A protein blotting transfer cooling device

Abstract

The present invention belongs to the technical field of experimental equipment, and relates to a protein blot transfer cooling device, including: a box body, a cooling cavity; a cover body, a heat exchange box, a liquid inlet pipe, and a liquid outlet pipe; a cooling component, which is arranged inside the box body and is respectively connected to one end of the liquid inlet pipe and one end of the liquid outlet pipe, and is used to cool down the transfer box placed inside the box body; a heat exchange component, which is arranged inside the heat exchange box and is respectively connected to the other end of the liquid inlet pipe and the other end of the liquid outlet pipe, and is used to provide cooling liquid to the liquid inlet pipe and cool down the cooling liquid refluxed from the liquid outlet pipe. The present invention, through the arrangement of the cooling component and the heat exchange component, enables the temperature generated inside the box body during the transfer process due to the transfer box to be quickly heat exchanged through the cooling component, thereby quickly reducing the temperature inside the box body to a temperature range suitable for the transfer experiment, and at the same time, the heat generated during the transfer box experiment is taken away by the cooling liquid, providing a suitable experimental temperature for the transfer experiment, and improving the efficiency of the transfer.

Description

Technical Field

[0001] The invention belongs to the technical field of experimental equipment and relates to a protein blot transfer cooling device. Background Art

[0002] Western Blot (WB) is a common experimental method for detecting protein expression levels. One of the important steps is membrane transfer, which can be performed by dry transfer, semi-dry transfer, or wet transfer. Among them, wet transfer is the most commonly used and has the highest efficiency. During the wet transfer process, the membrane transfer instrument will generate a large amount of heat due to the action of the electric current during operation, and excessively high temperature will reduce the efficiency of membrane transfer. Therefore, a common practice is to fill the ice box in the electrophoresis box with ice, and then add an ice-water mixture to the periphery of the membrane transfer box before starting the membrane transfer. Ice cubes need to be prepared during the experiment, and the ice-adding and preparation process is cumbersome, resulting in slow progress of the experiment and relatively low work efficiency. Summary of the invention

[0003] The present invention aims to provide a protein blot transfer cooling device to solve the technical problem that the existing protein blot transfer has a cumbersome cooling process by adding ice when the wet transfer method is adopted, thus resulting in low working efficiency.

[0004] To achieve the above-mentioned purpose, the specific technical scheme of a protein blot transfer cooling device of the present invention is as follows:

[0005] A protein blotting transfer cooling device, comprising:

[0006] The box body has an open structure at the top and a hollow structure on the side wall to form a cooling cavity;

[0007] The cover body is arranged at the upper opening of the box body and is buckled at the opening of the box body;

[0008] The heat exchange box is arranged on the side of the box body and is connected to the box body through a liquid inlet pipe and a liquid outlet pipe;

[0009] A cooling component is arranged inside the box body and is connected to one end of the liquid inlet pipe and one end of the liquid outlet pipe respectively, and is used to cool the transfer box placed inside the box body;

[0010] The heat exchange component is arranged inside the heat exchange box and is connected to the other end of the liquid inlet pipe and the other end of the liquid outlet pipe respectively, and is used to provide cooling liquid to the liquid inlet pipe and cool the cooling liquid returning from the liquid outlet pipe.

[0011] The present invention is also characterized in that:

[0012] The cooling assembly includes a cooling tube, which is arranged inside the cooling cavity. The cooling tube is arranged in an S shape around the side wall of the box body. A plurality of heat exchange fins are evenly arranged between the S-shaped cooling tubes. The plurality of heat exchange fins are arranged around the side wall of the box body. One end of each heat exchange fin is fixedly connected to an inner wall on one side of the cooling cavity, and the other end of each heat exchange fin is located inside the box body after passing through the inner wall on the other side of the cooling cavity. The upper end of the cooling tube is connected to one end of the liquid outlet pipe, and the lower end of the cooling tube is connected to one end of the liquid inlet pipe.

