Cell staining instrument

Abstract

The present invention discloses a cell staining instrument, which comprises a frame, a glass slide swinging assembly, a reagent chamber, and an injection assembly. The glass slide swinging assembly, the reagent chamber, and the injection assembly are all mounted on the frame, and the injection assembly is movably mounted above the glass slide swinging assembly and the reagent chamber. The glass slide swinging assembly comprises a base and a glass slide carrier, and the glass slide carrier is swingably mounted on the base. A swinging motor and an arc guide rail are provided on the base, which can realize stable swinging of the glass slide carrier and ensure uniform staining of cells. The reagent chamber comprises a reagent chamber fixing frame and a reagent rack mounted on the reagent chamber fixing frame. The reagent chamber can realize the fixation of reagent tubes and ensure that the injection assembly absorbs reagents. The cell staining instrument of the present invention can effectively ensure the stable swinging of the glass slide, and the cells can be uniformly stained. The reagent rack is stably installed, and has the advantages of simple structure, high stability of the reagent rack, and high reliability.

Description

Technical Field

[0001] The invention relates to cell staining equipment, in particular to a cell staining instrument. Background Art

[0002] Conventional staining machines can only stain tissue cells because the distribution densities of tissue cells and pure cells on tissue slides are different. Tissue cells are water-absorbent, and the liquid will be absorbed and enriched around the tissue after being dripped. The relative density of pure cells is dispersed, and the liquid will not gather around the cells. It is easy to disperse and flow away, resulting in unstable staining results and poor uniformity. The present invention solves the problem of cell staining stability. A rocking mechanism is used to allow the liquid to flow repeatedly in the cell area of ​​the slide, thereby achieving a mixing effect. At the same time, the liquid will not flow away quickly due to the poor water absorption of the cells. Different tilt angles are used in the antibody addition and washing steps to change the fluidity of the liquid, so that the cells are continuously covered by the liquid, and the reaction time is more sufficient when kept horizontal. When washing, the tilt angle is increased, the liquid flows away quickly, the slide is rinsed cleaner, and the staining background is clearer. Summary of the invention

[0003] The technical problem to be solved by the present invention is to provide a cell staining instrument to address the problem that cells in the cell staining instrument cannot be well stained.

[0004] The present invention achieves the above-mentioned object through the following technical scheme: a cell staining instrument, which includes a frame, a glass slide swing assembly, a reagent compartment, and an injection assembly, wherein the glass slide swing assembly, the reagent compartment, and the injection assembly are all installed on the frame, and the injection assembly is movably installed above the glass slide swing assembly and the reagent compartment;

[0005] The slide swing assembly includes a base and a slide stage, and the slide stage is swingably mounted on the base;

[0006] The base is composed of a structural fixing plate, a front fixing frame, a rear fixing frame, and a swing motor. The front fixing frame and the rear fixing frame are mounted on the structural fixing plate. The swing motor is mounted on the front fixing frame or the rear fixing frame, and an arc guide rail is provided on the front fixing frame or the rear fixing frame relative to the swing motor. The glass slide stage is arranged between the front fixing frame and the rear fixing frame. One end of the glass slide stage is connected to the swing motor, and the other end is mounted on the arc guide rail.

[0007] The reagent bin includes a reagent bin fixing frame and a reagent rack installed on the reagent bin fixing frame;

[0008] The reagent bin fixing frame includes a fixing frame body, a guide slide bar, a fixing plate, and an induction recognition circuit board. A plurality of guide slide bars are arranged in parallel on the fixing frame body, the fixing plate is arranged at one end of the guide slide bar, and the induction recognition circuit board is arranged above the guide slide bar.

[0009] The reagent rack is provided with an identification chip and a reagent rack slide, wherein the identification chip is arranged on the top surface of the reagent rack, and the reagent rack slide is arranged on the end surface of one end of the reagent rack;

[0010] The fixed plate is provided with a slide socket matched with the slide of the reagent rack, and the thickness of the middle part of the slide of the reagent rack is greater than the thickness of the two ends, presenting a spindle-shaped structure.

[0011] As a further optimization solution of the present invention, a reagent rack locking piece is further provided on the reagent rack, and the reagent rack locking piece is located below the reagent rack sliding piece.

[0012] As a further optimization scheme of the present invention, a through-plate hole is opened on the fixed plate, the through-plate hole is located at the lower side of the slide socket, and the reagent rack locking piece passes through the through-plate hole.

[0013] As a further optimization solution of the present invention, the reagent bin fixing rack further includes a reagent rack fixing device, which is mounted on the outer side surface of the fixing plate and cooperates with the reagent rack locking piece.

[0014] As a further optimization solution of the present invention, the reagent rack holder is a solenoid valve.

