Method for operating a chemiluminescence analyzer
By introducing a bearing mechanism into the chemiluminescence analyzer, the reaction vessel completes the detection step on one bearing mechanism, solving the problems of complex structure and long sample processing time in the prior art, and achieving simplified equipment and efficient sample processing.
Patent Information
- Application Number
- CN202011432326.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Priority Date
- 2020-08-20
- Filing Date
- 2020-12-10
- Publication Date
- 2025-04-29
- Estimated Expiration
- 2040-12-10
AI Technical Summary
Due to the complex structure and long sample processing time of existing fully automatic photolass chemiluminescence detection devices, it is difficult to efficiently process fewer samples in small hospitals or small medical institutions, and the large diameter of the incubation disc leads to inaccurate positioning and structural component scheduling problems.
By introducing a load bearing mechanism into the chemiluminescence analyzer, the reaction vessel completes all steps from sample filling to luminescence detection on one load bearing mechanism, simplifying the detection process and structural design, reducing the equipment volume and weight, and simplifying the coordination between structural components.
The structure simplification of the chemiluminescence analyzer and the reduction of equipment volume and weight are achieved, and the coordination complexity and control errors between structural components are reduced. It is suitable for fully automatic chemiluminescence detection in small hospitals or small medical institutions.
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Figure CN114076734B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of chemiluminescence detection, and in particular to an operation method for a chemiluminescence analyzer. The present invention also provides a computer-readable storage medium. Background Art
[0002] Photochemiluminescence detection is a homogeneous immunoassay technology that is conducive to the rapid and sufficient completion of immune response.
[0003] Currently, fully automatic photochemiluminescence detection devices are usually used for the above detection. "Fully automatic" means that the working process of the detection device does not require human intervention or external intervention, which is conducive to ensuring the accuracy and efficiency of the detection process.
[0004] Existing detection devices for full-automatic photochemiluminescence detection are specially designed for large hospitals or medical institutions, and are used to detect as many samples as possible (for example, at least about 100) in the detection device at the same time. However, this usually requires the detection device to be provided with multiple (at least 2) incubation trays, which are generally arranged on the table in a dispersed manner. Although the detector can process a large number of samples at the same time, each sample must be rotated and moved a lot on the two incubation trays, and needs to be transferred between the two incubation trays. This makes the processing time for each sample relatively long. In addition, since the detector has many structural components, the coordination with each other is relatively complex and prone to errors. Therefore, this detector can only be applied to large hospitals that need to intensively process a large number of samples, while for small hospitals or other small medical institutions, if only relatively few samples (for example, less than 50) need to be processed, the efficiency is not high.
[0005] There are also schemes in the prior art that multiple reagent trays or incubation trays are coaxially arranged. However, in this scheme, the outermost tray has a very large diameter. This will lead to relatively poor stability in its operation, for example, it is easy to have inaccurate positioning problems. In this case, many structural components are more likely to have scheduling problems when they are fully automatically matched. Summary of the invention
[0006] The present invention proposes an operation method for a chemiluminescence analyzer, by which at least one problem in the above-mentioned prior art can be solved or at least alleviated. The present invention also proposes a computer-readable storage medium.
[0007] According to the present invention, a method for operating a chemiluminescence analyzer is proposed, the chemiluminescence analyzer includes a carrying mechanism, and the method includes the following steps: loading a first reaction container onto the carrying mechanism; adding a sample into the first reaction container; positioning the first reaction container in a reagent dispensing area, and adding a reagent into the first reaction container through at least one reagent dispensing mechanism; incubating the first reaction container on the carrying mechanism; and positioning the first reaction container at a light detection mechanism, and performing luminescence detection on the sample in the first reaction container.
[0008] In the above method of the present invention, in all steps from sample addition to luminescence detection, the reaction vessel is located on a carrying mechanism. The reaction vessel is rotated to different positions by the carrying mechanism to advance the detection process. Alternatively, the reaction vessel can be transferred to different positions on the carrying mechanism by a mechanical gripper to perform the detection step. Since the entire detection process can be carried out on a carrying mechanism, the structure of the chemiluminescence analyzer can be effectively simplified, allowing the volume and weight of the entire chemiluminescence analyzer to be greatly reduced. In addition, this also makes the coordination between the various structural components simpler and is not prone to errors and mistakes in control. This chemiluminescence analyzer is very suitable for fully automatic chemiluminescence detection in small hospitals or small medical institutions with less demand.
