A controllable stem cell enricher based on selective cell retention technology

Abstract

The present application provides a controllable stem cell enricher based on selective cell retention technology, including: a base, on which are respectively provided: an enrichment unit, in which a filter is provided, which is used to retain stem cells on the biological enrichment material placed on the filter when the bone marrow flows through; a feeding unit, including a three-way valve channel and a needle tube loaded with bone marrow, and the needle tube is connected to the top and bottom of the enrichment unit respectively through a medical hose; a driving unit, which is used to push and pull the push rod of the needle tube so that the bone marrow can be circulated between the enrichment unit and the needle tube for multiple one-way cycles. The present application uses selective cell retention technology to enrich new transplant materials, and adopts a three-layer filtration structure combined with good enrichment materials internally, so that the filtration is more efficient and the enrichment ratio is higher. At the same time, a controller is used to realize the controllable number of enrichment cycles and enrichment time.

Description

Technical Field

[0001] The present application relates to the technical field of stem cell enrichers, and in particular to a controllable stem cell enricher based on selective cell retention technology. Background Art

[0002] The stem cell enricher meets the current demand for stem cells in the treatment of bone defects and osteoarthritis. It has been used in clinical practice and can solve the pain points of large demand for autologous bone transplantation and bone marrow transplantation. It is now developing in the direction of high efficiency, low cost and high enrichment ratio. In the future, it will be used to treat other organ defects or for the regeneration of other organ tissues.

[0003] In terms of cell enrichment, selective cell retention technology is often used. Selective retention technology is one of the bone marrow enrichment technologies reported by foreign scholars in recent years. It mainly relies on the good adhesion performance of the enrichment material and the appropriate mesh structure. Under the action of negative pressure, the effective osteogenic components in the bone marrow, such as stem cells and osteogenic factors, can be selectively retained in the material when the bone marrow flows through, and then the constructed enriched material is immediately returned to the implant. SCR technology has achieved relatively ideal results in foreign animal experiments and clinical trials.

[0004] In the existing technology, manual enrichment is mainly used in terms of power, and the enrichment technology adopts adsorption filtration method and centrifugal filtration method. For manual cell adsorption filtration enrichment, it consumes manpower and time during surgery or when the number of medical staff is tight. The operator needs to control the enrichment time and number of enrichments in real time, which greatly increases the proportion of stem cell collection time during surgery. For centrifugal filtration, although density centrifugation is mostly used internationally, it has disadvantages such as low recovery efficiency and reduced cell number; the other method uses centrifuge filtration, which has a fast separation speed but a complex structure, and the fixed number of cycles leads to low enrichment efficiency, a long operation time, and the need for related equipment to be used in pairs. Summary of the invention

[0005] In view of the above-mentioned defects of the prior art, the present application provides a controllable stem cell enricher based on selective cell retention technology to solve at least one problem existing in the prior art.

[0006] To achieve the above-mentioned purpose and other related purposes, the present application provides a controllable stem cell enricher based on selective cell retention technology, including: a base, on which are respectively provided: an enrichment unit, in which a filter net is provided, which is used to retain stem cells on the biological enrichment material placed on the filter net when the bone marrow flows through; a feeding unit, including a three-channel one-way valve and a needle tube loaded with bone marrow, and the needle tube is connected to the top and bottom of the enrichment unit respectively through a medical hose; a driving unit, which is used to push and pull the push rod of the needle tube to enable the bone marrow to achieve multiple one-way circulations between the enrichment unit and the needle tube.

[0007] In one embodiment of the present application, under the drive of the driving unit, the bone marrow in the needle tube enters from the top of the enrichment unit, and the bone marrow that infiltrates to the bottom is sucked back into the needle tube from the bottom of the enrichment unit.

[0008] In one embodiment of the present application, the needle tube is installed in a third slot body on the base; wherein, limit slots are provided on both sides of the third slot body, which can be adapted to engage with the push handle of the needle tube to limit the displacement of the barrel of the needle tube.

[0009] In one embodiment of the present application, the three-channel one-way valve is arranged at a position between the enrichment unit and the feeding unit on the base, and includes: a needle tube channel, a third trough body in the same direction for placing the needle tube, for directly inserting the needle connected to the needle tube; a feed channel, whose outlet faces the upper surface of the base and is connected to the top of the enrichment unit by connecting a medical hose; a discharge channel, which is directly connected to the bottom of the enrichment unit or through a medical hose; a one-way valve, arranged at the intersection of the channels, for keeping the feed channel open and the discharge channel closed when the needle tube channel is subjected to a thrust from the needle tube; and for keeping the feed channel closed and the discharge channel open when the needle tube channel is subjected to a suction from the needle tube.