[0013] The heat exchange component includes a refrigerator, which is arranged inside the heat exchange box. A plurality of refrigeration plates and an agitator are arranged inside the refrigerator. The other end of the liquid inlet pipe passes through the side wall of the heat exchange box and is connected to the side of the refrigerator. The other end of the liquid outlet pipe passes through the other side wall of the heat exchange box and is connected to the other side of the refrigerator. The refrigerator is respectively connected with the liquid outlet pipe and the liquid inlet pipe. A liquid injection pipe is vertically arranged on the upper part of the heat exchange box. The lower end of the liquid injection pipe passes through the top wall of the heat exchange box and is connected to the refrigerator. The liquid injection pipe is connected to the refrigerator, and a water pump is arranged on the liquid inlet pipe near the refrigeration position.

[0014] The portion of the liquid outlet pipe located inside the heat exchanger box is S-shaped, a first fan is arranged on the top wall of the heat exchanger box near the liquid outlet pipe, a plurality of air inlet holes are opened on the top wall of the heat exchanger box near the first fan, and a plurality of air outlet holes are arranged on the lower portion of the side wall of the heat exchanger box near the liquid outlet pipe.

[0015] A shielding sheet is arranged on the outer wall of the heat exchange box near the upper part of each air outlet, and the shielding sheet is inclined downward.

[0016] A controller is arranged at the upper part of the heat exchange box, and a plurality of temperature sensors are arranged at the upper part and the lower part of the inner wall of the box body respectively. The controller is electrically connected to the water pump, the first fan and the temperature sensor respectively.

[0017] A plurality of protrusions are arranged on the bottom of the box body.

[0018] A second fan is arranged at the bottom of the box body, and the second fan is arranged between a plurality of protrusions. A third fan is arranged at the lower part of the cover body, and both the second fan and the third fan are electrically connected to the controller.

[0019] The box body and the cover body are both made of heat-insulating materials.

[0020] The protein blotting transfer cooling device of the present invention has the following advantages:

[0021] First, by setting the cooling component and the heat exchange component, the temperature inside the box body generated by the film transfer box during the mold transfer process can be quickly heat exchanged through the cooling component, so as to quickly reduce the temperature inside the box body to a temperature range suitable for the mold transfer experiment. At the same time, the heat generated during the film transfer box experiment is taken away by the coolant, providing a suitable experimental temperature for the mold transfer experiment and improving the efficiency of the mold transfer. At the same time, the heat exchange component can quickly cool the cooling liquid with a high temperature, so that the cooling liquid can be recycled, saving the experimental cost;

[0022] Second, by setting up the second and third fans, the air inside the box can generate convection, which accelerates the heat exchange rate of the heat exchanger and quickly reduces the temperature inside the box, thus providing a guarantee for the mold rotation experiment.

[0023] Third, by cooperating with the first fan and the S-shaped liquid outlet pipe, the returning coolant is cooled for the first time, so that it is easy to cool down quickly after entering the refrigerator, thereby improving the cooling rate of the coolant;

[0024] Fourth, by coordinating the controller and the temperature sensor, the temperature inside the box is monitored in real time, so that the temperature inside the box can always be maintained in a temperature range suitable for the mold transfer experiment, further improving the efficiency of the mold transfer. At the same time, the box body and the cover are made of thermal insulation materials to avoid the external environment from affecting the temperature inside the box, ensuring that the temperature inside the box remains stable. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the overall structure of a protein blotting transfer cooling device of the present invention;

[0026] Figure 2 It is a schematic diagram of the front view structure of a box body and a cover body in a protein blotting transfer cooling device of the present invention;

[0027] Figure 3 It is a schematic diagram of the unfolded structure of cooling tubes and heat exchange fins in a protein blot transfer cooling device of the present invention;

[0028] Figure 4 This is a schematic diagram of a top view of a box body in a protein blotting transfer cooling device of the present invention;

