Cell analyzer and detection method thereof

Abstract

The present invention is applicable to the field of medical equipment, and discloses a cell analyzer and a detection method for the cell analyzer. The cell analyzer includes a sampling component, an optical channel reaction pool, an optical detection unit, a hemoglobin detection unit, an impedance counting detection unit, a first reagent providing device, a second reagent providing device, a diluent providing device, a conveying pipeline assembly and a fluid power device. The fluid power device includes a first syringe and a second syringe. The range of the first syringe is smaller than the range of the second syringe. The second syringe is used to drive the optical detection sample from the optical channel reaction pool to the optical sample preparation pipeline. The first syringe is used to provide power for sample aspiration, sample separation and sample pushing. The second syringe is used to provide power for diluent delivery to the flow chamber, diluent delivery to the hemoglobin detection unit, diluent delivery to the impedance counting detection unit and sample preparation. The present invention greatly reduces the cost and volume of the instrument.

Description

Technical Field

[0001] The present invention relates to the field of medical equipment, and in particular to a cell analyzer and a detection method for the cell analyzer. Background Art

[0002] In order to drive the transportation of reagents and samples to achieve measurement, the five-differentiation blood cell analyzer generally includes a reagent transportation power source and a sampling and pushing power source.

[0003] Among them, the existing reagent delivery power sources mainly include the following design schemes:

[0004] 1) The power source for reagent delivery adopts an external gas source solution that can provide a large flow rate: This solution using an external gas source as the power source for reagent delivery can provide high pressure and large flow rate (generally providing a no-load flow rate greater than 10L / min and a pressure of more than 200kPa). It can drive components such as cylinders, pneumatic pressure-breaking valves, and pneumatic metering pumps, and can drive the filling and supply of liquid reservoirs, waste liquid collection and drainage, etc. Its driving components have a fast execution speed and can support high-gas-consuming components to work simultaneously (the average gas consumption of the whole machine is more than 5L / min). Therefore, this type of model has a fast measurement speed.

[0005] However, the external air source solution not only requires an independent air source outside the instrument, which significantly increases cost and size, but also requires internal conditioning components such as a dehumidifier, air filter, dryer, precision pressure regulator, and relief valve. This results in high instrument cost and a large size. Therefore, this solution is generally only used on high-end instruments with high measurement speed requirements.

[0006] 2) The power source for reagent delivery adopts a solution with a built-in small gas source (air pump): This solution of configuring a small gas source inside the machine as the power source for reagent delivery can provide power with higher pressure and larger flow (generally providing a no-load flow of more than 2L / min and a pressure of more than 120kPa), can drive small cylinders, pneumatic pressure-breaking valves, pneumatic metering pumps and other components to work, and can drive functions such as filling and supplying liquid to the liquid reservoir, collecting and draining waste liquid. Due to the limited flow of miniaturized gas pumps, it is generally necessary to add gas tanks to support the simultaneous operation of high-gas-consuming components (the average gas consumption of the whole machine is more than 1.5L / min). The cost of the air pump in this solution is still relatively high, and since a larger gas tank or pressure regulating element is required, the size of the instrument is still relatively large; therefore, this solution is generally used on mid-range machines with higher measurement speed requirements.

[0007] 3) The solution of using syringes as the power source for reagent delivery: In this solution, all reagent quantitative delivery uses syringes. When there are many types of reagents, the corresponding number of syringes will be large, and the cost of syringes is generally high and the volume is large. Therefore, this solution still cannot meet the needs in terms of low cost and miniaturization design.

[0008] Existing sampling and pushing power sources generally adopt a design scheme of multiple syringes.

[0009] In summary, existing designs of reagent delivery power sources and sampling and pushing power sources generally have the problems of high cost and large size, which makes it difficult to meet the design requirements of low cost and small size of instruments. Summary of the Invention

[0010] The first object of the present invention is to provide a cell analyzer, which aims to solve the technical problems of large size and high cost of the power source of the existing cell analyzer.

[0011] To achieve the above object, the present invention provides a solution: a cell analyzer comprising:

[0012] A sampling component, used for collecting samples to be tested;

[0013] An optical channel reaction pool, which is used to provide a reaction site for the sample to be tested and the reagent to prepare an optical detection sample;

[0014] an optical detection unit, the optical detection unit comprising a flow chamber and an optical detection element, the flow chamber having a diluent inlet, a sample inlet, a first outlet, and a detection zone for allowing the optical detection sample to pass through under the influence of the diluent, the diluent inlet, the sample inlet, and the first outlet being respectively connected to the detection zone, and the optical detection element being used to detect the optical detection sample carried by the diluent through the detection zone;

[0015] A hemoglobin detection unit, the hemoglobin detection unit is used to provide a reaction site for the test sample and the reagent to prepare a hemoglobin detection sample, and is used to detect the hemoglobin concentration of the hemoglobin detection sample;

[0016] An impedance counting detection sample preparation unit, the impedance counting detection sample preparation unit is used to provide a reaction field for the sample to be tested and the diluent to prepare an impedance counting detection sample;

[0017] An impedance counting detection unit, configured to perform impedance counting detection on an impedance counting detection sample;

[0018] a first reagent providing device, the first reagent providing device being used to provide reagents for the optical channel reaction cell;

[0019] a second reagent providing device, the second reagent providing device being used to provide reagents for the hemoglobin detection unit;

[0020] a diluent providing device, the diluent providing device being used to provide a diluent;

[0021] a delivery pipeline assembly, the delivery pipeline assembly comprising an optical sample preparation pipeline, a first diluent delivery pipeline, a second diluent delivery pipeline, and a third diluent delivery pipeline, the optical sample preparation pipeline being connected to the sample inlet and the optical channel reaction pool, respectively; the first diluent delivery pipeline being connected to the diluent inlet and the diluent supply device, respectively; the second diluent delivery pipeline being connected to the diluent supply device and the hemoglobin detection unit, respectively; and the third diluent delivery pipeline being connected to the diluent supply device and the impedance counting detection unit, respectively;

[0022] A fluid power device, comprising a first syringe and a second syringe, wherein the measuring range of the first syringe is smaller than the measuring range of the second syringe;

[0023] The first syringe is connected to the sampling component to drive the sampling component to absorb the sample to be tested, and is used to drive the sample to be tested in the sampling component to be respectively transported to the hemoglobin detection unit, the optical channel reaction pool, and the impedance counting detection sample preparation unit for reaction. The first syringe is also connected to the optical sample preparation pipeline to drive the optical detection sample from the optical sample preparation pipeline to be transported into the flow chamber;

[0024] The second syringe is connected to the first diluent delivery pipeline for driving the diluent from the diluent supply device to the flow chamber, the second syringe is also connected to the optical sample preparation pipeline for driving the optical detection sample from the optical channel reaction pool to the optical sample preparation pipeline, the second syringe is also connected to the second diluent delivery pipeline for driving the diluent from the diluent supply device to the hemoglobin detection unit, the second syringe is also connected to the impedance counting detection sample preparation unit for driving the diluent from the diluent supply device to the impedance counting detection sample preparation unit, and the second syringe is also connected to the third diluent delivery pipeline for driving the diluent from the diluent supply device to the impedance counting detection unit.

[0025] The second object of the present invention is to provide a detection method of a cell analyzer, which includes a sampling step, a diluent delivery step, a sample splitting step, an optical detection step, a hemoglobin detection step, and an impedance counting detection step.

[0026] The sampling step includes: driving the sampling component to absorb the sample to be tested by the first syringe;

[0027] The diluent delivery step includes: driving the diluent from the diluent supply device to deliver the diluent to the impedance counting detection sample preparation unit and the hemoglobin detection unit respectively through a second syringe, wherein the range of the second syringe is greater than the range of the first syringe;

[0028] The sample splitting step includes: driving the sample to be tested in the sampling component to be transported to the optical channel reaction pool, the hemoglobin detection unit and the impedance counting detection sample preparation unit respectively through the first syringe;

[0029] The optical detection step includes: providing a reagent to the optical channel reaction pool by a first reagent supply device to prepare an optical detection sample through the optical channel reaction pool; driving the optical detection sample from the optical channel reaction pool to the optical sample preparation pipeline by a second syringe; driving a diluent from the diluent supply device to the flow chamber by the second syringe, and simultaneously driving the optical detection sample to the flow chamber by the first syringe, so that the diluent carries the optical detection sample through the detection zone of the flow chamber; and detecting the optical detection sample carried by the diluent through the detection zone by the optical detection element.

[0030] The hemoglobin detection step includes: providing a reagent to the hemoglobin detection unit by a second reagent providing device to prepare a hemoglobin detection sample by the hemoglobin detection unit; and detecting the hemoglobin concentration of the hemoglobin detection sample by the hemoglobin detection unit;

[0031] The impedance counting detection step includes: preparing an impedance counting detection sample by the impedance counting detection sample preparation unit, and performing impedance counting detection on the impedance counting detection sample by the impedance counting detection unit.

[0032] The cell analyzer and detection method for the cell analyzer provided by the present invention reduce the number of power sources of the instrument by reusing the first syringe to provide power for sampling, sample splitting, and sample pushing in the optical detection channel, and reusing the second syringe to provide power for delivering the diluent to the flow chamber, delivering the diluent to the hemoglobin detection unit, delivering the diluent to the impedance counting detection unit, and preparing the optical sample, thereby significantly reducing the cost and size of the instrument and facilitating the miniaturization design of the cell analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0034] Figure 1 Schematic diagram of the fluid path system of the blood cell analyzer provided in Example 1 of the present invention;

[0035] Figure 2 This is a schematic diagram of the fluid connection between the optical channel reaction pool, sampling component, optical detection unit, diluent supply device and fluid power device provided in Example 1 of the present invention;

[0036] Figure 3 This is a schematic diagram of the fluid connection between the impedance counting detection unit, the hemoglobin detection unit, the diluent providing device, and the fluid power device provided in the first embodiment of the present invention;

[0037] Figure 4 Schematic diagram of the fluid path system of the blood cell analyzer provided in the second embodiment of the present invention;

[0038] Figure 5 Schematic diagram of the fluid path system of the blood cell analyzer provided in the third embodiment of the present invention;

[0039] Figure 6 Schematic diagram of the fluid path system of the blood cell analyzer provided in the fourth embodiment of the present invention;

[0040] Figure 7 Schematic diagram of the fluid path system of the blood cell analyzer provided in the fifth embodiment of the present invention;

[0041] Figure 8 Schematic diagram of the fluid path system of the blood cell analyzer provided in Example 6 of the present invention;

[0042] Figure 9 Schematic diagram of the fluid path system of the blood cell analyzer provided in Example 7 of the present invention.

[0043] Description of Figure Numbers:

[0044] 100, sampling component; 200, optical channel reaction pool; 210, first reaction pool; 220, second reaction pool; 300, optical detection unit; 310, flow chamber; d, diluent inlet; e, sample inlet; f, first outlet; 320, optical detection element; 400, hemoglobin detection unit; 500, impedance counting detection unit; g, second outlet; h, second inlet; i, first inlet; 510, impedance counting detection device; 520, diluent reservoir; 530, positive pressure power source; 540, pressure sensor; 550, fifth diluent delivery pipeline; 551, sixth control valve; 560, negative pressure power source; 570, air valve; 600, first reagent Providing device; 610, first metering pump; 620, second metering pump; 630, third metering pump; 640, fourth metering pump; 700, second reagent providing device; 710, fifth metering pump; 800, delivery pipeline assembly; 810, optical sample preparation pipeline; 811, first sample preparation pipeline; 812, second sample preparation pipeline; 820, sampling delivery pipe; 830, first connecting pipeline; 831, first controllable valve; 840, second connecting pipeline; 841, second controllable valve; 850, third connecting pipeline; 851, third controllable valve; 860, fourth connecting pipeline; 861, fourth controllable valve; 862, fifth controllable valve; 870, fifth connecting pipeline; 880, first connector; a, first interface; b, second interface; c, third interface; 890, second connector; 8100, third connector; 8110, fourth connector; 8120, first diluent delivery pipeline; 8121, first control valve; 8122, second control valve; 8130, sixth connector; 8140, third diluent delivery pipeline; 8141, fourth control valve; 8150, fourth diluent delivery pipeline; 8151, fifth control valve; 8160, first delivery pipeline; 8161, seventh control valve; 8170, impedance counting sample preparation pipeline; 8180, second delivery pipeline; 8181, eighth control valve; 8190, seventh connector; 820 0. Second diluent delivery pipeline; 8201. Third control valve; 8210. Third delivery pipeline; 8211. Ninth control valve; 8220. First discharge pipeline; 8221. First discharge control valve; 8230. Second discharge pipeline; 8231. Second discharge control valve; 8240. Third discharge pipeline; 8241. Third discharge control valve; 8250. Fourth discharge pipeline; 8251. Fourth discharge control valve; 8260. Fifth discharge pipeline; 8261. Fifth discharge control valve; 8270. Fifth connector; 900. Fluid power device; 910. First syringe; 920. Second syringe; 1000. Waste liquid tank; 1100. Diluent providing device.

