Ketoreductase polypeptides and polynucleotides

Abstract

The present invention provides engineered ketoreductases having improved properties compared to naturally occurring wild-type ketoreductases, polynucleotides encoding the engineered ketoreductases, host cells capable of expressing the engineered ketoreductases, and methods for synthesizing chiral alcohols using the engineered ketoreductases. The present invention also provides methods of using the engineered enzymes.

Description

[0001] This application claims the benefit of U.S. Provisional Patent Application Serial No. 62 / 906,268, filed September 26, 2019, which is hereby incorporated by reference in its entirety for all purposes.

[0002] Reference to a Sequence Listing, Table, or Computer Program

[0003] The Sequence Listing, submitted herewith in computer-readable form (CRF) via EFS-Web as file CX8-195WO2_ST25.txt, in accordance with 37 C.F.R. § 1.821, is hereby incorporated by reference. The electronic copy of the Sequence Listing was created on September 23, 2020, and is 664 kilobytes in size. Field of the Invention

[0004] The present invention provides engineered ketoreductases having improved properties compared to naturally occurring wild-type ketoreductases, polynucleotides encoding the engineered ketoreductases, host cells capable of expressing the engineered ketoreductases, and methods of using the engineered ketoreductases.

[0005] Background

[0006] Enzymes belonging to the ketoreductase (KRED) or carbonyl reductase class (EC 1.1.1.184) can be used to synthesize optically active alcohols from the corresponding prochiral ketone substrates and by the stereoselective reduction of the corresponding racemic aldehyde substrates. KREDs typically convert ketone substrates and aldehyde substrates into the corresponding alcohol products, but can also catalyze the reverse reaction, oxidizing alcohol substrates to the corresponding ketone / aldehyde products. Enzymes such as KREDs require cofactors for the reduction of ketones and aldehydes and for the oxidation of alcohols, most commonly reduced nicotinamide adenine dinucleotide (NADH) or reduced nicotinamide adenine dinucleotide phosphate (NADPH), and nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+) for the oxidation reaction. NADH and NADPH act as electron donors, while NAD+ and NADP+ act as electron acceptors. It is often observed that ketoreductases and alcohol dehydrogenases accept phosphorylated or non-phosphorylated cofactors (in their oxidized and reduced states), but most commonly not both.

[0007] To bypass many chemical synthesis procedures for the production of key compounds, ketoreductases are increasingly being used to enzymatically convert different ketone substrates and aldehyde substrates into chiral alcohol products. These applications can utilize whole cells expressing ketoreductases to biocatalyze the reduction of ketones and aldehydes or the biocatalysis of the oxidation of alcohols, or purified enzymes in those cases where the presence of multiple ketoreductases in whole cells would adversely affect the stereopurity and yield of the desired product.

[0008] "Bitterness" is an important tasting attribute of beer and typically comes from the addition of hops (the flowers of the plant Humulus lupulus L.). Iso-α-acids are formed during the brewing process by the isomerization of humulone, a compound naturally present in the lupulin glands of the hop plant. Specifically, six major iso-α-acids are responsible for bitterness: cis-isohumulone, trans-isohumulone, cis-isocohumulone, trans-isocohumulone, cis-isoadhumulone, and trans-isoadhumulone.

[0009] However, iso-α-acids are not light-stable, and the photo-induced formation of 3-methyl-2-buten-1-thiol (3-MBT) gives beer a distinct lightstruck or skunky flavor and aroma. This requires packaging beer in brown bottles or cans. Another solution is to produce a completely light-stable beer by reducing the carbonyl group of iso-α-acids to generate the corresponding dihydro-(ρ)-iso-α-acids. These reduced dihydro-(ρ)-iso-α-acids are stable and can be packaged in clear or green bottles.

[0010] However, currently, only toxic, dangerous, and non-food grade chemicals (such as sodium borohydride) can be used to convert iso-α-acids to dihydro-(ρ)-iso-α-acids. Therefore, the safe and food-grade conversion of iso-α-acids to dihydro-(ρ)-iso-α-acids has significant commercial value. Summary of the Invention

[0012] The present invention provides engineered ketoreductases having improved properties compared to naturally occurring wild-type ketoreductases, polynucleotides encoding the engineered ketoreductases, host cells capable of expressing the engineered ketoreductases, and methods of using the engineered ketoreductases.

[0013] The present invention provides engineered ketoreductases ("KREDs") having improved enzymatic activity in converting iso-α-acids to the corresponding dihydro-(ρ)-iso-α-acids compared to a naturally occurring wild-type ketoreductase from Lactobacillus kefir (SEQ ID NO:2) or when compared to other engineered ketoreductases (including engineered ketoreductase polypeptides of SEQ ID NO:4, 6, 80, 104, 172, 186, 194, 252, 270, 272, 286, 328, and / or 330).

[0014] In some additional embodiments, the engineered enzyme has one or more improved properties in addition to improved enzyme activity. Improvements in enzyme properties include, but are not limited to, improved activity across a range of substrates, improved activity at high substrate concentrations, and improved activity at low cofactor concentrations.

[0015] The present invention provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity with SEQ ID NO: 4, 6, 80, 104, 172, 186, 194, 252, 270, 272, 286, 328, and / or 330.

[0016] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity with SEQ ID NO: 4 and have at least one substitution or set of substitutions at one or more positions selected from the following: 12, 21, 87, 93, 97, 110, 145, 148, 152, 153, 194, 196, 197, 200, 206, 212, and 226, where the positions are numbered with reference to SEQ ID NO: 4. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: 12I, 21R, 87L, 93D, 93M, 93T, 93V, 97G, 110I, 145C, 145G, 145M, 145S, 148I, 152G, 152S, 153C, 153R, 153V, 194H, 194N, 194R, 196H, 196K, 196R, 197G, 197R, 200L, 200Q, 200R, 206V, 212S, and 226L, where the positions are numbered with reference to SEQ ID NO: 4. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: V12I, L21R, V87L, I93D, I93M, I93T, I93V, K97G, L110I, L145C, L145G, L145M, L145S, V148I, T152G, T152S, L153C, L153R, L153V, P194H, P194N, P194R, L196H, L196K, L196R, D197G, D197R, E200L, E200Q, E200R, M206V, T212S, and I226L, where the positions are numbered with reference to SEQ ID NO: 4.

