A hair restorer containing stem cell extract and a method of using the same

Abstract

The application discloses a hair restorer containing stem cell extract and a use method thereof. 100 mL of the hair restorer comprises the following raw materials: stem cell extract freeze-dried powder 50-250 mg, beta nicotinamide mononucleotide 100-650 mg, coenzyme Q10 10-120 mg, melatonin 10-220 mg, ginsenoside A2 10-240 mg, chuanxiong extract 0.2-2 g, safflower extract 0.2-2 g, dendrobium extract 0.3-3 g, radix falcaliae extract 1-8 g, ligustrum lucidum extract 1-8 g, and the rest is deionized water. The hair restorer contains stem cell growth factors, NMN metabolic energy substances, and is supplemented with coenzyme Q10, melatonin, ginsenoside A2, chuanxiong extract, safflower extract, dendrobium extract, radix falcaliae extract and ligustrum lucidum extract, and can promote hair follicle cell growth and hair growth.

Description

TECHNICAL FIELD

[0001] The present application relates to a hair restorer containing stem cell extract and a method of use thereof, and belongs to the technical field of hair care. BACKGROUND

[0002] With the acceleration of the pace of life, the increasing work pressure, more and more people are worried about hair loss. There are various types of hair loss, many types, different situations, the common ones are alopecia areata, alopecia totalis and dandruff alopecia. Alopecia areata is a localized patchy hair loss, characterized by normal skin without inflammation and no symptoms in the affected area, often discovered accidentally. There are round or oval hair loss patches on the head, the hair on the edge of the bald area is loose and easy to pull out. The course of alopecia areata can last for several months to several years, and most of them can recover on their own, but some have recurrent attacks. Alopecia totalis often occurs in young men and is more common in brain workers. The hair is dry and lacks luster. Dandruff alopecia is also common in young men. The scalp is often oily and shiny or has a large amount of dandruff, which is gray and scurfy, and itchy. Over time, the hair on both sides of the forehead and the top of the head begins to fall out symmetrically, becoming sparse and thin. The affected area often leads to permanent hair loss due to atrophy of the hair follicles. Since hair plays an important role in appearance and is also an indicator of physical health, hair restorers have received increasing attention and attention from more and more consumers. Current hair restorers can be divided into chemical hair restorers and Chinese herbal hair restorers. The safety of chemical hair restorers is questionable, and their effects are single. Chinese herbal hair restorers are of various types and have different effects, and suitable Chinese herbal ingredients still need to be screened. Therefore, a hair restorer containing stem cell extract is necessary. SUMMARY

[0003] In order to overcome the shortcomings of the prior art, the present application provides a hair restorer containing stem cell extract and a method of use thereof.

[0004] The present application is realized by the following technical solutions:

[0005] A hair restorer containing stem cell extract, 100mL of the hair restorer comprises the following raw materials: stem cell extract freeze-dried powder 50-250mg, beta nicotinamide mononucleotide (NMN) 100-650mg, coenzyme Q10 10-120mg, melatonin 10-220mg, ginsenoside A2 10-240mg, chuanxiong extract 0.2-2g, safflower extract 0.2-2g, dendrobium extract 0.3-3g, radix falcaliae extract 1-8g, ligustrum lucidum extract 1-8g, and the rest is deionized water.

[0006] The hair growth agent containing stem cell extract, 100ml of the hair growth agent comprises the following raw materials: stem cell extract freeze-dried powder 200mg, beta nicotinamide mononucleotide 600mg, coenzyme Q10 100mg, melatonin 200mg, ginsenoside A2 200mg, chuanqiong extract 1.5g, safflower extract 1.5g, dendrobium extract 2g, radix falcaliae extract 6g, ligustrum lucidum extract 5g, and the rest is deionized water.

[0007] The hair growth agent containing stem cell extract, the stem cell extract refers to the extract of the jellyfish medusa stem cell.

