A fully automatic chromosome preparation device

Abstract

A fully automatic chromosome preparation device includes: a culture mechanism, including an incubator for placing culture dishes; a storage and retrieval mechanism, including a clamping platform and a first transport component, the first transport component is used to drive the clamping platform to store and retrieve culture dishes in the incubator; a centrifugal mechanism, including a centrifugal rack for placing culture dishes and a centrifugal power source for driving the centrifugal rack to rotate horizontally; a processing mechanism, including a mounting seat and a second transport component, the second transport component is used to drive the mounting seat to move between the clamping platform and the centrifugal rack, the mounting seat is provided with a grabbing component for grabbing the culture dish, a capping component for opening the cover of the culture dish, and a sample adding component for adding reagents to the culture dish. The above scheme realizes the fully automatic operation of a series of processes such as culturing, transporting, sample adding, centrifuging, etc. of the culture dish, which improves the preparation efficiency while reducing the possibility of contamination and effectively avoiding human intervention.

Description

Technical Field

[0001] The present invention relates to the technical field of biomass preparation equipment, and in particular to a fully automatic chromosome preparation equipment. Background Art

[0002] Cytogenetics technology diagnoses possible chromosomal defects by analyzing chromosomes under a microscope. In clinical practice, chromosome preparation requires culturing cells first, followed by multiple steps such as blocking, harvesting, hypotonicity, fixation, drying, aging, enzyme digestion, and banding before chromosomes can be analyzed under a microscope to determine whether there are chromosomal abnormalities. This traditional method has been used to this day.

[0003] Harvesting cultured cells is an important daily work link in the cell genetics laboratory. The basic process of this link is as follows: transfer the culture dish after culturing in the incubator for a period of time to the centrifuge, and after the centrifugation operation, aspirate the supernatant of the sample in the culture dish and discard it; then add hypotonic solution to the culture dish for a period of time and then add fixative for pre-fixation; centrifuge again and aspirate the supernatant, add new fixative to mix and fix; centrifuge again and aspirate the supernatant, add new fixative to mix and fix, repeat this 3 times; finally, harvest the sample cells. As can be seen from the above, there are many repeated operations such as aspiration, addition of liquid, mixing, centrifugation, etc. in the process of harvesting the target sample. If each step is completed one by one by hand for a single specimen, it is obviously cumbersome, time-consuming and laborious. Summary of the invention

[0004] The problem solved by the invention is to provide a fully automatic chromosome preparation device which can automatically realize a series of operations such as culturing, transporting, adding samples and centrifuging on a culture dish.

[0005] In order to solve the above problems, the present invention provides a fully automatic chromosome preparation device, comprising:

[0006] A culture mechanism, including an incubator for placing culture dishes;

[0007] The storage and retrieval mechanism comprises a clamping platform and a first transport component, wherein the first transport component is used to drive the clamping platform to store and retrieve the culture dish in the incubator;

[0008] A centrifugal mechanism, comprising a centrifugal frame for placing a culture dish and a centrifugal power source for driving the centrifugal frame to rotate horizontally;

[0009] The processing mechanism includes a mounting seat and a second transport component, wherein the second transport component is used to drive the mounting seat to move between the clamping table and the centrifuge frame, and the mounting seat is provided with a grabbing component for grabbing the culture dish, a screwing component for opening the cover of the culture dish, and a sample adding component for adding reagents to the culture dish.

[0010] Compared with the prior art, the beneficial effects of the above scheme are as follows: through the setting of the storage and access structure, the clamping table can store and access the culture dishes in the incubator, or the clamping table can also directly place new culture dishes added from the outside; through the setting of the mounting seat and the second transfer component, the grabbing component can transfer the culture dishes between the clamping table and the centrifuge rack, and the capping component and the sample loading component can also directly perform capping or sample loading operations on the culture dishes on the clamping table, thereby realizing the full automatic operation of a series of processes such as culturing, transporting, sample loading, and centrifugation of the culture dishes, while improving the preparation efficiency and reducing the possibility of contamination, and effectively avoiding the operational risks caused by manual intervention.

