A layered imaging system, method and device

Abstract

An embodiment of the present invention provides a layered imaging system, method and device. The system includes: a microscope objective lens, a support assembly, a driving device, an imaging lens group and an imager; the microscope objective lens, the imaging lens group and the imager are coaxially mounted on the support assembly; the driving device drives the imaging lens group to move along the axis direction of the imaging lens group, so that the imaging lens group converges the received reflected light on the imager to sequentially generate spectral images of different heights of a sample to be imaged, perform layered imaging on the sample without moving the sample, and the imaging process is not restricted by the external environment, thereby improving the accuracy of the three-dimensional image.

Description

Technical Field

[0001] The present invention relates to the field of imaging technologies, and particularly to a layered imaging system, method and device. Background Art

[0002] In a micro fluorescence / Raman spectrometer system, it is not only necessary to image the surface of a sample, but also necessary to perform layered imaging on the interior of the sample, and finally form a three-dimensional fluorescence / Raman image consistent with the sample. In related technologies, during the imaging process, a stage needs to carry the sample and move in the Z-axis direction using a standard Z-axis displacement stage. However, if the sample to be measured needs to be placed in special environments such as vacuum, low temperature, high pressure, and high temperature, the stage cannot ensure high-precision movement, resulting in the final three-dimensional image being inconsistent with the sample; in another related technology, the position of the microscope objective is adjusted to adjust the position of the focus, but the accuracy of the overall movement of the microscope objective and the structures above it cannot be ensured, resulting in a poor accuracy of the final three-dimensional image. Summary of the Invention

[0003] An object of the present invention is to provide a layered imaging system that performs layered imaging on a sample without moving the sample, and the imaging process is not restricted by the external environment, thereby improving the accuracy of the three-dimensional image. Another object of the present invention is to provide a layered imaging method. Still another object of the present invention is to provide a layered imaging device. Yet another object of the present invention is to provide a computer-readable medium. Yet another object of the present invention is to provide a computer device.

[0004] To achieve the above objects, on the one hand, the present invention discloses a layered imaging system, including:

[0005] A microscope objective, a support assembly, a driving device, an imaging lens group, and an imager;

[0006] The microscope objective, the imaging lens group, and the imager are coaxially installed on the support assembly;

[0007] The driving device drives the imaging lens group to move along the axis direction of the imaging lens group, so that the imaging lens group converges the received reflected light on the imager to sequentially generate spectral images of different heights of the sample to be imaged.

[0008] Preferably, the support assembly includes: a microscope bracket;

[0009] The microscope objective is installed on the microscope bracket.

[0010] Preferably, the support assembly further includes: an adapter plate, a first fixing plate, a second fixing plate, and a third fixing plate;

[0011] The adapter board is installed on the top surface of the microscope bracket, the first fixing plate is installed on the adapter board, and the second fixing plate and the third fixing plate are both installed on the first fixing plate;

[0012] The imager is installed on the third fixing plate;

[0013] The adapter board is provided with a light passing hole for the reflected light of the sample to be imaged transmitted by the microscope objective to pass through;

[0014] The driving device is installed on the second fixing plate.

[0015] Preferably, the system further includes: a fixing device;

[0016] The imaging lens group is connected to the first fixing plate through the fixing device;

[0017] The fixing device includes a guide rail, a slider and an adapter block. Among them, the guide rail is fixed on the first fixing plate, the adapter block is fixedly connected to the imaging lens group, and the slider is fixedly connected to the adapter block and is arranged in the guide rail and can move along the guide rail.

[0018] Preferably, the driving device includes a motor and a lead screw, and the lead screw is respectively connected to the motor and the fixing device;

[0019] The motor drives the lead screw to rotate so that the lead screw drives the adapter block to move along the guide rail.

[0020] Preferably, the system further includes: a stray light removal system;

[0021] The stray light removal system is installed on the adapter board, and the stray light removal system is used to filter the stray light in the reflected light of the sample to be imaged transmitted by the microscope objective.

[0022] Preferably, the stray light removal system further includes: a first lens group, a pinhole and a second lens group;

[0023] The first lens group converges the reflected light of the sample to be imaged to the pinhole and transmits it to the second lens group through the pinhole;

[0024] The second lens group converts the received reflected light into parallel light and transmits the parallel light to the imaging lens group.

[0025] Preferably, the imager includes a detection surface; the imaging lens group includes at least one imaging lens;

[0026] The imaging lens is used to sequentially converge the reflected light received at different positions to the detection surface and generate a spectral image corresponding to different heights of the sample to be imaged on the detection surface.

[0027] Preferably, the system further includes: a stage;

[0028] The stage is arranged below the microscope objective and is installed on the support assembly to carry the sample to be imaged.

[0029] Preferably, the system further includes: a controller;

[0030] The controller is configured to generate a three-dimensional image of the sample to be imaged based on spectral images of different heights of the sample to be imaged by specifying an imaging algorithm.

[0031] The present invention also discloses a hierarchical imaging method, including:

[0032] Controlling a driving device to drive an imaging lens group to move along the axis direction of the imaging lens group;

[0033] Controlling an imager to receive the reflected light converged by the imaging lens group onto the imager, so that the imager sequentially generates spectral images of different heights of the sample to be imaged.