[0045] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0046] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0047] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship and movement status of the various components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0048] It should also be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or indirectly connected to the other element through an intervening element.

[0049] In addition, the descriptions of "first", "second", etc. in the present invention are for descriptive purposes only and should not be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but this must be based on the fact that they can be implemented by ordinary technicians in this field. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0050] Example 1:

[0051] like Figure 1-3 As shown, the blood cell analyzer provided in the first embodiment of the present invention includes a sampling component 100, an optical channel reaction pool 200, an optical detection unit 300, a hemoglobin detection unit 400, an impedance counting detection unit 500, a first reagent providing device 600, a second reagent providing device 700, a delivery pipeline assembly 800, a fluid dynamic device 900, a waste liquid tank 1000 and a diluent providing device 1100. The sampling component 100, the optical channel reaction pool 200, the optical detection unit 300, the hemoglobin detection unit 400, the impedance counting detection unit 500, the first reagent providing device 600, the second reagent providing device 700, the waste liquid tank 1000, the diluent providing device 1100 and the fluid dynamic device 900 are connected through the delivery pipeline assembly 800 to form a liquid system.

[0052] In this embodiment, the fluid power device 900 includes a first syringe 910 and a second syringe 920, and the range of the first syringe 900 is smaller than the range of the second syringe 920. Among them, the first syringe 910 is reused to provide power for sampling, blood separation (sample separation), and sample pushing (including optical channel sample pushing and sheath flow impedance sample pushing). The second syringe 920 is reused to provide power for sample dilution, sample flow component cleaning, sample preparation, impedance counting detection unit 500 filling, and optical detection sheath fluid pushing. In this embodiment, the fluid power device 900 has only two syringes that are highly reused, which can achieve the purpose of reducing the cost and volume of the instrument.

[0053] Preferably, the first syringe 910 and the second syringe 920 are driven by independent power mechanisms. Specifically, the fluid power device 900 further includes a first motor (not shown) for driving the first syringe 910 and a second motor (not shown) for driving the second syringe 920. The first motor and the second motor are two independently configured motors. The step length of the second motor is preferably greater than the step length of the first motor.

[0054] Preferably, the measuring range of the first syringe 910 is microliter-scale. More preferably, the measuring range of the first syringe 910 is between 100uL and 300uL. As a preferred implementation scheme of this embodiment, the measuring range of the first syringe 910 is 250uL. In this preferred implementation scheme, the measuring range of the first syringe 910 is relatively large. On the one hand, it is beneficial to improve the quantitative accuracy of the first syringe 910. On the other hand, it is beneficial for the first syringe 910 to select a motor with a smaller step as the driving motor. For example, the first syringe 910 of 250uL here can select a motor with a step of half the step of the motor used for the 100uL syringe as the driving motor. In this way, the requirements of quantitative accuracy and quantitative volume can be taken into account at the same time. At the same time, the motor can run at high speed to meet the demand for high-speed suction and discharge flow of the first syringe 910 during the erythrocyte sedimentation rate test.

[0055] Preferably, the volume range of the second syringe 920 is in the milliliter range. As a preferred embodiment of this embodiment, the volume range of the second syringe 920 is 10 mL.

[0056] The optical detection unit 300 includes a flow chamber 310 and an optical detection element 320. The flow chamber 310 has a detection zone, a diluent inlet d, a sample inlet e, and a first outlet f. The diluent inlet d, the sample inlet e, and the first outlet f are each connected to the detection zone. The detection zone is used for passing the sample under the diluent for optical detection. The optical detection element 320 may include a light source, a forward scattered light signal collection device arranged on the optical axis, a side scattered light signal collection device arranged to the side of the optical axis, and a fluorescence signal collection device.

[0057] The optical channel reaction pool 200 is used to provide a reaction site for the test sample and the reagent to prepare the optical detection sample. In this embodiment, the optical detection sample includes a reticulocyte detection sample and a white blood cell differential detection sample. The reticulocyte detection sample can be prepared by reacting the test sample with the red blood cell reagent, and the white blood cell differential detection sample can be prepared by reacting the test sample with the white blood cell reagent. Of course, the type of optical detection sample is not limited to this. In specific applications, it can be set according to the actual requirements of the detection project. For example, the optical detection sample can also include only one of the reticulocyte detection sample and the white blood cell differential detection sample; or, the optical detection sample can also include other sample liquids other than the reticulocyte detection sample and the white blood cell differential detection sample. In specific applications, it can be designed accordingly according to the actual requirements of the detection project.

[0058] In this embodiment, the optical channel reaction pool 200 includes a first reaction pool 210 and a second reaction pool 220. The first reaction pool 210 and the second reaction pool 220 are two independent reaction pools, that is, the number of optical channel reaction pools 200 is two. Among them, the first reaction pool 210 is used to provide a reaction site for the sample to be tested and the red blood cell reagent to prepare a reticulocyte detection sample. The second reaction pool 220 is used to provide a reaction site for the sample to be tested and the white blood cell reagent to prepare a white blood cell differential detection sample. In this embodiment, the reticulocyte detection sample and the white blood cell differential detection sample are prepared in two independent reaction pools, respectively. On the one hand, it can avoid the problem of cross-contamination caused by reagent residue when sharing a reaction pool; on the other hand, it can enable the white blood cell differential detection sample and the reticulocyte detection sample to be prepared at the same time, which is conducive to improving detection efficiency.

[0059] The transport pipeline assembly 800 includes an optical sample preparation pipeline 810, a sampling transport pipeline 820, a first connecting pipeline 830, a second connecting pipeline 840, a third connecting pipeline 850, a fourth connecting pipeline 860, a fifth connecting pipeline 870, a first connector 880, a second connector 890, a third connector 8100, a fourth connector 8110, a first diluent transport pipeline 8120, a third diluent transport pipeline 8140, a fourth diluent transport pipeline 8150, a first transport pipeline 8160, an impedance counting sample preparation pipeline 8170, a second transport pipeline 8180 and a seventh connector 8190, a second diluent transport pipeline 8200, a third transport pipeline 8210, a first drain pipeline 8220, a second drain pipeline 8230, a third drain pipeline 8240, a fourth drain pipeline 8250 and a fifth drain pipeline 8260. Among them, the optical sample preparation pipeline 810 is respectively connected to the sample inlet e, the optical channel reaction pool 200, the first syringe 910, and the second syringe 920; the first diluent delivery pipeline 8120 is respectively connected to the diluent inlet d and the diluent providing device 1100; the second diluent delivery pipeline 8200 is respectively connected to the diluent providing device 1100, the hemoglobin detection unit 400, and the second syringe 920; the third diluent delivery pipeline 8140 is respectively connected to the diluent providing device 1100, the impedance counting detection unit 500, and the second syringe 920.

[0060] The diluent supply device 1100 is used to provide a diluent. The second syringe 920 is connected to the diluent supply device 1100 and the diluent inlet d via a first diluent delivery line 8120, respectively, for driving the diluent from the diluent supply device 1100 to the flow chamber 310. The second syringe 920 is connected to the optical sample preparation line 810, for driving the optical detection sample from the optical channel reaction pool 200 to the optical sample preparation line 810. The second syringe 920 is also connected to the diluent supply device 1100 and the hemoglobin detection unit 400 via a second diluent delivery line 8200, respectively, for driving the diluent from the diluent supply device 1100 to the hemoglobin detection unit 400. The second syringe 920 is also connected to the diluent supply device 1100 and the impedance counting detection unit 500 via a third diluent delivery line 8140, respectively, for driving the diluent from the diluent supply device 1100 to the impedance counting detection unit 500.

[0061] The sampling component 100 is used to collect the sample to be tested. In this embodiment, the sample to be tested is a blood sample. Of course, as an alternative embodiment, the sample to be tested can also be a body fluid sample. The sampling delivery tube 820 is connected between the first syringe 910 and the sampling component 100. The first syringe 910 is connected to the sampling component 100 to drive the sampling component 100 to absorb the sample to be tested (i.e., sampling) and drive the sample to be tested in the sampling component 100 to be transported to the hemoglobin detection unit 400 and the optical channel reaction pool 200 respectively (i.e., blood separation) for reaction. The sampling component 100 can be a sampling needle or a sampling pipette, etc. The blood cell analyzer also includes a power element (not shown) for driving the sampling component 100 to move. The sampling component 100 can be driven by the power element to move to a sample storage container (such as a test tube, etc.) for sampling, and then moved to the optical channel reaction pool 200 and the hemoglobin detection unit 400 respectively under the drive of the power element, and blood separation is performed under the drive of the first syringe 910.

[0062] The first syringe 910 is connected to the sample inlet e through the optical sample preparation pipeline 810 , so as to drive the optical detection sample to be transported from the optical sample preparation pipeline 810 into the flow chamber 310 .

[0063] During reticulocyte detection, the second syringe 920 is used to drive the reticulocyte detection sample from the first reaction pool 210 to the optical sample preparation pipeline 810 and drive the diluent to be delivered to the flow chamber 310; the first syringe 910 is used to drive the reticulocyte detection sample from the optical sample preparation pipeline 810 to be delivered to the flow chamber 310; the optical detection element 320 is used to perform reticulocyte detection on the reticulocyte detection sample carried by the diluent through the detection area, and obtain the reticulocyte detection result based on the scattered light signal and the fluorescence signal.

[0064] During white blood cell differentiation detection, the first syringe 910 is used to drive the white blood cell differentiation detection sample from the optical sample preparation pipeline 810 to the flow chamber 310 and to drive the diluent to be delivered into the flow chamber 310, and the second syringe 920 is used to drive the white blood cell differentiation detection sample from the second reaction pool 200 to the optical sample preparation pipeline 810; the optical detection element 320 is used to perform white blood cell differentiation detection on the white blood cell differentiation detection sample carried by the sheath fluid through the detection area.

[0065] The first reagent supply device 600 is used to supply reagents to the optical channel reaction cell 200. In this embodiment, the first reagent supply device 600 includes a first metering pump 610, a second metering pump 620, a third metering pump 630 and a fourth metering pump 640.

[0066] The first metering pump 610 is connected to the first reaction pool 210 for supplying a first reagent to the first reaction pool 210. The second metering pump 620 is connected to the first reaction pool 210 for supplying a second reagent to the first reaction pool 210. As a preferred embodiment of this embodiment, one of the first reagent and the second reagent is a fluorescent reagent and the other is a diluting reagent. The fluorescent reagent is used to enable the optical detection unit 300 to obtain a fluorescent signal during reticulocyte detection, and the diluting reagent is mainly used to dilute the sample to be tested. Of course, in specific applications, as an alternative embodiment, the fluorescent reagent and the diluting reagent can also be replaced by a reagent having both fluorescent and diluting effects. In this alternative embodiment, there is only one metering pump connected to the first reaction pool 210, for example, the first metering pump 610.

