Pipetting valve, sample processing device and molecular diagnostic system

Abstract

This application relates to the field of microfluidic control for in vitro diagnosis, and particularly to a pipetting valve, a sample processing device, and a molecular diagnostic system. The pipetting valve includes a first valve body, a second valve body, and a sealing member detachably connected to the second valve body. The second valve body is movably connected within the first valve body and is sealingly abutted against the first valve body through the sealing member. The first valve body forms a liquid extraction flow channel for communicating with a reagent cabin. The second valve body forms a liquid extraction port, and the sealing member forms a communication flow channel. One end of the communication flow channel is connected to the liquid extraction port. When the second valve body moves to a predetermined position, the other end of the communication flow channel can be connected to the liquid extraction flow channel so that the second valve body is connected to the reagent cabin. Since the sealing member is detachably connected to the second valve body, it not only saves production costs but also enables the sealing member to be replaced in size. While ensuring the sealing performance of the first valve body and the second valve body, the second valve body can be more easily driven to move within the first valve body.

Description

Technical Field

[0001] The present application relates to the field of microfluidic control for in vitro diagnosis, and particularly to a pipetting valve, a sample processing device, and a molecular diagnostic system. Background Art

[0002] In microfluidic control scenarios, such as in the field of in vitro diagnosis, pipetting valves are usually used to transfer samples or reagents. Existing pipetting valves generally include two valve bodies. One valve body forms multiple flow channels respectively communicating with multiple reagent chambers, and the other valve body is movably connected therein for reagent transfer; however, for the seal between the two valve bodies of existing pipetting valves, one is end face sealing, which requires high planar accuracy for the sealing end faces of the two valve bodies and has a large processing difficulty; the other is the method of encapsulating with rubber, encapsulating the sealing surface, which has high cost and low assembly efficiency. Summary of the Invention

[0003] The purpose of the present application is to provide a pipetting valve, a sample processing device, and a molecular diagnostic system to achieve the seal between the two valve bodies of the pipetting valve.

[0004] The present invention provides a pipetting valve, including a first valve body, a second valve body, and a sealing member; the second valve body is movably connected within the first valve body, the sealing member is detachably connected to the second valve body, and the second valve body is in sealing abutment with the first valve body through the sealing member; the second valve body forms a liquid taking port, the sealing member forms a communication flow channel, the first valve body forms a liquid taking flow channel, and as the second valve body moves within the first valve body, the sealing member moves synchronously with the second valve body, and enables the liquid taking port to communicate with the liquid taking flow channel through the communication flow channel.

[0005] Further, the number of the liquid taking flow channels is multiple, and the second valve body and the sealing member can move synchronously to enable the communication flow channel to be respectively connected with the multiple liquid taking flow channels.

[0006] Further, the multiple liquid taking flow channels are spaced apart along the circumferential direction, and the second valve body is rotatably connected within the first valve body.

[0007] Further, an annular groove is formed on the inner wall of the first valve body; a limiting boss is formed on the outer wall of the second valve body, and the limiting boss is inserted into the annular groove.

[0008] Further, the pipetting valve further includes a piston rod formed with a piston head; the piston head of the piston rod is located within the second valve body, and the piston head is in sealing abutment with the second valve body.

[0009] Furthermore, a sealing ring groove is formed on the side wall of the piston head, and a sealing ring is arranged in the sealing ring groove.

[0010] Furthermore, the sealing component is clamped on the second valve body.

[0011] Furthermore, the sealing component is gear-shaped; the liquid intake port is located on the bottom wall of the second valve body, the bottom wall of the second valve body forms a plug-in groove, and the plug-in groove is adapted to the sealing component so that the sealing component can be plugged into the plug-in groove.

[0012] Furthermore, a flattening axis is formed on the side of the sealing component facing the second valve body; a flattening groove is formed on the bottom wall of the second valve body, and the flattening axis is inserted into the flattening groove.