[0034] Preferably, controlling the driving device to drive the imaging lens group to move along the axis direction of the imaging lens group includes:

[0035] If the emitted light is divergent light, controlling the driving device to drive the imaging lens group to move downward along the axis direction of the imaging lens group;

[0036] If the emitted light is convergent light, controlling the driving device to drive the imaging lens group to move upward along the axis direction of the imaging lens group.

[0037] Preferably, before controlling the imager to receive the reflected light converged by the imaging lens group onto the imager, the method further includes:

[0038] Controlling a stray light removal system to filter stray light in the reflected light of the sample to be imaged transmitted by a microscope objective lens, and transmitting the filtered light after removing stray light to the imaging lens group.

[0039] Preferably, the stray light removal system further includes: a first lens group, a pinhole, and a second lens group;

[0040] The method further includes:

[0041] Converging the reflected light of the sample to be imaged to the pinhole through the first lens group, and transmitting it to the second lens group through the pinhole;

[0042] Converting the received reflected light into parallel light through the second lens group, and transmitting the parallel light to the imaging lens group.

[0043] Preferably, after controlling the imager to receive the reflected light converged by the imaging lens group onto the imager, so that the imager sequentially generates spectral images of different heights of the sample to be imaged, it further includes:

[0044] Generating a three-dimensional image of the sample to be imaged based on spectral images of different heights of the sample to be imaged by specifying an imaging algorithm.

[0045] The present invention also discloses a layered imaging device, including:

[0046] A first control unit, configured to control a driving device to drive an imaging lens group to move along the axis direction of the imaging lens group;

[0047] A second control unit, configured to control an imager to receive the reflected light converged by the imaging lens group on the imager, so that the imager sequentially generates spectral images of different heights of a sample to be imaged.

[0048] The present invention also discloses a computer-readable medium, on which a computer program is stored, and when the program is executed by a processor, the above-mentioned method is implemented.

[0049] The present invention also discloses a computer device, including a memory and a processor, where the memory is used to store information including program instructions, and the processor is used to control the execution of the program instructions, and when the processor executes the program, the above-mentioned method is implemented.

[0050] A layered imaging system provided by the present invention includes: a microscope objective lens, a support assembly, a driving device, an imaging lens group, and an imager; the microscope objective lens, the imaging lens group, and the imager are coaxially installed on the support assembly; the driving device drives the imaging lens group to move along the axis direction of the imaging lens group, so that the imaging lens group converges the received reflected light on the imager to sequentially generate spectral images of different heights of a sample to be imaged, performs layered imaging on the sample without moving the sample, and is not restricted by the external environment during the imaging process, thereby improving the accuracy of the three-dimensional image. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention, and for those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0052] Figure 1a It is a schematic structural diagram of a layered imaging system provided by an embodiment of the present invention;

[0053] Figure 1b It is a schematic structural diagram of a pinhole confocal system provided by an embodiment of the present invention;

[0054] Figure 2 It is a schematic optical path diagram of a layered imaging system provided by an embodiment of the present invention;

[0055] Figure 3 It is a schematic optical path diagram of another layered imaging system provided by an embodiment of the present invention;

[0056] Figure 4 Optical path schematic diagram of another layer imaging system provided by an embodiment of the present invention;

[0057] Figure 5 Flowchart of a layer imaging method provided by an embodiment of the present invention;

[0058] Figure 6 Flowchart of another layer imaging method provided by an embodiment of the present invention;

[0059] Figure 7 Optical path schematic diagram of another layer imaging system provided by an embodiment of the present invention;

[0060] Figure 8 Structural schematic diagram of a layer imaging device provided by an embodiment of the present invention;

[0061] Figure 9 Structural schematic diagram of a computer device provided by an embodiment of the present invention. Detailed implementation manners

[0062] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0063] To facilitate the understanding of the technical solutions provided in this application, the relevant content of the technical solutions in this application will be described first. In a micro-fluorescence / Raman spectrometer system, it is necessary to perform overall layer imaging on the surface and inside of a sample to form a three-dimensional fluorescence / Raman image consistent with the sample. During the imaging process, the stage carries the sample and moves along the Z-axis direction. The accuracy and stability of the stage's movement along the Z-axis direction and the resolution of the microscope objective play a decisive role in the accuracy of the final imaging. When the sample must be in a special environment, such as: vacuum, low temperature, high pressure, or high temperature and other special environments, it is difficult to control the high-precision movement of the stage, resulting in a poor accuracy of the finally formed three-dimensional image.

[0064] The present invention provides a layer imaging system that does not require moving the microscope objective and the sample, and realizes layer imaging of the sample by adjusting the imaging lens group, and can ensure the accuracy of the finally formed three-dimensional image.

[0065] Figure 1a Structural schematic diagram of a layer imaging system provided by an embodiment of the present invention, as Figure 1aAs shown, the system includes a microscopic objective lens 110, a support assembly 120, a driving device 130, an imaging lens group 140, and an imager 150.

[0066] The microscopic objective lens 110, the imaging lens group 140, and the imager 150 are coaxially mounted on the support assembly 120.

[0067] The driving device 130 drives the imaging lens group 140 to move along the axis direction of the imaging lens group 140, so that the imaging lens group 140 converges the received reflected light on the imager 150 to generate spectral images of different heights of the sample to be imaged in sequence.

