Blood analyzer and automatic calibration measurement method for blood analyzer

Abstract

The present invention provides a blood analyzer and an automatic calibration measurement method for the blood analyzer. The blood analyzer includes: a sampling device having a pipette and a drive unit, the drive unit being used to drive the pipette to quantitatively draw a blood sample to be tested; a sample preparation device having at least one reaction pool and a reagent supply unit, wherein the blood sample to be tested drawn by the sampling device is mixed with a processing reagent provided by the reagent supply unit in the reaction pool to prepare a sample liquid to be tested; a detection device being used to detect the sample liquid to be tested to obtain routine blood parameters; and a control device being used to control the execution of the automatic calibration measurement process and the automatic retraction measurement operation of a test tube loaded with a calibration blood sample. With the blood analyzer and the automatic calibration measurement method of the embodiments of the present invention, the user only needs to place the test tube rack once and start a measurement once, and then does not need to participate again. The operation is simple, the degree of automation is high, and the efficiency of the calibration measurement is improved.

Description

Technical Field

[0001] The present invention generally relates to the field of sample analysis, and more particularly, to a blood analyzer and an automatic calibration measurement method for a blood analyzer. Background Art

[0002] During the use of medical instruments (e.g., blood analyzers, etc.), it is sometimes necessary to calibrate the instrument (e.g., before the instrument leaves the factory, before it is used again after repair, etc.). Currently, automatic calibration is a method for calibrating the performance of the instrument. This method performs calibration measurements in the following manner: Figure 1 , which shows a schematic diagram of a conventional device for calibrating and measuring medical instruments. First, the user places a test tube 11 containing a calibrant onto a test tube rack 12. The rack 12 is then placed onto a test tube rack loading station 13. The rack 12 is then moved to the sample inlet area for feeding. The rack 12 is then fed to the sample aspiration area, where the calibrant is mixed and sampled for testing. The rack 12 is then moved to the test tube rack unloading station 14, where the measurement results are displayed on the display interface. This completes a calibration measurement operation.

[0003] Typically, calibration measurements are performed a predetermined number of times (e.g., six times) to obtain a predetermined number of calibration results. Therefore, if the number of calibration results has not yet reached the predetermined number, the user must manually reposition the test tube rack containing the calibration sample onto the test tube rack loading station for the next measurement, until the predetermined number of calibration results has been obtained. During this process, the user must remain at the instrument's side, and after each measurement, the user must manually move the test tube rack to the test tube rack loading station. This repeated calibration process is cumbersome and requires user involvement throughout.

[0004] In view of the shortcomings of the existing automatic calibration measurement method for a blood analyzer, the art needs a new blood analyzer and an automatic calibration measurement method for a blood analyzer to solve the above problems. Summary of the Invention

[0005] The present invention is proposed to solve the above-mentioned problems. According to one aspect of the present invention, a blood analyzer is provided, comprising: a sampling device having a pipette with a pipette nozzle and a driving unit, the driving unit being used to drive the pipette to quantitatively draw a blood sample to be tested through the pipette nozzle; a sample preparation device having at least one reaction pool and a reagent supply unit, wherein the reaction pool is used to receive the blood sample to be tested drawn by the sampling device, the reagent supply unit supplies a processing reagent to the reaction pool, and the blood sample to be tested drawn by the sampling device and the processing reagent provided by the reagent supply unit are mixed in the reaction pool to prepare a sample liquid to be tested; a detection device being used to detect the sample liquid to be tested prepared by the sample preparation device to obtain routine blood parameters; and a control device being used to control the execution of an automatic calibration measurement process of the blood analyzer and to control the automatic retraction measurement operation of a test tube loaded with a calibration blood sample during the automatic calibration measurement process.

[0006] In one embodiment, the control device is further used to: determine whether the total number of calibration measurement results measured in the automatic fallback measurement operation reaches a preset threshold number of results; if the total number of calibration measurement results does not reach the threshold number of results, control the continuation of the automatic fallback measurement operation on the test tube; if the total number of calibration measurement results has reached the threshold number of results, control the termination of the automatic fallback measurement operation and the automatic calibration measurement process.

[0007] In one embodiment, the automatic retraction measurement operation includes: moving the test tube loaded with the calibration blood sample to the mixing position, performing a mixing operation on the calibration blood sample to mix the calibration blood sample; moving the test tube to the sample aspiration position, aspirating the calibration blood sample to test the aspirated sample, thereby obtaining a calibration measurement result for the aspirated sample; and automatically retracting the test tube to the mixing position for the next mixing, aspirating and testing, until the total number of calibration measurement results reaches the threshold number of results.

[0008] In one embodiment, the automatic calibration measurement process includes: providing a test tube loaded with the calibration blood sample, placing the test tube on a test tube rack, and placing the test tube rack at a loading position; moving the test tube rack to a sample injection area, and performing the automatic retract measurement operation on the calibration blood sample; after completing the automatic retract measurement operation, moving the test tube rack to an unloading position.

[0009] In one embodiment, the blood analyzer further includes a display device, wherein the result of the calibration measurement measured in the automatic fallback measurement operation is presented to the user through the display device so that the user can calibrate the blood analyzer based on the result of the calibration measurement and its corresponding target value.

