Cosmetic composition comprising a combination of at least one oligosaccharide and / or polysaccharide in combination with mannose monosaccharide and its use in maintaining the balance of the bacterial flora of the skin

Abstract

The present invention relates to a cosmetic composition for topical application, comprising in a physiologically acceptable medium: at least one oligosaccharide and / or polysaccharide selected from the group consisting of inulin, oligofructose, oligoglucose, soy derived oligosaccharides, pyrodextrins, isomaltooligosaccharides, oligoxyloses, transgalacto-oligosaccharides and mixtures thereof, and at least one mannose monosaccharide. The present invention also relates to a cosmetic treatment method for caring for the skin and / or mucous membranes, comprising applying the composition to the skin and / or the mucous membranes, in particular having been subjected to external aggression; and also to its cosmetic use.

Description

[0001] The present invention relates to a cosmetic composition for topical application, comprising a combination of at least one oligosaccharide and / or polysaccharide in combination with mannose monosaccharides in a physiologically acceptable medium. The invention also relates to the use and method of cosmetic treatment using this combination to maintain and / or restore the balance of the bacterial flora of the skin and / or mucous membranes.

[0002] Human skin consists of two parts, the deep part (dermis) and the surface part (epidermis).

[0003] The skin plays an important role in protecting against external aggressions such as environmental aggressions, climatic stress (heat, cold, UV, tobacco, etc.), pollution, allergens, pathogenic germs, mechanical aggressions (hair removal, shaving, abrasive scrubbing) and chemical aggressions (detergents). This property, called barrier function, is mainly performed by the outermost layer of the epidermis, the stratum corneum.

[0004] The skin also represents a complex ecosystem on which several types of microorganisms, such as bacteria and fungi, proliferate. These microorganisms constitute the skin flora, also known as the skin microbiome.

[0005] To date, more than 500 bacterial species have been detected on healthy skin, containing more than 2 million genes. 2 The skin is inhabited by about 10 6 The skin microbiome of healthy subjects has been described as being specific according to 3 regions: moist, dry, and oily.

[0006] The special distinctions are as follows:

[0007] - The commensal beneficial resident flora consists of microorganisms that routinely and permanently proliferate on healthy skin, draw their nutrients from the skin and provide known benefits to the skin; a particular representative is the strain of Staphylococcus epidermidis. This strain is particularly present on the surface of healthy skin, where it participates in maintaining the balance of the commensal skin flora.

[0008] - Transient flora is present on the skin under abnormal conditions, for example through contact with contaminated elements; if this flora proliferates, it can become pathogenic. A possible example is Staphylococcus aureus, which is potentially pathogenic to human skin.

[0009] In cases where the barrier is adversely affected or when the balance between commensals and pathogens is disturbed, for example after an external aggression, this may result in deterioration of skin quality and / or skin diseases, for example cases of irritation, stinging, pulling, redness, itching, or even skin diseases such as atopic dermatitis, acne or skin infections (Functions of the skin microbiota in health and disease. James A. Sanford, Richard L. Gallo. Seminars in Immunology 25 (2013) 370-377; The human skin microbiome. Allyson L. Byrd, Yasmine Belkaid and Julia A. Segre. Nature Reviews Microbiology, 16, 143-155, 2018).

[0010] Furthermore, it is obvious that the quality of the skin barrier and mucous membranes is affected every day by external aggressions from irritants (detergents, surfactants, acids, alkalis, oxidizing agents, reducing agents, concentrated solvents), mechanical stress (friction, scrubbing, shaving or hair removal) or thermal or climatic imbalances (cold, dryness).

[0011] Therefore, the (re)colonization of the skin by beneficial microorganisms of the commensal skin flora appears to be fundamental to skin physiology and immunity, especially due to their effects on [1]-[9] :

[0012] - Limiting the adhesion of pathogenic microorganisms (either by stimulating antimicrobial peptides or by competitive adhesion);

[0013] - Regulation of inflammation and immunity;

[0014] -heal;

[0015] - Restoration / reinstatement of barrier function, especially after external insults as described above.

[0016] There are already known cosmetic solutions for acting on the skin microflora.

[0017] US 20180325968 specifically describes a product comprising a composition of prebiotic lipids and a composition containing probiotics (such as Bacillus licheniformis, Bifidobacterium breve, Bifidobacterium infantis, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus sakei, Lactobacillus pseudocasei, Staphylococcus epidermidis and Staphylococcus xylosus) for correcting dysbiosis (colonization of pathogenic microorganisms) or maintaining the balance of resident beneficial commensal flora.

[0018] Furthermore, FR 3064473 discloses the use of pullulan or its derivatives or a combination thereof with a polysaccharide chosen from hyaluronic acid or one of its salts or derivatives, alginic acid, one of its salts or derivatives, and mixtures of these polysaccharides for maintaining and / or preserving the balance of the microflora of the skin and / or mucous membranes.

[0019] CN 107412045 discloses a prebiotic skin care mask, which particularly comprises α-oligoglucose, inulin, yeast and Lactobacillus bacteria, for strengthening the presence of healthy skin flora and preventing pathogenic bacteria from colonizing on the skin.

[0020] However, the ever-increasing demand of consumers for treatments that respect their skin and especially their skin flora has led to a search for new compounds active in maintaining or restoring the balance of the cutaneous and / or mucosal bacterial flora.

[0021] There is therefore a need to provide new ingredients which are active on the skin microflora, in particular by protecting the commensal flora, and which are able to maintain and / or restore the balance of the skin and / or mucosal bacterial flora.

[0022] Unexpectedly, the inventors have discovered that a combination of oligosaccharides and / or polysaccharides selected from the group consisting of inulin, fructooligosaccharides, glucosamine, soy-derived oligosaccharides, pyrodextrins, isomaltooligosaccharides, xylooligosaccharides, transgalacto-oligosaccharides and mixtures thereof, and mannose monosaccharides have the ability to maintain and / or restore the balance of the skin and / or mucosal microflora, in particular by restoring an appropriate number of bacteria on the skin (i.e., bacterial load) and the diversity of bacteria on the skin (i.e., α and β diversity), both parameters being reduced in particular in the event of external aggressions.

[0023] Furthermore, the combination makes it possible to prevent and / or treat impairments of the aesthetic qualities of the skin and / or mucous membranes, in particular to prevent and / or treat dry and / or rough skin.

[0024] "Impairment of the aesthetic qualities of the skin" refers to all changes to the skin that impair the aesthetic appearance of the skin, in particular the appearance of a rough texture of the facial skin, in particular the cheeks and / or forehead, and / or the presence of signs of dry skin.

[0025] Furthermore, the combination is able to prevent and / or treat sensitive skin, in particular to prevent and / or treat uncomfortable sensations of sensitive skin, such as, for example, a sensation of skin pulling.

[0026] The subject of the present invention is therefore a cosmetic composition for topical application comprising, in a physiologically acceptable medium:

[0027] - at least one oligosaccharide and / or polysaccharide selected from the group consisting of inulin, fructooligosaccharides, glucooligosaccharides, soy derived oligosaccharides, pyrodextrins, isomaltooligosaccharides, xylooligosaccharides, transgalacto-oligosaccharides and mixtures thereof, and

[0028] - At least one mannose monosaccharide.

[0029] In particular, said oligosaccharides and / or polysaccharides and said mannose monosaccharide are prebiotics.

[0030] The invention also relates to a cosmetic treatment method for caring for the skin and / or mucous membranes, which method comprises applying a composition according to the invention to the skin and / or mucous membranes, in particular which have been subjected to external aggressions.

[0031] In particular, the cosmetic treatment method according to the invention makes it possible to maintain and / or restore the balance of the bacterial flora of the skin and / or mucous membranes.

[0032] Furthermore, the cosmetic treatment method according to the invention makes it possible to prevent and / or treat impairments of the aesthetic qualities of the skin and / or mucous membranes, in particular to prevent and / or treat dry and / or rough skin.