[0015] As a further optimization solution of the present invention, the swing angle of the slide stage is 5°-20°.

[0016] As a further optimization solution of the present invention, the time for the glass slide stage to swing for one cycle is one minute.

[0017] As a further optimization solution of the present invention, the swing motor is a stepping motor.

[0018] As a further optimization scheme of the present invention, the injection assembly consists of an injection pump, a mechanical arm, and an injection needle. The mechanical arm is slidably mounted on the frame, the injection needle is slidably mounted on the mechanical arm, and the injection pump is connected to the injection needle.

[0019] As a further optimization solution of the present invention, the cell staining instrument also includes a cleaning and mixing station, which is located below the injection component.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] By setting up an induction recognition circuit board and an recognition chip, the source of the goods can be identified.

[0022] By providing a reagent rack slide with a spindle-shaped structure, it is ensured that when the reagent rack slide is pushed into the slide socket, the reagent rack slide can be in place and the tail of the reagent rack slide can be locked in the slide socket.

[0023] By providing a reagent rack fixer and a reagent rack locking piece, it is further ensured that the reagent rack is fixed on the reagent compartment fixing rack.

[0024] By setting a swing motor and an arc guide rail, the slide stage can be ensured to swing stably. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the structure of a cell staining instrument of the present invention;

[0026] Figure 2 This is a front view of a cell staining instrument of the present invention;

[0027] Figure 3 This is a rear view of a cell staining instrument of the present invention;

[0028] Figure 4 This is a schematic diagram of the structure of a slide swing assembly of a cell staining instrument of the present invention;

[0029] Figure 5 It is a front view of a slide swing assembly of a cell staining instrument of the present invention;

[0030] Figure 6 It is a schematic diagram of the arc guide rail structure of a slide swing assembly of a cell staining instrument of the present invention;

[0031] Figure 7 It is a schematic diagram of the structure of a reagent chamber of a cell staining instrument of the present invention;

[0032] Figure 8 This is a front view of a reagent compartment of a cell staining instrument of the present invention;

[0033] Fig. 9 It is a top view of a reagent compartment of a cell staining instrument of the present invention;

[0034] Fig.10 It is a side view of a reagent compartment of a cell staining instrument of the present invention;

[0035] Fig.11 It is a schematic diagram of the structure of a reagent rack of a cell staining instrument of the present invention;

[0036] Fig.12 It is a structural schematic diagram of a fixed plate of a cell staining instrument of the present invention;

[0037] Fig.13 This is a schematic structural diagram of a cell staining instrument embodiment 2 of the present invention;

[0038] 1-frame, 2-slide swing assembly, 3-reagent chamber, 4-injection assembly, 5-cleaning and mixing station, 21-base, 22-slide carrier, 23-arc guide rail, 24-reagent rack locking plate, 211-structure fixing plate, 212-front fixing frame, 213-rear fixing frame, 214-swing motor, 31-reagent chamber fixing frame, 32-reagent rack, 311-fixed frame body, 312-guide slide, 313-fixed plate, 314-induction recognition circuit board, 315-reagent rack holder, 321-identification chip, 322-reagent rack slide, 3131-slide socket, 3132-through-plate hole, 41-injection pump, 42-mechanical arm, 43-injection needle. DETAILED DESCRIPTION

[0039] The present application is further described in detail below in conjunction with the accompanying drawings. It is necessary to point out here that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technical personnel in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0040] Example 1

[0041] like Figure 1 , Figure 2 The cell staining instrument shown includes a frame 1, a slide swing assembly 2, a reagent chamber 3, and an injection assembly 4. The slide swing assembly 2, the reagent chamber 3, and the injection assembly 4 are all mounted on the frame 1, and the injection assembly 4 is movably mounted above the slide swing assembly 2 and the reagent chamber 3. The reagent in the reagent chamber 3 is extracted by the injection assembly 4 and then dripped into the slide swing assembly 2, and the slide swing assembly 2 is stably swung to achieve staining.

[0042] like Figure 4 , Figure 5 The slide rocking assembly 2 shown includes a base 21 and a slide carrier 22 , and the slide carrier 22 is swingably mounted on the base 21 .

[0043] like Figure 5 , Figure 6As shown, the base 21 is composed of a structural fixing plate 211, a front fixing frame 212, a rear fixing frame 213, and a swing motor 214. The front fixing frame 212 and the rear fixing frame 213 are installed on the structural fixing plate 211. The swing motor 214 is installed on the front fixing frame 212 or the rear fixing frame 213, and an arc guide rail 23 is provided on the front fixing frame 212 or the rear fixing frame 213 relative to the swing motor 214. The glass slide stage 22 is arranged between the front fixing frame 212 and the rear fixing frame 213. One end of the glass slide stage 22 is connected to the swing motor 214, and the other end is installed on the arc guide rail 23. The swing motor uses a stepping motor. Compared with other swinging modes, its swing is more stable and the staining is more uniform. The glass slide stage 22 can be driven by the swing motor 214 to realize slow swinging on the arc guide rail 23. When swinging, the swing angle of the glass slide stage 22 is 5°-20°, and the swing cycle is 1-2 minutes.

