Slide preparation and staining device

Abstract

The present invention relates to a slide preparation and staining device, comprising a workbench, a rotating disk, a moving part and a suction nozzle driving part. The rotating disk is arranged on the workbench, and a plurality of centrifuge tubes are arranged in a ring on the rotating disk. Staining chambers are arranged on the rotating disk at intervals corresponding to the centrifuge tubes. The moving part is arranged at intervals on one side of the rotating disk, and a recovery box and a suction nozzle box are arranged on the moving part. The suction nozzle driving part comprises a crossbeam arranged above the workbench, and a mechanical arm that can move back and forth along a second direction is slidably arranged on the crossbeam. The mechanical arm has a first position and a second position. When the mechanical arm is in the first position, the mechanical arm moves to a working position to grab the suction nozzle or eject the used suction nozzle. When the mechanical arm is in the second position, the mechanical arm transfers the cells in the centrifuge tube to the staining chamber. The slide preparation and staining device of the present invention can replace the entire box of suction nozzles when the suction nozzles in the suction nozzle box are used up. The operation is simple and the working efficiency is high. The automation and integration degree of the slide preparation and staining device are high.

Description

Technical Field

[0001] The present invention relates to the technical field of cell detection, in particular to a slide preparation and staining device. Background Art

[0002] Cells are the basic structural and functional units of the human body. By observing changes and abnormalities in cells across various tissues, we can determine whether a person has pathological changes. Cell preparation is key to cytopathology testing. For example, the most common method for diagnosing cervical lesions in women is to obtain a smear of exfoliated cells, fix them, stain them, and then examine them under a microscope.

[0003] Currently, the working principle of the cell preparation and staining device is to use a controller to control the robotic arm to transfer the cell sample in the centrifuge tube to the corresponding staining chamber or glass slide for preparation and staining. The preparation and staining amount in each workflow is small. After the cells in each centrifuge tube are transferred, the used nozzle is manually removed and replaced with a new nozzle for the next transfer. The operation is frequent and time-consuming. Summary of the Invention

[0004] The purpose of the embodiment of the present invention is to provide a slide making and dyeing device with a simple structure and high working efficiency.

[0005] To achieve this purpose, the embodiment of the present invention adopts the following technical solutions:

[0006] Provided is a slide making and dyeing device, comprising:

[0007] Workbench;

[0008] A rotating disk, the rotating disk is arranged on the workbench, a plurality of centrifuge tubes are arranged in an annular manner on the rotating disk, and dyeing chambers are arranged on the rotating disk corresponding to the centrifuge tubes at intervals;

[0009] a moving portion, the moving portion being spaced apart and arranged on one side of the rotating disk, the moving portion being provided with a recovery box and a nozzle box, the moving portion being capable of driving the recovery box and the nozzle box to reciprocate along a first direction so that the recovery box and the nozzle box are alternately located at a working position;

[0010] The nozzle driving member includes a crossbeam arranged above the workbench, and a robotic arm that can move back and forth along the second direction is slidably arranged on the crossbeam. The robotic arm has a first position and a second position. When the robotic arm is located at the first position, the robotic arm moves to the working position to grab the nozzle or eject the used nozzle. When the robotic arm is located at the second position, the robotic arm transfers the cells in the centrifuge tube to the staining chamber.

[0011] As a preferred solution of the film making and dyeing device, the rotating disk includes a first turntable and a second turntable, the first turntable and the second turntable are arranged at intervals on the workbench, and the robotic arm includes a first robotic arm arranged corresponding to the first turntable and a second robotic arm arranged corresponding to the second turntable.

[0012] As a preferred solution of the film making and dyeing device, a slide is provided on the workbench, the slide is located between the first turntable and the second turntable, and the moving part is provided in the slide.

[0013] As a preferred solution of the film making and dyeing device, the moving part includes a moving platform, and a first mounting position and a second mounting position are arranged on the moving platform along the moving direction of the moving platform. The recovery box is arranged in the first mounting position, and the suction nozzle box is arranged in the second mounting position. One of the first mounting position and the second mounting position is located in the working position.

[0014] As a preferred solution for the film making and dyeing device, at least two baffles are provided on the workbench and at the working position, and the two baffles are respectively provided on opposite sides of the slide groove. When the nozzle box moves to the working position, the baffles press the nozzle box to prevent the nozzle box from detaching from the moving part when the robotic arm takes out the nozzle.

[0015] As a preferred solution of the slide dyeing device, a boss is provided at one end of the movable platform, a first mounting groove for mounting a dye bottle is provided on the boss, and an observation position is provided on the side wall of the boss corresponding to the first mounting groove; or,

[0016] A boss is provided at one end of the movable platform, a first mounting groove for mounting a dye bottle is provided on the boss, and a weight sensor is provided at the bottom of the first mounting groove.

[0017] As a preferred solution of the slide preparation and staining device, it includes a rotating arm, which includes a rotating shaft and an arm body. The rotating shaft is rotatably set on the workbench, one end of the rotating shaft is connected to the arm body, and a liquid adding tube is provided on a side of the arm body close to the workbench. A reagent bottle is provided on the workbench, and the liquid adding tube is connected to the reagent bottle. The liquid adding tube can add the reagent in the reagent bottle to the centrifuge tube and the staining chamber.

[0018] As a preferred solution of the slide preparation and staining device, a weight sensor is provided on the workbench, and the reagent bottle is provided on the weight sensor.