[0009] In one embodiment, in the step of loading the first reaction container onto the carrier, an empty accommodation position on the carrier is located at the reaction container allocating mechanism so that the first reaction container in the reaction container allocating mechanism can enter the empty accommodation position.
[0010] In one embodiment, in the step of adding the sample into the first reaction container, the first reaction container is placed in a sample dispensing area, and the sample is added into the first reaction container by a sample dispensing mechanism.
[0011] In one embodiment, in the step of adding the sample into the first reaction container, the sample dispensing mechanism first moves the sample to the sample position in the sample dispensing area, then positions the first reaction container at the sample position in the sample dispensing area, and adds reagents to the first reaction container through the sample dispensing mechanism.
[0012] In one embodiment, in the step of adding the sample into the first reaction container, the first reaction container is first located at the sample position in the sample distribution area, the sample distribution mechanism then moves the sample to the sample position in the sample distribution area, and adds reagents into the first reaction container.
[0013] In one embodiment, a plurality of sample positions are arranged in the sample distribution area, and the plurality of sample positions include a first sample position and a second sample position. The first reaction container is located at the first sample position to add a pre-sample into the first reaction container, and then the first reaction container is located in the reagent distribution area to add a diluent into the first reaction container to form a diluted sample. The first reaction container is then located at the second sample position, and the diluted sample in the first reaction container is added to the second reaction container located at the first sample position as a sample to be detected.
[0014] In one embodiment, in the step of adding a reagent into the first reaction container, the first reaction container is first located at a first reagent dispensing position in the reagent dispensing area to add a first reagent into the first reaction container, and then the first reaction container is located at a second reagent dispensing position in the reagent dispensing area to add a second reagent into the first reaction container.
[0015] In one embodiment, a plurality of second reagent dispensing positions are provided in the reagent dispensing area, and the first reaction container is placed in one of the second reagent dispensing positions to add the second reagent into the first reaction container.
[0016] In one embodiment, after adding the first reagent into the first reaction container and before adding the second reagent, the first reaction container on the supporting mechanism is incubated for a preset time period.
[0017] In one embodiment, in the step of adding reagents into the first reaction container, multiple first reagents are added into the first reaction container at the first reagent dispensing position, wherein the carrying mechanism moves to the first reagent dispensing position multiple times and adds one of the multiple first reagents each time it is at the first reagent dispensing position.
[0018] In one embodiment, in the step of adding reagents into the first reaction container, multiple first reagents are added into the first reaction container at the first reagent dispensing position, and during the addition of different first reagents, the carrying mechanism stays at the first reagent dispensing position.
[0019] In one embodiment, after adding at least one first reagent and / or second reagent into the first reaction container, the one carrying mechanism is oscillated to mix the sample and reagent in the first reaction container. When the one carrying mechanism is oscillating, other reaction containers are not subjected to adding samples, adding reagents and / or performing luminescence detection.
[0020] In one embodiment, the method further comprises: positioning the first reaction container in a reaction container removal zone, and removing the first reaction container from the accommodation position.
[0021] In one embodiment, the one carrier moves in only one direction to move the first reaction container to at least one of a reaction container dispensing area, a sample dispensing area, a reagent dispensing area, a luminescence detection area, and a reaction container removal area surrounding the one carrier.
[0022] In one embodiment, the chemiluminescent analyzer further comprises a control system for controlling at least one of the carrying mechanism, the reaction container dispensing mechanism, the sample dispensing mechanism, the reagent dispensing mechanism, the light detection mechanism and the reaction container removing mechanism.
[0023] In one embodiment, the chemiluminescence analyzer further comprises a control system, which receives information about the item to be detected and determines the time point when the reagent box carrying the required reagent in the reagent tray moves to the reagent dispensing position according to the information about the item to be detected.
[0024] In one embodiment, the carrying mechanism is configured as a rotatable carrying plate, and the carrying plate rotates around its center to move the first reaction container located in the receiving position.