[0010] In one embodiment, the reagent supply section includes: a first reagent supply section for supplying a white blood cell reagent, a second reagent supply section for supplying a red blood cell reagent, and a third reagent supply section for supplying a hemoglobin reagent; the detection device includes: an optical detection section for detecting a first sample liquid to be tested prepared by combining a portion of the blood sample to be tested and the white blood cell reagent supplied from the first reagent supply section to obtain white blood cell parameters and optionally platelet parameters, an impedance detection section for detecting a second sample liquid to be tested prepared by combining a portion of the blood sample to be tested and the red blood cell reagent supplied from the second reagent supply section to obtain red blood cell parameters and platelet parameters, and a colorimetric detection section for detecting a third sample liquid to be tested prepared by combining a portion of the blood sample to be tested and the hemoglobin reagent supplied from the third reagent supply section to obtain hemoglobin parameters.

[0011] In one embodiment, the results of the calibration measurement include routine blood parameters, which include one or more of the following parameters: white blood cell count, neutrophil percentage, lymphocyte percentage, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, red blood cell distribution width coefficient of variation, red blood cell distribution width standard deviation, red blood cell number, large red blood cell ratio, small red blood cell ratio, platelet distribution width standard deviation, mean platelet volume, large platelet ratio, high fluorescence intensity cell percentage, and mean corpuscular hemoglobin concentration.

[0012] According to another aspect of the present invention, an automatic calibration measurement method for a blood analyzer is provided, the method comprising: providing a test tube loaded with a calibration blood sample, placing the test tube on a test tube rack, and placing the test tube rack in a loading position; moving the test tube rack to a sample introduction area, performing an automatic retract measurement operation on the calibration blood sample; and moving the test tube rack to an unloading position when it is determined that the automatic retract measurement operation has been completed.

[0013] In one embodiment, determining that the automatic fallback measurement operation has been completed is based on the total number of calibration measurement results measured in the automatic fallback measurement operation reaching a preset threshold number of results.

[0014] In one embodiment, the automatic retraction measurement operation includes: moving the test tube loaded with the calibration blood sample to the mixing position, performing a mixing operation on the calibration blood sample to mix the calibration blood sample; moving the test tube to the sample aspiration position, aspirating the calibration blood sample to test the aspirated sample, thereby obtaining a calibration measurement result for the aspirated sample; and automatically retracting the test tube to the mixing position to perform the next mixing, aspirating and testing, until the total number of calibration measurement results reaches the threshold number of results.

[0015] In one embodiment, the method further comprises: presenting the calibration measurement result obtained in the automatic fallback measurement operation to a user, so that the user can calibrate the blood analyzer according to the calibration measurement result and its corresponding target value.

[0016] In one embodiment, the results of the calibration measurement include routine blood parameters, which include one or more of the following parameters: white blood cell count, neutrophil percentage, lymphocyte percentage, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, red blood cell distribution width coefficient of variation, red blood cell distribution width standard deviation, red blood cell number, large red blood cell ratio, small red blood cell ratio, platelet distribution width standard deviation, mean platelet volume, large platelet ratio, high fluorescence intensity cell percentage, and mean corpuscular hemoglobin concentration.

[0017] According to another aspect of the present invention, a computer-readable storage medium for a blood analyzer is provided, on which a computer program is stored, characterized in that when executed by a processor, the computer program implements the following steps: moving a test tube loaded with a calibration blood sample to a mixing position, performing a mixing operation on the calibration blood sample to mix the calibration blood sample; moving the test tube to a sample aspiration position, aspirating the calibration blood sample to test the aspirated sample, thereby obtaining a calibration measurement result for the aspirated sample; and automatically retracting the test tube to the mixing position for the next mixing, aspirating and testing, until the total number of calibration measurement results reaches the threshold number of results.

[0018] According to the blood analyzer, automatic calibration measurement method for the blood analyzer, and computer-readable medium of the embodiments of the present invention, the user only needs to place the test tube rack once and start the measurement once, and no further participation is required. The operation is simple, the degree of automation is high, and the efficiency of calibration measurement is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The above and other objects, features, and advantages of the present invention will become more apparent through a more detailed description of the embodiments of the present invention with reference to the accompanying drawings. The accompanying drawings are provided to provide a further understanding of the embodiments of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and are not intended to limit the present invention. In the drawings, the same reference numerals generally represent the same components or steps.

[0020] Figure 1 A schematic diagram of an existing device for calibrating and measuring medical instruments is shown;

[0021] Figure 2 shows a schematic structural block diagram of an example blood analyzer according to an embodiment of the present invention;

[0022] Figure 3 A schematic structural diagram of an optical detection unit of a detection device of an exemplary blood analyzer according to an embodiment of the present invention is shown;

[0023] Figure 4 A schematic structural diagram of an exemplary sheath flow impedance detection unit of an exemplary blood analyzer according to an embodiment of the present invention is shown;

[0024] Figure 5 shows a schematic structural block diagram of a control device of an exemplary blood analyzer according to an embodiment of the present invention;

[0025] Figure 6 A schematic diagram showing an example display interface of a display device of an example blood analyzer according to an embodiment of the present invention is shown;

[0026] Figure 7 A flowchart showing the steps of an exemplary automatic calibration measurement method for a blood analyzer according to an embodiment of the present invention is shown;

[0027] Figure 8 A flow chart illustrating exemplary steps of an automatic back-off measurement operation according to an embodiment of the present invention. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solutions and advantages of the present invention more apparent, exemplary embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments of the present invention, and it should be understood that the present invention is not limited to the exemplary embodiments described herein. Based on the embodiments of the present invention described in the present invention, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of the present invention.

[0029] As mentioned above, the existing automatic calibration process requires the user to manually take the test tube rack to the test tube rack loading station, and this calibration measurement is repeated multiple times, the operation is cumbersome, and the user's participation is required throughout the process.