[0008] The hair growth agent containing stem cell extract, the extract of the jellyfish medusa stem cell is obtained by the following steps:

[0009] (1) Cleanly feed the living medusa for 24-48 hours, repeatedly rinse with sterile normal saline, disinfect with 70% alcohol, remove the skin and tentacles under sterile conditions at 4℃, crush and filter, add cell separation digestion solution and buffer culture solution to prepare a single cell suspension, centrifugally wash with normal saline for 3 times, add an appropriate amount of culture medium, incubate in a 37℃ carbon dioxide incubator for 45 minutes, and then incubate overnight in an ice bath at 4℃;

[0010] (2) Use a flow cytometer to separate stem cells from the cell suspension with stem cell markers, collect the stem cells, centrifugally wash with sterile conditions at 4℃ for 3 times, remove the markers, and add buffer solution to prepare a cell suspension;

[0011] (3) Add the cell suspension to pre-cooled buffer solution, ultrasonically break the cells under sterile conditions, centrifuge at 4℃, collect the supernatant, measure the protein concentration, and prepare a stem cell extract containing 1-2×10 8 cells per ml;

[0012] (4) Mix the obtained stem cell extract with albumin, normal saline mixture, and excipients uniformly to obtain the hair growth agent containing stem cell extract.

[0013] The hair growth agent containing stem cell extract, the chuanqiong extract is obtained by the following steps: place chuanqiong in water, reflux extract under the condition that the temperature is 90-100℃, obtain water extract, extract twice, combine and concentrate the water extract of two times, dry to obtain paste-like chuanqiong extract.

[0014] The hair growth agent containing stem cell extract, the safflower extract is obtained by the following steps: after safflower is crushed, 70-80v / v% ethanol aqueous solution is used to extract at the temperature of 30-40℃, and the ethanol extract is obtained; the ethanol extract is concentrated, and then passed through a macroporous resin, 80-90% ethanol aqueous solution is used for elution, and the safflower extract is obtained by freeze-drying after concentration.

[0015] The hair growth agent containing stem cell extract, the dendrobium extract is obtained by the following steps: after dendrobium is crushed, it is placed in water and extracted at the temperature of 90-100℃, and the water extract is obtained; the water extract is concentrated, 95v / v% ethanol aqueous solution is added to dissolve, and the dendrobium extract is obtained by freeze-drying after concentration.

[0016] The hair growth agent containing stem cell extract, the radix polygoni multiflori extract is obtained by the following steps: after radix polygoni multiflori is placed in water and extracted at the temperature of 90-100℃, the extract is obtained; the extract is concentrated, ethanol is added, and the alcohol precipitate is collected after standing for 24-48h; and the radix polygoni multiflori extract is obtained by freeze-drying the alcohol precipitate.

[0017] The hair growth agent containing stem cell extract, the ligustrum lucidum extract is obtained by the following steps: after ligustrum lucidum is crushed, 70-80v / v% ethanol is used to reflux extract at the temperature of 60-70℃, and the ethanol extract is obtained; the ethanol extract is concentrated, and then passed through a macroporous resin, 80-90%v / v ethanol aqueous solution is used for elution, and the ligustrum lucidum extract is obtained by drying after concentration.

[0018] The use method of the hair growth agent containing stem cell extract, the hair growth agent is sterilized by a 0.22μm sterile filter, and the color is removed by ultrafiltration, and then the hair growth agent is applied to the scalp of the alopecia area or used for shampooing.

[0019] The stem cell is a kind of cell with regeneration and self-renewal, that is, a kind of cell with proliferation, self-repair, mass production and differentiation into offspring. It is of great significance for the repair of necrotic or pathological tissue of human body and the replacement of aging apoptotic cells. However, the culture technology of human stem cells is complex and expensive, and the cost of a treatment is tens of thousands of yuan, but the stem cells of animals and plants, especially the Aequorea victoria stem cell, can be cultured in seawater, which is low in cost and can be cultured in large quantities, so it can be widely used, and the benefits of stem cells can be brought to ordinary people.