[0010] In one embodiment of the present application, the enrichment unit includes: an enrichment chamber formed by a first trough body on the base; wherein the enrichment chamber is hemispherical or funnel-shaped, and a discharge port is provided at the bottom; a chamber cover, used to cover the enrichment chamber, and a feed port is provided at the top of the chamber cover; a filter screen with a three-layer filter structure is horizontally arranged in the enrichment chamber, on which biological enrichment materials are placed; wherein the gaps in each layer of the filter structure decrease successively from top to bottom.

[0011] In one embodiment of the present application, a clamping portion is provided on the upper plane of the enrichment chamber protruding from the base; wherein, one side of the clamping portion is hinged to one side of the chamber cover through a rotating shaft so that the chamber cover can be flipped and closed relative to the clamping portion; a snap assembly is provided on the other side of the clamping portion and the other side of the chamber cover so as to lock and open the chamber cover and the clamping portion.

[0012] In one embodiment of the present application, the driving unit includes: a motor, which is arranged in the second slot body of the base, and provides forward and reverse rotation force; a transmission mechanism, which is coupled to the motor and the push rod of the needle tube, respectively, and converts the rotational force driven by the motor into a force for axial displacement along the push rod and transmits it to the push rod; a controller, which is arranged on a mounting plate on the base and is electrically connected to the motor, and is used to set the rotation direction, speed and number of times of the motor.

[0013] In one embodiment of the present application, the transmission mechanism includes: a transmission rod, having helical gears at both ends, one end of which is installed on the second groove body to couple the helical gear at the end of the rotating shaft of the motor, and the other end is inserted into the third groove body; a spiral cylinder, arranged in the third groove body, one end of which is provided with a helical gear and coupled to the helical gear inserted into one end of the third groove body of the transmission rod; the other end of which is provided with an internally threaded sleeve; a threaded push rod, arranged in the third groove body, an outer surface of which is provided with an external thread matching the internal thread of the internally threaded sleeve, one end of the threaded push rod is screwed into the internally threaded sleeve, and the other end is provided with a fixing piece for detachably engaging the push rod tail of the needle tube.

[0014] In one embodiment of the present application, the second groove body is provided with a bearing base for fixing the first rolling bearing for the transmission rod to pass through; a second rolling bearing is embedded in one end of the third groove body, into which a fixing rod is inserted, and the fixing rod is used to insert into one end of the spiral cylinder provided with a spiral gear; a bearing fixing seat is provided on the inner side of the third groove body for fixing the third rolling bearing for the internal threaded sleeve to pass through.

[0015] In one embodiment of the present application, the fixing member includes: a fixing portion welded to the other end of the threaded push rod; a slider disposed on both sides of the fixing portion; wherein sliding grooves adapted to the slider are disposed on both sides of the third groove body; and a supporting portion for clamping and supporting the push rod tail of the needle tube.

[0016] In summary, the present application provides a controllable stem cell enricher based on selective cell retention technology, comprising: a base, on which are respectively provided: an enrichment unit, in which a filter is provided, for retaining stem cells on the biological enrichment material placed on the filter when the bone marrow flows through; a feeding unit, comprising a three-channel one-way valve and a needle tube loaded with bone marrow, the needle tube being connected to the top and bottom of the enrichment unit respectively through a medical hose; a driving unit, for pushing and pulling the push rod of the needle tube, so that the bone marrow can be circulated between the enrichment unit and the needle tube in multiple one-way cycles.

[0017] The following beneficial effects were achieved:

[0018] 1) Controllable stem cell enrichment speed and cycle times during surgery. Through the controller, no external equipment is required, which is convenient and fast, reduces the consumption of manpower and time, achieves uniform enrichment of stem cells, and reduces cell loss rate;

[0019] 2) Achieve high stem cell enrichment efficiency and high enrichment ratio. Adopting selective cell retention technology, a three-layer filtration method is adopted from top to bottom, and the pores are from large to small from top to bottom, which greatly improves the enrichment efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 Schematic diagram of the structure of a controllable stem cell enricher based on selective cell retention technology in one embodiment of the present application.

