A cell image analysis device and a sample analysis method

Abstract

A cell image analysis device and a sample analysis method are provided. An imaging device (101) is used to photograph a sample smear of a sample to be tested in a specified analysis mode to obtain a cell image of the sample to be tested, which is used by a control device (102) to identify and analyze whether the sample smear has an abnormality corresponding to the specified analysis mode. The control device (102) identifies and analyzes the cell image of the sample to be tested. When it is identified that there are other abnormalities in the sample smear that are different from the abnormality corresponding to the specified analysis mode, the analysis mode corresponding to the other abnormality is used as an additional analysis mode, and the imaging device (101) is controlled to photograph the sample smear in the additional analysis mode to further identify and analyze the photographed cell image, thereby realizing automatic additional and calling of the analysis mode. The additional analysis mode matches other abnormalities, so that the imaging device (101) photographs the cell image in the additional analysis mode to identify and analyze the cell image matching other abnormalities, thereby improving the processing efficiency and the accuracy of the processing results.

Description

Technical Field

[0001] The present application relates to medical equipment technology, and in particular to a cell image analysis device and a sample analysis method. Background Art

[0002] Currently, cell image analyzers can process sample smears in a specified analysis mode. This includes sample smear uploading, single cell layer and cell location, cell image acquisition, cell image recognition and analysis, and report generation. To a certain extent, these devices can replace manual microscopic examinations, thereby improving detection speed and accuracy. However, these devices can only process sample smears in a specified analysis mode. Summary of the Invention

[0003] The present application provides a cell image analysis device and a sample analysis method. When a sample smear is processed in a specified analysis mode, if an abnormality is found, an additional analysis mode matching the abnormality is automatically added and the additional analysis mode is called for processing.

[0004] To achieve the above objectives, this application provides the following technical solutions:

[0005] In one aspect, the present application provides a cell image analysis device, comprising:

[0006] An imaging device configured to photograph a sample smear of a sample to be tested in a specified analysis mode to obtain a cell image of the sample to be tested, so as to allow the control device to identify and analyze whether the sample smear has an abnormality corresponding to the specified analysis mode;

[0007] The controls are configured for:

[0008] performing identification and analysis on the cell image of the sample to be tested captured by the imaging device;

[0009] When it is identified that other abnormalities exist in the sample smear that are different from the abnormality corresponding to the specified analysis mode, the analysis mode corresponding to the other abnormality is used as an additional analysis mode;

[0010] The imaging device is controlled to photograph the sample smear in the additional analysis mode, so as to further perform identification analysis on the photographed cell images.

[0011] In another aspect, the present application provides a sample analysis method, comprising:

[0012] Controlling the imaging device to photograph the sample smear of the sample to be tested in a specified analysis mode to obtain a cell image of the sample to be tested, so that the control device can identify and analyze whether the sample smear has an abnormality corresponding to the specified analysis mode;

[0013] performing identification and analysis on the cell image of the sample to be tested captured by the imaging device;

[0014] When it is identified that other abnormalities exist in the sample smear that are different from the abnormality corresponding to the specified analysis mode, the analysis mode corresponding to the other abnormality is used as an additional analysis mode;

[0015] The imaging device is controlled to photograph the sample smear in the additional analysis mode, so as to further perform identification analysis on the photographed cell images.

[0016] On another aspect, the present application provides a storage medium having executable instructions stored thereon, configured to cause a processor to execute the executable instructions to implement the above-mentioned sample analysis method.

[0017] In the present application, an imaging device is used to photograph a sample smear of a sample to be tested in a specified analysis mode to obtain a cell image of the sample to be tested, and a control device is used to identify and analyze the cell image of the sample to be tested. When other abnormalities different from the abnormalities corresponding to the specified analysis mode are identified in the sample smear, the analysis mode corresponding to the other abnormalities is used as an additional analysis mode, and the imaging device is controlled to photograph the sample smear in the additional analysis mode to further identify and analyze the photographed cell image. When the sample smear is processed in the specified analysis mode, if other abnormalities different from the abnormalities corresponding to the specified analysis mode are found, the additional analysis mode matching the other abnormalities is automatically added to further process the sample smear, thereby realizing automatic addition and call of the analysis mode. The additional analysis mode matches the other abnormalities found, so that the imaging device can photograph the cell images matching the other abnormalities under the guidance of the additional analysis mode to identify and analyze the cell images matching the other abnormalities, thereby improving processing efficiency and the accuracy of the processing results. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without any creative work.

[0019] Figure 1 Schematic diagram of the structure of a cell image analysis device provided in an embodiment of the present application;

[0020] Figure 2 It is Figure 4 This is a schematic diagram of an optional shooting method for the blood leukocyte analysis mode provided in an embodiment of the present application;

[0021] Figure 5 This is a schematic diagram of an optional shooting method for the red blood cell analysis mode provided in an embodiment of the present application;

[0022] Figure 6 This is a schematic diagram of an optional shooting method for the blood platelet analysis mode provided in an embodiment of the present application;

[0023] Figures 7 to 9 This is a schematic diagram of an optional shooting method for the body fluid leukocyte analysis mode provided in an embodiment of the present application;

[0024] Figure 10 This is a schematic diagram of an optional shooting method for the whole-cell analysis mode provided in the embodiments of the present application;

[0025] Figure 11 This is an optional schematic diagram of a sample smear provided in an embodiment of the present application;

[0026] Figure 12 is a schematic structural diagram of another cell image analysis device provided in an embodiment of the present application;

[0027] Figure 13 and Figure 14 Schematic diagram of the structure of another cell image analysis device provided in an embodiment of the present application;

[0028] Figure 15 This is a schematic structural diagram of a control device in the cell image analysis device provided in an embodiment of the present application;

[0029] Figure 16 This is a flow chart of a sample analysis method provided in an embodiment of the present application. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solutions and advantages of this application more clear, this application will be further described in detail below with reference to the accompanying drawings. This application should not be understood as being limited to the embodiments provided. On the contrary, the contents described in the embodiments of this application make this application comprehensive and complete, and convey the concepts of the embodiments of this application to those skilled in the art. Therefore, other embodiments obtained by ordinary technicians in this field without making creative efforts are all within the scope of protection of this application.

[0031] It should be noted that, in the embodiments of the present application, the terms "comprise," "include," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a method or apparatus comprising a series of elements includes not only the elements explicitly stated, but also other elements not explicitly listed, or elements inherent to the implementation of the method or apparatus. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other related elements in the method or apparatus comprising the element.

[0032] For example, the cell image analysis device provided in the embodiments of the present application includes a series of devices, but the cell image analysis device provided in the embodiments of the present application is not limited to including the devices explicitly described. Similarly, the sample analysis method provided in the embodiments of the present application includes a series of steps, but the sample analysis method provided in the embodiments of the present application is not limited to the described steps. It should be noted that in the following description, reference is made to "embodiments", which describe a subset of all possible embodiments, but it is understood that "embodiments" can be the same subset or different subsets of all possible embodiments, and can be combined with each other without conflict.

[0033] See also Figure 1 , which shows an optional structure of a cell image analysis device provided in an embodiment of the present application, which may include: an imaging device 101 and a control device 102.

[0034] The imaging device 101 is configured to capture a sample smear of a test sample in a specified analysis mode, obtaining a cell image of the test sample, which is then used by the control device to identify and analyze whether the sample smear contains abnormalities corresponding to the specified analysis mode. The test sample can be a blood sample, a body fluid sample, or other sample requiring re-examination by the cell image analyzer.

[0035] The sample to be tested can be any sample specified by the user, including blood samples and body fluid samples. The sample to be tested can also be a sample that needs to be re-examined and re-drawn after various cell count tests (such as blood routine tests) are screened out by a blood cell analyzer. The screening of the sample can be performed by the control device 102 or a device that can communicate with the cell image analysis device according to the test results of the sample by the blood cell analyzer. One of the screening methods is: screening the sample to be tested according to the test results of the sample and the preset re-examination conditions. When the test result of the sample meets the preset re-examination conditions, the sample to which the test result belongs will be re-drawn and determined as the sample to be tested.

[0036] The preset re-examination conditions are used to indicate that the test results include abnormal results indicating that the sample is abnormal, the test results include specific types of cell parameters, the subject to which the test results belong is a preset object of attention, and the sample corresponding to the test results comes from at least one of the specific departments. The above-mentioned specific types of cell parameters include but are not limited to the white blood cell parameter. The preset object of attention can be but is not limited to patients with white blood cell diseases. The specific department can be but is not limited to reproductive medicine, etc. The specific types of cell parameters, preset objects of attention and specific departments can be determined according to medical needs, and this embodiment does not limit them.

[0037] For example, the preset retest condition includes WBC (white blood cell) <2. If the WBC count value of a sample after being tested by a blood cell analyzer is 1, the unit of the WBC count value is 10. 9 / L, the test result of the sample triggers the preset re-inspection condition, and the sample needs to be re-inspected by the cell image analysis device.