[0017] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity with SEQ ID NO:6 and have at least one substitution or set of substitutions at one or more positions selected from the following: 12 / 110 / 145 / 152, 12 / 145, 87 / 110 / 145, 87 / 110 / 145 / 194, 87 / 145 / 194, 110, 110 / 145 / 152 / 197, 110 / 145 / 194, 145, 145 / 152, 145 / 197 / 226 and 152, where the positions are numbered with reference to SEQ ID NO:6. In some embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: 12I / 110I / 145M / 152G, 12I / 145M, 87L / 110I / 145M, 87L / 110I / 145M / 194H, 87L / 110I / 145M / 194N, 87L / 145M / 194H, 110I, 110I / 145M / 152G / 197G, 110I / 145M / 194H, 145M, 145M / 152G, 145M / 197G / 226L and 152S, where the positions are numbered with reference to SEQ ID NO:6. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: V12I / L110I / L145M / T152G, V12I / L145M, V87L / L110I / L145M, V87L / L110I / L145M / P194H, V87L / L110I / L145M / P194N, V87L / L145M / P194H, L110I, L110I / L145M / T152G / D197G, L110I / L145M / P194H, L145M, L145M / T152G, L145M / D197G / I226L and T152S, where the positions are numbered with reference to SEQ ID NO:6.

[0018] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO:80 and have at least one substitution or set of substitutions at one or more positions selected from the following: 17, 21, 46, 56, 72, 79, 95, 101, 110, 152, 162, 190, 198, 210, 211 and 227, where the positions are numbered with reference to SEQ ID NO:80. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: 17Q, 17S, 21A, 46V, 56C, 72A, 79L, 95I, 101C, 101L, 101T, 110V, 152K, 152L, 162G, 190A, 198A, 198Q, 210F, 210W, 211R and 227V, where the positions are numbered with reference to SEQ ID NO:80. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: L17Q, L17S, L21A, K46V, V56C, K72A, E79L, V95I, D101C, D101L, D101T, I110V, T152K, T152L, A162G, P190A, D198A, D198Q, T210F, T210W, L211R and C227V, where the positions are numbered with reference to SEQ ID NO:80.

[0019] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO:80 and have at least one substitution or set of substitutions at one or more positions selected from the following: 17, 79, 157, 159, 190 / 191 / 194, 190 / 194, 191 / 194, 194, 198, and 211, where the positions are numbered with reference to SEQ ID NO:80. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: 17M, 17Q, 17S, 79L, 157C, 159T, 190A / 191T / 194E, 190A / 194E, 191T / 194E, 194E, 198A, 198Q, and 211R, where the positions are numbered with reference to SEQ ID NO:80. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: L17M, L17Q, L17S, E79L, N157C, S159T, P190A / I191T / P194E, P190A / P194E, I191T / P194E, P194E, D198A, D198Q, and L211R, where the positions are numbered with reference to SEQ ID NO:80.

[0020] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO: 104 and have at least one substitution or set of substitutions at one or more positions selected from: 17 / 46 / 190, 17 / 46 / 198 / 211, 17 / 96 / 194 / 198, 17 / 190 / 198, 46 / 190 / 194 / 198, and 46 / 194 / 198, where the positions are numbered with reference to SEQ ID NO: 104. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: 17M / 46V / 190A, 17M / 46V / 198A / 211R, 17M / 96V / 194E / 198Q, 17M / 190A / 198A, 17M / 190A / 198Q, 46V / 190A / 194E / 198Q, and 46V / 194E / 198Q, where the positions are numbered with reference to SEQ ID NO: 104. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: Q17M / K46V / P190A, Q17M / K46V / D198A / L211R, Q17M / I96V / P194E / D198Q, Q17M / P190A / D198A, Q17M / P190A / D198Q, K46V / P190A / P194E / D198Q, and K46V / P194E / D198Q, where the positions are numbered with reference to SEQ ID NO: 104.

[0021] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO: 172 and have at least one substitution or set of substitutions at one or more positions selected from the following positions: 45, 101, 179, 194, 204, 226, and 231, where the positions are numbered with reference to SEQ ID NO: 172. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: 45L, 101R, 101Y, 179M, 194E, 204Q, 226V, and 231G, where the positions are numbered with reference to SEQ ID NO: 172. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: E45L, D101R, D101Y, Y179M, P194E, E204Q, I226V, and A231G, where the positions are numbered with reference to SEQ ID NO: 172.

[0022] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO: 186 and have at least one substitution or set of substitutions at one or more positions selected from the following positions: 95 / 96 / 97 / 150 / 153 / 205, 95 / 96 / 150 / 153 / 205 / 206 / 211 / 249, 95 / 97 / 143 / 145 / 150 / 153 / 202 / 205, 95 / 97 / 143 / 145 / 150 / 153 / 249, 95 / 97 / 150 / 153, 95 / 97 / 150 / 153 / 202 / 205 / 206, 95 / 150 / 153 / 205 / 206 / 211, 95 / 150 / 153 / 205 / 211, 95 / 150 / 153 / 206 / 249, 96 / 150 / 153, 96 / 150 / 153 / 206, 97 / 150 / 153, 97 / 150 / 153 / 205, 97 / 150 / 153 / 205 / 211, 97 / 150 / 153 / 206, 143 / 144 / 145 / 150 / 153 / 202 / 205 / 249, 143 / 145 / 150 / 153, 144 / 145 / 150 / 153 / 205 / 206, 144 / 150 / 153, 144 / 150 / 153 / 202 / 205 / 206, 145 / 150 / 153 / 206 / 249, 145 / 153 / 211, 150 / 153 / 202 / 206 / 249, 150 / 153 / 205 / 211, 150 / 153 / 206 / 211, 150 / 153 / 211 and 150 / 153 / 249, where the positions are numbered with reference to SEQ ID NO: 186.In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: 95A / 96A / 97A / 150A / 153A / 205A, 95A / 96A / 150A / 153A / 205A / 206A / 211A / 249A, 95A / 97A / 143A / 145A / 150A / 153A / 202A / 205A, 95A / 97A / 143A / 145A / 150A / 153A / 249A, 95A / 97A / 150A / 153A, 95A / 97A / 150A / 153A / 202A / 205A / 206A, 95A / 150A / 153A / 205A / 206A / 211A, 95A / 150A / 153A / 205A / 211A, 95A / 150A / 153A / 206A / 249A, 96A / 150A / 153A, 96A / 150A / 153A / 206A, 97A / 150A / 153A, 97A / 150A / 153A / 205A, 97A / 150A / 153A / 205A / 211A, 97A / 150A / 153A / 206A, 143A / 144A / 145A / 150A / 153A / 202A / 205A / 249A, 143A / 145A / 150A / 153A, 144A / 145A / 150A / 153A / 205A / 206A, 144A / 150A / 153A, 144A / 150A / 153A / 202A / 205A / 206A, 145A / 150A / 153A / 206A / 249A, 145A / 153A / 211A, 150A / 153A / 202A / 206A / 249A, 150A / 153A / 205A / 211A, 150A / 153A / 206A / 211A, 150A / 153A / 211A and 150A / 153A / 249A, wherein the positions are numbered with reference to SEQ ID NO:186.In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: V95A / I96A / K97A / D150A / L153A / M205A, V95A / I96A / D150A / L153A / M205A / M206A / L211A / W249A, V95A / K97A / S143A / M145A / D150A / L153A / W202A / M205A, V95A / K97A / S143A / M145A / D150A / L153A / W249A, V95A / K97A / D150A / L153A, V95A / K97A / D150A / L153A / W202A / M205A / M206A, V95A / D150A / L153A / M205A / M206A / L211A, V95A / D150A / L153A / M205A / L211A, V95A / D150A / L153A / M206A / W249A, I96A / D150A / L153A, I96A / D150A / L153A / M206A, K97A / D150A / L153A, K97A / D150A / L153A / M205A, K97A / D150A / L153A / M205A / L211A, K97A / D150A / L153A / M206A, S143A / I144A / M145A / D150A / L153A / W202A / M205A / W249A, S143A / M145A / D150A / L153A, I144A / M145A / D150A / L153A / M205A / M206A, I144A / D150A / L153A, I144A / D150A / L153A / W202A / M205A / M206A, M145A / D150A / L153A / M206A / W249A, M145A / L153A / L211A, D150A / L153A / W202A / M206A / W249A, D150A / L153A / M205A / L211A, D150A / L153A / M206A / L211A, D150A / L153A / L211A, and D150A / L153A / W249A, wherein the positions are numbered with reference to SEQ ID NO:186.