[0013] Furthermore, the sealing component is formed of a sealing rubber material; and an adhesive layer is provided between the sealing component and the second valve body.

[0014] Further, the connecting flow channel includes a connecting hole and a guide groove; the connecting hole passes through the sealing component, and the connecting hole is connected to the liquid intake port; the guide groove is located on the side of the sealing component away from the second valve body, and the guide groove extends along the radial direction of the sealing component, one end of the guide groove is connected to the liquid intake port through the connecting hole, and the other end of the guide groove can be connected to the liquid intake flow channel.

[0015] The present invention also provides a sample processing device, comprising any one of the above-mentioned liquid transfer valves.

[0016] The present invention also provides a molecular diagnostic system, comprising any one of the sample processing devices described above.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The pipetting valve provided by the present invention includes a first valve body, a second valve body, and a sealing member; the second valve body is formed with a liquid extraction port communicating with the valve cavity of the second valve body, the first valve body forms a liquid extraction flow channel, and the liquid extraction flow channel is used to communicate with a reagent cabin containing reagents; one end of the second valve body formed with the liquid extraction port is movably connected within the first valve body, and the second valve body can move within the first valve body to connect the liquid extraction port of the second valve body with the liquid extraction flow channel of the second valve body, thereby connecting the second valve body with the reagent cabin; the sealing member can move synchronously with the second valve body, the sealing member is formed with a communicating flow channel, and one end of the communicating flow channel is connected to the liquid extraction port of the second valve body. When the second valve body is installed within the first valve body, the sealing member can be clamped between the first valve body and the second valve body, so that the first valve body and the second valve body are in sealed abutment through the sealing member; when the second valve body moves to a predetermined position, the end of the communicating flow channel away from the liquid extraction port can be connected to the liquid extraction flow channel of the first valve body, so that the second valve body is connected to the corresponding reagent cabin containing reagents through the communicating flow channel on the sealing member and the liquid extraction flow channel of the first valve body; thereby, the first valve body and the second valve body are sealed through the sealing member, and the second valve body is connected to the communicating flow channel on the first valve body to extract the reagent in the reagent cabin into the second valve body, or to transport the reagent in the second valve body to the connected reagent cabin; at the same time, since the sealing member is detachably connected to the second valve body, compared with the existing sealing methods, such as end face sealing between the first valve body and the second valve body, or encapsulating the second valve body with rubber, detachably connecting the sealing member to the second valve body not only saves production costs, but also enables the sealing member to be replaced in size. While ensuring the sealing between the first valve body and the second valve body, the second valve body can be more easily driven to move within the first valve body.

[0019] The present invention also provides a sample processing device, including the pipetting valve described above, so the sample processing device also has the beneficial effects of the pipetting valve.

[0020] The present invention also provides a molecular diagnosis system, including the sample processing device described above, so the molecular diagnosis system also has the beneficial effects of the sample processing device. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required to be used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as limiting the scope. For those of ordinary skill in the art, other related drawings can be obtained based on these drawings without creative efforts.

[0022] Figure 1 It is a schematic diagram of the split structure of the pipetting valve provided by the embodiment of the present invention;

[0023] Figure 2Schematic cross-sectional structure diagram of the pipetting valve provided by the embodiment of the present invention;

[0024] Figure 3 Schematic structure diagram of the second valve body of the pipetting valve provided by the embodiment of the present invention;

[0025] Figure 4 Schematic structure diagram of the sealing glue of the pipetting valve provided by the embodiment of the present invention from the first perspective;

[0026] Figure 5 Schematic structure diagram of the sealing glue of the pipetting valve provided by the embodiment of the present invention from the second perspective.

[0027] Reference numerals:

[0028] 1 - First valve body, 11 - Liquid extraction flow channel, 12 - Annular groove, 2 - Second valve body, 21 - Liquid extraction port, 22 - Limit boss, 23 - Insertion groove, 24 - Flat position groove, 3 - Sealing glue, 31 - Communication flow channel, 32 - Flat position shaft, 33 - Communication hole, 34 - Diversion groove, 4 - Piston rod, 41 - Sealing ring. Detailed implementation manners

[0029] Next, the technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention.