[0067] The third metering pump 630 is connected to the second reaction tank 220 for providing the third reagent to the second reaction tank 220; the fourth metering pump 640 is connected to the second reaction tank 220 for providing the fourth reagent to the second reaction tank 220. One of the third reagent and the fourth reagent is a hemolytic agent that can dissolve red blood cells in the sample to be tested and can distinguish different types of white blood cells, and the other is a reagent that can stain white blood cells. As a preferred embodiment of this embodiment, one of the third reagent and the fourth reagent is a hemolytic agent and the other is a fluorescent staining reagent; of course, in a specific application, as an alternative embodiment, the hemolytic agent and the fluorescent staining reagent can also be replaced by a reagent that has both the red blood cell dissolving function and the fluorescent staining function. In this alternative embodiment, there is only one metering pump connected to the second reaction tank 220, such as the third metering pump 630; alternatively, the fluorescent staining reagent can also be replaced by a chemical staining reagent.

[0068] The hemoglobin detection unit 400 is used to provide a reaction site for the sample to be tested and the fifth reagent to prepare the hemoglobin detection sample, provide a reaction site for the sample to be tested and the diluent to prepare the impedance counting detection sample, and perform hemoglobin concentration detection on the hemoglobin detection sample. In this embodiment, in addition to being used as a preparation unit and detection unit for the hemoglobin detection sample, the hemoglobin detection unit 400 is also used as an impedance counting detection sample preparation unit, that is, the impedance counting detection sample preparation unit and the hemoglobin detection unit 400 are integrated. During specific operation, the sample to be tested and the diluent can be added to the hemoglobin detection unit 400 for reaction to prepare the impedance counting detection sample, and then part of the impedance counting detection sample can be withdrawn; and then the fifth reagent can be added to the hemoglobin detection unit 400 to prepare the hemoglobin detection sample.

[0069] The second reagent supply device 700 is used to provide a fifth reagent to the hemoglobin detection unit 400 and includes a fifth metering pump 710 connected to the hemoglobin detection unit 400. The fifth reagent is, for example, a hemolytic agent that can dissolve red blood cells in a blood sample, release hemoglobin in the red blood cells, and convert hemoglobin into methemoglobin. In an alternative embodiment, the reagent used in the white blood cell differential test and the reagent used in the hemoglobin test can also be the same hemolytic agent, that is, the metering pump used to add reagent to the second reaction pool 220 and the metering pump used to add reagent to the hemoglobin detection unit 400 are the same component.

[0070] The optical sample preparation line 810 is disposed between the flow chamber 310 and the optical channel reaction well 200. The second syringe 920 is connected to the optical sample preparation line 810 to drive the optical detection sample from the optical channel reaction well 200 into the optical sample preparation line 810. The first syringe 910 is connected to the sample inlet e through the optical sample preparation line 810 to drive the optical detection sample from the optical sample preparation line 810 into the flow chamber 310.

[0071] In this embodiment, the optical sample preparation circuit 810 includes a first sample preparation circuit 811 and a second sample preparation circuit 812. Specifically, there are two optical sample preparation circuits 810, and the first sample preparation circuit 811 and the second sample preparation circuit 812 are independent circuits. The first sample preparation circuit 811 is connected to the first reaction cell 210, the flow chamber 310, the first syringe 910, and the second syringe 920, respectively. The second sample preparation circuit 812 is connected to the second reaction cell 220, the flow chamber 310, the second reaction cell 220, the first syringe 910, and the second syringe 920, respectively. During reticulocyte testing, the second syringe 920 is used to drive the reticulocyte test sample from the first reaction cell 210 into the first sample preparation circuit 811 and to drive the sheath fluid into the flow chamber 310. The first syringe 910 is used to drive the reticulocyte test sample from the first sample preparation circuit 811 into the flow chamber 310. During the white blood cell differential test, the second syringe 920 drives the white blood cell differential test sample from the second reaction reservoir 220 into the second sample preparation line 812 and drives the sheath fluid into the flow chamber 310. The first syringe 910 drives the white blood cell differential test sample from the second sample preparation line 812 into the flow chamber 310. In this embodiment, the reticulocyte test and the white blood cell differential test utilize separate reaction reservoirs and separate sample preparation lines, respectively, to minimize cross contamination. Of course, in specific applications, the number of reaction pools included in the optical channel reaction pool 200 is not limited to two. When there are at least two optical channel reaction pools 200, the preferred design scheme is: one optical channel reaction pool 200 is solely used to provide a reaction field for the sample to be tested and the red blood cell reagent to prepare the reticulocyte detection sample. The number of optical sample preparation pipelines 810 is at least two, and all optical sample preparation pipelines 810 are connected to the sample inlet e of the flow chamber 310, the optical channel reaction pool 200, the first syringe 910, and the second syringe 920. One of the optical sample preparation pipelines 810 is connected to the reaction pool for preparing the reticulocyte detection sample and is solely used for the reticulocyte detection sample and the diluent to flow through. This helps to reduce the impact of cross-contamination of the sample liquid on the detection accuracy.

[0072] One end of the first sample preparation line 811 is connected to the flow chamber 310, and the other end is connected to the first reaction pool 210 through the first connecting line 830. One end of the second sample preparation line 812 is connected to the flow chamber 310, and the other end is connected to the second reaction pool 220 through the third connecting line 850. The second syringe 920 is connected to the first sample preparation line 811 and the second sample preparation line 812 respectively through the second connecting line 840. The first syringe 910 is connected to the first sample preparation line 811 through the fourth connecting line 860 and to the second sample preparation line 812 through the fifth connecting line 870.

[0073] The first connecting line 830 is provided with a first controllable valve 831 , the second connecting line 840 is provided with a second controllable valve 841 , and the third connecting line 850 is provided with a third controllable valve 851 . When the first control valve 8121, the second controllable valve 841 and the first controllable valve 831 are opened, the second syringe 920 can drive the diluent to be delivered to the first reaction tank 210; when the first control valve 8121 is closed, and the second controllable valve 841 and the first controllable valve 831 are opened, the second syringe 920 can drive the reticulocyte detection sample from the first reaction tank 210 to be delivered to the first sample preparation pipeline 811; when the first control valve 8121, the second controllable valve 841 and the third controllable valve 851 are opened, the second syringe 920 can drive the diluent to be delivered to the second reaction tank 220; when the first control valve 8121 is closed, and the second controllable valve 841 and the third controllable valve 851 are opened, the second syringe 920 can drive the white blood cell classification detection sample from the second reaction tank 220 to be delivered to the second sample preparation pipeline 812.

[0074] One end of the fourth connecting line 860 is connected to the first syringe 910, and the other end is connected to the first sample preparation line 811. The fourth connecting line 860 is provided with a sixth control valve 861. One end of the fifth connecting line 870 is connected to the sixth control valve 861, and the other end is connected to the second sample preparation line 812. The sixth control valve 861 can control the first syringe 910 to switch between the first sample preparation line 811 and the second sample preparation line 812. Of course, as an alternative embodiment, the fourth connecting line 860 and the fifth connecting line 870 can also be connected to the first syringe 910 via two independent control valves.

[0075] Preferably, in this embodiment, the fourth connecting line 860 is further provided with a fifth controllable valve 862, which is located between the first syringe 910 and the fourth controllable valve 861. The sampling component 100 is connected to the first syringe 910 via the fifth controllable valve 862. One end of the sampling delivery tube 820 is connected to the sampling component 100, and the other end is connected to the fifth controllable valve 862. The fifth controllable valve 862 can control the switching of the first syringe 910 between the sampling component 100 and the sample preparation line. Of course, as an alternative embodiment, the sample preparation line and the sampling component 100 can also be connected to the first syringe 910 via two independent controllable valves.

[0076] The first connector 880 has a first port a, a second port b, and a third port c that are interconnected. The first port a is connected to the sample inlet e of the flow chamber 310, and the third port c is connected to the second syringe 920 via a second connecting line 840. One of the first sample preparation line 811 and the second sample preparation line 812 is connected between the second port b and the optical channel reaction cell 200. The other of the first sample preparation line 811 and the second sample preparation line 812 has one end connected to the optical channel reaction cell 200 and the other end connected to the second syringe 920 and the third port c, respectively. The fourth connecting line 860 connects the first sample preparation line 811 and the first connecting line 830 via a third connector 8100. The fifth connecting line 870 connects the second sample preparation line 812 and the third connecting line 850 via a fourth connector 8110. The third connector 8100 and the fourth connector 8110 are preferably both three-way connectors, that is, the first syringe 910 is connected to two optical sample preparation lines 810 via two three-way connectors, respectively.

[0077] In this embodiment, the second connecting line 840 further connects to the second syringe 920 and the diluent supply device 1100 via the first diluent delivery line 8120. Specifically, one end of the second connecting line 840 is connected to the first sample preparation line 811 and the second sample preparation line 812, respectively, while the other end of the second connecting line 840 is connected to the first diluent delivery line 8120. The second syringe 920 is also used to drive the diluent from the diluent supply device 1100 to the optical sample preparation line 810 and the optical channel reaction cell 200. Thus, the second syringe 920 can provide power for cleaning the first sample preparation line 811, the second sample preparation line 812, the first reaction cell 210, and the second reaction cell 220 with the diluent.

[0078] One end of the first diluent delivery line 8120 is connected to the diluent inlet d of the flow chamber 310, and the other end is connected to the second syringe 920. The diluent supply device 1100 and the second connecting line 840 are respectively connected to the first diluent delivery line 8120. The first diluent delivery line 8120 is provided with a first control valve 8121 and a second control valve 8122. The first control valve 8121 is located near the second syringe 920, and the second control valve 8122 is located near the diluent inlet d. The diluent supply device 1100 is connected to the first control valve 8121. The first control valve 8121 and the second control valve 8122 can control the opening and closing of the first diluent delivery line 8120. In a specific application, when the first control valve 8121 and the second control valve 8122 are open, the second syringe 920 can push the sheath liquid into the flow chamber 310. When the first control valve 8121 and the second control valve 8122 are closed, the second syringe 920 cannot push the sheath liquid into the flow chamber 310.

[0079] The second syringe 920 is connected to the diluent supply device 1100 and the first syringe 910 via a fourth diluent delivery line 8150, respectively, to drive the diluent from the diluent supply device 1100 to the first syringe 910. Specifically, the fourth diluent delivery line 8150 is connected between the first diluent delivery line 8120 and the first syringe 910. A fifth control valve 8151 is provided on the fourth diluent delivery line 8150. When the first control valve 8121 and the fifth control valve 8151 are opened, the second syringe 920 can drive the diluent to the first syringe 910, thereby facilitating the cleaning of the first syringe 910, the sampling component 100, and the sampling delivery tube 820.

[0080] The impedance counting detection unit 500 is used to perform impedance counting detection on the impedance counting detection sample, and the impedance counting detection may include red blood cell count detection and / or platelet count detection. The impedance counting detection sample can be prepared by reacting the sample to be tested with a diluent.

[0081] The impedance counting detection unit 500 includes an impedance counting detection device 510, a diluent storage tank 520, a positive pressure power source 530, a pressure sensor 540 and a fifth diluent delivery pipeline 550. The fifth diluent delivery pipeline 550 is provided with a sixth control valve 551. The positive pressure power source 530 and the pressure sensor 540 are respectively connected to the diluent storage tank 520, wherein the positive pressure power source 530 is connected to the diluent storage tank 520 through an air valve 570. The diluent reservoir 520 is connected to the impedance counting detection device 510 through the fifth diluent delivery pipeline 550, and is connected to the second syringe 920 and the diluent providing device 1100 through the third diluent delivery pipeline 8140; one end of the impedance counting sample preparation pipeline 8170 is connected to the impedance counting detection device 510, and is connected to the second syringe 920 through the first delivery pipeline 8160, and the other end of the impedance counting sample preparation pipeline 8170 is connected to the hemoglobin detection unit 400 through the second delivery pipeline 8180, and is connected to the first syringe 910 through the third delivery pipeline 8210; the second syringe The injector 920 is also used to drive the diluent from the diluent providing device 1100 to the diluent reservoir 520 and to drive the impedance counting detection sample from the hemoglobin detection unit 400 to the impedance counting sample preparation pipeline 8170. The positive pressure power source 530 is used to drive the diluent from the diluent reservoir 520 to the impedance counting detection device 520, so that the impedance counting detection sample flows in the impedance counting detection device 520 under the influence of the diluent; the first syringe 910 is also used to drive the impedance counting detection sample from the impedance counting sample preparation pipeline 8170 to the impedance counting detection device 510.