[0023] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity with SEQ ID NO: 186 and have at least one substitution or set of substitutions at one or more positions selected from: 7 / 147, 103 / 147, 110, 110 / 179 / 194, 147, and 249, where the positions are numbered with reference to SEQ ID NO: 186. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: 7Q / 147I, 103R / 147I, 110V, 110V / 179M / 194E, 147I, and 249Y, where the positions are numbered with reference to SEQ ID NO: 186. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: H7Q / L147I, T103R / L147I, I110V, I110V / Y179M / P194E, L147I, and W249Y, where the positions are numbered with reference to SEQ ID NO: 186.

[0024] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO: 194 and have at least one substitution or set of substitutions at one or more positions selected from: 7 / 12 / 54 / 110 / 150 / 153 / 194 / 205 / 211 / 249, 12 / 54 / 72 / 110 / 150 / 152 / 153 / 194 / 205 / 211 / 249, 12 / 72 / 101 / 103 / 110 / 152 / 249, 12 / 72 / 110 / 147 / 152 / 204, 45 / 54 / 72 / 110 / 152 / 194 / 204, 72 / 110 / 147 / 150 / 152 / 153 / 194 / 205 / 211 / 249 and 110 / 150 / 153 / 179 / 194 / 205 / 211 / 249, wherein the positions are numbered with reference to SEQ ID NO: 194. In some additional embodiments, the engineered ketoreductase variants comprise at least one substitution or set of substitutions selected from: 7Q / 12I / 54S / 110V / 150D / 153L / 194E / 205M / 211L / 249Y, 12I / 54S / 72T / 110V / 150D / 152M / 153L / 194E / 205M / 211L / 249Y, 12I / 72S / 101Y / 103Q / 110V / 152M / 249Y, 12I / 72S / 110V / 147I / 152M / 204Q, 45L / 54S / 72S / 110V / 152M / 194E / 204Q, 72S / 110V / 147M / 150D / 152M / 153L / 194E / 205M / 211L / 249Y and 110V / 150D / 153L / 179M / 194E / 205M / 211L / 249Y, wherein the positions are numbered with reference to SEQ ID NO: 194.In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: H7Q / V12I / T54S / I110V / A150D / A153L / P194E / A205M / A211L / W249Y, V12I / T54S / K72T / I110V / A150D / T152M / A153L / P194E / A205M / A211L / W249Y, V12I / K72S / R101Y / T103Q / I110V / T152M / W249Y, V12I / K72S / I110V / L147I / T152M / E204Q, E45L / T54S / K72S / I110V / T152M / P194E / E204Q, K72S / I110V / L147M / A150D / T152M / A153L / P194E / A205M / A211L / W249Y, and I110V / A150D / A153L / Y179M / P194E / A205M / A211L / W249Y, wherein the positions are numbered with reference to SEQ ID NO:194.

[0025] The present invention also provides an engineered ketoreductase variant that has at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO:252 and has at least one substitution or set of substitutions at one or more positions selected from: 7 / 12 / 54 / 179 / 249, 7 / 152, 12 / 54 / 72 / 152 / 179 / 249, 40, 54 / 72, 72 / 147 / 152 / 179 / 249, and 249, wherein the positions are numbered with reference to SEQ ID NO:252. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: 7Q / 12I / 54S / 179Y / 249Y, 7Q / 152M, 12I / 54S / 72T / 152M / 179Y / 249Y, 40E, 54S / 72S, 72S / 147M / 152M / 179Y / 249Y, and 249Y, wherein the positions are numbered with reference to SEQ ID NO:252. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: H7Q / V12I / T54S / M179Y / W249Y, H7Q / T152M, V12I / T54S / K72T / T152M / M179Y / W249Y, H40E, T54S / K72S, K72S / L147M / T152M / M179Y / W249Y, and W249Y, wherein the positions are numbered with reference to SEQ ID NO:252.

[0026] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO: 270 and have at least one substitution or set of substitutions at one or more positions selected from the following: 92 / 93, 150 / 152, 150 / 152 / 153, and 194 / 195, where the positions are numbered with reference to SEQ ID NO: 270. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: 92A / 93E, 150D / 152A / 153L, 150Y / 152A, 150Y / 152S, and 194S / 195A, where the positions are numbered with reference to SEQ ID NO: 270. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: G92A / I93E, A150D / M152A / A153L, A150Y / M152A, A150Y / M152S, and E194S / R195A, where the positions are numbered with reference to SEQ ID NO: 270.