[0020] Nicotinamide mononucleotide is an important substance in human cell energy generation, which is involved in the synthesis of NAD (nicotinamide adenine dinucleotide, an important coenzyme for cell energy conversion) in cells. Nicotinamide mononucleotide decreases significantly with age. Studies have shown that nicotinamide mononucleotide can increase NAD in various organs of experimental rats, improve metabolism, increase energy supply, thereby inhibiting the aging of neurovascular and systemic tissues and organs, and also improving the symptoms of diabetes. In particular, it can prevent the expression of age-related aging genes in a tissue-specific manner, enhance mitochondrial oxidative metabolism of skeletal muscle, and achieve anti-aging effects. Recent studies have found that adjusting the level of NMN in the body has good treatment and repair effects on cardiovascular and cerebrovascular diseases, neurodegenerative diseases, and aging degenerative diseases. NMN can be absorbed through essence, significantly improving skin metabolism and increasing energy supply, reducing pigment deposition, whitening, and making the skin look vigorous; it can also resist oxidation and prevent aging, reduce and relieve skin wrinkles, protect DNA, and maintain a youthful state of the skin.

[0021] In addition to improving metabolism, coenzyme Q10 has strong antioxidant effects, scavenges free radicals, protects cells from damage, and protects cell health. The aging of the skin and the increase in wrinkles are also related to the content of Q10. The lower the content, the more likely the skin is to age, and the more wrinkles there are on the face. Coenzyme Q10 can increase the concentration of hyaluronic acid in the skin, improve skin hydration, and have good effects on improving dull skin, reducing wrinkles, maintaining smooth, elastic, and moist skin, thereby enhancing keratinocyte activity, reducing cell apoptosis, and making you younger.

[0022] Melatonin (MT) is one of the hormones secreted by the pineal gland. MT has strong neuroendocrine immune regulation activity and free radical scavenging antioxidant capacity. The basic function of melatonin is to participate in the antioxidant system to prevent oxidative damage to cells and can also regulate the activity of various cytokines. It has the functions of whitening and removing spots, regulating cardiovascular function, and preventing the occurrence of tumors. In addition, it can also improve immunity, improve sleep quality, and restore sub-health.

[0023] Ginsenoside A2 has the effects of benefiting qi and strengthening yang, enhancing cell vitality, promoting stem cell proliferation, enhancing metabolism, improving immunity, restoring sub-health, and resisting aging.

[0024] Polygonum multiflorum extract has anti-aging and immune-regulating effects, and also has the effects of tonifying liver and kidney, and tonifying kidney and replenishing qi to promote hair growth.

[0025] Female ginseng is flat, sweet and bitter in taste, and belongs to liver and kidney channels, with the effects of nourishing yin and longevity, tonifying liver and kidney, clearing heat and improving eyesight, blackening beard and hair, and improving metabolism and hair growth.

[0026] Chuanxiong is pungent and warm, and has the function of dispersing and descending. It can reach the top of the head and the blood. It has the function of activating blood and removing blood stasis, and is suitable for various diseases caused by blood stasis. It can treat headache, rheumatism and other symptoms

[0027] Safflower is a medicine for blood. Safflower extract has the effects of activating blood and removing blood stasis, anti-inflammatory, antioxidant and the like. It can improve skin blood circulation, promote skin metabolism, remove free radicals, inhibit melanin deposition, accelerate depigmentation and ultraviolet absorption, and has the effects of whitening, sunscreen and anti-aging

[0028] Dendrobium extract has the effects of antibacterial, anti-inflammatory and enhancing immunity, and can repair skin follicle damage and hair growth. It has the effects of regulating the balance of yin and yang in the body and restoring sub-health.

[0029] The beneficial effects achieved by the present application are:

[0030] The hair growth agent of the present application contains stem cell growth factors, NMN metabolic energy substances, and is supplemented with coenzyme Q10, melatonin, ginsenoside A2, chuanxiong extract, safflower extract, dendrobium extract, radix falcaliae polygoni multiflori extract and ligustrum lucidum extract. It can promote hair follicle cell growth and hair growth, improve cell metabolism, increase blood supply to hair follicles, and significantly increase hair growth in one to three months, thereby relieving the pain of patients with hair loss.