[0068] In the embodiment of the present invention, the support assembly 120 includes a microscope bracket 121. As Figure 1a shown, the microscopic objective lens 110 is mounted on the microscope bracket 121, and the microscope bracket 121 is used to carry the microscopic objective lens 110.

[0069] In the embodiment of the present invention, the support assembly 120 further includes an adapter plate 122, a first fixing plate 123, a second fixing plate 124, and a third fixing plate 125. As Figure 1a shown, the adapter plate 122 is mounted on the top surface of the microscope bracket 121, the first fixing plate 123 is mounted on the adapter plate 122, and both the second fixing plate 124 and the third fixing plate 125 are mounted on the first fixing plate 123.

[0070] The imager 150 is mounted on the third fixing plate 125.

[0071] The adapter plate 122 is provided with a light passing hole for the reflected light of the sample to be imaged transmitted by the microscopic objective lens 110 to pass through.

[0072] The driving device 130 is mounted on the second fixing plate 124.

[0073] In the embodiment of the present invention, the system further includes: a fixing device. As Figure 1a shown, the imaging lens group 140 is connected to the first fixing plate 123 through the fixing device.

[0074] The fixing device includes a guide rail 161, a slider 162, and an adapter block 163. Among them, the guide rail 161 is fixed on the first fixing plate 123, the adapter block 162 is fixedly connected to the imaging lens group 140, the slider 162 is fixedly connected to the adapter block 163 and the slider 162 is arranged in the guide rail 161 and can move along the guide rail 161.

[0075] In the embodiment of the present invention, the driving device 130 includes a motor 131 and a lead screw 132. As Figure 1a shown, the lead screw 132 is respectively connected to the motor 131 and the fixing device.

[0076] The motor 131 drives the lead screw 132 to rotate so that the lead screw 132 drives the adapter block 163 to move along the guide rail 161.

[0077] In an embodiment of the present invention, the system further includes: a stray light removal system 170. As Figure 1a shown, the stray light removal system 170 is installed on the adapter plate 122, and the stray light removal system 170 is used to filter the stray light in the reflected light of the sample to be imaged transmitted by the microscope objective 110.

[0078] As an alternative, if the stray light removal system 170 uses a pinhole confocal system, the stray light removal system further includes a first lens group, a pinhole, and a second lens group. Figure 1b is a schematic structural diagram of a pinhole confocal system provided by an embodiment of the present invention. As Figure 1b shown, the pinhole confocal system includes a first lens group 171, a pinhole 172, and a second lens group 173. The first lens group 171 converges the reflected light of the sample to be imaged to the pinhole 172 and transmits it to the second lens group 173 through the pinhole 172; the second lens group 173 converts the received reflected light into parallel light and transmits the parallel light to the imaging lens group 140.

[0079] In an embodiment of the present invention, the imager 150 includes a detection surface 151; the imaging lens group 140 includes at least one imaging lens, and the imaging lens is used to sequentially converge the reflected light received at different positions to the detection surface 151 and generate a spectral image corresponding to different heights of the sample to be imaged on the detection surface 151.

[0080] In an embodiment of the present invention, the system further includes: a stage 180. As Figure 1a shown, the stage 180 is arranged below the microscope objective 110 and installed on the support assembly 120 to carry the sample to be imaged. Specifically, the sample to be imaged is fixedly placed on the stage 180.

[0081] In an embodiment of the present invention, the system further includes: a controller. The controller is used to obtain the spectral images corresponding to different heights of the sample to be imaged generated on the detection surface 151; and generate a three-dimensional image of the sample to be imaged according to the spectral images of different heights of the sample to be imaged by specifying an imaging algorithm.

[0082] The following uses a specific embodiment to describe in detail the layer imaging process of the sample based on the layer imaging system:

[0083] The sample to be imaged is fixedly placed on the stage 180. The sample to be imaged is made of a luminescent material. The light source is arranged below the stage 180, and the light source irradiates the sample to be imaged on the stage 180; the sample to be imaged emits reflected light (fluorescence / Raman spectrum) towards the microscope objective 110; the microscope objective 110 transmits the reflected light to the stray light removal system 170; the stray light removal system 170 filters out the stray light in the reflected light to remove the light of a specified wavelength that is not needed. Specifically, the stray light removal system 170 can be a band-pass filter of a specified wavelength. When the reflected light is transmitted to the band-pass filter of the specified wavelength, only the light of the specified wavelength is allowed to pass through, thereby filtering out the light of other wavelengths. For example: the wavelength of the excitation light emitted by the light source is 405 nanometers (nm). After being reflected by the sample to be imaged, the wavelength of the reflected light reflected by the sample to be imaged towards the microscope objective 110 is 615 nm. The stray light removal system 170 is a band-pass filter with a specified wavelength of 615 nm. Then the reflected light passes through the stray light removal system 170, and only the light with a wavelength of 615 nm is allowed to pass through the band-pass filter, filtering out the stray light of other wavelengths. By removing the stray light of other wavelengths through the stray light removal system, the resolution of the spectral image and the subsequent generated three-dimensional image can be improved.