[0029] Figure 5 This is a schematic diagram of the front view structure of a heat exchange box in a protein blotting transfer cooling device of the present invention;

[0030] Reference numerals:

[0031] 1. Box body; 2. Cover body; 3. Cooling pipe; 4. Heat exchange plate; 5. Liquid inlet pipe; 6. Liquid outlet pipe; 7. Heat exchange box; 8. Water pump; 9. Refrigeration machine; 10. Refrigeration plate; 11. Agitator; 12. Liquid injection pipe; 13. Shielding plate; 14. First fan; 15. Second fan; 16. Temperature sensor; 17. Controller; 18. Air inlet hole; 19. Air outlet hole; 20. Cooling chamber; 21. Protrusion; 22. Third fan. DETAILED DESCRIPTION

[0032] In order to better understand the purpose, structure and function of the present invention, a protein blotting transfer cooling device of the present invention is further described in detail below in conjunction with the accompanying drawings.

[0033] like Figure 1 As shown, the present invention is a protein blot transfer cooling device, including a box body 1, the upper part of the box body 1 is an open structure, the side wall is a hollow structure to form a cooling chamber 20, the upper opening of the box body 1 is provided with a cover body 2, the cover body 2 is buckled at the opening of the box body 1 and a sealing structure is provided at the buckling part, the box body 1 and the cover body 2 are both made of heat-insulating materials to avoid the external environment from affecting the temperature inside the box body 1, ensuring that the temperature inside the box body 1 is maintained within a stable range, so that the transfer box placed therein can normally perform the transfer experiment, and the side of the box body 1 is provided with A heat exchange box 7 is connected to the box body 1 through a liquid inlet pipe 5 and a liquid outlet pipe 6. A cooling component is arranged inside the box body 1. The cooling component is respectively connected to one end of the liquid inlet pipe 5 and one end of the liquid outlet pipe 6, and is used to cool the transfer box placed inside the box body 1 so that the temperature of the transfer box is always maintained within a low temperature range suitable for the reaction. A heat exchange component is arranged inside the heat exchange box 7. The heat exchange component is respectively connected to the other end of the liquid inlet pipe 5 and the other end of the liquid outlet pipe 6, and is used to provide cooling liquid to the liquid inlet pipe 5 and cool the cooling liquid refluxed from the liquid outlet pipe 6.

[0034] like Figure 2 , 3 As shown in Figure 4, the cooling assembly includes a cooling tube 3, which is arranged inside the cooling cavity 20. The cooling tube 3 is arranged in an S shape around the side wall of the box body 1. A plurality of heat exchange plates 4 are evenly arranged between the S-shaped cooling tubes 3. The plurality of heat exchange plates 4 are arranged around the side wall of the box body 1. One end of each heat exchange plate 4 is fixedly connected to an inner wall on one side of the cooling cavity 20, and the other end of each heat exchange plate 4 is located inside the box body 1 after passing through the inner wall on the other side of the cooling cavity 20. The upper end of the cooling tube 3 is connected to one end of the liquid outlet pipe 6, and the lower end of the cooling tube 3 is connected to one end of the liquid inlet pipe 5. The cooling tube 3 is heat exchanged with the inside of the box body 1 through the heat exchange plate 4, and the heat generated during the membrane transfer box experiment is taken away by the coolant.