[0011] Preferably, a transfer mechanism is further included, which includes a clamp and an adjusting motor, wherein the clamp is located within the moving range of the mounting seat to clamp the culture dish, and the adjusting motor is used to drive the clamp to rotate to dump the culture dish, so that the grabbing assembly on the mounting seat can first transfer the culture dish on the clamp to the clamp for temporary storage, and then the capping assembly or the sample loading assembly on the mounting seat can more conveniently perform corresponding operations on the culture dish on the clamp, and the clamp can also be driven by the adjusting motor to rotate to dump the sample in the culture dish.

[0012] Preferably, the second transport component includes a translation drive for pushing the mounting seat to move horizontally and a lifting drive for pushing the mounting seat to move vertically, so that the grasping component on the mounting seat can better grasp the culture dish on the clamping table or the clamping claw, and facilitate the capping and sample loading operations on the culture dish by the capping component and the sample loading component.

[0013] Preferably, it also includes a barcode scanner for identifying the culture dish, the barcode scanner is arranged toward the grasping component, the grasping component includes a first motor and a suction cup for holding the culture dish, the first motor is used to drive the suction cup to rotate, so that when the culture dish needs to be scanned and identified, the first motor can drive the suction cup to rotate, so that the barcode scanner can identify the identification information on the culture dish held by the suction cup, thereby achieving accurate recording.

[0014] Preferably, the sample loading component includes a sample loading needle, a sample loading rack and a second motor, the sample loading rack is provided with a plurality of slots for detachable connection of the sample loading needle, the second motor is used to drive the sample loading rack to rotate, so that by installing a plurality of sample loading needles loaded with various reagents into the slots and driving the sample loading rack to rotate by the second motor, various reagents can be conveniently and quickly added to the culture dish for reaction, and the preparation efficiency is high.

[0015] Preferably, the screw cap assembly includes a buckle cover and a third motor, and a screw groove matching the cover body of the culture dish is provided on the side of the buckle cover facing the culture dish, and the third motor is used to drive the buckle cover to rotate, so that when the buckle cover moves toward the culture dish driven by the mounting seat, the cover body of the culture dish can be stuck in the inside of the screw groove and realize the screwing out or screwing in function under the drive of the third motor.

[0016] Preferably, the incubator is provided with a turntable rack for placing culture dishes, the turntable rack is rotatably connected to the incubator, the turntable rack is provided with a plurality of through slots arranged along the circumferential direction, the through slots all extend to the outer edge of the turntable rack, the width of the through slots is smaller than the culture dishes so that the culture dishes can be placed on top of the through slots, the side walls of the incubator are provided with operating windows for the clamp to pass through, the first transport component comprises a transverse driving member for pushing the clamp into the incubator and a vertical driving member for pushing the clamp to rise and fall, so that the clamp can conveniently pass through the through slots from below and perform storage and retrieval operations on the culture dishes on the turntable rack, the structure is simple and the operation is reliable, the equipment cost is effectively reduced, and it has good practicality.

[0017] Preferably, the centrifuge frame includes a main frame connected to the centrifugal power source and a plurality of sub-frames for fixing culture dishes, the sub-frames are arranged along the circumference of the main frame, the sub-frames are rotatably connected to the main frame and the rotation axes are arranged radially perpendicular to the main frame, so that as the rotation speed of the centrifuge frame changes, the culture dishes can rotate within a certain range to achieve a better centrifugal effect.

