Portuguese Oyster Triploid Artificial Seedling Breeding Technology

Abstract

This invention relates to the field of seedling breeding technology, specifically to the artificial breeding technology of triploid Portuguese oysters. The method involves hybridizing cultivated tetraploid Pacific oysters and cultivated diploid Portuguese oysters to obtain triploid Portuguese oyster fertilized eggs, then cultivating the triploid Portuguese oyster fertilized eggs into juvenile oysters, and finally cultivating the juvenile oysters into adult triploid Portuguese oysters. This invention provides suitable temperature and salinity for the cultivation of triploid Portuguese oysters, resulting in a good survival rate of the cultivated triploid Portuguese oysters.

Description

Technical Field

[0001] This invention relates to the field of soil remediation technology, specifically to the artificial propagation technology of triploid Portuguese oyster seedlings. Background Technology

[0002] Portuguese oysters, also known as Fujian oysters, belong to the phylum Mollusca, class Bivalvia, order Pearcellales, family Ostreidae, and genus *Macrobrachium*. They are an important farmed shellfish in South China. Portuguese oysters are not only delicious and nutritious, but also possess unique health benefits and medicinal value, making them a highly nutritious seafood delicacy.

[0003] Currently, there are two main methods for obtaining triploid Portuguese oysters: direct induction and hybridization. Direct induction suffers from low success rates. Therefore, hybridization has become the primary method for obtaining triploid Portuguese oysters.

[0004] The patent application CN201410197671.1 entitled "A method for cultivating a new strain of Portuguese oyster with golden-yellow shell and rapid growth" already exists. However, the cultivation method provided results in a low survival rate of triploid Portuguese oysters, which affects the large-scale cultivation of triploid Portuguese oysters and fails to meet the needs of users.

[0005] In conclusion, the development of artificial breeding technology for triploid Portuguese oysters remains a critical issue that urgently needs to be addressed in the field of seedling breeding technology. Summary of the Invention

[0006] In view of the above-mentioned shortcomings of the existing technology, the present invention provides an artificial breeding technology for triploid Portuguese oysters. The present invention provides suitable temperature and salinity for the cultivation of triploid Portuguese oysters, and the cultivated triploid Portuguese oysters have a good survival rate.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] The artificial breeding technology of triploid Portuguese oysters involves hybridizing cultivated tetraploid Pacific oysters and cultivated diploid Portuguese oysters to obtain triploid Portuguese oyster fertilized eggs, then cultivating the triploid Portuguese oyster fertilized eggs into juvenile oysters, and finally cultivating the juvenile oysters into triploid adults of Portuguese oysters.

[0009] Furthermore, the cultivation of tetraploid Pacific oysters involves cultivating undamaged 2-year-old purebred Pacific oyster tetraploids for 3 days under temporary salinity of 16-18 and temporary rearing temperature of 24-26℃. Preferably, the temporary salinity is 17 and the temporary rearing temperature is 25℃.

[0010] Furthermore, the cultivation of diploid Portuguese oysters involves cultivating undamaged, purebred diploid Portuguese oysters aged 3 years or older for 3 days under temporary rearing conditions of salinity of 20-22 and temporary water temperature of 24-26℃. Preferably, the temporary rearing salinity is 21 and the temporary water temperature is 25℃.

[0011] Furthermore, the hybridization involves artificially crossing diploid eggs of Portuguese oysters with tetraploid sperm of Pacific oysters.

[0012] Furthermore, the aforementioned cultivation of triploid fertilized eggs of Portuguese oysters involves hatching and selecting the best triploid fertilized eggs of Portuguese oysters, followed by larval cultivation.

[0013] Furthermore, the larval cultivation involves cultivating selected triploid larvae of Portuguese oysters into juvenile oysters under conditions of salinity of 22-24 and water temperature of 28-32℃.

[0014] Preferably, the salinity is 23 and the water temperature is 30°C.

[0015] Furthermore, the process of raising juvenile oysters into triploid adults of Portuguese oysters refers to cultivating juvenile oysters into adults using conventional artificial seedling raising techniques.

[0016] Beneficial effects

[0017] Compared with known public technologies, the technical solution provided by this invention has the following beneficial effects:

[0018] This invention provides suitable temperature and salinity for the cultivation of tetraploid *Crassostrea gigas* and diploid *Crassostrea lucida*. By hybridizing diploid *Crassostrea lucida* eggs with tetraploid *Crassostrea gigas* sperm, triploid *Crassostrea lucida* fertilized eggs are obtained. These fertilized eggs are then hatched and selected to produce high-quality, robust larvae. Providing suitable salinity and temperature during the larval rearing process results in the cultivated triploid *Crassostrea lucida* exhibiting excellent survival rates, reducing cultivation costs, and producing salt-tolerant triploid *Crassostrea lucida*. This invention has broad application prospects and is worthy of promotion. Attached Figure Description

[0019] Figure 1 This is a bar chart showing the survival rate of triploid Portuguese oysters in Example 3 of this invention;

[0020] Figure 2 This is a bar chart showing the survival rate of triploid Portuguese oysters in Example 4 of this invention.

