A dry-type fluorescence immunoassay analyzer

Abstract

This invention discloses a dry fluorescence immunoassay analyzer. The base plate is equipped with a pipette tip box, a sample module, an incubation module, a sample dispensing module, and a control module. The sample module is positioned between the pipette tip box and the incubation module, while the sample dispensing module is close to and above the incubation module. The control module is connected to both the incubation module and the sample dispensing module, allowing the sample dispensing module to move relative to the base plate. This invention achieves automatic pipette tip addition, removal, and status detection; automatic removal of waste reagent cards and pipette tips; automatic reading of reagent card information and reagent cup membrane breaking; movement and dynamic light collection; and automatic detection of the sample dispensing liquid level. Utilizing biomedical and chromatographic technologies, it analyzes and processes chromatographic, spectral, and biosensor information for immunoassay and rapid detection. This positioning design enables multi-channel and automated detection, improving its on-site applicability and demonstrating the advantages of automation and simplicity.

Description

Technical Field

[0001] This invention relates to the field of immunoassay analyzer technology, specifically a dry fluorescence immunoassay analyzer. Background Technology

[0002] In recent years, due to environmental and other factors, the emergence and sudden occurrence of infectious diseases have continuously threatened human health. The spread of infectious diseases such as SARS, MERS, influenza, Ebola, Zika virus, yellow fever, and Rift Valley fever has become a global public health issue. With urbanization and increased global trade, environmental changes have exacerbated the risk of transmission of zoonotic infectious diseases, making the prevention and control of infectious diseases an urgent priority. Therefore, conducting rapid and timely testing for infectious diseases is of great significance for the prevention and control of infectious diseases. Simultaneously, rapid testing of human blood samples can effectively and quickly detect existing or potential diseases, which is equally important for effective treatment and early prevention.

[0003] Early immunoassay products were qualitative, with immunoassay projects serving as research subjects, and colloidal gold was used. Colloidal gold products were the star products of this stage. Based on the principle of antigen-antibody binding, theoretically, almost all protein molecules could be labeled. However, their drawbacks included low sensitivity and accuracy, only able to distinguish between negative and positive results. Later, with technological advancements, colored microsphere labeling was adopted. Compared to colloidal gold, colored microsphere labeling offered improved sensitivity and strong repeatability, but its sensitivity remained limited. With further technological development, fluorescent microsphere labeling technology was applied. Fluorescent microsphere labeling provides strong and stable luminescence, high biocompatibility, and is largely unaffected by changes in the external environment, enabling quantitative detection. However, it places higher demands on the product manufacturing process, increasing the complexity of the assay.

[0004] Current analytical instruments require a large number of tedious manual steps for auxiliary detection, making them complex to operate and inefficient. Summary of the Invention

[0005] The purpose of this invention is to overcome the problems mentioned in the background art and to provide a dry fluorescence immunoassay analyzer that is automated, reduces manual steps, and improves efficiency.

[0006] The objective of this invention is mainly achieved through the following technical solutions:

[0007] A dry fluorescence immunoassay analyzer includes a base plate, on which a pipette tip box, a sample module, an incubation module, a sample dispensing module, and a control module are disposed. The sample module is disposed between the pipette tip box and the incubation module, and the sample dispensing module is close to and above the incubation module. The control module is connected to both the incubation module and the sample dispensing module, and the sample dispensing module is movable relative to the base plate. Fluorescence immunoassay, also known as fluorescent antibody technique, is one of the earliest developed labeled immunoassay techniques. It is a technology built upon immunology, biochemistry, and microscopy. In a dry immunofluorescence analyzer, the sample solution dropped onto one end of a membrane is propelled to the other end by the membrane's capillary action (lateral flow based on chromatography). During this movement, the analyte binds to receptors (antigens or antibodies) immobilized in a specific region of the membrane and becomes immobilized. Irrelevant substances cross this region and are separated. The test results are then determined by label detection (qualitative, semi-quantitative, and quantitative). Fluorescence immunoassay analyzers are primarily suitable for blood biochemical analysis and testing in various medical institutions, such as the testing of hormones and infectious diseases. Existing fluorescence immunoassay analyzers require numerous cumbersome manual steps for auxiliary detection, resulting in low efficiency. This solution addresses this by combining modular components as needed. The sample module is positioned between the pipette tip box and the incubation module, while the sample dispensing module is close to and above the incubation module. The control module connects to both the incubation and sample dispensing modules. This design enables multi-channel and automated detection, improving its field applicability and offering advantages in automation and simplicity. It combines traditional dry chemistry techniques, immunochromatography, and optical information and data analysis techniques. Utilizing biomedical and chromatographic technologies, it analyzes and processes chromatographic, spectral, and biosensor information for application in immunoassay and rapid detection.

