Methods for detecting TCO2 and TCa in blood, fluidization system and electrolyte analyzer

Abstract

This invention discloses a method, liquid circuit system, and electrolyte analyzer for detecting TCO2 and TCa in blood. The method for detecting TCO2 and TCa in blood includes the following steps: providing a sample and a diluent; mixing the sample and the diluent to react and generate a mixture containing carbon dioxide gas and calcium ion complexes; detecting the carbon dioxide gas content; and detecting the calcium ion concentration in the mixture to obtain the total calcium concentration. In this invention, by mixing the diluent with the sample to generate carbon dioxide gas and calcium ion complexes, TCa can be directly tested after TCO2 detection. The mixing and reaction of the diluent with the sample is equivalent to diluting the sample once, allowing for testing with a small sample size, reducing the sample volume required for detection, and eliminating the need for additional reaction processes between the two tests, thus significantly shortening the detection time.

Description

Technical Field

[0001] This invention relates to the field of blood testing and analysis technology, and in particular to a method, liquid circuit system and electrolyte analyzer for detecting TCO2 and TCa in blood. Background Technology

[0002] Analyzing total carbon dioxide (TCO2) in blood is an important means of understanding alveolar ventilation and gas exchange function, and adding TCO2 detection to electrolyte analyzers has high clinical significance. Current TCO2 detection methods all use pressure methods and directly test blood samples, requiring large sample volumes. Summary of the Invention

[0003] The main objective of this invention is to propose a method, liquid circuit system, and electrolyte analyzer for the detection of TCO2 and TCa in blood, aiming to solve the problem that existing TCO2 detection methods require a large sample volume.

[0004] To achieve the above objectives, this invention proposes a method for detecting TCO2 and TCa in blood, comprising the following steps:

[0005] Provide samples and diluents;

[0006] The sample and the diluent are mixed to cause the sample and the diluent to react and generate carbon dioxide gas and a mixture containing calcium ion complexes;

[0007] The carbon dioxide gas content was detected;

[0008] The concentration of calcium ions in the mixture is measured to obtain the total calcium concentration.

[0009] Optionally, the diluent includes citric acid and Tris.

[0010] The present invention also provides a liquid circuit system for an electrolyte analyzer, comprising:

[0011] Sample supply pipeline;

[0012] A diluent supply pipe is provided for supplying citric acid diluent;

[0013] The reaction vessel has an inlet pipe, a gas phase outlet, and a liquid phase outlet, wherein the inlet pipe is connected to the sample supply pipe and the diluent supply pipe;

[0014] A first detector, connected to the gas phase outlet, is used to detect the carbon dioxide content in the gas phase; and,

[0015] The second detector is connected to the liquid phase outlet and is used to detect the concentration of calcium ions in the liquid phase;

[0016] Both the sample inlet pipe and the liquid phase outlet can be shut off.

[0017] Optionally, the liquid phase outlet and the second detector are connected via a liquid phase discharge pipe;

[0018] The liquid phase discharge pipe is equipped with a rotary valve, and the sample inlet pipe is equipped with a first pinch valve.

[0019] Optionally, the first detector includes a pressure sensor.

[0020] Optionally, the reaction vessel is further provided with a pressure regulating device to regulate the gas pressure in the reaction vessel.

[0021] Optionally, the second detector includes an electrode box.

[0022] Optionally, it also includes a mixing tank, which is connected to the sample inlet pipe, and the mixing tank is provided with the sample supply pipe and the diluent supply pipe.

[0023] Optionally, it also includes:

[0024] A cleaning fluid supply device for supplying cleaning fluid, the cleaning fluid supply device being connected to the mixing tank via an oil pump; and...

[0025] A peristaltic pump is installed in the sample inlet pipe and located between the mixing tank and the reaction vessel.