[0035] like Figure 5As shown, the heat exchange assembly includes a refrigerator 9, which is arranged inside the heat exchange box 7. A plurality of refrigeration fins 10 and a stirrer 11 are arranged inside the refrigerator 9. The refrigeration fins 10 are used to cool the refluxed coolant, and the stirrer 11 is used to stir the coolant to balance the coolant temperature inside the refrigerator 9. The other end of the liquid inlet pipe 5 passes through the side wall of the heat exchange box 7 and is connected to the side of the refrigerator 9. The other end of the liquid outlet pipe 6 passes through the other side wall of the heat exchange box 7 and is connected to the other side of the refrigerator 9. The refrigerator 9 is connected with the liquid outlet pipe 6 and the liquid inlet pipe 5 respectively. The coolant enters the liquid inlet pipe 5 after being cooled in the refrigerator 9. After circulating in the cooling pipe 3 for one circle, it circulates into the refrigerator 9 again through the liquid outlet pipe 6. A liquid injection pipe 12 is vertically arranged on the upper part of the heat exchange box 7. The lower end of the liquid injection pipe 12 passes through the top wall of the heat exchange box 7 and is connected to the refrigerator 9. The liquid injection pipe 12 is connected with the refrigerator 9. Coolant is added to the refrigerator 9 through the liquid injection pipe 12. A water pump 8 is arranged at the position of the liquid inlet pipe 5 near the refrigerator 9.

[0036] The portion of the liquid outlet pipe 6 located in the heat exchange box 7 is S-shaped, which increases the stroke of the liquid outlet pipe 6 in the heat exchange box 7, thereby increasing the area of ​​heat exchange. A first fan 14 is provided on the top wall of the heat exchange box 7 near the liquid outlet pipe 6, and a plurality of air inlet holes 18 are provided on the top wall of the heat exchange box 7 near the first fan 14. A plurality of air outlet holes 19 are provided on the lower portion of the side wall of the heat exchange box 7 near the liquid outlet pipe 6 to perform the first cooling of the refluxed coolant, so as to facilitate rapid cooling after entering the refrigerator 9.

[0037] A shielding sheet 13 is provided on the outer wall of the heat exchange box 7 near the upper part of each air outlet 19. The shielding sheet 13 is tilted downward to change the flow direction of the exhausted hot air so that it blows diagonally downward to prevent the hot air from blowing directly onto the experimenter.

[0038] A controller 17 is provided on the upper part of the heat exchange box 7, and four temperature sensors 16 are respectively provided on the upper and lower parts of the inner wall of the box body 1. The controller 17 is electrically connected to the water pump 8, the first fan 14, and the temperature sensor 16 respectively. The eight temperature sensors 16 are used to detect the temperature values ​​at the upper corner positions and the four lower positions in the box body 1, and feed the temperature back to the controller 17.

[0039] A plurality of protrusions 21 are provided at the bottom of the box body 1 for lifting the film transfer box and suspending it in the air to facilitate air circulation under the film transfer box.

[0040] A second fan 15 is arranged at the bottom of the box body 1, and the second fan 15 is arranged between multiple protrusions 21. A third fan 22 is arranged at the lower part of the cover body 2. The second fan 15 and the third fan 22 are electrically connected to the controller 17. Through the operation of the second fan 15 and the third fan 22, convection of air is generated at the upper and lower parts of the transfer box, thereby driving convection of air inside the box body 1 and accelerating the heat exchange rate of the heat exchange plate 4.

[0041] Working principle: During the experiment, the transfer box is placed on the multiple protrusions 21 inside the box body 1. The multiple protrusions 21 form a gap between the bottom of the transfer box and the bottom of the box body 1. Then the cover body 2 is covered on the box body 1 to keep it sealed. Then the coolant is added to the refrigerator 9 through the injection pipe 12. The refrigerator 9 is started by the controller 17. The refrigerator 9 cools the coolant through the multiple cooling plates 10 therein. When the refrigerator 9 is started, the stirrer 11 therein is automatically started to stir the coolant to make the temperature of the coolant balanced. Then The water pump 8 is started by the controller 17. The water pump 8 draws the coolant in the refrigerator 9 through the liquid inlet pipe 5 and sends it into the cooling pipe 3. The cooling pipe 3 and the inside of the box body 1 are quickly heat exchanged through the heat exchange plate 4, so that the temperature inside the box body 1 is reduced to a temperature range suitable for the mold transfer experiment. At the same time, the heat generated during the film transfer box experiment is taken away by the coolant, so that the temperature inside the box body 1 is maintained within a temperature range suitable for the mold transfer experiment. At the same time, 8 temperature sensors 16 detect the temperature at different positions inside the box body 1, and feed back the detected temperature value to the controller 17.