[0021] Figure 2A and 2B They are schematic structural diagrams of a base at different viewing angles in an embodiment of the present application.

[0022] Figure 3 Schematic diagram of the structure of the three-channel one-way valve in one embodiment of the present application.

[0023] Figure 4 Schematic diagram of the structure of a cavity cover in one embodiment of the present application.

[0024] Figure 5 FIG. 4 is a schematic diagram of the structure of a motor in an embodiment of the present application.

[0025] Figure 6 Schematic diagram of the structure of the transmission mechanism in one embodiment of the present application.

[0026] Figure 7 It is a schematic diagram of the fixing member structure in one embodiment of the present application. DETAILED DESCRIPTION

[0027] The following describes the embodiments of the present application through specific examples, and those skilled in the art can easily understand other advantages and effects of the present application from the contents disclosed in this specification. The present application can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present application. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0028] It should be noted that the illustrations provided in the following embodiments are only schematic illustrations of the basic concept of the present application. Although the drawings only show components related to the present application rather than being drawn according to the number, shape and size of components in actual implementation, the type, quantity and proportion of each component in actual implementation may be changed arbitrarily, and the component layout may also be more complicated.

[0029] Throughout the specification, when a part is said to be "connected" to another part, this includes not only the case of "direct connection" but also the case of "indirect connection" by placing other elements therebetween. In addition, when a part is said to "include" a certain constituent element, unless otherwise stated, it does not exclude other constituent elements, but means that other constituent elements may be included.

[0030] The terms first, second and third are used to describe various parts, components, regions, layers and / or segments, but are not limited thereto. These terms are only used to distinguish a certain part, component, region, layer or segment from other parts, components, regions, layers or segments. Therefore, the first part, component, region, layer or segment described below may refer to the second part, component, region, layer or segment within the scope of this application.

[0031] Furthermore, as used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless there is an indication to the contrary in the context. It should be further understood that the terms "comprise", "include" indicate the presence of the described features, operations, elements, components, items, kinds, and / or groups, but do not exclude the presence, occurrence or addition of one or more other features, operations, elements, components, items, kinds, and / or groups. The terms "or" and "and / or" used herein are interpreted as inclusive, or mean any one or any combination. Therefore, "A, B or C" or "A, B and / or C" means "any of the following: A; B; C; A and B; A and C; B and C; A, B and C". Exceptions to this definition will only occur when the combination of elements, functions or operations is inherently mutually exclusive in some way.

[0032] In response to the problems of high cell loss rate and low enrichment efficiency in existing stem cell enrichment, the present application provides a controllable stem cell enricher based on selective cell retention technology. The enricher adopts a three-layer filtration structure combined with good enrichment materials to make the filtration more efficient and the enrichment ratio higher. At the same time, a controller is used to achieve controllable enrichment cycle times and enrichment time.

[0033] like Figure 1 , which shows a schematic diagram of the structure of a controllable stem cell enricher based on selective cell retention technology in an embodiment of the present application. As shown in the figure, the stem cell enricher comprises: a base 1, on which are respectively provided: an enrichment unit 2, a feeding unit 3, and a driving unit 4.

[0034] Among them, the enrichment unit 2 is provided with a filter net, which is used to retain stem cells in the biological enrichment material placed on the filter net when the bone marrow flows through; the feeding unit 3 includes a one-way valve three-channel 310 and a needle tube 320 loaded with bone marrow, and the needle tube 320 is connected to the top and bottom of the enrichment unit 2 respectively through a medical hose 330; the driving unit 4 is used to push and pull the push rod 323 of the needle tube 320.

[0035] Driven by the driving unit 4, the bone marrow in the needle tube 320 enters from the top of the enrichment unit 2, and the bone marrow that seeps to the bottom is sucked back into the needle tube 320 from the bottom of the enrichment unit 2, so that the bone marrow can be circulated between the enrichment unit 2 and the needle tube 320 for multiple one-way cycles.

[0036] In simple terms, the bone marrow loaded in the needle tube 320 is squeezed into the enrichment unit 2 through the driving unit 4. The enrichment unit 2 is provided with a filter screen, and the biological enrichment material (such as biological gel) is placed on the filter screen. The bone marrow is squeezed out from the top of the enrichment unit 2 and flows through the biological enrichment material. The effective osteogenic components in the bone marrow, such as stem cells and osteogenic factors, can be selectively retained on the biological enrichment material, and the bone marrow that permeates to the bottom through the filter screen can be sucked into the needle tube 320 again through the one-way valve pipeline design of the feeding unit 3, and then squeezed into the enrichment unit 2, so that the bone marrow can achieve multiple one-way circulations between the enrichment unit 2 and the needle tube 320. The preset number of times is set by the controller 440, which can greatly improve the enrichment efficiency and reduce the cell loss rate. Finally, the biological enrichment material is taken out and can be used to be implanted back into the body.