[0026] The present invention also provides an electrolyte analyzer, including the liquid circuit system of the electrolyte analyzer described above, wherein the liquid circuit system of the electrolyte analyzer includes:

[0027] Sample supply pipeline;

[0028] A diluent supply pipe is provided for supplying citric acid diluent;

[0029] The reaction vessel has an inlet pipe, a gas phase outlet, and a liquid phase outlet, wherein the inlet pipe is connected to the sample supply pipe and the diluent supply pipe;

[0030] A first detector, connected to the gas phase outlet, is used to detect the carbon dioxide content in the gas phase; and,

[0031] The second detector is connected to the liquid phase outlet and is used to detect the concentration of calcium ions in the liquid phase;

[0032] Both the sample inlet pipe and the liquid phase outlet can be shut off.

[0033] In the technical solution of the present invention, by mixing the diluent with the sample to generate carbon dioxide gas and calcium ion complex, TCa can be directly tested after TCO2 is detected. Mixing the diluent with the sample is equivalent to diluting the sample once, and a small amount of sample can be used to complete the test, which helps to reduce the amount of sample required for detection. Moreover, no additional reaction process is required between the two tests, which greatly shortens the detection time. Attached Figure Description

[0034] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0035] Figure 1 A schematic flowchart of an embodiment of the method for detecting TCO2 and TCa in blood provided by the present invention;

[0036] Figure 2 This is a schematic diagram of an embodiment of the liquid circuit system of the electrolyte analyzer provided by the present invention.

[0037] Explanation of icon numbers:

[0038] label name label name 100 Electrolyte analyzer liquid circuit system 5 Second detector 1 Sample supply pipeline 51 Electrode box 11 Rotary sampling needle 6 Liquid phase discharge pipeline 12 Sample tubes 61 Rotary cutter valve 2 Diluent supply pipeline 7 Pressure regulating device 21 plunger pump 71 Second pinch valve 3 reaction vessel 8 Mixing pool 31 Sample inlet pipe 91 oil pump 311 First pinch valve 92 peristaltic pump 4 First detector 93 Cleaning fluid supply pipeline 41 pressure sensor 931 Solenoid valve

[0039] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0040] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0041] It should be noted that if the embodiments of the present invention involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.

[0042] Furthermore, if the embodiments of this invention involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this invention.

[0043] Analyzing total carbon dioxide (TCO2) in blood is an important means of understanding alveolar ventilation and gas exchange function, and adding TCO2 detection to electrolyte analyzers has high clinical significance. Current TCO2 detection methods all use pressure methods and directly test blood samples, requiring large sample volumes.

[0044] Therefore, the present invention provides a method for detecting TCO2 and TCa (total calcium concentration) in blood. Figure 1 This is an embodiment of the method for detecting TCO2 and TCa in blood provided by the present invention.

[0045] Please refer to Figure 1 The detection methods for TCO2 and TCa in blood include the following steps:

[0046] Step S10: Provide the sample and diluent.

[0047] It should be noted that the present invention does not limit the specific implementation of the diluent; it can be a mixture of citric acid and Tris (aminobutyric acid), or a mixture of phosphoric acid and Tris. Specifically, in this embodiment, the diluent includes citric acid and Tris.

[0048] The specific preparation steps of the diluent are as follows: Citric acid and Tris are provided and mixed to obtain a preliminary diluent; citric acid is added to the preliminary diluent to make its pH value within the range of 3.2 to 3.6, thus obtaining the final diluent. When the pH value of the diluent is within the range of 3.2 to 3.6, the diluent can react with carbonate ions in the sample to generate carbon dioxide gas, facilitating the detection of TCO2. Simultaneously, the diluent can also complex with calcium ions in the sample, facilitating the detection of calcium ion concentration, ultimately leading to the determination of TCa.

[0049] Step S20: Mix the sample and the diluent to react the sample and the diluent to generate carbon dioxide gas and a mixture containing calcium ion complexes.

[0050] By thoroughly mixing the sample and the diluent, the sample reacts with the diluent to generate carbon dioxide gas and calcium ion complexes.

[0051] Step S30: Detect the carbon dioxide gas content.

[0052] The content of carbon dioxide gas, i.e. the carbon dioxide gas content in the sample, is obtained by detection, and thus the TCO2 in the blood is obtained.

[0053] Step S40: Detect the calcium ion concentration in the mixture to obtain the total calcium concentration.