[0030] In order to solve the above problems, the present invention provides a blood analyzer, which includes: a sampling device, having a pipette with a pipette nozzle and a driving unit, the driving unit being used to drive the pipette to quantitatively aspirate a blood sample to be tested through the pipette nozzle; a sample preparation device, having at least one reaction pool and a reagent supply unit, wherein the reaction pool is used to receive the blood sample to be tested aspirated by the sampling device, and the reagent supply unit provides a processing reagent to the reaction pool, and the blood sample to be tested aspirated by the sampling device and the processing reagent provided by the reagent supply unit are mixed in the reaction pool to prepare a sample liquid to be tested; a detection device, being used to detect the sample liquid to be tested prepared by the sample preparation device to obtain routine blood parameters; and a control device, being used to control the execution of an automatic calibration measurement process of the blood analyzer, and to control the automatic retraction measurement operation of a test tube loaded with a calibration blood sample during the automatic calibration measurement process.

[0031] The blood analyzer of the present invention only requires the user to place the test tube rack once and start measurement once, and does not need to participate in the subsequent operation. The operation is simple, the degree of automation is high, and the efficiency of calibration measurement is improved.

[0032] The blood analyzer and the automatic calibration measurement method for the blood analyzer according to the present invention are described in detail below with reference to specific embodiments.

[0033] Example 1

[0034] This embodiment provides a blood analyzer. Figure 2 , shows a schematic structural block diagram of a blood analyzer 100 according to this embodiment. As shown in the figure, the blood analyzer 100 may include a sampling device 110, a sample preparation device 120, a detection device 130 and a control device 140.

[0035] The sampling device 110 is used to quantitatively collect samples and transport the collected samples to the sample preparation device 120. Exemplarily, the sampling device 110 has a pipette with a pipette nozzle (e.g., a sampling needle) and a driving unit, which is used to drive the pipette to quantitatively absorb the blood sample to be tested through the pipette nozzle. For example, the sampling needle is driven by the driving unit to move to absorb the blood sample to be tested from a sample container (e.g., a test tube) containing the blood sample. Exemplarily, the sampling device 110 may also include a syringe and a sampling needle cleaning swab (not shown). Of course, the present invention is not limited to this, but can be configured as needed. For example, the sampling device 110 may also include an automatic sampler or a sample chamber, a blood separation valve or a quantitative pump, etc.

[0036] The sample preparation device 120 has at least one reaction cell 122 and a reagent supply device 124. The reagent supply device 124 is used to provide a processing reagent. It collects a fixed amount of reagent from a reagent bottle or reagent barrel and delivers it to the reaction cell 122. The reaction cell 122 is used to receive the blood sample to be tested drawn by the sampling device 110 and the processing reagent provided by the reagent supply device 124. The blood sample to be tested drawn by the sampling device 110 and the processing reagent provided by the reagent supply device 124 are mixed in the reaction cell to prepare a sample solution to be tested.

[0037] In some embodiments, the reagent supply device 124 may include a first reagent supply unit for supplying a white blood cell reagent, wherein the white blood cell reagent includes, for example, a hemolytic agent that can dissolve red blood cells in a blood sample and can distinguish different types of white blood cells, and optionally also includes a fluorescent reagent that can stain white blood cells. In some embodiments, the reagent supply device may also include a second reagent supply unit for supplying a red blood cell reagent, wherein the red blood cell reagent is, for example, a diluent. In other embodiments, the reagent supply device may also include a third reagent supply unit for supplying a hemoglobin reagent, wherein the hemoglobin reagent is, for example, a hemolytic agent that can dissolve red blood cells in a blood sample, release hemoglobin in the red blood cells, and convert hemoglobin into methemoglobin. In some embodiments, the white blood cell reagent and the hemoglobin reagent are the same hemolytic agent, that is, the first reagent supply unit and the third reagent supply unit are the same reagent supply unit.

[0038] Exemplarily, the reagent supply device 124 may further include a syringe and necessary pipe cleaning devices, etc., but is not limited thereto and may be configured as needed. For example, exemplarily, the reagent supply device may further include a metering pump and liquid reservoirs for various reagents.

[0039] Exemplarily, the reaction pools 122 may include multiple ones, each used for a different measurement mode. For example, the white blood cell classification measurement mode and the reticulocyte measurement mode usually use a sample reaction device respectively. This can improve the measurement rate and avoid cross-infection of reagents between different measurement modes.

[0040] Exemplarily, the sample preparation device 120 may further include a mixing device (not shown) for fully mixing the blood sample to be tested and the treatment reagent. Exemplarily, the mixing device may mix the blood sample to be tested and the treatment reagent by bubbling, and may include an air pump and a control valve device. Of course, the present invention is not limited to this, but may be configured as needed. For example, the mixing device may mix the sample and reagent by stirring with a motor, and may include any type of motor. It should be understood that, according to actual needs, the sample preparation device 120 may also include other devices, such as a temperature control device, etc., and the present invention is not limited to this.

[0041] The detection device 130 is used to detect the sample liquid prepared by the sample preparation device 120 to obtain routine blood parameters. For example, the detection device 130 may include multiple detection units, each for detecting different parameters, such as white blood cell parameters, red blood cell parameters, hemoglobin parameters, platelet parameters, etc.

[0042] Exemplarily, the detection device 130 may include an optical detection unit 131 , an impedance detection unit 132 , a colorimetry detection unit 133 , etc. Of course, the present invention is not limited thereto, and may be configured as needed.