[0031] The various growth factors, gene regulatory factors and the like in the stem cell extract can promote tissue cell reprogramming and reverse differentiation and stem cell proliferation, promote epidermal cell growth, hair follicle cell hair growth, dermal deep collagen expression, promote capillary proliferation, tissue organ cell and immune cell proliferation, whole body cell metabolic activity enhancement, and anti-aging and anti-apoptosis effects. Nicotinamide mononucleotide has the effects of promoting metabolism and energy substance (ATP) generation, and has the effect of promoting tissue vitality. At the same time, it is supplemented with coenzyme Q10, melatonin, ginsenoside A2, chuanxiong extract, safflower extract, dendrobium extract, radix falcaliae polygoni multiflori extract and ligustrum lucidum extract, which can prevent hair loss and promote hair follicle cell growth and hair growth. DETAILED DESCRIPTION

[0032] The present application will be further described below. The following examples are only used to more clearly illustrate the technical solutions of the present application, and cannot be used to limit the protection scope of the present application.

[0033] Example 1

[0034] A hair tonic containing stem cell extract, 100 mL of hair tonic includes the following raw materials: stem cell extract freeze-dried powder 50 mg, beta nicotinamide mononucleotide 100 mg, coenzyme Q10 10 mg, melatonin 10 mg, ginsenoside A2 10 mg, chuanxiong extract 0.2 g, safflower extract 0.2 g, dendrobium extract 0.3 g, radix falcaliae extract 1 g, ligustrum lucidum extract 1 g, and the rest is deionized water.

[0035] The stem cell extract refers to the extract of Turritopsis nutricula stem cells.

[0036] Stem cells are cells that have the ability to regenerate and self-renew, that is, cells that have the ability to proliferate, self-repair, mass produce, and differentiate into offspring. They are of great significance to the repair of necrotic or diseased tissues in the human body and the replacement of aging apoptotic cells. However, human stem cell culture technology is complex and expensive, with a cost of tens of millions of yuan for a treatment. However, the stem cells of animals and plants, especially Turritopsis nutricula stem cells, can be cultured in seawater, which is low-cost and can be mass-produced, widely used, and truly make the benefits of stem cells available to ordinary people.

[0037] The Turritopsis nutricula stem cells used in this embodiment are extracted from the juvenile stage of the polyp (Hydra) (see the applicant's patent: CN105920044B, a polyp stem cell preparation and its preparation method and application).

[0038] Turritopsis nutricula is a coelenterate, and its body is mainly composed of stem cells. Current research has found that Turritopsis nutricula is naturally capable of "rejuvenation" and is the only immortal organism in the world today. Turritopsis nutricula "rejuvenates" through a process of transdifferentiation, i.e., from an old age to a young type. Therefore, Turritopsis nutricula's life is endless. Turritopsis nutricula returns to the beginning of life before death and reinterprets its growth and development.

[0039] Turritopsis nutricula is immortal because it has a regeneration gene. If this gene, which can cause aging cells to reverse differentiation into stem cells, and its differentiation-regulating cytokines are absorbed from skin hair follicles into subcutaneous tissue cells, human cell genes can be regulated to reverse differentiation, making human cells younger and stem cells, and making the skin rejuvenate.

[0040] Lighthouse jellyfish are extremely rich in collagen. The value of the collagen of the Lighthouse jellyfish is incomparable. Let's listen to the conclusions of scientists. Professor Piraino, a biologist at the University of Salento in Lecce, Italy, said that it has been proven that jellies are useful to us (from medicines to nutritional products and even healthy and delicious foods). Take collagen as an example. It is the main structural protein of human connective tissue and can also be used as a scaffold for bone transplantation and plastic surgery (medical aesthetics) to be implanted into the human body. But not all types of animal collagen are compatible with all types of human cells. This is because the collagen of jellyfish is primitive and compatible with a wide variety of human cell types.

[0041] He said that if you break collagen into small pieces, it will act as an antioxidant, a molecule that is deemed healthy because it helps soak up chemicals called radicals that can damage cells.

