Bioluminescence detector

Abstract

The utility model relates to the technical field of biological detection, and discloses a bioluminescence detector which comprises a bottom plate, a reagent rack and a luminescence detection module, the reagent rack and the luminescence detection module are arranged on the bottom plate, the reagent rack is connected with a kit in a sliding manner, a light filtering assembly is arranged above the kit, the light filtering assembly is provided with a plurality of light filters for selecting light waves, and the light filtering assembly is connected with the luminescence detection module. The luminous detection module detects an optical signal in the kit through the optical filter; the whole structure is compact, optical signals of non-target wavelengths can be removed and background light interference can be reduced by using the optical filters, so that the purity of the detection signals is improved, the light emitting detection module can detect the optical signals of the target wavelengths in a more targeted manner, and the accuracy of the detection result is enhanced; the appropriate filtering wave band can be selected according to different types of chemical agent reactions to meet different detection requirements, the application range of the detector is expanded, and the detector can be suitable for various bioluminescence reactions.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to a bioluminescence detector. Background Art

[0002] Bioluminescence detection technology is a technique for detecting signals from biological samples based on the luminescence phenomenon of chemical reactions. It is widely used in life sciences, medical diagnostics, drug screening, food safety testing, and environmental monitoring. Bioluminescence reactions do not require external light sources for stimulation. Chemical substances added to biological samples react under specific conditions to produce light signals, which are captured and quantitatively analyzed by the instrument using highly sensitive photodetectors. Due to its high sensitivity, bioluminescence detection technology has become a standard detection method in many laboratories and diagnostic equipment.

[0003] Most of the biochemiluminescence detectors currently used in the market use highly sensitive photomultiplier tubes (PMTs) or CCD sensors as detection components, which can sensitively capture weak luminescence signals. However, the optical systems of the aforementioned detection components are more sensitive to light signals within a wider spectral range and cannot effectively distinguish signals of specific wavelengths from other interfering signals. Moreover, different chemiluminescence reactions involve different luminescence wavelength ranges, which will increase background noise for light signals of non-target wavelengths, making it difficult for the detector to detect the target signal in a targeted manner, thereby affecting the accuracy of the detection results.

[0004] Therefore, it is necessary to provide a bioluminescence detector to solve the problem that the above detectors are difficult to detect target signals in a targeted manner. Utility Model Content

[0005] The purpose of the present invention is to provide a bioluminescence detector to solve the technical problems mentioned in the background technology.

[0006] The utility model adopts the following technical solutions:

[0007] A bioluminescence detector comprises a base plate, a reagent rack and a luminescence detection module disposed on the base plate, wherein the reagent rack is slidably connected to a reagent kit, a filter assembly is disposed above the reagent kit, and the luminescence detection module is disposed above the filter assembly;

[0008] The optical filter assembly is provided with a plurality of optical filters for selecting light waves, and the luminescence detection module detects the optical signal in the reagent kit through the optical filters.

[0009] Furthermore, a transport mechanism is provided on the base plate, and the transport mechanism includes a first guide rail, which is provided on the upper end surface of the base plate. The first guide rail is slidably connected to a slider, and the slider is fixedly connected to the reagent rack. The slider is connected to the driving end of the driving assembly to drive the reagent rack to slide along the first guide rail.

[0010] Furthermore, the drive assembly includes a first motor fixedly connected to the base plate, the driving end of the first motor is connected to a first synchronous wheel, a second synchronous wheel is provided at an end of the first guide rail away from the first motor, and the outer sides of the first synchronous wheel and the second synchronous wheel are connected by a belt, and the belt is fixedly connected to the slider.

[0011] Furthermore, a shell is provided on the upper end surface of the bottom plate, a detection cavity for accommodating the reagent rack is provided in the shell, and the filter assembly and the luminescence detection module are respectively fixedly connected to the shell.

[0012] Furthermore, a guide plate is fixedly connected inside the shell, and the guide plate is provided with a guide portion for the reagent rack to slide in and out, so that the guide plate is slidably connected to the reagent rack.