[0038] After determining the sample to be tested, the cell image analysis device can obtain the sample smear of the sample to be tested prepared by the smear preparation device, and the imaging device 101 in the cell image analysis device photographs the sample smear of the sample to be tested under the specified analysis mode, wherein the specified analysis mode at least indicates the shooting conditions when the imaging device 101 photographs the sample smear, and the shooting conditions are used to indicate at least one of the shooting position, shooting area and number of images taken when photographing the sample smear, so as to indicate that the cell image photographed by the imaging device 101 is consistent with the cell image required by the specified analysis mode.

[0039] Methods for determining the specified analysis mode include but are not limited to the following two methods:

[0040] One approach is for the control device 102 to determine the analysis mode selected by the user as the designated analysis mode. For example, the cell image analyzer may also include a human-computer interaction device, such as a display screen or camera, which responds to human-computer interaction instructions to pre-set the designated analysis mode through user intervention before the sample smear is captured. Alternatively, the user-selected analysis mode is remotely transmitted to the control device 102. Remote transmission refers to a communication connection between a terminal for selecting the analysis mode and the cell image analyzer, where the user selects the analysis mode on the terminal and then transmits the selected analysis mode to the control device. For example, the user may designate both the white blood cell analysis mode and the red blood cell analysis mode as the designated analysis mode, or the user may designate only the white blood cell analysis mode as the designated mode.

[0041] Another method is: the control device 102 calls the analysis mode based on at least one of the test result information of the sample to be tested and the sample information of the sample to be tested, and determines the called analysis mode as the designated analysis mode, thereby automatically determining the designated analysis mode. The test result information of the sample to be tested can indicate an abnormality in the sample to be tested, and then the analysis mode that matches the abnormality is called as the designated analysis mode. The test result information of the sample to be tested can be the test result information output by a blood cell analyzer for testing the sample. The sample information of the sample to be tested includes: at least one of sample type information and subject information, and the matching analysis mode is selected as the designated analysis mode based on at least one of the sample type information and subject information.

[0042] The control device 102 is configured to:

[0043] Identify and analyze the cell image of the sample to be tested captured by the imaging device;

[0044] When other abnormalities are identified in the sample smear that are different from the abnormalities corresponding to the specified analysis mode, the analysis mode corresponding to the other abnormalities is used as an additional analysis mode. It should be noted that the other abnormalities in this application can be suspected abnormalities of the pointed object or abnormalities of the pointed object.

[0045] The imaging device is controlled to photograph the sample smear in the additional analysis mode to further identify and analyze the photographed cell images.

[0046] The abnormality corresponding to the designated analysis mode and the other abnormality are two different types of abnormalities and can be directed to different analysis modes. The two different types of abnormalities can be abnormalities for different cell types. For example, if the abnormality corresponding to the designated analysis mode is for white blood cells and the other abnormality is for red blood cells, the control device 102 can continue to select the analysis mode corresponding to the other abnormality as an additional analysis mode.

[0047] The abnormalities corresponding to the above-mentioned designated analysis mode and other abnormalities may be abnormalities for the same type of cells but can correspond to different analysis modes. For example, the abnormality corresponding to the designated analysis mode is for white blood cells, and the other abnormalities are also for white blood cells, but the analysis mode corresponding to the other abnormalities at this time is not the designated analysis mode. At this time, the control device 102 will continue to select the analysis mode corresponding to the other abnormalities as the additional analysis mode.

[0048] If the anomaly corresponding to the specified analysis mode and other anomalies can point to different analysis modes, the additional analysis mode and the specified analysis mode are different in name, and at least one of the corresponding shooting conditions and analysis requirements under the additional analysis mode and the specified analysis mode is also different. In this case, the control device 102 will control the imaging device to shoot according to the shooting conditions and / or analysis requirements under the additional analysis mode.

[0049] After the imaging device 101 captures a cell image in the additional analysis mode, the control device 102 will continue to identify and analyze the cell image. If other abnormalities different from the abnormalities corresponding to the specified analysis mode and the additional analysis mode are detected in the sample smear during the identification and analysis process, the control device 102 will further select the analysis mode corresponding to the other abnormality as another additional operation mode, and then control the imaging device 101 to continue capturing in another additional analysis mode.

[0050] In this embodiment, the control device can control the imaging device in such a way that, after the imaging device completes the sample smear shooting in the specified analysis mode, it continues to call the additional analysis mode to shoot the sample smear, that is, the imaging device will shoot in the specified analysis mode and the additional analysis mode in sequence, so that it can re-examine multiple abnormalities in the sample to be tested in one re-examination, thereby improving the processing effect and the accuracy of the processing results.

[0051] When the imaging device obtains cell images in both the designated analysis mode and the supplemental analysis mode, the cell images captured in the designated analysis mode and the cell images captured in the supplemental analysis mode are displayed differently, thereby distinguishing the cell images captured in the designated analysis mode from the cell images captured in the supplemental analysis mode. For example, the cell images captured in the designated analysis mode may be displayed in a standard image display mode, while the cell images captured in the supplemental analysis mode may be displayed in at least one of a highlighted display, a preset color border, the cell image name, and a preset text format. Of course, the cell images in the designated analysis mode may also be displayed in a highlighted display mode, while the cell images in the supplemental analysis mode may be displayed normally.

[0052] and / or

[0053] The recognition and analysis results corresponding to the specified analysis mode and the recognition and analysis results corresponding to the supplementary analysis mode are displayed differently. For example, the recognition and analysis results corresponding to the specified analysis mode and the recognition and analysis results corresponding to the supplementary analysis mode may use at least one different text color, text format, or text font. For example, the recognition and analysis results corresponding to the specified analysis mode may use black text, while the recognition and analysis results corresponding to the supplementary analysis mode may use red text.

[0054] As can be seen from the description of the cell image analysis device described above, the imaging device 101 photographs a sample smear of the sample to be tested in a specified analysis mode to obtain a cell image of the sample to be tested. The control device 102 identifies and analyzes the cell image of the sample to be tested. If an abnormality other than the abnormality corresponding to the specified analysis mode is identified in the sample smear, the analysis mode corresponding to the abnormality is used as an additional analysis mode, and the imaging device 101 is controlled to photograph the sample smear in the additional analysis mode to further identify and analyze the photographed cell image. This allows, when processing the sample smear in the specified analysis mode, if a different abnormality is found, the additional analysis mode matching the other abnormality is automatically added to further process the sample smear, thereby achieving automatic addition and invocation of analysis modes. The additional analysis mode matches the other abnormality found, allowing the imaging device to capture cell images matching the other abnormality under the guidance of the additional analysis mode, and to identify and analyze the cell images matching the other abnormality, thereby improving processing efficiency and the accuracy of processing results.

[0055] In this embodiment, the designated analysis mode and the additional analysis mode may include one or more analysis modes. The designated analysis mode and the additional analysis mode are expressed as follows:

[0056] The designated analysis modes include at least one of: a white blood cell analysis mode for imaging and analyzing white blood cells in a blood sample smear; a red blood cell analysis mode for imaging and analyzing red blood cells in a blood sample smear; a platelet routine analysis mode for imaging and analyzing platelets in a blood sample smear; a body fluid white blood cell analysis mode for imaging and analyzing white blood cells in a body fluid sample smear; a platelet aggregation analysis mode for imaging platelets in a blood sample smear to identify and analyze their aggregation; an abnormal white blood cell analysis mode for imaging white blood cells in a blood sample smear to identify and analyze giant abnormal cells; a body fluid red blood cell analysis mode for imaging and analyzing red blood cells in a body fluid sample smear; and a whole cell analysis mode for imaging and analyzing all cells within a designated area of ​​a sample smear. It should be noted that giant abnormal white blood cells include at least megakaryocytes and giant immature white blood cells.

[0057] The additional analysis mode is one or more of: blood white blood cell analysis mode, blood red blood cell analysis mode, blood platelet routine analysis mode, body fluid white blood cell analysis mode, body fluid red blood cell analysis mode, whole cell analysis mode, abnormal white blood cell analysis mode and platelet aggregation analysis mode. The additional analysis mode is different from the specified analysis mode and is an analysis mode corresponding to other abnormalities.

[0058] In the analysis modes of blood white blood cell analysis mode, blood red blood cell analysis mode, blood platelet routine analysis mode, body fluid white blood cell analysis mode, body fluid red blood cell analysis mode, and whole cell analysis mode, the imaging device 101 photographs the sample smear in the following manner:

[0059] Blood leukocyte analysis mode:

[0060] The imaging device 101 searches and determines a shooting area using a low-magnification objective lens (e.g., a 10x objective lens) of the first objective lens and the second objective lens. When searching and determining the shooting area, the low-magnification objective lens may move from the head to the tail of the sample smear to determine the shooting area. The determined shooting area may be, but is not limited to, a single cell layer area at the junction of the tail and body of the sample smear (also referred to as the body-tail junction). The determined shooting area may be one or more areas.

[0061] The white blood cells in the shooting area are scanned and located by a low-magnification objective lens. During the scanning and locating process of the white blood cells, the low-magnification objective lens can be scanned and located in a battlement-type manner, such as moving the low-magnification objective lens from a first direction to a second direction for scanning and then moving from the second direction to the first direction for scanning, and repeating the above multiple times to scan and locate various positions in the shooting area, wherein the first direction to the second direction and the second direction to the first direction can be the vertical direction from the head to the tail of the sample smear; the scanned and located white blood cells are photographed by a high-magnification objective lens (such as a 100x objective lens) among the first and second objective lenses of the imaging device.