[0027] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO: 272 and have at least one substitution or set of substitutions at one or more positions selected from the following: 92 / 93 / 95, 93, 93 / 95, 93 / 95 / 109, 93 / 95 / 109 / 114, 93 / 95 / 114, 93 / 109 / 114 and 114, where the positions are numbered with reference to SEQ ID NO: 272. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: 92A / 93D / 95R, 93A / 95K / 109R, 93A / 95R / 109D / 114T, 93D / 95R, 93E / 109R / 114A, 93M, 93R / 95A / 114T and 114A, where the positions are numbered with reference to SEQ ID NO: 272. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: G92A / I93D / V95R, I93A / V95K / K109R, I93A / V95R / K109D / N114T, I93D / V95R, I93E / K109R / N114A, I93M, I93R / V95A / N114T and N114A, where the positions are numbered with reference to SEQ ID NO: 272.

[0028] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO: 286 and have at least one substitution or set of substitutions at one or more positions selected from: 12 / 45 / 72 / 109 / 249, 12 / 45 / 93 / 249, 12 / 45 / 249, 12 / 109 / 249, 45 / 72 / 249, 45 / 109 / 249, 45 / 249, 96 and 145 / 150, wherein the positions are numbered with reference to SEQ ID NO: 286. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: 12I / 45E / 72T / 109D / 249Y, 12I / 45E / 93A / 249Y, 12I / 45E / 249Y, 12I / 109D / 249Y, 45E / 72T / 249Y, 45E / 109D / 249Y, 45E / 249Y, 96A and 145A / 150A, wherein the positions are numbered with reference to SEQ ID NO: 286. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: V12I / L45E / S72T / K109D / W249Y, V12I / L45E / I93A / W249Y, V12I / L45E / W249Y, V12I / K109D / W249Y, L45E / S72T / W249Y, L45E / K109D / W249Y, L45E / W249Y, I96A and M145A / Y150A, wherein the positions are numbered with reference to SEQ ID NO: 286.

[0029] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO: 328 and have at least one substitution or set of substitutions at one or more positions selected from the following positions: 150, 150 / 151, 150 / 195, and 195, where the positions are numbered with reference to SEQ ID NO: 328. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: 150A, 150A / 151A, 150A / 195S, 195A, and 195S, where the positions are numbered with reference to SEQ ID NO: 328. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from the following: Y150A, Y150A / P151A, Y150A / R195S, R195A, and R195S, where the positions are numbered with reference to SEQ ID NO: 328.

[0030] The present invention also provides engineered ketoreductase variants that have at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity with SEQ ID NO: 330 and have at least one substitution or set of substitutions at one or more positions selected from: 12 / 72 / 109 / 195, 17 / 73 / 200, 17 / 115, 68 / 72 / 101 / 152 / 205, 68 / 72 / 124, 68 / 72 / 124 / 152, 68 / 101 / 124 / 152 / 205, 68 / 124 / 205, 72 / 109 / 152 / 195, 72 / 109 / 195, 72 / 152, 72 / 152 / 195, 72 / 195, 73, 73 / 147, 79, 93, 93 / 95 / 145 / 195, 93 / 109 / 114 / 145 / 195, 93 / 195, 95 / 195, 96 / 108 / 147 / 200, 96 / 194 / 200, 101 / 205, 145 / 195, 147, 147 / 200, 192, 194, 194 / 200, 195, 198 and 200, where the positions are numbered with reference to SEQ ID NO: 330. In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: 12V / 72S / 109K / 195R, 17A / 73V / 200P, 17A / 115Q, 68E / 72D / 101K / 152Q / 205L, 68R / 72D / 101Q / 152Q / 205L, 68R / 72R / 124E, 68R / 72R / 124E / 152Q, 68R / 101Q / 124E / 152Q / 205L, 68R / 124E / 205L, 72D / 152Q, 72K / 152M / 195R, 72K / 195R, 72S / 109K / 152M / 195R, 72S / 109K / 195R, 73V, 73V / 147I, 79A, 93A, 93A / 95R / 145A / 195R, 93A / 109K / 114T / 145A / 195R, 93A / 195R, 95R / 195R, 96P / 108S / 147I / 200P, 96P / 194N / 200P, 101M / 205L, 145A / 195A, 147I, 147I / 200P, 192R, 194N, 194N / 200P, 195R, 198G, 198R and 200P, where the positions are numbered with reference to SEQ ID NO: 330.In some additional embodiments, the engineered ketoreductase variant comprises at least one substitution or set of substitutions selected from: I12V / T72S / D109K / S195R, M17A / L73V / E200P, M17A / L115Q, A68E / T72D / R101K / A152Q / A205L, A68R / T72D / R101Q / A152Q / A205L, A68R / T72R / L124E, A68R / T72R / L124E / A152Q, A68R / R101Q / L124E / A152Q / A205L, A68R / L124E / A205L, T72D / A152Q, T72K / A152M / S195R, T72K / S195R, T72S / D109K / A152M / S195R, T72S / D109K / S195R, L73V, L73V / L147I, E79A, I93A, I93A / V95R / M145A / S195R, I93A / D109K / N114T / M145A / S195R, I93A / S195R, V95R / S195R, I96P / R108S / L147I / E200P, I96P / E194N / E200P, R101M / A205L, M145A / S195A, L147I, L147I / E200P, K192R, E194N, E194N / E200P, S195R, Q198G, Q198R and E200P, wherein the positions are numbered with reference to SEQ ID NO: 330.

[0031] The present invention also provides engineered ketoreductase variants, which comprise a polypeptide sequence containing a sequence having at least 90% sequence identity with SEQ ID NO: 4, 6, 80, 104, 172, 186, 194, 252, 270, 272, 286, 328, and / or 330. In some embodiments, the engineered ketoreductase variant comprises a polypeptide sequence containing a sequence having at least 95% sequence identity with SEQ ID NO: 4, 6, 80, 104, 172, 186, 194, 252, 270, 272, 286, 328, and / or 330. In some additional embodiments, the engineered ketoreductase variant comprises the polypeptide sequence listed in SEQ ID NO: 4, 6, 80, 104, 172, 186, 194, 252, 270, 272, 286, 328, and / or 330. In some additional embodiments, the engineered ketoreductase variant comprises a polypeptide sequence encoding a variant provided in Table 5-1, Table 6-1, Table 7-1, Table 8-1, Table 17-2, Table 18-1, Table 19-1, Table 19-2, Table 20-1, Table 20-2, Table 21-1, Table 22-1, and / or Table 24-1. In some additional embodiments, the engineered ketoreductase variant comprises a polypeptide sequence selected from the even-numbered sequences listed in SEQ ID NO: 6 to SEQ ID NO: 412.