[0008] Furthermore, the sample module includes a sample holder and a linear guide rail. The linear guide rail is mounted on the base plate, and the sample holder is positioned on and can move along the linear guide rail. Limiting blocks are provided on the linear guide rail. This structural design allows the sample template to be operated as needed, improving work efficiency.

[0009] Furthermore, the incubation module includes an incubation chamber body fixed to a base plate. Several incubation partitions are provided on the incubation chamber body, forming channels for placing reagent strips between adjacent partitions. A temperature heating plate is located at the bottom of the incubation chamber body, directly below the incubation partitions. A waste card removal groove is provided on the side wall of the incubation chamber body, with the top of the groove surface at the same height and connected to the top surface of the incubation chamber body. The width of the waste card removal groove is greater than the length of the reagent strip. All incubation partitions are located on the same side of the incubation chamber body, and the distance between the partitions and the other side of the incubation chamber body is greater than the length of the reagent strip. Currently, in some in vitro diagnostic instruments on the market, the reagent strips are fixed in position after insertion into the instrument, while other components such as sample addition and illumination can only reach designated positions for sample addition and illumination, which complicates the structure of other components. This design creates a channel for placing reagent strips between adjacent incubation partitions, allowing the reagent strips to be adjusted forward and backward. This reduces the travel and complexity of other components, further minimizing the size of the instrument. A heating plate at the bottom of the incubation chamber is also installed to heat the main body, achieving a suitable incubation temperature.

[0010] Furthermore, the sample loading module includes an X-axis drive section, a Y-axis drive section, a sample loading section, and a card ejection / puncture section. The Y-axis drive section is mounted on the X-axis drive section and can reciprocate along the X-axis. Both the sample loading section and the card ejection / puncture section are connected to the Y-axis drive section and can reciprocate relative to the Y-axis drive section in the Z-axis. The sample loading section and the card ejection / puncture section can reciprocate along with the Y-axis drive section in the X-axis and relative to the Y-axis drive section in the Y-axis. The X-axis drive section is fixed to the base plate. Currently, in vitro diagnostic analyzers are inefficient, requiring frequent manual intervention throughout the sample loading and testing process. Moreover, they can only perform one reagent test, consuming significant manpower and resources. To achieve multi-reagent testing and a high degree of automation, they occupy a large space and place high demands on instrument transport. This places a burden on hospitals and clinics using these devices, either in terms of manpower or cost. This solution, however, adopts a three-dimensional movement direction, enabling fully automated sample loading and testing operations within a compact structure. Users only need to place the reagent card and test solution in the designated area, and the sample dispensing device will automatically output the results, avoiding errors caused by manual operation. It is highly efficient and reliable. At the same time, the three-dimensional moving structure design reduces the size, greatly reduces the requirements for installation and use environment, and also reduces costs.

[0011] Furthermore, the X-axis drive section includes a support frame 1, which is fixed to a base plate. A drive mechanism 1 is mounted on the support frame 1 and connected to a transmission mechanism 1. The transmission mechanism 1 is mounted on the support frame 1. The Y-axis drive section is mounted on the support frame 1 and connected to the transmission mechanism 1, and can reciprocate in the X-axis direction with the transmission mechanism 1. A guide rail 2 is mounted on the support frame 1 and fixed to it. The Y-axis drive section is connected to the guide rail 2 and can move along the guide rail 2. This structure provides support and guidance for the X-axis drive section, ensuring stable and accurate X-axis movement.

[0012] Furthermore, the Y-axis drive section includes a Y-axis mounting beam connected to a second guide rail and movable along the second guide rail. A second drive mechanism is mounted on the Y-axis mounting beam, connected to a second transmission mechanism, which is mounted on the Y-axis mounting beam. The sample application section and the card-removing section are mounted on the Y-axis mounting beam and connected to the second transmission mechanism, capable of reciprocating in the Y-axis direction with the second transmission mechanism. A third guide rail is mounted on the Y-axis mounting beam and fixed to it. Both the sample application section and the card-removing section are connected to the third guide rail and movable along it. This structure provides support and guidance for the Y-axis drive section, ensuring its stable and precise Y-axis movement.

