A method for detecting biological toxins in feed

Abstract

The present invention relates to the technical field of toxin detection, and specifically to a detection method for detecting biological toxins in feed, comprising the following steps: step 1: sample selection, step 2: sample dissolution, step 3: sample liquid extraction, step 4: sample liquid detection. In the scheme of the present application, by adopting the detection method, the dissolution degree of the sample in the dissolving liquid is improved. When the detected sample contains biological toxins, the sampling head can reduce the situation where the biological substance is not dissolved or dissolved in a small amount in the dissolving liquid when extracting the sample liquid, thereby reducing or even avoiding the situation where the sampling head fails to detect the biological toxin after the sample containing the biological toxin is prepared, thereby improving the accuracy of the detection of whether the feed contains biological toxins.

Description

Technical Field

[0001] The present invention relates to the technical field of toxin detection, and in particular to a detection method for detecting biological toxins in feed. Background Art

[0002] There are two terms for biological toxins: biological toxins and natural toxins. Biological toxins are toxic substances produced by various organisms (animals, plants, and microorganisms), while natural toxins refer to toxic chemical substances that are of biological origin and cannot replicate themselves, including various chemical substances produced by animals, plants, and microorganisms that are toxic to other biological species.

[0003] However, various biological toxin detection devices on the market still have various problems. For example, when the biological toxin detection devices in the prior art are actually working, the reagent tubes need to be frequently disassembled and assembled, which has a great impact on the actual test results and the efficiency of the test. In particular, the most common detection method currently uses a spectrometer to detect samples. The operation process is to first dissolve the feed sample in a solution to obtain a sample solution and mix it, then sample the sample solution through a dropper, and then prepare the sample for filming, and then send the prepared film to the spectrometer for detection. This method is the current mainstream method for feed toxin detection.

[0004] However, during further research, the inventors found that this method still has some shortcomings. The reason is that when dissolving the feed sample, if the feed sample is not soluble enough or the solubility is poor in the solution, when the sample to be tested contains biological toxins, when the sample liquid is sampled through a dropper, the biological substance will not be dissolved or will be dissolved in a small amount in the dissolving liquid. As a result, when the subsequent spectrometer is used for detection, the biological toxins are not detected in the prepared sample, which is not conducive to the accuracy of the detection of biological toxins. As a result, even if the feed consumed by pigs contains toxic components, it is difficult to obtain real feedback in the test results.

[0005] Based on this, we propose a detection method that can more truly reflect the biological toxins in feed. Summary of the invention

[0006] The purpose of the present invention is to provide a detection method that can better reflect the biological toxins in feed in view of the above-mentioned shortcomings existing in the actual operation of the current detection of biological toxins in feed.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] A method for detecting biotoxins in feed, comprising the following steps:

[0009] Step 1: Sample selection: select part of the feed to be tested, and the selected part of the feed is the sample;

[0010] Step 2: dissolving the sample, using a detection device, and placing the sample into a reagent tube provided on the detection device, adding a dissolving solution into the reagent tube, dissolving the sample in the dissolving solution, and obtaining a sample solution, and performing negative pressure treatment on the sample solution;

[0011] Step 3: extracting the sample liquid, inserting a sampling head into the reagent tube, making the sampling head contact the sample liquid, and extracting the sample liquid;

[0012] Step 4: Detection of the sample liquid, placing the sampling head with the sample liquid extracted on the spectrum analyzer, and using the spectrum analyzer to detect the sample liquid on the sampling head.

[0013] As a preferred technical solution of the present application, the negative pressure treatment in step 2 includes: extending the sampling head into the reagent tube and discharging the air in the reagent tube during the extension process, and then moving the sampling head in the direction of extending out of the reagent tube, and the sampling head adheres to the inner wall of the reagent tube during the movement process, so that the sample liquid is in a negative pressure environment.

[0014] As a preferred technical solution of the present application, during the negative pressure treatment, when the sampling head is inserted into the reagent tube, the sampling head is separated from the sample liquid.