[0044] like Figure 7 As shown, the reagent chamber 3 includes a reagent chamber fixing frame 31 and a reagent rack 32 installed on the reagent chamber fixing frame 31 .

[0045] like Figure 8 , Fig. 9 , Fig.10 As shown, the reagent compartment fixing frame 31 includes a fixing frame body 311, a guide slide 312, a fixing plate 313, and an induction identification circuit board 314. A plurality of guide slides 312 are arranged in parallel on the fixing frame body 311. A slide groove matching the guide slide 312 is arranged at the bottom of the reagent rack 32. The guide slide 312 can guide the reagent rack 32 to be fixed to the reagent compartment fixing frame 31. The fixing plate 313 is arranged at one end of the guide slide 312, and its function is to further fix the reagent rack 32. A slide socket 3131 matching the reagent rack slide 322 is provided on the fixing plate 313. When in use, one end of the reagent rack 32 can be inserted into the slide socket 3131 for fixing. The induction identification circuit board 314 is arranged above the guide slide bar 312. The induction identification circuit board 314 is the key to identifying the reagent rack 32 and can be used to sense the RFID chip installed on the reagent rack 32. When the induction identification circuit board 314 senses that the reagent rack 32 is inserted, it will read the information of the reagent rack 32, and the rack information will be transmitted to the corresponding device.

[0046] like Fig.11As shown, the reagent rack 32 is provided with an identification chip 321 and a reagent rack slide 322. The identification chip 321 is arranged on the top surface of the reagent rack 32, and the reagent rack slide 322 is arranged on the end surface of one end of the reagent rack 32. The reagent rack slide 322 matches the slide socket 3131, and the middle thickness of the reagent rack slide 322 is greater than the thickness at both ends, presenting a spindle-shaped structure. When in use, when the reagent rack slide 322 is inserted into the slide socket 3131, the reagent rack slide 322 will cause the slide socket 3131 and its internal spring to open a certain angle. After entering, the tail of the slide socket 3131 will be locked. In this process, the inserter will have a feeling of loading in place.

[0047] like Fig.12 As shown, the fixing plate 313 is also provided with a reagent rack fixture 315 and a through-plate hole 3132, and the reagent rack 32 is also provided with a reagent rack locking piece 24. The reagent rack fixture 315 and the through-plate hole 3132 cooperate with the reagent rack locking piece 24, and the reagent rack fixture 315 uses a solenoid valve. When the position of the reagent rack 32 is no longer moved, after the induction recognition circuit board 314 senses the recognition chip 321, the solenoid valve works, and the iron core of the solenoid valve moves upward and passes through the locking hole on the reagent rack locking piece 24, so that the reagent rack can no longer be pulled out.

[0048] The injection assembly 4 is composed of a syringe pump 41, a mechanical arm 42, and an injection needle 43. The mechanical arm 42 is slidably mounted on the frame 1, and the injection needle 43 is slidably mounted on the mechanical arm 42. The syringe pump 41 is connected to the injection needle 43. During operation, the mechanical arm 42 slides on the frame 1 and can be adjusted to above the liquid aspiration position, and then the injection needle 43 slides longitudinally on the mechanical arm 42, and the injection needle 43 slides to just above the aspiration position to aspirate liquid.

[0049] The cleaning and mixing station 5 is located below the injection assembly 4 and can be used to clean the injection needle 43. Since the absorbed reagents are different, after the injection needle absorbs the reagents, it will enter the cleaning and mixing station 5 for cleaning.

[0050] like Figure 3 As shown, the cell staining instrument of the present invention also includes a control circuit board 5, a fan 6, a power supply 7, a large-capacity bottle 8, and a housing 9. The control circuit board 5 is used to control and coordinate the operation of the entire machine, the large-capacity bottle 8 is used to store reagents, and a button for controlling the operation of the cell staining instrument is provided on the housing 9, which can realize the operation of the cell staining instrument.

[0051] When working, first put the reagent tubes in batches on the reagent rack, and then put the reagent rack into the reagent compartment fixing rack. The reagent compartment fixing rack identifies the reagent rack. After the identification is correct, the injection assembly starts to work, and the injection needle 43 absorbs the reagent and drips it onto the glass slide swing assembly. The motor of the glass slide swing assembly 2 starts to work, driving the glass slide stage to swing. After a certain swing time, the staining is completed. At the same time, the injection assembly controls the injection needle to enter the cleaning and mixing station for cleaning, waiting for the next dripping.