[0062] like Figures 2 to 4 As shown, the 10x objective lens in the imaging device automatically searches from the head to the tail to determine the shooting area of ​​the sample smear, as shown in FIG. Figure 2 The arrow in the figure shows the direction of automatic search from the head to the tail; then the 10x objective lens is used to Figure 3 The battlement-type method shown is used to scan and locate the white blood cells in the shooting area; after the white blood cells are scanned and located, the scanned and located white blood cells are photographed through a 100x objective lens, as shown in FIG. Figure 4 As shown, the image clarity of the visual field is improved under the 100x objective lens. The leukocytes located by the scanning are magnified by the 100x objective lens and then photographed by the camera in the imaging device to obtain Figure 10 Magnified images of leukocytes are shown.

[0063] Blood red blood cell analysis mode:

[0064] The imaging device searches and determines a shooting area using a low-magnification objective lens (e.g., a 10x objective lens) among the first and second objective lenses. When searching and determining the shooting area, the low-magnification objective lens can move from the head to the tail of the sample smear to search and determine the shooting area. The determined shooting area can be, but is not limited to, a single-layer cell area at the junction of the tail and body of the sample smear (referred to as the body-tail junction). The determined shooting area can be one or more areas. The red blood cells in the shooting area are photographed using a high-magnification objective lens (e.g., a 100x objective lens) among the first and second objective lenses of the imaging device.

[0065] like Figure 5 As shown, a shooting area is determined under a 10x objective lens (as shown in the box in Figure 5), and then the shooting area is photographed under a 100x objective lens, or a shooting area is set in the shooting area and then the area covered by the shooting area is photographed under a 100x objective lens. When shooting under a 100x objective lens, multiple images may be captured, and there may be overlap between adjacent images. All images under a 100x objective lens are spliced ​​to obtain Figure 5 Image of the captured area shown.

[0066] Routine analysis mode of blood platelets:

[0067] The imaging device searches and determines a shooting area using a low-magnification objective lens (e.g., a 40x objective lens) of the first objective lens and the second objective lens. When searching and determining the shooting area, the low-magnification objective lens may move from the head to the tail of the sample smear to determine the shooting area. The determined shooting area may be, but is not limited to, a single layer of cells at the junction of the body and tail of the sample smear. The determined shooting area may be one or more areas.

[0068] Platelets in the imaging area are photographed using a high-magnification objective lens (e.g., a 100x objective lens) of the first and second objective lenses of the imaging device. The reason for using a 40x objective lens to search and determine the imaging area in the conventional blood and platelet analysis mode is that platelets are relatively small. Using a 10x objective lens to search may mistakenly identify dust particles, etc., that are similar in size to platelets, as platelets. This could result in incorrect cell images being captured in the conventional blood and platelet analysis mode, reducing accuracy.

[0069] like Figure 6 As shown, determine the shooting area (as shown in the box in Figure 6) under the 40x objective lens, and then use the 100x objective lens to Figure 6 You can shoot the shooting area shown in the figure, or set the shooting area in the shooting area and then shoot the area covered by the shooting area under the 100x objective lens. When shooting under the 100x objective lens, multiple images may be captured, and there may be overlap between adjacent images. All images under the 100x objective lens are stitched together to obtain Figure 6Image of the captured area shown.

[0070] Body fluid leukocyte analysis mode:

[0071] Determine the photographing area of ​​the sample smear. For body fluid samples, when preparing a sample smear of the body fluid sample, it is necessary to swing out an area of ​​a specific shape in the sample smear. Therefore, in the body fluid leukocyte analysis mode, the photographing area of ​​the sample smear of the body fluid sample needs to be based on the area of ​​the specific shape in the sample smear, such as specifying a photographing area within the area of ​​the specific shape in the sample smear. The photographing position and photographing size of the photographing area can be determined by human-computer interaction, such as by allowing the user to specify a photographing position within the area of ​​the specific shape in the sample smear through human-computer interaction on a display interface, and specifying a photographing size extending outward based on the photographing position, thereby obtaining the photographing area. Alternatively, the above-mentioned photographing area can cover the area of ​​the specific shape in the sample smear, such as the photographing area includes the area of ​​the specific shape in the sample smear or includes a portion of the area of ​​the specific shape in the sample smear, etc.

[0072] The white blood cells in the shooting area are scanned and located by a low-magnification objective lens (such as a 10x objective lens) among the first objective lens and the second objective lens of the imaging device. During the scanning and locating of the white blood cells, the low-magnification objective lens can be scanned and located in a battlement-type manner, such as moving the low-magnification objective lens from a first direction to a second direction for scanning and then moving from the second direction to the first direction for scanning, and this is repeated multiple times to scan and locate various positions in the shooting area, wherein the first direction to the second direction and the second direction to the first direction can be vertical directions from the head to the tail of the sample smear; the scanned and located white blood cells are photographed by a high-magnification objective lens (such as a 100x objective lens) among the first objective lens and the second objective lens of the imaging device.

[0073] like Figures 7 to 9 As shown, the imaging device obtains the user's Figure 7 The designated shooting area on the sample smear is located in a specific shape area of ​​the sample smear (eg Figure 7 In the circular area shown in FIG, the 10x objective lens of the imaging device is used to Figure 8 The battlement-type scanning method is shown, and the white blood cells in the shooting area are then magnified by a 100x objective lens, and the camera in the imaging device is used to shoot the white blood cells. Figure 9 Magnified images of leukocytes are shown.

[0074] Body fluid red blood cell analysis mode:

[0075] Determine the shooting area of ​​the sample smear; shoot the shooting area using a high-power objective lens (such as a 100x objective lens) in the first objective lens and the second objective lens of the imaging device. For the shooting method of the body fluid red blood cell analysis mode, please refer to the above-mentioned blood red blood cell analysis mode.

[0076] Whole-cell analysis mode:

[0077] Determine a photographic area for the sample smear, where the photographic area can be a fixed area, the position of which can be pre-set or determined based on an image captured by a low-magnification objective lens (such as the first objective lens described above) in an imaging device. Alternatively, the photographic area can be a variable area, for example, the photographic area can be determined through human-computer interaction, such as by a user specifying a photographic position and size (the photographic position and size define the photographic area), or by a user specifying a photographic position, and a photographic area automatically determined based on the photographic position, such as a circular or rectangular area centered on the photographic position. Human-computer interaction methods may include, but are not limited to, human-computer interaction via a display interface, human-computer interaction commands sent via voice, and the like.

[0078] After determining the shooting area, the high-power objective lens (e.g., a 100x objective lens) of the first and second objective lenses in the imaging device performs a full-area scan of the shooting area to obtain an image of the shooting area under the high-power objective lens. Full-area scanning means scanning every position in the shooting area. When the high-power objective lens performs a full-area scan of the shooting area, due to the high magnification of the high-power objective lens, it needs to perform multiple scans to complete the full-area scan. Therefore, each scan of the high-power objective lens obtains an image of the scanned area. After the last scan is completed, the images obtained each time are spliced ​​in the order of the scans to form an image of the shooting area under the high-power objective lens.

[0079] like Figure 10 The figure shows the shooting in the whole cell analysis mode. First, the shooting area is determined, and then the shooting area is scanned in the whole area under the 100x objective lens. Multiple images will be obtained during the whole area scanning process. After the whole area scanning is completed, the multiple images are spliced ​​into a Figure 10 In actual medical treatment, one or more imaging regions can be determined in whole-cell analysis mode, and each imaging region can be fully scanned to obtain a stitched image of each imaging region. If all imaging regions need to be stitched into a single image, the images of each imaging region can be stitched together to obtain a single image corresponding to all imaging regions.

[0080] The above describes the shooting process of the imaging device 101 in any analysis mode. In some cases, the imaging device 101 can shoot at least two shooting areas in one analysis mode. For example, if the designated analysis mode is at least one of a blood platelet aggregation analysis mode and an abnormal white blood cell analysis mode, the control device 102 is further configured to determine a first shooting area in the blood platelet analysis mode and a second shooting area different from the first shooting area.

[0081] An imaging device is used to photograph a first photographing area and a second photographing area of ​​a sample smear in a blood and platelet analysis mode to obtain cell images, so that the imaging device can photograph the first photographing area and the second photographing area sequentially or simultaneously in the blood and platelet analysis mode to obtain cell images of two different photographing areas, thereby enabling a re-examination of cell images of more photographing areas.

[0082] In the blood platelet aggregation analysis mode and abnormal white blood cell analysis mode, the imaging device's shooting process is as follows:

[0083] A shooting method in blood platelet aggregation analysis mode:

[0084] The imaging device searches and determines a shooting area using a low-magnification objective lens (e.g., a 40x objective lens) of the first objective lens and the second objective lens. When searching and determining the shooting area, the low-magnification objective lens may move from the head to the tail of the sample smear to determine the shooting area. The determined shooting area may be, but is not limited to, at least two areas of a single cell layer area at the junction of the body and tail of the sample smear, the tail of the sample smear, and the edge of the sample smear.