[0015] As a priority technical solution of the present application, a one-way valve is provided on the sampling head, and the sampling head can be attached to the inner wall of the reagent tube and can perform piston movement in the reagent tube. In the process of the sampling head extending into the reagent tube, the gas in the space surrounded by the reagent tube and the sampling head is discharged from the one-way valve. In the process of the sampling head that has been extended into the reagent tube being taken out of the reagent tube, the one-way valve blocks the gas.

[0016] As a preferred technical solution of the present application, the sampling head includes a balloon that can be inflated and deflated, and the balloon is transparent or the surface of the balloon is coated; when the sample liquid is extracted in step 3, the balloon contacts the sample liquid and makes the sample liquid adhere to the surface of the balloon; in step 4, the balloon is continuously inflated during the detection of the sample liquid.

[0017] As a preferred technical solution of this application, the detection device includes a base plate, a support column, a protective seat, a supporting mechanism and a detection mechanism;

[0018] The support columns are movably mounted on the upper end surface of the bottom plate, and there are a plurality of support columns, which are symmetrically arranged about the center of the bottom plate;

[0019] The protective seat is installed on the upper end surface of the support column; the supporting mechanism includes a temperature control plate, the reagent tube is installed inside the temperature control plate, the upper end of the reagent tube extends to the upper end of the temperature control plate, and the lower end of the temperature control plate is connected to a motor through a motor shaft;

[0020] The detection mechanism includes two support plates, the inner side walls of the support plates are installed with cylinders, the output end of the cylinder is installed with a lifting column, the lower end of the lifting column is detachably connected to a fixing frame, a connecting rod is installed inside the fixing frame, the circumferential surface of the connecting rod is sleeved with two connecting seats, the lower end surfaces of the two connecting seats are installed with positioning rods, and the sampling head is installed on the lower end surface of the positioning rod.

[0021] As a preferred technical solution of the present application, a circular groove is provided on the upper end surface of the bottom plate, and the lower end of the support column performs a circular motion inside the groove.

[0022] As the priority technical solution of the present application, an installation cavity is provided inside the protective seat, the temperature control board is installed in the installation cavity, and a countersunk hole is opened at the center of the lower end surface of the temperature control board, and a connecting block matching the size of the countersunk hole is installed at the upper end of the motor shaft, and the connecting block is buckled in the countersunk hole.

[0023] As a preferred technical solution of the present application, a protective pad is sleeved on the surface of the lower end of the reagent tube, and an installation groove matching the size of the protective pad is opened on the upper end surface of the temperature control plate, and the protective pad is installed inside the protective pad.

[0024] As a preferred technical solution of the present application, a clamping block is installed on the lower end surface of the lifting column, and a clamping slot matching the size of the clamping block is opened at the center of the upper end surface of the fixing frame, and the clamping block is clamped in the clamping slot.

[0025] As a preferred technical solution of the present application, a U-shaped groove is provided on the lower end surface of the fixing frame, and both ends of the connecting rod are connected to the inner side walls of the U-shaped groove.

[0026] As a preferred technical solution of the present application, a through hole matching the outer diameter of the connecting rod is opened inside the connecting seat, and the connecting rod passes through the connecting seat through the through hole.

[0027] As a preferred technical solution of the present application, a bracket is installed on the upper end surface of the base plate, and the two support plates are installed on the upper end surface of the bracket.

[0028] As a preferred technical solution of the present application, a rectangular through slot matching the size of the fixing frame is provided at the upper end of the bracket, and the fixing frame forms a sliding connection with the bracket through the rectangular through slot.