[0037] like Figure 2A and 2B As shown, the base 1 in the present application is shown in different perspectives. As shown in the figure, the base 1 specifically includes: a first slot body 110, a second slot body 120, a third slot body 130 and a mounting plate 140.

[0038] Among them, the first trough body 110 mainly constitutes the enrichment chamber of the enrichment unit 2; the second trough body 120 is mainly used to install the motor 410 of the driving unit 4; the third trough body 130 is mainly used to install the needle tube 320 and part of the structure of the driving unit 4; the mounting plate 140 is mainly used to install the controller 440 of the driving unit 4.

[0039] In one embodiment of the present application, the feeding unit 3 mainly includes a one-way valve three-channel 310 and a needle tube 320 loaded with bone marrow.

[0040] Preferably, if Figure 2B As shown, the one-way valve three-channel 310 is arranged between the enrichment unit 2 and the feeding unit 3 on the base 1. Figure 3As shown, the one-way valve three-channel 310 includes:

[0041] The needle tube channel 331 is used for placing the third slot 130 of the needle tube 320 in the same direction, and is used for directly inserting the needle 321 connected to the needle tube 320;

[0042] A feed channel 332, whose outlet faces the upper surface of the base 1 and is connected to the top of the enrichment unit 2 by connecting a medical hose 330;

[0043] A discharge channel 333, which is directly connected to the bottom of the enrichment unit 2 or connected through a medical hose 330;

[0044] The one-way valve 334 is arranged at the intersection of the channels, and is used to keep the feed channel 332 open and the discharge channel 333 closed when the needle tube channel 331 is subjected to the thrust from the needle tube 320; and to keep the feed channel 332 closed and the discharge channel 333 open when the needle tube channel 331 is subjected to the suction from the needle tube 320.

[0045] In simple terms, the push rod 323 of the needle tube 320 can be pushed and pulled according to the driving unit 4. When the push rod 323 is pushed forward, the needle tube channel 331 is connected with the feed channel 332, and the bone marrow in the needle tube 320 can be squeezed into the top of the enrichment unit 2; when the push rod 323 is pulled backward, the needle tube channel 331 is connected with the discharge channel 333, and the bone marrow seeping down from the bottom of the enrichment unit 2 can be sucked into the needle tube 320. Then, by repeatedly pushing and pulling the needle tube 320 several times, the bone marrow can be unidirectionally circulated between the enrichment unit 2 and the needle tube 320 for multiple times, thereby improving the enrichment efficiency and reducing the cell loss rate.

[0046] Preferably, the needle tube 320 is installed on the third slot body 130 on the base 1; wherein, the third slot body 130 is provided with limit slots 131 on both sides, which can be adapted to engage with the push handle 324 of the needle tube 320 to limit the displacement of the needle tube 320.

[0047] Among them, Figure 3As shown, the needle tube 320 is a common medical needle tube 320, including a needle 321, a barrel 322, a push rod 323 inserted into the barrel 332 to perform piston motion, and a push handle 324 extending outward at the tail of the barrel 332. In the present application, when work is required, the needle tube 320 that has sucked in bone marrow can be installed in the third slot 130. Specifically, the needle 321 is inserted into the needle tube channel 331, and then the push handle 324 is aligned and snapped into the limiting slots 131 on both sides of the third slot 130, and finally the tail of the push rod 323 of the needle tube 320 is snapped into the structure (fixing member 450) corresponding to the drive unit 4. When the work is finished, the push rod 323 of the needle tube 320 is separated from the structure of the corresponding drive unit 4, and then separated from the limiting slot 131, and finally pulled out from the needle tube channel 331, and the needle tube 320 can be removed for filling new bone marrow.

[0048] In one embodiment of the present application, the enrichment unit 2 comprises:

[0049] The enrichment chamber 210 formed by the first trough body 110 on the base 1 is as follows Figure 2A As shown; wherein, the enrichment chamber 210 is hemispherical or funnel-shaped, and a discharge port 211 is provided at the bottom; the upper plane of the enrichment chamber 210 protruding from the base 1 is provided with a snap-fit ​​portion 212.