[0085] The platelets in the photographing area are photographed by a high-magnification objective lens (such as a 100-magnification objective lens) among the first objective lens and the second objective lens of the imaging device.

[0086] Another way to shoot in blood platelet aggregation analysis mode:

[0087] The imaging device uses the higher-magnification objective lens (e.g., a 100x objective lens) of the first and second objective lenses to determine the capture area while scanning. For example, if a sample smear is scanned from the center toward the edge, the edge's position can be determined in real time based on the image, allowing the image to be captured. Similarly, if a blood smear is scanned from the body-tail junction toward the tail, the tail's position can be determined in real time based on the image, allowing the image to be captured.

[0088] In the two aforementioned blood platelet aggregation analysis modes, the imaging device may capture a larger area, thereby increasing the duration of the imaging device in the blood platelet aggregation analysis mode. However, the larger capture area allows the imaging device to obtain a more comprehensive cell image, thereby improving accuracy. While the conventional blood platelet analysis mode reduces the duration compared to the blood platelet aggregation analysis mode, it also reduces accuracy.

[0089] A shooting method in abnormal white blood cell analysis mode:

[0090] The imaging device searches and determines a shooting area using a low-magnification objective lens (e.g., a 10x objective lens) of the first objective lens and the second objective lens. When searching and determining the shooting area, the low-magnification objective lens may move from the head to the tail of the sample smear to determine the shooting area. The determined shooting area may be, but is not limited to, at least two areas of a single cell layer area at the junction of the body and tail of the sample smear, the tail of the sample smear, and the edge of the sample smear.

[0091] The white blood cells in the shooting area are photographed by a high-power objective lens (such as a 100-power objective lens) among the first objective lens and the second objective lens of the imaging device.

[0092] Another way to shoot in abnormal white blood cell analysis mode:

[0093] The imaging device determines the capture area while scanning using the higher-magnification objective lens (e.g., a 100x objective lens) of the first and second objective lenses. For example, scanning from the center of a sample smear toward its edge allows the edge's position to be determined in real time based on the image, allowing the image to be captured. Similarly, scanning from the junction of the body and tail of a sample smear toward its tail allows the tail's position to be determined in real time based on the image, allowing the image to be captured.

[0094] The platelet aggregation analysis mode and the abnormal white blood cell analysis mode are introduced into the designated analysis mode and the supplementary analysis mode. This allows direct selection of at least one of the platelet aggregation analysis mode and the abnormal white blood cell analysis mode within the designated analysis mode. Alternatively, if platelet abnormalities are detected in a cell image captured in the designated analysis mode, the platelet aggregation analysis mode can be selected as the supplementary analysis mode. Specifically, if platelet abnormalities are detected in a sample smear indicating an abnormality other than the one corresponding to the designated analysis mode, the control device 102 selects the platelet aggregation analysis mode as the supplementary analysis mode. Furthermore, if at least one of giant abnormal white blood cells, such as megakaryocytes and giant immature cells, is detected in the designated analysis mode, the abnormal white blood cell analysis mode can be selected as the supplementary analysis mode. Specifically, if giant abnormal white blood cells are detected in a sample smear indicating an abnormality other than the one corresponding to the designated analysis mode, the abnormal white blood cell analysis mode can be selected as the supplementary analysis mode.

[0095] Platelet abnormalities include at least one of platelet aggregation and low platelet count (such as platelet count value lower than the normal range). Platelet aggregation is used to indicate that platelets in the sample to be tested are aggregated (such as concentrated in a certain area). For platelet abnormalities, it is necessary to guide the imaging device to shoot through the blood platelet aggregation analysis mode to identify and analyze whether there is indeed severe platelet aggregation, so as to determine whether the platelet abnormality is caused by platelet aggregation, especially whether the low platelet count is caused by platelet aggregation, so as to determine whether the low platelet count is a false decrease.

[0096] Relative to the designated analysis mode, the analysis requirements in the supplementary analysis mode may differ from those in the designated analysis mode, and the imaging area in the supplementary analysis mode may also differ from the imaging area in the designated analysis mode. Specifically, the control device 102 is further configured to determine a second imaging area in the supplementary analysis mode that is different from the first imaging area, and instruct the imaging device to image the second imaging area in the sample smear, where the first imaging area is the imaging area in the sample smear in the designated analysis mode. The corresponding imaging device 101 further images the second imaging area in the sample smear in the supplementary analysis mode to obtain a cell image of the second imaging area, and the control device performs recognition and analysis on the cell image of the second imaging area.

[0097] The first shooting area and the second shooting area are two different shooting areas in the sample smear. The schematic diagram of each part in the blood film area of ​​the sample smear is as follows: Figure 11 As shown, the regions of a sample smear include: the head, the body, the junction of the body and the tail, the tail, and the edge. The first shooting area and the second shooting area can be at least one of these areas. Then, the difference between the first shooting area and the second shooting area means that the first shooting area and the second shooting area are two completely independent shooting areas, such as the first shooting area is the head and the second shooting area is the tail; or the difference between the first shooting area and the second shooting area means that one of the first shooting area and the second shooting area is larger than the other, such as the first shooting area is the head and the second shooting area is the head and the body; or the difference between the first shooting area and the second shooting area means that part of the first shooting area and the second shooting area overlap, such as the first shooting area is the head and the body, and the second shooting area is the body and the junction of the body and the tail.

[0098] In this embodiment, the first shooting area and the second shooting area may be set in a manner that: at least one of the first shooting area and the second shooting area is set in advance, such as the first shooting area is set in advance by a user, and the second shooting area is obtained based on the first shooting area. One manner of obtaining the second shooting area based on the first shooting area is that: the second shooting area is extended from the first shooting area in a specified direction, and the specified direction can indicate that the imaging device captures a cell image that matches other abnormalities, that is, the captured cell image may indicate other abnormalities. For example, when the other abnormality is platelet aggregation, it is necessary to capture the tail and / or edge of the sample smear, and then the specified direction is the tail and / or edge.

[0099] The first imaging area in the above-mentioned designated analysis mode can be the junction of the body and tail of the sample smear, and the corresponding second imaging area is obtained by extending from the junction of the body and tail toward at least one of the tail and edge of the sample smear in a designated direction. The second imaging area is extended to or close to at least one of the tail and edge corresponding to the body and tail junction of the sample smear, so that the imaging device can image the tail of the sample smear and / or the edge corresponding to the body and tail junction. The reason for extending toward at least one of the tail and edge is to determine whether the abnormality of the test sample is caused by the concentration of cells in the test sample at the tail and / or edge by capturing cell images at the tail and / or edge of the sample smear. If the cells are concentrated at the tail and / or edge, other abnormalities other than the abnormality corresponding to the designated analysis mode can be reviewed based on the concentration of cells at the tail and / or edge.

[0100] It should be noted that there are edges at the head, body, junction of body and tail, and tail of the sample smear. When the edge is selected as the second shooting area, the second shooting area may include at least one of the tail and the edge of the sample smear. The edge of the sample smear may be all edges or edges on both sides of the junction of body and tail, such as Figure 11 At least one of the two edges shown, while the other edge is not, serves as the second photographing area.

[0101] The second shooting area is automatically determined in the above manner, and cell images matching other abnormalities can be obtained through the automatically determined second shooting area, so that other abnormalities can be reviewed by identifying and analyzing the cell images.

[0102] In this embodiment, the second shooting area can also be set in other ways, such as Figure 12 As shown, it shows an optional structure of another cell image analysis device provided in the embodiment of the present application. Figure 1 Furthermore, the present invention may further include: a display device 103 .

[0103] The display device 103 is configured to display the sample smear. The control device 102 is further configured to obtain a user-selected imaging area from the displayed sample smear and use the selected imaging area as a second imaging area. The second imaging area is set by the user. For example, if the user selects an imaging area from the displayed sample smear, the selected second imaging area is different from the first imaging area in the specified analysis mode. The difference between the first imaging area and the second imaging area can be seen in the above description.

[0104] In this embodiment, the display device 103 can also display a first shooting area in the sample smear during the display of the sample smear, so that the user can select a second shooting area with reference to the first shooting area. For example, the first shooting area is the junction of the body and tail of the sample smear, and the shooting area selected by the user (i.e., the second shooting area) is at least one area between the tail of the sample smear and the edge of the sample smear.

[0105] Because the edge of the sample smear may cover a relatively large number of cells, photographing the entire edge of the sample smear will increase working time and increase cell images unrelated to other abnormalities. For this reason, in this embodiment, the edge of the sample smear serving as the second photographing area can be the edge corresponding to the junction of the body and tail in the sample smear. For the description of the edge corresponding to the junction of the body and tail, please refer to the above description.

[0106] Through the above Figure 12 The cell image analysis device shown enables the user to intervene and select the second shooting area so that the second shooting area can match other abnormalities, thereby increasing the probability of other abnormalities existing in the captured cell image and improving shooting accuracy.

[0107] In addition, the above Figure 12 The display device in the cell analysis device shown can also display the cell images captured by the imaging device, so that the user can browse and manually review the cell images.