[0054] After the sample is mixed with the diluent, a calcium ion complex is formed, and the molar ratio of calcium ions to total calcium is kept constant. After detecting the carbon dioxide gas content, the calcium ion concentration in the mixture is directly measured to obtain TCa. This avoids the large errors caused by the previous indirect method of calculating TCa by measuring calcium ions and their pH.

[0055] In the technical solution of the present invention, by mixing the diluent with the sample to generate carbon dioxide gas and calcium ion complex, TCa can be directly tested after TCO2 is detected. Mixing the diluent with the sample is equivalent to diluting the sample once, and a small amount of sample can be used to complete the test, which helps to reduce the amount of sample required for detection. Moreover, no additional reaction process is required between the two tests, which greatly shortens the detection time.

[0056] The present invention also provides a liquid circuit system for an electrolyte analyzer. Figure 2 This is an embodiment of the liquid circuit system of the electrolyte analyzer provided by the present invention. Please refer to... Figure 2The electrolyte analyzer's liquid circuit system 100 includes a sample supply pipe 1, a diluent supply pipe 2, a reaction vessel 3, a first detector 4, and a second detector 5. The diluent supply pipe 2 is used to supply citric acid diluent. The reaction vessel 3 has a sample inlet pipe 31, a gas phase outlet, and a liquid phase outlet. The sample inlet pipe 31 is connected to the sample supply pipe 1 and the diluent supply pipe 2. The first detector 4 is connected to the gas phase outlet and is used to detect the carbon dioxide content in the gas phase. The second detector 5 is connected to the liquid phase outlet and is used to detect the calcium ion concentration in the liquid phase. Both the sample inlet pipe 31 and the liquid phase outlet can be shut off. The working process of the liquid circuit system 100 of the electrolyte analyzer is as follows: First, the sample is added to the reaction container 3 through the sample supply pipe 1, and the citric acid diluent is added to the reaction container 3 through the diluent supply pipe 2. Then, the sample inlet pipe 31 and the liquid phase outlet are closed, and the citric acid diluent and the sample are fully mixed and reacted through the reaction container 3 to generate a mixture of carbon dioxide gas and a calcium ion complex. Then, the content of carbon dioxide gas is detected by the first detector 4 to obtain TCO2. After detection, the liquid phase outlet is opened, and the mixture is discharged to the second detector 5 through the liquid phase outlet. The second detector 5 detects the calcium ion concentration in the mixture to obtain TCa. The liquid circuit system 100 of the electrolyte analyzer realizes the detection of TCO2 and TCa in blood with a small sample volume and a significantly shortened detection time. Furthermore, both the sample inlet pipe 31 and the liquid phase outlet can be closed, so that when carbon dioxide gas is generated during the reaction, both the sample inlet pipe 31 and the liquid phase outlet can be closed to form a sealed space inside the reaction vessel 3, which facilitates detection by the first detector 4 and improves the accuracy of detection.

[0057] It should be noted that the present invention does not limit the specific implementation of the sample inlet pipe 31 and the liquid phase outlet being closable. Specifically, please refer to... Figure 2 In this embodiment, the liquid phase outlet and the second detector 5 are connected through a liquid phase discharge pipe 6. A rotary valve 61 is provided on the liquid phase discharge pipe 6, and a first pinch-off valve 311 is provided on the sample inlet pipe 31. Adjusting the rotary valve 61 controls the opening and closing of the liquid phase discharge pipe 6, thereby opening and closing the liquid phase outlet. The opening and closing of the sample inlet pipe 31 is achieved by controlling the opening and closing of the first pinch-off valve 311. Of course, the sample inlet pipe 31 and the liquid phase outlet can be closed in other ways, such as by providing a solenoid valve 931 on the sample inlet pipe 31 and a solenoid valve 931 on the liquid phase outlet. Other embodiments that allow the sample inlet pipe 31 and the liquid phase outlet to be closed can also be used, and will not be elaborated further here.

[0058] Furthermore, the present invention does not limit the specific implementation of the first detector 4, which can be a pressure sensor 41, etc. Specifically, please refer to... Figure 2 In this embodiment, the first detector 4 includes a pressure sensor 41. When carbon dioxide gas is generated in the reaction vessel 3, the gas pressure in the reaction vessel 3 changes accordingly. The pressure sensor 41 measures the change in gas pressure to obtain TCO2.