[0018] As preferably, the centrifugal mechanism also includes an eccentric sleeve, an oscillation power source and a sliding assembly, the sub-frame includes an outer ring frame and an inner ring frame, the outer ring frame is rotatably connected to the centrifugal frame and the rotation axis is perpendicular to the radial direction of the centrifugal frame, the inner ring frame is rotatably connected to the inner side of the outer ring frame and the rotation axis is parallel to the radial direction of the centrifugal frame, the inner ring frame is provided with a shelf for fixing the culture dish and a transmission rod arranged vertically, the sliding assembly is used to push the eccentric sleeve to move vertically so that the lower end of the transmission rod is inserted into the eccentric sleeve, the oscillation power source is used to drive the eccentric sleeve to rotate, so that when the sample is temporarily centrifuged, the eccentric sleeve can move toward the transmission rod under the drive of the sliding assembly until the transmission rod is inserted into the eccentric sleeve, and then the oscillation power source drives the eccentric sleeve to rotate, so that the sample on the sub-frame is shaken and mixed. After the shaking is completed, the sliding assembly drives the eccentric sleeve to reset, and the sample can continue to be centrifuged or other subsequent operations as needed. Compared with the prior art, the above scheme can simultaneously realize centrifugation and shaking operations without transferring the sample, with high preparation efficiency and no pollution.

[0019] Preferably, it also includes a waste liquid treatment mechanism, which includes an inlet pipe, an exhaust pipe and a box body provided with a accommodating chamber, the inlet pipe and the exhaust pipe are both connected to the accommodating chamber, the upper part of the inlet pipe is arranged toward the clamp, the middle part of the inlet pipe is provided with a buffer section that can accommodate waste liquid, a first valve body is provided above the buffer section, a second valve body is provided below the buffer section, and the exhaust pipe is provided with a third valve body; the first valve body is used to open when waste liquid enters the inlet pipe, and to close after the waste liquid enters the buffer section; the second valve body and the third valve body are both used to open after the waste liquid enters the buffer section and the first valve body is closed, and to close after the waste liquid enters the accommodating chamber. After adopting the above structure, in the idle state, the first valve body and the second valve body on the liquid inlet pipe and the third valve body on the exhaust pipe are all in the closed state, ensuring the sealing of the waste liquid in the receiving chamber; when new waste liquid needs to be dumped, the first valve body is first opened, and the new waste liquid enters the buffer section through the first valve body, and then the first valve body is closed, and the second valve body and the third valve body are automatically opened. At this time, the new waste liquid in the buffer section can flow through the second valve body and enter the receiving chamber for temporary storage, and the third valve body is used to realize the volume compensation adjustment of the exhaust pipe to the waste liquid in the receiving chamber, and finally the second valve body and the third valve body return to the closed state. Compared with the prior art, the above scheme avoids the problem of waste liquid volatilization in the receiving chamber when dumping waste liquid, effectively reduces the degree of pollution, and can realize fully automatic operation, with good practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is an axonometric diagram of a fully automatic chromosome preparation device;

[0021] Figure 2 It is a schematic diagram of the main view of a fully automatic chromosome preparation device;

[0022] Figure 3 A schematic top view of a fully automatic chromosome preparation device;

[0023] Figure 4 It is an axonometric diagram of a mounting base of a fully automatic chromosome preparation device;

[0024] Figure 5 It is an axonometric schematic diagram of a centrifugal mechanism of a fully automatic chromosome preparation device;

[0025] Figure 6 A schematic diagram of a centrifugal mechanism of a fully automatic chromosome preparation device;

[0026] Figure 7 It is an axonometric schematic diagram of a waste liquid treatment mechanism of a fully automatic chromosome preparation device;

[0027] Figure 8A schematic top view of an incubator of a centrifugal mechanism of a fully automatic chromosome preparation device after the top shell is hidden.

