Auxiliary device for detecting physiological indicators of small animals and use method thereof

Abstract

The present invention relates to an auxiliary device for detecting physiological indicators of small animals and a method for using the device. The device comprises a support structure and a device main body supported on the support structure, an elastic tail root fixing component and a tail sleeve tube. The device main body is made of a transparent material, which can facilitate multi-angle observation and recording of the real-time status of the small animal. The device main body is also provided with a bottom opening, which is convenient for the small animal's tail to be naturally extended and for real-time collection of the small animal's excrement. The elastic tail root fixing component fixes the small animal in an open elastic fixing manner, so that the small animal can maintain a quiet and stable state in the device main body, thereby avoiding the small animal from having a stress reaction and free spinning, and further avoiding various equipment lines connected to the small animal from being entangled or disconnected. The tail sleeve tube can be maintained within a certain temperature range, which is convenient for the comfortable placement of the small animal's tail and convenient for experimental sampling operations.

Description

Technical Field

[0001] The present invention relates to the technical field of animal neurological function research, and in particular to an auxiliary device for detecting physiological indicators of small animals and a method for using the device. Background Art

[0002] In animal neurological research, it is often necessary to monitor changes in the animals' physiological indicators. However, during this process, researchers' handling often causes stress reactions in the animals, leading to inaccurate measurements. This is especially true for small animals, such as laboratory mice, which are particularly prone to stress reactions. Furthermore, in neuroscience research, it is often necessary to connect external devices to awake animals for wired stimulation or detection. When animals move around after being connected to wired devices, the external wires can easily become entangled or even disconnected. These entangled wires can also cause stress reactions in the animals, such as tension.

[0003] Currently, there are devices in this field that are used to immobilize small animals when monitoring changes in their physiological indicators, such as restraints and light-controlled real-time blood glucose monitoring devices. Restraints are used to immobilize small animals for intravenous injections and blood sampling. However, these devices completely restrain the animals during experimental manipulation and testing, which clearly causes stress in the animals. Light-controlled real-time blood glucose monitoring devices are used to optogenetically stimulate animals and measure tail vein blood glucose concentrations in real time. Specifically, these light-controlled real-time blood glucose monitoring devices place the animal inside an opaque cylinder with an opening at the bottom, twice the size of the animal. Several small holes are opened around the top and bottom of the cylinder to facilitate light stimulation and tail blood glucose monitoring. In this environment, the animal faces a closed, low-light enclosure, which cannot meet the requirements for real-time observation of its entire state. Furthermore, the animal can move freely inside, making it easy for the relatively light and fragile device cables to become tangled or break when connected to the animal.

[0004] In general, the devices currently used to fix small animals to assist in detecting changes in their physiological indicators have problems such as easily causing stress reactions in the animals and making observation difficult. In addition, due to poor animal fixation, the animals can move and spin freely, causing the equipment wires connected to their bodies to become entangled or broken, making it impossible to ensure the accuracy of detecting changes in the animals' physiological indicators. Summary of the Invention

[0005] Based on this, one purpose of the present invention is to provide an auxiliary device for detecting physiological indicators of small animals and a method of using the device. The device can open and elastically fix the small animal so that the small animal's body is loose and semi-free, and its field of vision is wide. After a short period of adaptation, the small animal can stay in place naturally and quietly, which can prevent the small animal from having a stress response and preventing the small animal from spinning around, and is conducive to accurately detecting changes in physiological indicators of the small animal.

[0006] An auxiliary device for detecting physiological indicators of small animals, comprising a support structure, a device body supported on the support structure, an elastic tail root fixing component, and a tail sleeve tube;

[0007] The device body includes a support portion movably supported on the support structure and a funnel portion extending from the support portion, the funnel portion is used to accommodate the small animal and has a bottom opening at the bottom, the bottom opening is used to allow the tail of the small animal to naturally fall out and facilitate the collection of the small animal's excrement;

[0008] The elastic tail base fixing component is arranged between the bottom opening and the tail sleeve tube, and is used to fix the tail base of the small animal;

[0009] The tail sheath tube is used to fix the back of the small animal's tail, and the temperature of the tail sheath tube is set to be maintained within a certain temperature range to ensure that the small animal's tail can be placed comfortably and facilitate experimental sampling operations.

