Manual multi-point punching sampling device for biological fluid filter membrane

Abstract

The present invention discloses a manual multi-point punching sampling device for biological fluid filter membrane, comprising a bottom plate, a hole plate moving device and a support frame arranged on the bottom plate, a hole plate arranged on the hole plate moving device, the hole plate being moved horizontally along the X-axis and the Z-axis by the hole plate moving device, a concave film being arranged on the support frame, the biological fluid filter membrane multi-point moving device being placed in the concave film, the biological fluid filter membrane multi-point moving device being pluggable connected to the support frame through a first elastic bolt, a punching device being arranged on the top of the support frame, the punching device pressing the biological fluid filter membrane sample of the biological fluid filter membrane multi-point moving device downward, an electrostatic eliminator being arranged on the support frame, the electrostatic eliminator being communicated with the concave film, the electrostatic eliminator shearing the biological fluid filter membrane sample, the present invention solves the problems of uniformity and rationality of filter membrane sampling, and is efficient and convenient.

Description

Technical Field

[0001] The invention relates to a manual multi-point punching sampling device for biological fluid filter membrane, wherein the biological fluid specifically includes urine, saliva, human milk, blood and other liquids. Background Art

[0002] Biological sample fluid contains a wealth of information about the patient, among which proteins are believed to contain potential markers for kidney disease and are considered to include information about the entire body. However, biological samples in current medical practice are not fully preserved. The high preservation cost and high technical difficulty have prevented researchers from preserving large quantities of biological sample fluid. In recent years, researchers have tried to use filter membrane adsorption, which can be dried and preserved for a long time. When the sample needs to be analyzed, the corresponding filter membrane can be called out for membrane cutting analysis, which can greatly reduce the preservation cost.

[0003] There are two main types of punching samplers currently used in the field of laboratory instrument technology: one is manual, with low sampling efficiency, and it is difficult to put the electrostatically charged filter membrane samples into the collection holes of the orifice plate; the other is automated punching sampling, which has high automated sampling efficiency and the filter membrane samples are easy to fall into the collection holes after electrostatic elimination treatment, but it is not possible to achieve uniform sampling at multiple points on the circular filter membrane. Summary of the invention

[0004] The present invention provides a manual multi-point punching sampling device for biological fluid filter membranes, which can realize continuous and uniform sampling of filter membrane samples at multiple points on the biological fluid filter membrane. The biological fluid filter membrane can be extracted and sampled multiple times for use. The sampling efficiency is high, the uniformity is good, and the representativeness is good. The sampling points can be set arbitrarily, and the biological fluid filter membrane can be reused.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is:

[0006] A manual multi-point punching sampling device for biological fluid filter membrane, characterized in that: a hole plate moving device and a support frame are arranged on the bottom plate;

[0007] The orifice plate can be plugged and unplugged on the orifice plate moving device, and the orifice plate is horizontally moved along the X-axis and the Z-axis by the orifice plate moving device to adjust the relative position of the orifice plate and the support frame;

[0008] A concave membrane is arranged on the support frame, and a biological fluid filter membrane multi-point moving device capable of radial rotation is placed in the concave membrane. The biological fluid filter membrane multi-point moving device is also pluggably connected to the support frame through a first elastic bolt. A punching device is arranged on the top of the support frame, and the punching device presses down the biological fluid filter membrane sample of the biological fluid filter membrane multi-point moving device through telescopic movement. A static eliminator is arranged on the support frame, and the static eliminator is communicated with the concave membrane. The static eliminator shears the biological fluid filter membrane sample through airflow, and the biological fluid filter membrane sample can fall into the collection hole in the orifice plate.

[0009] The manual multi-point punching sampling device for biological fluid filter membrane, wherein: the orifice plate moving device includes a Z-axis screw bearing seat, a Z-axis guide rail mounting seat, a plate seat and a collection device;

[0010] A driving gear and a passive gear are arranged on the Z-axis screw bearing seat and are meshed and fixed. The passive gear is transmission-connected to the Z-axis screw nut through the Z-axis screw. The Z-axis screw nut is fixedly connected to the plate seat. A pointer is arranged on the plate seat.

[0011] A Z-axis linear guide is arranged on the Z-axis guide mounting seat, the plate seat is arranged on the slideway of the Z-axis linear guide, the plate seat is arranged on the X-axis linear guide, the collecting device is slidably arranged on the X-axis linear guide, the collecting device is pluggably fixed to the conversion plate through a second elastic bolt, the conversion plate is pluggably fixed to the orifice plate through a left positioning plate and a right positioning plate, and a plurality of collecting holes are arranged on the orifice plate.

[0012] The manual multi-point punching sampling device for biological fluid filter membrane, wherein: the support frame includes a right vertical force arm and a left vertical force arm, the right vertical force arm and the left vertical force arm are fixedly connected by an acrylic guard plate, a punching cylinder seat is arranged above the acrylic guard plate, the punching device is arranged on the punching cylinder seat, a concave film mounting plate is arranged below the acrylic guard plate, and the concave film is arranged on the concave film mounting plate.