[0029] As a preferred technical solution of the present application, the outer diameter of the sampling head is smaller than the inner diameter of the reagent tube.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] In the scheme of the present application, in the process of detecting biological toxins in feed, sample selection is first performed, and part of the feed is selected from the feed to be detected, and the selected part of the feed is used as the sample required for detection; then the sample is dissolved. In this step, a detection device is used, and the sample is placed in a reagent tube set on the detection device. At the same time, a dissolving solution is added to the reagent tube, so that the sample is dissolved in the dissolving solution, and a sample liquid is obtained. By subjecting the sample liquid to negative pressure treatment, the sample in the sample liquid is more easily dissolved in the dissolving solution under negative pressure, thereby improving the solubility of the sample in the dissolving solution. Since the process from dissolving the sample in the dissolving solution to precipitating from the dissolving solution is not completed instantaneously, a sampling head is inserted into the reagent tube so that the sampling head contacts the sample liquid. When the sample liquid is contacted with the sample liquid, the sample in the sample liquid does not precipitate or only precipitates a small amount, so that the sample in the extracted sample liquid has a better solubility, thereby realizing the extraction of the sample liquid. Then, the sampling head with the extracted sample liquid is placed on the spectrum analyzer, and the spectrum analyzer is used to detect the sample liquid on the sampling head. By adopting this detection method, the solubility of the sample in the dissolving liquid is improved. When the detected sample contains biological toxins, the sampling head can reduce the situation that the biological substance is not dissolved or dissolved in a small amount in the dissolving liquid when extracting the sample liquid, thereby reducing or even avoiding the situation that the sampling head does not detect the biological toxin after the sample containing the biological toxin is prepared, thereby improving the accuracy of the detection of whether the feed contains biological toxins;

[0032] In step 2, after the sample liquid is dissolved in the dissolving liquid, the sampling head is inserted into the reagent tube so that the air in the reagent tube can be discharged during the insertion process, wherein the area of ​​the discharged air in the reagent tube is the space where the sample body is placed, and then the sampling head is moved in the direction of extending out of the reagent tube, and the sampling head is attached to the inner wall of the reagent tube during the movement, so as to avoid the generation of gas or a large amount of gas entering the space surrounded by the reagent tube and the sampling head during the movement of the sampling head in the direction of extending out of the reagent tube, so that the sample liquid is in a negative pressure environment, thereby improving the solubility of the sample in the dissolving liquid, thereby improving the accuracy of the detection of biological toxins in the sample liquid;

[0033] In the negative pressure treatment, when the sampling head is inserted into the reagent tube, the sampling head is separated from the sample liquid, so that when the negative pressure treatment of the sample liquid is not completed, the sampling head can be prevented from absorbing or absorbing or being stained with the sample liquid. By adopting the method of separating the sampling head from the sample liquid when the sampling head is inserted into the reagent tube during the negative pressure treatment, the degree of dissolution of the sample in the sample liquid extracted in step 3 is better, thereby further improving the accuracy of the detection of biological toxins in the sample liquid;

[0034] A one-way valve is arranged on the sampling head, and the sampling head can be attached to the inner wall of the reagent tube and can perform piston movement in the reagent tube. The one-way valve is arranged so that when negative pressure treatment is performed, when the sampling head is inserted into the reagent tube, the gas in the space surrounded by the reagent tube and the sampling head is discharged from the one-way valve. At the same time, when the sampling head that has been inserted into the reagent tube is taken out of the reagent tube, the one-way valve blocks the gas, so that the space surrounded by the reagent tube and the sampling head is placed to exchange gas with the external space. The external space refers to the space outside the space surrounded by the reagent tube and the sampling head. The arrangement of the one-way valve improves the convenience of negative pressure treatment.

[0035] The sampling head includes a balloon that can be inflated and deflated. The balloon is set to be transparent or a coating is set on the surface of the balloon. The coating is a reflective layer, so that when the sample liquid is extracted in step 3, the balloon is in a state of not inflated or inflated with a small amount of gas, and the balloon contacts the sample liquid, so that the sample liquid adheres to the surface of the balloon. At the same time, when the sample liquid is detected in step 4, the liquid film of the sample liquid attached to the balloon has a certain thickness. By continuously inflating the balloon while detecting the sample liquid on the balloon, the surface area of ​​the balloon gradually increases, and at the same time, the thickness of the liquid film of the sample liquid on the balloon gradually decreases, making the liquid film thinner, so that the internal toxins can be blocked by other components or particles in front as much as possible, thereby increasing the exposure rate of the toxin components and thereby increasing the possibility of detecting the toxins. Furthermore, this method also allows the toxins inside the liquid film to be well sealed in the liquid film before the balloon is deformed without contacting the air. In this way, problems such as denaturation or oxidation caused by contact between the toxins and the air are directly avoided, thereby greatly improving the accuracy of biological toxin detection in feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 A flow chart of an implementation method of a detection method for detecting biological toxins in feed according to the present application;