[0033] The cosmetic treatment method according to the invention also makes it possible to prevent and / or treat sensitive skin, in particular to prevent and / or treat discomforts of sensitive skin.

[0034] The invention also relates to the topical cosmetic use of said combination and optionally at least one probiotic microorganism for caring for the skin, in particular for skin that has been subjected to external aggressions.

[0035] The invention also relates to the use of said combination for maintaining and / or restoring the balance of the bacterial flora of the skin and / or mucous membranes.

[0036] Furthermore, the present invention relates to the use of said combination for preventing and / or treating sensitive skin, in particular for preventing and / or treating discomfort of sensitive skin.

[0037] Finally, the invention relates to the use of said combination for preventing and / or treating impairments of the aesthetic qualities of the skin and / or mucous membranes, in particular for preventing and / or treating dry and / or rough skin.

[0038] definition

[0039] The term "prebiotic" refers to a substrate that is selectively used by host microorganisms to produce a health benefit [Gibson, Glenn R.; Hutkins, Robert; Sanders, Mary Ellen; Prescott, Susan L.; Reimer, Raylene A.; Salminen, Seppo J.; Scott, Karen; Stanton, Catherine; Swanson, Kelly S.; Cani, Patrice D.; Verbeke, Kristin; Reid, Gregor (2017). "Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics." Nature Reviews Gastroenterology & Hepatology. 14(8): 491-502.].

[0040] Prebiotics are particularly useful for maintaining an environment conducive to the development of beneficial endogenous skin flora. Prebiotics are particularly useful for directing the metabolism of the entire skin flora so as to maintain a good balance of the flora without promoting the development of certain pathogenic bacteria. For this reason, the prebiotics used in the context of the present invention are those that can be metabolized by the beneficial bacteria of the skin flora. According to a particularly preferred embodiment, the prebiotic used in the composition of the present invention is a prebiotic that is metabolized by the beneficial bacteria of the skin, but cannot be metabolized by undesirable bacteria that cause dermatoses that require cosmetic treatment. According to this embodiment, prebiotics regulate the composition and / or activity of the natural ecosystem by promoting those microorganisms that play a positive role on the skin.

[0041] Oligosaccharides and polysaccharides are carbohydrates.

[0042] The oligosaccharides and / or polysaccharides may in particular be produced from glucose, galactose, xylose, maltose, sucrose, lactose, starch, xylan, hemicellulose, inulin, gums (in particular gum arabic), or mixtures thereof.

[0043] The term "oligosaccharide" is intended to mean oligoholosides or, alternatively, oligosides, which are oligomers composed of n monosaccharides via one or more α or β glycosidic bonds, the number of n being between 2 and 10, preferably between 2 and 6. Mention may be made in particular of fructooligosaccharides, glucooligosaccharides, oligosaccharides derived from soy, pyrodextrins, isomaltooligosaccharides, xylo-oligosaccharides and transgalacto-oligosaccharides.

[0044] The term "polysaccharide" is intended to mean polyosides, polyholosides or complex carbohydrates, which are polymers composed of n units of monosaccharides linked together by sugar bonds, the number of n being greater than or equal to 11, preferably ranging from 11 to 200, even better still from 11 to 100, even more preferably from 20 to 80. Mention may especially be made of inulin.

[0045] The expression "composition comprising a physiologically acceptable medium" is intended to mean a composition comprising a medium that is compatible with the skin.

[0046] According to a particular embodiment, the pH of the cosmetic composition is between 4 and 7.5, in particular between 4.5 and 7, and in particular between 4.7 and 6.

[0047] The term "skin" is intended to mean the entire skin of the human body, and preferably the skin of the face, the skin of the décolleté, neck, arms and forearms, or even more preferably the skin of the face, especially the forehead, nose, cheeks, chin and periorbital area.

[0048] According to the present invention, the term "preventing" or "prevention" is intended to mean reducing the risk of occurrence of a given phenomenon or slowing down the occurrence of a given phenomenon.

[0049] The term "treating" or "treatment" is intended to refer to any action intended to improve a person's comfort, well-being, or happiness; thus, the term encompasses attenuation, relief, and cure.

[0050] In the sense of the present invention, the term "external aggressions" refers to environmental aggressions, climatic aggressions (heat, cold, UV, tobacco, etc.), pollution, allergens, pathogenic germs, mechanical stress (hair removal, shaving, abrasive scrubbing) and chemical aggressions (detergents).

[0051] The term "bacterial load" refers to the number of bacteria, particularly the amount of bacterial DNA found on the skin and / or mucous membranes.

[0052] The term “relative abundance” refers to the proportion of each genus / bacteria in the studied sample or population.

[0053] The term "alpha diversity" or "Shannon index" refers to the number of different bacterial species / genera in a study sample or population; the greater the number of different bacterial species / genera, the richer and more diverse the sample or population.

[0054] In particular, the Shannon index is calculated for each studied sample or population and is based on three different parameters:

[0055] - Number of different bacterial species / genera;

[0056] - the incidence of the bacterium under consideration in the study sample or population, in particular whether the bacterium is present in all individuals in the sample or population or only in a minority of individuals;

[0057] -The weight of each bacterial species / genus identified in the study sample or population, calculated taking into account the relative abundance defined above.

[0058] The term "beta diversity" refers to the number of different bacterial species / genera between two samples or populations, in other words, the number of bacterial species / genera that the two samples or populations do not have in common. Therefore, in contrast to alpha diversity, beta diversity is calculated based on two samples or populations.

[0059] Beta diversity is based on three different parameters (e.g. UniFrac distance):

[0060] - Number of different bacterial species / genera;

[0061] - their relative abundance (i.e., the weight of each bacterial species / genus);

[0062] - Phylogenetic distinctions between these bacterial species / genera. DETAILED DESCRIPTION

[0063] Oligosaccharides, polysaccharides

[0064] The composition according to the present invention comprises at least one oligosaccharide and / or polysaccharide selected from the group consisting of inulin, fructooligosaccharides, glucooligosaccharides, soy derived oligosaccharides, pyrodextrins, isomaltooligosaccharides, xylo-oligosaccharides, trans-galacto-oligosaccharides and mixtures thereof.

[0065] By way of example, the following oligosaccharides and / or polysaccharides will be mentioned.

[0066] Inulin

[0067] Inulin is particularly abundant in the rhizomes of plants (especially Jerusalem artichokes and chicory), from which it can be extracted industrially. It is also found in other plants belonging to the Asteraceae family (such as Jerusalem artichokes or dahlia onions and burdock). It is considered a soluble dietary fiber.

[0068] [Chemical formula 1]

[0069]

[0070] Inulin is a polydisperse linear polymer of general formula (I): GFn (G = glucose, F = fructose, n ranging from 2 to more than 60), the fructose units being linked together by β (2→1) bonds. Inulin thus corresponds to a chain of fructose units terminated by a glucose unit.

[0071] Among the inulins that can be used and are commercially available, mention may be made of Inutec H25P, with n between 2 and 7, and Inutec N25 (average n=25) from the company Orafti.

[0072] Fructo-oligosaccharides

[0073] Fructooligosaccharides (or FOS), also known as fructooligosaccharides or oligofructans, are short chains of fructose linked together by beta-1,2 bonds.

[0074] Fructo-oligosaccharides (or FOS) correspond to the general formula (II): G(F) n , wherein G is a glucose unit, F is a fructose unit and n ranges from 1 to 10.

[0075] [Chemical formula 2]

[0076]

[0077] Production of FOS:

[0078] - Through inulin (e.g. from Orafti, Belgium ), or

[0079] - by using sucrose (e.g. Beghin Meiiji Industries from France) ) for enzyme synthesis, or

[0080] - by extraction from yacon or jicama (Polymnia sonchifolia, synonym: Smallanthus sonchifolia); in particular, by cold pressing of yacon tubers in the absence of solvents.