[0059] Furthermore, the reaction vessel 3 is also equipped with a pressure regulating device 7 to regulate the gas pressure in the reaction vessel 3. After the TCa test is completed, since the reaction vessel 3 was previously a closed space where air does not circulate easily, the pressure regulating device 7 allows the gas pressure in the reaction vessel 3 to be regulated to ensure air circulation. This ensures that the pressure in the reaction vessel 3 is consistent with the external atmospheric pressure before carbon dioxide gas is generated in the reaction vessel 3 during the next TCO2 test, thus facilitating the next TCO2 test.

[0060] It should be noted that the present invention does not limit the specific implementation of the pressure regulating device 7, which can be a pinch valve, a pressure regulating valve, etc. Specifically, please refer to... Figure 2 In this embodiment, the pressure regulating device 7 is a second pinch-off valve 71. The second pinch-off valve 71 is connected to the reaction vessel 3 and the outside world respectively (initially, the second pinch-off valve 71 is closed, and the reaction vessel 3 is not connected to the outside world). When it is necessary to regulate the gas pressure in the reaction vessel 3, the second pinch-off valve 71 is opened to connect the reaction vessel 3 to the outside world, so that the air in the reaction vessel 3 can be discharged to the outside world, thereby regulating the gas pressure in the reaction vessel 3.

[0061] Furthermore, the present invention does not limit the specific implementation of the second detector 5; it can be any device capable of detecting calcium ion concentration. Specifically, please refer to... Figure 2 In this embodiment, the second detector 5 includes an electrode box 51, which enables high accuracy in detecting calcium ion concentration.

[0062] Please refer to Figure 2 The electrolyte analyzer's liquid path system 100 further includes a mixing chamber 8, which is connected to the sample inlet pipe 31. The mixing chamber 8 is equipped with a sample supply pipe 1 and a diluent supply pipe 2. The mixing chamber 8 facilitates the addition of the sample and the citric acid diluent, improving detection efficiency.

[0063] The electrolyte analyzer's liquid path system 100 also includes a cleaning solution supply device (not shown in the figure) and a peristaltic pump 92. The cleaning solution supply device is used to supply cleaning solution and is connected to the mixing tank 8 via an oil pump 91. The peristaltic pump 92 is located in the sample inlet pipe 31 and between the mixing tank 8 and the reaction vessel 3. The cleaning solution supply device discharges cleaning solution to clean the entire liquid path; the oil pump 91 pumps the cleaning solution discharged by the cleaning solution supply device into the mixing tank 8 to clean the mixing tank 8; the peristaltic pump 92 pumps the cleaning solution, sample, and citric acid diluent from the mixing tank 8 into the reaction vessel 3 and the electrode box 51, stabilizing the flow of the cleaning solution, sample, and citric acid diluent.

[0064] It should be noted that the present invention does not limit the specific implementation of the cleaning fluid supply device, which may be a bucket or tank containing cleaning fluid, etc.

[0065] It should be emphasized that the liquid circuit system 100 of the electrolyte analyzer also includes a cleaning fluid supply pipe 93, which is connected to the mixing tank 8 and the cleaning fluid supply device respectively. The cleaning fluid supply pipe 93 is equipped with a solenoid valve 931 and the oil pump 91.

[0066] Specifically, please refer to Figure 2 In this embodiment, the diluent supply pipe 2 is equipped with a plunger pump 21, and the sample supply pipe 1 includes a rotary sampling needle 11, which is connected to the diluent supply pipe 2, meaning the plunger pump 21 can drive the rotary sampling needle 11 to aspirate. When a sample and citric acid diluent need to be provided, the rotary sampling needle 11 is first rotated to insert into the sample tube 12, and the plunger pump 21 is started to drive the rotary sampling needle 11 to aspirate the sample. After aspiration, the rotary sampling needle 11 is rotated to insert into the mixing chamber 8, and the plunger pump 21 is started again. The plunger pump 21 drives the rotary sampling needle 11 to inject the aspirated sample into the mixing chamber 8, while simultaneously driving the diluent supply pipe 2 to aspirate the citric acid diluent from the diluent reagent bottle (not shown in the figure) into the mixing chamber 8.