[0013] Furthermore, the filter assembly includes a second motor and a filter wheel, the second motor is arranged on the upper end surface of the shell, the filter wheel is rotatably connected to the inner top wall of the shell, the output shaft of the second motor penetrates the shell and is connected to the filter wheel, and a plurality of light-transmitting holes are opened in the axial direction of the filter wheel, and the filter is arranged in the light-transmitting holes.

[0014] Furthermore, the shell is open at one end away from the first motor, and a connecting plate is provided at the opening of the shell. The connecting plate is provided with an avoidance portion for the reagent rack to slide in and out. The end surface of the connecting plate away from the first motor is provided with a second guide rail, and a sealing door is slidably connected to the second guide rail.

[0015] Furthermore, a third motor is provided on one side of the connecting plate, the output shaft of the third motor is connected to a gear, and a rack meshing with the gear is provided on one side of the sealing door, so that the sealing door slides along the second guide rail through the drive of the third motor.

[0016] Furthermore, a pressure plate is provided on the side of the slider close to the belt, the bottom end of the pressure plate abuts against the belt, and the bottom end of the pressure plate is fixedly connected to a bite plate, which bites and fixes the belt to fix the slider to the belt.

[0017] Beneficial effects:

[0018] The utility model provides a bioluminescence detector, which has a compact overall structure by slidingly placing a reagent kit in a reagent rack and arranging a filter assembly and a luminescence detection module above the reagent kit. The reagent kit and the filter are easy and convenient to replace. By using the filter, light signals of non-target wavelengths can be removed, and background light interference can be reduced, thereby improving the purity of the detection signal, enabling the luminescence detection module to detect the target wavelength light signal more specifically, thereby enhancing the accuracy of the detection results. In addition, the filter assembly includes multiple filters, which can select appropriate filter bands according to different types of chemical agent reactions to meet different detection needs, thereby expanding the application range of the detector and making it applicable to a variety of bioluminescence reactions. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the overall structure of a bioluminescence detector of the present utility model;

[0020] Figure 2 This is a schematic diagram of the structure of a bioluminescence detector without the outer shell of the utility model;

[0021] Figure 3 This is a schematic structural diagram of the filter assembly of the present utility model;

[0022] Figure 4 This is a schematic structural diagram of the transport mechanism of the present invention;

[0023] Figure 5 This is a structural diagram of the sealing door of the present invention.

[0024] Among them: 1. Base plate; 2. Reagent rack; 3. Reagent kit; 4. Filter assembly; 41. Filter; 42. Second motor; 43. Filter wheel; 5. Luminescence detection module; 6. Transport mechanism; 61. First guide rail; 62. Slider; 63. First motor; 64. First synchronous wheel; 65. Second synchronous wheel; 66. Belt; 7. Housing; 8. Guide plate; 9. Connecting plate; 10. Second guide rail; 11. Sealing door; 12. Third motor; 13. Gear; 14. Rack; 15. Pressing plate; 16. Engraving plate.

[0025] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0026] It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0027] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the said features. In the description of the present invention, "several" means two or more, unless otherwise clearly and specifically defined.

[0028] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integral connections; they may refer to mechanical connections, direct connections, or indirect connections through an intermediate medium; they may refer to internal communication between two components or the interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.

[0029] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Moreover, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0030] Reference Figures 1 to 3 The present invention provides a bioluminescence detector, comprising a base plate 1, a reagent rack 2 and a luminescence detection module 5 arranged on the base plate 1, wherein the reagent rack 2 is slidably connected to a reagent kit 3, a filter assembly 4 is arranged above the reagent kit 3, and the luminescence detection module 5 is arranged above the filter assembly 4;

[0031] The filter assembly 4 is provided with a plurality of filters 41 for selecting light waves, and the luminescence detection module 5 detects the light signal in the reagent kit 3 through the filters 41 .

[0032] In the above embodiment, the reagent rack 2 is provided with a groove that fits with the reagent kit 3 and can slide. A snap-on is provided at the outermost end so that the reagent kit 3 can be clamped with the reagent rack 2 after sliding into place. The reagent kit 3 has multiple independent reagent slots. The filter assembly 4 covers the top of the reagent kit 3. The filter 41 of the filter assembly 4 can be replaced according to the detection requirements to adapt to the detection of light signals of different wavelengths. The replacement of the filter 41 can be performed manually or designed as an automatic replacement mechanism. The control system automatically selects the appropriate filter 41 for detection, thereby improving detection efficiency and flexibility.