[0081] The FOS used in the present invention may be a mixture of FOS. Particular mention may be made of a mixture of GF2+GF3+GF4, such as Quantom FOS95 from Quantum hi-Tech or FOS95 from BeghinMeiiji Industrial Company of France. The latter corresponds to a mixture of 37% GF2, 53% GF3 and 10% GF4.

[0082] Glucose oligosaccharide GOS

[0083] Glucose oligosaccharides (GOS), or oligoglucans, are oligosaccharides composed of a series of α-1,6-linked glucose units that may also contain α-1,2; α-1,3; α-1,4 bonds. They are synthesized by transglycosylation catalyzed by enzymes of the glucan-sucrase family.

[0084] Preferably, the oligoglucose is an oligosaccharide consisting of a series of α-1,6- and α-1,2-linked glucose units.

[0085] Advantageously, the number of glucose units is between 2 and 10, even better still between 4 and 6, even more preferably the number of glucose units is 4.

[0086] Furthermore, oligoglucose can be synthesized by polymerization of glucose molecules (a reaction catalyzed by specific enzymes of the glycosyltransferase type) and extracted and purified from the bacterial strain of Leuconostoc mesenteroides. This reaction requires the use of an acceptor: maltose (glucose-glucose) and a glucose donor: sucrose (glucose-fructose).

[0087] In a preferred embodiment, the oligosaccharide (GOS) has the following formula (III):

[0088] [Chemical formula 3]

[0089]

[0090] Among the GOS that can be used and are commercially available, mention may be made of GOS from Solabia.

[0091] Soy-derived oligosaccharides

[0092] They are directly extracted from soybeans and do not require any enzyme treatment. They naturally contain raffinose and stachyose, and their formula is [α-D-Gal-(1→6)-] m -α-D-Glu-(1→2)-β-D-Fru, wherein for raffinose, m=1 and for stachyose, m=2.

[0093] Derivatized oligosaccharides that may be used include Soya-oligo from Calpis Food Ind. of Japan.

[0094] Pyrodextrin

[0095] Dextrin is a mixture of oligosaccharides resulting from the hydrolysis of starch.

[0096] Isomaltooligosaccharide

[0097] They are produced from starch. These are α-(1,6)-linked glucose oligomers with a degree of polymerization between 2 and 5. For example, Isomalto900P from Showa Sango Co., Ltd. can be used.

[0098] Xylo-oligosaccharide

[0099] Xylo-oligosaccharide is an oligosaccharide composed of β-(1,4)-linked xylose. For example, Xylo 95P from Suntory limited can be used.

[0100] Trans-Galacto-oligosaccharide

[0101] These are linear oligomers of galactose with the chemical structure α-D-glucose-(1→4)-[β-D-galactose-(1→6)-]n (2 < n < 5), obtained by lactose fermentation. For example, TOS 100 from Yakult Honsha Co., Ltd. can be used.

[0102] The oligosaccharide and / or polysaccharide according to the present invention is present in the composition according to the present invention at a concentration between 0.01% and 20% by weight, preferably between 0.05% and 10% by weight, and even better between 0.05% and 5% by weight, based on the total weight of the composition (i.e., the total concentration of the oligosaccharide and / or polysaccharide in the composition according to the present invention).

[0103] Mixture of oligosaccharides and / or polysaccharides

[0104] According to a preferred embodiment of the present invention, in the composition according to the present invention, the oligosaccharide and / or polysaccharide is used in the form of a mixture.

[0105] In the first embodiment of the present invention, it can be a mixture of the same type of oligosaccharide. For example, as mentioned above, a mixture of FOS can be used, especially a mixture of GF2 + GF3 + GF4, such as Quantum FOS95 from Quantum hi-Tech or , the latter corresponding to a mixture of 37% GF2, 53% GF3, and 10% GF4.

[0106] According to a second embodiment of the invention, it may be a mixture of different types of oligosaccharides and / or polysaccharides. The invention relates in particular to the use of a mixture of inulin with GOS, FOS, soy derived oligosaccharides, pyrodextrins, isomaltooligosaccharides, xylo-oligosaccharides and / or trans-galacto-oligosaccharides. According to a particular embodiment, a mixture of inulin and GOS is used, for example Bioline from the company Gova Ingredients. The invention also relates to the use of a mixture of GOS with FOS.

[0107] Preferably, in this second mode, the composition according to the invention comprises a mixture of oligosaccharides comprising:

[0108] - at least one glucosamine (GOS), and

[0109] - at least one fructooligosaccharide (FOS),

[0110] wherein said oligoglucose (GOS) is present in the composition according to the invention in a concentration of between 0.01% and 10% by weight, preferably between 0.05% and 5% by weight, even better still between 0.1% and 1% by weight, even more preferably between 0.2% and 0.8% by weight relative to the total weight of the composition;

[0111] Wherein the fructooligosaccharides (FOS) are present in the composition according to the invention in a concentration between 0.001% and 5% by weight, preferably between 0.01% and 1% by weight, even better still between 0.01% and 0.5% by weight, even more preferably between 0.05% and 0.3% by weight relative to the total weight of the composition.

[0112] Advantageously, in a second embodiment of the invention, the composition according to the invention comprises a mixture of oligosaccharides comprising:

[0113] - at least one glucosamine (GOS), and

[0114] - at least one fructooligosaccharide (FOS),

[0115] Therein the [GOS / FOS] mass ratio is at least 2, preferably between 2 and 4, even better still between 3 and 4.

[0116] Particularly useful is a mixture of prebiotic oligosaccharides and probiotic microorganisms, which is marketed by the company Solabia as Sold under the name.

[0117] The mixture comprises in particular:

[0118] - between 60% and 80% by weight of at least one glucosamine (GOS), relative to the total weight of the mixture, and

[0119] - between 10 and 25% by weight of at least one fructo-oligosaccharide (FOS), relative to the total weight of the mixture.

[0120] In particular, the mixture comprises 70% by weight of oligosaccharides (GOS), 19% by weight of Juglans radiata tuber juice, 1% by weight of Lactobacillus acidophilus and Lactobacillus casei, and 10% by weight of maltodextrin.

[0121] Probiotics

[0122] Independently of the two variants described above, the composition according to the invention may also comprise at least one probiotic microorganism.

[0123] For the purposes of the present invention, the term "probiotic microorganisms" is intended to mean live microorganisms which, when consumed in adequate amounts, have a positive effect on the health of their host ("Joint FAO / WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotic in Food Including Powder Milk with Live Lactic Acid Bacteria", October 6, 2001), and which may in particular improve the intestinal microbial balance.

[0124] In the case of the skin, the probiotic microorganisms are those which, when applied to the skin in suitable amounts, have a positive effect on the aesthetics of the skin and mucous membranes.

[0125] More particularly, they are probiotic microorganisms from the group of lactic acid bacteria, such as, in particular, the genus Lactobacillus. By way of example of these lactic acid bacteria, mention may in particular be made of Lactobacillus casei, Lactobacillus acidophilus and mixtures thereof.

[0126] Specific examples of probiotic microorganisms are Lactobacillus acidophilus, Lactobacillus pepticus, Lactobacillus curvature, Lactobacillus delbrueckii lactis, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus casei, Lactobacillus rhamnosus (GG Lactobacillus), Lactobacillus sakei, Lactococcus lactis, Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus salivarius, Lactobacillus curvature, Lactobacillus plantarum, Lactobacillus sakei, Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus fermentum, Lactobacillus reuteri, Lactobacillus bulgaricus, Lactobacillus longus, Lactobacillus lactis, Bifidobacterium longum, and mixtures thereof.

[0127] Generally, a composition according to the invention generally comprises from 0.0001 to 20% by weight of at least one probiotic microorganism relative to the total weight of the composition.