[0037] Figure 2 This is a schematic diagram of the structure of a detection device in a detection method for detecting biological toxins in feed according to the present application;

[0038] Figure 3This is a schematic diagram of the structure of a detection device in a detection method for detecting biological toxins in feed according to the present application;

[0039] Figure 4 This is a structural schematic diagram of the positional relationship between a support column and a protective seat on a detection device in a detection method for detecting biological toxins in feed in the present application;

[0040] Figure 5 This is a schematic diagram of the structure of a supporting mechanism in a detection device in a detection method for detecting biological toxins in feed according to the present application;

[0041] Figure 6 This is a schematic diagram of the structure of a detection mechanism in a detection device in a detection method for detecting biological toxins in feed according to the present application;

[0042] Markings in the figure: 1. Base plate; 2. Support column; 3. Protective seat; 4. Supporting mechanism; 401. Temperature control board; 402. Reagent tube; 403. Motor shaft; 404. Motor; 405. Protective pad; 5. Bracket; 6. Detection mechanism; 601. Support plate; 602. Cylinder; 603. Lifting column; 604. Fixed frame; 605. Connecting rod; 606. Connecting seat; 607. Positioning rod; 608. Sampling head. DETAILED DESCRIPTION

[0043] To make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be described clearly and completely in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.

[0044] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents some embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features and technical solutions in the embodiments may be combined with each other.

[0046] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0047] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the invention product is usually placed when in use, or the orientation or positional relationship commonly understood by those skilled in the art. Such terms are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0048] Example 1: See Figure 1-6 As shown,

[0049] The present embodiment provides a method for detecting biological toxins in feed, comprising the following steps:

[0050] Step 1: Sample selection: select part of the feed to be tested, and the selected part of the feed is the sample;

[0051] Step 2: dissolving the sample, using a detection device, and placing the sample into a reagent tube 403 provided on the detection device, adding a dissolving solution into the reagent tube 403, dissolving the sample in the dissolving solution, and obtaining a sample solution, and performing negative pressure treatment on the sample solution;

[0052] Step 3: extracting the sample liquid, inserting the sampling head 608 into the reagent tube 403, making the sampling head 608 contact the sample liquid, and extracting the sample liquid;

[0053] Step 4: Detection of the sample liquid. Place the sampling head 608 containing the sample liquid on a spectrum analyzer, and use the spectrum analyzer to detect the sample liquid on the sampling head 608 .

[0054] In the process of detecting biological toxins in feed, sample selection is first performed, and part of the feed is selected from the feed to be detected, and the selected part of the feed is used as the sample required for detection; then the sample is dissolved. In this step, a detection device is used, and the sample is placed in a reagent tube 403 set on the detection device. At the same time, a dissolving liquid is added to the reagent tube 403, so that the sample is dissolved in the dissolving liquid, and a sample liquid is obtained. By subjecting the sample liquid to negative pressure treatment, the sample in the sample liquid is more easily dissolved in the dissolving liquid under negative pressure, thereby improving the solubility of the sample in the dissolving liquid. Since the process from dissolving the sample in the dissolving liquid to precipitating from the dissolving liquid is not completed instantaneously, the sampling head 608 is inserted into the reagent tube 403 so that the sampling head 608 contacts the sample Liquid, when it contacts the sample liquid, the sample in the sample liquid does not precipitate or only precipitates a small amount, so that the sample in the extracted sample liquid has a better solubility, thereby realizing the extraction of the sample liquid, and then the sampling head 608 with the extracted sample liquid is placed on the spectrometer, and the spectrometer is used to detect the sample liquid on the sampling head 608. By adopting this detection method, the solubility of the sample in the dissolving liquid is improved. When the detected sample contains biological toxins, the sampling head 608 can reduce the situation where the biological substance is not dissolved or dissolved in a small amount in the dissolving liquid when extracting the sample liquid, thereby reducing or even avoiding the situation where the sampling head 608 does not detect the biological toxins after the sample liquid containing the biological toxins is prepared, thereby improving the accuracy of the detection of whether the feed contains biological toxins.