[0010] In one embodiment of the present invention, the device body is made of a transparent material and both the inner and outer surfaces are smooth to prevent the small animals from being blocked, making it easier to observe and record the real-time status of the small animals.

[0011] In one embodiment of the present invention, the elastic tail base fixing assembly includes a sticky tape, a clip, an elastic member and a tail base fixing ring, wherein the sticky tape is used to stick to the tail base extending from the bottom opening, the clip is used to clamp the clampable part of the sticky tape, and the elastic member is used to bind and fix the clip to the tail base fixing ring, so as to keep the small animal within an autonomous elastic range, and the head of the small animal can face the fixed side of the funnel part without rotating, thereby keeping various connections on the small animal quietly and stably connected.

[0012] In one embodiment of the present invention, the tail base fixing ring has two fixing ears, and after the elastic member binds and fixes the clip, both ends are fixed to the corresponding fixing ears respectively.

[0013] In one embodiment of the present invention, the support structure includes a support base, an L-shaped bracket connected to the support base, and a support ring connected to the L-shaped bracket. The support portion of the device body is a cylindrical structure and an extension flange is extended from the top. The support portion can be movably mounted in the support ring and can form a state in which the bottom surface of the extension flange and the top surface of the support ring are supported against each other, so that the state of the device body can be adjusted by lifting the support portion, thereby inducing small animals to actively climb and extend their tails from the bottom opening of the funnel.

[0014] In one embodiment of the present invention, the support portion has a top opening, so that a connection line of an external detection device can be introduced from the top opening into the funnel portion and connected to the small animal.

[0015] In one embodiment of the present invention, the support structure further includes a first support column connecting the support base and the tail base fixing ring and a second support column connecting the support base and the tail sleeve tube.

[0016] A method for using an auxiliary device for detecting physiological indicators of small animals, comprising the steps of:

[0017] S1. Place the small animal in the funnel portion of the assistive device so that the tail of the small animal naturally extends out of the funnel portion;

[0018] S2. Using adhesive tape to adhere to the base of the small animal's tail, using a clip to clamp the clampable portion of the adhesive tape, using an elastic member to bind the clip, and fixing both ends of the elastic member to the tail base fixing ring;

[0019] S3, inserting a tail cuff into the rear of the animal's tail and maintaining the tail cuff at a preset temperature; and

[0020] S4. Connect the connecting line of the detection equipment to the connecting end of the small animal.

[0021] In one embodiment of the present invention, step S1 includes the following steps:

[0022] placing small animals into the infundibulum for multiple times to allow them to move freely so as to familiarize themselves with the environment of the infundibulum; and

[0023] The device body is tilted and lifted to enable the small animal to actively climb and induce it to extend its tail through the bottom opening of the funnel.

[0024] In one embodiment of the present invention, the method for using the auxiliary device for detecting physiological indicators of small animals also includes the following steps: S5, after the small animal adapts to the connection line of the detection equipment, any one or more experimental operations including optogenetic stimulation, electrical stimulation, catheter drug administration, catheter body fluid collection, tail blood collection, and myoelectric electromyography collection are performed on the small animal.

[0025] The auxiliary device for detecting physiological indicators of small animals of the present invention uses an open elastic fixation method to fix the small animals, so that the small animals can naturally and elastically maintain a quiet and stable state, thereby preventing the small animals from having stress reactions and spinning freely, and further preventing various equipment wires connected to the small animals from being entangled or disconnected. In particular, lightweight filament wires will not be bitten off by the small animals or torn off due to the spinning and entanglement of the small animals, which can reduce the foreign body sensation on the small animals and smoothly connect various precision detection equipment.

[0026] The device body of the auxiliary device for detecting physiological indicators of small animals of the present invention is made of transparent material, which can facilitate multi-angle naked eye observation or use a camera to record the real-time status of the small animal in the device body, meeting the need for real-time observation of the entire status.

[0027] The device body of the auxiliary device for detecting physiological indicators of small animals of the present invention is provided with the bottom opening, which not only facilitates the natural extension of the tail of the small animal, but also facilitates the real-time collection of the excrement of the small animal.

[0028] The tail sheath tube used in the auxiliary device for detecting physiological indicators of small animals of the present invention can be maintained within a certain temperature range, such as between 25°C and 37°C, that is, maintained within a temperature range that is more comfortable for small animals, so that the tail of the small animal can be placed comfortably and is convenient for experimental operations, such as blood sampling from the tail of the small animal.