[0028] Description of reference numerals:

[0029] 1-incubator; 101-operation window; 102-annular groove; 103-door panel; 110-turntable rack; 111-through groove;

[0030] 2-clamping platform; 201-lateral driving member; 202-vertical driving member;

[0031] 3-gripper; 301-adjusting motor;

[0032] 4-mounting seat; 401-translation drive member; 402-lifting drive member; 410-suction cup; 411-first motor; 420-sample needle; 421-sample rack; 421a-slot; 422-second motor; 430-buckle cover; 431-rotation groove; 432-third motor; 440-barcode scanner;

[0033] 5-centrifugal frame; 501-centrifugal power source; 510-main frame; 511-guide rail; 512-spring; 513-rotating shaft; 520-sub-frame; 521-outer ring frame; 522-inner ring frame; 523-transmission rod; 530-eccentric sleeve; 531-oscillation power source; 540-sliding assembly;

[0034] 6-box; 601-liquid inlet pipe; 601a-buffer section; 602-exhaust pipe; 603-first valve body; 604-second valve body; 605-third valve body. DETAILED DESCRIPTION

[0035] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail in conjunction with the accompanying drawings. The technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. It should also be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, inside, outside) are only used to explain the relative position relationship, movement, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0036] See also Figure 1-Figure 8 , an embodiment of the present invention provides a fully automatic chromosome preparation device, comprising:

[0037] A culture mechanism, comprising a culture box 1 for placing culture dishes;

[0038] The storage and retrieval mechanism comprises a clamping platform 2 and a first transport component, wherein the first transport component is used to drive the clamping platform 2 to store and retrieve the culture dishes in the incubator 1;

[0039] The centrifugal mechanism comprises a centrifugal frame 5 for placing the culture dish and a centrifugal power source 501 for driving the centrifugal frame 5 to rotate horizontally;

[0040] The processing mechanism includes a mounting seat 4 and a second transport component, the second transport component is used to drive the mounting seat 4 to move between the clamping table 2 and the centrifuge frame 5, and the mounting seat 4 is provided with a grabbing component for grabbing the culture dish, a screwing component for opening the cover of the culture dish, and a sample adding component for adding reagents to the culture dish.

[0041] The above scheme enables the clamping table 2 to store and retrieve the culture dishes in the incubator 1 through the setting of the storage and access structure. Of course, the clamping table 2 can also directly place new culture dishes added from the outside; through the setting of the mounting seat 4 and the second transfer component, the grabbing component can transfer the culture dishes between the clamping table 2 and the centrifuge rack 5, and the capping component and the sample loading component can also directly perform capping or sample loading operations on the culture dishes on the clamping table 2, thereby realizing fully automatic operation of a series of processes such as culturing, transporting, sample loading, and centrifugation of the culture dishes, while improving the preparation efficiency and reducing the possibility of contamination, and effectively avoiding operational risks caused by manual intervention.

[0042] In the present embodiment, a transfer mechanism is also included, and the transfer mechanism includes a clamping jaw 3 and an adjusting motor 301 arranged in the transverse direction. The clamping jaw 3 is located within the moving range of the mounting seat 4 to clamp the culture dish. The adjusting motor 301 is used to drive the clamping jaw 3 to rotate to dump the culture dish, so that the grabbing assembly on the mounting seat 4 can first transfer the culture dish on the clamping platform 2 to the clamping jaw 3 for temporary storage, and then the capping assembly or the sample loading assembly on the mounting seat 4 can more conveniently perform corresponding operations on the culture dish on the clamping jaw 3, or the adjusting motor 301 directly drives the clamping jaw 3 to rotate to dump the culture dish. It should be understood that the clamping jaw 3 is located within the moving range of the mounting seat 4, which means that the grabbing assembly, the capping assembly and the sample loading assembly on the mounting seat 4 can all contact the culture dish on the clamping jaw 3 to achieve the corresponding function.

[0043] The second transfer component includes a translation drive 401 for pushing the mounting seat 4 to move horizontally and a lifting drive 402 for pushing the mounting seat 4 to move vertically. In this embodiment, the translation drive 401 is an electric slide rail arranged horizontally, and the lifting drive 402 is an electric slide rail arranged vertically, so that the grasping component on the mounting seat 4 can better grasp the culture dish on the clamping table 2 or the clamping claw 3, and it is convenient for the capping component and the sample loading component to perform capping and sample loading operations on the culture dish.