[0019] As a preferred solution for the slide preparation and staining device, an emptying groove is provided on the workbench, and the emptying groove is spaced apart from the rotating shaft. The arm body can rotate to the top of the emptying groove and can empty the residual reagent in the liquid filling tube into the emptying groove.

[0020] As a preferred solution of the slide making and dyeing device, a mounting plate is provided on the rotating disk, the mounting plate is located between the centrifuge tube and the peripheral side of the rotating disk, and fixing parts are convexly provided on opposite sides of the mounting plate, and the fixing parts are provided with fixing grooves on the side walls, and the notches of the fixing grooves face the center of the mounting plate, and the upper end of the fixing part is provided with a mounting port, the mounting port is communicated with the fixing groove, and the groove wall of the fixing groove is concavely provided with a clamping groove;

[0021] The dyeing chamber includes a main body and two fixing parts, which are arranged on the outer side wall of the main body relative to each other. The fixing parts are inserted into the fixing groove through the installation opening and rotated at a set angle to be clamped in the clamping groove.

[0022] The beneficial effects of the embodiments of the present invention are as follows: a moving part is arranged on the workbench, a recovery box and a nozzle box are arranged on the moving part, the moving part can drive the recovery box and the nozzle box to reciprocate along the first direction, a nozzle driving member is arranged above the workbench, the nozzle driving member includes a beam arranged above the workbench, a mechanical arm that can reciprocate along the second direction is slidably arranged on the beam, the mechanical arm has a first position and a second position, so that when the mechanical arm is in the first position, the mechanical arm moves to the working position to grab the nozzle or withdraw the used nozzle, and when the mechanical arm is in the second position, the mechanical arm transfers the cells in the centrifuge tube to the staining chamber, and when the nozzles in the nozzle box are used up, the whole box can be replaced, the operation is simple, the work efficiency is high, and the degree of automation and integration of the slide preparation and staining device is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The present invention will be described in further detail below with reference to the accompanying drawings and examples.

[0024] Figure 1 This is a first-view stereoscopic schematic diagram of a slide making and dyeing device according to an embodiment of the present invention.

[0025] Figure 2 for Figure 1 A partial enlarged view of point A.

[0026] Figure 3 for Figure 1 A partial enlarged view of point B.

[0027] Figure 4 This is a schematic diagram of a second perspective perspective of the slide making and dyeing device according to an embodiment of the present invention.

[0028] Figure 5 for Figure 4 A partial enlarged view of point C.

[0029] Figure 6 2 is a structural diagram of a mobile station according to an embodiment of the present invention.

[0030] Figure 7 Schematic diagram of the structure of the rotating arm according to an embodiment of the present invention.

[0031] Figure 8 Schematic diagram of the exploded view of the nozzle box according to an embodiment of the present invention.

[0032] Figure 9 Schematic diagram of the structure of the nozzle tray according to an embodiment of the present invention.

[0033] Figure 10 Schematic diagram of the structure of the box body according to an embodiment of the present invention.

[0034] In the picture:

[0035] 1. Workbench; 11. Baffle; 2. Rotating disk; 21. Centrifuge tube; 22. Staining chamber; 221. Main body; 222. Fixing member; 23. First turntable; 24. Second turntable; 25. Mounting plate; 26. Fixing portion; 261. Mounting opening; 27. Channel; 3. Moving portion; 31. Moving table; 311. Limiting block; 312. Partition; 313. Boss; 3131. First mounting slot; 3132. Observation position; 32. Recovery box; 33. Nozzle box; 331. Box body; 3311. First supporting portion; 33111. First cavity; 3312. Second supporting portion; 33121. First supporting plate; 33122. Second supporting plate; 33123. Second connecting plate; 3313, mounting portion; 33131, first limiting member; 33132, second limiting member; 3314, protrusion; 3315, anti-slip groove; 332, nozzle tray; 3321, through hole; 3322, first reinforcing beam; 3323, second reinforcing beam; 3324, buckle; 3325, second cavity; 4, nozzle driving member; 41, crossbeam; 42, robotic arm; 421, first robotic arm; 422, second robotic arm; 5, rotating arm; 51, rotating shaft; 52, arm body; 521, liquid adding tube; 5211, first buffer tube; 5212, second buffer tube; 5213, alcohol tube; 6, drain tank; 7, reagent bottle; 8, touch screen. DETAILED DESCRIPTION

[0036] To make the technical problems solved, the technical solutions adopted, and the technical effects achieved by the present invention more clearly understood, the technical solutions of the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings. It is apparent that the described embodiments are only some of the embodiments of the present invention, not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort shall fall within the scope of protection of the present invention.

[0037] In the description of the present invention, unless otherwise expressly specified or limited, the terms "connected," "connected," and "fixed" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention in specific circumstances.

[0038] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Furthermore, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0039] Cells are the basic structural and functional units of the human body. By observing changes and abnormalities in cells across various tissues, we can determine whether a person has pathological changes. Cell staining can also be used to diagnose pathological changes. The following describes the slide preparation and staining device of the present invention in detail using specific examples.