[0084] The stray light removal system 170 transmits the filtered light to the imaging lens group 140. The imaging lens group 140 includes at least one imaging lens. The imaging lens can sequentially converge the filtered light received at different positions onto the detection surface 151 of the imager 150, and a spectral image corresponding to different heights of the sample to be imaged is generated on the detection surface 151. Specifically, Figure 2 is a schematic optical path diagram of a layer imaging system provided by an embodiment of the present invention. As Figure 2 shown, according to the optical imaging principle, when the sample to be imaged is located at the focal point of the microscope objective 110, after the reflected light transmitted by the sample to be imaged is collected by the microscope objective 110, the light transmitted by the microscope objective 110 is parallel light, and the detection surface 151 of the imager 150 is located at the focal point of the imaging lens group 140, and a clear image of the sample to be imaged is formed on the detection surface 151 of the imager 150.

[0085] When the sample to be imaged is not located at the focal point of the microscope objective 110, after the reflected light transmitted by the sample to be imaged is collected by the microscope objective 110, the light transmitted by the microscope objective is converging light or diverging light. At this time, by adjusting the distance from the imaging lens group 140 to the detection surface 151 of the imager 150, a clear image of the sample to be imaged can be formed on the detection surface 151 of the imager 150. Figure 3 is another schematic optical path diagram of a layer imaging system provided by an embodiment of the present invention. As Figure 3As shown in the figure, when the sample to be imaged is below the focus of the microscope objective 110, the reflected light transmitted by the sample to be imaged is collected by the microscope objective 110. The light transmitted by the microscope objective 110 is convergent light. The driving device 130 drives the imaging lens group 140 to move upward along the axis direction of the imaging lens group 140 to reduce the distance between the imaging lens group 140 and the detection surface 151 of the imager 150, so that a clear image of the sample to be imaged is formed on the detection surface 151 of the imager 150. Figure 4 This is a schematic optical path diagram of another layer imaging system provided by an embodiment of the present invention. As Figure 4 shown in the figure, when the sample to be imaged is above the focus of the microscope objective 110, the reflected light transmitted by the sample to be imaged is collected by the microscope objective 110. The light transmitted by the microscope objective 110 is divergent light. The driving device 130 drives the imaging lens group 140 to move downward along the axis direction of the imaging lens group 140 to increase the distance between the imaging lens group 140 and the detection surface 151 of the imager 150, so that a clear image of the sample to be imaged is formed on the detection surface 151 of the imager 150.

[0086] A layer imaging system provided by the present invention includes: a microscope objective, a support assembly, a driving device, an imaging lens group, and an imager; the microscope objective, the imaging lens group, and the imager are coaxially installed on the support assembly; the driving device drives the imaging lens group to move along the axis direction of the imaging lens group, so that the imaging lens group converges the received reflected light on the imager to generate spectral images of different heights of the sample to be imaged in turn, performs layer imaging on the sample without moving the sample, and is not restricted by the external environment during the imaging process, thereby improving the accuracy of the three-dimensional image.

[0087] Taking the controller as the execution subject as an example below, the implementation process of the layer imaging method provided by the embodiment of the present invention is described. The layer imaging method provided by the embodiment of the present invention is a specific application based on the above layer imaging system. It can be understood that the execution subject of the layer imaging method provided by the embodiment of the present invention includes but is not limited to the controller.

[0088] Figure 5 This is a flowchart of a layer imaging method provided by an embodiment of the present invention. As Figure 5 shown in the figure, the method includes:

[0089] Step 101, control the driving device to drive the imaging lens group to move along the axis direction of the imaging lens group.

[0090] In an embodiment of the present invention, the driving device is installed on the second fixing plate of the supporting component. The driving device includes a motor and a lead screw, and the lead screw is respectively connected to the motor and the fixing device. The fixing device includes a guide rail, a slider and an adapter block. Among them, the guide rail is fixed on the first fixing plate, the adapter block is fixedly connected to the imaging lens group, the slider is fixedly connected to the adapter block and the slider is arranged in the guide rail and can move along the guide rail.

[0091] Step 102: Control the imager to receive the reflected light converged by the imaging lens group on the imager, so that the imager sequentially generates spectral images of different heights of the sample to be imaged.

[0092] In an embodiment of the present invention, the imaging lens group and the imager are coaxially installed on the supporting component. The supporting component includes an adapter plate, a first fixing plate, a second fixing plate and a third fixing plate. The second fixing plate and the third fixing plate are both installed on the first fixing plate; the imager is installed on the third fixing plate of the supporting component.

[0093] In the technical solution provided by the embodiment of the present invention, control the driving device to drive the imaging lens group to move along the axis direction of the imaging lens group; control the imager to receive the reflected light converged by the imaging lens group on the imager, so that the imager sequentially generates spectral images of different heights of the sample to be imaged, perform layered imaging on the sample without moving the sample, and the imaging process is not restricted by the external environment, thereby improving the accuracy of the three-dimensional image.

[0094] Figure 6 It is a flowchart of another layered imaging method provided by the embodiment of the present invention. As Figure 6 shown, the method includes:

[0095] Step 201: Control the driving device to drive the imaging lens group to move along the axis direction of the imaging lens group.

[0096] In an embodiment of the present invention, the controller can control the driving device to drive the imaging lens group to move along the axis direction of the imaging lens group within a specified moving range according to a specified moving distance threshold. Among them, the specified moving distance threshold is preset. As an optional solution, the specified moving distance threshold is set to 1 μm; the specified moving range is from -200 μm to 300 μm. Specifically, the controller controls the driving device to drive the imaging lens group to move from -200 μm to 300 μm, and collect a spectral image every 1 μm of movement.