[0030] Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed present invention, but merely represents the selected embodiments of the present invention.

[0031] All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0032] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be understood as a limitation of the present invention. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0033] In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, the terms "installation", "connection", and "coupling" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0034] Next, refer to Figures 1 to 5 to describe a pipetting valve, a sample processing device, and a molecular diagnostic system according to some embodiments of the present application.

[0035] The present application provides a pipetting valve. As Figure 1 and Figure 2 shown, the pipetting valve includes a first valve body 1, a second valve body 2, a piston rod 4, and a sealing member. In the embodiment, the sealing member is a sealing glue 3. The second valve body 2 is formed with a liquid extraction port 21 communicating with the valve cavity of the second valve body 2. One end of the piston rod 4 is located in the valve cavity of the second valve body 2. The end of the piston rod 4 located in the second valve body 2 forms a piston head, and the piston head is in sealing abutment with the valve cavity of the second valve body 2. The piston rod 4 can reciprocate in the second valve body 2 along its own axis direction. Pushing the piston rod 4 into the second valve body 2 can discharge the reagent in the second valve body 2 through the liquid extraction port 21 of the second valve body 2. Pulling the piston rod 4 out of the second valve body 2 can extract the reagent into the second valve body 2, so that the reagent enters the second valve body 2 through the liquid extraction port 21. In the embodiment, the sealing glue 3 is formed of a sealing rubber material.

[0036] As Figure 1 and Figure 2 shown, the first valve body 1 forms a liquid extraction flow channel 11. The liquid extraction flow channel 11 is used to communicate with a reagent cabin containing a reagent. One end of the second valve body 2 formed with the liquid extraction port 21 is movably connected inside the first valve body 1. The second valve body 2 can move inside the first valve body 1 to connect the liquid extraction port 21 of the second valve body 2 with the liquid extraction flow channel 11 of the second valve body 2, and further connect the second valve body 2 with the reagent cabin. Thus, when the operator pulls the piston rod 4 out of the second valve body 2, the reagent in the reagent cabin can be extracted into the second valve body 2. When the operator pushes the piston rod 4 into the second valve body 2, the reagent in the second valve body 2 can be transported into the reagent cabin.

[0037] Preferably, as Figures 1 to 3As shown, one end of the second valve body 2 located inside the first valve body 1 forms a bottom wall (i.e., the bottom wall of the second valve body 2), and a liquid extraction port 21 is formed on the bottom wall of the second valve body 2. The first valve body 1 forms a bottom wall (i.e., the bottom wall of the first valve body 1), and a liquid extraction flow channel 11 is formed on the bottom wall of the first valve body 1. A sealing rubber 3 is clamped on the bottom wall of the second valve body 2. When the second valve body 2 moves inside the first valve body 1, the sealing rubber 3 can move synchronously with the second valve body 2. The sealing rubber 3 forms a communication flow channel 31, and one end of the communication flow channel 31 is connected to the liquid extraction port 21 of the second valve body 2. When the second valve body 2 is installed inside the first valve body 1, the sealing rubber 3 can be clamped between the bottom wall of the first valve body 1 and the bottom wall of the second valve body 2, so that the first valve body 1 and the second valve body 2 are sealed and abutted against each other through the sealing rubber 3. When the second valve body 2 moves to a predetermined position, the end of the communication flow channel 31 away from the liquid extraction port 21 can be connected to the liquid extraction flow channel 11 of the first valve body 1, so that the second valve body 2 is connected to the corresponding reagent cabin filled with reagents through the communication flow channel 31 on the sealing rubber 3 and the liquid extraction flow channel 11 of the first valve body 1. Thus, the first valve body 1 and the second valve body 2 are sealed through the sealing rubber 3, and the communication flow channel 31 on the second valve body 2 is connected to the first valve body 1, so as to extract the reagent in the reagent cabin into the second valve body 2, or transport the reagent in the second valve body 2 to the corresponding connected reagent cabin. At the same time, since the sealing rubber 3 and the second valve body 2 are detachably connected, compared with the existing sealing methods, such as end face sealing between the first valve body 1 and the second valve body 2, or encapsulating the second valve body 2, connecting the sealing rubber 3 detachably to the second valve body 2 not only saves production costs, but also enables the sealing rubber 3 to be replaced in size. While ensuring the sealing performance between the first valve body 1 and the second valve body 2, the second valve body 2 can be more easily driven to rotate inside the first valve body 1.