[0013] Furthermore, the sample application section includes a sample application arm. A puncture and card ejection mechanism is mounted on the sample application arm and can reciprocate in the Z-axis relative to the arm. The sample application arm is mounted on a Y-axis mounting beam and simultaneously connected to the second transmission mechanism and the third guide rail, and can reciprocate in the Y-axis along with these mechanisms. The sample application arm is equipped with a third drive mechanism and a nozzle mounting block. The nozzle mounting block is connected to the third drive mechanism and can reciprocate in the Z-axis under the action of the third drive mechanism. A nozzle is mounted on the nozzle mounting block. The sample application arm is also equipped with a fourth guide rail and a guide post. The nozzle mounting block is connected to both the fourth guide rail and the guide post and can move along them. This structure provides support and guidance for the sample application section, ensuring its Z-axis movement is in place and stable.

[0014] Furthermore, the card ejection section includes a drive mechanism four and a puncture head mounting block. Both the drive mechanism four and the puncture head mounting block are mounted on the sample feeding arm. The sample feeding arm is equipped with a transmission mechanism three, which is connected to both the drive mechanism four and the puncture head mounting block. The puncture head mounting block can reciprocate in the Z-axis along with the transmission mechanism three, and a puncture head is mounted on the puncture head mounting block. The sample feeding arm is equipped with a guide rail five, and the puncture head mounting block is connected to and can move along the guide rail five. This structure provides support and guidance for the card ejection section, ensuring that the Z-axis movement of the card ejection section is in place and stable.

[0015] In summary, the present invention has the following advantages compared with the prior art:

[0016] (1) The present invention can realize automatic addition, removal and status detection of suction heads;

[0017] (2) This invention can automatically eject waste reagent cards and waste pipette tips;

[0018] (3) This invention can realize automatic reading of reagent card information and reagent cup membrane breaking;

[0019] (4) This invention enables mobile and dynamic light collection;

[0020] (5) This invention can realize automatic detection of the liquid level during sample addition;

[0021] (6) The present invention can realize real-time monitoring, feedback and dynamic adjustment of incubation temperature. Attached Figure Description

[0022] The drawings described herein are used to provide a further understanding of the embodiments of the present invention, constitute a part of this application, and do not constitute a limitation of the embodiments of the present invention. In the drawings:

[0023] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0024] Figure 2 This is a schematic diagram of the sample module.

[0025] Figure 3 This is a schematic diagram of the incubation module.

[0026] Figure 4 This is a schematic diagram of the sample addition module.

[0027] Figure 5 This is a schematic diagram of the sample addition module from another direction.

[0028] Figure 6 This is a schematic diagram of the control module.

[0029] The names corresponding to the reference numerals in the attached figures are:

[0030] 1-Pipette tip box, 2-Sample module, 3-Incubation module, 4-Sample dispensing module, 5-Control module, 6-Base plate, 7-Sample rack, 8-Linear guide rail one, 9-Limiting block, 10-Incubation partition, 11-Reagent card strip, 12-Incubation base plate, 13-Waste card return slot, 14-Position detection plate, 15-Temperature heating plate, 16-Support column, 17-Synchronous pulley and synchronous belt one, 18-X-direction transport code teeth, 19-Drive mechanism two, 20-Guide rail two, 21-X-direction mounting plate, 22-Drive mechanism one, 23-Pipette tip retraction bracket, 24-Pipette tip, 25-Sample dispensing arm, 26-Punch head, 27-Paddle, 28-Guide rail four, 29-Barcode scanner, 30-Drive board one, 31- Y-axis reset optocoupler, 32-drive mechanism three, 33-guide rail three, 34-Y-axis cable protection chain, 35-synchronous pulley and synchronous belt two, 36-Y-axis mounting beam, 37-beam, 38-X-axis reset optocoupler, 39-light-collecting module, 40-drive board two, 41-drive board three, 42-switching power supply, 43-drive board four, 44-mounting plate, 45-sample tube. Detailed Implementation

[0031] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with examples and drawings. The exemplary embodiments of the present invention and their descriptions are only used to explain the present invention and are not intended to limit the present invention. Example

[0032] like Figure 1 As shown, this embodiment includes a base plate 6, on which a pipette tip box 1, a sample module 2, an incubation module 3, a sample dispensing module 4, and a control module 5 are mounted. The sample module 2 is positioned between the pipette tip box 1 and the incubation module 3. The sample dispensing module 4 is located close to and above the incubation module 3. The control module 5 is connected to both the incubation module 3 and the sample dispensing module 4, allowing the sample dispensing module 4 to move relative to the base plate 6. This structural combination achieves component assembly and arrangement, enabling automated operation as needed, reducing manual steps, and improving work efficiency. Example