[0032] The present invention also provides engineered polynucleotide sequences encoding the engineered ketoreductase variants provided herein. In some embodiments, the engineered polynucleotide sequence comprises a polynucleotide sequence that is at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more identical to a sequence selected from the odd-numbered sequences listed in SEQ ID NO: 5 to SEQ ID NO: 411. The present invention also provides a vector comprising the engineered polynucleotide sequence encoding the engineered ketoreductase variant provided herein. In some embodiments, the vector further comprises at least one control sequence. In some embodiments, the vector comprises SEQ ID NO: 413 and / or 414.

[0033] The present invention also provides a host cell comprising a vector that comprises a polynucleotide encoding the engineered ketoreductase variant provided herein.

[0034] The present invention also provides methods for generating the engineered ketoreductase variants provided herein, which methods include culturing a host cell provided herein under conditions for the host cell to generate an engineered ketoreductase variant. In some embodiments, the method further includes the step of recovering the engineered ketoreductase variant produced by the host cell. In some additional embodiments, the method for generating an engineered ketoreductase variant includes culturing a host cell containing a vector of SEQ ID NO: 413 and / or 414.

[0035] The present invention also provides immobilized engineered ketoreductase variants.

[0036] The present invention also provides a composition comprising at least one engineered ketoreductase variant provided herein. In some embodiments, the composition comprises at least one immobilized engineered ketoreductase variant provided herein. Sequence Listing <110> Codexis, Inc. <120> Ketoreductase Polypeptides and Polynucleotides <130> CX8-195WO1 <140> <141> 2020-09-24 <150> US 62 / 906,268 <151> 2019-09-26 <160> 414 <210> 1 <211> 759 <212> DNA <213> Artificial Sequence <220> <223> Wild-type Kred from Lactobacillus kefir <400> 1 atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttgaggggtt cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa 600 ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtatacc gcacagtga 759 <210> 2 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Wild-type Kred from Lactobacillus kefir <400> 2 Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Glu Gly Phe Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 180 185 190 Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 245 250 <210> 3 <211> 759 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 3 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacagtga 759 <210> 4 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variants of Kred from Lactobacillus kefir <400> 4 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 5 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 5 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 6 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 6 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 7 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 7 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaggga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 8 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 8 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Arg Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 9 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variants of Kred from Lactobacillus kefir <400> 9 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgaggg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 10 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 10 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Arg Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 11 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 11 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta tttctgggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 12 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 12 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Ser Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 13 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 13 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgggtctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 14 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 14 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Gly Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 15 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 15 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggcatga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 16 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 16 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg His Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 17 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 17 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcaggggttg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 18 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 18 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Val Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 19 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 19 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 20 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 20 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 21 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 21 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttggtgggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 22 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 22 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Gly Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 23 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 23 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta tttgtgggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 24 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered Variant of Kred from Lactobacillus kefir <400> 24 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Cys Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 25 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 25 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacggttg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 26 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 26 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Val Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 27 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 27 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcag ggatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 28 <211> 252 <212> PRT <213> Artificial Sequence <220> Engineered variant of Kred from Lactobacillus kefir <400> 28 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Arg Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 29 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 29 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 agggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 30 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 30 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Arg Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 31 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 31 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcaggggatg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 32 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 32 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Asp Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 33 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 33 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cattggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 34 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 34 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Ile Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 35 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 35 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgtgtg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 36 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 36 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Cys Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 37 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered Variant of Kred from Lactobacillus kefir <400> 37 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctgcag 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 38 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 38 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Gln Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 39 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 39 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagacca ggcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 40 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 40 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Arg Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 41 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 41 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctgcgg 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 42 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 42 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Arg Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 43 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 43 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatggtgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 44 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 44 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Val Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 45 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 45 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggatgg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 46 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 46 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Met Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 47 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 47 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcgg tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 48 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 48 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Gly Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 49 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 49 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagacca atcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 50 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 50 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Asn Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 51 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 51 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctgttg 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 52 <211> 252 <212> PRT <213> Artificial Sequence <220> Engineered variants of Kred from Lactobacillus kefir <400> 52 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Leu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 53 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 53 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatt ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 54 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 54 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 55 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 55 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggctgtg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 56 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 56 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Leu Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 57 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 57 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggacgg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 58 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 58 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Thr Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 59 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 59 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaagtccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 60 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 60 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Ser Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 61 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 61 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc atcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 62 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 62 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr His Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 63 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 63 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgtctctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 64 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 64 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Ser Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 65 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 65 atgaccgatc gtctgaagca taaagtagcc atcattaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 66 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 66 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 67 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 67 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattgg gagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 68 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 68 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Gly Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 69 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 69 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgacctt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 70 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 70 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 71 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 71 atgaccgatc gtctgaagca taaagtagcc atcattaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 72 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 72 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 73 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 73 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 74 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 74 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 75 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 75 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc ctcggaaaga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 76 <211> 252 <212> PRT <213> Artificial Sequence <220> Engineered variant of Kred from Lactobacillus kefir <400> 76 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 77 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 77 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgggcctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 78 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 78 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Gly Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 79 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 79 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 80 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 80 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 81 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 81 atgaccgatc gtctgaagca taaagtagcc atcattaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgggcctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 82 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 82 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Gly Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 83 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 83 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc atcggaaaga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 84 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 84 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr His Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 85 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered Variant of Kred from Lactobacillus kefir <400> 85 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcataca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgggcctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc ctcggaaagg tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 86 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 86 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Gly Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Gly Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 87 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 87 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagacca accggaaaga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 88 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 88 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Asn Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 89 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 89 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc atcggaaaga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaacgccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 90 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 90 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr His Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 91 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 91 atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgagcctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 92 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 92 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Ser Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 93 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 93 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc ctcggaaagg tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggctgtg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 94 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 94 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Gly Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Leu Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 95 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 95 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc atcggaaaga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 96 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 96 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leucine Glycine Isoleucine Glycine Leucine Alanine Isoleucine Alanine Aspartic Acid Lysine Phenylalanine Valine Glutamic Acid Glutamic Acid Glycine Alanine 20 25 30 Lysine Valine Valine Isoleucine Threonine Glycine Arginine Histidine Alanine Aspartic Acid Valine Glycine Glutamic Acid Lysine Alanine Alanine 35 40 45 Lysine Serine Isoleucine Glycine Glycine Threonine Aspartic Acid Valine Isoleucine Arginine Phenylalanine Valine Glutamine Histidine Aspartic Acid Alanine 50 55 60 Serine Glutamic Acid Glutamic Acid Alanine Glycine Tryptophan Threonine Lysine Leucine Phenylalanine Aspartic Acid Threonine Threonine Glutamic Acid Glutamic Acid Alanine 65 70 75 80 Phenylalanine Glycine Proline Valine Threonine Threonine Leucine Valine Asparagine Asparagine Alanine Glycine Isoleucine Glycine Valine Isoleucine 85 90 95 Lysine Serine Valine Glutamic Acid Aspartic Acid Threonine Threonine Threonine Glutamic Acid Glutamic Acid Tryptophan Arginine Lysine Leucine Leucine Serine 100 105 110 Valine Asparagine Leucine Aspartic Acid Glycine Valine Phenylalanine Phenylalanine Glycine Threonine Arginine Leucine Glycine Isoleucine Glutamine Arginine 115 120 125 Methionine Lysine Asparagine Lysine Glycine Leucine Glycine Alanine Serine Isoleucine Isoleucine Asparagine Methionine Serine Serine Isoleucine 130 135 140 Methionine Glycine Leucine Valine Glycine Aspartic Acid Proline Threonine Leucine Glycine Alanine Tyrosine Asparagine Alanine Serine Lysine 145 150 155 160 Glycine Alanine Valine Arginine Isoleucine Methionine Serine Lysine Serine Alanine Alanine Leucine Aspartic Acid Cysteine Alanine Leucine 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr His Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 97 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 97 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttctggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc ctcggaaaga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 98 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 98 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 99 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 99 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg cgtaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 100 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 100 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Arg Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 101 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 101 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaag tggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 102 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 102 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Ser Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 103 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 103 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaca gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 104 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 104 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 105 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 105 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgctctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 106 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 106 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Ala Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 107 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 107 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgaagctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 108 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 108 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Lys Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 109 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 109 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 acgactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 110 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 110 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Thr Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 111 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 111 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaagtg ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 112 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 112 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 113 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 113 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 tgtactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 114 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 