[0025] According to a second aspect of the present invention, a computer-readable storage medium is provided, on which a computer program is stored, and the above-mentioned operation method is performed when the computer program is run. The storage medium can be, for example, a memory card, a USB flash drive, a CD-ROM, or a DVD. The storage medium can be fixedly arranged in the chemiluminescence analyzer, for example, as part of the control system of the chemiluminescence analyzer. Of course, the storage medium can also be used to transfer the computer program and temporarily connected to the chemiluminescence analyzer. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] The present invention will be described in more detail below with reference to the accompanying drawings, in which:
[0027] Figure 1 A schematic top view of an embodiment of a chemiluminescence analyzer according to the present invention is shown, which includes a carrying mechanism;
[0028] Figure 2 A schematic top view of another embodiment of a chemiluminescence analyzer according to the present invention is shown, which includes a supporting mechanism.
[0029] In the drawings, the same reference numerals are used for the same components. The drawings are not drawn to scale. DETAILED DESCRIPTION
[0030] The present invention will be further described below in conjunction with the accompanying drawings.
[0031] Figure 1 and Figure 2 A chemiluminescence analyzer (hereinafter referred to as "analyzer") 1 according to the present invention is schematically shown. The analyzer can be particularly used for fully automatic photochemiluminescence detection.
[0032] like Figure 1 and Figure 2 As shown, the analyzer 1 includes a frame 160, which is used to support other structural components of the analyzer 1. A rotatable carrying mechanism 70 is provided on the frame 160, which can rotate around its own center. The analyzer 1 may also include a sample distribution mechanism 20, a reaction vessel distribution mechanism 60, a reagent disk (for example, a reagent disk 110) for containing reagents to be used, at least one reagent distribution mechanism (for example, two reagent distribution mechanisms 80 and 120), a light detection mechanism 150 and a reaction vessel removal mechanism 140, which are arranged on the frame 160 and around the one carrying mechanism 70. By surrounding the arrangement of the carrying mechanism 70 with these structural components, corresponding regions can be formed at different parts of the carrying mechanism 70. For example, the corresponding part of the carrying mechanism 70 and the reaction vessel distribution mechanism 60 forms a reaction vessel distribution area A1. The corresponding part of the carrying mechanism 70 and the sample distribution mechanism 20 (and the reagent disk 110) forms a sample distribution area A2. The corresponding part of the carrying mechanism 70 and the reagent distribution mechanism 80, 120 forms a reagent distribution area A3. The portion of the carrying mechanism 70 corresponding to the light detection mechanism 150 forms a light emitting detection area A4. The portion of the carrying mechanism 70 corresponding to the reaction container removal mechanism 140 forms a reaction container removal area A5.
[0033] It should be understood that the above-mentioned regions do not change with the rotation of the supporting mechanism 70 .
[0034] The sample dispensing mechanism 20 includes a dispenser 23 for sucking liquid. The dispenser 23 can move relatively freely within a certain range. Within this range, a sample rack for placing samples can be set, such as Figure 1 The general sample rack 30 and the priority sample rack 40 in the dispenser. The priority sample rack can be used to place emergency samples, for example. In this range, a tip rack 50 for holding disposable tips can also be set. The tip rack 50 can also include a tip discarding area 51. In addition, the above-mentioned sample distribution area A2 is also within the movement range of the dispenser.
[0035] In the sample distribution area A2, a plurality of sample positions may be provided to obtain the diluted sample to be tested. In the following, the method will be described in more detail.
[0036] The analyzer 1 also includes a first reagent dispensing mechanism 120 and a second reagent dispensing mechanism 80. The first reagent dispensing mechanism 120 can be used to distribute a reagent specifically used for a single test item, which is referred to as the first reagent in this article. The second reagent dispensing mechanism 80 can be used to distribute a reagent that is required to be used for each test item, which is referred to as the second reagent in this article. It should be pointed out here that the aforementioned first reagent and the second reagent are only used to illustrate the role of the first and second reagent dispensing mechanisms, and should not be understood as a restriction on the use of the first reagent and the second reagent themselves. The first reagent and the second reagent can be configured according to the needs of the user.
[0037] See also Figure 2 , a first reagent dispensing position L1 is formed in the area where the carrying mechanism 70 overlaps with the reagent dispensing path of the first reagent dispensing mechanism 120. Correspondingly, a first reagent acquisition position is formed in the area where the reagent disk 110 overlaps with the reagent dispensing path of the first reagent dispensing mechanism 120, for example, there are a plurality of first reagent acquisition positions L4 and L5. When the carrying mechanism 70 rotates to the first reaction container at the first reagent dispensing position L1, the first reagent dispensing mechanism 120 acquires the corresponding first reagent from at least one of the first reagent acquisition positions L4 and L5, and then moves it to the first reagent dispensing position L1, and adds the first reagent to the first reaction container.