[0033] like Figure 2 As shown, the sample module 2 in this embodiment includes a sample holder 7 and a linear guide rail 8. The linear guide rail 8 is mounted on the base plate 6, and the sample holder 7 is positioned on and can move along the linear guide rail 8. A limit block 9 is provided on the linear guide rail 8 to limit the movement trajectory and position of the sample holder 7. Using this structure, sample tubes 45 are installed on the sample holder 7, and the sample holder 7 moves via a power mechanism to process samples at different positions. Example

[0034] like Figure 3As shown, the incubation module 3 in this embodiment can place reagent cards, provide a suitable environment for reagent reactions, and cooperate with other devices of the instrument to perform reagent card calibration, removal, etc. It includes an incubation chamber body 12, which has several incubation partitions 10. Adjacent incubation partitions 10 form channels, which are the places where reagent card strips 11 undergo incubation reactions.

[0035] All incubation partitions 10 are positioned on the same side near the main body 12 of the incubation chamber. A heating plate 15 is located at the bottom of the main body 12, directly below the incubation partitions 10. The heating plate 15 is attached to the bottom of the main body 12, heating the main body 12 and the reagent strips 3 on it. A temperature controller is installed on the main body 12, connected to the heating plate 15. The temperature controller monitors and controls the incubation temperature in real time. A position detection plate 14 is located below the heating plate 15, attached to its bottom with one end outside the main body 12. The position detection plate 14 works with other devices to calibrate the position of the reagent strips 11. The main body 12, heating plate 15, and position detection plate 14 are tightly attached together. For easier viewing, the heating plate 15 and position detection plate 14 can be adjusted downwards by a certain distance. Figure 3 As shown.

[0036] The incubation chamber body 12 has a waste card removal groove 13 on its side wall. The top of the groove surface of the waste card removal groove 13 is at the same height as the top surface of the incubation chamber body 12 and is connected to it. The incubation chamber body 12 and the waste card removal groove 13 form a channel for removing waste reagent strips. The width of the waste card removal groove 13 is set to be greater than the length of the reagent strip 11, and the distance between the incubation partition 10 and the other side of the incubation chamber body 12 is greater than the length of the reagent strip 11 to prevent interference.

[0037] Description of the operation process of this invention:

[0038] According to the instrument's operation, the incubation device's operating procedure is as follows:

[0039] 1) Reagent strip position adjustment and calibration: After the reagent strip is placed, other components of the instrument will move the reagent strip back and forth along the channel formed by the incubation chamber body 12 and the incubation partition 10 according to its position. The position detection plate 14 provides real-time feedback on the position of the reagent strip 11, ensuring that the reagent strip 11 reaches the designated position. The back and forth movement of the reagent strip, in coordination with other components, completes various functions.

[0040] 2) Providing an environment for the incubation reaction of the reagent cards: After the reagent cards are positioned correctly, the heating plate 15 at the bottom of the incubation chamber body 12 heats the incubation chamber body 12 and the reagent card strip 14 thereon. In conjunction with the temperature controller installed on the incubation chamber body 12, the incubation temperature is monitored and controlled in real time, providing an environment for the incubation reaction of the reagent cards. During this process, the temperature controller monitors and controls the reaction temperature in real time, maintaining the temperature at the level required for the reagent reaction.

[0041] 3) Reagent Card Removal: After the reagent card strip has finished reacting, other devices in the apparatus will cause the waste reagent card strip to move backward along the channel formed by the incubation chamber body 12 and the incubation partition 10. The waste reagent card strip reaches the rear end of the incubation chamber body 12. Driven by other devices in the apparatus, it moves towards the waste card removal slot 13 and finally slides out of the waste card removal slot 13 to reach the designated waste reagent card placement location.

[0042] This technical solution, along with integrated reagent cards, disposable pipette tips, and other components, allows for the sharing of a single structure for both incubation and reagent card removal, achieving a simple, compact, and versatile design. Real-time temperature monitoring and feedback control of the reaction temperature via a temperature controller provides reliable assurance for test results, enabling real-time temperature monitoring and control. Automatic removal of discarded reagent cards saves significant manpower and resources, preventing human contact with discarded reagent cards and ensuring high safety and reliability. The reagent cards can be adjusted forward and backward, greatly reducing the complexity of other components and resulting in a simple and compact structure. Example

[0043] like Figure 4 and Figure 5 As shown, this embodiment can realize sample information reading, reagent card puncturing, automatic sample addition, card ejection, and detection result acquisition and output using a light-collecting component. It includes an X-axis driving section, a Y-axis driving section, a sample addition section, and a puncture / ejection section, achieving three-dimensional movement. The Y-axis driving section is mounted on the X-axis driving section, and both the sample addition section and the puncture / ejection section are connected to the Y-axis driving section. All three sections can reciprocate along the X-axis, moving as a single unit. The sample addition section and the puncture / ejection section can reciprocate relative to the Y-axis driving section in the Y-axis, again moving as a single unit. The sample addition section and the puncture / ejection section can also reciprocate relative to the Y-axis driving section in the Z-axis, but these movements are performed independently.