114 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Cys Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 115 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 115 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 116 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 116 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 117 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 117 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgattgtat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 118 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 118 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Cys Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 119 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 119 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 gggggtgtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 120 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 120 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Gly Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 121 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 121 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg ggattattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 122 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 122 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ile Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 123 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 123 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgttgg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 124 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 124 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Trp Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 125 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 125 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtttt ttaaccccta tgggccacat tggcgaaccg 660 ggttgggagg aaatgatgtc acagcgtttt ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 126 <210> 126 <211> 252 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 126 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Phe Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 127 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 127 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 gctgcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 128 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 128 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Ala Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 129 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 129 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatcgt tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 130 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 130 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Val Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 131 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 131 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaagtggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 132 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 132 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Val Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 133 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 133 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 ctgactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 134 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 134 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Leu Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 135 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 135 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 136 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 136 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 137 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 137 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgttgctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 138 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 138 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Leu Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 139 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 139 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgagttggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 140 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 140 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Leu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 141 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 141 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acggcgctgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 142 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 142 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Ala Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 143 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 143 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgct atcaagaccg agcgcaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 144 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 144 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Glu Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 145 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 145 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggccct atcaagaccg agcgcaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 146 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 146 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Glu Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 147 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 147 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatactg cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 148 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 148 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Cys Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 149 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 149 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 150 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 150 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 151 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 151 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaag tggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 152 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 152 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Ser Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 153 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 153 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggccct accaagaccg agcgcaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 154 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 154 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Thr Lys 180 185 190 Thr Glu Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 155 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 155 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgct accaagaccg agcgcaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 156 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 156 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Thr Lys 180 185 190 Thr Glu Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 157 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 157 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgctactaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 158 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 158 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Thr Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 159 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 159 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 160 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 160 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 161 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 161 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgcgctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 162 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 162 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Ala Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 163 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 163 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tgctctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 164 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 164 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Ala Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 165 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 165 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaagtagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgctctggaa 600 ggttgggagg aaatgatgtc acagcgtacg cgtaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 166 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 166 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Val Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Pro Arg Lys Asp Ala Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Arg Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 167 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 167 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaca gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaagtagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccg atcaagaccg agcggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 168 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 168 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Val Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 169 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 169 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaagtagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tgatctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 170 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 170 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Val Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 171 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 171 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 172 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 172 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 173 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 173 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaca gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaagtagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 174 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 174 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Val Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 175 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 175 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttgttaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcccg atcaagaccg agcggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 176 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 176 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Val 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 180 185 190 Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 177 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 177 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagc agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 178 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 178 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 179 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 179 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catgggtgtg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 180 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 180 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 181 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 181 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 tatactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 182 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 182 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Tyr Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 183 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 183 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggacatggat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 184 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 184 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Met Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 185 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 185 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 186 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 186 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 187 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 187 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gggtctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 188 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 188 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Gly Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 189 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 189 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 190 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 190 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 191 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 191 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtttgaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 192 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 192 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Methionine Glycine Isoleucine Glycine Leucine Alanine Isoleucine Alanine Aspartic acid Lysine Phenylalanine Valine Glutamic acid Glutamic acid Glycine Alanine 20 25 30 Lysine Valine Valine Isoleucine Threonine Glycine Arginine Histidine Alanine Aspartic acid Valine Glycine Leucine Lysine Alanine Alanine 35 40 45 Lysine Serine Isoleucine Glycine Glycine Threonine Aspartic acid Valine Isoleucine Arginine Phenylalanine Valine Glutamine Histidine Aspartic acid Alanine 50 55 60 Serine Glutamic acid Glutamic acid Alanine Glycine Tryptophan Threonine Lysine Leucine Phenylalanine Aspartic acid Threonine Threonine Glutamic acid Glutamic acid Alanine 65 70 75 80 Phenylalanine Glycine Proline Valine Threonine Threonine Leucine Valine Asparagine Asparagine Alanine Glycine Isoleucine Glycine Valine Isoleucine 85 90 95 Lysine Serine Valine Glutamic acid Aspartic acid Threonine Threonine Threonine Glutamic acid Glutamic acid Tryptophan Arginine Lysine Isoleucine Leucine Serine 100 105 110 Valine Asparagine Leucine Aspartic acid Glycine Valine Phenylalanine Phenylalanine Glycine Threonine Arginine Leucine Glycine Isoleucine Glutamine Arginine 115 120 125 Methionine Lysine Asparagine Lysine Glycine Leucine Glycine Alanine Serine Isoleucine Isoleucine Asparagine Methionine Serine Serine Isoleucine 130 135 140 Methionine Glycine Leucine Valine Glycine Aspartic acid Proline Threonine Leucine Glycine Alanine Tyrosine Asparagine Alanine Serine Lysine 145 150 155 160 Glycine Alanine Valine Arginine Isoleucine Methionine Serine Lysine Serine Alanine Alanine Leucine Aspartic acid Cysteine Alanine Leucine 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 193 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 193 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aagcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 194 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 194 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Met Ser Gln 195 200 205 Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 195 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 195 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 196 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 196 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 197 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 197 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattgc gagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 198 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 198 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 85 90 95 Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 199 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 199 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattaa gagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aagcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 200 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 200 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 85 90 95 Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Met Ser Gln 195 200 205 Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 201 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 201 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattgc gagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 202 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 202 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 203 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 203 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagctcga tcgcggggct cgtaggcgat ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 204 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 204 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Ala Gly Leu Val Gly Asp Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 205 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 205 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtagccaa gagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 206 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 206 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ala 85 90 95 Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 207 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 207 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattgc gagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aagcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 208 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 208 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Met Ser Gln 195 200 205 Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 209 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 209 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattaa gagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aagcagcttc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 210 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 210 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 85 90 95 Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Ala Ser Gln 195 200 205 Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 211 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 211 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattgc gagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagctcga tcatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aagcaatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 212 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 212 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Met Ser Gln 195 200 205 Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 213 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 213 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagctcga tcatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatggcttc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 214 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 214 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 20 25 30 Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 35 40 45 Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 50 55 60 Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 65 70 75 80 Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 85 90 95 Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 100 105 110 Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 115 120 125 Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 130 135 140 Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 145 150 155 160 Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Ala Ser Gln 195 200 205 Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 215 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 215 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aaatggcttc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg cgggtggacc gcacag 756 <210> 216 <211> 252 <212> PRT <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 216 Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 1 5 10 15 Methionine Glycine Isoleucine Glycine Leucine Alanine Isoleucine Alanine Aspartic Acid Lysine Phenylalanine Valine Glutamic Acid Glutamic Acid Glycine Alanine 20 25 30 Lysine Valine Valine Isoleucine Threonine Glycine Arginine Histidine Alanine Aspartic Acid Valine Glycine Glutamic Acid Lysine Alanine Alanine 35 40 45 Lysine Serine Isoleucine Glycine Glycine Threonine Aspartic Acid Valine Isoleucine Arginine Phenylalanine Valine Glutamine Histidine Aspartic Acid Alanine 50 55 60 Serine Glutamic Acid Glutamic Acid Alanine Glycine Tryptophan Threonine Lysine Leucine Phenylalanine Aspartic Acid Threonine Threonine Glutamic Acid Glutamic Acid Alanine 65 70 75 80 Phenylalanine Glycine Proline Valine Threonine Threonine Leucine Valine Asparagine Asparagine Alanine Glycine Isoleucine Glycine Valine Isoleucine 85 90 95 Lysine Serine Valine Glutamic Acid Arginine Threonine Threonine Threonine Glutamic Acid Glutamic Acid Tryptophan Arginine Lysine Isoleucine Leucine Serine 100 105 110 Valine Asparagine Leucine Aspartic Acid Glycine Valine Phenylalanine Phenylalanine Glycine Threonine Arginine Leucine Glycine Isoleucine Glutamine Arginine 115 120 125 Methionine Lysine Asparagine Lysine Glycine Leucine Glycine Alanine Serine Isoleucine Isoleucine Asparagine Methionine Serine Serine Isoleucine 130 135 140 Methionine Glycine Leucine Valine Glycine Alanine Proline Threonine Alanine Glycine Alanine Tyrosine Asparagine Alanine Serine Lysine 145 150 155 160 Glycine Alanine Valine Arginine Isoleucine Methionine Serine Lysine Serine Alanine Alanine Leucine Aspartic Acid Cysteine Alanine Leucine 165 170 175 Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 180 185 190 Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Ala Ser Gln 195 200 205 Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 210 215 220 Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 225 230 235 240 Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 245 250 <210> 217 <211> 756 <212> DNA <213> Artificial Sequence <220> <223> Engineered variant of Kred from Lactobacillus kefir <400> 217 atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60 ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120 gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180 cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240 ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcagccgc gagcgttgaa 300 cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360 ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420 atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480 ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540 gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600 ggttgggagg aagcaatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660 aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720 gcagaatttg tggtcgacgg ...