[0108] The following describes the selection of additional analysis modes using a blood sample or a body fluid sample as the sample to be tested. The control device 102 determines the analysis modes corresponding to other abnormalities for the blood sample as follows, so that analysis modes matching the cell types are called for abnormalities of different cell types in the blood sample:

[0109] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the blood leukocyte analysis mode, the blood leukocyte analysis mode is the analysis mode corresponding to the other abnormalities;

[0110] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the red blood cell analysis mode, the red blood cell analysis mode is the analysis mode corresponding to the other abnormalities;

[0111] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the blood and platelet routine analysis mode, the blood and platelet routine analysis mode is the analysis mode corresponding to the other abnormalities;

[0112] If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in the blood platelet aggregation analysis mode, the blood platelet aggregation analysis mode is the analysis mode corresponding to the other abnormality;

[0113] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the whole-cell analysis mode, the whole-cell analysis mode is the analysis mode corresponding to the other abnormalities;

[0114] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the abnormal white blood cell analysis mode, the abnormal white blood cell analysis mode is the analysis mode corresponding to the other abnormalities.

[0115] The control device 102 determines the analysis modes corresponding to other abnormalities for the body fluid sample as follows, so as to call the analysis mode matching the cell type for the abnormalities of different cell types in the body fluid sample:

[0116] If the sample to be tested is a body fluid sample and the other abnormalities correspond to abnormalities in the body fluid leukocyte analysis mode, the body fluid leukocyte analysis mode is the analysis mode corresponding to the other abnormalities;

[0117] If the sample to be tested is a body fluid sample and the other abnormalities correspond to abnormalities in the body fluid red blood cell analysis mode, the body fluid red blood cell analysis mode is the analysis mode corresponding to the other abnormalities;

[0118] If the sample to be tested is a body fluid sample and the other abnormalities correspond to abnormalities in the whole-cell analysis mode, the whole-cell analysis mode is the analysis mode corresponding to the other abnormalities.

[0119] The following describes the abnormalities that can be identified by the cell image analysis device, as shown in Tables 1 and 2, which illustrate the abnormalities identified when identifying white blood cell images and red blood cell images:

[0120] Table 1 White blood cell abnormalities identified during cell image recognition of white blood cells

[0121]

[0122] Table 2 Red blood cell abnormalities identified during red blood cell image recognition

[0123] Serial number abnormal 1 Anisocytosis 2 Macrocytic 3 Microcytic 4 Hypochromic red blood cells 5 Polychromatic red blood cells 6 Abnormal red blood cell shape 7 Red blood cell fragments 8 Acanthocytes 9 Bite mark red blood cells 10 vesicular red blood cells 11 Erythrocytes 12 Oval-shaped red blood cells 13 Irregularly contracted red blood cells 14 Oval red blood cells 15 sickle cell 16 Spherocytes 17 stomatocytes 18 Target cells 19 Teardrop-shaped red blood cells 20 Basophilic stippled red blood cells 21 Howe's body 22 Pappenheim's corpuscle 23 helmet-shaped red blood cells 24 parasites

[0124] Among the above-mentioned white blood cell abnormalities: the analysis mode corresponding to primitive cells, abnormal lymphocytes, nucleated red blood cells, and immature cells is the whole cell analysis mode, among which immature cells are the five types from "metamyelocytes" to "immature monocytes" in Table 1 above; the analysis mode corresponding to megakaryocytes among the white blood cell abnormalities is the abnormal white blood cell analysis mode; for other abnormalities among the white blood cell abnormalities, if the sample to be tested is a blood sample, the corresponding analysis mode is the blood white blood cell analysis mode; if the sample to be tested is a body fluid sample, the corresponding analysis mode is the body fluid white blood cell analysis mode; for all abnormalities among the above-mentioned red blood cell abnormalities, if the sample to be tested is a blood sample, the corresponding analysis mode is the blood red blood cell analysis mode; if the sample to be tested is a body fluid sample, the corresponding analysis mode is the body fluid red blood cell analysis mode; if platelet aggregation occurs in the blood sample, the corresponding analysis mode is the blood platelet aggregation analysis mode; if routine platelet abnormalities occur in the blood sample, the corresponding analysis mode is the blood platelet routine analysis mode. Of course, the analysis mode corresponding to the above-mentioned abnormalities can also be adjusted according to re-examination requirements, such as through user intervention to change the analysis mode corresponding to the abnormality.

[0125] In this embodiment, another optional structure of the imaging device 101 is Figure 13 and Figure 14 As shown, the cell image analysis device at least includes an imaging device 101 , a control device 102 and a smear moving device 103 .

[0126] The imaging device 101 includes a camera 1012 and a lens group 1011, which are used to photograph cells in the sample to be tested smeared on the sample smear. The smear moving device 103 is used to move the sample smear relative to the imaging device 101 so that the imaging device 101 can photograph the shooting area of ​​the sample smear to obtain a cell image. The control device 102 is used to identify and analyze the cell image of the sample smear.

[0127] like Figure 14 As shown, the lens assembly 1011 may include a first objective lens 10111 and a second objective lens 10112. One of the first objective lens 10111 and the second objective lens 10112 is a low-magnification objective lens, and the other is a high-magnification objective lens. For example, the first objective lens 10111 is a low-magnification objective lens, and the second objective lens 10112 is a high-magnification objective lens. For example, the first objective lens may be a 10x objective lens, and the second objective lens 10112 may be a 100x objective lens. The lens assembly 1011 may further include a third objective lens 10113. The magnification of the third objective lens 10113 is between the magnifications of the first objective lens and the second objective lens. For example, the third objective lens 10113 may be a 40x objective lens.

[0128] The cell image analysis device also includes an identification device 104, a smear clamping device 105, and a smear recovery device 106. The identification device 104 is used to identify the sample identification information of the sample smear. The smear clamping device 105 is used to clamp the sample smear from the identification device 104 to the smear moving device 103, so that the smear is driven by the smear moving device 103 to move relative to the imaging device 101. After the imaging device 101 captures the cell image of the sample smear, the control device 102 analyzes the cell image of the sample smear. The smear recovery device 106 is used to place the sample smear after the control device 102 completes the analysis.

[0129] The cell image analysis device also includes a smear basket loading device 107 for loading a smear basket containing sample smears to be tested. The smear clamping device 105 is also used to clamp the sample smears in the smear basket loaded on the smear basket loading device 107 to the identification device 104 for sample information identification.

[0130] For the above cell image analysis device, an optional structure of the control device in the cell image analysis device is as follows: Figure 15 As shown, it at least includes: a processing component 111, RAM112, ROM113, a communication interface 114, a memory 116 and an I / O interface 115, wherein the processing component 111, RAM112, ROM113, the communication interface 114, the memory 116 and the I / O interface 115 communicate through a bus 117.

[0131] The processing component may be a CPU, a GPU or other chips with computing capabilities.

[0132] The memory 116 contains various computer programs such as an operating system and application programs for the processor component 111 to execute, as well as data required to execute the computer programs. In addition, during the sample detection process, any information that needs to be stored locally can be stored in the memory 116.

[0133] The I / O interface 115 is comprised of a serial interface such as USB, IEEE1394, or RS-232C, a parallel interface such as SCSI, IDE, or IEEE1284, and an analog signal interface including a D / A converter and an A / D converter. Input devices such as a keyboard, mouse, touch screen, or other control buttons are connected to the I / O interface 115, allowing the user to directly input data into the control device 110. Furthermore, a display having a display function, such as an LCD screen, touch screen, or LED display, can also be connected to the I / O interface 115, allowing the control device to output information to the display in the form of an image display, such as analysis modes and cell images.

[0134] The communication interface 114 can be an interface that supports any currently known communication protocol. The communication interface 114 communicates with the outside world via a network. The control device can transmit data between any device connected to the network using a certain communication protocol via the communication interface 114.

[0135] See also Figure 16 , which shows an optional process of a sample analysis method provided in an embodiment of the present application, which may include the following steps:

[0136] S101: Controlling an imaging device to photograph a sample smear of a sample to be tested under a specified analysis mode to obtain a cell image of the sample to be tested, so that the control device can identify and analyze whether the sample smear has an abnormality corresponding to the specified analysis mode. The specified analysis mode at least indicates the shooting conditions when the imaging device 101 photographs the sample smear. For example, the shooting conditions are used to indicate at least one of the shooting position, shooting area, and number of images captured when photographing the sample smear, so as to ensure that the cell image captured by the imaging device 101 is consistent with the cell image required by the specified analysis mode.

[0137] Methods for determining the specified analysis mode include but are not limited to the following two methods:

[0138] One way is to determine the analysis mode selected by the user as the designated analysis mode, such as the user designating both the white blood cell analysis mode and the red blood cell analysis mode as the designated analysis mode, or the user designating only the white blood cell analysis mode as the designated mode.

[0139] Another way is: calling an analysis mode according to at least one of the detection result information of the sample to be tested and the sample information of the sample to be tested, and determining the called analysis mode as the designated analysis mode.

[0140] S102: performing identification analysis on the cell image of the sample to be tested captured by the imaging device to identify whether other abnormalities exist in the sample smear.

[0141] S103: When it is identified that other abnormalities exist in the sample smear that are different from the abnormalities corresponding to the designated analysis mode, the analysis modes corresponding to the other abnormalities are used as additional analysis modes.