[0029] Further objects and advantages of the present invention will be fully apparent from an understanding of the following description and accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a structural schematic diagram of the auxiliary device for detecting physiological indicators of small animals according to a preferred embodiment of the present invention.

[0031] Figure 2 This is a flowchart of a method for using the auxiliary device for detecting physiological indicators of small animals according to the above-mentioned selected embodiment of the present invention.

[0032] Explanation of the accompanying drawings: support structure 10; support base 11; L-shaped bracket 12; support ring 13; first support column 14; second support column 15; device body 20; support portion 21; extension flange 211; top opening 212; funnel portion 22; bottom opening 221; elastic tail base fixing assembly 30; adhesive tape 31; clip 32; elastic member 33; tail base fixing ring 34; fixing ear 341; tail sleeve tube 40. DETAILED DESCRIPTION

[0033] The following description is intended to disclose the present invention and enable those skilled in the art to implement the present invention. The preferred embodiments described below are provided for illustrative purposes only, and those skilled in the art will readily appreciate other obvious variations. The basic principles of the present invention defined in the following description may be applied to other embodiments, variations, improvements, equivalents, and other technical solutions that do not depart from the spirit and scope of the present invention.

[0034] Those skilled in the art should understand that, in the disclosure of the present invention, the terms "vertical", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like to indicate orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the above terms should not be understood as limiting the present invention.

[0035] It is to be understood that the term "one" should be understood as "at least one" or "one or more", that is, in one embodiment, the number of an element may be one, while in another embodiment, the number of the elements may be multiple, and the term "one" should not be understood as a limitation on the quantity.

[0036] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections, or mutual communication; direct connections or indirect connections through an intermediate medium; and internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0037] The present invention provides an open elastic auxiliary device for fixing small animals, so that the small animals can have a loose and semi-free body and a wide field of vision. After a short period of adaptation, they can stay in place naturally and quietly, thereby preventing the small animals from having stress reactions and spinning freely, ensuring accurate detection of changes in physiological indicators of small animals, and providing accurate experimental basis for animal neurological function research.

[0038] like Figure 1As shown, the specific structure of an auxiliary device for detecting physiological indicators of small animals according to the present invention is explained. The auxiliary device for detecting physiological indicators of small animals includes a support structure 10 and a device body 20 supported on the support structure 10, an elastic tail base fixing component 30, and a tail sleeve tube 40; wherein the device body 20 includes a support portion 21 movably supported on the support structure 10 and a funnel portion 22 extending from the support portion 21, the funnel portion 22 is used to accommodate the small animal and has a bottom opening 221 at the bottom, the bottom opening 221 is used to allow the small animal's tail to fall naturally and extend out and facilitate the collection of the small animal's excrement; wherein the elastic tail base fixing component 30 is arranged between the bottom opening 221 and the tail sleeve tube 40, and is used to fix the base of the small animal's tail; wherein the tail sleeve tube 40 is used to fix the back of the small animal's tail, and the temperature of the tail sleeve tube 40 is set to be maintained within a certain temperature range to ensure that the small animal's tail can be placed comfortably and facilitate experimental sampling operations.

[0039] In particular, the device body 20 is made of transparent material, and both the inner and outer surfaces are glossy to prevent the small animal from being obscured. Most of the body of the small animal can be seen with the naked eye from multiple angles, thereby facilitating multi-angle naked eye observation or using a camera to record the real-time status of the small animal in the device body 20, meeting the need for real-time observation of the entire status.

[0040] Preferably, the device body 20 is made of acrylic.

[0041] It is worth mentioning that the tail sleeve tube 40 can be connected to an external heating device or covered with an insulation bag to ensure that its temperature can be maintained within a certain temperature range. The present invention does not limit the specific temperature maintenance method of the tail sleeve tube 40.

[0042] In addition, it is worth mentioning that the temperature of the tail sheath tube 40 is preferably maintained at 25°C to 37°C, which is close to the body temperature of the small animal and is kept within a temperature range that is more comfortable for the small animal. This allows the small animal's tail to be placed comfortably and facilitates experimental sampling operations, such as blood sampling from the small animal's tail.