[0082] Specifically, one end of the third diluent delivery line 8140 is connected to the first diluent delivery line 8120, and the other end is connected to the diluent reservoir 520. One end of the first delivery line 8160 is connected to the third diluent delivery line 8140, and the other end is connected to the impedance counting sample preparation line 8170 and the impedance counting detection device 510, respectively. A seventh control valve 8161 is provided on the first delivery line 8160, a fourth control valve 8141 is provided on the third diluent delivery line 8140, and a sixth control valve 551 is provided on the fifth diluent delivery line 550. When the first and fourth control valves 8121 and 8141 are opened, the second syringe 920 can drive the diluent into the diluent reservoir 520 to fill the diluent reservoir. When the air valve 570 and the sixth control valve 551 are opened, the positive pressure power source 530 can drive the sheath fluid from the diluent reservoir 520 into the impedance counting detection device 510. In this embodiment, the third diluent delivery pipeline 8140 is indirectly connected to the diluent providing device 1100 and the second syringe 920 through the first diluent delivery pipeline 8120; of course, in a specific application, as an alternative implementation scheme, the third diluent delivery pipeline 8140 can also be changed to a scheme of directly connecting the diluent providing device 1100 and the second syringe 920.

[0083] One end of the impedance counting sample preparation line 8170 is connected to the impedance counting detection device 510 and the first delivery line 8160 via a seventh connector 8190. The other end of the impedance counting sample preparation line 8170 is connected to the second delivery line 8180 and the third delivery line 8210, respectively. The second delivery line 8180 is connected between the impedance counting sample preparation line 8170 and the hemoglobin detection unit 400. An eighth control valve 8181 is provided on the second delivery line 8180. When the seventh control valve 8161 and the eighth control valve 8181 are opened, the second syringe 920 can drive the impedance counting detection sample from the hemoglobin detection unit 400 into the impedance counting sample preparation line 8170.

[0084] The impedance counting detection device 510 comprises a front cell (not shown), a rear cell (not shown), a jewel hole (not shown), a second inlet h, a first inlet i, and a second outlet g. The second inlet h and the first inlet i are respectively connected to the front cell; the jewel hole is used to connect the front cell and the rear cell; and the second outlet g is connected to the rear cell. The second inlet h is connected to the fifth diluent delivery line 550. The first inlet i is connected to the first delivery line 8160 and the impedance counting sample preparation line 8170 via a seventh connector 8190. The second outlet g is connected to the waste liquid reservoir 1000.

[0085] The second diluent delivery line 8200 is connected between the first diluent delivery line 8120 and the hemoglobin detection unit 400. The impedance counting sample preparation line 8170 is connected to one end of the third delivery line 8210 and one end of the second delivery line 8180, respectively. The other end of the third delivery line 8210 is connected to the first syringe 910, and the other end of the second delivery line 8180 is connected to the second diluent delivery line 8200. A third control valve 8201 is provided on the second diluent delivery line 8200. The fifth metering pump 710 is connected to the second diluent delivery line 8200, and the connection points of the fifth metering pump 710 and the second delivery line 8180 on the second diluent delivery line 8200 are both located between the third control valve 8201 and the hemoglobin detection unit 400. When the first control valve 8121 and the third control valve 8201 are opened, the second syringe 920 can deliver the diluent to the hemoglobin detection unit 400. In this embodiment, the second diluent delivery pipeline 8200 is indirectly connected to the diluent providing device 1100 and the second syringe 920 through the first diluent delivery pipeline 8120; of course, in a specific application, as an alternative implementation scheme, the second diluent delivery pipeline 8200 can also be changed to a scheme of directly connecting the diluent providing device 1100 and the second syringe 920.

[0086] A ninth control valve 8211 is provided on the third delivery line 8210, and a fifth controllable valve 862 is located between the ninth control valve 8211 and the first syringe 910. The fourth connecting line 860 is connected to the ninth control valve 8211. The fifth controllable valve 862 can control the opening and closing of the channel between the first syringe 910 and the sampling delivery tube 820, as well as the opening and closing of the channel between the first syringe 910 and the ninth control valve 8211. That is, by regulating the fifth controllable valve 862, the state in which the first syringe 910 is connected to the sampling delivery tube 820 and the state in which the first syringe 910 is connected to the sample preparation line (including the optical sample preparation line 810 and the impedance counting sample preparation line 8170) can be switched. Through the cooperation of the ninth control valve 8211 and the fifth controllable valve 862, the opening and closing of the channel between the first syringe 910 and the impedance counting sample preparation pipeline 8170 can be controlled; through the cooperation of the sixth control valve 861, the ninth control valve 8211 and the fifth controllable valve 862, the opening and closing of the channel between the first syringe 910 and the first sample preparation pipeline 811 and the second sample preparation pipeline 812 can be controlled.

[0087] In this embodiment, the first syringe 910 is connected to the impedance counting sample preparation line 8170 and the optical sample preparation line 810 respectively through the ninth control valve 8211. Of course, in a specific application, as an alternative embodiment, the optical sample preparation line 810 and the impedance counting sample preparation line 8170 can also be connected to the first syringe 910 through independent control valves.

[0088] The first drainage line 8220 is connected between the first outlet f of the flow chamber 310 and the waste liquid pool 1000. A first drainage control valve 8221 is provided on the first drainage line 8220. During reticulocyte testing and white blood cell differential testing, the first drainage control valve 8221 is opened to transport the reticulocyte test sample and diluent, or the white blood cell differential test sample and diluent, flowing out of the flow chamber 310 through the testing area, to the waste liquid pool 1000.

[0089] The second drain line 8230 is connected between the first reaction tank 210 and the waste liquid tank 1000. The second drain line 8230 is provided with a second drain control valve 8231. The second drain control valve 8231 is opened to drain the first reaction tank 210 so that the cleaning waste liquid can be discharged into the waste liquid tank 1000 when the first reaction tank 210 is cleaned.

[0090] The third drain line 8240 is connected between the second reaction tank 220 and the waste liquid tank 1000. A third drain control valve 8241 is provided on the third drain line 8240. Opening the third drain control valve 8241 drains the second reaction tank 220 so that when cleaning the second reaction tank 220, the cleaning waste liquid can be discharged into the waste liquid tank 1000.

[0091] A fourth drain line 8250 is connected between the hemoglobin detection unit 400 and the waste liquid reservoir 1000. A fourth drain control valve 8251 is provided on the fourth drain line 8250. Opening the fourth drain control valve 8251 drains the hemoglobin detection unit 400, allowing the waste liquid to be discharged into the waste liquid reservoir 1000 during cleaning of the hemoglobin detection unit 400.

[0092] The fifth drainage line 8260 is connected between the second outlet g of the impedance counting detection device 510 and the waste liquid pool 1000. During impedance counting detection, the impedance counting detection sample and sheath fluid flowing out of the second outlet g of the impedance counting detection device 510 can be discharged into the waste liquid pool 1000.

[0093] It should be noted that there can be only one waste liquid tank 1000, that is, the first drain line 8220, the second drain line 8230, the third drain line 8240, the fourth drain line 8250 and the fifth drain line 8260 are connected to the same waste liquid tank 1000; of course, as an alternative embodiment, there can be more than two waste liquid tanks 1000, and at least two of the first drain line 8220, the second drain line 8230, the third drain line 8240, the fourth drain line 8250 and the fifth drain line 8260 are respectively connected to different waste liquid tanks 1000.

[0094] This embodiment can significantly reduce the cost and size of the instrument by time-sharing and multiplexing two syringes, which is conducive to achieving a low-cost and miniaturized design of the blood cell analyzer. Specifically, the fluid power device 900 has only two syringes, and the two syringes are driven separately. A microliter-level syringe (i.e., the first syringe 910) is used to provide power for sampling, blood separation, and sample pushing; a milliliter-level syringe (i.e., the second syringe 920) is used for other functions, mainly including providing power for sample dilution, cleaning of the reaction pool / sampling channel / sample preparation, filling of the diluent reservoir 520, optical detection of sheath fluid, and other all aspects involving the transportation of diluents and sample preparation.

[0095] Specifically, the first syringe 910 is used to provide power for sampling: the first syringe 910 drives the sampling component 100 to draw the sample to be tested from a sample storage container such as a test tube.

[0096] The first syringe 910 is used to provide power for blood separation. Specifically, the first syringe 910 drives the sample to be tested from the sampling component 100 to be transported to the first reaction pool 210, the second reaction pool 220 and the hemoglobin detection unit 400 respectively.

[0097] The first syringe 910 is used to provide power for pushing the sample. Specifically, during reticulocyte detection, the first syringe 910 is used to drive the reticulocyte detection sample from the first sample preparation pipeline 811 to be transported into the flow chamber 310; during white blood cell classification detection, the first syringe 910 is used to drive the white blood cell classification detection sample from the second sample preparation pipeline 812 to be transported into the flow chamber 310; during impedance counting detection, the first syringe 910 is used to drive the impedance counting detection sample from the impedance counting sample preparation pipeline 8170 to be transported into the impedance counting detection device 510.

[0098] The second syringe 920 provides power for sample dilution. Specifically, the second syringe 920 provides power for the first reaction pool 210, the second reaction pool 220, and the hemoglobin detection unit 400 to add liquid and dilute the sample.

[0099] The second syringe 920 provides power for cleaning the components through which the sample flows. Specifically, the second syringe 920 drives the diluent to clean the first reaction pool 210, the second reaction pool 220, the hemoglobin detection unit 400, the optical sample preparation pipeline 810, the impedance counting sample preparation pipeline 8170 and other components through which the sample flows.

[0100] The second syringe 920 provides power for sample preparation specifically: during reticulocyte detection, the second syringe 920 drives the reticulocyte detection sample from the first reaction pool 210 to be transported to the first sample preparation pipeline 811; during white blood cell classification detection, the second syringe 920 drives the white blood cell classification detection sample from the second reaction pool 220 to be transported to the second sample preparation pipeline 812; during impedance counting detection, the second syringe 920 drives the impedance counting detection sample from the hemoglobin detection unit 400 to the impedance counting sample preparation pipeline 8170.

[0101] The second syringe 920 provides power for filling the diluent reservoir 520 . Specifically, the second syringe 920 drives the diluent into the diluent reservoir 520 .

[0102] The second syringe 920 provides power for pushing the sheath fluid for optical detection. Specifically, during reticulocyte detection, the second syringe 920 drives the sheath fluid into the flow chamber 310 ; during leukocyte classification detection, the second syringe 920 drives the sheath fluid into the flow chamber 310 .

[0103] The detection method of the blood cell analyzer provided in the first embodiment of the present invention includes a sampling step, a diluent delivery step, a sample splitting step, an optical detection step, a hemoglobin detection step, and an impedance counting detection step.

[0104] The sampling step includes: driving the sampling component 100 to absorb the sample to be tested by the first syringe 910 .

[0105] The diluent delivery step includes: driving the diluent from the diluent providing device 1100 to the impedance counting detection sample preparation unit and the hemoglobin detection unit 400 (in this embodiment, the impedance counting detection sample preparation unit and the hemoglobin detection unit 400 are set as one body) through the second syringe 920.

[0106] The sample splitting step includes: driving the sample to be tested in the sampling component 10 to be transported to the optical channel reaction pool 200 and the hemoglobin detection unit 400 respectively through the first syringe 910 .

[0107] The optical detection step includes: providing reagents to the optical channel reaction pool 200 through the first reagent providing device 600 to prepare optical detection samples through the optical channel reaction pool 200; driving the optical detection samples from the optical channel reaction pool 200 to the optical sample preparation pipeline 810 through the second syringe 920; driving the diluent from the diluent providing device 1100 to the flow chamber 310 through the second syringe 920, and at the same time driving the optical detection sample to the flow chamber 310 through the first syringe 910, so that the diluent carries the optical detection sample through the detection area of ​​the flow chamber 310; and detecting the optical detection sample carried by the diluent through the detection area through the optical detection element 320.