[0040] like Figures 1 to 5As shown, the slide preparation and staining device of the present invention includes a workbench 1, a rotating disk 2, a moving part 3 and a nozzle driving member 4, the rotating disk 2 is arranged on the workbench 1, and a plurality of centrifuge tubes 21 are arranged in an annular manner on the rotating disk 2. Staining chambers 22 are arranged at intervals corresponding to the centrifuge tubes 21 on the rotating disk 2, the moving part 3 is arranged at intervals on one side of the rotating disk 2, and a recovery box 32 and a nozzle box 33 are provided on the moving part 3. The moving part 3 can drive the recovery box 32 and the nozzle box 33 to move back and forth along a first direction so that the recovery box 32 and the nozzle box 33 are alternately located in a working position. The nozzle driving member 4 includes a crossbeam 41 arranged above the workbench 1, and a mechanical arm 42 that can move back and forth along a second direction is slidably provided on the crossbeam 41. The mechanical arm 42 has a first position and a second position. When the mechanical arm 42 is in the first position, the mechanical arm 42 moves to the working position to grab the nozzle or withdraw the used nozzle. When the mechanical arm 42 is in the second position, the mechanical arm 42 transfers the cells in the centrifuge tube 21 to the staining chamber 22. It is understandable that the slide dyeing device further comprises a frame, a workbench 1 is arranged on the frame, a rotating disk 2 is provided on the workbench 1, a plurality of centrifuge tubes 21 are arranged in a ring on the rotating disk 2, a dyeing chamber 22 is provided on the rotating disk 2 corresponding to the centrifuge tubes 21, the number of the dyeing chamber 22 is equal to the number of the centrifuge tubes 21, and the rotating disk 2 can drive the centrifuge tubes 21 and the dyeing chamber 22 to rotate together. A moving part 3 is provided on the workbench 1, the moving part 3 is spaced apart on one side of the rotating disk 2, a recovery box 32 and a nozzle box 33 are provided on the moving part 3, the nozzle box 33 stores nozzles, and after the nozzles stored in the nozzle box 33 are used up, the nozzle box 33 in which the nozzles are stored in advance can be directly replaced as a whole, which is simple to operate and does not affect the operation of the slide dyeing device. The moving part 3 can drive the recovery box 32 and the nozzle box 33 to move back and forth along the first direction. During the movement, the recovery box 32 and the nozzle box 33 will alternately change their working positions. The nozzle driving member 4 includes a crossbeam 41, which is arranged on the frame and above the workbench 1. A robotic arm 42 that can move back and forth in the second direction is provided on the crossbeam 41. When the robotic arm 42 moves to the first position, it removes the nozzle or grabs the nozzle. After the robotic arm 42 grabs the nozzle, the robotic arm 42 moves to the second position along the crossbeam 41. The robotic arm 42 transfers the cells in the centrifuge tube 21 to the staining chamber 22 through the nozzle. After the transfer is completed, the robotic arm 42 moves back to the first position along the crossbeam 41 to remove the used nozzle from the robotic arm 42. The entire working process of the slide preparation and staining device requires less human intervention. The installation and disassembly of the nozzle are both completed independently by the robotic arm 42, and the mutual cooperation between the moving part 3 and the robotic arm 42 has a high degree of automation and integration.

[0041] It should be noted that if Figure 1 and Figure 4As shown, the first direction in this embodiment is the Y-axis direction shown by the coordinate axis, the second direction is the X-axis direction shown by the coordinate axis, and the direction in which the robot arm 42 moves along the vertical direction is the Z-axis direction shown by the coordinate axis.

[0042] As a preferred solution, 16 centrifuge tubes 21 are arranged on the rotating disk 2. Similarly, 16 dyeing chambers 22 are arranged on the rotating disk 2 corresponding to the centrifuge tubes 21, so that the rotating disk 2 can drive the 16 centrifuge tubes 21 and the 16 dyeing chambers 22 to rotate at the same time, thereby improving the working efficiency of the film making and dyeing device.

[0043] Specifically, the rotating disk 2 includes a first turntable 23 and a second turntable 24 , which are spaced apart on the workbench 1 , and the robotic arm 42 includes a first robotic arm 421 corresponding to the first turntable 23 and a second robotic arm 422 corresponding to the second turntable 24 . In this embodiment, the first turntable 23 and the second turntable 24 have the same shape and size, and the number and position of the centrifuge tubes 21 and staining chambers 22 arranged on the first turntable 23 and the second turntable 24 are also the same. The first robotic arm 421 operates independently corresponding to the first turntable 23, and the second robotic arm 422 operates independently corresponding to the second turntable 24. The two do not affect each other. The two robotic arms 42 can share the recovery box 32 and the nozzle box 33 provided on the moving part 3. When the preparation and staining device is operating normally, 32 staining chambers 22 can be used for cell staining at the same time. If one of the rotating disks 2 or the robotic arm 42 fails, it will not affect the operation of the other rotating disk 2 or the robotic arm 42 of the device. On the basis of greatly improving work efficiency, it can also prevent the inability to perform staining due to a failure of a rotating disk 2 or the robotic arm 42.

[0044] In another embodiment, the rotating disk 2 includes a first rotating disk 23 and a second rotating disk 24. Only one robotic arm 42 is arranged on the beam 41, and the first rotating disk 23 and the second rotating disk 24 share one robotic arm 42. The robotic arm 42 can move back and forth along the beam 41 toward the first rotating disk 23 and the second rotating disk 24. When one of the rotating disks 2 fails, the failed rotating disk 2 can be closed, and the robotic arm 42 can work on the other rotating disk 2 alone, which can also prevent the dyeing work from being unable to be carried out due to the failure of the rotating disk 2.

[0045] Of course, the number of turntables is not limited to one or two, but can also be three, four or more. It can be designed according to specific needs. An independent robotic arm 42 can be set for each turntable, and multiple turntables can be set. The multiple turntables can be operated separately and independently, or simultaneously or staggered, which increases the carrying capacity and meets the needs of the experiment more flexibly.