[0044] The X-direction drive section includes a support frame 1, which consists of four support columns 16, a crossbeam 37, and an X-direction mounting plate 21. The support columns 16 are arranged vertically and are fixed to the base plate 6. The crossbeam 37 is fixed to two of the support columns 16. The X-direction mounting plate 21 is located between the support columns 16 and the crossbeam 37 and is fixed to both the support columns 16 and the crossbeam 37.

[0045] Drive mechanism 22 is mounted on X-axis mounting plate 21. Drive mechanism 22 serves as an X-axis motor. Drive mechanism 22 is connected to transmission mechanism 1, which is also mounted on X-axis mounting plate 21. Transmission mechanism 1 consists of a synchronous pulley and a synchronous belt 17. The driving pulley and driven pulley of the synchronous pulley and synchronous belt 17 are both mounted on X-axis mounting plate 21 and can rotate around their own axis. The driving pulley is connected to the output shaft of drive mechanism 1. The synchronous belt is sleeved on the outer wall of the driving pulley and driven pulley to form a transmission belt. An X-axis synchronous belt pressure block is provided on the synchronous belt. The Y-axis drive part is mounted on X-axis mounting plate 21 and connected to the synchronous belt through the X-axis synchronous belt pressure block. It can reciprocate in the X-axis direction with transmission mechanism 1.

[0046] To guide the X-axis movement, a second guide rail 20 is installed on the X-axis mounting plate 21. The second guide rail 20 is fixed to the X-axis mounting plate 21 and serves to support the remaining components and allow them to move along the guide rail direction. The Y-axis drive unit is connected to the second guide rail 20 and can move along it. The second guide rail 20 is parallel to the synchronous belt. An X-axis transport gear 18 is installed on the X-axis mounting plate 21, located between the second guide rail 20 and the synchronous belt. An X-axis reset optocoupler 38 and an X-axis reset baffle are also installed on the X-axis mounting plate 21 to achieve the X-axis reset function. Accurate control is achieved through the X-axis transport gear 18, the X-axis reset optocoupler 38, and the X-axis reset baffle.

[0047] Furthermore, the Y-direction drive section includes a Y-direction mounting beam 36, which is connected to and can move along the guide rail 20. A drive mechanism 2 19 is installed on the Y-direction mounting beam 36 as a Y-direction motor. The drive mechanism 2 19 is mounted on a Y-direction motor mounting plate, which is fixed to the Y-direction mounting beam 36. The drive mechanism 2 19 is connected to a transmission mechanism 2, which consists of a synchronous pulley and a synchronous belt 2 35. Its driving pulley is connected to the conveying end of the drive mechanism 2 19. The Y-direction driven pulley is fixed to the Y-direction mounting beam 36 through a Y-direction driven pulley mounting plate. The Y-direction synchronous belt is connected to both the Y-direction driven pulley and the driving pulley to form a transmission belt. The sample feeding section and the puncture and card ejection section are mounted on the Y-direction mounting beam 36 and connected to the Y-direction synchronous belt through the Y-direction synchronous belt pressure block 1 and the Y-direction synchronous belt pressure block 2, and can reciprocate in the Y direction with the transmission mechanism 2.

[0048] To guide Y-axis movement, a guide rail 33 is installed on the Y-axis mounting beam 36. The guide rail 33 is fixed to the Y-axis mounting beam 36 and supports the remaining components, allowing them to move along the guide rail direction. The guide rail 33 is parallel to the Y-axis synchronous belt. Y-axis transport teeth are installed on the Y-axis mounting beam 36, located between the guide rail 33 and the Y-axis synchronous belt. A Y-axis reset baffle, a Y-axis reset optocoupler 31, and a Y-axis reset optocoupler mounting plate are also installed on the Y-axis mounting beam 36. The Y-axis reset optocoupler 31 is mounted on the Y-axis reset optocoupler mounting plate, enabling accurate control of Y-axis movement. Both the sample feeding section and the puncture and ejection section are connected to the guide rail 33 and can move along it. A Y-axis transport drag chain mounting plate and a Y-axis cable protection chain 34 are installed on the Y-axis transport drag chain mounting plate to protect related cables and conduits.