[0108] Preferably, in this embodiment, the optical detection step includes a white blood cell classification detection step and a reticulocyte detection step; of course, as an alternative embodiment, the optical detection step may also include only one of the white blood cell classification detection step and the reticulocyte detection step.

[0109] The white blood cell differential detection step includes: providing a white blood cell reagent to the optical channel reaction pool 200 through the first reagent providing device 600 to prepare a white blood cell differential detection sample through the optical channel reaction pool 200; driving the white blood cell differential detection sample from the optical channel reaction pool 200 to the optical sample preparation pipeline 810 through the second syringe 920; driving the diluent from the diluent providing device 1100 to the flow chamber 310 through the second syringe 920, and at the same time driving the white blood cell differential detection sample to the flow chamber 310 through the first syringe 910, so that the diluent carries the white blood cell differential detection sample through the detection area of ​​the flow chamber 310; and detecting the white blood cell differential detection sample carried by the diluent through the detection area through the optical detection element 320.

[0110] The reticulocyte detection steps include: providing a red blood cell reagent to the optical channel reaction pool 200 through the first reagent providing device 600 to prepare a reticulocyte detection sample through the optical channel reaction pool 200; driving the reticulocyte detection sample from the optical channel reaction pool 200 to the optical sample preparation pipeline 810 through the second syringe 920; driving the diluent from the diluent providing device 1100 to the flow chamber 310 through the second syringe 920, and at the same time driving the reticulocyte detection sample to the flow chamber 310 through the first syringe 910, so that the diluent carries the reticulocyte detection sample through the detection area of ​​the flow chamber 310; and detecting the reticulocyte detection sample carried by the diluent through the detection area through the optical detection element 320.

[0111] Preferably, during the reticulocyte detection step, the second syringe 920 drives the reticulocyte detection sample solution from the first reaction reservoir 210 into the first sample preparation line 811, and the first syringe 910 drives the reticulocyte detection sample solution from the first sample preparation line 811 into the flow chamber 310. During the white blood cell differential detection step, the second syringe 920 drives the white blood cell differential detection sample solution from the second reaction reservoir 220 into the second sample preparation line 812, and the first syringe 910 drives the white blood cell differential detection sample solution from the second sample preparation line 812 into the flow chamber 310. The first reaction reservoir 210 and the second reaction reservoir 220 are two independent reaction reservoirs, and the first sample preparation line 811 and the second sample preparation line 812 are two independent sample preparation lines, which helps reduce cross contamination.

[0112] The impedance counting detection step includes: preparing an impedance counting detection sample by an impedance counting detection sample preparation unit (in this embodiment, the impedance counting detection sample preparation unit is a hemoglobin detection unit 400), and performing impedance counting detection on the impedance counting detection sample by an impedance counting detection unit 500.

[0113] The impedance counting detection step includes: preparing an impedance counting detection sample through the hemoglobin detection unit 400; driving the impedance counting detection sample from the hemoglobin detection unit 400 to the impedance counting sample preparation pipeline 8170 through the second syringe 920; driving the impedance counting detection sample from the impedance counting sample preparation pipeline 8170 to the impedance counting detection device 510 through the first syringe 910, and at the same time driving the diluent from the diluent storage tank 520 to the impedance counting detection device 510 through the positive pressure power source 530, so that the impedance counting detection sample flows in the impedance counting detection device 510 under the entrainment of the diluent; and performing impedance counting detection on the impedance counting detection sample entrained by the diluent through the impedance counting detection device 510.

[0114] Preferably, the detection method of the cell analyzer further includes a reservoir filling step, which includes: driving the diluent from the diluent providing device 1100 to the diluent reservoir 520 through the second syringe 920 .

[0115] The hemoglobin detection step includes: providing reagents to the hemoglobin detection unit 400 through the second reagent providing device 700 to prepare a hemoglobin detection sample through the hemoglobin detection unit 400; and detecting the hemoglobin concentration of the hemoglobin detection sample through the hemoglobin detection unit 400.

[0116] Preferably, the detection method of the cell analyzer further includes an optical detection cleaning step, an impedance counting detection cleaning step, a hemoglobin detection cleaning step, and a sampling system cleaning step.

[0117] The optical detection cleaning step includes: driving the diluent from the diluent providing device 1100 to the optical sample preparation pipeline 810 and the optical channel reaction pool 200 through the second syringe 920 to clean the optical sample preparation pipeline 810 and the optical channel reaction pool 200 .

[0118] The optical detection cleaning step is performed after the optical detection step is completed. In this embodiment, the optical detection cleaning step includes a first optical detection cleaning step and a second optical detection cleaning step. The first optical detection cleaning step is performed after the white blood cell differential detection step is completed and is used to clean the second sample preparation pipeline 812 and the second reaction cell 220. The second optical detection cleaning step is performed after the reticulocyte detection step is completed and is used to clean the first sample preparation pipeline 811 and the first reaction cell 210.

[0119] The impedance counting detection cleaning step includes: driving the diluent from the diluent providing device 1100 to the impedance counting detection sample preparation unit (hemoglobin detection unit 400 in this embodiment) through the second syringe 920 to clean the impedance counting detection sample preparation unit;

[0120] The hemoglobin detection and cleaning step includes: driving the diluent from the diluent supply device to the hemoglobin detection unit 400 through the second syringe 920 to clean the hemoglobin detection unit 400;

[0121] The sampling system cleaning step includes: driving the diluent from the diluent providing device 1100 to the first syringe 910 and the sampling component 100 through the second syringe 920 to clean the first syringe 910, the sampling component 100 and the pipeline connected between the first syringe 910 and the sampling component 100.

[0122] The basic working process of the blood cell analyzer provided in this embodiment is as follows:

[0123] 1) The first syringe 910 draws the sample to be tested from the test tube, the first metering pump 610 and the second metering pump 620 add the first reagent and the second reagent to the first reaction well 210, respectively, the third metering pump 630 and the fourth metering pump 640 add the third reagent and the fourth reagent to the second reaction well 220, respectively, and simultaneously the first syringe 910 adds the sample to be tested to the first reaction well 210 and the second reaction well 220 for sample incubation reaction;

[0124] 2) Open the first control valve 8121 and the third control valve 8201, and add the diluent to the hemoglobin detection unit 400 (the reaction pool shared by the hemoglobin detection and impedance counting detection) through the second syringe 920. Simultaneously, the first syringe 910 adds the sample to be tested to the hemoglobin detection unit 400 for sample incubation reaction;

[0125] 3) Open the first control valve 8121, the seventh control valve 8161, and the eighth control valve 8181, and draw the impedance counting test sample from the hemoglobin detection unit 400 into the impedance counting sample preparation line 8170 via the second syringe 920; then, the fifth metering pump 710 adds the fifth reagent to the hemoglobin detection unit 400 for sample incubation reaction;

[0126] 4) The hemoglobin detection unit 400 begins measuring hemoglobin concentration. Simultaneously, the air valve 570 is opened, and the diluent reservoir 520, driven by the positive pressure power source 530, supplies sheath fluid to the impedance counting detection device 510. The ninth control valve 8211 and the fifth controllable valve 862 are opened and regulated, driving the impedance counting detection sample in the impedance counting sample preparation pipeline 8170 into the gemstone hole via the first syringe 910 to initiate impedance counting measurement.

[0127] 5) Open the first control valve 8121 and the third control valve 8201, and add diluent to the hemoglobin detection unit 400 through the second syringe 920 to clean the hemoglobin detection unit 400;

[0128] 6) Open the first control valve 8121, the second controllable valve 841, and the third controllable valve 851, and draw the white blood cell differential detection sample from the second reaction pool 220 into the second sample preparation pipeline 812 through the second syringe 920;

[0129] 7) Open the first control valve 8121, the second control valve 8122, and the first liquid discharge control valve 8221 to push the sheath fluid into the flow chamber 310 via the second syringe 920. Simultaneously, open and regulate the sixth control valve 861, the ninth control valve 8211, and the fifth controllable valve 862 to drive the white blood cell differential test sample into the flow chamber 310 via the first syringe 910 to initiate white blood cell differential measurement.

[0130] 8) Using the second syringe 920 to drive the diluent to clean the delivery pipeline (the pipeline from the second controllable valve 841 through the third controllable valve 851 to the second reaction tank 220 ) and the second reaction tank 220 of the leukocyte differential detection sample;

[0131] 9) Open the first control valve 8121, the second controllable valve 841, and the first controllable valve 831, and draw the reticulocyte test sample from the first reaction pool 210 into the first sample preparation pipeline 811 through the second syringe 920;

[0132] 10) Open the first control valve 8121, the second control valve 8122, and the first liquid discharge control valve 8221 to push the sheath fluid into the flow chamber 310 via the second syringe 920. Simultaneously, open and regulate the sixth control valve 861, the ninth control valve 8211, and the fifth controllable valve 862 to drive the reticulocyte test sample into the flow chamber 310 via the first syringe 910 to initiate reticulocyte measurement.

[0133] 11) Using the second syringe 920 to drive the diluent to clean the reticulocyte test sample delivery pipeline (the pipeline from the second controllable valve 841 through the first controllable valve 831 to the first reaction tank 210) and the first reaction tank 210;

[0134] 12) Open the first control valve 8121 and the fourth control valve 8141, and drive the diluent to be delivered to the diluent reservoir 520 through the second syringe 920 to fill the diluent reservoir 520;

[0135] 13) Open the first control valve 8121, the fifth control valve 8151 and the fifth controllable valve 862, and use the second syringe 920 to drain liquid to clean the sampling delivery tube 820 and the sampling component 100;

[0136] 14) The measurement is completed.

[0137] Example 2:

[0138] The main difference between this embodiment and the first embodiment is that the arrangement scheme of the optical sample preparation pipeline 810 is different. Figure 1 and Figure 2 As shown, in the first embodiment, the optical sample preparation pipeline 810 includes a first sample preparation pipeline 811 and a second sample preparation pipeline 812. In the white blood cell classification detection step and the reticulocyte detection step, the white blood cell classification detection sample and the reticulocyte detection sample respectively use independent optical sample preparation pipelines 810; and as shown in FIG. Figure 4 As shown, in this embodiment, the optical sample preparation pipeline 810 only includes a first sample preparation pipeline 811. In the white blood cell classification detection step and the reticulocyte detection step, the white blood cell classification detection sample and the reticulocyte detection sample share the same sample preparation pipeline.

[0139] In this embodiment, the first sample preparation pipeline 811 is connected to the first reaction pool 210 and the second reaction pool 220 respectively. The second syringe 920 is used to drive the reticulocyte detection sample from the first reaction pool 210 to the first sample preparation pipeline 811 when performing reticulocyte detection, and to drive the white blood cell classification detection sample from the second reaction pool 220 to the first sample preparation pipeline 811 when performing white blood cell classification detection. The first syringe 910 is used to drive the reticulocyte detection sample from the first sample preparation pipeline 811 to the flow chamber 310 when performing reticulocyte detection, and to drive the white blood cell classification detection sample from the first sample preparation pipeline 811 to the flow chamber 310 when performing white blood cell classification detection.

[0140] In this embodiment, the delivery pipeline assembly 800 further includes a first connecting pipeline 830, a second connecting pipeline 840, a third connecting pipeline 850, a fourth connecting pipeline 860, a first connector 880, a third connector 8100, and a fifth connector 8270. One end of the first sample preparation pipeline 811 is connected to the flow chamber 310, and the other end of the first sample preparation pipeline 811 is connected to the first reaction cell 210 via the first connecting pipeline 830 and to the second reaction cell 220 via the third connecting pipeline 850. The second syringe 920 is connected to the first sample preparation pipeline 811 via the second connecting pipeline 840, and the first syringe 910 is connected to the first sample preparation pipeline 811 via the fourth connecting pipeline 860.