[0097] Specifically, if the emitted light transmitted by the microscope objective is divergent light, control the driving device to drive the imaging lens group to move downward along the axis direction of the imaging lens group; if the emitted light transmitted by the microscope objective is convergent light, control the driving device to drive the imaging lens group to move upward along the axis direction of the imaging lens group.

[0098] In an embodiment of the present invention, the microscopic objective lens, the imaging lens group, and the imager are coaxially mounted on a support assembly, and the support assembly includes a microscope bracket, an adapter plate, a first fixing plate, a second fixing plate, and a third fixing plate; the adapter plate is mounted on the top surface of the microscope bracket, the first fixing plate is mounted on the adapter plate, and both the second fixing plate and the third fixing plate are mounted on the first fixing plate; the microscopic objective lens is mounted on the microscope bracket, and the microscope bracket is used to carry the microscopic objective lens.

[0099] In an embodiment of the present invention, since the focal length of the imaging lens group is much larger than the focal length of the microscopic objective lens, when performing layer imaging on the sample to be imaged with the same precision, the moving distance of the driving imaging lens group is much larger than the moving distance of the stage or the microscopic objective lens. Therefore, the requirement for the moving distance of the driving imaging lens group is lower, the control difficulty and cost are reduced, and the layer imaging precision is improved.

[0100] Step 202: Control the stray light removal system to filter the stray light in the reflected light of the sample to be imaged transmitted by the microscopic objective lens.

[0101] In an embodiment of the present invention, the controller controls the stray light removal system to filter out the stray light in the reflected light to remove the light of an unwanted specified wavelength. Specifically, the stray light removal system can be a band-pass filter of a specified wavelength. Transmitting the reflected light to the band-pass filter of the specified wavelength, only the light of the specified wavelength is allowed to pass through, thereby filtering out the light of other wavelengths.

[0102] In an embodiment of the present invention, the stray light removal system is mounted on the adapter plate.

[0103] Step 203: Control the stray light removal system to transmit the filtered light after removing the stray light to the imaging lens group.

[0104] As an alternative solution, the stray light removal system is a pinhole confocal system, and the stray light removal system further includes: a first lens group, a pinhole, and a second lens group. Figure 7 It is a schematic optical path diagram of another layer imaging system provided by an embodiment of the present invention, as Figure 7As shown in the figure, the pinhole confocal system includes: a first lens group, a pinhole, and a second lens group. Specifically, the light source irradiates the sample to be imaged on the stage; the sample to be imaged reflects light (fluorescence / Raman spectrum) towards the microscope objective; the microscope objective transmits the reflected light to the pinhole confocal system; the controller converges the reflected light of the sample to be imaged to the pinhole through the first lens group of the pinhole confocal system, and transmits it to the second lens group through the pinhole; the second lens group converts the received reflected light into parallel light and transmits the parallel light to the imaging lens group; the imaging lens group converges the filtered light received at different positions to the detection surface of the imager in sequence, and generates a spectral image corresponding to different heights of the sample to be imaged on the detection surface. Similarly to adjusting the distance between the imaging lens group and the imager, if the sample to be imaged is below the focus of the microscope objective, after the reflected light transmitted by the sample to be imaged is collected by the microscope objective, the light transmitted by the microscope objective is convergent light. At this time, control the first lens group to move upward along the axis to reduce the distance from the first lens group to the pinhole, so that the reflected light emitted by the sample to be imaged passes through the pinhole; if the sample to be imaged is above the focus of the microscope objective, after the reflected light transmitted by the sample to be imaged is collected by the microscope objective, the light transmitted by the microscope objective is divergent light. At this time, control the first lens group to move downward along the axis to increase the distance from the first lens group to the pinhole, so that the reflected light emitted by the sample to be imaged passes through the pinhole.

[0105] In the embodiment of the present invention, by removing stray light of other wavelengths through the stray light system, the resolution of the spectral image and the subsequent generated three-dimensional image can be improved.

[0106] Step 204: Control the imager to receive the filtered light converged by the imaging lens group to the imager, so that the imager generates spectral images of different heights of the sample to be imaged in sequence.

[0107] In the embodiment of the present invention, according to the optical imaging principle, the imaging lens group converges the filtered light to the imager, and forms a clear image on the detection surface of the imager; by adjusting the distance between the imaging lens group and the imager, the imager generates spectral images of different heights of the sample to be imaged in sequence, that is, performs layer imaging on the sample to be imaged.

[0108] Step 205: Generate a three-dimensional image of the sample to be imaged according to the spectral images of different heights of the sample to be imaged by specifying an imaging algorithm.

[0109] Specifically, input the spectral images of different heights of the sample to be imaged into the specified imaging algorithm, and output the three-dimensional image of the sample to be imaged, which is consistent with the sample to be imaged. Among them, the specified imaging algorithm includes but is not limited to the ICP (Iterative Closest Point) registration algorithm, the MC (Marching Cubes) algorithm, and the MT (Marching Tetrahedra) algorithm.

[0110] It is understandable that the above-mentioned layer imaging method adjusts the distance between the imaging lens group and the imager by controlling the movement of the imaging lens group along the axis direction; it is also possible to adjust the distance between the imaging lens group and the imager by controlling the movement of the imager along the axis direction, and the present invention does not limit this.