Claims

1. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant differs from the reference sequence SEQ ID NO: 4 by L196K, and the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO:

4.

2. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 6, or differs from SEQ ID NO: 6 by a combination of 1 - 4 substitutions selected from: V12I, V87L, L110I, L145M, T152G / S, P194H / N, D197G, I226L, and the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 6, and the engineered ketoreductase variant has improved activity in converting iso-α-acids to the corresponding dihydro-(ρ)-iso-α-acids as compared to the ketoreductase of SEQ ID NO:

4.

3. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 80, or differs from SEQ ID NO: 80 by a combination of 1 - 3 substitutions selected from: L17S / Q / M, L21A, K46V, V56C, K72A, E79L, V95I, D101T / C / L, I110V, T152K / L, N157C, S159T, A162G, P190A, I191T, P194E, D198A / Q, T210W / F, L211R, C227V, and the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 80, and the engineered ketoreductase variant has improved activity in converting iso-α-acids to the corresponding dihydro-(ρ)-iso-α-acids as compared to the ketoreductase of SEQ ID NO:

4.

4. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 104, or differs from SEQ ID NO: 104 by a combination of 3 - 4 substitutions selected from: Q17M, K46V, I96V, P190A, P194E, D198A / Q, L211R, and the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 104, and the engineered ketoreductase variant has improved activity in converting iso-α-acids to the corresponding dihydro-(ρ)-iso-α-acids as compared to the ketoreductase of SEQ ID NO:

4.

5. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 186, or is different from SEQ ID NO: 186 by a combination of 3 - 8 substitutions selected from: V95A, I96A, K97A, S143A, I144A, M145A, D150A, L153A, W202A, M205A, M206A, L211A, W249A, wherein the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 186, and wherein the engineered ketoreductase variant has improved activity in converting iso-α-acids to the corresponding dihydro-(ρ)-iso-α-acids as compared to the ketoreductase of SEQ ID NO:

4.

6. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 186, or is different from SEQ ID NO: 186 by a combination of 1 - 3 substitutions selected from: H7Q, T103R, I110V, L147I, Y179M, P194E, W249A, wherein the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 186, and wherein the engineered ketoreductase variant has improved activity in converting iso-α-acids to the corresponding dihydro-(ρ)-iso-α-acids as compared to the ketoreductase of SEQ ID NO:

4.

7. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 194, or is different from SEQ ID NO: 194 by a combination of 6 - 10 substitutions selected from: H7Q, V12I, E45L, T54S, K72S, R101Y, T103Q, I110V, L147I, A150D, T152M, A153L, P194E, E204Q, A205M, A211L, W249Y, wherein the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 194, and wherein the engineered ketoreductase variant has improved activity in converting iso-α-acids to the corresponding dihydro-(ρ)-iso-α-acids as compared to the ketoreductase of SEQ ID NO:

4.

8. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 252, or is different from SEQ ID NO: 252 by a combination of 1 - 6 substitutions selected from: H7Q, V12I, H40E, T54S, K72S / T, L147M, T152M, M179Y, W249Y, wherein the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 252, and wherein the engineered ketoreductase variant has improved activity in converting iso-α-acids to the corresponding dihydro-(ρ)-iso-α-acids as compared to the ketoreductase of SEQ ID NO:

4.

9. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 270 or is a combination of 2 - 3 substitutions selected from the following: G92A, I93E, A150Y / D, M152A / S, A153L, E194S, R195A, wherein the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 270, and wherein the engineered ketoreductase variant has improved activity in converting iso-α-acid to the corresponding dihydro-(ρ)-iso-α-acid as compared to the ketoreductase of SEQ ID NO:

4.

10. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 272, or is a combination of 1 - 4 substitutions selected from the following: G92A, I93E / M / D, V95R / K / A, K109R / D, N114T / A, wherein the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 272, and wherein the engineered ketoreductase variant has improved activity in converting iso-α-acid to the corresponding dihydro-(ρ)-iso-α-acid as compared to the ketoreductase of SEQ ID NO:

4.

11. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 286 or is a combination of 1 - 5 amino acid substitutions selected from the following: V12I, L45E, S72T, I93A, I96A, K109D, M145A, Y150A, W249Y, wherein the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 286, and wherein the engineered ketoreductase variant has improved activity in converting iso-α-acid to the corresponding dihydro-(ρ)-iso-α-acid as compared to the ketoreductase of SEQ ID NO:

4.

12. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 328 or is a combination of 1 - 2 substitutions selected from the following: Y150A, P151A, R195S / A, wherein the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 328, and wherein the engineered ketoreductase variant has improved activity in converting iso-α-acid to the corresponding dihydro-(ρ)-iso-α-acid as compared to the ketoreductase of SEQ ID NO:

4.

13. An engineered ketoreductase variant, wherein the amino acid sequence of the engineered ketoreductase variant is SEQ ID NO: 330 or a combination of 1 - 5 substitutions selected from the following differences from SEQ ID NO: 330: I12V, M17A, A68R, T72K / S / D / R, L73V, E79A, I93A, V95R, I96P, R101Q / K / M, R108S, D109K, N114T, L115Q, L124E, M145A, L147I, A152M / Q, K192R, E194N, S195R, Q198G / R, E200P, A205L, wherein the amino acid positions of the amino acid sequence are numbered with reference to SEQ ID NO: 330, and wherein the engineered ketoreductase variant comprises improved activity in converting iso-α-acids to the corresponding dihydro-(ρ)-iso-α-acids as compared to the ketoreductase of SEQ ID NO:

4.

14. The engineered ketoreductase variant according to any one of claims 1 - 13, wherein the polypeptide sequence of the engineered ketoreductase variant is listed in SEQ ID NO: 6, 80, 104, 172, 186, 194, 252, 270, 272, 286, 328 or 330.

15. The engineered ketoreductase variant according to any one of claims 1 - 13, wherein the polypeptide sequence of the engineered ketoreductase is any one of the even-numbered sequences selected from SEQ ID NO: 6 and SEQ ID NOs: 72 to 412.

16. The engineered ketoreductase variant according to claim 1, wherein the engineered ketoreductase polypeptide is capable of converting one or more iso-α-acid substrates to one or more corresponding dihydro-(ρ)-iso-α-acid products.

17. The engineered ketoreductase variant according to claim 1, wherein the engineered ketoreductase variant is capable of converting one or more iso-α-acid substrates to one or more corresponding dihydro-(ρ)-iso-α-acid products with an activity at least 10 times that of the reference polypeptide SEQ ID NO:

4.

18. The engineered ketoreductase variant according to claim 1, wherein the engineered ketoreductase variant has improved activity at high substrate concentrations as compared to the ketoreductase of SEQ ID NO:

4.

19. The engineered ketoreductase variant according to claim 1, wherein the engineered ketoreductase variant has improved activity at low cofactor concentrations as compared to the ketoreductase of SEQ ID NO:

4.

20. An engineered polynucleotide encoding the engineered ketoreductase variant according to any one of claims 1 - 19.

21. The engineered polynucleotide according to claim 20, wherein the polynucleotide sequence comprised by the engineered polynucleotide is selected from SEQ ID NO: 5 and the odd-numbered sequences among SEQ ID NOs: 71 to 411.

22. A vector, said vector comprising the engineered polynucleotide sequence according to claim 20 or 21.

23. The vector according to claim 22, said vector further comprising at least one control sequence.

24. The vector according to claim 22 or 23, wherein said vector comprises SEQ ID NO: 413 or 414.

25. A host cell, said host cell comprising the vector according to any one of claims 22, 23 or 24.

26. A method for producing the engineered ketoreductase variant according to any one of claims 1-19, said method comprising culturing the host cell according to claim 25 under conditions for producing the engineered ketoreductase variant.

27. The method according to claim 26, said method further comprising the step of recovering the engineered ketoreductase variant produced by the host cell.

28. The method according to claim 26 or claim 27, wherein the engineered ketoreductase variant is produced by a host cell containing the vector comprising SEQ ID NO: 413 or 414.

29. A composition, said composition comprising at least one engineered ketoreductase variant provided in any one of claims 1-19.

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