[0042] He also isolated a compound from jellyfish that appears to have anticancer activity. He is now working to find out whether the compound is effective against a range of different cancers.

[0043] (Reference: by Caled Davies, From Horizon Magazine, Horizon: The EU Research & Innovation Magazine).

[0044] The Chuanxiong extract is obtained by the following steps: Chuanxiong is placed in water under the condition of reflux extraction at a temperature of 95℃ to obtain water extract, and the extraction is performed twice, and the water extract obtained in the two times is combined, concentrated and dried to obtain the Chuanxiong extract in the form of paste.

[0045] The safflower extract is obtained by the following steps: safflower is crushed and extracted with 75v / v% ethanol aqueous solution under the condition of a temperature of 35℃ to obtain ethanol extract, the ethanol extract is concentrated, passed through a macroporous resin, eluted with 85% ethanol aqueous solution, concentrated and freeze-dried to obtain the safflower extract.

[0046] The Dendrobium extract is obtained by the following steps: Dendrobium is crushed and placed in water under the condition of extraction at a temperature of 95℃ to obtain water extract, the water extract is concentrated, dissolved in 95v / v% ethanol aqueous solution, passed through an HLB column, eluted with ethanol, concentrated and freeze-dried to obtain the Dendrobium extract.

[0047] The Radix Polygoni Multiflori extract is obtained by the following steps: Radix Polygoni Multiflori is placed in water under the condition of extraction at a temperature of 95℃ to obtain extract, the extract is concentrated, ethanol is added, and the alcohol precipitate is collected after standing for 24-48h, and the alcohol precipitate is freeze-dried to obtain the Radix Polygoni Multiflori extract.

[0048] The Ligustri Lucidi Fructus extract is obtained by the following steps: Ligustri Lucidi Fructus is crushed and extracted with 75v / v% ethanol under the condition of reflux extraction at a temperature of 65℃ to obtain ethanol extract, the ethanol extract is concentrated, passed through a macroporous resin, eluted with 85% v / v ethanol aqueous solution, concentrated and dried to obtain the Ligustri Lucidi Fructus extract.

[0049] A method for using a hair restorer containing stem cell extract, the hair restorer is sterilized with a 0.22μm sterile filter, and the color is removed by ultrafiltration, and then the hair restorer is applied to the scalp or used for washing hair.

[0050] Example 2

[0051] A hair restorer containing stem cell extract, 100mL of the hair restorer includes the following raw materials: stem cell extract freeze-dried powder 150mg, β nicotinamide mononucleotide (NMN) 325mg, coenzyme Q1060mg, melatonin 110mg, ginsenoside A2120mg, Chuanxiong extract 1g, safflower extract 1g, Dendrobium extract 1.5g, Radix Polygoni Multiflori extract 4g, Ligustri Lucidi Fructus extract 4g, and the rest is deionized water.

[0052] The stem cell extract refers to the extract of Aequorea victoria stem cells.

[0053] The extract of Aequorea victoria stem cells is obtained by the following steps:

[0054] (1) Cleanly feed the living hydra for 24-48 hours, rinse repeatedly with sterile normal saline, disinfect with 70% alcohol, remove the skin and tentacles under sterile conditions at 4°C, crush and filter, add cell separation digestion solution and buffer culture solution to prepare a single cell suspension, wash with normal saline by centrifugation three times, add an appropriate amount of culture medium, and incubate in a 37°C carbon dioxide incubator for 45 minutes, and then in an ice bath at 4°C overnight;

[0055] (2) Use a flow cytometer to separate stem cells from the cell suspension with the stem cell marker, collect the stem cells, centrifuge and wash three times under sterile conditions at 4°C, remove the marker, and add buffer to prepare a cell suspension;

[0056] (3) Add the cell suspension to pre-cooled buffer under sterile conditions and ultrasonically break the cells, centrifuge at 4°C, collect the supernatant, measure the protein concentration, and prepare a stem cell extract containing 1-2×108 cells per ml;

[0057] (4) Mix the obtained stem cell extract with albumin, normal saline mixture, and excipients to obtain the product.