[0111] In the embodiments of the present invention, the layer imaging of the sample to be imaged is achieved by adjusting the distance between the imaging lens group and the imager, without moving the stage and the sample to be imaged carried thereon. Therefore, the selection conditions of the sample to be imaged and the stage are no longer restricted by the load and the environment. Larger-volume and larger-weight items can be selected as the sample to be imaged, and a larger vacuum, low-temperature, high-pressure, or high-temperature stage can also be selected as the stage for carrying the sample to be imaged.

[0112] In the technical solution provided by the embodiments of the present invention, the control driving device drives the imaging lens group to move along the axis direction of the imaging lens group; the imager is controlled to receive the reflected light converged by the imaging lens group on the imager, so that the imager sequentially generates spectral images of different heights of the sample to be imaged, and layer imaging of the sample is performed without moving the sample, and the imaging process is not restricted by the external environment, thereby improving the accuracy of the three-dimensional image.

[0113] Figure 8 It is a schematic structural diagram of a layer imaging device provided by an embodiment of the present invention. This device is used to execute the above-mentioned layer imaging method, as Figure 8 shown, this device includes: a first control unit 11 and a second control unit 12.

[0114] The first control unit 11 is used to control the driving device to drive the imaging lens group to move along the axis direction of the imaging lens group.

[0115] The second control unit 12 is used to control the imager to receive the reflected light converged by the imaging lens group on the imager, so that the imager sequentially generates spectral images of different heights of the sample to be imaged.

[0116] In the embodiments of the present invention, the first control unit 11 is specifically used to control the driving device to drive the imaging lens group to move downward along the axis direction of the imaging lens group if the emitted light is divergent light; and to control the driving device to drive the imaging lens group to move upward along the axis direction of the imaging lens group if the emitted light is convergent light.

[0117] In the embodiments of the present invention, this device further includes: a third control unit 13.

[0118] The third control unit 13 is used to control the stray light removal system to filter the stray light in the reflected light of the sample to be imaged transmitted by the microscope objective lens, and transmit the filtered light after removing the stray light to the imaging lens group.

[0119] In an embodiment of the present invention, the device further includes: a converging unit 14 and a conversion unit 15.

[0120] The converging unit 14 is configured to converge the reflected light of the sample to be imaged to a pinhole through a first lens group, and transmit it to a second lens group through the pinhole.

[0121] The conversion unit 15 is configured to convert the received reflected light into parallel light through a second lens group, and transmit the parallel light to an imaging lens group.

[0122] In an embodiment of the present invention, the device further includes: a generating unit 16.

[0123] The generating unit 16 is configured to generate a three-dimensional image of the sample to be imaged according to the spectral images of different heights of the sample to be imaged by a specified imaging algorithm.

[0124] In the solution of the embodiment of the present invention, the control driving device drives the imaging lens group to move along the axis direction of the imaging lens group; the imager is controlled to receive the reflected light converged by the imaging lens group on the imager, so that the imager sequentially generates spectral images of different heights of the sample to be imaged, and the sample is subjected to layer imaging without moving the sample, and the imaging process is not restricted by the external environment, thereby improving the accuracy of the three-dimensional image.

[0125] The systems, devices, modules or units illustrated in the above embodiments may be specifically implemented by computer chips or entities, or by products with certain functions. A typical implementation device is a computer device. Specifically, the computer device may be, for example, a personal computer, a laptop computer, a cellular phone, a camera phone, a smart phone, a personal digital assistant, a media player, a navigation device, an email device, a game console, a tablet computer, a wearable device, or any combination of these devices.

[0126] An embodiment of the present invention provides a computer device, including a memory and a processor. The memory is used to store information including program instructions, and the processor is used to control the execution of the program instructions. When the program instructions are loaded and executed by the processor, the steps of the above embodiment of the layer imaging method are implemented. For specific descriptions, reference may be made to the above embodiment of the layer imaging method.

[0127] Reference is made below to Figure 9 , which shows a schematic structural diagram of a computer device 600 suitable for implementing an embodiment of the present application.

[0128] As Figure 9As shown, the computer device 600 includes a central processing unit (CPU) 601, which can perform various appropriate operations and processes according to programs stored in a read-only memory (ROM) 602 or programs loaded from a storage section 608 into a random access memory (RAM) 603. In the RAM 603, various programs and data required for the operation of the system 600 are also stored. The CPU 601, ROM 602, and RAM 603 are connected to each other via a bus 604. An input / output (I / O) interface 605 is also connected to the bus 604.

[0129] The following components are connected to the I / O interface 605: an input section 606 including a keyboard, a mouse, etc.; an output section 607 including, for example, a cathode ray tube (CRT), a liquid crystal display (LCD), etc. and a speaker, etc.; a storage section 608 including a hard disk, etc.; and a communication section 609 including a network interface card such as a LAN card, a modem, etc. The communication section 609 performs communication processing via a network such as the Internet. A drive 610 is also connected to the I / O interface 606 as needed. A removable medium 611, such as a magnetic disk, an optical disk, a magneto-optical disk, a semiconductor memory, etc., is installed on the drive 610 as needed so that a computer program read therefrom can be installed in the storage section 608 as needed.