[0142] The abnormality corresponding to the designated analysis mode and the other abnormality are two different types of abnormalities and can be directed to different analysis modes. These two different types of abnormalities can be abnormalities for different cell types. For example, if the abnormality corresponding to the designated analysis mode is for white blood cells, and the other abnormality is for red blood cells, in this embodiment, the analysis mode corresponding to the other abnormality can be selected as the additional analysis mode.

[0143] The abnormalities corresponding to the above-mentioned designated analysis mode and other abnormalities may be abnormalities for the same type of cells but can correspond to different analysis modes. For example, the abnormality corresponding to the designated analysis mode is for white blood cells, and the other abnormalities are also for white blood cells, but the analysis mode corresponding to the other abnormalities is not the designated analysis mode.

[0144] In this embodiment, the control method of the imaging device in the specified analysis mode and the additional analysis mode can be: after the imaging device completes the sample smear shooting in the specified analysis mode, it continues to call the additional analysis mode to shoot the sample smear, that is, the imaging device will shoot in the specified analysis mode and the additional analysis mode in sequence, so that multiple abnormalities in the sample to be tested can be re-examined in one re-examination, thereby improving the processing effect and the accuracy of the processing results.

[0145] S104: Controlling the imaging device to photograph the sample smear in the additional analysis mode, so as to further perform identification analysis on the photographed cell images.

[0146] As can be seen from the description of the cell image analysis device described above, the imaging device captures a sample smear of the sample to be tested under a specified analysis mode to obtain a cell image of the sample to be tested, and then identifies and analyzes the cell image of the sample to be tested. When an abnormality other than the abnormality corresponding to the specified analysis mode is identified in the sample smear, the analysis mode corresponding to the other abnormality is used as an additional analysis mode, and the imaging device is controlled to capture the sample smear under the additional analysis mode to further identify and analyze the captured cell image. This allows the imaging device to automatically capture and analyze the cell image of the sample smear if a different abnormality is found when processing the sample smear under the specified analysis mode, thereby automatically adding and calling the additional analysis mode. The additional analysis mode matches the other abnormality found, allowing the imaging device to capture cell images that match the other abnormality under the guidance of the additional analysis mode to identify and analyze the cell images that match the other abnormality, thereby improving processing efficiency and the accuracy of processing results.

[0147] In this embodiment, the designated analysis mode and the additional analysis mode may include one or more analysis modes. The designated analysis mode and the additional analysis mode are expressed as follows:

[0148] The designated analysis modes include: a blood white blood cell analysis mode for photographing and analyzing white blood cells in a blood sample smear, a blood red blood cell analysis mode for photographing and analyzing red blood cells in a blood sample smear, a blood platelet routine analysis mode for photographing and analyzing platelets in a blood sample smear, a blood platelet aggregation analysis mode for photographing platelets in a blood sample smear to identify and analyze their aggregation status, an abnormal white blood cell analysis mode for photographing white blood cells in a blood sample smear to identify and analyze giant abnormal cells, a body fluid white blood cell analysis mode for photographing and analyzing white blood cells in a body fluid sample smear, a body fluid red blood cell analysis mode for photographing and analyzing red blood cells in a body fluid sample smear, and at least one of the following analysis modes: a whole cell analysis mode for photographing and analyzing all cells in a designated area of ​​a sample smear.

[0149] The additional analysis mode is one or more of: blood white blood cell analysis mode, blood red blood cell analysis mode, blood platelet routine analysis mode, body fluid white blood cell analysis mode, body fluid red blood cell analysis mode, whole cell analysis mode, abnormal white blood cell analysis mode and platelet aggregation analysis mode. The additional analysis mode is different from the specified analysis mode and is an analysis mode corresponding to other abnormalities.

[0150] If the designated analysis mode is the conventional blood and platelet analysis mode, the process of controlling the imaging device to photograph the sample smear of the sample to be tested in the designated analysis mode includes: if the designated analysis mode is the conventional blood and platelet analysis mode, the shooting area is determined by searching through the low-magnification objective lens in the imaging device, and the platelets in the shooting area are photographed through the high-magnification objective lens in the imaging device. The shooting area is the junction of the body and tail of the sample smear. Please refer to the above description for details.

[0151] A blood platelet aggregation analysis mode and an abnormal white blood cell analysis mode are introduced into the designated analysis mode and the supplementary analysis mode. This allows direct selection of at least one of the two analysis modes within the designated analysis mode. Alternatively, if platelet abnormalities are detected in a cell image analyzed under the designated analysis mode, the blood platelet aggregation analysis mode can be used as the supplementary analysis mode. Specifically, if platelet abnormalities are present in a smear of a sample indicating other abnormalities, the blood platelet positive analysis mode can be used as the supplementary analysis mode. Furthermore, if at least one of giant abnormal white blood cells, such as megakaryocytes and giant immature cells, is detected under the designated analysis mode, the abnormal white blood cell analysis mode can be used as the supplementary analysis mode. Specifically, if giant abnormal white blood cells are present in a smear of a sample indicating other abnormalities than those corresponding to the designated analysis mode, the abnormal white blood cell analysis mode can be used as the supplementary analysis mode.

[0152] Among them, platelet abnormality includes at least one of platelet aggregation and low platelet value (such as the platelet count value is lower than the normal range). Platelet aggregation is used to indicate that platelets in the sample to be tested have aggregated (such as concentrated in a certain area). For platelet abnormality, it is necessary to guide the imaging device to shoot through the blood platelet aggregation analysis mode to identify and analyze whether there is really severe platelet aggregation, so as to determine whether the platelet abnormality is caused by platelet aggregation, especially whether the low platelet value is caused by platelet aggregation, so as to determine whether the low platelet value is a false decrease.

[0153] Compared to the designated analysis mode, the analysis requirements in the additional analysis mode may be different from the analysis requirements in the designated analysis mode, and the shooting area in the additional analysis mode may also be different from the shooting area in the designated analysis mode. Based on this, the sample analysis method provided in this embodiment may further include the following steps:

[0154] A second photographing area different from the first photographing area in the additional analysis mode is determined, and the imaging device is instructed to photograph the second photographing area in the sample smear, wherein the first photographing area is the photographing area in the sample smear in the specified analysis mode.

[0155] The imaging device is controlled to photograph the second photographing area in the sample smear in the additional analysis mode to obtain a cell image of the second photographing area.

[0156] The cell image of the second photographed area is analyzed.

[0157] The first shooting area and the second shooting area are two different shooting areas in the sample smear, and the difference is reflected in at least one of: the first shooting area and the second shooting area are two completely independent shooting areas, one of the first shooting area and the second shooting area is larger than the other shooting area, and parts of the first shooting area and the second shooting area overlap.

[0158] In this embodiment, the first shooting area and the second shooting area may be set in a manner that: at least one of the first shooting area and the second shooting area is set in advance, such as the first shooting area is set in advance by a user, and the second shooting area is obtained based on the first shooting area. One manner of obtaining the second shooting area based on the first shooting area is that: the second shooting area is extended from the first shooting area in a specified direction, and the specified direction can indicate that the imaging device captures a cell image that matches other abnormalities, that is, the captured cell image may indicate other abnormalities. For example, when the other abnormality is a platelet-related abnormality, it is necessary to capture the tail and / or edge of the sample smear, and then the specified direction is the tail and / or edge.

[0159] The first imaging area is the junction of the body and tail of the sample smear, and the second imaging area may include at least one of the tail and edge of the sample smear. Alternatively, the second imaging area is extended from the body and tail junction toward at least one of the tail and edge of the sample smear in a specified direction, so that at least one of the areas extending to or near the tail of the sample smear and the edge corresponding to the body and tail junction serves as the second imaging area, enabling the imaging device to image the tail of the sample smear and / or the edge corresponding to the body and tail junction. The reason for extending toward at least one of the specified directions of the tail and the edge is to determine, by capturing cell images at the tail and / or edge of the sample smear, whether an abnormality in the sample to be tested is caused by the concentration of cells in the sample to the tail and / or edge. If the cells are concentrated at the tail and / or edge, other abnormalities can be reviewed based on the concentration of cells at the tail and / or edge.

[0160] In this embodiment, the second shooting area may also be set in other ways, such as by user selection. The corresponding sample analysis method may further include the following steps:

[0161] A sample smear is shown.

[0162] The photographing area selected by the user in the displayed sample smear is acquired, and the selected photographing area is used as the second photographing area.

[0163] The selected shooting area is at least one of the tail of the sample smear and the edge of the sample smear. The edge of the sample smear may be an edge corresponding to the junction of the body and the tail of the sample smear.

[0164] The selection of the additional analysis mode is described below. In this embodiment, the additional analysis mode can be selected in at least one of the following ways:

[0165] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the blood leukocyte analysis mode, the blood leukocyte analysis mode is the analysis mode corresponding to the other abnormalities.

[0166] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the red blood cell analysis mode, the red blood cell analysis mode is the analysis mode corresponding to the other abnormalities.

[0167] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the blood and platelet routine analysis mode, the blood and platelet routine analysis mode is the analysis mode corresponding to the other abnormalities.

[0168] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the blood platelet aggregation analysis mode, the blood platelet aggregation analysis mode is the analysis mode corresponding to the other abnormalities.