[0044] The gripping assembly may refer to any mechanism capable of gripping the culture dish, such as a clamping manipulator. In this embodiment, the gripping assembly is a suction cup 410, the upper end of which is connected to the negative pressure pipeline, and the lower end of which can hold the culture dish.

[0045] Furthermore, it also includes a barcode scanner 440 for identifying the culture dish. The barcode scanner 440 can be connected to the mounting base 4 or independently arranged on the side of the mounting base 4. The barcode scanner 440 is arranged toward the grasping assembly. The grasping assembly also includes a first motor 411 for starting the rotation of the suction cup 410, so that when it is necessary to scan and identify the culture dish, the first motor 411 can drive the suction cup 410 to rotate, so that the barcode scanner 440 can identify the identification information on the culture dish held by the suction cup 410, and realize accurate recording.

[0046] In this embodiment, the sample loading component includes a sample loading needle 420, a sample loading rack 421 and a second motor 422. The sample loading rack 421 is provided with a plurality of slots 421a for detachably connecting the sample loading needle 420. The second motor 422 is used to drive the sample loading rack 421 to rotate. Therefore, by installing a plurality of sample loading needles 420 containing various reagents into the slots 421a and driving the sample loading rack 421 to rotate by the second motor 422, various reagents can be conveniently and quickly added to the culture dish for reaction, and the preparation efficiency is high.

[0047] In this embodiment, the cap screwing assembly includes a buckle cover 430 and a third motor 432. The lower end surface of the buckle cover 430 is provided with a rotation groove 431 matching the cover body of the culture dish. The third motor 432 is used to drive the buckle cover 430 to rotate, so that when the buckle cover 430 moves toward the culture dish driven by the mounting seat 4, the cover body of the culture dish can be inserted into the inside of the rotation groove 431 and realize the function of screwing out or screwing in under the drive of the third motor 432. In this embodiment, the rotation groove 431 is circular and the groove diameter gradually decreases in the upward direction, so that when the cap screwing assembly moves toward the culture dish driven by the mounting seat 4, the cover body of the culture dish can be gradually inserted into the rotation groove 431 and temporarily fixed. When the cover body is completely screwed down, the mounting seat 4 slides, and after the sample loading assembly performs the sample loading operation, the mounting seat 4 drives the cap screwing assembly to move toward the culture dish again and screws the cover body back to the culture dish. It should be understood that the shape of the rotary groove 431 only needs to match the cover body, and the present design does not limit this. In addition, the groove diameter of the rotary groove 431 can also be constant. In this case, only a retaining ring needs to be provided in the rotary groove 431 to achieve temporary fixation of the cover body.

[0048] A turntable frame 110 for placing culture dishes is provided in the incubator 1, and the turntable frame 110 is rotatably connected to the incubator 1. A plurality of through slots 111 arranged along the circumferential direction are provided on the turntable frame 110, and the through slots 111 all penetrate to the outer edge of the turntable frame 110. The width of the through slots 111 is smaller than the culture dishes so that the culture dishes can be placed on the through slots 111. An operating window 101 for the clamping platform 2 to pass through is provided on the side wall of the incubator 1. The first transport component includes a horizontal driving member 201 for pushing the clamping platform 2 into the incubator 1 and a vertical driving member 202 for pushing the clamping platform 2 to rise and fall. In this embodiment, the horizontal driving member 201 is a ball screw motor pair arranged along the horizontal direction, and the vertical driving member 202 is a ball screw motor pair arranged along the vertical direction, so that the clamping platform 2 can easily pass through the through slots 111 from below and perform storage and retrieval operations on the culture dishes on the turntable frame 110. The structure is simple and the operation is reliable, which effectively reduces the equipment cost and has good practicality.

[0049] Furthermore, the side wall of the incubator 1 is slidably connected with a door panel 103 for closing the operation window 101 and an annular groove 102 arranged around the operation window 101, and an inflatable air cushion is arranged in the annular groove 102, and the air cushion is used to abut against the door panel 103 to achieve better sealing.