[0049] Furthermore, the sample feeding section includes a sample feeding arm 25, which is preferably an L-shaped structure. The sample feeding arm 25 is mounted on the Y-direction mounting beam 36 and connected to the guide rail 33. Both the Y-direction synchronous belt pressure block 1 and the Y-direction synchronous belt pressure block 2 are fixed to the sample feeding arm 25, allowing the sample feeding arm 25 to reciprocate in the Y direction under the action of the drive mechanism 2. The puncture and card ejection mechanism is mounted on the sample feeding arm 25 and can reciprocate in the Z-direction relative to the sample feeding arm 25. A drive plate 30 is also provided on the sample feeding arm 25 to achieve drive control.

[0050] To achieve Z-axis movement, a drive mechanism 32 and a nozzle mounting block are provided on the sample dispensing arm 25. The drive mechanism 32 acts as a Z-axis motor. The nozzle mounting block is connected to the drive mechanism 32 and can reciprocate in the Z-axis under the action of the drive mechanism 32. A nozzle 24 is mounted on the nozzle mounting block for mounting the pipette tip. The sample dispensing arm 25 is provided with a guide rail 28 and a guide post. The nozzle mounting block is connected to both the guide rail 28 and the guide post and can move along the guide rail 28 and the guide post. A Z-axis optocoupler is also provided on the sample dispensing arm 25 to achieve precise control of Z-axis movement. The sample dispensing section completes the loading of the nozzle tip, sample aspiration, and sample release. It cooperates with the nozzle retraction bracket 23 to complete the unloading of the nozzle tip.

[0051] Furthermore, the puncture and card ejection section includes a drive mechanism four and a puncture head mounting block. Both the drive mechanism four and the puncture head mounting block are mounted on the sample feeding arm 25. A puncture head 26 is mounted on the puncture head mounting block. The drive mechanism four serves as a puncture motor. A transmission mechanism three is provided on the sample feeding arm 25. The transmission mechanism three consists of a puncture drive wheel, a puncture driven wheel, and a puncture timing belt. The puncture drive wheel is mounted on the sample feeding arm 25 and can rotate around its own axis. The puncture driven wheel is mounted on the sample feeding arm 25 through a puncture idler wheel bracket and can rotate around its own axis. The puncture drive wheel is connected to the output end of the drive mechanism four. The puncture timing belt is connected to both the puncture drive wheel and the puncture driven wheel to form a conveyor belt. A puncture timing belt drag block is fixed on the puncture timing belt and is connected to the puncture head mounting block. In this way, the drive mechanism four can drive the puncture timing belt to move, causing the puncture head mounting block and the puncture head 26 to move and achieve the puncture function.

[0052] To guide the puncture action, a guide rail five is installed on the sample dispensing arm 25. The puncture head mounting block is connected to and can move along the guide rail five. The sample dispensing arm 25 is also equipped with a puncture reset optocoupler and a puncture reset baffle to achieve precise control of the puncture displacement. The puncture and card ejection section uses a synchronous pulley and synchronous belt to drive the puncture structure and reagent card alignment in the Z-axis direction, completing the reagent card puncture, reagent card position correction, and ejection of the waste card strip. The sample dispensing arm 25 is equipped with a control board to control the action.

[0053] One of the support columns 16 has a suction head retraction bracket 23 installed on its side wall, and a lever 27 is provided on the sample application arm 25. The lever 27 is connected to the timing belt via a timing belt puncture mounting block. After moving in the X, Y, and Z directions, the lever 27 cooperates with the suction head retraction bracket 23 to complete the action.

[0054] The sample loading arm 25 also includes a light-collecting section, which consists of a light-collecting module 39 and an optical mounting plate. The optical mounting plate is mounted on the sample loading arm 25, and the light-collecting module 39 is mounted on the optical mounting plate. The light-collecting section itself is immovable; it completes light collection, information processing, and output at the corresponding positions as the X and Y axis drive components move. The light-collecting section and the nozzle mounting block are located on the same side of the sample loading arm 25. A position detection mounting plate is also installed on this side of the sample loading arm 25.

[0055] The automatic sampling operation method involved in this plan is as follows:

[0056] 1) Reagent Card Position Calibration: After the reagent card strip is placed, the puncturing and ejecting part of this device moves to the position where the reagent card strip is loaded under the drive of the X and Y direction drive parts. Guide block three moves in the Z direction under the drive of the puncturing motor, puncturing drive wheel, and puncturing timing belt. Under the drive of the Y direction drive part, the reagent card position is calibrated. After calibration, the position detection plate will detect the reagent card position.