[0128] Advantageously, the probiotic microorganisms are present in the composition according to the invention in a concentration of between 0.0001% and 10% by weight, preferably between 0.001% and 5% by weight, even more preferably between 0.001% and 1% by weight relative to the total weight of the composition.

[0129] The microorganisms may be contained in the composition according to the invention in live, semi-live or inactivated, dead form.

[0130] They may also be present in the form of fractions of cellular components or in the form of metabolites.The microorganisms, metabolites or fractions may be introduced in the form of lyophilized powders, culture supernatants and / or, where appropriate, in concentrated form.

[0131] According to a preferred embodiment of the invention, the microorganisms are in an inactivated form, in particular inactivated by heat or high pressure.

[0132] The terms "in inactivated form", "in non-regenerable form" and "in dead form" are used synonymously herein.

[0133] Bacteria "in semi-active form" are bacteria which have partially or completely lost their proliferative properties.

[0134] In case the microorganisms are formulated in the composition in live form, the amount of live microorganisms may range from 10 to 10 g of the composition. 3 cfu / g to 10 15 cfu / g, especially from 10 5 cfu / g to 10 15 cfu / g and more particularly from 10 7 cfu / g to 10 12 cfu / g of microorganisms.

[0135] In a particularly preferred embodiment, the composition according to the invention comprises a mixture of oligosaccharides comprising:

[0136] - at least one glucosamine (GOS), and

[0137] - at least one fructooligosaccharide (FOS),

[0138] wherein said oligoglucose (GOS) is present in the composition according to the invention in a concentration of between 0.01% and 10% by weight, preferably between 0.05% and 5% by weight, even better still between 0.1% and 1% by weight, even more preferably between 0.2% and 0.8% by weight relative to the total weight of the composition;

[0139] wherein said fructooligosaccharides (FOS) are present in the composition according to the invention in a concentration of between 0.001% and 5% by weight, preferably between 0.01% and 1% by weight, even better still between 0.01% and 0.5% by weight, even more preferably between 0.05% and 0.3% by weight relative to the total weight of said composition;

[0140] And moreover at least one probiotic microorganism chosen from the bacteria of the genus Lactobacillus, in particular Lactobacillus casei, Lactobacillus acidophilus and mixtures thereof, wherein said probiotic microorganism represents between 0.0001% and 10% by weight, preferably between 0.001% and 5% by weight, even better still between 0.001% and 1% by weight relative to the total weight of the composition.

[0141] Advantageously, the composition according to the invention comprises a mixture of oligosaccharides comprising:

[0142] - at least one glucosamine (GOS), and

[0143] - at least one fructooligosaccharide (FOS),

[0144] Therein the [GOS / FOS] mass ratio is at least 2, preferably between 2 and 4, even better still between 3 and 4.

[0145] Particularly useful is a mixture of prebiotic oligosaccharides and probiotic microorganisms, which is marketed by the company Solabia as Sold under the name.

[0146] The mixture comprises in particular:

[0147] - between 60% and 80% by weight of at least one glucosamine (GOS), relative to the total weight of the mixture, and

[0148] - between 10% and 25% by weight of at least one fructo-oligosaccharide (FOS), relative to the total weight of the mixture, and

[0149] - between 0.001% and 15% by weight of at least one probiotic microorganism, relative to the total weight of the mixture, in particular chosen from Lactobacillus casei, Lactobacillus acidophilus and mixtures thereof.

[0150] In particular, the mixture comprises 70% by weight of oligosaccharides (GOS), 19% by weight of Juglans radiata tuber juice, 1% by weight of Lactobacillus acidophilus and Lactobacillus casei, and 10% by weight of maltodextrin.

[0151] Mannose

[0152] The composition according to the invention comprises mannose monosaccharide.

[0153] Mannose is a monosaccharide (simple non-hydrolyzable sugar) consisting of 6 carbons; it is a hexose. Its empirical formula is C6H 12 O6, like glucose, is the C2 diastereomer of glucose (that is, its spatial configuration is exactly the same, except for the substituent on carbon 2, where it is opposite to that of glucose).

[0154] The monosaccharide in question according to the present invention corresponds to the following formula (IV).

[0155] [Chemical formula 4]

[0156]

[0157] Of course, all enantiomeric forms of mannose are contemplated according to the present invention.

[0158] Mannose can also be found in solvated forms (including hydrates) and as a mixture of D and L stereoisomers: DL-mannose.

[0159] According to a preferred embodiment, the mannose monosaccharide is D-mannose of the following formula (V).

[0160] [Chemical formula 5]

[0161]

[0162] D-Mannose occurs naturally in plants, especially certain fruits (including cranberries), or in hardwoods (beech and birch).

[0163] As an illustration of D-mannose suitable for the present invention, mention may be made in particular of the D-mannose produced by Danisco Company or D-Mannose sold by the company.

[0164] In general, mannose monosaccharide may be used from 0.01% to 20% by weight, preferably from 0.05% to 10% by weight and most preferably from 0.1% to 5% by weight relative to the total weight of the composition containing it.

[0165] As mentioned previously, the composition according to the present invention comprises a physiologically acceptable medium. More specifically, the physiologically acceptable medium may include water and / or one or more water-soluble organic solvents, which may be selected from linear or branched C1-C6 monohydric alcohols, such as ethanol, isopropanol, tert-butyl alcohol or n-butyl alcohol; polyols, such as glycerol, propylene glycol, hexylene glycol (or 2-methyl-2,4-pentanediol) and polyethylene glycol; polyol ethers, such as dipropylene glycol monomethyl ether; and mixtures thereof.

[0166] Preferably, the water content of the composition according to the invention ranges from 20% to 95% by weight and better still from 40% to 90% by weight relative to the total weight of the composition.

[0167] Advantageously, the composition comprises one or more water-soluble organic solvents in a content ranging from 0.5% to 25% by weight, preferably from 5% to 20% by weight and even better still from 10% to 15% by weight relative to the total weight of the composition.

[0168] Presentation

[0169] The cosmetic composition according to the invention is applied topically.

[0170] The composition for external topical application can be an aqueous, hydroalcoholic or oily solution, a solution or dispersion of the lotion or serum type, an emulsion of liquid or semi-liquid consistency of the emulsion type (obtained by dispersing a fatty phase in an aqueous phase (O / W) or vice versa (W / O)), or a suspension or emulsion of soft, semi-solid or solid consistency of the cream type, aqueous or anhydrous gel, microemulsion, microcapsule, microparticles, or vesicular dispersion of the ionic and / or non-ionic type.

[0171] These compositions were prepared according to the general methods.

[0172] The topical composition according to the invention can advantageously be formulated in any form suitable for skin care, in particular in the form of a cream for cleansing, protecting, treating or caring for the face or body, a mask for application to the skin or hair, a make-up remover, a body protection or care milk, a lotion, a gel or a foam for skin care (e.g. a cleansing lotion, a shower composition).

[0173] Alternatively, the topical composition according to the invention can advantageously be formulated in any form of presentation suitable for hair care, in particular in the form of a hair lotion, a shampoo (in particular an antidandruff agent), a conditioner, a detangling agent, a hair cream or gel, a therapeutic lotion, a lotion or gel to prevent hair loss, an antiparasitic shampoo, a therapeutic shampoo (in particular an anti-sebum shampoo), a head care agent (in particular an anti-irritant, anti-aging, restructuring product).

[0174] Auxiliary agents

[0175] In a known manner, the presentation forms intended for topical application may also include adjuvants common in the cosmetic, pharmaceutical and / or dermatological fields, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, masking agents, fatty substances (such as oils), emulsifiers, odor absorbers and colorants. The amounts of these various adjuvants are those commonly used in the field under consideration, for example from 0.01% to 20% of the total weight of the composition. Depending on their nature, these adjuvants can be introduced into the fatty phase and / or into the aqueous phase.