[0050] The centrifuge frame 5 includes a main frame body 510 connected to a centrifugal power source 501 and a plurality of sub-frame bodies 520. In the present embodiment, there are four sub-frame bodies 520 and the sub-frame bodies 520 are evenly arranged along the circumference of the main frame body 510. The sub-frame bodies 520 are rotatably connected to the main frame body 510 and the rotating shaft 513 is arranged along the radial direction perpendicular to the main frame body 510. The sub-frame body 502 is provided with a shelf portion for inserting a culture dish, so that as the rotation speed of the centrifuge frame 5 is different, the culture dish can rotate within a certain range to achieve a better centrifugal effect.

[0051] Further, the centrifugal mechanism also includes an eccentric sleeve 530, an oscillation power source 531 and a sliding assembly 540, and the sub-frame 520 includes an outer ring frame 521 and an inner ring frame 522, the outer ring frame 521 is rotatably connected to the centrifugal frame 5 and the rotating shaft 513 is perpendicular to the radial direction of the centrifugal frame 5, the inner ring frame 522 is rotatably connected to the inner side of the outer ring frame 521 and the rotating shaft 513 is parallel to the radial direction of the centrifugal frame 5, and the inner ring frame 522 is provided with a shelf for plugging in a culture dish and a transmission rod 523 arranged vertically. The sliding assembly 540 is used to push the eccentric sleeve 530 to move vertically so that the lower end of the transmission rod 523 is inserted into the eccentric sleeve 530, and the oscillation power source 531 is used to drive the eccentric sleeve 530 to rotate, so that when the sample is temporarily centrifuged, the eccentric sleeve 530 can move to the transmission rod 523 under the drive of the sliding assembly 540 until the transmission rod 523 is inserted into the eccentric sleeve 530, and then the oscillation power source 531 drives the eccentric sleeve 530 to rotate, so that the sample on the sub-frame 520 is shaken and mixed. After the shaking is completed, the sliding assembly 540 drives the eccentric sleeve 530 to reset, and the sample can continue to be centrifuged or other subsequent operations as needed. Compared with the prior art, the above scheme can realize centrifugation and shaking operations at the same time without transferring the sample, and the preparation efficiency is high, and it will not cause pollution. In addition, a raised round table can be set at the lower end of the transmission rod 523, so as to better match the eccentric sleeve 530.

[0052] As a further optimization of the centrifugal mechanism, the outer periphery of the main frame 510 is provided with four circumferentially arranged shelf grooves for accommodating the outer ring frame 521, and the groove wall of the shelf groove is provided with a guide rail 511 arranged along the radial direction of the main frame 510, and the guide rail 511 is slidably connected with a rotating shaft 513 arranged along the radial direction perpendicular to the main frame 510, and the main frame 510 is provided with a spring 512 arranged along the radial direction, and the spring 512 is used to push the rotating shaft 513 to slide along the radial direction of the main frame 510, and the outer ring frame 521 is rotatably connected to the rotating shaft 513 of the shelf groove. The setting of the spring 512 further reduces the impact of the vibration of the sub-frame 520 on the main frame 510, and the spring 512 can play a buffering role during the centrifugal operation, so that the sub-frame 520 can be more stably rotated and centrifuged under the drive of the main frame 510.