[0038] In this embodiment, preferably, as Figure 1 shown, the number of the liquid extraction flow channels 11 of the first valve body 1 is multiple, the multiple liquid extraction flow channels 11 are spaced apart, and the multiple liquid extraction flow channels 11 are respectively connected to different reagent cabins. The second valve body 2 can move to different positions inside the first valve body 1, so that the liquid extraction port 21 of the second valve body 2 is respectively connected to the multiple liquid extraction flow channels 11 through the communication flow channel 31 of the sealing rubber 3, and further enables the reagent cabin corresponding to the liquid extraction flow channel 11 to be connected to the liquid extraction port 21 of the second valve body 2.

[0039] In this embodiment, preferably, as Figure 1As shown, a plurality of liquid extraction channels 11 of the first valve body 1 are circumferentially and spaced apart. The second valve body 2 is rotatably connected within the first valve body 1. When the second valve body 2 rotates, the sealing glue 3 can rotate synchronously with the second valve body 2. And when the second valve body 2 rotates to different positions, the liquid extraction port 21 can be respectively communicated with a plurality of liquid extraction channels 11 and the reagent compartments corresponding to the liquid extraction channels 11 through the communication channels 31, so that the second valve body 2 is respectively communicated with a plurality of reagent compartments. Furthermore, reagent transfer can be performed between a plurality of reagent compartments through the second valve body 2.

[0040] In this embodiment, preferably, as Figure 4 and Figure 5 shown, the communication channels 31 on the sealing glue 3 include communication holes 33 and diversion grooves 34. The communication holes 33 penetrate through the sealing glue 3, and the communication holes 33 are directly opposite to the liquid extraction ports 21 on the bottom wall of the second valve body 2, so that the communication holes 33 are communicated with the liquid extraction ports 21. One end of the diversion groove 34 is communicated with the communication hole 33, and the other end of the diversion groove 34 extends along the radial direction of the bottom wall of the second valve body 2. It should be noted that the second valve body 2 and the second valve body 2 are coaxially arranged. The liquid extraction port 21 of the second valve body 2 is located at the axis center of the bottom wall of the second valve body 2. A plurality of liquid extraction channels 11 of the first valve body 1 are circumferentially and spaced apart around the axis of the first valve body 1. The distance from the end of the diversion groove 34 far from the axis of the second valve body 2 to the second axis is the same as the distance from the end of the communication channel 31 facing the first axis to the first axis. Thus, when the second valve body 2 and the sealing glue 3 rotate to different predetermined angles, the other end of the diversion groove 34 can be respectively communicated with a plurality of liquid extraction channels 11.