[0176] As hydrophilic gelling agents that may be used in the composition according to the invention, mention may be made of modified or unmodified carboxyvinyl polymers, such as the products sold under the names Carbopol (INCI name: CARBOMER) and Pemulen (INCI name: ACRYLATES / C10-30 ALKYL ACRYLATE CROSSPOLYMER) by Goodrich, or crosslinked sodium polyacrylate (INCI name: SODIUM POLYACRYLATE), such as the product sold under the name Cosmedia SP by Cognis; polyacrylamide; 2-acrylamido-2-methylpropanesulfonic acid homopolymers, such as the product sold under the name Hostacerin AMPS by Clariant (INCI name: AMMONIUM POLYPROPYLENEDIMETHYLTAURATE); anionic copolymers of crosslinked acrylamide and AMPS, which are provided in the form of emulsions, such as the products sold under the names Sepigel 305 (CTFA name: Polyacrylamide / C13-14 Isoparaffin / Laureth-7) by SEPPIC and under the name Simulgel 506 (CTFA name: Polyacrylamide / C13-14 Isoparaffin / Laureth-7) by SEPPIC. 600 (CTFA name: acrylamide / sodium acryloyldimethyltaurate copolymer / isohexadecane / polysorbate 80); acrylate / acrylonitrile copolymers, such as Hypan SS201 sold by Kingston; synthetic neutral polymers, such as poly-N-vinylpyrrolidone; polysaccharides, such as guar gum, xanthan gum and cellulose-based derivatives. The amount of these polymers can range, for example, from 0.05% to 5% by weight and better still from 0.1% to 3% by weight relative to the total weight of the composition.

[0177] Lipophilic gelling agents that may be mentioned include modified clays such as bentonite, metal salts of fatty acids such as aluminum stearate, and hydrophobic silica, or alternatively ethylcellulose and polyethylene.

[0178] When the composition of the invention is an emulsion, the proportion of the fatty phase can range from 5% to 80% by weight, and preferably from 10% to 50% by weight, relative to the total weight of the composition. The oils, emulsifiers and co-emulsifiers used in the composition in the form of an emulsion are selected from those conventionally used in cosmetics and / or dermatology. Emulsifiers and co-emulsifiers may be present in the composition in a proportion ranging from 0.3% to 30% by weight, and preferably from 0.5% to 20% by weight, relative to the total weight of the composition.

[0179] When the composition of the invention is an oily solution or gel, the fatty phase may represent more than 90% by weight of the total composition.

[0180] As oils that can be used, examples that may be mentioned include:

[0181] - hydrocarbon-based oils of vegetable origin, such as liquid fatty acid triglycerides containing from 4 to 10 carbon atoms, for example heptanoic or caprylic triglycerides, or alternatively, for example, sunflower oil, corn oil, soybean oil, marrow oil, grapeseed oil, sesame seed oil, hazelnut oil, almond oil, macadamia oil, arara oil, castor oil, avocado oil, caprylic / capric triglycerides, for example those sold by Stearineries Dubois or those sold under the names Miglyol 810, 812 and 818 by Dynamit Nobel, jojoba oil and shea butter;

[0182] - synthetic esters and ethers, especially of fatty acids, for example oils of the formula R1COOR2 and R1OR2, in which R1 represents a fatty acid residue containing from 8 to 29 carbon atoms and R2 represents a branched or unbranched hydrocarbon chain containing from 3 to 30 carbon atoms, for example purcellin oil, isononyl isononanoate, isopropyl myristate, 2-ethylhexyl palmitate, 2-octyldodecyl stearate, 2-octyldodecyl erucate or isostearyl isostearate; hydroxylated esters, for example isostearyl lactate, octylhydroxystearate, octyldodecylhydroxystearate, diisostearyl malate, triisocetyl citrate and fatty alcohol heptanoates, caprylates and caprates; polyol esters, for example propylene glycol dicaprylate, neopentyl glycol diheptanoate and diethylene glycol diisononanoate; and pentaerythritol esters, for example pentaerythritol tetraisostearate;

[0183] - linear or branched hydrocarbons of mineral or synthetic origin, such as liquid paraffin (which may be volatile or non-volatile) and its derivatives, petrolatum, polydecene, hydrogenated polyisobutene (for example palm oil), fatty alcohols having from 8 to 26 carbon atoms (for example cetyl alcohol, stearyl alcohol and mixtures thereof (cetostearyl alcohol)), octyldodecanol, 2-butyloctanol, 10 2-hexyldecanol, 2-undecylpentadecanol, oleyl alcohol or linolenyl alcohol; silicone oils such as volatile or nonvolatile polymethylsiloxanes (PDMS) with a linear or cyclic silicone chain, which are liquid or pasty at ambient temperature, in particular cyclopolydimethylsiloxanes (cyclomethicones) such as cyclohexasiloxane; polydimethylsiloxanes containing alkyl, alkoxy or phenyl groups at the side chains or at the ends of the silicone chain, said groups having from 2 to 24 carbon atoms; phenylated silicones, for example phenyl trimethicone, phenyl dimethicone, phenyl trimethylsiloxydiphenylsiloxane, diphenyl dimethicone, diphenylmethyl diphenyl trisiloxane, 2-phenylethyl trimethylsiloxysilicate, and polymethylphenylsiloxane; and

[0184] - mixtures thereof.

[0185] In the list of oils mentioned above, the term "hydrocarbon-based oil" means any oil comprising mainly carbon and hydrogen atoms and possibly ester, ether, fluorine, carboxylic acid and / or alcohol groups.

[0186] As emulsifiers, mention may be made of amphoteric, cationic, anionic or nonionic surfactants, used alone or in mixtures, and optionally coemulsifiers.

[0187] For O / W emulsions, mention may be made, for example, of nonionic emulsifiers as emulsifiers, such as oxyalkylenated (more particularly polyoxyethylenated) fatty acid esters of glycerol; oxyalkylenated fatty acid esters of sorbitan; oxyalkylenated (oxyethylenated and / or oxypropylenated) fatty acid esters; oxyalkylenated (oxyethylenated and / or oxypropylenated) fatty alcohol ethers; sugar esters, in particular oxyalkylenated sugar esters, esters of phosphoric acid and fatty alcohols; and mixtures thereof.

[0188] In a preferred embodiment, the composition according to the invention also comprises at least one ingredient chosen from: silicone fatty substances, such as silicone oils, gums and waxes; non-silicone fatty substances, such as oils, pastes and waxes of vegetable, mineral, animal and / or synthetic origin; fatty acids having from 8 to 32 carbon atoms; synthetic esters and ethers, in particular of the formula R 1 COOR 2 and R 1 OR 2 Synthetic esters and ethers, wherein R 1 represents a fatty acid residue containing from 8 to 29 carbon atoms, and R 2It represents a branched or unbranched hydrocarbon chain containing from 3 to 30 carbon atoms; a linear or branched hydrocarbon of mineral or synthetic origin; a fatty alcohol having from 8 to 26 carbon atoms; water; a C2-C6 monohydric alcohol; a diol selected from propylene glycol, butylene glycol, pentanediol; a ketone; a thickener, an emulsifier, a surfactant, a gelling agent, a fragrance, a filler, a colorant, a humectant, a vitamin selected from vitamin A, E, B3, a polymer.

[0189] The amounts of these various ingredients are those usually used in the field under consideration, for example from 0.01% to 20% of the total weight of the composition.

[0190] Cosmetic treatments and cosmetic uses

[0191] The invention also relates to a method for cosmetic care treatment of the skin and / or mucous membranes, which method comprises applying a composition according to the invention to said skin and / or mucous membranes, in particular which has been subjected to an external aggression.

[0192] In particular, the cosmetic treatment method according to the invention makes it possible to maintain and / or restore the balance of the bacterial flora of the skin and / or mucous membranes.

[0193] Furthermore, the cosmetic treatment method according to the invention makes it possible to prevent and / or treat impairments of the aesthetic qualities of the skin and / or mucous membranes, in particular to prevent and / or treat dry and / or rough skin.