[0053] As an extension of the above embodiment, a waste liquid treatment mechanism is also included, which includes a liquid inlet pipe 601, an exhaust pipe 602 and a box body 6 provided with a receiving chamber, and the liquid inlet pipe 601 and the exhaust pipe 602 are both connected to the receiving chamber. The upper part of the liquid inlet pipe 601 is arranged toward the clamping jaw 3, and a funnel is arranged at the top of the liquid inlet pipe 601, and a flushing assembly is arranged on the funnel to realize the cleaning of the funnel; a buffer section 601a for accommodating waste liquid is arranged in the middle part of the liquid inlet pipe 601, a first valve body 603 is arranged above the buffer section 601a, a second valve body 604 is arranged below the buffer section 601a, and a third valve body 605 is arranged on the exhaust pipe 602; the first valve body 603 is used to open when the waste liquid enters the liquid inlet pipe 601, and close after the waste liquid enters the buffer section 601a; the second valve body 604 and the third valve body 605 are both used to open after the waste liquid enters the buffer section 601a and the first valve body 603 is closed, and close after the waste liquid enters the receiving chamber. The regulating motor 301 is used to drive the clamping jaw 3 to rotate so that the sample in the culture dish is poured into the liquid inlet pipe 601. Further, the buffer section 601a is a horizontal "S" shape, so as to achieve the accommodation and buffering of new waste liquid on the one hand, and the accommodation and buffering of volatile gas of waste liquid in the accommodation chamber on the other hand. After adopting the above structure, in the idle state, the first valve body 603 and the second valve body 604 on the liquid inlet pipe 601 and the third valve body 605 on the exhaust pipe 602 are all in the closed state, ensuring the sealing of the waste liquid in the receiving chamber; when it is necessary to dump new waste liquid, first the first valve body 603 is opened, and the new waste liquid enters the buffer section 601a through the first valve body 603, then the first valve body 603 is closed, and the second valve body 604 and the third valve body 605 are automatically opened, at this time the new waste liquid in the buffer section 601a can flow through the second valve body 604 and enter the receiving chamber for temporary storage, and the third valve body 605 is used to realize the exhaust pipe 602 to compensate for the volume of the waste liquid in the receiving chamber, and finally the second valve body 604 and the third valve body 605 are restored to the closed state. Compared with the prior art, the above scheme avoids the problem of waste liquid volatilization in the receiving chamber when dumping waste liquid, effectively reduces the degree of pollution, and can realize full automatic operation, with good practicality.

[0054] Although the disclosure is disclosed as above, the protection scope of the disclosure is not limited thereto. For those skilled in the art, various changes and modifications can be made without departing from the spirit and scope of the disclosure, and these changes and modifications will fall within the protection scope of the present invention.

Claims

1. A fully automatic chromosome preparation device, characterized in that: include: A culture mechanism, comprising a culture box (1) for placing a culture dish; The storage and retrieval mechanism comprises a clamping platform (2) and a first transport component, wherein the first transport component is used to drive the clamping platform (2) to store and retrieve the culture dishes in the incubator (1); A centrifugal mechanism, comprising a centrifugal frame (5) for placing a culture dish and a centrifugal power source (501) for driving the centrifugal frame (5) to rotate horizontally; The processing mechanism comprises a mounting seat (4) and a second transport component, wherein the second transport component is used to drive the mounting seat (4) to move between the clamping platform (2) and the centrifugal frame (5), and the mounting seat (4) is provided with a grasping component for grasping a culture dish, a capping component for opening the cover of the culture dish, and a sample adding component for adding reagents to the culture dish; It also includes a transfer mechanism, the transfer mechanism including a clamping jaw (3) and an adjustment motor (301), the clamping jaw (3) is located within the moving range of the mounting seat (4) to achieve clamping of the culture dish, and the adjustment motor (301) is used to drive the clamping jaw (3) to rotate to tip the culture dish; The invention also comprises a waste liquid treatment mechanism, the waste liquid treatment mechanism comprising a liquid inlet pipe (601), an exhaust pipe (602) and a box body (6) provided with a receiving chamber, the liquid inlet pipe (601) and the exhaust pipe (602) are both connected to the receiving chamber, the upper part of the liquid inlet pipe (601) is arranged toward the clamping jaw (3), the middle part of the liquid inlet pipe (601) is provided with a buffer section (601a) capable of accommodating waste liquid, the upper part of the buffer section (601a) is provided with a first valve body (603), and the lower part of the buffer section (601a) is provided with a second valve body (603). The exhaust pipe (602) is provided with a third valve body (605); the first valve body (603) is used to open when the waste liquid enters the liquid inlet pipe (601), and is closed after the waste liquid enters the buffer section (601a); the second valve body (604) and the third valve body (605) are both used to open after the waste liquid enters the buffer section (601a) and the first valve body (603) is closed, and are closed after the waste liquid enters the accommodating chamber, and the clamp (3) can rotate around a horizontal axis so that the sample in the culture dish is poured into the liquid inlet pipe (601).