[0041] During specific operation, when reagent transfer is required between two reagent compartments (the first reagent compartment and the second reagent compartment), for example, when it is necessary to transfer the reagent in the first reagent compartment to the second reagent compartment, one of the plurality of liquid extraction channels 11 of the first valve body 1 is communicated with the first reagent compartment, and the other liquid extraction channel 11 is communicated with the second reagent compartment. Rotating the second valve body 2 can make the liquid extraction port 21 of the second valve body 2 be respectively communicated with the liquid extraction channel 11 and the corresponding first reagent compartment or second reagent compartment of the liquid extraction channel 11 through the communication channel 31 of the sealing glue 3. First, rotate the second valve body 2, and the sealing glue 3 rotates synchronously with the second valve body 2 to make the second valve body 2 rotate to a position communicated with the first reagent compartment. Pull the piston rod 4, and the reagent in the first reagent compartment will be extracted into the second valve body 2. Then rotate the second valve body 2 again to make the second valve body 2 rotate to a position communicated with the second reagent compartment. Push the piston rod 4 into the second valve body 2, and the reagent in the second valve body 2 will be pushed into the second reagent compartment. Thus, through the second valve body 2 as a transfer station, reagent transfer can be performed between a plurality of reagent compartments communicated with a plurality of liquid extraction channels 11 of the first valve body 1.

[0042] In an embodiment of the present application, preferably, asFigures 2 to 5 As shown, the sealant 3 is in a gear shape. An insertion groove 23 is formed on the bottom wall of the second valve body 2. The insertion groove 23 is adapted to the sealant 3, enabling the sealant 3 to be stably and firmly inserted and installed in the insertion groove 23. When the second valve body 2 rotates, the sealant 3 can rotate synchronously with the second valve body 2. At the same time, when the sealant 3 is inserted and installed in the insertion groove 23, the end face of the sealant 3 protrudes from the insertion groove 23. When the second valve body 2 is installed in the first valve body 1, the sealant 3 will be compressed and clamped between the bottom wall of the first valve body 1 and the bottom wall of the second valve body 2, thereby playing a sealing role.

[0043] In this embodiment, an anti-fooling positioning structure is provided for the installation between the sealant 3 and the second valve body 2. Preferably, as Figure 4 shown, a flat shaft 32 is formed at one end of the sealant 3 facing the second valve body 2. A flat groove 24 is formed on the bottom wall of the insertion groove 23. The flat groove 24 is adapted to the flat shaft 32, enabling the flat shaft 32 to be inserted into the flat groove 24, thereby positioning the installation between the sealant 3 and the second valve body 2.

[0044] In this embodiment, preferably, a bonding layer is provided between the sealant 3 and the second valve body 2, so that the sealant 3 is more stably and firmly installed on the second valve body 2, enabling the sealant 3 to rotate synchronously with the second valve body 2, and playing a role in sealing and connecting the second valve body 2 and the liquid extraction channel 11.

[0045] In an embodiment of the present application, preferably, as Figure 2 shown, a piston head is formed at one end of the piston rod 4 located inside the second valve body 2. A sealing ring groove is formed on the side wall of the piston head, and a sealing ring 41 is installed in the sealing ring groove, enabling the piston head to be in sealing contact with the inner wall of the second valve body 2. Thus, when the piston rod 4 is pulled outwards, a suction force can be formed to extract the reagent into the second valve body 2. When the piston rod 4 is pushed into the second valve body 2, the reagent inside the second valve body 2 can be conveyed out.

[0046] In an embodiment of the present application, preferably, as Figure 2 shown, an annular groove 12 is formed on the inner wall of the first valve body 1, and a limiting boss 22 is formed on the outer wall of the second valve body 2. When the second valve body 2 is installed in the first valve body 1, the limiting boss 22 of the second valve body 2 can be clamped in the annular groove 12 of the first valve body 1, thereby restricting the axial movement of the second valve body 2, enabling the second valve body 2 to only rotate around its own axis inside the first valve body 1. And when the second valve body 2 is clamped in the first valve body 1, the distance between the second valve body 2 and the bottom wall of the first valve body 1 can compress the sealant 3 therebetween, thereby playing a better sealing role.

[0047] The present application also provides a sample processing device, including the pipetting valve of any of the above embodiments.