[0043] The optical detection unit 131 is used to detect a first sample solution prepared from a portion of the blood sample to be tested and the leukocyte reagent supplied from the first reagent supply unit to obtain leukocyte parameters and optionally platelet parameters. Figure 3As shown, the optical detection unit 131 includes a light source 1311, a beam shaping assembly 1312, a flow cell 1313, and a forward scattered light detector 1314, arranged in a straight line. A dichroic mirror 1316 is arranged on one side of the flow cell 1313 at a 45° angle to the straight line. A portion of the side light emitted by blood cells in the flow cell 1313 passes through the dichroic mirror 1316 and is captured by a fluorescence detector 1315, which is arranged at a 45° angle behind the dichroic mirror 1316. Another portion of the side light is reflected by the dichroic mirror 1316 and captured by a side scattered light detector 1317, which is arranged at a 45° angle in front of the dichroic mirror 1316. Based on the forward scattered light signal captured by the forward scattered light detector 1314, the side scattered light signal captured by the side scattered light detector 1317, and the fluorescence signal captured by the fluorescence detector 1315, the white blood cells in the blood sample can be counted and classified. For example, the white blood cells can be classified into at least neutrophils, lymphocytes, and monocytes. Optionally, platelet parameters in the blood sample can be further detected, such as obtaining the platelet count.

[0044] The impedance detection unit 132 is used to detect the second sample solution prepared by a portion of the blood sample to be tested and the red blood cell reagent supplied from the second reagent supply unit to obtain red blood cell parameters and platelet parameters. For example, the impedance detection unit 132 is configured as a sheath flow impedance detection unit, such as Figure 4As shown, the sheath flow impedance detection unit 132 includes a flow chamber 1321 having a hole 1322 with a pair of electrodes 1323. The sheath flow impedance detection unit 132 detects the DC impedance generated when particles in the sample liquid to be tested pass through the hole 1322, and outputs an electrical signal reflecting the information when the particles pass through the hole. Specifically, after sucking the blood sample, the sampling device 110 is driven by its driving device and moves to the reaction pool 122 of the sample preparation device 120, and the sucked blood sample is injected into the reaction pool 122. The delivery pipeline transports the sample liquid to be tested after being treated with the diluent in the reaction pool 122 to the sheath flow impedance detection unit 132, that is, to the flow chamber 1321. The sheath flow impedance detection unit 132 can also be provided with a sheath liquid tank (not shown) for providing sheath liquid to the flow chamber 1321. In flow chamber 1321, the sample liquid to be tested flows under the sheath fluid. Holes 1322 transform the sample liquid flow into a thin stream, allowing particles (formed components) contained in the sample to be tested to pass through holes 1322 one by one. Electrodes 1323 are electrically connected to a DC power supply 1324, which supplies DC power between the pair of electrodes 1323. While DC power supply 1324 supplies DC power, the impedance between the pair of electrodes 1323 can be detected. A resistance signal representing the impedance change is amplified by amplifier 1325 and transmitted to control device 140. The magnitude of the resistance signal corresponds to the volume (size) of the particles. Therefore, by processing the resistance signal by control device 140, red blood cell parameters and platelet parameters of the sample liquid to be tested can be obtained.

[0045] In some embodiments, the detection device 130 further includes a colorimetric detection unit 133 for detecting a third sample solution prepared from a portion of the blood sample and a hemoglobin reagent supplied from the third reagent supply unit to obtain hemoglobin parameters.

[0046] In some embodiments, the control device 140 is configured to control the execution of an automatic calibration measurement process of the blood analyzer, and to control the automatic fallback measurement operation of a test tube containing a calibration blood sample during the automatic calibration measurement process. Specifically, the control device 140 may determine whether the total number of calibration measurement results measured during the automatic fallback measurement operation has reached a preset threshold number of results. If the total number of calibration measurement results has not reached the threshold number of results, the control device 140 may control the continuation of the automatic fallback measurement operation on the test tube; if the total number of calibration measurement results has reached the threshold number of results, the control device 140 may control the termination of the automatic fallback measurement operation and the automatic calibration measurement process.

[0047] Illustratively, the number of threshold results can be preset as needed, such as 6, 7, 8, etc., which is not limited in the present invention.

[0048] In some embodiments, as Figure 5As shown, the control device 140 includes at least a processing component 141, RAM 142, ROM 143, a communication interface 144, a memory 146, and an I / O interface 145. The processing component 141, RAM 142, ROM 143, the communication interface 144, the memory 146, and the I / O interface 145 communicate via a bus 147. The processing component can be a CPU, a GPU, or other chips with computing capabilities. The memory 146 contains various computer programs, such as an operating system and application programs, for execution by the processing component 141, as well as the data required to execute the computer programs. In addition, during the blood sample analysis process, any data that needs to be stored locally can be stored in the memory 146. The I / O interface 145 is composed of serial interfaces such as USB, IEEE 1394, or RS-232C, parallel interfaces such as SCSI, IDE, or IEEE 1284, and analog signal interfaces composed of D / A converters and A / D converters. The I / O interface 145 is connected to an input device consisting of a keyboard, mouse, touch screen, or other control buttons, and the user can use the input device to directly input data to the control device 140. In addition, the I / O interface 145 can also be connected to a display device (not shown) having a display function, such as an LCD screen, a touch screen, an LED display, etc. The control device 140 can output the processed data as image display data to the display device for display, such as analytical data, instrument operating parameters, etc. The communication interface 144 is an interface that can be any currently known communication protocol. The communication interface 144 communicates with the outside world via a network. The control device 140 can transmit data between any device connected to the network using a specific communication protocol through the communication interface 144.