[0043] Furthermore, the elastic tail base fixing assembly 30 includes a sticky tape 31, a clip 32, an elastic member 33 and a tail base fixing ring 34, wherein the sticky tape 31 is used to stick to the tail base extending from the bottom opening 221, the clip 32 is used to clamp the clampable part of the sticky tape 31, and the elastic member 33 is used to bind and fix the clip 32 to the tail base fixing ring 34, so as to keep the small animal within an autonomous elastic range, and the head of the small animal can face the fixed side of the funnel portion 22 without rotating, thereby keeping various connections on the small animal quietly and stably connected.

[0044] It is worth mentioning that the adhesive tape 31 is preferably a medical adhesive tape, the clampable portion of the adhesive tape 31 is the portion other than the portion that sticks to the base of the animal's tail, and the elastic member 33 is preferably a rubber band.

[0045] Furthermore, the tail base fixing ring 34 has two fixing ears 341 , and after the elastic member 33 binds and fixes the clip 32 , both ends are fixed to the corresponding fixing ears 341 .

[0046] It is understandable that, by elastically fixing only the tail of the small animal through the elastic tail base fixing assembly 30, the small animal can be in a loose, semi-free state. Moreover, since the device body 20 is a transparent device, the small animal has a wide field of vision. After a short period of semi-free movement, the small animal can adapt and naturally stay in place quietly, naturally and elastically maintaining a quiet and stable state to avoid a stress response. At the same time, because the base of the small animal's tail is fixed to the tail base fixing ring 34 via the clip 32 and the elastic member 33, the small animal can be kept within an autonomous elastic range with its head facing the fixed side of the funnel without spinning, thereby maintaining a quiet and stable connection for various detection equipment.

[0047] That is to say, the auxiliary device for detecting physiological indicators of small animals adopts an open elastic fixation method to fix the small animals, so that the small animals can naturally and elastically maintain a quiet and stable state, thereby preventing the small animals from having stress reactions and spinning freely, and thus preventing various equipment wires connected to the small animals from being entangled or disconnected. In particular, lightweight filament wires will not be bitten off by the small animals or torn off due to the spinning and entanglement of the small animals, which can reduce the foreign body sensation on the small animals and smoothly connect various precision detection equipment.

[0048] It is worth mentioning that the support structure 10 includes a support base 11, an L-shaped bracket 12 connected to the support base 11, and a support ring 13 connected to the L-shaped bracket 12. The support portion 21 of the device body 20 is a cylindrical structure and an extension flange 211 is formed on the top. The support portion 21 can be movably arranged in the support ring 13 and can form a state in which the bottom surface of the extension flange 211 is in contact with the top surface of the support ring 13, so that the device body 20 can be movably supported on the support structure 10.

[0049] It can be understood that since the device body 20 can be movably supported on the L-shaped bracket 12, when the tail of the small animal is coiled inside the funnel, the state of the device body 20 can be adjusted by lifting the support part 21. For example, the device body 20 can be lifted at an angle, so as to facilitate inducing the small animal to actively climb and extend its tail from the bottom opening 221 of the funnel part 22.

[0050] In other words, by setting the device body 20 to be movably supported on the support structure 10, the posture of the small animal inside the device body 20 can be adjusted by adjusting the posture of the device body 20 to meet the needs of different experiments, while avoiding the small animal's stress response caused by taking adjustment methods such as direct grasping.

[0051] It is worth mentioning that the support portion 21 has a top opening 212 so that the connection line of the external detection equipment can be introduced into the funnel portion 22 from the top opening 212 and connected to the small animal.

[0052] Furthermore, the support structure 10 further includes a first support column 14 connecting the support base 11 and the tail base fixing ring 34 and a second support column 15 connecting the support base 11 and the tail sleeve tube 40 .

[0053] It is worth mentioning that the tail base fixing ring 34 , the tail sleeve tube 40 and the support structure 10 are preferably made of stainless steel.

[0054] In addition, it is worth mentioning that the auxiliary device of the present invention is used to assist in the detection of small animals such as mice, but may also be other small animals. The present invention does not limit the specific type of experimental small animals.