[0130] Specifically, according to an embodiment of the present invention, the processes described above with reference to the flowcharts can be implemented as a computer software program. For example, an embodiment of the present invention includes a computer program product that includes a computer program tangibly embodied on a machine-readable medium, the computer program including program code for performing the method shown in the flowchart. In such an embodiment, the computer program can be downloaded and installed from a network via the communication section 609 and / or installed from the removable medium 611.

[0131] A computer-readable medium includes both permanent and non-permanent, removable and non-removable media that can store information by any method or technology. The information can be computer-readable instructions, data structures, program modules, or other data. Examples of computer storage media include, but are not limited to, phase change memory (PRAM), static random access memory (SRAM), dynamic random access memory (DRAM), other types of random access memory (RAM), read-only memory (ROM), electrically erasable programmable read-only memory (EEPROM), flash memory or other memory technologies, compact disc read-only memory (CD-ROM), digital versatile disc (DVD) or other optical storage, magnetic cassettes, magnetic tape, magnetic disk storage or other magnetic storage devices, or any other non-transitory medium that can store information accessible by a computing device. As defined herein, a computer-readable medium does not include transitory computer-readable media such as modulated data signals and carrier waves.

[0132] For convenience of description, when describing the above devices, they are described as various units according to their functions. Of course, when implementing the present application, the functions of each unit can be implemented in one or more software and / or hardware.

[0133] The present invention is described with reference to the flowcharts and / or block diagrams of methods, apparatuses (systems), and computer program products according to embodiments of the present invention. It should be understood that each flow and / or block in the flowcharts and / or block diagrams, and the combination of flows and / or blocks in the flowcharts and / or block diagrams, can be implemented by computer program instructions. These computer program instructions can be provided to the processor of a general-purpose computer, a special-purpose computer, an embedded processor, or other programmable data processing device to generate a machine, such that the instructions executed by the processor of the computer or other programmable data processing device produce a device for implementing the functions specified in one or more flows in the flowchart and / or one or more blocks in the block diagram.

[0134] These computer program instructions can also be stored in a computer-readable memory that can direct a computer or other programmable data processing device to work in a specific manner, such that the instructions stored in the computer-readable memory produce a manufactured article including an instruction device that implements the functions specified in one or more flows in the flowchart and / or one or more blocks in the block diagram.

[0135] These computer program instructions can also be loaded onto a computer or other programmable data processing apparatus, so that a series of operation steps are performed on the computer or other programmable apparatus to produce a computer-implemented process, and thus the instructions executed on the computer or other programmable apparatus provide steps for implementing the functions specified in one process or multiple processes in the flowchart and / or one block or multiple blocks in the block diagram.

[0136] It should also be noted that the term "comprising", "including" or any other variation thereof is intended to cover non-exclusive inclusion, so that a process, method, commodity or device comprising a series of elements includes not only those elements but also other elements not expressly listed, or also includes elements inherent in such process, method, commodity or device. Without further limitation, an element defined by the statement "comprising an..." does not exclude the presence of additional identical elements in the process, method, commodity or device comprising the element.

[0137] Those skilled in the art should understand that the embodiments of the present application can be provided as methods, systems or computer program products. Therefore, the present application can take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects. Moreover, the present application can take the form of a computer program product implemented on one or more computer-usable storage media (including but not limited to disk storage, CD-ROM, optical storage, etc.) containing computer-usable program code.

[0138] The present application can be described in the general context of computer-executable instructions executed by a computer, such as program modules. Generally, program modules include routines, programs, objects, components, data structures, etc. that perform specific tasks or implement specific abstract data types. The present application can also be practiced in a distributed computing environment where tasks are performed by remote processing devices connected through a communication network. In a distributed computing environment, program modules can be located in local and remote computer storage media including storage devices.

[0139] Each embodiment in this specification is described in a progressive manner, and the same or similar parts between the embodiments can be referred to each other. Each embodiment focuses on the differences from other embodiments. In particular, for the system embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the description of the method embodiment.

[0140] The above are only embodiments of the present application and are not intended to limit the present application. For those skilled in the art, various modifications and variations can be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the scope of the claims of the present application.

Claims

1. A layered imaging system, characterized in that, The system includes: a microscopic objective lens, a support assembly, a driving device, an imaging lens group, and an imager; The microscopic objective lens, the imaging lens group, and the imager are coaxially mounted on the support assembly; The driving device drives the imaging lens group to move along the axis direction of the imaging lens group to adjust the distance between the imaging lens group and the imager, so that the imaging lens group converges the received reflected light on the imager to sequentially generate spectral images of different heights of the sample to be imaged; The system further includes: a stage; The stage is arranged below the microscopic objective lens and mounted on the support assembly to carry the sample to be imaged; the sample to be imaged is made of a luminescent material, a light source is arranged below the stage, and the light source irradiates the sample to be imaged on the stage; the sample to be imaged emits reflected light to the microscopic objective lens.

2. The layered imaging system according to claim 1, characterized in that, The support assembly includes: a microscope bracket; The microscopic objective lens is mounted on the microscope bracket.