[0055] As a preferred embodiment, on the basis of the above method, further, the negative pressure treatment in step 2 includes: extending the sampling head 608 into the reagent tube 403, and discharging the air in the reagent tube 403 during the extension process, and then moving the sampling head 608 in the direction of extending out of the reagent tube 403, and the sampling head 608 adheres to the inner wall of the reagent tube 403 during the movement process, so that the sample liquid is in a negative pressure environment.

[0056] In step 2, after the sample liquid is dissolved in the dissolving liquid, the sampling head 608 is inserted into the reagent tube 403 so that the air in the reagent tube 403 can be discharged during the insertion process, wherein the area of ​​the air discharged in the reagent tube 403 is the space where the sample body is placed, and then the sampling head 608 is moved in the direction of extending out of the reagent tube 403, and the sampling head 608 adheres to the inner wall of the reagent tube 403 during the movement, so as to avoid the generation of gas or a large amount of gas entering the space surrounded by the reagent tube 403 and the sampling head 608 when the sampling head 608 moves in the direction of extending out of the reagent tube 403, so that the sample liquid is in a negative pressure environment, thereby improving the solubility of the sample in the dissolving liquid, thereby improving the accuracy of biological toxin detection in the sample liquid.

[0057] As a preferred embodiment, based on the above-mentioned manner, further, during the negative pressure treatment, when the sampling head 608 is inserted into the reagent tube 403, the sampling head 608 is separated from the sample liquid.

[0058] During the negative pressure treatment, when the sampling head 608 is inserted into the reagent tube 403, the sampling head 608 is separated from the sample liquid, so that when the negative pressure treatment of the sample liquid is not completed, the sampling head 608 can be prevented from absorbing or absorbing or being stained with the sample liquid. By taking the approach of separating the sampling head 608 from the sample liquid when the sampling head 608 is inserted into the reagent tube 403 during the negative pressure treatment, the sample in the sample liquid extracted in step 3 is better in solubility, thereby further improving the accuracy of biological toxin detection in the sample liquid.

[0059] As a preferred embodiment, on the basis of the above-mentioned manner, further, a one-way valve is arranged on the sampling head 608, and the sampling head 608 can be attached to the inner wall of the reagent tube 403 and can perform piston movement in the reagent tube 403. When the sampling head 608 is extended into the reagent tube 403, the gas in the space surrounded by the reagent tube 403 and the sampling head 608 is discharged from the one-way valve. When the sampling head 608 that has been extended into the reagent tube 403 is taken out from the reagent tube 403, the one-way valve blocks the gas.

[0060] A one-way valve is arranged on the sampling head 608, and the sampling head 608 can be attached to the inner wall of the reagent tube 403 and can perform piston movement in the reagent tube 403. The setting of the one-way valve allows the gas in the space surrounded by the reagent tube 403 and the sampling head 608 to be discharged from the one-way valve during negative pressure treatment while the sampling head 608 is inserted into the reagent tube 403. At the same time, during the process of the sampling head 608 that has been inserted into the reagent tube 403 being taken out of the reagent tube 403, the one-way valve blocks the gas, thereby allowing the space surrounded by the reagent tube 403 and the sampling head 608 to exchange gas with the external space. The external space refers to the space outside the space surrounded by the reagent tube 403 and the sampling head 608. The setting of the one-way valve improves the convenience of negative pressure treatment.