[0038] In addition, a second reagent dispensing position is formed in the area where the reagent dispensing path of the carrying mechanism 70 overlaps with the reagent dispensing path of the second reagent dispensing mechanism 80, for example, there are a plurality of second reagent dispensing positions L2 and L3. Correspondingly, a second reagent acquisition position L6 is formed in the area where the reagent disk 110 overlaps with the reagent dispensing path of the second reagent dispensing mechanism 80. When the carrying mechanism 70 rotates to the point where the first reaction container is in one of the second reagent dispensing positions L2 and L3, the second reagent dispensing mechanism 80 acquires the corresponding second reagent from the second reagent acquisition position L6, and then moves it to the corresponding first reagent dispensing position to add the second reagent into the first reaction container.
[0039] In a preferred embodiment, a cleaning mechanism 130 for cleaning the reagent dispenser is provided between the reagent disk 110 and the supporting mechanism 70 .
[0040] In addition, if Figure 1 and Figure 2As shown, the analyzer 1 includes a control module 10. The control module 10 can be used to control the working process of each structural component of the analyzer 1 to implement the entire detection method (described in detail below). For example, the user can control the control module 10 (mid-position computer) through another computer (host computer) to implement the above-mentioned working process. The computer and the control module 10 are configured with a storage medium for storing a computer program for executing the operating method of the analyzer 1 and the supporting mechanism 70.
[0041] As an alternative, the user may also directly operate the control module 10 to implement the above working process.
[0042] In addition, if Figure 1 and Figure 2 As shown, a flow path module 90 is arranged at the upper right of the entire analyzer 1. The flow path module 90 can be used, for example, to provide cleaning fluid to the cleaning mechanism 130 and discharge waste liquid in the cleaning mechanism 130, thereby ensuring the accuracy of detection.
[0043] The following will be combined Figure 1 and Figure 2 The operating method of the analyzer 1 and the supporting mechanism 70 therein according to the present invention is described below. The operating method may be an operating method for realizing fully automatic photochemiluminescence detection.
[0044] For a single detection process, first, the first reaction container is loaded onto the carrier mechanism 70, and a sample is added into the first reaction container.
[0045] Specifically, in this step, the distributor 23 of the sample dispensing mechanism 20 first moves to the tip rack 50, so that the disposable tips on the tip rack 50 are installed on the distributor 23. Then, the distributor 23 is moved to the sample rack (including the general sample rack 30 and the priority sample rack 40), and the corresponding sample is collected there. Thereafter, the distributor 23 carries the sample and moves to the sample distribution area A2 of the carrier mechanism 70, for example, it can be positioned at a predetermined position. Corresponding to this action, the reaction container dispensing mechanism 60 loads the first reaction container into the empty accommodation position on the carrier mechanism 70, which is located in the reaction container distribution area A1. The carrier mechanism 70 rotates, so that the first reaction container rotates from the reaction container distribution area A1 to a predetermined position in the sample distribution area A2, which is located below the distributor 23. Thus, the distributor 23 can add the sample to the first reaction container.
[0046] In addition, the sample added to the first reaction container from the sample rack can be a pre-sample. For example, the carrier mechanism 70 can first be rotated until the first reaction container is located at the first sample position, so that the pre-sample (i.e., the undiluted sample) from the sample rack can be added to the first reaction container through the sample distribution mechanism 20. Then, the carrier mechanism is rotated until the first reaction container is located in the reagent distribution area A3, so that the reagent as a diluent is added to the first reaction container, thereby obtaining a diluted sample in the first reaction container. Thereafter, the carrier mechanism 70 can be rotated again until the first reaction container is located at the second sample position, and the diluted sample in the first reaction container is added to another empty reaction container located at the first sample position at this time through the sample distribution mechanism 20 as a sample to be detected. Thus, an independent sample dilution device can be omitted.
[0047] It should be understood that, according to the dilution multiple required, multiple dilution operations may be performed between the first sample position and the second sample position to obtain the sample to be tested.