[0049] Exemplarily, the automatic calibration measurement process may include providing a test tube loaded with a calibration blood sample, placing the test tube on a test tube rack, and placing the test tube rack in a loading position. The user can place any number of test tubes loaded with calibration blood samples as needed. For example, if the threshold number of results is preset to 6, one, two, three, or other test tubes can be placed. The more test tubes placed, the more results can be obtained each time, and thus the fewer times a rollback operation is required. However, since each test tube rack can only hold one test tube, the more test tubes placed, the more test tube racks will be required, and calibration samples will also be wasted. Therefore, in order to save calibration samples and test tube racks, the present invention can place only one test tube.

[0050] The automatic calibration measurement process also includes moving the test tube rack to the sample introduction area and automatically backtracking the measurement of the calibration blood sample. The number of automatic backtracking operations can be determined based on the preset threshold number of results and the number of calibration samples. For example, if the threshold number of results is 6, if there is 1 calibration sample, then the automatic backtracking operation will be performed 5 times, and if there are 2 calibration samples, then the automatic backtracking operation will be performed 2 times, thereby obtaining 6 calibration measurement results.

[0051] Illustratively, the automatic retraction measurement operation may include: moving a test tube containing the calibration blood sample to a mixing position, performing a mixing operation on the calibration blood sample, and mixing the calibration blood sample. Illustratively, a mixing gripper may be used to grasp the test tube containing the calibration blood sample and shake it thoroughly. Illustratively, the mixing operation time may be set as needed, such as 15 seconds, 30 seconds, or 1 minute.

[0052] Exemplarily, the automatic retraction measurement operation further includes: moving the test tube to the sample aspiration position, aspirating the calibration blood sample, testing the aspirated sample, and thereby obtaining calibration measurement results for the aspirated sample. The sample testing process may include, as described above, mixing the aspirated sample with various reagents supplied by the reagent supply units to prepare various test sample solutions, and testing the various test sample solutions to obtain various calibration measurement results.

[0053] Exemplarily, the results of the calibration measurement may include various routine blood parameters, wherein the routine blood parameters may include one or more of the following parameters: white blood cell count, neutrophil percentage, lymphocyte percentage, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, red blood cell distribution width coefficient of variation, red blood cell distribution width standard deviation, red blood cell number, large red blood cell ratio, small red blood cell ratio, platelet distribution width standard deviation, mean platelet volume, large platelet ratio, high fluorescence intensity cell percentage, mean corpuscular hemoglobin concentration.

[0054] Illustratively, the automatic retraction measurement operation further includes automatically retracting the test tube to the mixing position for the next mixing, sample aspiration, and detection operation, until the total number of calibration measurement results reaches a threshold number of results. Specifically, after obtaining the calibration measurement results, a determination is made as to whether the total number of calibration measurement results reaches a preset threshold number of results. If the threshold number of results has not been reached, the test tube is automatically retracted to the mixing position for the next mixing, sample aspiration, and detection operation.

[0055] For example, a waiting position may be provided after the sample aspiration position. In this case, the automatic retraction measurement operation may further include: after aspirating the sample at the sample aspiration position, moving the test tube to the waiting position to wait for the calibration measurement results. After obtaining the calibration measurement results, if the total number of calibration measurement results has not yet reached a threshold number of results, the test tube is automatically retracted from the waiting position to the mixing position to continue the next mixing, sample aspiration, and detection operations. If the total number of calibration measurement results has reached the threshold number of results, the test tube is moved from the waiting position to the unloading position to unload the test tube.

[0056] Exemplarily, the automatic calibration measurement process further includes: after the total number of calibration measurement results reaches a threshold number of results, that is, after the above-mentioned automatic fallback measurement operation is completed, moving the test tube rack to an unloading position to unload the test tube.

[0057] Exemplarily, the blood analyzer 100 may further include a display device 150 , wherein the result of the calibration measurement measured in the automatic fallback measurement operation is presented to the user via the display device 150 , so that the user can calibrate the blood analyzer 100 according to the result of the calibration measurement and its corresponding target value.

[0058] like Figure 6 A schematic diagram illustrates an exemplary display interface of an exemplary display device 150 according to an embodiment of the present invention. As shown, the display interface can display information such as the calibration date, batch number, blood sample parameter value, parameter average, original calibration coefficient, and new calibration coefficient. Of course, this is merely exemplary and the present invention is not limited thereto. For example, the display interface can also display information such as the batch number and expiration date.

[0059] According to the blood analyzer of this embodiment, the user only needs to place the test tube rack once and start a measurement once, and then does not need to participate again. The operation is simple, the degree of automation is high, and the efficiency of calibration measurement is improved.

[0060] Example 2

[0061] This embodiment provides an automatic calibration measurement method for a blood analyzer. Figure 7 , which shows a flowchart of the steps of an automatic calibration measurement method 700 for a blood analyzer according to an embodiment of the present invention.

[0062] like Figure 7 As shown, the automatic calibration measurement method 700 may include the following steps:

[0063] Step S720: providing a test tube loaded with a calibration blood sample, placing the test tube on a test tube rack, and placing the test tube rack at a loading position.