[0033] Luminescence detection module 5 is disposed above filter assembly 4 and is capable of detecting reagent kit 3 through filter 41. Luminescence detection module 5 can utilize a highly sensitive photodetector, preferably a photomultiplier tube or silicon photodiode, to ensure detection of weak light signals. Furthermore, luminescence detection module 5 can be equipped with a temperature control device to maintain temperature stability during detection, minimizing the impact of temperature fluctuations on test results.

[0034] In summary, by sliding the reagent kit 3 in the reagent rack 2 and arranging the filter assembly 4 and the luminescence detection module 5 above the reagent kit 3, the overall structure is compact and the reagent kit 3 and the filter 41 are easy and convenient to replace; by using the filter 41, the light signal of non-target wavelength can be removed, and the background light interference can be reduced, thereby improving the purity of the detection signal, so that the luminescence detection module 5 can detect the target wavelength light signal more specifically, and enhance the accuracy of the detection results. In addition, the filter assembly 4 includes multiple filters 41, which can select appropriate filter bands according to different types of chemical reactions to meet different detection requirements, thereby expanding the application range of the detector and making it suitable for a variety of bioluminescence reactions.

[0035] refer to Figure 1 、 Figure 2 and Figure 4 In one embodiment, a transport mechanism 6 is provided on the base plate 1, and the transport mechanism 6 includes a first guide rail 61, which is provided on the upper end surface of the base plate 1. The first guide rail 61 is slidably connected to a slider 62, and the slider 62 is fixedly connected to the reagent rack 2. The slider 62 is connected to the driving end of the driving assembly to drive the reagent rack 2 to slide along the first guide rail 61.

[0036] In the above embodiment, the slider 62 is fixedly connected to the reagent rack 2, ensuring that the reagent rack 2 remains stable during sliding. The slider 62 slides on the first guide rail 61, and the precise control of the drive assembly achieves smooth movement and positioning of the reagent rack 2, allowing for rapid replacement of reagent cartridges 3, improving the operating efficiency of the detector, and reducing manual errors.

[0037] In one embodiment, the driving assembly includes a first motor 63 fixedly connected to the base plate 1, the driving end of the first motor 63 is connected to a first synchronous wheel 64, and the end of the first guide rail 61 away from the first motor 63 is provided with a second synchronous wheel 65, and the outer sides of the first synchronous wheel 64 and the second synchronous wheel 65 are connected to a belt 66 for transmission, and the belt 66 is fixedly connected to the slider 62.

[0038] In the above embodiment, the first synchronous wheel 64 rotates with the rotation of the first motor 63, thereby driving the belt 66 to move. Since the belt 66 is fixedly connected to the slider 62, the slider 62 moves along the first guide rail 61. The second synchronous wheel 65 rotates synchronously with the first synchronous wheel 64 to ensure that the belt 66 moves smoothly and the slider 62 moves smoothly, which not only improves the accuracy of the movement of the reagent rack 2, but also reduces mechanical wear and extends the service life of the equipment. In addition, by accurately controlling the speed and rotation direction of the first motor 63, the accurate positioning of the slider 62 can be achieved, thereby quickly replacing the reagent box 3, further improving the working efficiency of the detector.

[0039] refer to Figure 1 In one embodiment, a shell 7 is provided on the upper end surface of the base plate 1, and a detection cavity for accommodating the reagent rack 2 is provided in the shell 7, and the filter assembly 4 and the luminescence detection module 5 are fixedly connected to the shell 7 respectively.

[0040] In the above embodiment, the interior of the detection chamber is conducive to the reasonable placement of the reagent rack 2, while ensuring the safety and accuracy of the reagents during the detection process. The detection chamber is preferably set to a completely dark environment, which is conducive to the accurate detection of the luminescence detection module 5. The filter assembly 4 and the luminescence detection module 5 are respectively fixedly connected to the housing 7, so that the filter assembly 4 can stably illuminate the reagents through a light beam of a specific wavelength, while the luminescence detection module 5 is responsible for receiving the signal after the filtered light processing.