[0046] In one embodiment, a slide groove is provided on the workbench 1, and the slide groove is located between the first turntable 23 and the second turntable 24. The moving part 3 is arranged in the slide groove. The moving part 3 can move back and forth in the first direction in the slide groove, and the moving part 3 is arranged in the slide groove so that the protruding part of the moving part 3 relative to the surface of the workbench 1 is not too high, which is more beautiful in structure. The movement range of the robotic arm 42 in the vertical direction does not need to be too high, which can improve the working efficiency of the robotic arm 42. The distance between the moving part 3 and the first turntable 23 and the second turntable 24 is equal, and the first robotic arm 421 and the second robotic arm 422 are easier to coordinate with the moving part 3.

[0047] Specifically, the moving part 3 includes a moving platform 31, and a first installation position and a second installation position are set on the moving platform 31 along the moving direction of the moving platform 31. The recovery box 32 is set in the first installation position, and the nozzle box 33 is set in the second installation position. One of the first installation position and the second installation position is located in the working position. In this embodiment, Figure 1 、 Figure 2 and Figure 6 As shown, the movable platform 31 is provided with a mounting position, and at least four limiting blocks 311 are provided around the mounting position. The four limiting blocks 311 are distributed at the four corners of a rectangle. A partition 312 is provided between the four limiting blocks 311. The partition 312 divides the mounting position into a first mounting position and a second mounting position of equal size. The limiting blocks 311 limit the position of the nozzle box 33 and the recovery box 32. When the movable portion 3 moves along the direction of the movable platform 31, the nozzle box 33 and the recovery box 32 will not shift in position. In other embodiments, two mounting grooves are recessed on the movable platform 31, and the nozzle box 33 and the recovery box 32 can both be installed in the mounting grooves.

[0048] As a preferred solution, the limit block 311 structure is "L"-shaped, and the limit part includes a first limit plate and a second limit plate. The first limit plate and the second limit plate are set at an angle, and the angle directions of the four limit blocks 311 are all toward the center of the installation position. The first limit plate and the second limit plate set at an angle limit the suction nozzle box 33 and the recovery box 32 in the first direction and the second direction, so that the suction nozzle box 33 and the recovery box 32 are more stable during the movement.

[0049] As a preferred solution, anti-counterfeiting detectors are installed at the bottom of the first and second mounting positions, and anti-counterfeiting codes are provided on the bottoms of the nozzle box 33 and the recovery box 32. When the nozzle box 33 and the recovery box 32 are installed in the first and second mounting positions, the anti-counterfeiting detectors detect the anti-counterfeiting codes. If the anti-counterfeiting detectors fail the detection, the slide preparation and dyeing device stops operating; otherwise, it continues operating. This effectively prevents operators from using counterfeit nozzle boxes 33 or recovery boxes 32.

[0050] In this embodiment, Figure 1 and Figure 2 As shown, two baffles 11 are provided on the workbench 1 and located in the working position. The two baffles are provided on opposite sides of the chute. When the nozzle box 33 moves to the working position, the baffles 11 can press the nozzle box 33 to prevent the nozzle box 33 from separating from the moving part 3 when the robot arm 42 removes the nozzles. In other embodiments, four baffles 11 are provided on the workbench 1 and located in the working position, and two blocks 11 are provided on opposite sides of the chute to block the nozzle box 33.

[0051] As a preferred solution, the baffle 11 has an inverted "L" shape. The baffle 11 includes a first connecting plate and a stopper. One end of the first connecting plate is connected to the workbench 1, and the other end is connected to one end of the stopper. The other end of the stopper extends above the chute. The first connecting plate can support the stopper to a certain height to prevent the moving part 3 from colliding with the stopper when the nozzle box 33 and the recovery box 32 are moved. When the nozzle box 33 is in the working position, the stopper is located above the nozzle box 33. The stopper can press the nozzle box 33 to prevent the nozzle box 33 from detaching from the moving part 3 when the robot arm 42 removes the nozzle.

[0052] Specifically, a boss 313 is provided at one end of the movable platform 31, a first mounting groove 3131 for mounting a dye bottle is provided on the boss 313, and an observation position 3132 is provided on the side wall of the boss 313 corresponding to the first mounting groove 3131. Figure 6 As shown, a boss 313 is provided on the end of the movable platform 31 facing the operator, and two first mounting grooves 3131 are provided on the boss 313, one of which is used to install the hematoxylin bottle and the other is used to install the EA / OG bottle. An observation position 3132 is provided on the side wall of the boss 313 corresponding to the first mounting groove 3131. During operation, the storage capacity of the hematoxylin bottle and the EA / OG bottle can be directly seen through the observation position 3132.

[0053] In another embodiment, a boss 313 is provided at one end of the movable platform 31, and a first mounting groove 3131 for mounting a dye bottle is provided on the boss 313. A weight sensor is provided at the bottom of the first mounting groove 3131. The storage amount of the dye in the dye bottle is monitored by the weight sensor. The weight sensor is connected to an alarm. When the reagent in the dye bottle is reduced to a certain amount, the weight sensor sends a signal to the alarm, and the alarm gives an alarm prompt.