[0194] The cosmetic treatment method according to the invention also makes it possible to prevent and / or treat sensitive skin, in particular to prevent and / or treat discomforts of sensitive skin.

[0195] The invention also relates to the topical cosmetic use of said combination as defined above and optionally at least one probiotic microorganism as defined above for caring for the skin, in particular for skin that has been subjected to external aggressions.

[0196] The invention also relates to the use of said combination for maintaining and / or restoring the balance of the bacterial flora of the skin and / or mucous membranes.

[0197] Furthermore, the present invention relates to the use of said combination for preventing and / or treating sensitive skin, in particular for preventing and / or treating discomfort of sensitive skin.

[0198] As for discomfort sensations, these may typically be itching, warmth or stinging sensations and / or pulling sensations. Representative visible skin signs that may especially be mentioned include itching, dry patches, erythema and / or redness.

[0199] These findings are more common on the most exposed areas of the body (ie, hands, feet, face, and scalp).

[0200] Therefore, the appearance of signs of discomfort that appear within a few minutes of the individual's contact with the triggering factor is one of the basic characteristics of sensitive skin. Those of main concern are dysaesthesic sensations, which are signs of sensory nerves. Dysaesthesic sensations are understood as sensations such as tingling, tingling, itching (itching or pruritus), warmth, discomfort, pulling, etc. These subjective signs are usually present in the absence of visible clinical signs such as desquamation. It is now known that these skin intolerance reactions are related in particular to the release of neuropeptides by nerve endings in the epidermis and dermis.

[0201] The invention further relates to the prevention and / or treatment of manifestations of irritation discomfort in sensitive skin.

[0202] The present invention also proposes to prevent and / or reduce skin discomfort, such as, for example, stinging, tingling, itching, warmth, discomfort, erythema and / or pulling, particularly in people with sensitive skin.

[0203] In contrast to skin that is considered "allergic," the reactivity of sensitive skin is not an immune process, ie, it is not produced in response to the presence of an allergen.

[0204] Furthermore, its response mechanism is said to be "non-specific." In this respect, it differs from skin that exhibits inflammatory and allergic reactions such as dermatoses, eczema and / or ichthyosis.

[0205] For the purposes of the present invention, the term "sensitive skin" encompasses irritated skin and intolerant skin.

[0206] In the context of the present invention, the sensitive skin under consideration does not show any inflammatory properties.

[0207] Finally, the invention relates to the use of said combination for preventing and / or treating impairments of the aesthetic qualities of the skin and / or mucous membranes, in particular for preventing and / or treating dry and / or rough skin.

[0208] Dry skin manifests itself primarily as a sensation of discomfort, such as pulling and / or tightness. This dry skin may also feel rough to the touch and / or appear to be covered with scales. When the skin is slightly dry, the scales are numerous but barely visible to the naked eye. As the condition worsens, they become fewer and fewer in number but increasingly visible to the naked eye.

[0209] The causes of this dry skin may be of constitutional or acquired type.

[0210] The combination is preferably intended for the treatment of non-pathological constitutional dry skin or non-pathological acquired dry skin.

[0211] In the case of acquired dry skin, the involvement of external parameters such as exposure to chemical agents, adverse climatic conditions or the sun, or certain therapeutic treatments (eg with retinoids) is a determining factor. Under these external influences, the skin may then dry out temporarily and locally.

[0212] Nonpathological constitutional dry skin is dry skin whose severity may depend on external factors as already indicated. This skin category includes senile skin (characterized by a general decrease in skin metabolism with age), fragile skin (very sensitive to external factors and often accompanied by erythema) and common xerosis (which may be of hereditary origin and which manifests itself mainly on the face, limbs and backs of the hands).

[0213] The following examples illustrate the invention and are given by way of non-limiting illustration only.

[0214] Unless otherwise mentioned, in the following the percentages are given on a weight basis.

[0215] The expression "at least one" is equivalent to "one or more".

[0216] Unless otherwise indicated, the expressions “between . . . and . . ” and “ranging from . . . to . . ”, “at least . . . ” or “at most . . . ” should be understood to include the limits.

[0217] The following examples and drawings are provided by way of illustration and do not limit the scope of the present invention.

[0218] Examples

[0219] Example 1 - Evaluation of the recovery of skin bacterial flora after application of a composition according to the invention following use of an aggressive cleanser.

[0220] A group of 30 women between the ages of 30 and 50 were selected. The facial skin of the study subjects was cleansed using an aggressive cleanser.

[0221] The ingredients of the cleaning composition include the following: water, triethanolamine, glycerin, myristic acid, lauric acid, cocamidopropyl betaine, lauryl phosphate, hydroxypropyl methylcellulose, pentasodium triphosphate, O-cymene-5-ol (isopropyl methylphenol), benzophenone-4, and butylated hydroxytoluene.

[0222] Assess the skin on the subject's cheek:

[0223] - On bare skin (D0): at T0be, T0af, T3h and T6h;

[0224] - immediately after application of composition 1 (D5): at T3h and T6h;

[0225] - 14 days after treatment with composition 1 (D19): at T3h and T6h.

[0226] T0 be: before cleaning;

[0227] T0 af: immediately after cleaning;

[0228] T3h: 3 hours after cleaning;

[0229] T6h: 6 hours after cleaning.

[0230] The assessment was conducted based on the following criteria:

[0231] Roughness:

[0232] - Roughness assessment by clinical scoring: the clinician assesses the tactile roughness of the skin without touching the cheek area where the sample has been taken;

[0233] -Levels from 0 to 4 are as follows:

[0234] 0 = Not present: Surface is completely smooth and soft

[0235] 1 = Slight: Slight irregularities and roughness when touched tangentially

[0236] 2 = Moderate: Obvious skin irregularities, rough appearance, and slight induration detected by vertical touch

[0237] 3 = Severe: More noticeable irregularities and roughness, plus skin induration

[0238] 4 = Extreme: Very noticeable irregularities and severe disturbance of skin markings with more obvious signs.

[0239] Dryness:

[0240] - Assessment of dryness by clinical scoring: Clinicians studied the dryness of the facial skin.

[0241] -Levels from 0 to 3 are as follows:

[0242] 0 = No dry skin

[0243] 1 = Slightly dry (slightly rough)

[0244] 2 = Moderate dryness (moderate roughness, some scaling)

[0245] 3 = Severe dryness (noticeable roughness and noticeable scaling).

[0246] Discomfort:

[0247] - Evaluation of discomfort by self-assessment (stinging, itching, warmth, pulling): the interrogator asked the volunteers to perform a self-assessment during the dynamics period immediately after meticulous cleaning and after 3h and 6h, using the following scale from 0 to 4:

[0248] 0 = Not at all

[0249] 1 = Mild

[0250] 2 = Medium

[0251] 3 = Severe

[0252] 4 = Very serious.

[0253] Bacterial load and bacterial community analysis:

[0254] - Assessment of total bacterial load by QPCR (QPCR on V1-V3 16S rRNA in triplicate (primer set 27F / 534R) KAPA Biosystems kit with SYBER Green n)

[0255] - Metagenomic analysis of the bacterial community by means of next-generation sequencing (amplification of V1-V3 on the Illumina MiSeq platform (primer set 27F / 534R)) and subsequent taxonomic assignment using SILVA bases and analysis of OTUs by QIIME (open reference), enabling calculation of alpha diversity (Shannon index) and beta diversity (UniFrac distance):

[0256] The following composition 1 according to the invention was prepared:

[0257] [Table 1]

[0258]

[0259]

[0260] *D-Mannose sold by Danisco.

[0261] **Sold by Solabia , which contains 70% by weight of oligosaccharides (GOS), 19% by weight of Juvenile desmodiolus tuber juice, 1% by weight of Lactobacillus acidophilus and Lactobacillus casei, and 10% by weight of maltodextrin.