[0058] The extract of Chuanxiong is obtained by the following steps: Chuanxiong is placed in water and extracted by reflux at a temperature of 95°C to obtain a water extract, and the extraction is performed twice. The water extracts obtained in the two extractions are combined, concentrated, and dried to obtain a paste-like Chuanxiong extract.

[0059] The extract of Honghua is obtained by the following steps: Honghua is crushed and extracted with 75v / v% ethanol aqueous solution at a temperature of 35°C to obtain an ethanol extract. The ethanol extract is concentrated, passed through a macroporous resin, eluted with 85% ethanol aqueous solution, concentrated, and freeze-dried to obtain the extract of Honghua.

[0060] The extract of Shihud is obtained by the following steps: Shihud is crushed and extracted with water at a temperature of 95°C to obtain a water extract. The water extract is concentrated, dissolved in 95v / v% ethanol aqueous solution, passed through an HLB column, eluted with ethanol, and freeze-dried to obtain the extract of Shihud.

[0061] The extract of HeShouWu is obtained by the following steps: HeShouWu is placed in water and extracted at a temperature of 95°C to obtain an extract. The extract is concentrated, added with ethanol, and left to stand for 24-48 hours. The alcohol precipitate is collected and freeze-dried to obtain the extract of HeShouWu.

[0062] The extract of Ligustrum lucidum is obtained by the following steps: crushing Ligustrum lucidum, then extracting with 75% v / v ethanol at a temperature of 65°C to obtain an ethanol extract, concentrating the ethanol extract, then passing through a macroporous resin, eluting with 85% v / v ethanol aqueous solution, and drying after concentration to obtain the extract of Ligustrum lucidum.

[0063] A method for using a hair restorer containing stem cell extract, the hair restorer is sterilized by 0.22 μm sterile filter membrane, and the color is removed by ultrafiltration, and then the hair restorer is applied to the scalp of the alopecia area or used for washing hair.

[0064] Example 3

[0065] A hair restorer containing stem cell extract, 100 mL of the hair restorer comprises the following raw materials: 250 mg of stem cell extract freeze-dried powder, 650 mg of beta nicotinamide mononucleotide, 120 mg of coenzyme Q10, 220 mg of melatonin, 240 mg of ginsenoside A2, 2 g of Chuanxiong extract, 2 g of safflower extract, 3 g of dendrobium extract, 8 g of radix falcaliae extract, 8 g of Ligustrum lucidum extract, and the rest is deionized water.

[0066] The stem cell extract refers to an extract of jellyfish stem cell.

[0067] The extract of jellyfish stem cell is obtained by the following steps:

[0068] (1) Cleanly feeding the living hydra for 24-48 hours, repeatedly washing with sterile normal saline, disinfecting with 70% alcohol, removing the skin and tentacles under sterile conditions at 4°C, crushing and filtering, adding cell separation and digestion solution and buffer culture solution to prepare a single cell suspension, washing with normal saline for 3 times by centrifugation, adding an appropriate amount of culture medium, and incubating in a 37°C carbon dioxide incubator for 45 minutes with a stem cell marker antibody with a selection marker, and then incubating overnight in an ice bath at 4°C;

[0069] (2) Separating the stem cells from the cell suspension with the stem cell marker by using a flow cytometer, centrifuging and washing the stem cells for 3 times under sterile conditions at 4°C, removing the marker, and adding a buffer to prepare a cell suspension;

[0070] (3) Adding the cell suspension to pre-cooled buffer under sterile conditions to break the cells by ultrasonic, centrifuging at 4°C, collecting the supernatant, measuring the protein concentration, and preparing a stem cell extract containing 1-2×108 cells per ml;

[0071] (4) Mixing the obtained stem cell extract with albumin, normal saline mixture, and excipients uniformly to obtain the stem cell extract.

[0072] The extract of Chuanxiong is obtained by the following steps: Chuanxiong is put into water to extract under the condition of 95℃, and the water extract is obtained, the extraction is carried out twice, and the water extract is combined and concentrated, and then dried to obtain the extract of Chuanxiong in paste form.