[0048] After the sample is added to the first reaction container, the carrier mechanism 70 is further rotated to move the first reaction container from the sample dispensing area A2 to the reagent dispensing area A3 , and the corresponding reagent is added to the first reaction container through the reagent dispensing mechanisms 80 , 120 .
[0049] Specifically, in this step, the carrier mechanism 70 rotates so that the first reaction container moves to the first reagent allocation position L1 in the reagent allocation area A3. At the same time, the reagent disc 110 sends the first reagent to be added to the first reagent acquisition position L4 or L5. Thus, the first reagent allocation mechanism 120 can obtain the corresponding first reagent from the first reagent acquisition position L4 or L5 and add it to the first reaction container at the first reagent allocation position L1. Thereafter, the carrier mechanism 70 continues to rotate so that the liquid in the first reaction container is heated and incubated, and other reaction containers move to corresponding positions and perform corresponding operation steps. After a period of time, the carrier mechanism 70 rotates to the above-mentioned first reaction container and returns to the first reagent allocation position L1. Correspondingly, the reagent disc 110 sends the first reagent to be added to the first reagent acquisition position L4 or L5. Thus, the first reagent allocation mechanism 120 can obtain the other first reagent from the first reagent acquisition position L4 or L5 and add it to the first reaction container at the first reagent allocation position L1. It should be understood that between two processes of adding different first reagents through the first reagent dispensing mechanism 120 , the first reagent dispensing mechanism 120 may be cleaned by the cleaning mechanism 130 .
[0050] Alternatively, during the addition of different first reagents, the carrier mechanism 70 may not rotate. In other words, when the first reaction container on the carrier mechanism 70 is in the first reagent dispensing position within a predetermined time, the first reagent dispensing mechanism adds the required different first reagents into the first reaction container.
[0051] After that, the carrier mechanism 70 rotates again for a period of time (for example, the carrier mechanism rotates 6, 8 or 12 times, etc.). During this period, the liquid in the first reaction container is heated and incubated by the carrier mechanism 70, and the other reaction containers of the carrier mechanism 70 can be moved to the corresponding position and perform the corresponding operation steps. After that, the carrier mechanism 70 rotates until the first reaction container moves to the second reagent allocation position L2 or L3. The selection of the second reagent allocation position depends on the detection project information. After the analyzer obtains the detection project information, the analyzer obtains the duration of the detection project and selects the second reagent allocation position according to the corresponding detection project duration. For example, the second reagent allocation position L2 can be used for conventional project detection with a detection time of 20 minutes, and the second reagent allocation position L3 can be used for rapid project detection with a detection time of 15 minutes. Thus, by selecting the second reagent allocation position, the detection project conflict caused by the same time and the same moving path is avoided. In conventional analyzers, the rotation speed and transportation distance of the reaction disk are fixed. If a rapid detection project (such as an emergency project) is to be inserted, it is necessary to stop or delay the subsequent detection project. However, in the analyzer of the present invention, by setting a plurality of second reagent allocation positions, flexible detection time matching can be achieved without disturbing or rarely disturbing the progress of other items to be tested.
[0052] At the same time, the reagent tray 110 delivers the second reagent to be added to the second reagent acquisition position L6. Thus, the second reagent dispensing mechanism 80 can obtain the corresponding second reagent from the second reagent acquisition position L6 and add it to the first reaction container at the second reagent dispensing position L2 or L3.
[0053] Here, reaction container and reagent dispensing mechanism 120, 80 can arrive at corresponding position simultaneously.As an alternative, can after reaction container arrives at specific position, corresponding reagent dispensing mechanism 120, 80 moves to this specific position again, and vice versa.
[0054] After adding one or more reagents, the first reaction container containing the sample and reagents is incubated for a predetermined time on the carrier mechanism 70. During the incubation of the first reaction container, the first reaction container is not operated at the corresponding mechanism, for example, no sample / reagent is added, and no luminescence detection is performed.
[0055] When the first reaction container is incubated, the carrier 70 may vibrate, thereby facilitating mixing of the sample and reagent in the first reaction container. The vibration may be a rotational vibration of the carrier 70, for example, generated by a small but high-frequency reciprocating rotation of the carrier 70.