[0061] As a preferred embodiment, on the basis of the above-mentioned method, further, the sampling head 608 includes a balloon that can be inflated and deflated, and the balloon is transparent or the surface of the balloon is coated; when the sample liquid is extracted in step 3, the balloon contacts the sample liquid and makes the sample liquid adhere to the surface of the balloon; in step 4, the balloon is continuously inflated during the detection of the sample liquid.

[0062] The sampling head 608 includes a balloon that can be inflated and deflated. The balloon is set to be transparent or a coating is set on the surface of the balloon. The coating is a reflective layer. When the sample liquid is extracted in step 3, the balloon is in a state of not inflated or inflated with a small amount of gas, and the balloon contacts the sample liquid, so that the sample liquid adheres to the surface of the balloon. At the same time, when the sample liquid is detected in step 4, the liquid film of the sample liquid attached to the balloon has a certain thickness. By continuously inflating the balloon while detecting the sample liquid on the balloon, the surface area of ​​the balloon gradually increases, and at the same time, the thickness of the liquid film of the sample liquid on the balloon gradually decreases, making the liquid film thinner, so that the internal toxins can be blocked by other components or particles in front as much as possible, thereby increasing the exposure rate of the toxin components and thereby increasing the possibility of detecting the toxins. Furthermore, this method also allows the toxins inside the liquid film to be well sealed in the liquid film before the balloon is deformed without contacting the air. In this way, problems such as denaturation or oxidation caused by contact between the toxins and the air are directly avoided, thereby greatly improving the accuracy of biological toxin detection in feed.

[0063] Example 2: See Figure 2-6 As shown,

[0064] On the basis of the technical solution of the first embodiment, further, the detection device includes a base plate 1, a support column 2, a protection seat 3, a supporting mechanism 4 and a detection mechanism 6;

[0065] The support column 2 is movably mounted on the upper end surface of the bottom plate 1. There are a plurality of support columns 2, and the support columns 2 are symmetrically arranged about the center of the bottom plate 1.

[0066] The protective seat 3 is installed on the upper end surface of the support column 2;

[0067] The supporting mechanism 4 includes a temperature control plate 401, and a reagent tube 402 is installed inside the temperature control plate 401. The upper end of the reagent tube 402 extends to the upper end of the temperature control plate 401. The lower end of the temperature control plate 401 is connected to a motor 404 through a motor shaft 403. After the motor 404 is started, the motor shaft 403 can be driven to make a circular motion, thereby driving the temperature control plate 401 to make a circular motion, and then the reagent tube 402 inside the temperature control plate 401 can be made to make a circular motion. The cooperation with the detection mechanism 6 can achieve the purpose of detection, without the need to frequently disassemble the reagent tube 402, with high work efficiency and strong practicality.

[0068] The detection mechanism 6 includes two support plates 601, and a cylinder 602 is installed on the inner wall of the support plate 601. A lifting column 603 is installed on the output end of the cylinder 602. The lower end of the lifting column 603 is detachably connected to a fixing frame 604. A connecting rod 605 is installed inside the fixing frame 604. Two connecting seats 606 are sleeved on the circumferential surface of the connecting rod 605. Positioning rods 607 are installed on the lower end surfaces of the two connecting seats 606. The sampling head 608 is installed on the lower end surface of the positioning rod 607; after starting the cylinder 602, the height adjustment of the lifting column 603 and the components at its lower end can be achieved, so that the height of the positioning rod 607 and the sampling head 608 at its lower end can be adjusted, that is, the depth of the sampling head 608 inside the reagent tube 402 can be adjusted, so that the detection needs of samples of different capacities can be met. It has strong practicality, a wide range of applications, is easy to promote and utilize, and at the same time improves the flexibility of the movement of the sampling head 608.

[0069] As a preferred embodiment, on the basis of the above-mentioned method, further, a circular groove is opened on the upper end surface of the base plate 1, and the lower end of the support column 2 makes a circular motion inside the groove; when the motor 404 is working, it will drive the temperature control plate 401 and the external protective seat 3 to make a circular motion, thereby driving the support column 2 to make a circular motion around the center of the motor 404. By setting the circular groove, a path and space can be provided for the circular motion of the support column 2, and it also has the function of limiting, which is highly practical, thereby ensuring the actual use effect.