[0073] The extract of Honghua is obtained by the following steps: Honghua is crushed and extracted with 75v / v% ethanol water solution under the condition of 35℃, and the ethanol extract is obtained, the ethanol extract is concentrated, and then passed through a macroporous resin, eluted with 85% ethanol water solution, concentrated, and then freeze-dried to obtain the extract of Honghua.

[0074] The extract of Shihud is obtained by the following steps: Shihud is crushed and put into water to extract under the condition of 95℃, and the water extract is obtained, the water extract is concentrated, and then dissolved in 95v / v% ethanol water solution, passed through an HLB column, eluted with ethanol, concentrated, and then freeze-dried to obtain the extract of Shihud.

[0075] The extract of Heshouwu is obtained by the following steps: Heshouwu is put into water to extract under the condition of 95℃, and the extract is obtained, the extract is concentrated, and then ethanol is added, and the alcohol precipitate is collected after standing for 24-48h, and the alcohol precipitate is freeze-dried to obtain the extract of Heshouwu.

[0076] The extract of Nuzhizi is obtained by the following steps: Nuzhizi is crushed and extracted with 75v / v% ethanol under the condition of 65℃, and the ethanol extract is obtained, the ethanol extract is concentrated, and then passed through a macroporous resin, eluted with 85%v / v ethanol water solution, concentrated, and then dried to obtain the extract of Nuzhizi.

[0077] A method for using a hair restorer containing a stem cell extract, the hair restorer is sterilized with a 0.22μm sterile filter, and the color is removed by ultrafiltration, and then applied or washed on the scalp of the hair loss site.

[0078] Clinical trial scheme and results:

[0079] 80 patients with hair loss (diagnosed as seborrheic alopecia, alopecia, endocrine alopecia, and nutritional alopecia) are randomly selected, the age of the volunteers is controlled to be 35-55 years old, and the 80 volunteers are randomly divided into 4 groups, 20 people in each group, among which three groups of volunteers correspondingly use the hair restorer of the above embodiments 1-3 to apply on the hair loss site, and the fourth group of volunteers applies normal saline, and the four groups of volunteers each apply once in the morning and evening, and insist on using for 28 days.

[0080] The examples and comparative examples were evaluated, and the grades were divided into 1-5. The hair loss prevention rate 5 indicates that the hair stops falling out, 4 indicates that the hair loss is reduced by >70%, 3 indicates that the hair loss is reduced by >50%, 2 indicates that the hair loss is reduced by >30%, and 1 indicates that there is basically no effect. The growth promotion rate 5 indicates that the new hair grows well, 4 indicates that the new hair regrowth is >70%, 3 indicates that the new hair regrowth is >50%, 2 indicates that the new hair regrowth is >30%, and 1 indicates that the new hair growth is <30%. The average score was calculated, and the results are shown in Table 1.

[0081] Table 1: Score of each group

[0082] Example 1 Example 2 Example 3 Control group Hair growth promotion rate 2.8±0.2* 3.7±0.1** 4.8±0.2*** 1.1±0.1 Hair loss prevention rate 2.6±0.1* 3.5±0.2** 4.7±0.1*** 1.2±0.1

[0083] Note: *P<0.05; **P<0.01; ***P<0.001

[0084] From the results in Table 1, it can be seen that the hair growth agent of the present application has a significant effect on hair loss prevention and hair growth promotion compared with the control group. After using the hair growth agents of examples 1-3 of the present application, the hair follicle cell growth and hair growth can be promoted, the cell metabolism can be improved, the blood supply of the hair follicle can be increased, and the hair growth agent can be used for a long time.

[0085] The above only describes the preferred embodiments of the present application, and it should be noted that for ordinary skilled persons in the art, several improvements and modifications can be made without departing from the technical principles of the present application, and these improvements and modifications should also be considered as the protection scope of the present application.

