the present disclosure.

[0214] For example, Fig.17 As shown, the detection system 60 includes a detection chip 10 and a detection device 610 . The detection device 610 is configured to detect the reaction reagent, such as the reaction membrane 150 , in the detection groove 141 through the detection portion 140 of the detection chip 10 .

[0215] For example, the detection device 610 includes a light source 611 and a photodetection device 612. The light source 611 is configured to emit light toward the reaction film 150, and the photodetection device 612 is configured to receive light emitted from the light source 611 and reflected by the reaction film 150.

[0216] For example, the photoelectric detection device 612 can compare the intensity of the light reflected by the reaction film 150 with the intensity of the light emitted by the light source 611, so as to judge the presence and concentration of the object to be detected in the sample according to the value of the absorbance of the detected reaction film 150, so as to detect the sample index. For example, taking the detection reagent pre-titrated on the reaction film 150 as a color developing reagent, when obtaining the test result, the darker the color on the reaction film 150, the higher the content of the object to be detected in the sample to be detected, and the larger the value of the absorbance of the reaction film 150 detected by the photoelectric detection device 612.

[0217] For example, in some embodiments, the photoelectric detection device 612 can be a photodiode, which can convert the received light signal into an electrical signal, and then judge the intensity of the received light according to the change of electrical parameters in the electrical signal (such as the change of current, etc.), thereby determining the value of the absorbance of the reaction film 150.

[0218] The specific description and technical effects of the detection system provided by the embodiments of the present disclosure can refer to the corresponding contents in the detection chip provided by the embodiments of the present disclosure, for example, the corresponding contents of the detection chip 10, the detection chip 20, the detection chip 30, the detection chip 40 or the detection chip 50 in the above-mentioned embodiments can be referred to, and will not be repeated here.

[0219] There are a few points to note:

[0220] (1) The drawings of the embodiments of the present disclosure only relate to the structures related to the embodiments of the present disclosure, and other structures may refer to the general design.

[0221] (2) For the sake of clarity, in the drawings used to describe the embodiments of the present disclosure, the thickness of the layers or regions is exaggerated or reduced, that is, these drawings are not drawn according to the actual scale. It is understood that when an element such as a layer, a film, a region or a first substrate is referred to as being "on" or "under" another element, the element may be "directly" "on" or "under" the other element or there may be intermediate elements.

[0222] (3) In the absence of conflict, the embodiments of the present disclosure and the features therein may be combined with each other to obtain new embodiments.

[0223] The above are only specific implementations of the present disclosure, but the protection scope of the present disclosure is not limited thereto, and the protection scope of the present disclosure shall be based on the protection scope of the claims.

Claims

1. A detection chip, comprising: Add sample opening, At least one detection branch structure, wherein each of the at least one detection branch structure comprises: The detection part includes a detection groove and a reaction reagent, The detection groove is connected to the sample addition opening, and the reaction reagent is contained in the detection groove. The detection portion is configured to allow optical detection of the reaction reagent located in the detection recess, Wherein, each of the at least one detection branch structure further includes a guide groove, the guide groove having a first end and a second end, the first end of the guide groove being communicated with the sample loading opening, and the second end of the guide groove being communicated with the detection groove, so that the detection groove is communicated with the sample loading opening through the guide groove, The detection chip also includes: a first substrate having a first surface, wherein the sample loading opening is a through hole in the first substrate, and the guide groove and the detection groove are formed on the first surface of the first substrate; and a second substrate stacked on the first surface of the first substrate and allowing the optical detection to be performed at a position corresponding to the detection groove.

2. The detection chip according to claim 1, wherein: Each of the at least one detection branch structure further includes a water absorbing film, The water absorption film is accommodated in the detection groove, and at least partially overlaps with the reaction reagent in a direction perpendicular to the first substrate.

3. The detection chip according to claim 2, wherein: The water absorption film is arranged on a side of the reaction reagent away from the second substrate.

4. The detection chip according to claim 2 or 3, wherein: The liquid storage capacity of the water-absorbing membrane is 10 μL-50 μL.

5. The detection chip according to any one of claims 1 to 3, wherein: The detection portion further comprises a flow guiding groove located around the detection groove, and the flow guiding groove is communicated with the detection groove; The height of at least part of the guide grooves is smaller than the height of the detection grooves.

6. The detection chip according to claim 5, wherein: The guide groove comprises a guide wall in a slope shape. One end of the guide wall is connected to the side surface of the detection groove.

7. The detection chip according to claim 5, wherein: The longitudinal sections of the guide groove and the detection groove are stepped as a whole.

8. The detection chip according to claim 5, wherein: The guide groove at least partially surrounds the detection groove.

9. The detection chip according to claim 5, wherein: The height of the detection groove is 0.2mm-5mm, the difference between the maximum height of the guide groove and the height of the detection groove is 0.1mm-1mm, and the width of the guide groove is 0.1mm-1mm.

10. The detection chip according to any one of claims 1 to 3, wherein: The detection portion further includes a liquid storage through hole penetrating through the first substrate and communicating with the detection groove.

11. The detection chip according to claim 10, wherein: The liquid storage through hole is connected to the center of the detection groove.