[0141] The first connector 880 has a first interface a, a second interface b, and a third interface c that are interconnected. The first interface a is connected to the flow chamber 310, the second interface b is connected to one end of the first sample preparation line 811, and the other end of the first sample preparation line 811 is connected to the first connecting line 830 or the third connecting line 850. The first connecting line 830 is connected to the third connecting line 850 via a fifth connector 8270. The first syringe 910 is connected to the first sample preparation line 811 via a third connector 8100. The third interface c is connected to the second syringe 920 via a second connecting line 840. The third connector 8100 is preferably a three-way connector, that is, the first syringe 910 is connected to the optical sample preparation line 810 via the three-way connector. In this embodiment, the three-way connector (third connector 8100) is provided between the flow chamber 310 and the optical channel reaction cell 200, that is, the interface between the first syringe 910 and the optical sample preparation line 810 is located upstream of the optical detection channel.

[0142] Except for the above differences, other parts of the blood cell analyzer and the detection method of the cell analyzer provided in this embodiment can be designed with reference to the corresponding embodiment 1 and will not be described in detail here.

[0143] Example 3:

[0144] The main difference between this embodiment and the second embodiment is that the first sample preparation pipeline 811 and the first syringe 910 are arranged in different positions. Figure 4 As shown, in the second embodiment, the first sample preparation pipeline 811 and the first syringe 910 are located upstream of the flow chamber 310. When the first syringe 910 is working, the reticulocyte test sample and the white blood cell classification test sample upstream are pushed into the flow chamber 310 for testing; and as shown in FIG. Figure 5 As shown, in this embodiment, the first sample preparation pipeline 811 and the first syringe 910 are located downstream of the flow chamber 310. When the first syringe 910 is in operation, it pushes the reticulocyte test sample and the white blood cell classification test sample downstream into the flow chamber 310 for testing.

[0145] The delivery pipeline assembly 800 further includes a first connector 880 and a sixth connector 8130. The first connector 880 has a first port a, a second port b, and a third port c that are interconnected. The first port a is connected to the flow chamber 310, the second port b is connected to the first connecting line 830 or the third connecting line 850, the first connecting line 830 is connected to the third connecting line 850 via a fifth connector 8270, and the third port c is connected to one end of the first sample preparation line 811. The other end of the first sample preparation line 811 is connected to the second connecting line 840 and the fourth connecting line 860, respectively, via a sixth connector 8130. The sixth connector 8130 is preferably a three-way connector, i.e., the first syringe 910 is connected to the optical sample preparation line 810 via the three-way connector. The three-way connector is provided between the flow chamber 310 and the second syringe 920.

[0146] This embodiment can prevent the white blood cell classification test sample or the reticulocyte test sample from entering the three-way connector above the first syringe 910 (i.e., the sixth connector 8130 connecting the first sample preparation pipeline 811 and the first syringe 910), and there is no need to clean the branch of the sixth connector 8130, thereby reducing the difficulty of cleaning the first sample preparation pipeline 811.

[0147] In addition to the above differences, other parts of the blood cell analyzer and the detection method of the cell analyzer provided in this embodiment can refer to the corresponding designs of the first and second embodiments and will not be described in detail here.

[0148] Example 4:

[0149] The main difference between this embodiment and the first to third embodiments is that the arrangement scheme of the optical channel reaction pool 200 is different. Figure 1 、 Figure 2 、 Figure 4 and Figure 5As shown, in Examples 1 to 3, the reticulocyte detection sample and the leukocyte classification detection sample are incubated in two independent reaction pools (the first reaction pool 210 and the second reaction pool 220) respectively; Figure 6 As shown, in this embodiment, the reticulocyte detection sample and the leukocyte classification detection sample are incubated and generated in the same reaction tank (the first reaction tank 210 ).

[0150] In this embodiment, there is only one optical channel reaction pool 200, namely, the optical channel reaction pool 200 includes only a first reaction pool 210. The first metering pump 610, the second metering pump 620, the third metering pump 630, and the fourth metering pump 640 are all connected to the first reaction pool 210. The first reaction pool 210 is used to provide a reaction field for the test sample and the white blood cell reagent to prepare a white blood cell differential detection sample, and is used to provide a reaction field for the test sample and the red blood cell reagent to prepare a reticulocyte detection sample.

[0151] Taking into account the problem of cross-contamination of fluorescent reagent residues between optical channels, in this embodiment, a fluorescent platform is not used in the white blood cell classification test (for example, a chemical staining platform is used, a hemolytic agent is used, but no fluorescent staining is used), and a fluorescent platform is used for reticulocyte detection. In this way, the white blood cell classification test and the reticulocyte detection share a reaction pool without cross-contamination of fluorescent reagent residues.

[0152] Specifically, in this embodiment, a fluorescent reagent is used in the preparation of the reticulocyte detection sample, and a fluorescent reagent is not used in the preparation of the white blood cell classification detection sample, that is, the red blood cell reagent delivered by the first reagent providing device 600 to the first reaction pool 210 includes a fluorescent reagent, and the white blood cell reagent delivered by the first reagent providing device 600 to the first reaction pool 210 does not include a fluorescent reagent.

[0153] The optical sample preparation pipeline 810 includes a first sample preparation pipeline 811 connected to the first reaction pool 210. The second syringe 920 is used to drive and transport the reticulocyte detection sample from the first reaction pool 210 to the first sample preparation pipeline 811 when performing reticulocyte detection and to drive and transport the white blood cell classification detection sample from the first reaction pool 210 to the first sample preparation pipeline 811 when performing white blood cell classification detection. The first syringe 910 is used to drive and transport the reticulocyte detection sample from the first sample preparation pipeline 811 to the flow chamber 310 when performing reticulocyte detection and to drive and transport the white blood cell classification detection sample from the first sample preparation pipeline 811 to the flow chamber 310 when performing white blood cell classification detection.

[0154] The delivery pipeline assembly 800 also includes a first connecting pipeline 830, a second connecting pipeline 840, a fourth connecting pipeline 860, a first connector 880 and a third connector 8100. One end of the first sample preparation pipeline 811 is connected to the flow chamber 310, and the other end is connected to the first reaction pool 210 through the first connecting pipeline 830. The second syringe 920 is connected to the first sample preparation pipeline 811 through the second connecting pipeline 840, and the first syringe 910 is connected to the first sample preparation pipeline 811 through the fourth connecting pipeline 860.

[0155] The first connector 880 has a first interface a, a second interface b and a third interface c that are interconnected. The first interface a is connected to the flow chamber 310, the second interface b is connected to one end of the first sample preparation pipeline 811, and the other end of the first sample preparation pipeline 811 is connected to the first connecting pipeline 830 and the fourth connecting pipeline 860 respectively through the third connector 8100. The third interface c is connected to the second syringe 920 through the second connecting pipeline 840.

[0156] In addition to the above differences, other parts of the blood cell analyzer and the detection method of the cell analyzer provided in this embodiment can refer to the corresponding designs of the first, second and third embodiments and will not be described in detail here.

[0157] Embodiment 5:

[0158] The main difference between this embodiment and the fourth embodiment is that the first sample preparation pipeline 811 and the first syringe 910 are arranged in different positions. Figure 6 As shown, in the fourth embodiment, the first sample preparation pipeline 811 and the first syringe 910 are located upstream of the flow chamber 310. When the first syringe 910 is working, the reticulocyte test sample and the white blood cell classification test sample upstream are pushed into the flow chamber 310 for testing; and as shown in FIG. Figure 7 As shown, in this embodiment, the first sample preparation pipeline 811 and the first syringe 910 are located downstream of the flow chamber 310. When the first syringe 910 is in operation, it pushes the reticulocyte test sample and the white blood cell classification test sample downstream into the flow chamber 310 for testing.

[0159] The transport pipeline assembly 800 also includes a first connector 880 and a sixth connector 8130. The first connector 880 has a first interface a, a second interface b and a third interface c that are interconnected. The first interface a is connected to the flow chamber 310, the second interface b is connected to the first connecting pipeline 830, and the third interface c is connected to one end of the first sample preparation pipeline 811. The other end of the first sample preparation pipeline 811 is connected to the second connecting pipeline 840 and the fourth connecting pipeline 860 respectively through the sixth connector 8130.

[0160] In addition to the above differences, other parts of the blood cell analyzer and the detection method of the cell analyzer provided in this embodiment can refer to the corresponding designs of the fourth embodiment and will not be described in detail here.

[0161] Example 6:

[0162] The main difference between this embodiment and embodiments 1 to 5 is that the sheath fluid pushing method in the impedance counting detection is different. Figure 1 and Figures 3 to 7 As shown, in the first to fifth embodiments, a positive pressure power source 530 is used to drive the sheath fluid in the diluent reservoir 520 to be transported to the impedance counting detection device 510; and as shown in FIG. Figure 8 As shown, in this embodiment, the second syringe 920 is used to drive the sheath fluid in the diluent providing device 1100 to be transported to the impedance counting detection device 510.

[0163] Specifically, in this embodiment, the diluent reservoir 520 and the positive pressure power source 530 are not provided. The impedance counting detection unit 500 includes an impedance counting detection device 510, which is connected to the second syringe 920 and the diluent providing device 1100 via the third diluent delivery line 8140. One end of the impedance counting sample preparation line 8170 is connected to the impedance counting detection device 510 and is connected to the second syringe 920 via the first delivery line 8160 (in this embodiment, the first delivery line 8160 is indirectly connected to the second syringe 920 via the third diluent delivery line 8140 and the first diluent delivery line 8120). The other end of the impedance counting sample preparation line 8170 is connected to the hemoglobin detection unit 400 via the second delivery line 8180 (in this embodiment, the second delivery line 8180 is indirectly connected to the hemoglobin detection unit 400 via the second diluent delivery line 8200) and is connected to the first syringe 910 via the third delivery line 8210.

[0164] In this embodiment, the second syringe 920 is reused to drive the diluent from the diluent providing device 1100 to be transported to the impedance counting detection device 510, so that the impedance counting detection sample flows in the impedance counting detection device 510 under the influence of the diluent. The second syringe 920 is also used to drive the impedance counting detection sample from the hemoglobin detection unit 400 to be transported to the impedance counting sample preparation pipeline 8170. The first syringe 910 is reused to drive the impedance counting detection sample from the impedance counting sample preparation pipeline 8170 to be transported to the impedance counting detection device 510.

[0165] Specifically, the second inlet h of the impedance counting detection device 510 is connected to the first diluent delivery line 8120 via the third diluent delivery line 8140. By opening and regulating the first control valve 8121 and the fourth control valve 8141, the second syringe 920 can drive the sheath fluid to be delivered into the impedance counting detection device 510.

[0166] In this embodiment, the impedance counting detection sample preparation unit is also integrated with the hemoglobin detection unit 400 .

[0167] In this embodiment, the impedance counting detection step includes: preparing an impedance counting detection sample through the hemoglobin detection unit 400; driving the impedance counting detection sample from the hemoglobin detection unit 400 to the impedance counting sample preparation pipeline 8170 through the second syringe 920; driving the impedance counting detection sample from the impedance counting sample preparation pipeline 8170 to the impedance counting detection device 510 through the first syringe 910, and at the same time driving the diluent from the diluent providing device 1100 to the impedance counting detection device 510 through the second syringe 920, so that the impedance counting detection sample flows in the impedance counting detection device 510 under the entrainment of the diluent; and performing impedance counting detection on the impedance counting detection sample entrained by the diluent through the impedance counting detection device 510.

[0168] In addition to the above differences, other parts of the blood cell analyzer and the detection method of the cell analyzer provided in this embodiment can be designed with reference to any one of the first to fifth embodiments, and will not be described in detail here.

[0169] Embodiment seven:

[0170] The main difference between this embodiment and embodiments 1 to 6 is that the impedance counting detection method is different. Figure 1 and Figures 3 to 8 As shown, in the first to sixth embodiments, the impedance counting detection unit 500 adopts the sheath flow impedance solution; Figure 9 As shown, in this embodiment, the impedance counting detection unit 500 adopts a common impedance solution.