[0070] As a preferred embodiment, on the basis of the above-mentioned method, further, an installation cavity is provided inside the protective seat 3, and the temperature control board 401 is installed in the installation cavity. By setting the installation cavity, the installation stability of the temperature control board 401 can be guaranteed, and the temperature control board 401 can be disassembled; and a countersunk hole is opened at the center of the lower end surface of the temperature control board 401, and a connecting block matching the size of the countersunk hole is installed on the upper end of the motor shaft 403, and the connecting block is buckled in the countersunk hole. Through the cooperation of the countersunk hole and the connecting block, the connection stability of the motor shaft 403 and the temperature control board 401 can be guaranteed, and the temperature control board 401 and the motor shaft 403 can be disassembled, which is convenient for operation.

[0071] As a preferred embodiment, on the basis of the above-mentioned method, further, a protective pad 405 is sleeved on the surface of the lower end of the reagent tube 402, and a mounting groove matching the size of the protective pad 405 is opened on the upper end surface of the temperature control plate 401, and the protective pad 405 is installed inside the protective pad 405; by setting the mounting groove, the installation stability of the protective pad 405 can be ensured, and under the action of the protective pad 405, the installation stability of the reagent tube 402 can be ensured, and the collision and damage of the reagent tube 402 and the temperature control plate 401 during rotation can be effectively avoided, and by replacing protective pads 405 of different sizes, the use requirements of reagent tubes 402 of different diameters can be met.

[0072] As a preferred embodiment, on the basis of the above method, further, a card block is installed on the lower end surface of the lifting column 603, and a card slot matching the size of the card block is opened at the center of the upper end surface of the fixed frame 604, and the card block is connected in the card slot; through the cooperation between the card block and the card slot, the connection stability between the lifting column 603 and the fixed frame 604 can be ensured, and it is easy to disassemble and operate, with good use effect and strong practicality.

[0073] As a preferred embodiment, on the basis of the above method, further, a U-shaped groove is provided on the lower end surface of the fixing frame 604, and both ends of the connecting rod 605 are connected to the inner wall of the U-shaped groove; by providing the U-shaped groove, the installation stability of the connecting rod 605 can be ensured, thereby achieving the protection purpose of the connecting seat 606, which is highly practical and easy to operate.

[0074] As a preferred embodiment, on the basis of the above method, further, a through hole adapted to the outer diameter of the connecting rod 605 is opened inside the connecting seat 606, and the connecting rod 605 passes through the connecting seat 606 through the through hole; this arrangement can ensure the working stability of the connecting rod 605 and the connecting seat 606.

[0075] As a preferred embodiment, on the basis of the above method, further, a bracket 5 is installed on the upper end surface of the base plate 1, and two support plates 601 are installed on the upper end surface of the bracket 5; by setting the bracket 5, the supporting purpose of the support plate 601 can be guaranteed, thereby ensuring the use effect of the detection mechanism 6.

[0076] As a preferred embodiment, on the basis of the above-mentioned method, further, a rectangular through groove adapted to the size of the fixing frame 604 is opened at the upper end of the bracket 5, and the fixing frame 604 forms a sliding connection with the bracket 5 through the rectangular through groove; by setting the rectangular through groove, space can be provided for the movement of the fixing frame 604, and by having a limiting function, the fixing frame 604 can be prevented from falling off from the inside of the rectangular through groove.

[0077] As a preferred embodiment, on the basis of the above-mentioned method, further, the outer diameter of the sampling head 608 is smaller than the inner diameter of the reagent tube 402; this arrangement can ensure the use effect of the sampling head 608 and has strong practicality.

[0078] The above embodiments are only used to illustrate the present invention and are not intended to limit the technical solutions described in the present invention. Although the present invention has been described in detail with reference to the above embodiments, the present invention is not limited to the above specific implementation methods. Therefore, any modification or equivalent replacement of the present invention; and all technical solutions and improvements thereof that do not depart from the spirit and scope of the invention are included in the scope of the claims of the present invention.