[0262] Composition 1 is prepared as follows: the components of the aqueous phase are homogenized under stirring and the whole is heated to a temperature of 60° C. The components of the fat phase are added to this heated mixture, which components have previously been premixed and heated to a temperature of 60° C. The gelling agent is then added. The whole is kept under stirring until emulsified and the mixture is cooled to a temperature of 22° C. to 23° C. under continued stirring.

[0263] Apply Composition 1 to the entire face.

[0264] Assessment of skin roughness

[0265] [Table 2]

[0266]

[0267] The values ​​listed in Table 2 above represent the mean values ​​(n=30) of the skin roughness scores (assessed according to the scale detailed above).

[0268] in conclusion : Using the area under the curve, after fourteen days of treatment (at D19), the application of composition 1 according to the invention showed a significant reduction in skin roughness (p<0.001) relative to no application of the composition.

[0269] Assessment of skin dryness

[0270] [Table 3]

[0271]

[0272] The values ​​listed in Table 3 above represent the mean values ​​(n=30) of the skin dryness scores (assessed according to the scale detailed above).

[0273] in conclusion :

[0274] - using the area under the curve, after fourteen days of treatment (at D19), the application of composition 1 according to the invention showed a significant reduction in skin dryness relative to no application of composition (p=0.0001),

[0275] - After fourteen days of treatment (at D19), the application of composition 1 according to the invention showed a significant reduction in skin dryness relative to a single application of the composition at D5 (p<0.0001).

[0276] Assessment of discomfort

[0277] [Table 4]

[0278]

[0279] The values ​​reported in Table 4 above represent the mean values ​​(n=30) of the skin discomfort scores (assessed according to the scale detailed above).

[0280] in conclusion :

[0281] - Using the area under the curve, the application of composition 1 according to the invention showed a significant reduction in skin discomfort after a single application on D5 relative to no application of the composition (p<0.001).

[0282] Furthermore, after fourteen days of treatment (at D19), the application of composition 1 according to the invention showed a significant reduction in skin discomfort relative to a single application of the composition at D5 (p<0.001).

[0283] - Finally, after fourteen days of treatment (at D19), the application of composition 1 according to the invention showed a significant reduction in the perception of skin discomfort relative to no application of composition (p<0.001).

[0284] Evaluation of the base 10 logarithmic bacterial load

[0285] [Table 5]

[0286]

[0287] The values ​​reported in Table 5 above represent the mean values ​​(n=30) of bacterial loads using the evaluation method detailed above.

[0288] in conclusion :

[0289] - Using the area under the curve, application of composition 1 according to the invention showed a significant increase in the bacterial load after a single application at D5 relative to no application of the composition (p<0.001).

[0290] - Furthermore, after fourteen days (at D19), the application of composition 1 according to the invention showed a significant increase in the bacterial load (p<0.001) relative to no application of the composition, indicating a complete recovery of the bacterial load.

[0291] Alpha diversity (Shannon index) assessment

[0292] [Table 6]

[0293]

[0294] The values ​​reported in Table 6 above represent the median alpha diversity using the evaluation method detailed above.

[0295] in conclusion

[0296] - A significant decrease in alpha diversity was found after cleaning (p<0.05). No recovery was found on bare skin, with a significant decrease in diversity still present at 3h and 6h after cleaning (p<0.05).

[0297] - Starting from the initial application of composition 1 according to the invention at D5, a recovery of alpha diversity was observed (p=0.024), predicting the start of recovery.

[0298] - Fourteen days later (at D19), application of composition 1 according to the invention showed a significant increase in alpha diversity, indicating restoration of alpha diversity, with the diversity at T6h after cleaning not being different from that at T0 before cleaning (p>0.05), an indicator of complete restoration of alpha diversity.

[0299] - Furthermore, after fourteen days (at D19), the application of composition 1 according to the invention showed a significantly different restoration of alpha diversity relative to no application of this composition (p=0.023).

[0300] β-diversity / UniFrac distance assessment-R value represents the phylogenetic distance relative to T0 before cleaning.

[0301] [Table 7]

[0302]

[0303] The values ​​reported in Table 7 above represent the mean values ​​of beta diversity using the evaluation method detailed above (n=30). The greater the reduction in R value, the smaller the phylogenetic distance relative to T0 before cleaning and the greater the resilience of the bacterial community.

[0304] in conclusion :

[0305] - Cleansing with aggressive detergents significantly altered the structure of the skin bacterial community. It is important to note that the changes after T3h were greater than at T0 after cleaning, meaning that skin bacteria were less likely to recover.

[0306] - Lower R values ​​are already observed immediately after a single application of composition 1 according to the invention relative to the untreated, which means the onset of recovery.

[0307] - Fourteen days of application of composition 1 according to the invention resulted in smaller changes immediately after cleaning and also in R values ​​that were still lower relative to a single application: 3 h after cleaning, the R values ​​were very low and the p values ​​were no longer significantly different, indicating that the bacterial community was similar to that before cleaning and indicating a complete recovery of the bacterial β diversity.

[0308] Example 2 - Comparison of the recovery of skin bacterial flora after application between a composition according to the invention and a composition not according to the invention following use of an aggressive cleanser.

[0309] A group of 30 women aged between 55 and 65 years were selected. The facial skin of the subjects in the study was cleansed using an aggressive cleanser. The protocol applied was the same as that set out in Example 1 above, but composition 1 was replaced by compositions 2 (according to the invention) and 3 (not according to the invention).

[0310] The ingredients of the cleaning composition include the following: water, triethanolamine, glycerin, myristic acid, lauric acid, cocamidopropyl betaine, lauryl phosphate, hydroxypropyl methylcellulose, pentasodium triphosphate, O-cymene-5-ol (isopropyl methylphenol), benzophenone-4, and butylated hydroxytoluene.

[0311] Assess the skin on the subject's cheek:

[0312] - On bare skin (D0): at T0be, T0af, T3h and T6h;

[0313] - immediately after application of the composition (D3): at T3h and T6h;

[0314] - 15 days after treatment with the composition (D18): at T3h and T6h.

[0315] T0 be: before cleaning;

[0316] T0 af: immediately after cleaning;

[0317] T3h: 3 hours after cleaning;

[0318] T6h: 6 hours after cleaning.

[0319] The assessment was conducted based on the following criteria:

[0320] - Pulling sensation

[0321] - Bacterial load

[0322] - Alpha diversity (Shannon index)

[0323] - Beta diversity

[0324] The scoring scales used were the same as those shown in Example 1.

[0325] The following composition 2 according to the invention and composition 3 not according to the invention were prepared:

[0326] [Table 8]

[0327]

[0328] *D-Mannose sold by Danisco.

[0329] **Sold by Solabia , which contains 70% by weight of oligosaccharides (GOS), 19% by weight of Juvenile desmodiolus tuber juice, 1% by weight of Lactobacillus acidophilus and Lactobacillus casei, and 10% by weight of maltodextrin.

[0330] Compositions 2 and 3 were prepared as follows: The compounds were not cold soluble and were therefore mixed and heated to 80°C to form a pre-emulsion. Compositions 2 and 3 were then cooled to 35°C. The gelling agent was then added and dissolved. The probiotic part was added at ambient temperature.

[0331] Apply compositions 2 and 3 to the entire face.

[0332] Assessment of pulling sensation

[0333] [Table 9]

[0334]

[0335] [Table 10]

[0336]

[0337] Conclusion: From the first application of both compositions 2 and 3, a significantly faster decrease was observed in the pulling caused by aggressive cleaning agents compared to bare skin (D0) (p<0.001). After 15 days of application of both compositions 2 and 3, they provided significant protection against the pulling sensation caused by aggressive cleaning agents compared to bare skin (p<0.001).