[0171] Specifically, in this embodiment, the impedance counting detection unit 500 includes an impedance counting detection device 510 and a negative pressure power source 560, and the impedance counting detection sample preparation unit is integrated with the impedance counting detection device 510, that is, in addition to being used for impedance counting detection, the impedance counting detection device 510 is also used as an impedance counting detection sample preparation unit to provide a reaction field for the sample to be tested and the diluent to prepare the impedance counting detection sample; the first syringe 910 is also used to drive the sample to be tested in the sampling component 100 to be transported into the impedance counting detection device 510 to achieve sample separation; the negative pressure power source 560 is connected to the impedance counting detection device 510 to provide flow power for the detection of the impedance counting detection sample in the impedance counting detection device 510 (that is, drive the impedance counting detection sample from the front pool of the impedance counting detection device 510 to the rear pool).

[0172] The impedance counting detection device 510 is connected to the second syringe 920 and the diluent providing device 1100 via the third diluent delivery pipeline 8140 . The second syringe 920 is also used to drive the diluent from the diluent providing device 100 to be delivered into the impedance counting detection device 510 .

[0173] In this embodiment, a fifth liquid discharge control valve 8261 is provided on the fifth liquid discharge pipeline 8260. By controlling the fifth liquid discharge control valve 8261, the fifth liquid discharge pipeline 8260 can be controlled to be on or off.

[0174] In this embodiment, during impedance counting detection, there is no need to use the first syringe 910 to push the sample. The first syringe 910 of this embodiment is reused for sampling, blood separation, and optical channel sample pushing; the second syringe 920 is reused for adding liquid to the impedance counting detection device 510 to dilute the sample to be tested and add liquid for cleaning.

[0175] In this embodiment, the impedance counting detection step includes: preparing an impedance counting detection sample through the front pool of the impedance counting detection device 510; driving the impedance counting detection sample from the front pool to the rear pool of the impedance counting detection device 510 through the negative pressure power source 560, and performing impedance counting detection on the impedance counting detection sample through the impedance counting detection device 510.

[0176] In addition to the above differences, other parts of the blood cell analyzer and the detection method of the cell analyzer provided in this embodiment can be designed with reference to any one of the first to sixth embodiments, and will not be described in detail here.

[0177] The above description is only a preferred embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made by using the contents of the present invention description and drawings under the inventive concept of the present invention, or direct / indirect application in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A cell analyzer, characterized in that include: A sampling component, used for collecting samples to be tested; An optical channel reaction pool, which is used to provide a reaction site for the sample to be tested and the reagent to prepare an optical detection sample; an optical detection unit, the optical detection unit comprising a flow chamber and an optical detection element, the flow chamber having a diluent inlet, a sample inlet, a first outlet, and a detection zone for allowing the optical detection sample to pass through under the influence of the diluent, the diluent inlet, the sample inlet, and the first outlet being respectively connected to the detection zone, and the optical detection element being used to detect the optical detection sample carried by the diluent through the detection zone; A hemoglobin detection unit, the hemoglobin detection unit is used to provide a reaction site for the test sample and the reagent to prepare a hemoglobin detection sample, and is used to detect the hemoglobin concentration of the hemoglobin detection sample; An impedance counting detection sample preparation unit, the impedance counting detection sample preparation unit is used to provide a reaction field for the sample to be tested and the diluent to prepare an impedance counting detection sample; An impedance counting detection unit, configured to perform impedance counting detection on an impedance counting detection sample; a first reagent providing device, the first reagent providing device being used to provide reagents for the optical channel reaction cell; a second reagent providing device, the second reagent providing device being used to provide reagents for the hemoglobin detection unit; a diluent providing device, the diluent providing device being used to provide a diluent; a delivery pipeline assembly, the delivery pipeline assembly comprising an optical sample preparation pipeline, a first diluent delivery pipeline, a second diluent delivery pipeline, and a third diluent delivery pipeline, the optical sample preparation pipeline being connected to the sample inlet and the optical channel reaction pool, respectively; the first diluent delivery pipeline being connected to the diluent inlet and the diluent supply device, respectively; the second diluent delivery pipeline being connected to the diluent supply device and the hemoglobin detection unit, respectively; and the third diluent delivery pipeline being connected to the diluent supply device and the impedance counting detection unit, respectively; A fluid power device, comprising a first syringe and a second syringe, wherein the measuring range of the first syringe is smaller than the measuring range of the second syringe; The first syringe is connected to the sampling component to drive the sampling component to absorb the sample to be tested, and is used to drive the sample to be tested in the sampling component to be respectively transported to the hemoglobin detection unit, the optical channel reaction pool, and the impedance counting detection sample preparation unit for reaction. The first syringe is also connected to the optical sample preparation pipeline to drive the optical detection sample from the optical sample preparation pipeline to be transported into the flow chamber; The second syringe is connected to the first diluent delivery pipeline for driving the diluent from the diluent supply device to the flow chamber, the second syringe is also connected to the optical sample preparation pipeline for driving the optical detection sample from the optical channel reaction pool to the optical sample preparation pipeline, the second syringe is also connected to the second diluent delivery pipeline for driving the diluent from the diluent supply device to the hemoglobin detection unit, the second syringe is also connected to the impedance counting detection sample preparation unit for driving the diluent from the diluent supply device to the impedance counting detection sample preparation unit, and the second syringe is also connected to the third diluent delivery pipeline for driving the diluent from the diluent supply device to the impedance counting detection unit.

2. The cell analyzer according to claim 1, wherein The optical channel reaction pool includes a first reaction pool, which is used to provide a reaction site for the sample to be tested and the red blood cell reagent to prepare a reticulocyte detection sample; The optical detection element is used to perform reticulocyte detection on the reticulocyte detection sample carried by the diluent through the detection area, the first syringe is used to drive the reticulocyte detection sample from the optical sample preparation pipeline into the flow chamber, and the second syringe is used to drive the reticulocyte detection sample from the first reaction pool into the optical sample preparation pipeline.

3. The cell analyzer according to claim 1, wherein The optical channel reaction pool includes a second reaction pool, which is used to provide a reaction site for the sample to be tested and the white blood cell reagent to prepare a white blood cell differential detection sample; the optical detection element is also used to perform white blood cell differential detection on the white blood cell differential detection sample carried by the diluent through the detection area; the first syringe is also used to drive the white blood cell differential detection sample from the optical sample preparation pipeline into the flow chamber, and the second syringe is also used to drive the white blood cell differential detection sample from the second reaction pool into the optical sample preparation pipeline.

4. The cell analyzer according to claim 1, wherein The optical channel reaction pool includes a first reaction pool, which is used to provide a reaction site for the test sample and the red blood cell reagent to prepare a reticulocyte test sample, and to provide a reaction site for the test sample and the white blood cell reagent to prepare a white blood cell differential test sample; The optical detection element is used to perform reticulocyte detection on the reticulocyte detection sample carried by the diluent through the detection zone and to perform leukocyte classification detection on the leukocyte classification detection sample carried by the diluent through the detection zone; the first syringe is used to drive the reticulocyte detection sample from the optical sample preparation pipeline into the flow chamber and to drive the leukocyte classification detection sample from the optical sample preparation pipeline into the flow chamber; the second syringe is used to drive the reticulocyte detection sample from the first reaction pool into the optical sample preparation pipeline and to drive the leukocyte classification detection sample from the second reaction pool into the optical sample preparation pipeline; or, The optical channel reaction pool includes a first reaction pool and a second reaction pool. The first reaction pool and the second reaction pool are two independent reaction pools. The first reaction pool is used to provide a reaction site for the test sample and the red blood cell reagent to prepare the reticulocyte test sample. The second reaction pool is used to provide a reaction site for the test sample and the white blood cell reagent to prepare the white blood cell differential test sample. The optical detection element is used to perform reticulocyte detection on the reticulocyte detection sample carried by the diluent through the detection area and to perform leukocyte classification detection on the leukocyte classification detection sample carried by the diluent through the detection area. The first syringe is used to drive the reticulocyte detection sample from the optical sample preparation pipeline into the flow chamber and to drive the leukocyte classification detection sample from the optical sample preparation pipeline into the flow chamber. The second syringe is used to drive the reticulocyte detection sample from the first reaction pool into the optical sample preparation pipeline and to drive the leukocyte classification detection sample from the second reaction pool into the optical sample preparation pipeline.

5. The cell analyzer according to claim 4, wherein: The optical channel reaction pool includes the first reaction pool and the second reaction pool, and the delivery pipeline assembly also includes a first connecting pipeline, a second connecting pipeline, a third connecting pipeline and a fourth connecting pipeline. The first connecting pipeline is provided with a first controllable valve, the second connecting pipeline is provided with a second controllable valve, the third connecting pipeline is provided with a third controllable valve, and the fourth connecting pipeline is provided with a fourth controllable valve. The first reaction pool is connected to the optical sample preparation pipeline through the first connecting pipeline, the second reaction pool is connected to the optical sample preparation pipeline through the third connecting pipeline, the second syringe is connected to the optical sample preparation pipeline through the second connecting pipeline, and the first syringe is connected to the optical sample preparation pipeline through the fourth connecting pipeline.

6. The cell analyzer according to any one of claims 2, 3 and 4, wherein: The optical channel reaction pool includes the first reaction pool or the second reaction pool, and the transport pipeline assembly also includes a first connecting pipeline, a second connecting pipeline and a fourth connecting pipeline. The first connecting pipeline is provided with a first controllable valve, the second connecting pipeline is provided with a second controllable valve, and the fourth connecting pipeline is provided with a fourth controllable valve. The optical channel reaction pool is connected to the optical sample preparation pipeline through the first connecting pipeline, the second syringe is connected to the optical sample preparation pipeline through the second connecting pipeline, and the first syringe is connected to the optical sample preparation pipeline through the fourth connecting pipeline.

7. The cell analyzer according to claim 5, wherein The fourth connecting pipeline is further provided with a fifth controllable valve, and the fifth controllable valve is located between the first syringe and the fourth controllable valve. The sampling component is connected to the first syringe via the fifth controllable valve.

8. The cell analyzer according to claim 5 or 7, characterized in that: The second connecting pipeline is also connected to the first diluent delivery pipeline, and the second syringe is further used to drive the diluent from the diluent providing device to the optical sample preparation pipeline and the optical channel reaction pool.

9. The cell analyzer according to any one of claims 1 to 5, characterized in that: The optical channel reaction pool includes at least two reaction pools, wherein one reaction pool is solely used to provide a reaction field for the sample to be tested and the red blood cell reagent to prepare a reticulocyte detection sample. The number of the optical sample preparation pipelines is at least two, and all of the optical sample preparation pipelines are connected to the sample inlet, the optical channel reaction pool, the first syringe, and the second syringe. One of the optical sample preparation pipelines is connected to the reaction pool for preparing the reticulocyte detection sample and is solely used to allow the reticulocyte detection sample and diluent to flow through.

10. The cell analyzer according to any one of claims 1 to 5, characterized in that: There is one optical sample preparation pipeline, and the second delivery pipeline assembly also includes a first connector, the first connector having a first interface, a second interface, and a third interface that are interconnected, the first interface being connected to the sample inlet; the second interface being connected to one end of the optical sample preparation pipeline, the third interface being connected to the second syringe, and the other end of the optical sample preparation pipeline being connected to all the optical channel reaction cells respectively.

11. The cell analyzer according to claim 10, characterized in that: The first syringe is connected to the optical sample preparation pipeline through a three-way connector, and the three-way connector is arranged between the flow chamber and the optical channel reaction pool.

12. The cell analyzer according to claim 10, wherein: The first syringe is connected to the optical sample preparation pipeline through a three-way connector, and the three-way connector is arranged between the flow chamber and the second syringe.

13. The cell analyzer according to any one of claims 1 to 5, characterized in that: The first diluent delivery pipeline is provided with a first control valve and a second control valve, the first control valve is provided between the second syringe and the second control valve, and the diluent providing device is connected to the first control valve; The second diluent delivery pipeline is provided with a third control valve, the third diluent delivery pipeline is provided with a fourth control valve, and the second diluent delivery pipeline and the third diluent delivery pipeline are both connected to the diluent supply device through the first diluent delivery pipeline.