[0042] When the temperature value fed back by one or more of the temperature sensors 16 is greater than the temperature range suitable for the mold transfer experiment, it means that the heat generated by the film transfer box is too much, and the first fan 14, the second fan 15, and the third fan 22 are started by the controller 17. Through the operation of the second fan 15 and the third fan 22, the air in the upper and lower parts of the film transfer box is convected, so that the air in various positions inside the box body 1 is circulated. On the one hand, the temperature inside the box body 1 is balanced, and on the other hand, the heat exchange rate of the heat exchange plate 4 is accelerated, so that the temperature inside the box body 1 can be quickly taken away by the coolant. When the reflux coolant passes through the S-shaped part of the liquid outlet pipe 6, the first fan 14 is used to cool the reflux coolant for the first time, so that it is convenient for it to enter the refrigerator 9 and then be cooled quickly.

[0043] When the temperature values ​​fed back by the eight temperature sensors 16 are simultaneously lower than the temperature range suitable for the rotational mold experiment, the first fan 14 is shut down through the controller 17, so that the coolant is cooled only in the refrigerator 9, and the eight temperature sensors 16 are continued to detect the temperature at different positions inside the box body 1. If the temperature values ​​fed back by the eight temperature sensors 16 are still lower than the temperature range suitable for the rotational mold experiment, the second fan 15 and the third fan 22 are shut down through the controller 17, and the above operation is repeated in this way to keep the temperature inside the box body 1 always within the temperature range suitable for the rotational mold experiment.

[0044] The protein blotting transfer cooling device of the present invention has the following advantages:

[0045] First, by setting the cooling component and the heat exchange component, the temperature inside the box body generated by the film transfer box during the mold transfer process can be quickly heat exchanged through the cooling component, so as to quickly reduce the temperature inside the box body to a temperature range suitable for the mold transfer experiment. At the same time, the heat generated during the film transfer box experiment is taken away by the coolant, providing a suitable experimental temperature for the mold transfer experiment and improving the efficiency of the mold transfer. At the same time, the heat exchange component can quickly cool the cooling liquid with a high temperature, so that the cooling liquid can be recycled, saving the experimental cost;

[0046] Second, by setting up the second and third fans, the air inside the box can generate convection, which accelerates the heat exchange rate of the heat exchanger and quickly reduces the temperature inside the box, thus providing a guarantee for the mold rotation experiment.

[0047] Third, by cooperating with the first fan and the S-shaped liquid outlet pipe, the returning coolant is cooled for the first time, so that it is easy to cool down quickly after entering the refrigerator, thereby improving the cooling rate of the coolant;

[0048] Fourth, by coordinating the controller and the temperature sensor, the temperature inside the box is monitored in real time, so that the temperature inside the box can always be maintained in a temperature range suitable for the mold transfer experiment, further improving the efficiency of the mold transfer. At the same time, the box body and the cover are made of thermal insulation materials to avoid the external environment from affecting the temperature inside the box, ensuring that the temperature inside the box remains stable.

[0049] It is to be understood that the present invention is described by some embodiments, and it is known to those skilled in the art that various changes or equivalent substitutions may be made to these features and embodiments without departing from the spirit and scope of the present invention. In addition, under the teachings of the present invention, these features and embodiments may be modified to adapt to specific circumstances and materials without departing from the spirit and scope of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the scope of protection of the present invention.