[0067] The present invention also provides an electrolyte analyzer, including the liquid circuit system 100 of the electrolyte analyzer described above. The electrolyte analyzer includes all the technical features of the liquid circuit system 100 of the electrolyte analyzer described above, and therefore also has the technical effects brought about by all the above technical features, which will not be described in detail here.

[0068] The above description is merely a preferred embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent transformations made based on the content of the present invention specification and drawings under the concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A method for detecting TCO2 and TCa in blood, characterized in that, Includes the following steps: Provide a sample and a diluent, wherein the pH of the diluent is in the range of 3.2 to 3.6; The sample and the diluent are mixed to cause the sample and the diluent to react and generate carbon dioxide gas and a mixture containing calcium ion complexes; The carbon dioxide gas content was detected; The concentration of calcium ions in the mixture is measured to obtain the total calcium concentration; The preparation steps of the diluent include: providing citric acid and Tris, and mixing the two to obtain a preliminary diluent; Citric acid is added to the preliminary diluent to bring its pH value to the range of 3.2 to 3.6, thus obtaining the diluent. When the pH value of the diluent is in the range of 3.2 to 3.6, the diluent can react with carbonate ions in the sample to generate carbon dioxide gas, which facilitates the detection of TCO2. At the same time, the diluent can also complex with calcium ions in the sample, which facilitates the detection of calcium ion concentration, so as to finally obtain TCa.

2. The method for detecting TCO2 and TCa in blood as described in claim 1, characterized in that, The diluent includes citric acid and Tris.

3. A liquid circuit system for an electrolyte analyzer, characterized in that, include: Sample supply pipeline; A diluent supply pipe is provided for supplying citric acid diluent; The reaction vessel has an inlet pipe, a gas phase outlet, and a liquid phase outlet, wherein the inlet pipe is connected to the sample supply pipe and the diluent supply pipe; The first detector is connected to the gas phase outlet and is used to detect the carbon dioxide content in the gas phase; as well as, The second detector is connected to the liquid phase outlet and is used to detect the concentration of calcium ions in the liquid phase; Both the sample inlet pipe and the liquid phase outlet can be shut off. When the pH value of the diluent is in the range of 3.2 to 3.6, the diluent can react with carbonate ions in the sample to generate carbon dioxide gas, which facilitates the detection of TCO2. At the same time, the diluent can also complex with calcium ions in the sample, which facilitates the detection of calcium ion concentration, so as to finally obtain TCa.

4. The liquid circuit system of the electrolyte analyzer as described in claim 3, characterized in that, The liquid phase outlet and the second detector are connected through a liquid phase discharge pipe; The liquid phase discharge pipe is equipped with a rotary valve, and the sample inlet pipe is equipped with a first pinch valve.

5. The liquid circuit system of the electrolyte analyzer as described in claim 3, characterized in that, The first detector includes a pressure sensor.

6. The liquid circuit system of the electrolyte analyzer as described in claim 5, characterized in that, The reaction vessel is also equipped with a pressure regulating device to regulate the gas pressure in the reaction vessel.

7. The liquid circuit system of the electrolyte analyzer as described in claim 3, characterized in that, The second detector includes an electrode box.

8. The liquid circuit system of the electrolyte analyzer as described in claim 3, characterized in that, It also includes a mixing tank, which is connected to the sample inlet pipe, and the mixing tank is provided with the sample supply pipe and the diluent supply pipe.

9. The liquid circuit system of the electrolyte analyzer as described in claim 8, characterized in that, Also includes: A cleaning fluid supply device for supplying cleaning fluid, the cleaning fluid supply device being connected to the mixing tank via an oil pump; and... A peristaltic pump is installed in the sample inlet pipe and located between the mixing tank and the reaction vessel.

10. An electrolyte analyzer, characterized in that, Includes the liquid circuit system of the electrolyte analyzer as described in any one of claims 3 to 9.

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