A dry blood analysis device for sample interpretation based on area array CMOS

Abstract

The present invention discloses a dry blood analysis device for sample interpretation based on area array CMOS, which belongs to the technical field of medical devices, including a housing, an upper cover hinged on the housing, a touch display screen arranged inside the upper cover, a detection chamber arranged inside the housing, a detection chamber cover hinged on the detection chamber, a movement installed inside the detection chamber, the movement including a movement support cover plate, a detection test tube access port arranged on the movement support cover plate, a test tube loading mobile platform arranged below the detection test tube access port, an image acquisition device arranged on one side of the test tube loading mobile platform, a blue light source arranged on both sides of the image acquisition device, a test tube rack and a test tube steering device arranged on the test tube loading mobile platform, the test tube steering device arranged at the end position of the test tube rack, and the test tube steering device and the test tube rack are arranged coaxially. The present invention has the advantages of simple and compact structure, small size, simple operation, small blood collection amount, extremely small use of consumables, fast detection speed and good detection effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices, and in particular relates to a dry blood analysis device for sample interpretation based on area array CMOS. Background Art

[0002] The number of blood cells is of great significance in the diagnosis of clinical diseases. Abnormal production and destruction of blood cells due to various reasons will cause changes in the quantity or quality of blood cells, thus leading to the occurrence of diseases.

[0003] The existing blood cell counting methods are mainly blood cell counting method and flow cytometer counting method. The blood cell counting method requires human eye counting, which is not only cumbersome and time-consuming, but may also cause reading errors due to uneven distribution of blood cells; while the flow cytometer counting method is fast and accurate, but the instrument is expensive. Therefore, there is an urgent need to develop a convenient and highly accurate blood cell counting device. Summary of the invention

[0004] The problem to be solved by the present invention is to provide a dry blood analysis device for sample interpretation based on area array CMOS, which has a simple and compact structure, small size, easy operation, small blood collection volume, minimal consumables, fast detection speed and good detection effect.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is: a dry blood analysis device for sample interpretation based on area array CMOS, comprising a shell, an upper cover is hinged on the shell, a touch display screen is arranged on the inner side of the upper cover, a detection chamber is arranged inside the shell, a detection chamber cover is hinged on the detection chamber, a movement is installed inside the detection chamber, the movement comprises a movement support cover plate, a detection test tube access port is arranged on the movement support cover plate, a test tube loading mobile platform is arranged below the detection test tube access port, an image acquisition device is arranged on one side of the test tube loading mobile platform, blue light sources are arranged on both sides of the image acquisition device, a test tube rack and a test tube steering device are arranged on the test tube loading mobile platform, the test tube steering device is arranged at the end position of the test tube rack, and the test tube steering device is arranged coaxially with the test tube rack.

[0006] A main control board is also arranged in the upper cover, and the main control board is located behind the touch display screen. A driving board and a battery are also arranged in the shell, and the driving board and the battery are located behind the movement.

[0007] A tray is arranged at the bottom of the shell.

[0008] The test tube loading mobile platform is connected to the guide rail via a slider, and the test tube loading mobile platform is driven by a mobile motor to move along the guide rail.

[0009] The test tube steering device is provided with a test tube positioning cavity at one end facing the test tube rack, and a plurality of interconnected guide slide grooves are evenly arranged on the outer wall of the test tube steering device. A spring sheet is provided on one side of the test tube steering device, and a slide groove ejector pin is installed on the spring sheet. The slide groove ejector pin is arranged in cooperation with the guide slide groove to drive the test tube steering device to rotate.

[0010] The test tube steering device is rotatably connected with a steering device bracket on the test tube loading mobile platform through a bearing.

[0011] The support end of the test tube rack is U-shaped.

[0012] The guide slide groove is in a transverse Y-shape.

[0013] The test tube adopts a transparent capillary tube, and a color developer layer is attached to the inner wall of the test tube.

[0014] The image acquisition device includes a CMOS image sensor and a lens module.

[0015] A focusing lens and a filter are arranged on the outside of the image acquisition device.

[0016] A focusing lens and a filter are arranged outside the blue light source.

[0017] Due to the adoption of the above technical scheme, the present invention has a simple and compact structure. When in use, a transparent capillary test tube containing blood is centrifuged and then placed on the test tube rack of the test tube loading mobile platform, one end of the test tube is inserted into the test tube positioning cavity of the test tube steering device and clamped, and the rotational motion of the mobile motor is converted into linear motion by a lead screw, thereby driving the test tube loading mobile platform to move left and right. At the same time, the guide slide groove moves along the slide groove ejector pin to realize the rotation of the test tube steering device, and the horizontal movement and rotation of the test tube are finally realized by starting and stopping a motor, and the images of different positions of the test tube are collected at the detection position by the image acquisition device, and the blood item data are obtained after processing.