[0169] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the whole-cell analysis mode, the whole-cell analysis mode is the analysis mode corresponding to the other abnormalities;

[0170] If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the abnormal white blood cell analysis mode, the abnormal white blood cell analysis mode is the analysis mode corresponding to the other abnormalities.

[0171] If the sample to be tested is a body fluid sample and the other abnormalities correspond to abnormalities in the body fluid leukocyte analysis mode, the body fluid leukocyte analysis mode is the analysis mode corresponding to the other abnormalities.

[0172] If the sample to be tested is a body fluid sample and the other abnormalities correspond to abnormalities in the body fluid red blood cell analysis mode, the body fluid red blood cell analysis mode is the analysis mode corresponding to the other abnormalities.

[0173] If the sample to be tested is a body fluid sample and the other abnormalities correspond to abnormalities in the whole-cell analysis mode, the whole-cell analysis mode is the analysis mode corresponding to the other abnormalities.

[0174] By selecting the additional analysis mode, a matching analysis mode can be determined for abnormalities of any type of cells in blood samples and body fluid samples, thereby improving processing efficiency and accuracy.

[0175] In this embodiment, the sample analysis method may further include the following steps:

[0176] The cell images captured in the specified analysis mode and the cell images captured in the additional analysis mode are displayed in different display modes; and / or the identification analysis results corresponding to the specified analysis mode and the identification analysis results corresponding to the additional analysis mode are displayed in different display modes. For detailed description, please refer to the above description, which will not be elaborated in this embodiment.

[0177] An embodiment of the present application further provides a storage medium storing executable instructions, which is configured to cause a processor to execute the executable instructions to implement the above-mentioned sample analysis method.

[0178] Those skilled in the art will appreciate that embodiments of the present invention may be provided as methods, systems, or computer program products. Therefore, embodiments of the present invention may take the form of hardware embodiments, software embodiments, or embodiments combining software and hardware. Furthermore, embodiments of the present invention may take the form of a computer program product implemented on one or more computer-usable storage media (including magnetic disk storage and optical storage, etc.) containing computer-usable program code.

[0179] The embodiments of the present invention are described with reference to the flowcharts and / or block diagrams of the methods, devices (systems), and computer program products according to the embodiments of the present invention. It should be understood that each process and / or block in the flowchart and / or block diagram, as well as the combination of the processes and / or blocks in the flowchart and / or block diagram, can be implemented by computer program operations. These computer programs can be provided to a processor of a general-purpose computer, a special-purpose computer, an embedded processor, or other programmable data processing device to produce a machine, so that the operations performed by the processor of the computer or other programmable data processing device produce the operations for implementing the processes in the flowchart and / or block diagram. Figure 1 a process or multiple processes and / or boxes Figure 1 A device that provides the functions specified in a block or multiple blocks.

[0180] These computer program operations may also be stored in a computer readable memory that can direct a computer or other programmable data processing device to operate in a specific manner, so that the operations stored in the computer readable memory produce an article of manufacture including an operating device that implements the process Figure 1 a process or multiple processes and / or boxes Figure 1 The function specified in one or more boxes.

[0181] These computer program operations can also be loaded onto a computer or other programmable data processing device so that a series of operational steps are executed on the computer or other programmable device to produce a computer-implemented process, thereby providing the operations executed on the computer or other programmable device for implementing the process. Figure 1 a process or multiple processes and / or boxes Figure 1 A step that specifies a function in one or more boxes.

[0182] The above description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A cell image analysis device, comprising: An imaging device configured to photograph a sample smear of a sample to be tested in a specified analysis mode to obtain a cell image of the sample to be tested, so that the control device can identify and analyze whether the sample smear has an abnormality corresponding to the specified analysis mode; The controls are configured for: Performing identification and analysis on the cell image of the sample to be tested taken by the imaging device to identify whether there are other abnormalities in the sample smear that are different from the abnormalities corresponding to the specified analysis mode, wherein the other abnormalities are abnormalities of cells in the cell image, including: identifying the cell type belonging to the abnormal cell from the cell image or identifying the abnormal condition of the cell from the cell image; When it is identified that there are other abnormalities in the sample smear that are different from the abnormalities corresponding to the specified analysis mode, the analysis mode corresponding to the other abnormalities is used as an additional analysis mode; the specified analysis mode and the additional analysis mode at least indicate the shooting conditions when the imaging device shoots the sample smear, and the shooting conditions include at least one of a shooting position, a shooting area, and a number of image shootings; The imaging device is controlled to photograph the sample smear in the additional analysis mode to obtain the other abnormally matched cell images, so as to further identify and analyze the photographed other abnormally matched cell images.

2. The device according to claim 1, wherein: The control device is configured to enable the imaging device to continue to call the additional analysis mode to photograph the sample smear after completing the photographing of the sample smear in the designated analysis mode.

3. The device according to claim 1, wherein: The control device is configured to determine the analysis mode selected by the user as the designated analysis mode.

4. The device according to claim 1, wherein: The control device is configured to call the analysis mode according to at least one of the detection result information of the sample to be tested and the sample information of the sample to be tested, and determine the called analysis mode as the designated analysis mode.

5. The device according to claim 1, wherein: The designated analysis modes include: a blood white blood cell analysis mode for photographing and analyzing white blood cells in a blood sample smear, a blood red blood cell analysis mode for photographing and analyzing red blood cells in a blood sample smear, a blood platelet routine analysis mode for photographing and analyzing platelets in a blood sample smear, a blood platelet aggregation analysis mode for photographing platelets in a blood sample smear to identify and analyze their aggregation conditions, an abnormal white blood cell analysis mode for photographing white blood cells in a blood sample smear to identify and analyze giant abnormal cells, a body fluid white blood cell analysis mode for photographing and analyzing white blood cells in a body fluid sample smear, a body fluid red blood cell analysis mode for photographing and analyzing red blood cells in a body fluid sample smear, and at least one of the following analysis modes: a whole cell analysis mode for photographing and analyzing all cells in a designated area of ​​a sample smear.

6. The device according to claim 5, wherein: The additional analysis mode is one or more of: blood white blood cell analysis mode, blood red blood cell analysis mode, blood platelet routine analysis mode, body fluid white blood cell analysis mode, body fluid red blood cell analysis mode, whole cell analysis mode, abnormal white blood cell analysis mode and blood platelet aggregation analysis mode. The additional analysis mode is different from the designated analysis mode and is an analysis mode corresponding to the other abnormalities.

7. The device according to claim 6, wherein: If the designated analysis mode is the conventional blood and platelet analysis mode, the imaging device is further configured to photograph the sample smear of the sample to be tested in the designated analysis mode to obtain a cell image of the sample to be tested: in the conventional blood and platelet analysis mode, a shooting area is determined by searching with a low-power objective lens in the imaging device, and the platelets in the shooting area are photographed with a high-power objective lens in the imaging device, and the shooting area is the junction of the body and tail of the sample smear.

8. The device according to claim 1, wherein: The control device is further configured to, when it is identified that other abnormalities different from the abnormality corresponding to the specified analysis mode exist in the sample smear, use the analysis mode corresponding to the other abnormality as the additional analysis mode: If the other abnormality indicates the presence of platelet abnormality in the sample smear, the blood platelet aggregation analysis mode is used as the additional analysis mode.

9. The device according to claim 1, wherein: The control device is further configured to, when it is identified that other abnormalities different from the abnormality corresponding to the specified analysis mode exist in the sample smear, use the analysis mode corresponding to the other abnormality as the additional analysis mode: If the other abnormality indicates the presence of macro-abnormal leukocytes in the sample smear, the abnormal leukocyte analysis mode is used as the additional analysis mode.

10. The device according to claim 1, wherein: The control device is further configured to determine a second photographing area different from the first photographing area in the additional analysis mode, and instruct the imaging device to photograph the second photographing area in the sample smear, the first photographing area being the photographing area in the sample smear in the specified analysis mode; The imaging device is further configured to photograph a sample smear of the sample to be tested in a specified analysis mode to obtain a cell image of the sample to be tested: photograph a second photographing area in the sample smear in the additional analysis mode to obtain a cell image of the second photographing area; The control device is further configured to: when performing identification and analysis on the captured cell image: perform identification and analysis on the cell image of the second captured area.

11. The device according to claim 10, wherein: The first shooting area is the junction of the body and tail of the sample smear, and the second shooting area includes at least one of the tail and edge of the sample smear.

12. The device according to claim 1, wherein: The device further comprises: a display device, the display device being used to display the sample smear; The control device is further configured to obtain a shooting area selected by a user in the displayed sample smear, and use the selected shooting area as the second shooting area.

13. The device according to claim 12, wherein: The selected photographing area is at least one of a tail of the sample smear and an edge of the sample smear.