[0050] Cavity cover 220, such as Figure 4 As shown, the chamber cover 220 is used to cover the enrichment chamber 210 , and a feed port 221 is provided on the top of the chamber cover 220 ; a sealing ring 222 is also provided on the inner side of the chamber cover 220 .

[0051] Among them, one side of the locking portion 212 is hinged to one side of the cavity cover 220 through a rotating shaft 230, so that the cavity cover 220 can be flipped and closed relative to the locking portion 212; preferably, a buckle assembly can also be provided on the other side of the locking portion 212 and the other side of the cavity cover 220, so as to lock and open the cavity cover 220 and the locking portion 212.

[0052] In addition, the filter screen of the three-layer filter structure is stacked together and horizontally arranged in the enrichment chamber 210, on which the biological enrichment material is placed; wherein the gaps of each layer of the filter structure decrease from top to bottom. Among them, by borrowing the selective cell retention technology, the enrichment efficiency is greatly improved, and a high enrichment efficiency and high enrichment ratio of stem cells are achieved. It should be noted that this filter screen structure is not shown in the present application, but those skilled in the art should be able to understand it.

[0053] In one embodiment of the present application, the driving unit 4 includes:

[0054] Motor 410, such as Figure 5As shown, the second slot 120 provided on the base 1 provides forward and reverse rotation forces; wherein the motor 410 is further provided with a mounting base 412, and a rotating shaft 411 extends from one end of the motor 410, and a helical gear 460 is provided at the end thereof. The motor 410 is provided with forward and reverse rotation, so that the transmission mechanism can convert the thrust and pull of the push rod 323 corresponding to the needle tube 320.

[0055] The transmission mechanism is coupled to the motor 410 and the push rod 323 of the needle tube 320, respectively, and converts the rotational force driven by the motor 410 into a force for axial displacement of the push rod 323 and transmits it to the push rod 323. In short, the transmission mechanism mainly converts the rotational force of the motor 410 into a push-pull force that can drive the push rod 323 of the needle tube 320 to move forward and backward.

[0056] The controller 440 is disposed on the mounting plate 140 on the base 1 and is electrically connected to the motor 410 for setting the rotation direction, speed and number of times of the motor 410 .

[0057] In addition, if Figure 2A As shown, a three-hole plug is also provided on the outer side of the base 1 , and is connected to the controller 440 and the motor 410 to provide electrical energy to the controller 440 and the motor 410 .

[0058] It should be noted that the driving unit 4 of the present application realizes the controllable stem cell enrichment speed and cycle number. By adopting the controller 440, no external equipment is required for use, which is convenient and fast, solves the consumption of manpower and time, realizes uniform enrichment of stem cells, and reduces the cell loss rate.

[0059] Specifically, the transmission mechanism includes:

[0060] The transmission rod 420 has spiral gears 460 at both ends. Figure 1 As shown, one end of the screw gear 460 is mounted on the second slot 120 to couple (gear engagement) the end of the rotating shaft 411 of the motor 410, and the other end is connected to the second slot 120 to couple (gear engagement) the spiral gear 460 at the end of the rotating shaft 411 of the motor 410. Figure 2B The slot 160 shown in FIG. is inserted into the third slot body 130. Figure 2A As shown, the second tank body 120 is provided with a bearing base 121 for welding and fixing. Figure 1 A first rolling bearing 470 is provided in the middle for the transmission rod 420 to pass through, so that the transmission rod 420 can be relatively fixed by the first rolling bearing 470 without causing the transmission rod 420 to deviate.

[0061] like Figure 6The spiral cylinder 430 shown is arranged in the third slot body 130, and one end of the spiral cylinder 430 is provided with a spiral gear 460 and coupled (gear engagement) with the transmission rod 420 inserted into the spiral gear 460 at one end of the third slot body 130; the other end of the spiral cylinder 431 is provided with an internal thread sleeve. Figure 2A As shown, a second rolling bearing 133 is embedded at one end of the third groove body 130, into which a fixing rod 134 is inserted. The fixing rod 134 is used to insert into one end of the spiral cylinder 430 provided with a spiral gear 460, so that the spiral cylinder 430 is relatively fixed through the second rolling bearing 133, and the spiral cylinder 430 will not be offset.