Claims

1. A method for detecting biological toxins in feed, characterized in that , including the following steps: Step 1: Sample selection: select part of the feed to be tested, and the selected part of the feed is the sample; Step 2: dissolving the sample, using a detection device, and placing the sample into a reagent tube provided on the detection device, adding a dissolving solution into the reagent tube, dissolving the sample in the dissolving solution, and obtaining a sample solution, and performing negative pressure treatment on the sample solution; Step 3: extracting the sample liquid, inserting a sampling head into the reagent tube, making the sampling head contact the sample liquid, and extracting the sample liquid; Step 4: testing the sample liquid, placing the sampling head with the sample liquid extracted on the spectrum analyzer, and using the spectrum analyzer to test the sample liquid on the sampling head; The negative pressure treatment in step 2 includes: inserting the sampling head into the reagent tube, discharging the air in the reagent tube during the inserting process, and then moving the sampling head in a direction of extending out of the reagent tube, and the sampling head adheres to the inner wall of the reagent tube during the movement process, so that the sample liquid is in a negative pressure environment; During the negative pressure treatment, when the sampling head is inserted into the reagent tube, the sampling head is separated from the sample liquid; The sampling head is provided with a one-way valve, and the sampling head can be attached to the inner wall of the reagent tube and can perform piston movement in the reagent tube. When the sampling head is inserted into the reagent tube, the gas in the space surrounded by the reagent tube and the sampling head is discharged from the one-way valve. When the sampling head that has been inserted into the reagent tube is taken out of the reagent tube, the one-way valve blocks the gas. The sampling head includes a balloon that can be inflated and deflated, and the balloon is transparent or has a coating on its surface; when the sample liquid is extracted in step 3, the balloon contacts the sample liquid and makes the sample liquid adhere to the surface of the balloon; in step 4, the balloon is continuously inflated during the detection of the sample liquid; The detection device comprises a base plate, a support column, a protective seat, a supporting mechanism and a detection mechanism; The support columns are movably mounted on the upper end surface of the bottom plate, and there are a plurality of support columns, which are symmetrically arranged about the center of the bottom plate; The protective seat is installed on the upper end surface of the support column; the supporting mechanism includes a temperature control plate, the reagent tube is installed inside the temperature control plate, the upper end of the reagent tube extends to the upper end of the temperature control plate, and the lower end of the temperature control plate is connected to a motor through a motor shaft; The detection mechanism includes two support plates, the inner side walls of the support plates are installed with cylinders, the output end of the cylinder is installed with a lifting column, the lower end of the lifting column is detachably connected to a fixing frame, a connecting rod is installed inside the fixing frame, the circumferential surface of the connecting rod is sleeved with two connecting seats, the lower end surfaces of the two connecting seats are installed with positioning rods, and the sampling head is installed on the lower end surface of the positioning rod.

2. The detection method according to claim 1, characterized in that: The upper end surface of the bottom plate is provided with a circular sinking groove, and the lower end of the support column performs a circular motion inside the sinking groove.

3. The detection method according to claim 2, characterized in that: An installation cavity is provided inside the protective seat, the temperature control board is installed in the installation cavity, and a countersunk hole is opened at the center of the lower end surface of the temperature control board. A connecting block matching the size of the countersunk hole is installed on the upper end of the motor shaft, and the connecting block is buckled in the countersunk hole.

4. The detection method according to claim 3, characterized in that: A protective pad is sleeved on the surface of the lower end of the reagent tube, and a mounting groove matching the size of the protective pad is opened on the upper end surface of the temperature control plate, and the protective pad is installed inside the protective pad.

5. The detection method according to claim 4, characterized in that: A clamping block is installed on the lower end surface of the lifting column, and a clamping slot matching the size of the clamping block is opened at the center of the upper end surface of the fixing frame, and the clamping block is clamped in the clamping slot.

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