[0054] Specifically, the slide preparation and staining device includes a rotating arm 5, which includes a rotating shaft 51 and an arm body 52. ​​The rotating shaft 51 is rotatably arranged on the workbench 1, and one end of the rotating shaft 51 is connected to the arm body 52. ​​A liquid adding tube 521 is provided on a side of the arm body 52 close to the workbench 1. A reagent bottle 7 is provided on the workbench 1. The liquid adding tube 521 is connected to the reagent bottle 7 and can add the reagent in the reagent bottle 7 to the centrifuge tube 21 and the staining chamber 22. In this embodiment, Figure 5 and Figure 7 As shown, the liquid addition tube 521 includes an alcohol tube 5213 and a first buffer tube 5211 and a second buffer tube 5212. The reagent bottle 7 includes an alcohol bottle and a buffer bottle. The first buffer tube 5211 is located at the end away from the rotating shaft 51, and the second buffer tube 5212 and the alcohol tube 5213 are located in the middle of the arm body 52. ​​The first buffer tube 5211 and the second buffer tube 5212 are both connected to the buffer bottle, and the alcohol tube 5213 is connected to the alcohol bottle 7. When adding reagents, the first buffer tube 5211 is located above the centrifuge tube 21 and adds buffer to the centrifuge tube 21 to dilute the sample. The second buffer tube 5212 and the alcohol tube 5213 are located above the staining chamber 22 and sequentially add alcohol and buffer to the staining chamber 22. The rotating arm 5 automatically adds the required reagents to the centrifuge tube 21 and the staining chamber 22, ensuring more accurate reagent addition and more precise staining test results. In addition, the function of adding buffer solution injection pipeline is separated from the robot arm 42. By setting a rotating arm 5 on the workbench 1 to replace the injection pipeline to add buffer solution, the pipeline design is more reasonable, the failure rate is reduced, and the emptying action is also convenient.

[0055] As a preferred solution, since the centrifuge tube 21 is arranged on the upper end surface of the rotating disk 2 higher than the upper end surface of the staining chamber 22, by setting the length of the first buffer tube 5211 to be shorter than the lengths of the second buffer tube 5212 and the alcohol tube 5213, it can prevent the liquid adding tube 521 from interfering with the centrifuge tube 21 during the rotation of the rotating arm 5, and it can also prevent the buffer solution and alcohol from deviating from the staining chamber 22 or the centrifuge tube 21 when adding, causing waste of resources.

[0056] Specifically, a weight sensor is provided on the workbench 1, and the reagent bottle 7 is located on the weight sensor. In this embodiment, two second mounting slots are provided on the workbench 1, and weight sensors are installed at the bottom of the second mounting slots. The alcohol bottle and the buffer bottle are respectively installed in the two second mounting slots and abut the weight sensors. During the cell staining process, a large amount of buffer is required, and the buffer in the buffer bottle is easily used up. Therefore, the alcohol bottle and the buffer bottle are monitored by the weight sensor. The weight sensor is also connected to an alarm, which will sound an alarm when the alcohol bottle and the buffer bottle are reduced to a certain amount.

[0057] Specifically, an emptying slot 6 is provided on the workbench 1, and the emptying slot 6 is spaced apart from the rotating shaft 51. The arm body 52 can rotate to the top of the emptying slot 6 and can empty the residual reagent in the liquid adding tube 521 into the emptying slot 6. In this embodiment, Figure 4 and Figure 5 As shown, a drainage hole is provided inside the emptying tank 6, and the drainage hole is connected to the recovery box. When the arm body 52 adds reagents, the arm body 52 is located above the rotating disk 2, and the adding tube 521 adds alcohol and buffer solution to the centrifuge tube 21 and the staining chamber 22. When the arm body 52 is draining, the arm body 52 is located above the emptying tank 6, and the residual reagent in the adding tube 521 is emptied into the emptying tank 6. After the reagent flows to the recovery box through the drainage hole, the reagent in the recovery box is uniformly processed.

[0058] In this embodiment, 16 mounting plates 25 are provided on the rotating disk 2. The 16 mounting plates 25 are all located between the centrifuge tube 21 and the peripheral side of the rotating disk 2. A fixing portion 26 is convexly provided on the opposite sides of each mounting plate 25. The fixing portion 26 is provided with a fixing groove on the side wall. The notch of the fixing groove faces the center of the mounting plate 25. The upper end of the fixing portion 26 is provided with a mounting port 261. The mounting port 261 is communicated with the fixing groove. A snap-fit ​​groove is concavely provided on the groove wall of the fixing groove. The dyeing chamber 22 includes a main body 221 and two fixing members 222. The two fixing members 222 are opposite to each other. It is arranged on the outer wall of the main body 221, and the two fixing parts 222 are located at the lower end of the main body 221. A groove 27 extending to the outside of the mounting plate 25 is provided in the middle of the main body 221. The tail of the groove 27 is bent downward. The groove 27 guides the reagent in the dyeing chamber 22 when the turntable rotates to drain the liquid. The fixing part 222 is inserted into the fixing groove through the installation port 261, and is rotated to a set angle to be clamped in the clamping groove, thereby improving the installation efficiency of the dyeing chamber 22. When the rotating disk 2 drives the dyeing chamber 22 to rotate, the clamping groove can prevent the dyeing chamber 22 from shaking.

[0059] In this embodiment, a control console is provided on the side wall of the frame. The control console can simultaneously control the rotating disk 2, the mechanical arm 42, the moving part 3, and the rotating arm 5. The operator can directly operate the control console through the touch screen 8. The touch screen 8 allows the control or monitoring of each component, which is simple and efficient.