14. The device according to any one of claims 1 to 13, wherein: The control device is further configured to, when identifying that there are other abnormalities in the sample smear that are different from the abnormality corresponding to the specified analysis mode, use the analysis mode corresponding to the other abnormality as the additional analysis mode in at least one of the following ways: If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in a blood leukocyte analysis mode, the blood leukocyte analysis mode is an analysis mode corresponding to the other abnormality; If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in a blood red blood cell analysis mode, the blood red blood cell analysis mode is an analysis mode corresponding to the other abnormality; If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in the blood platelet routine analysis mode, the blood platelet routine analysis mode is the analysis mode corresponding to the other abnormality; If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in the blood platelet aggregation analysis mode, the blood platelet aggregation analysis mode is the analysis mode corresponding to the other abnormality; If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in the whole-cell analysis mode, the whole-cell analysis mode is the analysis mode corresponding to the other abnormality; If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the abnormal white blood cell analysis mode, the abnormal white blood cell analysis mode is the analysis mode corresponding to the other abnormalities.

15. The device according to any one of claims 1 to 13, wherein: The control device is further configured to, when identifying that there are other abnormalities in the sample smear that are different from the abnormality corresponding to the specified analysis mode, use the analysis mode corresponding to the other abnormality as the additional analysis mode in at least one of the following ways: If the sample to be tested is a body fluid sample and the other abnormality corresponds to an abnormality in the body fluid leukocyte analysis mode, the body fluid leukocyte analysis mode is the analysis mode corresponding to the other abnormality; If the sample to be tested is a body fluid sample and the other abnormality corresponds to an abnormality in the body fluid red blood cell analysis mode, the body fluid red blood cell analysis mode is the analysis mode corresponding to the other abnormality; If the sample to be tested is a body fluid sample and the other abnormality corresponds to an abnormality in the whole-cell analysis mode, the whole-cell analysis mode is the analysis mode corresponding to the other abnormality.

16. The device according to any one of claims 1 to 13, wherein: The cell image captured in the designated analysis mode and the cell image captured in the additional analysis mode are displayed differently; and / or The recognition analysis result corresponding to the designated analysis mode and the recognition analysis result corresponding to the additional analysis mode are displayed in different ways.

17. The device according to any one of claims 1 to 13, wherein: The abnormal conditions of cells identified from the cell images include at least one of the following: abnormal conditions of white blood cells, abnormal conditions of red blood cells, and abnormal conditions of platelets; the abnormal conditions of platelets include at least one of platelet aggregation and low platelet count.

18. A sample analysis method, comprising: Controlling the imaging device to photograph the sample smear of the sample to be tested in a specified analysis mode to obtain a cell image of the sample to be tested, so that the control device can identify and analyze whether the sample smear has an abnormality corresponding to the specified analysis mode; Performing identification and analysis on the cell image of the sample to be tested obtained by the imaging device to identify whether there are other abnormalities in the sample smear that are different from the abnormalities corresponding to the specified analysis mode, wherein the other abnormalities are abnormalities of cells in the cell image, including: identifying the cell type belonging to the abnormal cell from the cell image or identifying the abnormal condition of the cell from the cell image; When it is identified that there are other abnormalities in the sample smear that are different from the abnormalities corresponding to the specified analysis mode, the analysis mode corresponding to the other abnormalities is used as an additional analysis mode; the specified analysis mode and the additional analysis mode at least indicate the shooting conditions when the imaging device shoots the sample smear, and the shooting conditions include at least one of a shooting position, a shooting area, and a number of image shootings; The imaging device is controlled to photograph the sample smear in the additional analysis mode to obtain the other abnormally matched cell images, so as to further identify and analyze the photographed other abnormally matched cell images.

19. The method according to claim 18, wherein: The controlling the imaging device to photograph the sample smear in the additional analysis mode comprises: After the imaging device completes photographing the sample smear in the designated analysis mode, the additional analysis mode is continuously called to photograph the sample smear.

20. The method according to claim 18, wherein: The designated analysis modes include: a blood white blood cell analysis mode for photographing and analyzing white blood cells in a blood sample smear, a blood red blood cell analysis mode for photographing and analyzing red blood cells in a blood sample smear, a blood platelet routine analysis mode for photographing and analyzing platelets in a blood sample smear, a blood platelet aggregation analysis mode for photographing platelets in a blood sample smear to identify and analyze their aggregation conditions, an abnormal white blood cell analysis mode for photographing white blood cells in a blood sample smear to identify and analyze giant abnormal cells, a body fluid white blood cell analysis mode for photographing and analyzing white blood cells in a body fluid sample smear, a body fluid red blood cell analysis mode for photographing and analyzing red blood cells in a body fluid sample smear, and at least one of a whole cell analysis mode for photographing and analyzing all cells in a designated area of ​​a sample smear; The additional analysis mode is one or more of: blood white blood cell analysis mode, blood red blood cell analysis mode, blood platelet routine analysis mode, body fluid white blood cell analysis mode, body fluid red blood cell analysis mode, whole cell analysis mode, abnormal white blood cell analysis mode and platelet aggregation analysis mode. The additional analysis mode is different from the designated analysis mode and is an analysis mode corresponding to the other abnormalities.

21. The method according to claim 20, wherein: The controlling the imaging device to photograph the sample smear of the sample to be tested in the designated analysis mode comprises: if the designated analysis mode is the conventional blood platelet analysis mode, searching and determining a photographing area through a low-power objective lens in the imaging device, photographing the platelets in the photographing area through a high-power objective lens in the imaging device, and the photographing area is the junction of the body and tail of the sample smear.

22. The method according to any one of claims 18, wherein: When it is identified that there are other abnormalities in the sample smear that are different from the abnormality corresponding to the specified analysis mode, the analysis mode corresponding to the other abnormality is used as an additional analysis mode, which includes: If the other abnormality indicates the presence of platelet abnormality in the sample smear, the blood platelet aggregation analysis mode is used as the additional analysis mode.

23. The method according to any one of claims 18, wherein: When it is identified that there are other abnormalities in the sample smear that are different from the abnormality corresponding to the specified analysis mode, the analysis mode corresponding to the other abnormality is used as an additional analysis mode, which includes: If the other abnormality indicates the presence of macro-abnormal leukocytes in the sample smear, the abnormal leukocyte analysis mode is used as the additional analysis mode.

24. The method according to claim 18, wherein: The method further includes: determining a second photographing area different from the first photographing area in the additional analysis mode, and instructing the imaging device to photograph the second photographing area in the sample smear, the first photographing area being the photographing area in the sample smear in the specified analysis mode; Controlling the imaging device to photograph a second photographing area in the sample smear in the additional analysis mode to obtain a cell image of the second photographing area; The cell image of the second photographing area is analyzed.

25. The method according to claim 24, wherein: The first shooting area is the junction of the body and tail of the sample smear, and the second shooting area includes at least one of the tail and edge of the sample smear.

26. The method according to any one of claims 18 to 25, wherein: When it is identified that there are other abnormalities in the sample smear that are different from the abnormality corresponding to the specified analysis mode, at least one of the following methods is used to use the analysis mode corresponding to the other abnormality as an additional analysis mode: If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in a blood leukocyte analysis mode, the blood leukocyte analysis mode is an analysis mode corresponding to the other abnormality; If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in a blood red blood cell analysis mode, the blood red blood cell analysis mode is an analysis mode corresponding to the other abnormality; If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in the blood platelet routine analysis mode, the blood platelet routine analysis mode is the analysis mode corresponding to the other abnormality; If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in the blood platelet aggregation analysis mode, the blood platelet aggregation analysis mode is the analysis mode corresponding to the other abnormality; If the sample to be tested is a blood sample and the other abnormality corresponds to an abnormality in the whole-cell analysis mode, the whole-cell analysis mode is the analysis mode corresponding to the other abnormality; If the sample to be tested is a blood sample and the other abnormalities correspond to abnormalities in the abnormal white blood cell analysis mode, the abnormal white blood cell analysis mode is the analysis mode corresponding to the other abnormalities.

27. The method according to any one of claims 18 to 25, wherein: When it is identified that there are other abnormalities in the sample smear that are different from the abnormality corresponding to the specified analysis mode, at least one of the following methods is used to use the analysis mode corresponding to the other abnormality as an additional analysis mode: If the sample to be tested is a body fluid sample and the other abnormality corresponds to an abnormality in the body fluid leukocyte analysis mode, the body fluid leukocyte analysis mode is the analysis mode corresponding to the other abnormality; If the sample to be tested is a body fluid sample and the other abnormality corresponds to an abnormality in the body fluid red blood cell analysis mode, the body fluid red blood cell analysis mode is the analysis mode corresponding to the other abnormality; If the sample to be tested is a body fluid sample and the other abnormality corresponds to an abnormality in the whole-cell analysis mode, the whole-cell analysis mode is the analysis mode corresponding to the other abnormality.

28. The method according to any one of claims 18 to 25, wherein: The method further comprises: Displaying the cell image captured in the designated analysis mode and the cell image captured in the additional analysis mode in different display modes; and / or The recognition analysis result corresponding to the designated analysis mode and the recognition analysis result corresponding to the additional analysis mode are displayed in different display modes.

29. The method according to any one of claims 18 to 25, wherein: The abnormal conditions of cells identified from the cell images include at least one of the following: abnormal conditions of white blood cells, abnormal conditions of red blood cells, and abnormal conditions of platelets; the abnormal conditions of platelets include at least one of platelet aggregation and low platelet count.

30. A storage medium having executable instructions stored thereon, configured to cause a processor to execute the executable instructions to implement the sample analysis method according to any one of claims 18 to 29.

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