[0056] It should be understood that when the first reaction container is incubated, other empty accommodation positions on the carrier mechanism can receive additional reaction containers, and the additional reaction containers can also perform the steps of adding samples and reagents, luminescence detection and removal. Preferably, when the carrier mechanism 70 vibrates, these additional reaction containers do not perform the operations of adding samples and reagents, luminescence detection and removal at the same time to avoid problems such as spilling of samples and reagents, mutual interference between structural components, and operation failure.
[0057] After the first reaction container is incubated, the carrier mechanism 70 is rotated to move the first reaction container to the luminescence detection area A4 and to a position below the light detection mechanism 150. Thus, the light detection mechanism 150 can perform luminescence detection on the sample in the first reaction container.
[0058] After the luminescence detection, the carrier mechanism 70 is further rotated to move the first reaction container to the reaction container removal area A5, where the first reaction container is removed from the accommodation position by the reaction container removal mechanism 140, leaving an empty accommodation position for subsequent use. In a preferred embodiment, after the first reaction container is subjected to luminescence detection, the carrier mechanism 70 is rotated until the first reaction container is directly moved to the reaction container removal area A5.
[0059] In the process of performing the above steps, the carrying mechanism 70 preferably rotates only in one direction (rotational vibration can be ignored), for example, counterclockwise, so that the first reaction container can be moved between the reaction container distribution area A1, the sample distribution area A2, the reagent distribution area A3, the luminescent detection area A4 and the reaction container removal area A5. Thus, the manipulation of the carrying mechanism is simplified. Through the aforementioned manipulation steps of the present application, combined with the unidirectional rotation of the carrying mechanism, extremely flexible arrangement of detection items can also be achieved, especially the mixing of conventional time-length detection items and rapid detection items.
[0060] The above steps may be performed continuously, or operation steps of other reaction containers may be inserted between them. For example, after adding the sample to the first reaction container and before adding the reagent, the sample or reagent may be added to another reaction container or other operations may be performed.
[0061] When the first reaction container is not transferred to the corresponding operation position (e.g., the first reagent dispensing position), other reaction containers on the carrier mechanism 70 may be located at the corresponding operation position to perform corresponding operations (e.g., adding samples, adding reagents, performing luminescence detection, etc.). Alternatively, when the first reaction container is at the corresponding operation position, other reaction containers may also be located at other operation positions to perform different operations simultaneously.
[0062] Thus, the first reaction container can complete a test during the process of the carrier mechanism 70 rotating multiple times. During this process, the first reaction container can be sufficiently incubated, which is beneficial to the accuracy and effectiveness of the test. At the same time, other reaction containers can also be operated in a timely and effective manner, so that multiple (for example, 50) reaction containers can be operated on the carrier mechanism 70 at the same time. Thus, the position of the entire carrier mechanism can be utilized to the greatest extent, so that the carrier mechanism 70 can achieve high-throughput detection while being compact in structure.
[0063] By using the above method, the analyzer 1 can realize fully automatic photochemiluminescence detection, which can simplify the scheduling and control relationship between various structural components as much as possible, making the detection process more efficient and faster, and less prone to mistakes or errors.
[0064] Herein, the reaction container may be, for example, a test tube or a reaction cup.
[0065] Although the present invention has been described with reference to preferred embodiments, various modifications may be made thereto and parts thereof may be replaced by equivalents without departing from the scope of the present invention. In particular, the various technical features mentioned in the various embodiments may be combined in any manner as long as there are no structural conflicts. The present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.
Claims
1. A method for operating a chemiluminescent analyzer, the chemiluminescent analyzer comprising a carrying mechanism, the method comprising the following steps: loading a first reaction container onto the carrying mechanism; In the step of adding the sample into the first reaction container, the first reaction container is positioned in a sample dispensing area, and the sample is added into the first reaction container by a sample dispensing mechanism, wherein a plurality of sample positions are arranged in the sample dispensing area, and the plurality of sample positions include a first sample position and a second sample position, the first reaction container is positioned in the first sample position to add a pre-sample into the first reaction container, and then the first reaction container is positioned in a reagent dispensing area to add a diluent into the first reaction container to form a diluted sample, and then the first reaction container is positioned in the second sample position, and the diluted sample in the first reaction container is added into the second reaction container positioned at the first sample position as a sample to be detected; The first reaction container is placed in a reagent dispensing area, and a reagent is added into the first reaction container by at least one reagent dispensing mechanism; Incubating the first reaction container on the supporting mechanism; and The first reaction container is placed at a light detection mechanism, and luminescence detection is performed on the sample in the first reaction container.