[0062] The threaded push rod 440 is disposed in the third groove 130, and its outer surface is provided with an external thread that matches the internal thread of the internal thread sleeve 431. One end of the threaded push rod 440 is screwed into the internal thread sleeve 431, and the other end is provided with a fixing member 450 for detachably engaging the tail of the push rod 323 of the needle tube 320. Figure 2A As shown, the inner side of the third groove body 130 is provided with a bearing fixing seat 135 for fixing Figure 1 The third rolling bearing 480 shown in the figure is for the internal threaded sleeve 431 to pass through, so that the threaded push rod 440 can be relatively fixed by the third rolling bearing 480 without causing the threaded push rod 440 to deviate.

[0063] It should be noted that the threads on the helical gears 460 at the end of the rotating shaft 411 at one end of the motor 410, the helical gears 460 at both ends of the transmission rod 420, and the helical gear 460 at one end of the spiral barrel 430 are preferably 45 degrees, so that the two coupled helical gears 460 can be 90 degrees. When the two helical gears 460 are coupled and operated, one rotating helical gear 460 will cause the other coupled helical gear 460 to rotate in coordination at a relatively vertical position, and the other helical gear 460 will not be displaced in the axial direction.

[0064] In the present application, the motor 410 drives the transmission rod 420 which is vertically arranged with the motor 410 to rotate through the spiral gear 460, and the rotation of the transmission rod 420 drives the spiral cylinder 430 which is vertically arranged with the transmission rod 420 to rotate through the spiral gear 460. The threaded cylinder 430 is provided with an internal threaded sleeve 431 at one end facing the needle tube 320, and the threaded push rod 440 is provided with an external thread, which can be adapted to be inserted into the internal threaded sleeve 431. Since the threaded cylinder 430 only rotates and does not displace, the inserted threaded push rod 440 can be axially displaced through rotation under the action of the rotational force, and then the fixing piece 450 provided at the other end of the threaded push rod 440 can relatively engage with the tail of the push rod 323 of the needle tube 320, thereby realizing the axial push-pull action of the push rod 323 of the needle tube 320.

[0065] Furthermore, if Figure 7 As shown, the fixing member 450 includes: a fixing portion 451 welded to the other end of the threaded push rod 440; a slider 452, which is arranged on both sides of the fixing portion 451; wherein the third slot body 130 is provided with a slide groove 132 adapted to the slider 451 on both sides, see Figure 2A The supporting portion 453 is used to engage and support the tail of the push rod 323 of the needle tube 320.

[0066] It should be noted that the tail of the push rod 323 of the needle tube 320 is usually a disc. Therefore, when the threaded push rod 440 rotates, it will not drive the rotation of the tail of the push rod 323 of the needle tube 320, and when the threaded push rod 440 is displaced, it can drive the displacement of the tail of the push rod 323 of the needle tube 320.

[0067] In summary, the present application provides a controllable stem cell enricher based on selective cell retention technology, comprising: a base, on which are respectively provided: an enrichment unit, in which a filter is provided, for retaining stem cells on the biological enrichment material placed on the filter when the bone marrow flows through; a feeding unit, comprising a three-channel one-way valve and a needle tube loaded with bone marrow, the needle tube being connected to the top and bottom of the enrichment unit respectively through a medical hose; a driving unit, for pushing and pulling the push rod of the needle tube, so that the bone marrow can be circulated between the enrichment unit and the needle tube in multiple one-way cycles.

[0068] The present application aims to enrich new transplant materials using selective cell retention technology. A three-layer filtration structure combined with good enrichment materials is used internally to make the filtration more efficient and the enrichment ratio higher. At the same time, a controller is used to achieve controllable enrichment cycle times and enrichment time.

[0069] The present invention effectively overcomes various shortcomings of the prior art and has high industrial utilization value.

[0070] The above embodiments are merely illustrative of the principles and effects of the present application and are not intended to limit the present application. Anyone familiar with the technology may modify or change the above embodiments without violating the spirit and scope of the present application. Therefore, all equivalent modifications or changes made by a person of ordinary skill in the art without departing from the spirit and technical ideas disclosed in the present application shall still be covered by the claims of the present application.