[0064] The user can place any number of test tubes containing calibration blood samples as needed, such as 1, 2, or 3. The more test tubes placed, the more results can be obtained each time, and the fewer times the backoff operation needs to be performed. However, since each test tube rack can only hold one test tube, the more test tubes placed, the more test tube racks will be required, and calibration samples will be wasted. Therefore, in order to save calibration samples and test tube racks, the present invention can only hold one test tube.

[0065] Step S740: Move the test tube rack to the sample injection area and perform an automatic back-off measurement operation on the calibration blood sample.

[0066] Among them, the number of automatic backoffs can be determined according to the preset number of threshold results and the number of calibration samples. For example, when the number of threshold results is 6, if the calibration sample is 1, it is required to automatically backoff 5 times; if the calibration samples are 2, it is required to automatically backoff 2 times, thereby obtaining 6 calibration measurement results.

[0067] Step S760: When it is determined that the automatic retraction measurement operation has been completed, the test tube rack is moved to the unloading position.

[0068] Specifically, determining whether the automatic fallback measurement operation has been completed can be based on whether the total number of calibration measurement results measured in the automatic fallback measurement operation has reached a preset threshold number of results. If the threshold number of results has been reached, the automatic fallback measurement operation is completed; if the threshold number of results has not been reached, the automatic fallback measurement operation continues.

[0069] refer to Figure 8 , which shows a flowchart of exemplary steps of an automatic fallback measurement operation according to an embodiment of the present invention. As shown in the figure, the exemplary steps of the automatic fallback measurement operation may include:

[0070] Step S7401: Move the test tube loaded with the calibration blood sample to the mixing position, and perform a mixing operation on the calibration blood sample to mix the calibration blood sample.

[0071] For example, a mixing clamp can be used to grab a test tube containing a calibration blood sample and shake it. For example, the time of the mixing operation can be set as needed, such as 15 seconds, 30 seconds, 1 minute, etc.

[0072] Step S7402: Move the test tube to the sample aspiration position, aspirate the calibration blood sample, and test the aspirated sample to obtain a calibration measurement result for the aspirated sample.

[0073] The process of testing the sample may be as described above: mixing the aspirated sample with various reagents supplied by the reagent supply units to prepare various test sample solutions, and testing the various test sample solutions to obtain various calibration measurement results.

[0074] Exemplarily, the results of the calibration measurement may include various routine blood parameters, wherein the routine blood parameters may include one or more of the following parameters: white blood cell count, neutrophil percentage, lymphocyte percentage, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, red blood cell distribution width coefficient of variation, red blood cell distribution width standard deviation, red blood cell number, large red blood cell ratio, small red blood cell ratio, platelet distribution width standard deviation, mean platelet volume, large platelet ratio, high fluorescence intensity cell percentage, mean corpuscular hemoglobin concentration.

[0075] Step S7403: The test tube is automatically returned to the mixing position for the next mixing, sampling and detection, until the total number of calibration measurement results reaches the threshold number of results.

[0076] Specifically, after obtaining the calibration measurement results, a determination is made as to whether the total number of calibration measurement results reaches a preset threshold number of results. If the total number of calibration measurement results has not yet reached the threshold number of results, the test tube is automatically retracted to the mixing position, and the next mixing, sampling, and testing operations are continued. If the total number of calibration measurement results has reached the threshold number of results, the automatic retraction measurement operation is completed, and the test tube is moved to the unloading position.

[0077] For example, a waiting position may be provided after the sample aspiration position. In this case, before step S7403, the following step may be included: after aspirating the sample at the sample aspiration position, the test tube is moved to the waiting position to wait for the calibration measurement results. After the calibration measurement results are obtained, if the total number of calibration measurement results has not yet reached the threshold number of results, the test tube is automatically returned from the waiting position to the mixing position to continue the next mixing, sample aspiration, and detection operations. If the total number of calibration measurement results has reached the threshold number of results, the test tube is moved from the waiting position to the unloading position to unload the test tube.

[0078] Exemplarily, the automatic calibration measurement method 700 may further include: presenting the calibration measurement result obtained in the automatic fallback measurement operation to the user, so that the user can calibrate the blood analyzer according to the calibration measurement result and its corresponding target value.

[0079] According to the automatic calibration measurement method for a blood analyzer of this embodiment, the user only needs to place the test tube rack once and start a measurement once, and does not need to participate again thereafter. The operation is simple, the degree of automation is high, and the efficiency of calibration measurement is improved.

[0080] Example 3

[0081] This embodiment provides a computer-readable storage medium for use in a blood analyzer. The computer-readable storage medium stores a computer program. When executed by a processor, the computer program can implement the following operations:

[0082] Move the test tube containing the calibration blood sample to the mixing position, and perform a mixing operation on the calibration blood sample to mix the calibration blood sample. For example, a mixing gripper can be used to grasp the test tube containing the calibration blood sample and shake it evenly. For example, the mixing operation time can be set as needed, such as 15 seconds, 30 seconds, or 1 minute.

[0083] The test tube is moved to the sample aspiration position, and the calibration blood sample is aspirated to test the aspirated sample, thereby obtaining calibration measurement results for the aspirated sample. The sample testing process can be as described above: the aspirated sample is mixed with various reagents supplied by various reagent supply units to prepare various test sample solutions, and the various test sample solutions are tested to obtain various calibration measurement results.

[0084] After obtaining the calibration measurement results, a determination is made as to whether the total number of calibration measurement results reaches a preset threshold number of results. If the total number of calibration measurement results has not yet reached the preset threshold number of results, the automatic retraction operation continues, automatically retracting the test tube to the mixing position for the next mixing, sample aspiration, and testing, until the total number of calibration measurement results reaches the threshold number of results. If the total number of calibration measurement results has reached the threshold number of results, the automatic retraction measurement operation is completed, and the test tube is moved to the unloading position for unloading.