[0018] During the detection process, blue light is used for irradiation. During the irradiation, the test tube rotates a certain angle along its axis, and then the image is collected. Multi-angle sampling can avoid errors caused by factors such as uneven blood layers on the sampling surface. It is convenient to use and improves the detection speed and detection effect.

[0019] The invention has the advantages of simple and compact structure, small volume, easy operation, small amount of blood drawn, minimal use of consumables, fast detection speed and good detection effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention will be described in detail below with reference to the accompanying drawings and in combination with examples, and the advantages and implementation methods of the present invention will become more obvious. The contents shown in the accompanying drawings are only used to explain the present invention and do not constitute any limitation to the present invention in any sense. In the accompanying drawings:

[0021] Figure 1 It is a structural schematic diagram of the present invention

[0022] Figure 2 Schematic diagram of the internal structure of the housing of the present invention

[0023] Figure 3 It is a schematic diagram of the structure of the movement of the present invention.

[0024] Figure 4 This is a schematic diagram of the internal structure of the movement of the present invention.

[0025] Figure 5 This is the test tube detection principle diagram of the present invention

[0026] In the figure:

[0027] 1. Top cover 2. Touch screen 3. Shell

[0028] 4. Equipment switch 5. Inspection compartment cover 6. Inspection test tube access port

[0029] 7. Movement 8. Tray 9. Main control board

[0030] 10. Driving board 11. Battery 12. Test tube

[0031] 701, movement support cover 702, image acquisition device 703, blue light source

[0032] 704, test tube loading mobile platform 705, test tube steering device 706, mobile motor

[0033] 707, test tube rack 708, spring sheet 709, slide thimble

[0034] 710, guide chute 711, steering device bracket DETAILED DESCRIPTION

[0035] like Figures 1 to 5As shown, the present invention is a dry blood analysis device for sample interpretation based on area array CMOS, comprising a housing 3, an upper cover 1 is hingedly connected to the housing 3, a touch display screen 2 is arranged on the inner side 1 of the upper cover, a detection chamber is arranged inside the housing 3, a detection chamber cover 5 is hingedly connected to the detection chamber, a movement 7 is installed inside the detection chamber, the movement 7 comprises a movement support cover plate 701, a detection test tube access port 6 is arranged on the movement support cover plate 701, a test tube loading mobile platform 704 is arranged below the test tube access port 6, and an image acquisition Device 702, blue light sources 703 are arranged on both sides of the image acquisition device 702, a test tube rack 707 and a test tube steering device 705 are arranged on the test tube loading mobile platform 704, the test tube steering device 705 is arranged at the end position of the test tube rack 707, and the test tube steering device 705 is coaxially arranged with the test tube rack 707, a main control board 9 is also arranged in the upper cover 1, and the main control board 9 is located behind the touch display screen 2, a driving board 10 and a battery 11 are also arranged in the shell 3, and the driving board 10 and the battery 11 are located behind the movement 7, and a tray 8 is arranged at the bottom of the shell 3.

[0036] The test tube loading mobile platform 704 is connected to the guide rail through a slider, and the test tube loading mobile platform 704 is driven by a mobile motor 706 to move along the guide rail. The test tube steering device 705 is provided with a test tube positioning cavity at one end facing the test tube rack 707. The outer wall of the test tube steering device 707 is evenly provided with a plurality of interconnected guide slots 710. A spring sheet 708 is provided on one side of the test tube steering device 705, and a slot ejector pin 709 is installed on the spring sheet 708. The slot ejector pin 709 is connected to the guide slot 710. The test tube steering device 705 is driven to rotate by the coordinated setting. The test tube steering device 705 is rotatably connected with the steering device bracket 711 on the test tube loading mobile platform 704 through the bearing. The support end of the test tube rack 707 is U-shaped, and the guide slide groove 710 is horizontally Y-shaped. The test tube 12 adopts a transparent capillary tube, and a color developer layer is attached to the inner wall of the test tube 12. The image acquisition device 702 includes a CMOS image sensor and a lens module. A focusing lens and a filter are arranged on the outside of the image acquisition device 702. A focusing lens and a filter are arranged on the outside of the blue light source 703.

[0037] The detection principle of the present invention is to perform electronic optical linear measurement on the discrete stratification of the packed blood cells in the capillary. The blood forms a density gradient during high-speed centrifugation, which leads to cell stratification. Based on this, nine main hematological parameters such as platelet count are analyzed (for the detection of hematocrit HCT (%), hemoglobin HGB (g / L), total white blood cell count WBC (109 / L), granulocyte value #GRAN (109 / L), lymphomonocyte value #LY / MO (109 / L), granulocyte percentage %GRAN, lymphomonocyte percentage %LY / MO, platelet PLT (109 / L), and mean red blood cell hemoglobin concentration MCHC (g / L) in in vitro blood routine detection). A diagnostic report is obtained, and the detection value is compared with the hematological data in the device. A preliminary diagnostic analysis will be performed for abnormal parameters. The detection process is fully automated, and the operator only needs to prepare the sample test tube and insert the test tube into the device. The result can be obtained after about 1 minute, which greatly saves the detection time.