[0021] Figure 3 This is a bar chart showing the survival rate of triploid Portuguese oysters in Example 5 of this invention. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0023] The present invention will be further described below with reference to embodiments.

[0024] Example 1: Cultivation of tetraploid Pacific oysters and diploid Portuguese oysters

[0025] (1) Cultivation of tetraploid oysters

[0026] Take undamaged 2-year-old purebred Pacific oyster tetraploids with a size of 6-10cm, and select male individuals from them. Place the male individuals in a culture tank and provide them with temporary salinity of 16-18 and temporary rearing temperature of 24-26℃. During the rearing period, feed them with unicellular algae every day according to conventional rearing methods, with a daily feed amount of 400,000-600,000 algae / ml.

[0027] (2) Cultivation of diploid Portuguese oysters

[0028] Female individuals were selected from undamaged, purebred Portuguese oysters aged 3 years and older diploids. Female individuals with a size of 7-8 cm were then selected and placed in a culture tank. They were cultured under temporary rearing conditions of salinity of 20-22 and temporary water temperature of 24-26℃. During the culture period, unicellular algae were fed daily according to conventional culture methods, with a daily feed amount of 400,000-600,000 algae per ml.

[0029] Example 2: Obtaining the triploid Portuguese oyster of the present invention

[0030] (1) Using artificial dissection, eggs were taken from diploid female individuals of cultivated Portuguese oysters and filtered through 100-mesh and 200-mesh silk sieves respectively, and then filtered through 500-mesh silk sieves. Sperm were taken from tetraploid male individuals of Pacific oysters and filtered through 500-mesh silk sieves.

[0031] (2) Observe the sperm under a microscope, select the active sperm, add the sperm to the egg fluid, and ensure that the ratio of female to male is 30:1. Mix the sperm and egg to complete the hybridization of egg and sperm, that is, complete the fertilization of egg.

[0032] (3) The fertilized eggs are hatched in 20-24 hours to form D-shaped larvae. The larvae are selected by using 300-mesh silk screen to obtain high-quality and robust larvae.

[0033] (4) The selected larvae were raised into juvenile shellfish under conditions of salinity of 22-24 and water temperature of 28-32℃;

[0034] (5) Following conventional artificial seedling cultivation procedures, the juvenile oysters are introduced into the sea and cultivated to adulthood to obtain the triploid Portuguese oyster of this invention.

[0035] Example 3: Effects of different temperatures on triploid culture of Portuguese oysters

[0036] The tetraploid oysters of the Pacific oyster were cultured at a salinity of 17 and a temperature of 25°C, while the diploid oysters of the Portuguese oyster were cultured at a salinity of 21 and a temperature of 25°C. Then, 1000 triploid Portuguese oysters were cultured at a salinity of 23 and a water temperature of 30°C, following the cultivation methods of Examples 1 and 2, as the experimental group. With other conditions unchanged, 1000 triploid Portuguese oysters were cultured at the tetraploid oysters of the Pacific oyster at temperatures of 20°C and 28°C, respectively, as control groups 1 and 2. With other conditions unchanged, the diploid Portuguese oysters were cultured at temperatures of 22°C and 28°C, respectively, as control groups 1 and 2. 1000 triploid Portuguese oysters were cultured at 28℃, serving as control groups 3 and 4. Under otherwise identical conditions, the temporary rearing temperature for tetraploid Pacific oysters was 20℃, and for diploid Portuguese oysters, it was 22℃. Additionally, 1000 triploid Portuguese oyster larvae were cultured at water temperatures of 26℃ and 34℃, serving as control groups 5 and 6. The triploid Portuguese oysters in each group were cultured using conventional artificial seedling raising methods, and the juveniles were released into the sea to grow to adulthood. The survival rate of the triploid Portuguese oysters in each group was statistically analyzed, and the relevant data are recorded in Table 1. A bar chart was also presented. Figure 1 .

[0037] Table 1. Survival rate statistics of triploid Portuguese oysters

[0038]

[0039] From Table 1 and Figure 1 It can be seen that the temperature provided by the present invention significantly improves the survival rate of triploid Portuguese oysters compared to control groups 1, 2, 3 and 4. By providing suitable temperatures for the cultivation of tetraploid Portuguese oysters, diploid Portuguese oysters and larval cultivation, the survival rate of triploid Portuguese oysters can be effectively improved to 98%.