2. A fully automatic chromosome preparation device according to claim 1, characterized in that: The second transfer assembly comprises a translation drive member (401) for pushing the mounting seat (4) to move in a lateral direction and a lifting drive member (402) for pushing the mounting seat (4) to move in a vertical direction.

3. A fully automatic chromosome preparation device according to claim 2, characterized in that: It also includes a barcode scanner (440) for identifying the culture dish, and the barcode scanner (440) is arranged toward the grasping component. The grasping component includes a first motor (411) and a suction cup (410) for holding the culture dish, and the first motor (411) is used to drive the suction cup (410) to rotate.

4. The fully automatic chromosome preparation device according to claim 2, characterized in that: The sample loading assembly comprises a sample loading needle (420), a sample loading rack (421) and a second motor (422); the sample loading rack (421) is provided with a plurality of slots (421a) for detachably connecting the sample loading needle (420); and the second motor (422) is used for driving the sample loading rack (421) to rotate.

5. The fully automatic chromosome preparation device according to claim 2, characterized in that: The rotary cover assembly comprises a buckle cover (430) and a third motor (432); a side of the buckle cover (430) facing the culture dish is provided with a rotary groove (431) matching the cover body of the culture dish; and the third motor (432) is used to drive the buckle cover (430) to rotate.

6. The fully automatic chromosome preparation device according to claim 1, characterized in that: The incubator (1) is provided with a turntable frame (110) for placing a culture dish, the turntable frame (110) is rotatably connected to the incubator (1), the turntable frame (110) is provided with a plurality of through grooves (111) arranged along the circumferential direction, the through grooves (111) all penetrate to the outer side of the turntable frame (110), the width of the through grooves (111) is smaller than the culture dish so that the culture dish can be placed above the through grooves (111), the side wall of the incubator (1) is provided with an operation window (101) for the clamping platform (2) to pass through, and the first transport component comprises a horizontal driving member (201) for pushing the clamping platform (2) into the incubator (1) and a vertical driving member (202) for pushing the clamping platform (2) to rise and fall.

7. The fully automatic chromosome preparation device according to claim 1, characterized in that: The centrifugal frame (5) comprises a main frame (510) connected to the centrifugal power source (501) and a plurality of sub-frames (520) for fixing culture dishes, wherein the sub-frames (520) are arranged along the circumference of the main frame (510), and the sub-frames (520) are rotatably connected to the main frame (510) and the rotation axis is arranged along a radial direction perpendicular to the main frame (510).

8. The fully automatic chromosome preparation device according to claim 7, characterized in that: The centrifugal mechanism further comprises an eccentric sleeve (530), an oscillating power source (531) and a sliding assembly (540); the sub-frame (520) comprises an outer ring frame (521) and an inner ring frame (522); the outer ring frame (521) is rotatably connected to the centrifugal frame (5) and the rotation axis is perpendicular to the radial direction of the centrifugal frame (5); the inner ring frame (522) is rotatably connected to the inner side of the outer ring frame (521) and the rotation axis is parallel to the radial direction of the centrifugal frame (5); the inner ring frame (522) is provided with a shelf for fixing a culture dish and a transmission rod (523) arranged in a vertical direction; the sliding assembly (540) is used to push the eccentric sleeve (530) to move vertically so that the lower end of the transmission rod (523) is inserted into the eccentric sleeve (530); and the oscillating power source (531) is used to drive the eccentric sleeve (530) to rotate.

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