[0085] For example, a waiting position can be provided after the sample aspiration position. After aspirating the sample at the sample aspiration position, the test tube can be moved to the waiting position to wait for the calibration measurement results. After the calibration measurement results are obtained, if the total number of calibration measurement results has not yet reached a threshold number of results, the test tube is automatically returned from the waiting position to the mixing position to continue the next mixing, sample aspiration, and detection operations. If the total number of calibration measurement results has reached the threshold number of results, the test tube is moved from the waiting position to the unloading position to unload the test tube.

[0086] Exemplarily, the results of the calibration measurement may include various routine blood parameters, wherein the routine blood parameters may include one or more of the following parameters: white blood cell count, neutrophil percentage, lymphocyte percentage, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, red blood cell distribution width coefficient of variation, red blood cell distribution width standard deviation, red blood cell number, large red blood cell ratio, small red blood cell ratio, platelet distribution width standard deviation, mean platelet volume, large platelet ratio, high fluorescence intensity cell percentage, mean corpuscular hemoglobin concentration.

[0087] Although example embodiments have been described herein with reference to the accompanying drawings, it should be understood that the above example embodiments are merely illustrative and are not intended to limit the scope of the present invention. Various changes and modifications may be made therein by those skilled in the art without departing from the scope and spirit of the present invention. All such changes and modifications are intended to be included within the scope of the present invention as claimed in the appended claims.

[0088] In the description provided herein, numerous specific details are described. However, it is understood that embodiments of the present invention may be practiced without these specific details. In some instances, well-known methods, structures, and techniques are not shown in detail so as not to obscure the understanding of this description.

[0089] Similarly, it should be understood that in order to streamline the present invention and aid in understanding one or more of the various inventive aspects, in the description of exemplary embodiments of the present invention, the various features of the present invention are sometimes grouped together into a single embodiment, figure, or description thereof. However, this approach to the present invention should not be interpreted as reflecting the intention that the claimed invention requires more features than those explicitly recited in each claim. More precisely, as reflected in the corresponding claims, the inventive point is that the corresponding technical problem can be solved with fewer features than all the features of a single disclosed embodiment. Therefore, the claims following the detailed description are hereby expressly incorporated into the detailed description, with each claim itself serving as a separate embodiment of the present invention.

[0090] It will be understood by those skilled in the art that, except where mutually exclusive, all features disclosed in this specification (including the accompanying claims, abstract, and drawings) and all processes or units of any method or apparatus disclosed herein may be combined in any combination. Unless expressly stated otherwise, each feature disclosed in this specification (including the accompanying claims, abstract, and drawings) may be replaced by an alternative feature providing the same, equivalent, or similar purpose.

[0091] Furthermore, those skilled in the art will appreciate that although some embodiments described herein include certain features included in other embodiments but not other features, combinations of features from different embodiments are intended to be within the scope of the present invention and to form different embodiments. For example, in the claims, any of the claimed embodiments may be used in any combination.

[0092] It should be noted that the above embodiments illustrate rather than limit the invention, and that those skilled in the art may devise alternative embodiments without departing from the scope of the appended claims. In the claims, any reference signs placed between brackets should not be construed as limiting the claims. The word "comprising" does not exclude the presence of elements or steps not listed in the claims. The word "a" or "an" preceding an element does not exclude the presence of a plurality of such elements. The present invention may be implemented by means of hardware comprising several different elements and by means of appropriately programmed computers. In a unit claim enumerating several means, several of these means may be embodied by the same item of hardware. The use of the words first, second, and third etc. does not indicate any order. These words may be interpreted as names.

[0093] The foregoing description is merely a specific embodiment of the present invention or an illustration of a specific embodiment. The scope of protection of the present invention is not limited thereto. Any modifications or substitutions that can be readily conceived by a person skilled in the art within the technical scope disclosed in the present invention are intended to be encompassed by the scope of protection of the present invention. The scope of protection of the present invention shall be subject to the scope of protection of the claims.

Claims

1. A blood analyzer, characterized in that: The blood analyzer comprises: A sampling device comprising a pipette with a pipette nozzle and a driving unit for driving the pipette to quantitatively draw a blood sample to be tested through the pipette nozzle; The sample preparation device comprises at least one reaction pool and a reagent supply unit, wherein the reaction pool is used to receive the blood sample to be tested drawn by the sampling device, and the reagent supply unit provides a processing reagent to the reaction pool. The blood sample to be tested drawn by the sampling device and the processing reagent provided by the reagent supply unit are mixed in the reaction pool to prepare a sample liquid to be tested; a detection device, used to detect the sample liquid prepared by the sample preparation device to obtain routine blood parameters; and a control device for controlling the execution of an automatic calibration measurement process of the blood analyzer and controlling an automatic retraction measurement operation of a test tube loaded with a calibration blood sample during the automatic calibration measurement process; The automatic fallback measurement operation includes: Moving the test tube loaded with the calibration blood sample to a mixing position, performing a mixing operation on the calibration blood sample to mix the calibration blood sample; Moving the test tube to a sample aspiration position, aspirating the calibration blood sample to detect the aspirated sample, thereby obtaining a calibration measurement result for the aspirated sample; and The test tube is automatically returned to the mixing position for the next mixing, sampling and detection.