[0048] In this embodiment, the sample processing device includes a pipetting valve. The sample processing device is used for nucleic acid extraction of samples. During the sample processing, various reagents for sample processing are transferred through the pipetting valve, so that the samples placed in the sample processing device react with the reagents to perform nucleic acid extraction on the samples. Therefore, the sample processing device has all the beneficial effects of the pipetting valve, which will not be elaborated here one by one.

[0049] The present application also provides a molecular diagnostic system, including the sample processing device of the above embodiment.

[0050] In this embodiment, the molecular diagnostic system includes a sample processing device. The molecular diagnostic system is used to complete nucleic acid detection of samples such as cells, hair, anticoagulated blood, or dried blood stains of the person to be tested at the molecular level. The sample completes nucleic acid extraction in the sample processing device, and then completes nucleic acid detection of the sample through subsequent nucleic acid molecular hybridization, polymerase chain reaction (PCR), and biochip and other detection steps in the molecular diagnostic system, so as to accurately perform detection once the disease occurs or before symptoms, signs, and biochemical changes appear. The molecular diagnostic system includes a sample processing device. Therefore, the molecular diagnostic system has all the beneficial effects of the sample processing device, which will not be elaborated here one by one again.

[0051] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A pipetting valve, characterized in that, comprising a first valve body, a second valve body and a sealing member; The second valve body is movably connected to the first valve body, the sealing member is detachably connected to the second valve body, and the second valve body is sealed against the first valve body through the sealing member; The second valve body forms a liquid intake port, the sealing member forms a communication channel, and the first valve body forms a liquid intake channel. As the second valve body moves in the first valve body, the sealing member moves synchronously with the second valve body, and enables the liquid intake port to communicate with the liquid intake channel via the communication channel. An annular groove is formed on the inner wall of the first valve body; a limiting boss is formed on the outer wall of the second valve body, and the limiting boss is inserted into the annular groove; When the second valve body is clamped in the first valve body, the distance between the second valve body and the bottom wall of the first valve body can compress the sealing component therebetween.

2. The pipetting valve according to claim 1, wherein The number of the liquid intake flow channels is multiple, and the second valve body and the sealing member can move synchronously to make the communication flow channel communicate with the multiple liquid intake flow channels respectively.

3. The pipetting valve according to claim 2, characterized in that, The plurality of liquid extraction channels are spaced apart and distributed along the circumferential direction, and the second valve body is rotatably connected to the first valve body.

4. The pipetting valve according to any one of claims 1 to 3, characterized in that, Also included is a piston rod formed with a piston head; The piston head of the piston rod is located in the second valve body, and the piston head is in sealing contact with the second valve body.

5. The pipetting valve according to any one of claims 1 to 3, characterized in that, The sealing component is clamped to the second valve body.

6. The pipetting valve according to claim 5, characterized in that The sealing member is gear-shaped; The liquid taking port is located on the bottom wall of the second valve body. The bottom wall of the second valve body forms an inserting groove. The inserting groove is adapted to the sealing component so that the sealing component is inserted into the inserting groove.

7. The pipetting valve according to claim 5, characterized in that, The side of the sealing member facing the second valve body is formed with a flattened axis; The bottom wall of the second valve body forms a flat groove, and the flat shaft is inserted into the flat groove.

8. The pipetting valve according to any one of claims 1 to 3, characterized in that, The sealing member is formed of a sealing rubber material; An adhesive layer is provided between the sealing component and the second valve body.

9. The pipetting valve according to any one of claims 1 to 3, characterized in that, The communication channel includes a communication hole and a guide groove; The communicating hole penetrates the sealing member, and the communicating hole is connected with the liquid taking port; The guide groove is located on a side of the sealing component away from the second valve body, and extends radially along the sealing component. One end of the guide groove is connected to the liquid intake port through the connecting hole, and the other end of the guide groove can be connected to the liquid intake channel.

10. A sample processing device, characterized in that, A pipetting valve comprising the pipetting valve according to any one of claims 1 to 9.

11. A molecular diagnostic system, characterized in that, The sample processing device according to claim 10 is included.

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