[0041] refer to Figure 2 In one embodiment, a guide plate 8 is fixedly connected to the housing 7 , and the guide plate 8 is provided with a guide portion for the reagent rack 2 to slide in and out, so that the guide plate 8 is slidably connected to the reagent rack 2 .

[0042] In the above embodiment, the guide plate 8 is made of a wear-resistant material with good lubricity to reduce friction during the sliding of the reagent rack 2, thereby reducing wear and noise. The guide portion ensures the stability and accuracy of the reagent rack 2 during sliding, preventing the reagent rack 2 from getting stuck or deviating from the predetermined track due to vibration or tilt. The guide portion is slotted, and the reagent rack 2 is provided with a stopper protrusion corresponding to the slot.

[0043] refer to Figure 3 In one embodiment, the filter assembly 4 includes a second motor 42 and a filter wheel 43. The second motor 42 is arranged on the upper end surface of the housing 7. The filter wheel 43 is rotatably connected to the inner top wall of the housing 7. The output shaft of the second motor 42 penetrates the housing 7 and is connected to the filter wheel 43. A plurality of light-transmitting holes are opened in the axial direction of the filter wheel 43, and the filter 41 is arranged in the light-transmitting holes.

[0044] In the above embodiment, the inner top wall of the housing 7 is provided with a slot for the filter wheel 43 to be embedded and rotatable. The housing 7 is provided with a through hole to allow the output shaft of the second motor 42 to pass through and connect with the filter wheel 43. The second motor 42 is provided on the upper end surface of the housing 7.

[0045] The filter 41 is preferably made of quartz glass to ensure its transmittance and durability. The rotation of the filter wheel 43 is precisely controlled by a second motor 42, which rapidly switches between different filters 41 by controlling signals on the circuit board. Each filter 41 corresponds to a specific wavelength of light, enabling the luminescence detection module 5 to perform precise spectral analysis for different detection criteria. The design of the filter assembly 4 not only improves detection sensitivity and accuracy, but also reduces maintenance costs and failure rates through an optimized mechanical structure.

[0046] refer to Figure 5 In one embodiment, the housing 7 is open at one end away from the first motor 63, and a connecting plate 9 is provided at the opening of the housing 7. The connecting plate 9 is provided with an avoidance portion for the reagent rack 2 to slide in and out. The end surface of the connecting plate 9 away from the first motor 63 is provided with a second guide rail 10, and a sealing door 11 is slidably connected to the second guide rail 10.

[0047] In the above embodiment, connecting plate 9 is fixed to housing 7, and the opening of connecting plate 9 is connected to sealing door 11. The sliding connection of sealing door 11 uses a built-in sliding clamping portion to tightly mate with second guide rail 10, ensuring stability and sealing during operation. Sealing door 11 is made of corrosion-resistant stainless steel to accommodate the various chemicals in the laboratory environment.

[0048] When the test kit 3 needs to be replaced, the sealing door 11 is opened and the tester enters and exits through the avoidance part of the connecting plate 9. During the detection operation, the sealing door 11 is closed to ensure the closedness of the laboratory environment and prevent external air and pollutants from entering and affecting the accuracy of the test results. At the same time, the sealing door 11, connecting plate 9, shell 7 and guide plate 8 together form a completely black environment in the detection chamber, further ensuring the sensitivity and accuracy of the detection.

[0049] In one embodiment, a third motor 12 is provided on one side of the connecting plate 9, and the output shaft of the third motor 12 is connected to a gear 13. A rack 14 meshing with the gear 13 is provided on one side of the sealing door 11, so that the sealing door 11 slides along the second guide rail 10 through the drive of the third motor 12.

[0050] In the above embodiment, the third motor 12 achieves precise control of the sealing door 11 through the gear 13 and rack 14 mechanism, ensuring its stability and positioning accuracy during the sliding process.

[0051] In one embodiment, a pressure plate 15 is provided on the side of the slider 62 close to the belt 66, the bottom end of the pressure plate 15 abuts against the belt 66, and the bottom end of the pressure plate 15 is fixedly connected to a bite plate 16, and the bite plate 16 is bite-fixed with the belt 66 to fix the slider 62 to the belt 66.