14. The cell analyzer according to any one of claims 1 to 5, wherein: The delivery pipeline assembly also includes a fourth diluent delivery pipeline, on which a fifth control valve is provided. The second syringe is connected to the diluent providing device and the first syringe respectively through the fourth diluent delivery pipeline to drive the diluent from the diluent providing device to the first syringe.

15. The cell analyzer according to any one of claims 1 to 5, characterized in that: The impedance counting detection sample preparation unit is integrally provided with the hemoglobin detection unit, and the hemoglobin detection unit is further used to provide a reaction site for the sample to be tested and the diluent to prepare the impedance counting detection sample; The impedance counting detection unit includes an impedance counting detection device, a diluent reservoir, a positive pressure power source, and a fifth diluent delivery pipeline. The fifth diluent delivery pipeline is provided with a sixth control valve. The delivery pipeline assembly also includes an impedance counting sample preparation pipeline, a first delivery pipeline, a second delivery pipeline, and a third delivery pipeline. The first delivery pipeline is provided with a seventh control valve, the second delivery pipeline is provided with an eighth control valve, and the third delivery pipeline is provided with a ninth control valve. The positive pressure power source is connected to the diluent reservoir, the diluent reservoir is connected to the impedance counting detection device via the fifth diluent delivery pipeline, and is connected to the second syringe and the diluent providing device via the third diluent delivery pipeline; One end of the impedance counting sample preparation pipeline is connected to the impedance counting detection device and is connected to the second syringe through the first delivery pipeline. The other end of the impedance counting sample preparation pipeline is connected to the hemoglobin detection unit through the second delivery pipeline and is connected to the first syringe through the third delivery pipeline. The second syringe is also used to drive the diluent from the diluent providing device to the diluent reservoir and to drive the impedance counting detection sample from the hemoglobin detection unit to the impedance counting sample preparation pipeline. The positive pressure power source is used to drive the diluent from the diluent reservoir to the impedance counting detection device so that the impedance counting detection sample flows in the impedance counting detection device under the influence of the diluent; the first syringe is also used to drive the impedance counting detection sample from the impedance counting sample preparation pipeline to the impedance counting detection device.

16. The cell analyzer according to any one of claims 1 to 5, wherein: The impedance counting detection sample preparation unit is integrally provided with the hemoglobin detection unit, and the hemoglobin detection unit is further used to provide a reaction site for the sample to be tested and the diluent to prepare the impedance counting detection sample; The impedance counting detection unit includes an impedance counting detection device, and the delivery pipeline assembly also includes an impedance counting sample preparation pipeline, a first delivery pipeline, a second delivery pipeline, and a third delivery pipeline. The first delivery pipeline is provided with a seventh control valve, the second delivery pipeline is provided with an eighth control valve, and the third delivery pipeline is provided with a ninth control valve; The impedance counting detection device is connected to the second syringe and the diluent providing device via the third diluent delivery pipeline; One end of the impedance counting sample preparation pipeline is connected to the impedance counting detection device and is connected to the second syringe through the first delivery pipeline. The other end of the impedance counting sample preparation pipeline is connected to the hemoglobin detection unit through the second delivery pipeline and is connected to the first syringe through the third delivery pipeline. The second syringe is also used to drive the impedance counting detection sample from the hemoglobin detection unit to the impedance counting sample preparation pipeline, and the second syringe is also used to drive the diluent from the diluent providing device to the impedance counting detection device, so that the impedance counting detection sample flows in the impedance counting detection device under the influence of the diluent, and the first syringe is also used to drive the impedance counting detection sample from the impedance counting sample preparation pipeline to the impedance counting detection device.

17. The cell analyzer according to claim 15, wherein The first syringe is connected to the impedance counting sample preparation pipeline and the optical detection sample preparation pipeline respectively through the ninth control valve.

18. The cell analyzer according to any one of claims 1 to 5, wherein The impedance counting detection unit includes an impedance counting detection device and a negative pressure power source. The impedance counting detection sample preparation unit is integrally provided with the impedance counting detection device. The first syringe is further used to drive the sample to be tested in the sampling component to be transported into the impedance counting detection device. The negative pressure power source is connected to the impedance counting detection device to provide flow power for the detection of the impedance counting detection sample in the impedance counting detection device. The impedance counting detection device is connected to the second syringe and the diluent providing device through the third diluent delivery pipeline, and the second syringe is also used to drive the diluent from the diluent providing device to be delivered to the impedance counting detection unit.

19. The cell analyzer according to any one of claims 1 to 5, wherein: The fluid power device further includes a first motor for driving the first syringe and a second motor for driving the second syringe. The first motor and the second motor are two motors independently provided.

20. The cell analyzer according to any one of claims 1 to 5, wherein The first reagent supply device further includes a first metering pump, a second metering pump, a third metering pump and a fourth metering pump, wherein the first metering pump is used to provide the first reagent to the optical channel reaction cell; the second metering pump is used to provide the second reagent to the optical channel reaction cell; the third metering pump is used to provide the third reagent to the optical channel reaction cell; and the fourth metering pump is used to provide the fourth reagent to the optical channel reaction cell; and / or, The second reagent supply device includes a fifth metering pump for supplying reagents to the hemoglobin detection unit, and the fifth metering pump is used to supply reagents to the optical channel reaction pool and the hemoglobin detection unit.

21. A detection method for a cell analyzer, comprising a sampling step, a diluent delivery step, a sample splitting step, an optical detection step, an impedance counting detection step, and a hemoglobin detection step, wherein: The sampling step includes: driving the sampling component to absorb the sample to be tested by the first syringe; The diluent delivery step includes: driving the diluent from the diluent supply device to deliver the diluent to the impedance counting detection sample preparation unit and the hemoglobin detection unit respectively through a second syringe, wherein the range of the second syringe is greater than the range of the first syringe; The sample splitting step includes: driving the sample to be tested in the sampling component to be transported to the optical channel reaction pool, the hemoglobin detection unit and the impedance counting detection sample preparation unit respectively through the first syringe; The optical detection step includes: providing a reagent to the optical channel reaction pool by a first reagent supply device to prepare an optical detection sample through the optical channel reaction pool; driving the optical detection sample from the optical channel reaction pool to the optical sample preparation pipeline by a second syringe; driving a diluent from the diluent supply device to the flow chamber by the second syringe, and simultaneously driving the optical detection sample to the flow chamber by the first syringe, so that the diluent carries the optical detection sample through the detection zone of the flow chamber; and detecting the optical detection sample carried by the diluent through the detection zone by the optical detection element. The impedance counting detection step includes: preparing an impedance counting detection sample by the impedance counting detection sample preparation unit, and performing impedance counting detection on the impedance counting detection sample by the impedance counting detection unit; The hemoglobin detection step includes: providing reagents to the hemoglobin detection unit through a second reagent providing device to prepare a hemoglobin detection sample through the hemoglobin detection unit; and detecting the hemoglobin concentration of the hemoglobin detection sample through the hemoglobin detection unit.

22. The detection method of the cell analyzer according to claim 21, wherein: The optical detection step includes a white blood cell classification detection step and / or a reticulocyte detection step, The white blood cell differential detection step includes: providing a white blood cell reagent to the optical channel reaction pool by the first reagent providing device, so as to prepare a white blood cell differential detection sample through the optical channel reaction pool; driving the white blood cell differential detection sample from the optical channel reaction pool to the optical sample preparation pipeline by the second syringe; driving a diluent from the diluent providing device to the flow chamber by the second syringe, and simultaneously driving the white blood cell differential detection sample to the flow chamber by the first syringe, so that the diluent carries the white blood cell differential detection sample through the detection area of ​​the flow chamber; and detecting the white blood cell differential detection sample carried by the diluent through the detection area by the optical detection element; The reticulocyte detection step includes: providing a red blood cell reagent to the optical channel reaction pool through the first reagent providing device to prepare a reticulocyte detection sample through the optical channel reaction pool; driving the reticulocyte detection sample from the optical channel reaction pool to the optical sample preparation pipeline through the second syringe; driving the diluent from the diluent providing device to the flow chamber through the second syringe, and simultaneously driving the reticulocyte detection sample to the flow chamber through the first syringe, so that the diluent carries the reticulocyte detection sample through the detection area of ​​the flow chamber; and detecting the reticulocyte detection sample carried by the diluent through the detection area through the optical detection element.

23. The detection method of the cell analyzer according to claim 22, wherein: In the reticulocyte detection step, the second syringe drives the reticulocyte detection sample liquid from the first reaction pool to the first sample preparation pipeline, and the first syringe drives the reticulocyte detection sample liquid from the first sample preparation pipeline to the flow chamber; In the white blood cell differential detection step, the second syringe drives the white blood cell differential detection sample liquid to be transported from the second reaction pool to the second sample preparation pipeline, and the first syringe drives the white blood cell differential detection sample liquid to be transported from the second sample preparation pipeline to the flow chamber; The first reaction pool and the second reaction pool are two independent reaction pools, and the first sample preparation pipeline and the second sample preparation pipeline are two independent optical sample preparation pipelines.

24. The detection method of a cell analyzer according to any one of claims 21 to 23, characterized in that: The detection method of the cell analyzer further includes an optical detection cleaning step, an impedance counting detection cleaning step, a hemoglobin detection cleaning step, and a sampling system cleaning step. The optical detection cleaning step includes: driving the diluent from the diluent providing device to the optical sample preparation pipeline and the optical channel reaction pool through the second syringe to clean the optical sample preparation pipeline and the optical channel reaction pool; The impedance counting detection cleaning step includes: driving the diluent from the diluent providing device to the impedance counting detection sample preparation unit through the second syringe to clean the impedance counting detection sample preparation unit; The hemoglobin detection and cleaning step includes: driving the diluent from the diluent supply device to the hemoglobin detection unit through the second syringe to clean the hemoglobin detection unit; The sampling system cleaning step includes: driving the diluent from the diluent supply device to the first syringe and the sampling component through the second syringe to clean the first syringe and the sampling component.

25. The detection method of a cell analyzer according to any one of claims 21 to 23, characterized in that: The impedance counting detection sample preparation unit is integrated with the hemoglobin detection unit, and the impedance counting detection unit includes an impedance counting detection device, a diluent storage tank and a positive pressure power source. The impedance counting detection step includes: preparing the impedance counting detection sample through the hemoglobin detection unit; driving the impedance counting detection sample from the hemoglobin detection unit to the impedance counting sample preparation pipeline through the second syringe; driving the impedance counting detection sample from the impedance counting sample preparation pipeline to the impedance counting detection device through the first syringe, and at the same time driving the diluent from the diluent storage tank to the impedance counting detection device through the positive pressure power source, so that the impedance counting detection sample flows in the impedance counting detection device under the entrainment of the diluent; and performing impedance counting detection on the impedance counting detection sample entrained by the diluent through the impedance counting detection device.

26. The detection method of the cell analyzer according to claim 25, wherein: The detection method of the cell analyzer further includes a reservoir filling step, and the reservoir filling step includes: driving the diluent from the diluent providing device to the diluent reservoir through the second syringe.

27. The detection method of the cell analyzer according to any one of claims 21 to 23, characterized in that: The impedance counting detection sample preparation unit is integrally provided with the hemoglobin detection unit, and the impedance counting detection unit includes an impedance counting detection device, The impedance counting detection step includes: preparing the impedance counting detection sample through the hemoglobin detection unit; driving the impedance counting detection sample from the hemoglobin detection unit to the impedance counting sample preparation pipeline through the second syringe; driving the impedance counting detection sample from the impedance counting sample preparation pipeline to the impedance counting detection device through the first syringe, and at the same time driving the diluent from the diluent providing device to the impedance counting detection device through the second syringe, so that the impedance counting detection sample flows in the impedance counting detection device under the entrainment of the diluent; and performing impedance counting detection on the impedance counting detection sample entrained by the diluent through the impedance counting detection device.

Images