Claims

1. A controllable stem cell enricher based on selective cell retention technology, characterized in that: include: The base is provided with: an enrichment unit, which has a filter screen therein, and is used to retain stem cells on the biological enrichment material placed on the filter screen when the bone marrow flows through; The enrichment unit comprises: An enrichment chamber formed by the first trough body on the base; wherein the enrichment chamber is hemispherical or funnel-shaped, and a discharge port is provided at the bottom; A chamber cover, used to cover the enrichment chamber, wherein a feed port is provided on the top of the chamber cover; The filter screen of the three-layer filter structure is horizontally arranged in the enrichment chamber, and is used to place the biological enrichment material thereon; wherein the gaps of each layer of the filter structure decrease from top to bottom; The feeding unit comprises a one-way valve three-channel and a needle tube loaded with bone marrow, and the needle tube is connected to the top and bottom of the enrichment unit respectively through a medical hose; the one-way valve three-channel is arranged on the base between the enrichment unit and the feeding unit, and comprises: The needle tube channel and the third slot for placing the needle tube in the same direction are used for directly inserting the needle connected to the needle tube; A feed channel, the outlet of which faces the upper surface of the base and is connected to the top of the enrichment unit by connecting a medical hose; A discharge channel, which is directly connected to the bottom of the enrichment unit or connected through a medical hose; A one-way valve is provided at the intersection of the channels, and is used to keep the feed channel open while the discharge channel is closed when the needle channel is subjected to a thrust from the needle tube; and to keep the feed channel closed while the discharge channel is opened when the needle channel is subjected to a suction from the needle tube; The driving unit is used to push and pull the push rod of the needle tube so that the bone marrow can flow between the enrichment unit and the needle tube in multiple one-way cycles.

2. The stem cell enricher according to claim 1, characterized in that: Driven by the driving unit, the bone marrow in the needle tube enters from the top of the enrichment unit, and the bone marrow that infiltrates to the bottom is sucked back into the needle tube from the bottom of the enrichment unit.

3. The stem cell enricher according to claim 1, characterized in that: The needle tube is installed in the third slot body on the base; wherein, the third slot body is provided with limited slots on both sides, which can be adapted to engage with the push handle of the needle tube to limit the displacement of the barrel of the needle tube.

4. The stem cell enricher according to claim 1, characterized in that: The enrichment chamber protrudes out of the upper plane of the base and is provided with a clamping portion; Wherein, one side of the clamping portion is hinged to one side of the cavity cover via a rotating shaft, so that the cavity cover can be flipped and closed relative to the clamping portion; A buckle assembly is provided on the other side of the clamping portion and the other side of the cavity cover, so as to lock and open the cavity cover and the clamping portion.

5. The stem cell enricher according to claim 1, characterized in that: The driving unit comprises: A motor, disposed in the second slot of the base, providing forward and reverse rotation forces; A transmission mechanism is coupled to the motor and the push rod of the needle tube respectively, and converts the rotational force driven by the motor into a force for axial displacement of the push rod and transmits it to the push rod; The controller is arranged on the mounting plate on the base and is electrically connected to the motor, and is used to set the rotation direction, speed and number of times of the motor.

6. The stem cell enricher according to claim 5, characterized in that: The transmission mechanism comprises: A transmission rod, with helical gears at both ends, one end of which is mounted on the second slot body to couple with the helical gear at the end of the rotating shaft of the motor, and the other end is inserted into the third slot body; A spiral cylinder is arranged in the third slot body, one end of which is provided with a spiral gear and coupled to the spiral gear of the transmission rod inserted into one end of the third slot body; the other end of which is provided with an internal thread sleeve; A threaded push rod is arranged in the third groove body, and its outer surface is provided with an external thread that matches the internal thread of the internal thread sleeve. One end of the threaded push rod is screwed into the internal thread sleeve, and the other end is provided with a fixing piece for detachably engaging the push rod tail of the needle tube.

7. The stem cell enricher according to claim 6, characterized in that: The second trough body is provided with a bearing base for fixing a first rolling bearing for the transmission rod to pass through; A second rolling bearing is embedded in one end of the third groove body, and a fixing rod is inserted into the third groove body. The fixing rod is used to be inserted into one end of the spiral cylinder provided with a spiral gear. A bearing fixing seat is provided on the inner side of the third groove body for fixing the third rolling bearing so as to allow the internal threaded sleeve to pass through.

8. The stem cell enricher according to claim 6, characterized in that: The fixing member comprises: a fixing portion welded to the other end of the threaded push rod; Slide blocks are arranged on both sides of the fixing portion; wherein, slide grooves adapted to the slide blocks are arranged on both sides of the third groove body; The supporting part is used for clamping and supporting the tail of the push rod of the needle tube.

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