[0040] Example 4: Effects of different salinities on triploid culture of Portuguese oysters

[0041] The tetraploid oysters of the Pacific oyster were cultured at a salinity of 17 and a temperature of 25°C, while the diploid oysters of the Portuguese oyster were cultured at a salinity of 21 and a temperature of 25°C. Then, 1000 triploid Portuguese oysters were cultured at a salinity of 23 and a temperature of 30°C, following the cultivation methods of Examples 1 and 2, as the experimental group. Under the same conditions, 1000 triploid Portuguese oysters were cultured from the tetraploid Pacific oysters at salinities of 14 and 20, respectively, as control groups 1 and 2. Under the same conditions, the diploid Portuguese oysters were cultured at a salinity of 18... 1000 triploid Portuguese oysters were cultured at salinities of 24 and 24, serving as control groups 3 and 4. Under otherwise identical conditions, the temporary rearing salinity for tetraploid Pacific oysters was 14, and for diploid Portuguese oysters, it was 18. 1000 triploid Portuguese oyster larvae were cultured at salinities of 20 and 26, serving as control groups 5 and 6. The triploid Portuguese oysters in each group were cultured using conventional artificial seedling methods, and the juveniles were released into the sea to grow to adulthood. The survival rate of the triploid Portuguese oysters in each group was statistically analyzed, and the relevant data are recorded in Table 2. A bar chart was also presented. Figure 2 .

[0042] Table 2. Survival rate statistics of triploid Portuguese oysters.

[0043]

[0044] From Table 2 and Figure 2 It can be seen that the salinity provided by this invention significantly improves the survival rate of triploid Portuguese oysters compared to control groups 1, 2, 3, and 4. By providing suitable salinity for the cultivation of tetraploid Pacific oysters, diploid Portuguese oysters, and larval stages, the survival rate of triploid Portuguese oysters can be effectively improved, reaching 92%.

[0045] Example 5: Comparison of the cultivation method of the present invention with existing technical methods

[0046] Using the method of this invention, 1000 triploid Portuguese oysters were cultivated as the experimental group, and using the method of patent application number CN201410197671.1, 1000 triploid Portuguese oysters were cultivated as the control group. The survival rate of the triploid Portuguese oysters was statistically analyzed, and the relevant data are recorded in Table 3. A bar chart was also created to illustrate the statistical results. Figure 3 .

[0047] Table 3. Survival rate data of triploid Portuguese oysters.

[0048] experimental group control group Survival rate (%) 96 85

[0049] Depend on Figure 3 As shown in Table 3, compared with traditional cultivation methods, the method of the present invention can significantly improve the survival rate of triploid Portuguese oyster breeding, thereby reducing cultivation costs and enabling the cultivated triploid Portuguese oysters to have a good salt tolerance survival rate.

[0050] This invention provides suitable temperature and salinity for the cultivation of tetraploid *Crassostrea gigas* and diploid *Crassostrea lucida*. By hybridizing diploid *Crassostrea lucida* eggs with tetraploid *Crassostrea gigas* sperm, triploid *Crassostrea lucida* fertilized eggs are obtained. These fertilized eggs are then hatched and selected to produce high-quality, robust larvae. Providing suitable salinity and temperature during the larval rearing process results in the cultivated triploid *Crassostrea lucida* exhibiting excellent survival rates, reducing cultivation costs, and producing salt-tolerant triploid *Crassostrea lucida*. This invention has broad application prospects and is worthy of promotion.

[0051] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for artificially propagating triploid Portuguese oyster seedlings, characterized in that, The method involves hybridizing cultivated tetraploid oysters and cultivated diploid oysters to obtain triploid fertilized eggs of oysters ... The cultivation of tetraploid oysters involves cultivating undamaged 2-year-old purebred tetraploid oysters under temporary rearing conditions of salinity of 16-18 and temporary rearing temperature of 24-26℃. The cultivation of diploid Portuguese oysters involves cultivating undamaged, purebred diploid Portuguese oysters aged 3 years or older under conditions of temporary rearing salinity of 20-22 and temporary rearing water temperature of 24-26℃. The hybridization described involves artificially crossing diploid eggs of Portuguese oysters with tetraploid sperm of Pacific oysters. The aforementioned Portuguese oyster triploid fertilized egg cultivation involves hatching and selecting superior Portuguese oyster triploid fertilized eggs, followed by larval cultivation. The larval cultivation involves raising selected triploid larvae of Portuguese oysters into juvenile oysters under conditions of salinity of 22-24 and water temperature of 28-32℃.

2. The method for artificial propagation of triploid Portuguese oyster seedlings according to claim 1, characterized in that, The larvae were raised under conditions of salinity of 23 and water temperature of 30°C.

3. The method for artificial propagation of triploid Portuguese oyster seedlings according to claim 1, characterized in that, The process of cultivating juvenile oysters into triploid adults of Portuguese oysters refers to cultivating juvenile oysters into adults using conventional artificial seedling cultivation techniques.

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