[0338] Bacterial load assessment

[0339] [Table 11]

[0340]

[0341]

[0342] [Table 12]

[0343]

[0344] Conclusion: Application of the two compositions 2 and 3 allowed a significantly faster and complete recovery of the bacterial load from 3 h onwards (p<0.0001); this efficacy was also confirmed after 15 days of application, with both formulations allowing a significantly complete and faster recovery of the total bacterial load from 3 h onwards (p<0.0001).

[0345] Alpha diversity (Shannon index) assessment

[0346] [Table 13]

[0347]

[0348] The values ​​reported in Table 13 above (Composition 3) represent the average values ​​of alpha diversity assessed by the method detailed above.

[0349] The recovery observed on D3 was better but not significantly different from D0, and no significant difference in bacterial diversity recovery was observed between D0 (bare skin), D3 (one application) and D18 (14 days application). Therefore, there was no better recovery compared to bare skin.

[0350] [Table 14]

[0351]

[0352] The values ​​reported in Table 14 above (Composition 2 according to the invention) represent the average values ​​of the alpha diversity assessed by the method detailed above.

[0353] Significant recovery was observed at D18 (T6h) relative to D0 (T6h) (p<0.005). After 15 days of use, skin bacterial diversity recovered 2.73 times faster after erosion. Recovery was greater and faster at D18 relative to bare skin at D0 and a single application of Composition 2 at D3.

[0354] Evaluation of β diversity—R values ​​represent phylogenetic distances relative to T0 before cleaning.

[0355] [Table 15]

[0356]

[0357]

[0358] The values ​​reported in Table 15 above (Composition 3) represent the mean values ​​of beta diversity assessed by the method detailed above (n=30). The greater the reduction in R value, the smaller the phylogenetic distance relative to T0 before cleaning and the greater the resilience of the bacterial community.

[0359] Based on the reference data (bare skin) at T0 before cleaning, an increase in R values ​​was found, indicating a change and distance in the bacterial community. At D3 and D18, the R values ​​had decreased, but were still significantly different relative to D0, again showing a change in the bacterial community, and no recovery at D0 and D3 and D18.

[0360] [Table 16]

[0361]

[0362] The values ​​reported in Table 16 above (Composition 2) represent the mean values ​​of beta diversity assessed by the method detailed above (n=30). The greater the reduction in R value, the smaller the phylogenetic distance relative to T0 before cleaning and the greater the resilience of the bacterial community.

[0363] Based on the reference data before washing, no recovery was found at D0: the R value barely changed between after washing (R=0.071) and T6h (R=0.057).

[0364] No recovery was observed at D3; the R value barely changed between immediately after washing (R = 0.097) and T6h (R = 0.079).

[0365] A rapid recovery was observed at D18; the R value dropped rapidly after cleaning (R=0.100) and at T3h (R=0.036) and T6h (R=0.018). A significant total recovery was observed at D18 starting from T3h.

[0366] General conclusions :

[0367] Compositions 2 (according to the invention) and 3 (outside the invention) were able to reduce the pulling sensation.

[0368] Composition 2 according to the invention, which comprises only a combination of oligosaccharides and mannose, is able to restore alpha and beta diversity more quickly and significantly after the use of aggressive cleaning agents.

[0369] References:

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[0371] [2] Nakatsuji T, Gallo RL. Antimicrobial Peptides: Old Molecules with New Ideas. J Invest Dermatol, 2012, 132, 3: 887-895.

[0372] [3] Yuki T, Yoshida H, Akazawa Y et al. Activation of TLR2 Enhances Tight Junction Barrier in Epidermal Keratinocytes. J Immunol 2011, 187, 6, 3230-3237

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Claims

1. A cosmetic composition for topical application, comprising in a physiologically acceptable medium: - at least one oligosaccharide, and - at least one mannose monosaccharide, wherein the oligosaccharides are in the form of a mixture comprising: - at least one glucose oligosaccharide (GOS) comprising α-glucan oligosaccharides, and - at least one fructooligosaccharide (FOS), wherein said oligoglucose (GOS) is present in said composition at a concentration between 0.01% and 10% by weight relative to the total weight of said composition; wherein said fructooligosaccharides (FOS) are present in said composition at a concentration between 0.001% and 5% by weight relative to the total weight of said composition; The composition further comprises at least one probiotic microorganism selected from the group consisting of Lactobacillus acidophilus, Lactobacillus casei and mixtures thereof.

2. The composition according to claim 1, further comprising at least one ingredient chosen from: silicone fatty substances; non-silicone fatty substances; fatty acids having from 8 to 32 carbon atoms; synthetic esters and ethers; linear or branched hydrocarbons of mineral or synthetic origin; fatty alcohols having from 8 to 26 carbon atoms; water; C2-C6 monohydric alcohols; diols chosen from propylene glycol, butylene glycol, pentanediol; ketones; thickeners, emulsifiers, surfactants, gelling agents, fragrances, fillers, colorants, humectants, vitamins chosen from vitamins A, E, B3, polymers.

3. The composition according to claim 2, wherein The silicone fatty substances are selected from silicone oils, gels and waxes; the non-silicone fatty substances are selected from oils, pastes and waxes of plant, mineral, animal and / or synthetic origin; the synthetic esters and ethers are selected from the formula R 1 COOR 2 and R 1 OR 2 Synthetic esters and ethers, wherein R 1 represents a fatty acid residue containing from 8 to 29 carbon atoms, and R 2 represents a branched or unbranched hydrocarbon chain containing from 3 to 30 carbon atoms.

4. The composition according to claim 1, wherein The mannose monosaccharide is present in a concentration of from 0.01% to 20% by weight relative to the total weight of the composition.

5. The composition according to claim 1, wherein The mannose monosaccharide is present in a concentration of from 0.05% to 10% by weight relative to the total weight of the composition.

6. The composition according to claim 1, wherein The mannose monosaccharide is present in a concentration of from 0.1% to 5% by weight relative to the total weight of the composition.

7. The composition according to claim 1, wherein said oligoglucose (GOS) being present in said composition at a concentration between 0.05% and 5% by weight relative to the total weight of said composition; Wherein said fructooligosaccharides (FOS) are present in said composition in a concentration between 0.01% and 1% by weight relative to the total weight of said composition.

8. The composition according to claim 1, wherein The oligoglucose (GOS) is present in the composition at a concentration between 0.1% and 1% relative to the total weight of the composition; Wherein said fructooligosaccharides (FOS) are present in said composition in a concentration between 0.01% and 0.5% by weight relative to the total weight of said composition.

9. The composition according to claim 1, wherein said oligoglucose (GOS) being present in said composition at a concentration between 0.2% and 0.8% by weight relative to the total weight of said composition; Wherein said fructooligosaccharides (FOS) are present in said composition in a concentration between 0.05% and 0.3% by weight relative to the total weight of said composition.

10. The composition according to claim 1, wherein The probiotic microorganisms are present in a concentration of between 0.0001% and 10% by weight relative to the total weight of the composition.

11. The composition according to claim 1, wherein The probiotic microorganisms are present in a concentration of between 0.001% and 5% by weight relative to the total weight of the composition.

12. The composition according to claim 1, wherein The probiotic microorganisms are present in a concentration of between 0.001% and 1% by weight relative to the total weight of the composition.

13. A non-therapeutic cosmetic treatment method for caring for the skin and / or mucous membranes, said method comprising applying to said skin and / or said mucous membranes a composition according to any one of claims 1 to 12.

14. The method of claim 13, wherein the skin and / or the mucous membrane has been subjected to an external insult.

15. The method according to claim 13, for maintaining and / or restoring the balance of the bacterial flora of the skin and / or mucous membranes.

16. Non-therapeutic topical cosmetic use of at least one oligosaccharide according to claim 1 and at least one mannose monosaccharide according to claim 1 and at least one probiotic microorganism according to claim 1 for caring for the skin.

17. The use according to claim 16, wherein the skin is skin that has been subjected to external aggression.

18. Use according to claim 16, for maintaining and / or restoring the balance of the bacterial flora of the skin and / or mucous membranes.

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