[0052] Example 2

[0053] like Fig.13 As shown, the glass slide swing assembly 2 can adopt a cylinder swing mode, and one cylinder can be fixed on the glass slide stage 22 at a time, and the glass slide stage 22 can be driven to swing back and forth by the extension and contraction of the cylinder.

[0054] The above-described embodiments are merely descriptions of preferred implementations of the present invention, and are not intended to limit the concept and scope of the present invention. Without departing from the design concept of the present invention, various modifications and improvements made by ordinary persons in the art to the technical solution of the present invention should fall within the protection scope of the present invention, and the technical contents for which protection is sought in the present invention have been fully recorded in the claims.

Claims

1. A cell staining instrument, characterized in that: It comprises a frame (1), a glass slide swing assembly (2), a reagent compartment (3), and an injection assembly (4); the glass slide swing assembly (2), the reagent compartment (3), and the injection assembly (4) are all mounted on the frame (1); and the injection assembly (4) is movably mounted above the glass slide swing assembly (2) and the reagent compartment (3); The glass slide swing assembly (2) comprises a base (21) and a glass slide carrier (22), wherein the glass slide carrier (22) is swingably mounted on the base (21); The base (21) is composed of a structural fixing plate (211), a front fixing frame (212), a rear fixing frame (213), and a swing motor (214); the front fixing frame (212) and the rear fixing frame (213) are mounted on the structural fixing plate (211); the swing motor (214) is mounted on the front fixing frame (212) or the rear fixing frame (213); an arc guide rail (23) is provided on the front fixing frame (212) or the rear fixing frame (213) relative to the swing motor (214); the glass slide stage (22) is arranged between the front fixing frame (212) and the rear fixing frame (213); one end of the glass slide stage (22) is connected to the swing motor (214), and the other end is mounted on the arc guide rail (23); The reagent chamber (3) comprises a reagent chamber fixing frame (31) and a reagent rack (32) mounted on the reagent chamber fixing frame (31); The reagent bin fixing frame (31) comprises a fixing frame body (311), a guide slide bar (312), a fixing plate (313), and an inductive identification circuit board (314); a plurality of guide slide bars (312) are arranged in parallel on the fixing frame body (311); the fixing plate (313) is arranged at one end of the guide slide bar (312); and the inductive identification circuit board (314) is arranged above the guide slide bar (312); The reagent rack (32) is provided with an identification chip (321) and a reagent rack slide (322), wherein the identification chip (321) is arranged on the top surface of the reagent rack (32), and the reagent rack slide (322) is arranged on the end surface of one end of the reagent rack (32); The fixed plate (313) is provided with a slide socket (3131) matched with the reagent rack slide (322), and the thickness of the middle part of the reagent rack slide (322) is greater than the thickness of the two ends, presenting a spindle-shaped structure.

2. The cell staining instrument according to claim 1, characterized in that: The reagent rack (32) is also provided with a reagent rack locking piece (24), and the reagent rack locking piece (24) is located below the reagent rack sliding piece (322).

3. The cell staining instrument according to claim 1, characterized in that: A through-plate hole (3132) is provided on the fixing plate (313), and the through-plate hole (3132) is located at the lower side of the slide socket (3131), and the reagent rack locking piece (24) passes through the through-plate hole (3132).

4. The cell staining instrument according to claim 1, characterized in that: The reagent bin fixing frame (31) further comprises a reagent frame fixing device (315), which is mounted on the outer side of the fixing plate (313), and the reagent frame fixing device (315) cooperates with the reagent frame locking piece (24).

5. The cell staining instrument according to claim 4, characterized in that: The reagent rack holder (315) is a solenoid valve.

6. The cell staining instrument according to claim 1, characterized in that: The swing angle of the glass slide stage (22) is 5°-20°.

7. The cell staining instrument according to claim 1, characterized in that: The time for the glass slide stage (22) to rock for one cycle is one minute.

8. The cell staining instrument according to claim 1, characterized in that: The swing motor (214) is a stepping motor.

9. The cell staining instrument according to claim 1, characterized in that: The injection assembly (4) is composed of an injection pump (41), a mechanical arm (42), and an injection needle (43). The mechanical arm (42) is slidably mounted on the frame (1), the injection needle (43) is slidably mounted on the mechanical arm (42), and the injection pump (41) is connected to the injection needle (43).

10. The cell staining instrument according to claim 1, characterized in that: The cell staining instrument further comprises a cleaning and mixing station (5), and the cleaning and mixing station (5) is located below the injection assembly (4).

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