Claims

1. A protein blotting transfer cooling device, characterized in that: include: The box body (1) has an open structure at the top and a hollow structure on the side wall to form a cooling chamber (20); A cover body (2) is arranged at the upper opening of the box body (1) and is buckled on the opening of the box body (1); A heat exchange box (7) is arranged on the side of the box body (1) and is connected to the box body (1) via a liquid inlet pipe (5) and a liquid outlet pipe (6); A cooling component is arranged inside the box body (1), and is respectively connected to one end of the liquid inlet pipe (5) and one end of the liquid outlet pipe (6), and is used to cool the transfer box placed inside the box body (1); A heat exchange component is arranged inside the heat exchange box (7), and is respectively connected to the other end of the liquid inlet pipe (5) and the other end of the liquid outlet pipe (6), and is used to provide cooling liquid to the liquid inlet pipe (5) and to cool the cooling liquid returning from the liquid outlet pipe (6); The cooling assembly comprises a cooling tube (3), wherein the cooling tube (3) is arranged inside the cooling cavity (20), and the cooling tube (3) is arranged in an S shape around the side wall of the box body (1), and a plurality of heat exchange fins (4) are evenly arranged between the S-shaped cooling tubes (3), and the plurality of heat exchange fins (4) are arranged around the side wall of the box body (1), and one end of each heat exchange fin (4) is fixedly connected to an inner wall on one side of the cooling cavity (20), and the other end of each heat exchange fin (4) passes through the inner wall on the other side of the cooling cavity (20) and is located inside the box body (1), the upper end of the cooling tube (3) is connected to one end of the liquid outlet pipe (6), and the lower end of the cooling tube (3) is connected to one end of the liquid inlet pipe (5); The heat exchange assembly comprises a refrigerator (9), which is arranged inside a heat exchange box (7). A plurality of refrigeration plates (10) and a stirrer (11) are arranged inside the refrigerator (9). The other end of the liquid inlet pipe (5) passes through the side wall of the heat exchange box (7) and is connected to the side of the refrigerator (9). The other end of the liquid outlet pipe (6) passes through another side wall of the heat exchange box (7) and is connected to another side of the refrigerator (9). The refrigerator (9) is respectively connected to the liquid outlet pipe (6) and the liquid inlet pipe (5). A liquid injection pipe (12) is vertically arranged on the upper part of the heat exchange box (7). The lower end of the liquid injection pipe (12) passes through the top wall of the heat exchange box (7) and is connected to the refrigerator (9). The liquid injection pipe (12) is connected to the refrigerator (9). A water pump (8) is arranged on the liquid inlet pipe (5) near the refrigerator (9).

2. A protein blot transfer cooling device according to claim 1, characterized in that: The portion of the liquid outlet pipe (6) located in the heat exchange box (7) is S-shaped, a first fan (14) is provided on the top wall of the heat exchange box (7) near the liquid outlet pipe (6), a plurality of air inlet holes (18) are provided on the top wall of the heat exchange box (7) near the first fan (14), and a plurality of air outlet holes (19) are provided on the lower portion of the side wall of the heat exchange box (7) near the liquid outlet pipe (6).

3. A protein blot transfer cooling device according to claim 2, characterized in that: A shielding sheet (13) is provided on the outer wall of the heat exchange box (7) near the upper part of each air outlet (19), and the shielding sheet (13) is inclined downward.

4. A protein blot transfer cooling device according to claim 1, characterized in that: A controller (17) is provided at the upper portion of the heat exchange box (7), and a plurality of temperature sensors (16) are provided at the upper portion and the lower portion of the inner wall of the box body (1), respectively. The controller (17) is electrically connected to the water pump (8), the first fan (14), and the temperature sensor (16), respectively.

5. A protein blot transfer cooling device according to claim 4, characterized in that: The inner bottom of the box body (1) is provided with a plurality of protrusions (21).

6. A protein blot transfer cooling device according to claim 5, characterized in that: A second fan (15) is arranged at the bottom of the box body (1), and the second fan (15) is arranged between a plurality of protrusions (21). A third fan (22) is arranged at the lower part of the cover body (2), and the second fan (15) and the third fan (22) are both electrically connected to a controller (17).

7. A protein blot transfer cooling device according to claim 1, characterized in that: The box body (1) and the cover body (2) are both made of heat-insulating materials.

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