2. The blood analyzer according to claim 1, wherein The control device is further configured to determine whether the total number of calibration measurement results obtained in the automatic fallback measurement operation reaches a preset threshold number of results, If the total number of results of the calibration measurement does not reach the threshold number of results, controlling to continue the automatic fallback measurement operation on the test tube; If the total number of results of the calibration measurement has reached the threshold number of results, control ends the automatic fallback measurement operation and the automatic calibration measurement process.

3. The blood analyzer according to claim 2, wherein: The automatic retraction measurement operation further includes: stopping the automatic retraction of the test tube to the mixing position until the total number of results of the calibration measurement reaches the threshold number of results.

4. The blood analyzer according to claim 1, wherein The automatic calibration measurement process includes: Providing a test tube loaded with the calibration blood sample, placing the test tube on a test tube rack, and placing the test tube rack at a loading position; moving the test tube rack to a sample introduction area and performing the automatic fallback measurement operation on the calibration blood sample; After completing the automatic retraction measurement operation, the test tube rack is moved to the unloading position.

5. The blood analyzer according to claim 3, wherein: The blood analyzer further includes a display device, wherein the result of the calibration measurement measured in the automatic fallback measurement operation is presented to a user through the display device so that the user can calibrate the blood analyzer according to the result of the calibration measurement and its corresponding target value.

6. The blood analyzer according to claim 1, wherein in, The reagent supply unit includes: a first reagent supply unit for supplying a white blood cell reagent, a second reagent supply unit for supplying a red blood cell reagent, and a third reagent supply unit for supplying a hemoglobin reagent; The detection device comprises: an optical detection unit for detecting a first sample solution prepared from a portion of the blood sample to be tested and a leukocyte reagent supplied from the first reagent supply unit to obtain leukocyte parameters and optionally platelet parameters, an impedance detection unit for detecting a second sample solution prepared from a portion of the blood sample to be tested and the red blood cell reagent supplied from the second reagent supply unit to obtain red blood cell parameters and platelet parameters; A colorimetric detection unit is used to detect a third sample solution prepared from a portion of the blood sample and a hemoglobin reagent supplied from the third reagent supply unit to obtain a hemoglobin parameter.

7. The blood analyzer according to any one of claims 2 to 6, wherein: The results of the calibration measurement include routine blood parameters, which include one or more of the following parameters: white blood cell count, neutrophil percentage, lymphocyte percentage, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, red blood cell distribution width coefficient of variation, red blood cell distribution width standard deviation, red blood cell number, large red blood cell ratio, small red blood cell ratio, platelet distribution width standard deviation, mean platelet volume, large platelet ratio, high fluorescence intensity cell percentage, and mean corpuscular hemoglobin concentration.

8. An automatic calibration measurement method for a blood analyzer, characterized in that: The method comprises: Providing a test tube loaded with a calibration blood sample, placing the test tube on a test tube rack, and placing the test tube rack at a loading position; moving the test tube rack to a sample introduction area and performing an automatic fallback measurement operation on the calibration blood sample; When it is determined that the automatic retraction measurement operation has been completed, moving the test tube rack to an unloading position; The automatic fallback measurement operation includes: Moving the test tube loaded with the calibration blood sample to a mixing position, performing a mixing operation on the calibration blood sample to mix the calibration blood sample; Moving the test tube to a sample aspiration position, aspirating the calibration blood sample to detect the aspirated sample, thereby obtaining a calibration measurement result for the aspirated sample; and The test tube is automatically returned to the mixing position for the next mixing, sampling and detection.

9. The method according to claim 8, wherein The determination that the automatic fallback measurement operation has been completed is based on the total number of calibration measurement results measured in the automatic fallback measurement operation reaching a preset threshold number of results.

10. The method according to claim 9, wherein The automatic fallback measurement operation includes: Moving the test tube loaded with the calibration blood sample to a mixing position, performing a mixing operation on the calibration blood sample to mix the calibration blood sample; Moving the test tube to a sample aspiration position, aspirating the calibration blood sample to detect the aspirated sample, thereby obtaining a calibration measurement result for the aspirated sample; and The test tube is automatically returned to the mixing position for the next mixing, sampling and detection, until the total number of results of the calibration measurement reaches the threshold number of results.

11. The method according to claim 9, wherein The method further includes presenting a result of the calibration measurement obtained in the automatic fallback measurement operation to a user, so that the user can calibrate the blood analyzer according to the result of the calibration measurement and its corresponding target value.

12. The method according to any one of claims 9 to 11, wherein The results of the calibration measurement include routine blood parameters, which include one or more of the following parameters: white blood cell count, neutrophil percentage, lymphocyte percentage, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, red blood cell distribution width coefficient of variation, red blood cell distribution width standard deviation, red blood cell number, large red blood cell ratio, small red blood cell ratio, platelet distribution width standard deviation, mean platelet volume, large platelet ratio, high fluorescence intensity cell percentage, and mean corpuscular hemoglobin concentration.

13. A computer-readable storage medium for a blood analyzer, wherein a computer program is stored on the computer-readable storage medium, characterized in that: When executed by a processor, the computer program implements the following steps: Moving the test tube loaded with the calibration blood sample to a mixing position, performing a mixing operation on the calibration blood sample to mix the calibration blood sample; Moving the test tube to a sample aspiration position, aspirating the calibration blood sample to test the aspirated sample, thereby obtaining a calibration measurement result for the aspirated sample; as well as After aspirating the sample, the test tube automatically returns to the mixing position to perform the next mixing, aspirating and detection until the total number of results of the calibration measurement reaches a threshold number of results.

Images