[0055] In this embodiment of the present invention, the specific dimensions of the auxiliary device for detecting physiological indicators of small animals are as follows: the support base 11 is a rectangular structure with a length of 50 cm, a width of 30 cm, and a height of 8 cm. The diameter of the top opening 212 of the device body 20 is 13 cm, the depth of the support part 21 is 6.5 cm, the depth of the funnel part 22 is 12.5 cm, and the funnel part 22 is fan-shaped when unfolded, with a radius of 15 cm. The diameter of the bottom opening 221 is 1 cm, and the distance between the bottom opening 221 and the support base 11 is 15 cm.

[0056] It should be understood that, according to different experimental requirements, the auxiliary device for detecting physiological indicators of small animals may have other sizes, and the present invention is not limited thereto.

[0057] In this embodiment of the present invention, the specific steps for using the auxiliary device for detecting physiological indicators of small animals are as follows:

[0058] First, the small animal needs to move freely in the funnel portion 22 of the device body 20 to become familiar with the environment of the funnel portion 22 for three consecutive days, about one hour each day.

[0059] It is worth mentioning that after the small animals become familiar with the device body 20, they will quietly climb and stay at the bottom of the funnel 22. Some will actively extend their tails out of the opening 221 at the bottom of the funnel, and some will coil their tails inside the funnel. The device body 20 can be lifted out separately and slightly tilted to allow the small animals to actively climb and induce their tails to extend out of the opening 221 at the bottom of the funnel.

[0060] Next, use a small piece of medical adhesive tape to stick to the base of the tail, leaving an extra handle for the small clip to clamp. The small clip is tied with an elastic member 33 and the two ends of the elastic member 33 are fixed to the tail base fixing ring 34.

[0061] Next, after the tail passes through the tail base fixing ring 34, the tail is inserted into the tail sleeve tube 40, and a small insulation bag is placed on the tail sleeve tube 40 to maintain a certain temperature, preferably close to the body temperature of the small animal.

[0062] Finally, connect the equipment that needs to be connected to the small animal to the small animal connection end. After the small animal has adapted for 40 minutes, specific experimental operations can be carried out, such as: optogenetic stimulation, electrical stimulation, catheter drug delivery, catheter fluid collection, tail blood collection, myoelectric electromyography collection, etc.

[0063] That is to say, if Figure 2 As shown, the present invention further provides a method for using the auxiliary device for detecting physiological indicators of small animals, comprising the steps of:

[0064] S1. Place the small animal in the funnel portion 22 of the auxiliary device so that the tail of the small animal naturally extends out of the funnel portion 22;

[0065] S2. Use adhesive tape 31 to stick to the base of the small animal's tail, and use a clip to clamp the clampable part of the adhesive tape 31. Use an elastic member 33 to bind the clip 32 and fix the two ends of the elastic member 33 to the tail base fixing ring 34.

[0066] S3, inserting the rear part of the small animal's tail into the tail sheath tube 40, and maintaining the tail sheath tube 40 at a preset temperature; and

[0067] S4. Connect the connecting line of the detection equipment to the connecting end of the small animal.

[0068] Specifically, step S1 includes the following steps:

[0069] placing small animals into the infundibulum 22 for multiple times to allow them to move freely so as to familiarize themselves with the environment of the infundibulum 22; and

[0070] The device body 20 is tilted and lifted to enable the small animal to actively climb and induce it to extend its tail through the bottom opening 221 of the funnel portion 22 .

[0071] Furthermore, the method for using the auxiliary device for detecting physiological indicators of small animals also includes the step: S5, after the small animal adapts to the connection line of the detection equipment, the small animal is subjected to any one or more experimental operations including optogenetic stimulation, electrical stimulation, catheter drug administration, catheter body fluid collection, tail blood collection, and myoelectric electromyography collection.

[0072] In general, the auxiliary device for detecting physiological indicators of small animals has the following advantages:

[0073] (1) The small animal is fixed with an open elastic fixation method, so that the small animal can naturally elastically remain in a quiet and stable state, thereby preventing the small animal from having a stress reaction and spinning freely;

[0074] (2) The wires of various devices connected to small animals will not be entangled or disconnected, especially lightweight filament wires will not be entangled, torn, or bitten by small animals, which can reduce the foreign body sensation on small animals and smoothly connect various precision equipment;

[0075] (3) The device body 20 is made of a transparent material so that the real-time status of the small animal can be observed and recorded from multiple angles by the naked eye or an ordinary camera;

[0076] (4) The tail of the small animal can be elastically and stably placed in the tail sleeve tube 40 maintained within a certain temperature range, and the tail can be placed comfortably and conveniently for experimental sampling operations;

[0077] (5) The bottom opening 221 of the device body 20 can facilitate the natural fall and extension of the small animal's tail, and can also collect the small animal's excrement in real time.