Claims

1. A hair restorer containing a stem cell extract, characterized in that, 100 mL of the hair growth agent comprises the following raw materials: 50-250 mg of dry cell extract powder, 100-650 mg of beta nicotinamide mononucleotide, 10-120 mg of coenzyme Q10, 10-220 mg of melatonin, 10-240 mg of ginsenoside A2, 0.2-2 g of chuanqiong extract, 0.2-2 g of safflower extract, 0.3-3 g of dendrobium extract, 1-8 g of he shou wu extract, 1-8 g of ligustrum extract, and the rest is deionized water. The dry cell extract is a jellyfish dry cell extract obtained by the following steps: (1) Clean and feed the living medusa for 24-48 hours, rinse repeatedly with sterile normal saline, disinfect with 70% alcohol, remove the skin and tentacles under sterile conditions at 4°C, crush and filter, add cell separation and digestion solution and buffer culture solution to prepare a single cell suspension, wash with normal saline for 3 times, add an appropriate amount of culture medium, incubate with a dry cell marker antibody with a selection marker in a 37°C carbon dioxide incubator for 45 minutes, and incubate overnight in an ice bath at 4°C. (2) Use a flow cytometer to separate the dry cells from the cell suspension with the dry cell marker, collect the dry cells, centrifuge and wash for 3 times under sterile conditions at 4°C, remove the marker, and add buffer to prepare a cell suspension. (3) The cell suspension is added to a pre-cooled buffer under sterile conditions and the cells are broken up by ultrasonic waves, centrifuged at 4°C, and the supernatant is collected, the protein concentration is measured, and a stem cell extract containing 1-2 x 10 8 cells per ml is prepared; (4) Mix the obtained dry cell extract with albumin, normal saline mixture, and excipients uniformly to obtain the hair growth agent.

2. The hair restorer containing stem cell extract according to claim 1, characterized in that, 100 mL of the hair growth agent comprises the following raw materials:

3. The hair restorer containing stem cell extract according to claim 1 or 2, characterized in that, The chuanqiong extract is obtained by the following steps: placing chuanqiong in water and performing reflux extraction under the condition of a temperature of 90-100°C to obtain a water extract, extracting twice, combining and concentrating the water extracts, and drying to obtain a paste-like chuanqiong extract.

4. The hair restorer containing stem cell extract according to claim 1 or 2, characterized in that, The safflower extract is obtained by the following steps: crushing safflower and performing extraction with 70-80 v / v% ethanol water solution under the condition of a temperature of 30-40°C to obtain an ethanol extract, concentrating the ethanol extract, passing through a macroporous resin, eluting with 80-90% ethanol water solution, and freeze-drying after concentration to obtain the safflower extract.

5. The hair restorer containing stem cell extract according to claim 1 or 2, characterized in that, The dendrobium extract is obtained by the following steps: crushing dendrobium and placing it in water and performing extraction under the condition of a temperature of 90-100°C to obtain a water extract, dissolving the water extract with 95 v / v% ethanol water solution after concentration, passing through an HLB column, eluting with ethanol, and freeze-drying after concentration to obtain the dendrobium extract.

6. The hair restorer containing stem cell extract according to claim 1 or 2, wherein the stem cell extract is obtained from the umbilical cord blood. The extract of Radix Polygoni Multiflori is obtained by the following steps: placing Radix Polygoni Multiflori in water to extract at a temperature of 90-100 DEG C to obtain an extract, adding ethanol to the extract after concentration, collecting the alcohol precipitate after standing for 24-48 hours, and freeze-drying the alcohol precipitate to obtain the extract of Radix Polygoni Multiflori.

7. The hair restorer containing stem cell extract according to claim 1 or 2, wherein the stem cell extract is obtained from the umbilical cord blood. The extract of Fructus Ligustri Lucidi is obtained by the following steps: crushing Fructus Ligustri Lucidi, reflux extracting the crushed Fructus Ligustri Lucidi with 70-80 v / v% ethanol at a temperature of 60-70 DEG C to obtain an ethanol extract, passing the ethanol extract through a macroporous resin after concentration, eluting the macroporous resin with 80-90 v / v% ethanol, and drying the eluate after concentration to obtain the extract of Fructus Ligustri Lucidi.