3. The layered imaging system according to claim 2, wherein The support assembly further includes: an adapter plate, a first fixing plate, a second fixing plate, and a third fixing plate; The adapter plate is mounted on the top surface of the microscope bracket, the first fixing plate is mounted on the adapter plate, and both the second fixing plate and the third fixing plate are mounted on the first fixing plate; The imager is mounted on the third fixing plate; The adapter plate is provided with a light passing hole for the reflected light of the sample to be imaged transmitted by the microscopic objective lens to pass through; The driving device is mounted on the second fixing plate.

4. The layered imaging system according to claim 3, characterized in that, The system further includes: a fixing device; The imaging lens group is connected to the first fixing plate through the fixing device; The fixing device includes a guide rail, a slider, and an adapter block. Among them, the guide rail is fixed on the first fixing plate, the adapter block is fixedly connected to the imaging lens group, and the slider is fixedly connected to the adapter block and the slider is arranged in the guide rail and can move along the guide rail.

5. The layered imaging system according to claim 4, characterized in that, The driving device includes a motor and a lead screw, and the lead screw is respectively connected to the motor and the fixing device; The motor drives the lead screw to rotate so that the lead screw drives the adapter block to move along the guide rail.

6. The layered imaging system according to claim 1, wherein The system further includes: a stray light removal system; The stray light removal system is mounted on the adapter plate, and the stray light removal system is used to filter the stray light in the reflected light of the sample to be imaged transmitted by the microscopic objective lens.

7. The layered imaging system according to claim 6, characterized in that, The stray light removal system further includes: a first lens group, a pinhole, and a second lens group; The first lens group converges the reflected light of the sample to be imaged to the pinhole and transmits it to the second lens group through the pinhole; The second lens group converts the received reflected light into parallel light and transmits the parallel light to the imaging lens group.

8. The layered imaging system according to claim 1, characterized in that, The imager includes a detection surface; the imaging lens group includes at least one imaging lens; The imaging lens is used to sequentially converge the reflected light received at different positions on the detection surface and generate spectral images corresponding to different heights of the sample to be imaged on the detection surface.

9. The layered imaging system according to claim 1, wherein The system further includes: a controller; The controller is used to generate a three-dimensional image of the sample to be imaged according to the spectral images of different heights of the sample to be imaged by specifying an imaging algorithm.

10. A layer imaging method based on a layer imaging system, characterized in that, Applied to the layer imaging system according to any one of claims 1 to 9, the method includes: Controlling a driving device to drive an imaging lens group to move along the axis direction of the imaging lens group to adjust the distance between the imaging lens group and the imager; Controlling the imager to receive the reflected light converged by the imaging lens group onto the imager, so that the imager sequentially generates spectral images of different heights of a sample to be imaged, the sample to be imaged being made of a luminescent material and being carried on a stage; the stage is arranged below the microscope objective and is mounted on the support assembly; a light source is arranged below the stage, and the light source irradiates the sample to be imaged on the stage; the sample to be imaged emits reflected light towards the microscope objective.

11. The layered imaging method according to claim 10, wherein, The controlling the driving device to drive the imaging lens group to move along the axis direction of the imaging lens group includes: If the emitted light is divergent light, controlling the driving device to drive the imaging lens group to move downward along the axis direction of the imaging lens group; If the emitted light is convergent light, controlling the driving device to drive the imaging lens group to move upward along the axis direction of the imaging lens group.

12. The layered imaging method according to claim 10, characterized in that Before the controlling the imager to receive the reflected light converged by the imaging lens group onto the imager, the method further includes: Controlling a stray light removal system to filter the stray light in the reflected light of the sample to be imaged transmitted by the microscope objective and transmit the filtered light after removing the stray light to the imaging lens group.

13. The layered imaging method according to claim 12, wherein The stray light removal system further includes: a first lens group, a pinhole and a second lens group; The method further includes: Converging the reflected light of the sample to be imaged to the pinhole through the first lens group and transmitting it to the second lens group through the pinhole; Converting the received reflected light into parallel light through the second lens group and transmitting the parallel light to the imaging lens group.

14. The layered imaging method according to claim 10, wherein After the controlling the imager to receive the reflected light converged by the imaging lens group onto the imager, so that the imager sequentially generates spectral images of different heights of the sample to be imaged, it further includes: Generating a three-dimensional image of the sample to be imaged according to the spectral images of different heights of the sample to be imaged through a specified imaging algorithm.

15. A layered imaging device, characterized in that, Applied to the layer imaging system according to any one of claims 1 to 9, the device includes: A first control unit for controlling a driving device to drive an imaging lens group to move along the axis direction of the imaging lens group to adjust the distance between the imaging lens group and the imager; A second control unit for controlling the imager to receive the reflected light converged by the imaging lens group onto the imager, so that the imager sequentially generates spectral images of different heights of a sample to be imaged, the sample to be imaged being made of a luminescent material and being carried on a stage; the stage is arranged below the microscope objective and is mounted on the support assembly; a light source is arranged below the stage, and the light source irradiates the sample to be imaged on the stage; the sample to be imaged emits reflected light towards the microscope objective.

16. A computer-readable medium having a computer program stored thereon, characterized in that, When the program is executed by a processor, it implements the layer imaging method according to any one of claims 10 to 14.

17. A computer device, comprising a memory and a processor, the memory being used for storing information including program instructions, and the processor being used for controlling the execution of the program instructions, characterized in that, When the program instructions are loaded and executed by a processor, they implement the layer imaging method according to any one of claims 10 to 14.

Images