[0057] 2) Reagent card information collection: After the reagent card position is corrected, the barcode scanner 29 installed on the sample dispensing arm 25 scans the barcode under the drive of the X and Y direction drive parts. The barcode scanner 29 is installed on the sample dispensing arm 25 through the scanner mounting bracket to collect information about the reagent card and the sample.

[0058] 3) Reagent card strip puncture: After the reagent card strip position is corrected, under the drive of the X and Y direction drive parts, the puncture head 26 moves to the position of the reagent card strip that needs to be punctured. The puncture head 26 moves in the Z direction under the drive of the puncture motor, the puncture drive wheel and the puncture timing belt to puncture the reagent card strip. It is worth noting that the puncture and the reagent card correction share the same set of power and there is no motion interference.

[0059] 4) Sample addition and transfer: Driven by the X and Y direction drive parts, after the pipette tip 24 of the sample addition part moves to the appropriate position, the Z direction motor controls the pipette tip 24 to move in the Z direction to pick up the pipette tip. With the cooperation of the X and Y direction drive parts, it completes the addition and transfer of solution in the sample tube.

[0060] 5) Optical Information Acquisition: After the solution that has completed the reaction is placed in the light-collecting area in the sample addition section, the optical module 39 of the light-collecting section acquires and uploads information under the drive of the X and Y direction drive sections.

[0061] 6) Pipe tip retraction and reagent card ejection: Driven by the X and Y direction drive sections, the pipette tip 24 of the sample dispensing section moves to the appropriate position. With the cooperation of the pipette tip retraction bracket 23, the pipette tip is retracted by the Z direction movement of the Z direction motor. The guide block 3 moves in the Z direction under the drive of the puncture motor, the puncture drive wheel, and the puncture timing belt. With the cooperation of the Y direction drive section, the reagent card strip is retracted along the Y direction.

[0062] This invention utilizes a single structure for both puncturing and removing reagent cards, resulting in a compact and efficient design. After reagents, samples, and consumables are placed inside, the device automatically tests and outputs results, achieving full automation of the process. It automatically records and locates unused disposable pipette tips, precisely controlling sample volume and preventing sample contamination caused by tip reuse, which could affect detection accuracy. The device features a puncture function, and the reagent cards can be sealed, requiring only the corresponding area to be punctured during use. This extends the shelf life of the reagents and facilitates transportation. It can be used with sealed reagents for immediate testing. It automatically removes discarded pipette tips and reagent cards, saving significant manpower and resources, and preventing human contact with discarded reagent cards, ensuring high safety and reliability. Equipped with a serrated detection system, the device's position control is more precise and reliable. By attaching the light-receiving part to the sample dispensing part, results can be quickly generated after the solution reaction, achieving rapid operation. Example

[0063] like Figure 6 As shown, the control module 5 in this embodiment includes a second drive board 40, a third drive board 41, a switching power supply 42, a fourth drive board 43, and a mounting plate 44. These are all existing structures used to achieve automatic control of the components of this invention, reducing manual operation steps and improving work efficiency.

[0064] The principle of this invention is as follows:

[0065] Place the sample to be tested → Enter the test information of the sample to be tested into the testing software → Place the reagent strip that matches the entered information → The sample dispensing module moves the reagent strip to the preset position → The scanner reads the reagent strip information → The sample dispensing module adds a pipette tip → The sample dispensing module aspirates the sample → The puncture head punctures the reagent cup membrane → The sample is discharged into the reagent strip for dilution and mixing → Dispense into the sample dispensing port of the reagent strip → The sample dispensing module removes the pipette tip → The light-collecting module collects light → The sample dispensing module moves the reagent strip and pushes it out.