12. The detection chip according to claim 10, wherein: The diameter of the liquid storage through hole is 0.2 mm-5 mm, and the ratio of the depth of the liquid storage through hole to the height of the detection groove is 0.5:1-10:

1.

13. The detection chip according to any one of claims 1 to 3, wherein: The height of the guide groove is 0.1mm-1.5mm, and the width of the guide groove is 0.1mm-2mm.

14. The detection chip according to any one of claims 1 to 3, wherein: The ratio of the height of the guide groove to the width of the guide groove is 1:1-10:

1.

15. The detection chip according to any one of claims 1 to 3, wherein: The inner wall of the guide groove is hydrophilic.

16. The detection chip according to any one of claims 1 to 3, wherein: The at least one detection branch structure includes a plurality of detection branch structures, and the plurality of detection branch structures are evenly distributed along the periphery of the sample loading opening.

17. The detection chip according to any one of claims 1 to 3, wherein: The sample addition opening includes a first body and a first protrusion protruding from the first body toward the guide groove, and the first protrusion is communicated with the guide groove.

18. The detection chip according to claim 17, wherein: The diameter of the first body is 1 mm-10 mm.

19. The detection chip according to any one of claims 1 to 3, wherein: The reaction reagent includes a reaction film and / or a block reaction reagent.

20. The detection chip according to claim 19, wherein: The reaction film is in a compressed state along the thickness direction.

21. The detection chip according to claim 20, wherein: The ratio of the thickness of the reaction film in a relaxed state to the height of the detection groove is 1:1-1:0.

5.

22. The detection chip according to claim 19, wherein: The reaction membrane is circular, or The reaction membrane is a polygon, and one corner of the polygon is directly connected to the second end of the guide groove.

23. The detection chip according to claim 19, wherein: The reaction membrane comprises a membrane body and a second protrusion protruding from the membrane body toward the guide groove, wherein at least a portion of the second protrusion is located in the guide groove; The membrane body has the same shape as the detection groove, which is circular.

24. The detection chip according to claim 19, wherein: The reaction membrane is rhombus-shaped.

25. The detection chip according to claim 22, wherein: The diameter of the detection groove is 3 mm-15 mm, and the diameter of the reaction membrane is equal to or smaller than the diameter of the detection groove.

26. The detection chip according to any one of claims 1 to 3, wherein: The second substrate has a detection through hole at a position corresponding to the detection groove.

27. The detection chip according to claim 26, wherein: The second substrate is opaque; or, The detection chip further includes a light shielding layer, which covers the surface of the second substrate away from and / or close to the first substrate and exposes the detection through hole.

28. The detection chip according to claim 26, wherein: The diameter of the detection through hole is 2mm-10mm.

29. The detection chip according to any one of claims 1 to 3, wherein: The material of the first substrate includes one or more of polymethyl methacrylate, polystyrene and polycarbonate.

30. The detection chip according to any one of claims 1 to 3, wherein: The material of the second substrate includes one or more of polymethyl methacrylate, polystyrene, polycarbonate and polyethylene terephthalate.

31. The detection chip according to any one of claims 1 to 3, wherein: The first substrate and the second substrate are combined by bonding, welding, adhering or clamping.

32. The detection chip according to claim 31, further comprising a bonding layer; in, The bonding layer is located between the first substrate and the second substrate and is used to combine the first substrate and the second substrate. The adhesive layer includes an opening corresponding to the detection groove.

33. The detection chip according to any one of claims 1 to 3, wherein: The reaction reagent includes a matrix material and a detection reagent distributed in the matrix material. The matrix material includes glass fiber, cotton fiber or composite fiber of glass fiber and cotton fiber.

34. The detection chip according to claim 1, wherein: The at least one detection branch structure includes a plurality of detection branch structures, and the plurality of detection branch structures are evenly distributed along the periphery of the sample loading opening.

35. The detection chip according to any one of claims 1 to 3, further comprising an optical calibration branch structure, in, The optical calibration branch structure includes an optical path detection area, and the optical path detection area is configured to perform optical calibration.

36. The detection chip according to any one of claims 1 to 3, further comprising a sample loading protrusion: in, The sample loading protrusion protrudes from the first surface of the first substrate in a direction away from the second substrate, One end of the sample adding protrusion is connected to the sample adding opening.

37. The detection chip according to claim 36, wherein: The sample adding protrusion comprises a conical cavity, The volume of the conical cavity is 50 μL-200 μL, and the height of the conical cavity protruding from the first substrate is 1 mm-20 mm; The conical cavity comprises a first end and a second end opposite to each other, the first end is connected to the sample addition opening, the diameter of the first end is 0.5 mm-5 mm, and the diameter of the second end is 1 mm-20 mm.

38. The detection chip according to any one of claims 1 to 3, wherein: The first substrate includes a first notch, and the second substrate includes a second notch corresponding to the first notch, The first recess and the second recess are used to fix the detection chip.

39. A detection system comprising: The detection chip according to any one of claims 1 to 38, The detection device is configured to detect the reaction reagent in the detection groove through the detection part.

40. The detection system according to claim 39, wherein: The detection device comprises: a light source configured to emit light toward the reaction reagent, The photoelectric detection device is configured to receive light emitted from the light source and reflected by the reaction reagent.

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