[0038] The workflow of the present invention is:

[0039] First, the blood sample is pre-treated by centrifugation. A conventional centrifugal device can be used. According to the differences in the density of various blood cells, a certain amount of blood to be tested is sucked into a capillary tube with a color developer layer. The blood cells are separated in different areas of the capillary tube at a relative centrifugal speed of 12000r / min for 5 minutes to form a narrow equilibrium zone and maintain relative stability. After the sampling capillary tube is centrifuged, the blood cells can be stratified from top to bottom into plasma, platelets, lymphocytes and monocytes, granulocytes, and red blood cells. The cell staining technology-acridine orange fluorescence staining is applied, because acridine orange can dye platelets light yellow, lymphocytes and monocytes bright green, and granulocytes orange. In addition to the EDTA-K2 anticoagulant in the capillary tube, there is also a certain amount of acridine orange on its inner wall, so the color of each cell layer is different after centrifugation. Under the action of the dye, granulocytes are orange, lymphocytes and monocytes are green, platelets are light yellow, and red blood cells are not colored.

[0040] Then, the upper cover 1 of the present invention is opened, and the device switch 4 is pressed to start the device for self-test. After the self-test is completed, the detection chamber cover 5 is opened to put the centrifuged capillary into the detection test tube access port 6, and the detection chamber cover 5 is closed to start the test.

[0041] At the beginning of the test, one end of the capillary on the test tube loading mobile platform 704 is inserted into the test tube positioning cavity of the test tube steering device 705 and clamped, and then moved to the test position to display different colors through blue light excitation. The image is collected by the camera, and the CMOS image sensor performs image processing to find the layer boundary, and the actual size corresponding to each layer can be obtained, thereby obtaining PLT, HCT, HGB, and WBC data.

[0042] The blue light source is used for fluorescence scanning of the yellowish layer. The filter (interference filter) is used to shield all light with a wavelength of 490 nanometers or longer. The focusing lens (aspherical condenser lens) is used to focus the light energy on the capillary. In order to maintain a stable relative position relative to the image acquisition device 702, the blue light source is mechanically mounted so that the capillary moving along its longitudinal axis remains perpendicular to the light source during the entire scanning process.

[0043] After the test is completed, the capillary tube is pushed out, and the test tube loading moving platform 704 moves to the test tube taking and placing port 6 to take out the capillary test tube.

[0044] The embodiments of the present invention are described in detail above, but the contents described are only preferred embodiments of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of this patent.

Claims

1. A dry blood analysis device for sample interpretation based on area array CMOS, characterized in that: It comprises a shell, an upper cover is hingedly connected to the shell, a touch display screen is arranged inside the upper cover, a detection chamber is arranged inside the shell, a detection chamber cover is hingedly connected to the detection chamber, a movement is installed inside the detection chamber, the movement comprises a movement support cover plate, a detection test tube access port is arranged on the movement support cover plate, a test tube loading mobile platform is arranged below the detection test tube access port, an image acquisition device is arranged on one side of the test tube loading mobile platform, blue light sources are arranged on both sides of the image acquisition device, a test tube rack and a test tube steering device are arranged on the test tube loading mobile platform, the test tube steering device is arranged at the end position of the test tube rack, and the test tube steering device is arranged coaxially with the test tube rack; The test tube loading mobile platform is connected to the guide rail via a slider, and the test tube loading mobile platform is driven by a mobile motor to move along the guide rail; The test tube steering device is provided with a test tube positioning cavity at one end facing the test tube rack, and a plurality of interconnected guide slide grooves are evenly provided on the outer wall of the test tube steering device, and the guide slide grooves are in a transverse Y-shape. A spring sheet is provided on one side of the test tube steering device, and a slide groove ejector pin is installed on the spring sheet, and the slide groove ejector pin is arranged in cooperation with the guide slide groove to drive the test tube steering device to rotate; The image acquisition device includes a CMOS image sensor and a lens module; A focusing lens and a filter are arranged on the outside of the image acquisition device; A focusing lens and a filter are arranged outside the blue light source.

2. The dry blood analysis device for sample interpretation based on area array CMOS according to claim 1, characterized in that: A main control board is also arranged in the upper cover, and the main control board is located behind the touch display screen. A driving board and a battery are also arranged in the shell, and the driving board and the battery are located behind the movement. A tray is arranged at the bottom of the shell.

3. The dry blood analysis device for sample interpretation based on area array CMOS according to claim 1, characterized in that: The test tube steering device is rotatably connected with a steering device bracket on the test tube loading mobile platform through a bearing.

4. The dry blood analysis device for sample interpretation based on area array CMOS according to claim 1, characterized in that: The support end of the test tube rack is U-shaped.

5. The dry blood analysis device for sample interpretation based on area array CMOS according to claim 1, characterized in that: The test tube adopts a transparent capillary tube, and a color developer layer is attached to the inner wall of the test tube.

Images