[0060] As a preferred solution, a sensor is provided at the bottom of the robotic arm 42. When the robotic arm 42 is in the first position and the robotic arm 42 grabs the suction nozzle, the robotic arm 42 grabs it according to the route set by the console. If the suction nozzle is not stored at the predetermined position, the sensor will transmit the situation back to the console, and will automatically skip the position and move to the through hole 3321 at the next position to grab the suction nozzle. This can reduce the meaningless movement of the robotic arm 42 and effectively improve the cell staining efficiency.

[0061] Taking the staining of cervical cells as an example, when the slide preparation and staining device is working, the robotic arm 42 will move to the first position, and the moving part 3 will drive the nozzle box 33 to move to the working position. After the robotic arm 42 grabs the nozzle from the nozzle box 33, the robotic arm 42 moves to the second position. When the robotic arm 42 is in the second position, the robotic arm 42 extends the nozzle into the centrifuge tube 21 and transfers the cervical cells in the centrifuge tube 21 to the staining chamber 22. After the transfer is completed, the robotic arm 42 moves to the first position to withdraw the used nozzle, and the moving part 3 drives the nozzle box 33 to move to the working position. The robotic arm 42 grabs a new nozzle from the nozzle box 33, and at the same time, the arm body 52 rotates to the top of the rotating disk 2 and remains stationary. The rotation of the rotating disk 2 drives the staining chamber 22 to move to the bottom of the arm body 52, and the liquid adding tube 521 on the arm body 52 adds alcohol and buffer to all staining chambers 22 in turn. After the flushing is completed, the arm body 52 returns to the initial position, and then the moving part 3 moves to drive the hematoxylin bottle and the EA / OG bottle to the working position. The robotic arm 42 sucks the hematoxylin through the suction nozzle and then moves to the second position to add the hematoxylin to the staining chamber 22. The robotic arm 42 moves to the first position to withdraw the used suction nozzle and replace it with a new suction nozzle. At the same time, the arm body 52 adds alcohol and buffer to the staining chamber 22 in sequence. Then the moving part 3 drives the EA / OG bottle to the working position again. The robotic arm 42 sucks the EA / OG liquid through the suction nozzle and then moves to the second position to add EA / OG to the staining chamber 22. After adding EA / OG, the robotic arm 42 moves to the first position to withdraw the used suction nozzle. At the same time, the arm body 52 adds alcohol to the staining chamber 22. After completing the above steps, the cells in the staining chamber 22 are left to stain. After the staining is completed, the rotating disk 2 rotates at high speed, and the reagent is discharged from the staining chamber 22 through the groove 27 to the outside of the rotating disk 2.

[0062] In this embodiment, Figures 8 to 10As shown, the nozzle box 33 includes a nozzle tray 332 and a box body 331. The nozzle tray 332 is arranged at the upper end of the box body 331. A plurality of through holes 3321 are spaced apart on the nozzle tray 332. The suction nozzles are arranged in the through holes 3321. A plurality of first reinforcing beams 3322 are spaced apart at the bottom of the nozzle tray 332 along the width direction of the nozzle box 33. At least two first support portions 3311 are provided inside the box body 331. The two first support portions 3311 are located on opposite sides of the box body 331, and part of the first reinforcing beams 3322 abut against the first support portions 3311. It can be understood that the nozzle tray 332 is installed at the upper end of the box body 331, and a plurality of through holes 3321 are arranged at intervals on the nozzle tray 332. The through holes 3321 are arranged in a rectangular array on the nozzle tray 332, and the nozzles can be installed in the through holes 3321. At the bottom of the nozzle tray 332, first reinforcing beams 3311 are arranged at intervals along the width direction of the nozzle box 33. The first reinforcing beams 3322 are provided at the bottom of the nozzle tray 332 to increase the strength of the nozzle tray 332. At least two first support portions 3322 are provided inside the box body 331. The two first support parts 3311 are symmetrically arranged on both sides of the box body 331. When the suction nozzle tray 332 is installed on the box body 331, the two ends of part of the first reinforcement beam 3322 respectively abut on the two first support parts 3311 that are relatively arranged, so that the first support parts 3311 and the side walls of the box body 331 jointly support the downward force, and the first support parts 3311 abut against the first reinforcement beam 3322, so that the force on the entire suction nozzle box 33 is more uniform and the strength is higher, and it is not easy to be damaged due to deformation when the operator uses the suction nozzle box 33.

[0063] It should be noted that the downward force includes the weight of the nozzle tray 332 and the nozzles stored on the nozzle tray 332 and the force applied by the operator when placing or grabbing the nozzles in the nozzle box 33 .

[0064] Specifically, a first cavity 33111 is provided in the first support portion 3311, and at least part of the suction nozzle is located in the first cavity 33111. In this embodiment, the first support portion 3311 includes a third support plate, a fourth support plate, and a fifth support plate. One side of the third support plate is connected to the inner side wall of the box body 331, and the other side is connected to one side of the fourth support plate. One side of the fifth support plate is connected to the inner side wall of the box body 331, and the other side is connected to the fourth support plate. The third support plate and the fifth support plate are spaced apart. The third support plate, the fourth support plate, and the fifth support plate are connected to the inner side wall of the box body 331 to form the above-mentioned first cavity 33111. When the suction nozzle is installed in the through hole 3321, part of the suction nozzle can extend into the first cavity 33111, so that the first support portion 3311 is added to the box body 331 to support the suction nozzle tray 332, but does not affect the number of suction nozzles that can be stored in the suction nozzle tray 332.