2. The operating method for a chemiluminescence analyzer according to claim 1, characterized in that: In the step of loading the first reaction container onto the carrier, an empty receptacle on the carrier is positioned at the reaction container allocating mechanism so that the first reaction container in the reaction container allocating mechanism can enter the empty receptacle.
3. The operating method for a chemiluminescence analyzer according to claim 1, characterized in that: In the step of adding the sample into the first reaction container, the sample dispensing mechanism first moves the sample to the sample position in the sample dispensing area, then positions the first reaction container at the sample position in the sample dispensing area, and adds reagents to the first reaction container through the sample dispensing mechanism.
4. The operating method for a chemiluminescence analyzer according to claim 1, characterized in that: In the step of adding the sample into the first reaction container, the first reaction container is first located at the sample position in the sample distribution area, the sample distribution mechanism then moves the sample to the sample position in the sample distribution area, and adds reagents into the first reaction container.
5. The operating method for a chemiluminescence analyzer according to any one of claims 1 to 4, characterized in that: In the step of adding a reagent into the first reaction container, the first reaction container is first positioned at a first reagent dispensing position in the reagent dispensing area to add a first reagent into the first reaction container, and then the first reaction container is positioned at a second reagent dispensing position in the reagent dispensing area to add a second reagent into the first reaction container.
6. The operating method for a chemiluminescence analyzer according to claim 5, characterized in that: A plurality of second reagent dispensing positions are arranged in the reagent dispensing area, and the first reaction container is placed in one of the second reagent dispensing positions to add the second reagent into the first reaction container.
7. The operating method for a chemiluminescence analyzer according to claim 5, characterized in that: After adding the first reagent into the first reaction container and before adding the second reagent, the first reaction container on the carrying mechanism is incubated for a preset time period.
8. The operating method for a chemiluminescence analyzer according to claim 5, characterized in that: In the step of adding reagents into the first reaction container, multiple first reagents are added into the first reaction container at the first reagent dispensing position, wherein the carrying mechanism moves to the first reagent dispensing position multiple times and adds one of the multiple first reagents each time at the first reagent dispensing position.
9. The operating method for a chemiluminescence analyzer according to claim 5, characterized in that: In the step of adding reagents into the first reaction container, a plurality of first reagents are added into the first reaction container at the first reagent dispensing position, and during the addition of different first reagents, the carrying mechanism stays at the first reagent dispensing position.
10. The operating method for a chemiluminescence analyzer according to any one of claims 1 to 4, characterized in that: After adding at least one first reagent and / or second reagent into the first reaction container, the one carrying mechanism is oscillated to mix the sample and reagent in the first reaction container. When the one carrying mechanism is oscillating, no sample, reagent or luminescence detection is performed in other reaction containers.
11. The operating method for a chemiluminescence analyzer according to any one of claims 1 to 4, characterized in that: The method further comprises: Position the first reaction container in a reaction container removal zone, and remove the first reaction container from the receiving position.
12. The operating method for a chemiluminescence analyzer according to any one of claims 1 to 4, characterized in that: The one carrier moves in only one direction to move the first reaction container to at least one of a reaction container dispensing area, a sample dispensing area, a reagent dispensing area, a luminescence detection area, and a reaction container removal area surrounding the one carrier.
13. The operating method for a chemiluminescence analyzer according to any one of claims 1 to 4, characterized in that: The chemiluminescent analyzer further comprises a control system for controlling at least one of the carrying mechanism, the reaction container dispensing mechanism, the sample dispensing mechanism, the reagent dispensing mechanism, the light detection mechanism and the reaction container removing mechanism.
14. The operating method for a chemiluminescence analyzer according to any one of claims 1 to 4, characterized in that: The chemiluminescence analyzer also includes a control system, which receives information about items to be detected and determines the time point when the reagent box carrying the required reagents in the reagent tray moves to the reagent dispensing position according to the information about the items to be detected.
15. The operating method for a chemiluminescence analyzer according to any one of claims 1 to 4, characterized in that: The carrying mechanism is configured as a rotatable carrying plate, and the carrying plate rotates around its center to move the first reaction container located in the accommodating position. 16 . A computer-readable storage medium having a computer program stored thereon, wherein the execution of the computer program causes the execution of the operating method according to claim 1 .
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