[0052] In the above embodiment, the bite plate 16 and the belt 66 are fixed in an interlocking manner, so that the bite plate 16 is fixed to the bottom end of the belt 66, and through the fixed connection with the pressure plate 15, the stability and reliability of the slider 62 driven by the belt 66 during the movement are ensured. Furthermore, the bite plate 16 enables the slider 62 to maintain a tight connection with the belt 66 when subjected to external force, thereby avoiding the risk of sliding or falling off.

[0053] The above description is only a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the description and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A bioluminescence detector, characterized in that: The apparatus comprises a base plate (1), a reagent rack (2) and a luminescence detection module (5) arranged on the base plate (1), wherein the reagent rack (2) is slidably connected to a reagent box (3), a filter assembly (4) is arranged above the reagent box (3), and the luminescence detection module (5) is arranged above the filter assembly (4); The optical filter assembly (4) is provided with a plurality of optical filters (41) for selecting light waves, and the luminescence detection module (5) detects the light signal in the reagent kit (3) through the optical filters (41).

2. A bioluminescence detector according to claim 1, characterized in that: A transport mechanism (6) is provided on the base plate (1), and the transport mechanism (6) includes a first guide rail (61). The first guide rail (61) is provided on the upper end surface of the base plate (1). The first guide rail (61) is slidably connected to a slider (62). The slider (62) is fixedly connected to the reagent rack (2), and the slider (62) is connected to the driving end of the driving assembly to drive the reagent rack (2) to slide along the first guide rail (61).

3. A bioluminescence detector according to claim 2, characterized in that: The driving assembly comprises a first motor (63) fixedly connected to the base plate (1); a driving end of the first motor (63) is connected to a first synchronous wheel (64); an end of the first guide rail (61) away from the first motor (63) is provided with a second synchronous wheel (65); outer sides of the first synchronous wheel (64) and the second synchronous wheel (65) are connected to each other by a belt (66); and the belt (66) is fixedly connected to the slider (62).

4. A bioluminescence detector according to claim 3, characterized in that: The upper end surface of the base plate (1) is provided with a housing (7), and a detection cavity for accommodating the reagent rack (2) is provided in the housing (7). The filter assembly (4) and the luminescence detection module (5) are respectively fixedly connected to the housing (7).

5. A bioluminescence detector according to claim 4, characterized in that: A guide plate (8) is fixedly connected to the housing (7), and the guide plate (8) is provided with a guide portion for the reagent rack (2) to slide in and out, so that the guide plate (8) is slidably connected to the reagent rack (2).

6. A bioluminescence detector according to claim 4, characterized in that: The filter assembly (4) includes a second motor (42) and a filter wheel (43), wherein the second motor (42) is arranged on the upper end surface of the housing (7), and the filter wheel (43) is rotatably connected to the inner top wall of the housing (7), and the output shaft of the second motor (42) penetrates the housing (7) and is connected to the filter wheel (43), and a plurality of light-transmitting holes are opened in the axial direction of the filter wheel (43), and the filter (41) is arranged in the light-transmitting holes.

7. The bioluminescence detector according to claim 4, characterized in that: The housing (7) is open at one end away from the first motor (63), and a connecting plate (9) is provided at the opening of the housing (7). The connecting plate (9) is provided with an escape portion for the reagent rack (2) to slide in and out. A second guide rail (10) is provided at one end face of the connecting plate (9) away from the first motor (63), and a sealing door (11) is slidably connected to the second guide rail (10).

8. The bioluminescence detector according to claim 7, characterized in that: A third motor (12) is provided on one side of the connecting plate (9), an output shaft of the third motor (12) is connected to a gear (13), and a rack (14) meshing with the gear (13) is provided on one side of the sealing door (11), so that the sealing door (11) slides along the second guide rail (10) through the drive of the third motor (12).

9. The bioluminescence detector according to claim 3, characterized in that: A pressure plate (15) is provided on one side of the slider (62) close to the belt (66), the bottom end of the pressure plate (15) abuts against the belt (66), and the bottom end of the pressure plate (15) is fixedly connected to a bite plate (16), and the bite plate (16) is fixedly engaged with the belt (66) so that the slider (62) is fixedly connected to the belt (66).

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