[0065] Specifically, there are four first support parts 3311, and every two first support parts 3311 form a support group. The two support groups are spaced apart along the length direction of the box body 331, and the two support groups are symmetrically arranged along the center of the length direction of the box body 331. The four first support parts 3311 are distributed in a rectangular shape to form a support surface for supporting the suction nozzle tray 332, so that the force area of ​​the box body 331 is larger and more balanced.

[0066] Specifically, a second support portion 3312 is further provided in the box body 331. The second support portion 3312 is spaced apart from the first support portion 3311. A plurality of second reinforcing beams 3323 are spaced apart at the bottom of the nozzle tray 332 along the long side direction of the nozzle box 33. The second reinforcing beams 3323 abut against the second support portion 3312. In this embodiment, the second support portion 3312 is provided between two groups of first support portions 3311. The top surface of the second support portion 3312 is flush with the top surface of the first support portion 3311. The first support portion 3311 and the second support portion 3312 jointly support the nozzle tray 332, increasing the support area of ​​the box body 331 for the nozzle tray 332. The plurality of second reinforcing beams 3323 are provided along the second direction at the bottom of the nozzle tray 332 to increase the strength of the nozzle tray 332. The second reinforcing beams 3323 and part of the first reinforcing beams 3322 abut against the second support portion 3312, thereby improving the overall strength of the nozzle box 33.

[0067] As a preferred solution, the second support portion 3312 is supported in the middle of the nozzle tray 332, and an avoidance position is provided on the second support portion 3312 corresponding to the through hole 3321 on the nozzle tray 332. Since the middle of the nozzle tray 332 is subjected to the least support force, the downward force is most likely to cause deformation of the middle of the nozzle tray 332. Therefore, the second support portion 3312 is provided in the middle of the box body 331 and supports the middle of the nozzle tray 332 to effectively offset part of the downward force, avoid deformation of the nozzle tray 332, and thus increase the service life of the nozzle tray 332. The avoidance hole provided on the second support portion 3312 enables the second support portion 3312 to provide support to the nozzle tray 332 without affecting the number of nozzles that can be stored on the nozzle tray 332.

[0068] Specifically, the second support portion 3312 includes a second connecting plate 33123, a first support plate 33121 and a second support plate 33122. The first support plate 33121 and the second support plate 33122 are spaced apart. The opposite ends of the first support plate 33121 and the second support plate 33122 are respectively connected to the two opposite inner walls of the box body 331. The second connecting plate 33123 connects the first support plate 33121 and the second support plate 33122. The second connecting plate 33123 is spaced apart from the inner wall of the box body 331, and an avoidance position is formed between the second connecting plate 33123, the first support plate 33121 and the second support plate 33122. In this embodiment, the first support plate 33121 and the second support plate 33122 have the same shape and structure. The first support plate 33121 and the second support plate 33122 are spaced apart and arranged parallel to each other within the box body 331. The opposing side surfaces of the first support plate 33121 and the second support plate 33122 are connected to the opposing inner sidewalls of the box body 331, thereby increasing the strength of the box body 331. Two second connecting plates 33123 are provided, each connecting the first support plate 33121 and the second support plate 33122. Both second connecting plates 33123 are spaced apart from the sidewalls of the box body 331, thereby increasing the stability of the second support portion 3312 and further strengthening the box body 331.

[0069] In this embodiment, a mounting portion 3313 is provided in an annular manner on the inner side wall of the open end of the box body 331. The mounting portion 3313 is spaced apart from the end surface of the open end of the box body 331. The nozzle tray 332 is provided in the box body 331, and the nozzle tray 332 abuts against the mounting portion 3313. Specifically, the box body 331 includes a bottom plate, a first surrounding wall, a third connecting plate, and a second surrounding wall. The first surrounding wall is provided on the bottom plate. An end of the first surrounding wall away from the bottom plate is connected to an end of the third connecting plate. The other end of the third connecting plate extends away from the center of the box body 331 and is connected to the second surrounding wall. The first surrounding wall is connected to the bottom plate to form a first empty slot. The first supporting portion 3311 and the second supporting portion 3312 are provided in the first empty slot. The second surrounding wall is connected to the third connecting plate to form a second empty slot. The mounting portion 3313 is located on the third connecting plate. The nozzle tray 332 can be directly installed in the second empty slot and abut against the mounting portion 3313, thereby improving the installation efficiency of the nozzle tray 332.

[0070] To facilitate installation and removal of the nozzle tray 332 from the box body 331, latches 3324 are provided on opposite sides of the nozzle tray 332. Protrusions 3314 are provided on the inner sidewall of the box body 331, corresponding to the latches 3324. Two protrusions 3314 are provided on the same sidewall. In other embodiments, only one or more protrusions 3314 may be provided on the same sidewall. The protrusions 3314 are provided adjacent to the end surface of the open end of the box body 331, and the latches 3324 engage with the protrusions 3314.