[0078] The technical features of the above embodiments can be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0079] The above embodiments merely represent preferred embodiments of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make various modifications and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. An auxiliary device for detecting physiological indicators of small animals, characterized in that: It includes a support structure, a device body supported on the support structure, an elastic tail root fixing component and a tail sleeve tube; The device body includes a support portion movably supported on the support structure and a funnel portion extending from the support portion, the funnel portion is used to accommodate the small animal and has a bottom opening at the bottom, the bottom opening is used to allow the tail of the small animal to naturally fall out and facilitate the collection of the small animal's excrement; The elastic tail base fixing component is arranged between the bottom opening and the tail sleeve tube, and is used to fix the tail base of the small animal; The tail sheath is used to fix the back of the small animal's tail, and the temperature of the tail sheath is set to be maintained within a certain temperature range to ensure that the small animal's tail can be placed comfortably and facilitate experimental sampling operations; The elastic tail base fixing assembly includes a sticky tape, a clip, an elastic member and a tail base fixing ring, wherein the sticky tape is used to stick to the tail base extending from the bottom opening, the clip is used to clamp the clampable part of the sticky tape, and the elastic member is used to bind and fix the clip to the tail base fixing ring.

2. The auxiliary device for detecting physiological indicators of small animals according to claim 1, characterized in that: The device body is made of transparent material and has smooth inner and outer surfaces.

3. The auxiliary device for detecting physiological indicators of small animals according to claim 1, characterized in that: The tail base fixing ring has two fixing ears. After the elastic member binds and fixes the clip, the two ends are respectively fixed to the corresponding fixing ears.

4. The auxiliary device for detecting physiological indicators of small animals according to claim 1, characterized in that: The supporting structure includes a supporting base, an L-shaped bracket connected to the supporting base, and a supporting ring connected to the L-shaped bracket. The supporting portion of the device body is a cylindrical structure and an extended flange is extended from the top. The supporting portion can be movably mounted in the supporting ring and can form a state in which the bottom surface of the extended flange is in contact with the top surface of the supporting ring.

5. The auxiliary device for detecting physiological indicators of small animals according to claim 4, characterized in that: The support portion has a top opening, so that a connection line of an external detection device can be introduced into the funnel portion from the top opening and connected to the small animal.

6. The auxiliary device for detecting physiological indicators of small animals according to claim 4, characterized in that: The support structure further includes a first support column connecting the support base and the tail base fixing ring and a second support column connecting the support base and the tail sleeve tube.

7. A method for using the auxiliary device for detecting physiological indicators of small animals as described in any one of claims 1 to 6, characterized in that: Including steps: S1. Place the small animal in the funnel portion of the assistive device so that the tail of the small animal naturally extends out of the funnel portion; S2. Using adhesive tape to adhere to the base of the small animal's tail, using a clip to clamp the clampable portion of the adhesive tape, using an elastic member to bind the clip, and fixing both ends of the elastic member to the tail base fixing ring; S3, inserting a tail cuff into the rear of the animal's tail and maintaining the tail cuff at a preset temperature; and S4. Connect the connecting line of the detection equipment to the connecting end of the small animal.

8. The method for using the auxiliary device for detecting physiological indicators of small animals according to claim 7, characterized in that: The step S1 comprises the steps of: placing small animals into the infundibulum for multiple times to allow them to move freely so as to familiarize themselves with the environment of the infundibulum; and The device body is tilted and lifted to enable the small animal to actively climb and induce it to extend its tail through the bottom opening of the funnel.

9. The method for using the auxiliary device for detecting physiological indicators of small animals according to claim 7, characterized in that: The method further includes the following steps: S5, after the small animal has adapted to the connection line of the detection device, any one or more experimental operations including optogenetic stimulation, electrical stimulation, catheter drug administration, catheter fluid collection, tail blood collection, and myoelectric electromyography collection are performed on the small animal.

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