[0066] The above specific embodiments further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above are merely specific embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A dry fluorescence immunoassay analyzer, comprising a base plate (6), characterized in that: The base plate (6) is provided with a pipette tip box (1), a sample module (2), an incubation module (3), a sample dispensing module (4), and a control module (5). The sample module (2) is located between the pipette tip box (1) and the incubation module (3). The sample dispensing module (4) is close to the incubation module (3) and located above the incubation module (3). The control module (5) is connected to both the incubation module (3) and the sample dispensing module (4). The sample dispensing module (4) can move relative to the base plate (6). The sample module (2) includes a sample holder (7) and a linear guide rail (8). The linear guide rail (8) is installed on the base plate (6). The sample holder (7) is located on the linear guide rail (8) and can move along the linear guide rail (8). 8) Move, and set a limit block (9) on the linear guide rail (8); The incubation module (3) includes an incubation chamber body (12), which is fixed on the base plate (6). Several incubation partitions (10) are set on the incubation chamber body (12), and a channel for placing reagent card strips (11) is formed between adjacent incubation partitions (10). A temperature heating plate (15) is set at the bottom of the incubation chamber body (12), and the temperature heating plate (15) is close to the incubation partitions (10) directly below them; The side wall of the incubation chamber body (12) is provided with a waste card return groove (13). The top of the groove surface of the waste card return groove (13) and the top surface of the incubation chamber body (12) are at the same height and connected. The width of the waste card ejection slot (13) is greater than the length of the reagent card strip (11); the incubation partitions (10) are all located on the same side of the incubation chamber body (12), and the distance between the incubation partitions (10) and the other side of the incubation chamber body (12) is greater than the length of the reagent card strip (11); the sample dispensing module includes an X-axis driving part, a Y-axis driving part, a sample dispensing part, and a puncture and ejection part. The Y-axis driving part is installed on the X-axis driving part and can reciprocate along the X-axis. The sample dispensing part and the puncture and ejection part are both connected to the Y-axis driving part and can reciprocate relative to the Y-axis driving part in the Z-axis direction. The sample dispensing part and the puncture and ejection part can reciprocate along the X-axis with the Y-axis driving part. The sample part and the puncture and card ejection part can reciprocate in the Y direction relative to the Y-direction driving part, and the X-direction driving part and the base plate are fixed; the X-direction driving part includes a support frame one, the support frame one and the base plate (6) are fixed, a driving mechanism one (22) is provided on the support frame one, the driving mechanism one (22) is connected to the transmission mechanism one, the transmission mechanism one is installed on the support frame one, the Y-direction driving part is installed on the support frame one and connected to the transmission mechanism one, and can reciprocate in the X direction with the transmission mechanism one; a guide rail two (20) is provided on the support frame one, the guide rail two (20) is fixed on the support frame one, the Y-direction driving part is connected to the guide rail two (20) and can move along the guide rail two (20);The Y-direction driving section includes a Y-direction mounting beam (36), which is connected to a guide rail (20) and can move along the guide rail (20). A driving mechanism (19) is provided on the Y-direction mounting beam, and a transmission mechanism (2) is connected to the driving mechanism (19). The transmission mechanism (2) is mounted on the Y-direction mounting beam (36). The sample feeding section and the card-removing section are mounted on the Y-direction mounting beam (36) and connected to the transmission mechanism (2), and can move back and forth in the Y direction with the transmission mechanism (2). A guide rail (33) is provided on the Y-direction mounting beam, and the guide rail (33) is fixed on the Y-direction mounting beam (36). The sample feeding section and the card-removing section are both connected to the guide rail (33) and can move along the guide rail (33).

2. The dry fluorescence immunoassay analyzer according to claim 1, characterized in that: The sample feeding part includes a sample feeding arm (25), a puncture and card ejection part is installed on the sample feeding arm (25) and can move back and forth in the Z direction relative to the sample feeding arm (25), the sample feeding arm (25) is installed on the Y direction mounting beam (36) and is simultaneously connected to the second transmission mechanism and the third guide rail (33) and can move back and forth in the Y direction with the second transmission mechanism and the third guide rail (33).

3. The dry fluorescence immunoassay analyzer according to claim 2, characterized in that: The sample feeding arm (25) is provided with a drive mechanism three (32) and a nozzle mounting block. The nozzle mounting block is connected to the drive mechanism three (32) and can move in the Z direction under the action of the drive mechanism three (32). A nozzle (24) is mounted on the nozzle mounting block. The sample feeding arm (25) is provided with a guide rail four (28) and a guide post. The nozzle mounting block is connected to both the guide rail four (28) and the guide post and can move along the guide rail four (28) and the guide post.

4. A dry fluorescence immunoassay analyzer according to claim 2, characterized in that: The puncture and card ejection part includes a drive mechanism four and a puncture head mounting block. Both the drive mechanism four and the puncture head mounting block are mounted on the sample feeding arm (25). The sample feeding arm (25) is provided with a transmission mechanism three, and the transmission mechanism three is connected to both the drive mechanism four and the puncture head mounting block. The puncture head mounting block can move back and forth in the Z direction with the transmission mechanism three. A puncture head (26) is mounted on the puncture head mounting block. The sample feeding arm (25) is provided with a guide rail five. The puncture head mounting block is connected to the guide rail five and can move along the guide rail five.

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