[0071] In this embodiment, a plurality of limiting parts are provided on the mounting portion 3313, and the limiting parts include a first limiting member 33131 and a second limiting member 33132. The first limiting member 33131 and the second limiting member 33132 are spaced apart along the length direction of the mounting portion 3313. Along the width direction of the mounting portion 3313, the first limiting member 33131 is located at one end of the mounting portion 3313 away from the center of the box body 331. The first limiting member 33131 is connected to the inner side wall of the open end of the box body 331, and the second limiting member 331 is connected to the inner side wall of the open end of the box body 331. 32 is located at one end of the mounting portion 3313 close to the center of the box body 331, and a second cavity 3325 is provided along one side of the nozzle tray 332 close to the mounting portion 3313. The second limiting member 33132 abuts against the cavity wall of the second cavity 3325, and the first limiting member 33131 abuts against the outer wall of the nozzle tray 332. The first limiting member 33131 and the second limiting member 33132 respectively limit the inner and outer walls of the nozzle tray 332 to prevent the nozzle tray 332 from shaking laterally on the mounting portion 3313.

[0072] In order to prevent the operator from slipping when grabbing the nozzle box 33 , an anti-slip groove 3315 is recessed on the outer wall of the box body 331 , and a plurality of anti-slip grooves 3315 are arranged at intervals.

[0073] In the description of this article, it should be understood that the terms "upper", "lower", etc., and the orientation or position relationship are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be understood as a limitation on the present invention.

[0074] In the description of this specification, the description of reference terms such as "an embodiment" means that the specific features, structures, materials, or characteristics of the embodiment are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment.

[0075] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

[0076] The technical principles of the present invention have been described above with reference to specific embodiments. These descriptions are intended solely to illustrate the principles of the present invention and are not to be construed in any way as limiting the scope of protection of the present invention. Based on the explanations herein, those skilled in the art will readily conceive of other specific embodiments of the present invention without inventive effort, and such embodiments will fall within the scope of protection of the present invention.

Claims

1. A film making and dyeing device, characterized in that: include: Workbench; A rotating disk, the rotating disk is arranged on the workbench, a plurality of centrifuge tubes are arranged in an annular manner on the rotating disk, and dyeing chambers are arranged on the rotating disk corresponding to the centrifuge tubes at intervals; a moving portion, the moving portion being spaced apart and arranged on one side of the rotating disk, the moving portion being provided with a recovery box and a nozzle box, the moving portion being capable of driving the recovery box and the nozzle box to reciprocate along a first direction so that the recovery box and the nozzle box are alternately located at a working position; a nozzle driving member, the nozzle driving member comprising a crossbeam disposed above the workbench, a robotic arm capable of reciprocating along a second direction being slidably disposed on the crossbeam, the robotic arm having a first position and a second position, wherein when the robotic arm is located at the first position, the robotic arm moves to the working position to grab a nozzle or eject a used nozzle, and when the robotic arm is located at the second position, the robotic arm transfers cells in the centrifuge tube to the staining chamber; The rotating disk includes a first rotating disk and a second rotating disk, the first rotating disk and the second rotating disk are arranged on the workbench at intervals, and the robotic arm includes a first robotic arm arranged corresponding to the first rotating disk and a second robotic arm arranged corresponding to the second rotating disk; The workbench is provided with a slide groove, the slide groove is located between the first turntable and the second turntable, and the moving part is arranged in the slide groove; The moving portion includes a moving platform, wherein a first mounting position and a second mounting position are provided on the moving platform along a moving direction of the moving platform, the recovery box is provided in the first mounting position, and the nozzle box is provided in the second mounting position, and one of the first mounting position and the second mounting position is selectively located in the working position; A boss is provided at one end of the movable platform, and a first mounting groove for mounting a dye bottle is provided on the boss.

2. The film making and dyeing device according to claim 1, characterized in that: At least two baffles are provided on the workbench and at the working position. The two baffles are respectively provided on opposite sides of the slide groove. When the nozzle box moves to the working position, the baffles press the nozzle box to prevent the nozzle box from separating from the moving part when the robot arm takes out the nozzle.

3. The film making and dyeing device according to claim 1, characterized in that: It includes a rotating arm, which includes a rotating shaft and an arm body. The rotating shaft is rotatably set on the workbench, one end of the rotating shaft is connected to the arm body, and a liquid adding tube is provided on a side of the arm body close to the workbench. A reagent bottle is provided on the workbench, and the liquid adding tube is connected to the reagent bottle. The liquid adding tube can add the reagent in the reagent bottle to the centrifuge tube and the staining chamber.

4. The film making and dyeing device according to claim 3, characterized in that: An observation position is provided on the side wall of the boss corresponding to the first mounting groove; or, A weight sensor is provided at the bottom of the first installation groove.

5. The film making and dyeing device according to claim 4, characterized in that: A weight sensor is arranged on the workbench, and the reagent bottle is arranged on the weight sensor.

6. The film making and dyeing device according to claim 4, characterized in that: An emptying trough is provided on the workbench and is spaced apart from the rotating shaft. The arm body can rotate to the top of the emptying trough and can empty the residual reagent in the liquid adding tube into the emptying trough.

7. The film making and dyeing device according to claim 1, characterized in that: The rotating disk is provided with a mounting plate, the mounting plate is located between the centrifuge tube and the peripheral side of the rotating disk, the mounting plate is provided with a fixing portion protruding on opposite sides, the fixing portion is provided with a fixing groove on the side wall, the notch of the fixing groove faces the center of the mounting plate, the upper end of the fixing portion is provided with a mounting port, the mounting port is communicated with the fixing groove, and the groove wall of the fixing groove is concavely provided with a clamping groove; The dyeing chamber includes a main body and two fixing parts, which are arranged on the outer side wall of the main body relative to each other. The fixing parts are inserted into the fixing groove through the installation opening and rotated at a set angle to be clamped in the clamping groove.

Images