[0013] The manual multi-point punching sampling device for biological fluid filter membrane, wherein: the biological fluid filter membrane multi-point moving device includes a biological fluid filter membrane seat, a biological fluid filter membrane rotating seat and the biological fluid filter membrane sample, the biological fluid filter membrane rotating seat is arranged on the biological fluid filter membrane seat, the biological fluid filter membrane sample is arranged in the biological fluid filter membrane rotating seat, and the biological fluid filter membrane rotating seat drives the biological fluid filter membrane sample to move radially by rotating.

[0014] The manual multi-point punching sampling device for biological fluid filter membrane, wherein: the punching device includes a cylinder, a punching concave membrane seat and a guide pressure sleeve, the cylinder is connected to the guide pressure sleeve through the punching concave membrane seat, a punching concave membrane and a spring are arranged in the guide pressure sleeve, the spring is sleeved on the punching concave membrane, and the top of the spring is fixedly connected to the guide pressure sleeve by a positioning screw.

[0015] The manual multi-point punching sampling device for biological fluid filter membrane, wherein: the static eliminator includes a static eliminator and a guide tube, the static eliminator is fixedly connected to the support frame through the static eliminator mounting plate, the static eliminator is connected to the guide tube through a quick-connect plug, the guide tube is connected to the concave membrane, the air inlet at the top of the static eliminator is for air flow to enter, and the air flow with ions is discharged through the air outlet of the guide tube.

[0016] The manual multi-point punching sampling device for biological fluid filter membrane, wherein: a filter membrane sample drop outlet is set through the concave membrane, the bottom end of the filter membrane sample drop outlet is connected to the filter membrane sample shearing opening, and an ion air inlet and an exhaust port are set through the side wall of the filter membrane sample drop outlet, and the ion air inlet is connected to the static elimination device.

[0017] The beneficial effects of the present invention are as follows: continuous and uniform sampling of filter membrane samples at multiple points on the biological fluid filter membrane can be achieved, the biological fluid filter membrane can be used for multiple extraction and sampling, the sampling efficiency is high, the uniformity is good, the representativeness is good, the sampling points can be set arbitrarily, and the biological fluid filter membrane can be reused. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The present invention is a structural diagram of a manual multi-point punching sampling device for biological fluid filter membranes.

[0019] Figure 2 The present invention is a structural diagram of a hole plate moving device for a manual multi-point punching sampling device for biological fluid filter membranes.

[0020] Figure 3 This is a structural diagram of a conversion plate for a manual multi-point punching sampling device for biological fluid filter membranes.

[0021] Figure 4 This is a structural diagram of a support frame for a manual multi-point punching sampling device for biological fluid filter membranes.

[0022] Figure 5 The present invention is a structural diagram of a biological fluid filter membrane multi-point moving device used for a biological fluid filter membrane manual multi-point punching sampling device.

[0023] Figure 6 The present invention is a structural diagram of a punching device for a manual multi-point punching sampling device for biological fluid filter membranes.

[0024] Figure 7 This is a structural diagram of the static eliminator used in the manual multi-point punching sampling device for biological fluid filter membranes.

[0025] Figure 8 This is a diagram of the concave membrane structure of a manual multi-point punching sampling device for biological fluid filter membranes.

[0026] Explanation of the reference numerals: 1-right vertical force arm; 2-left vertical force arm; 3-punched cylinder seat; 4-concave membrane mounting plate; 5-concave membrane; 51-ion air inlet; 52-filter membrane sample cutting notch; 53-exhaust port; 54-filter membrane sample drop port; 6-acrylic guard plate; 7-first elastic pin; 8-speed regulating valve; 9-pressure regulating valve; 10-biological fluid filter membrane seat; 11-biological fluid filter membrane rotating seat; 12-biological fluid filter membrane sample; 13-cylinder; 14-punched concave membrane seat; 15-guide pressure sleeve; 16-punched concave membrane; 17-spring; 18-positioning screw; 19-static elimination 1. Device mounting plate; 20. Electrostatic eliminator; 21. Quick-connect plug; 22. Guide tube; 23. Bottom plate; 24. Rubber foot; 25. Z-axis screw bearing seat; 26. Driving gear; 7. Passive gear; 28. Z-axis screw; 29. ​​Z-axis screw nut; 30. Z-axis guide rail mounting plate; 31. Z-axis linear guide; 32. Plate seat; 33. Pointer; 34. Collection device; 35. Second elastic bolt; 36. X-axis linear guide; 39. Collection hole; 40. Conversion plate; 41. Left positioning plate; 42. Right positioning plate; 43. Orifice plate; 44. Air inlet; 45. Air outlet. DETAILED DESCRIPTION

[0027] like Figure 1 As shown, the manual multi-point punching sampling device for biological fluid filter membrane provided by the present invention has a bottom plate 23, and a hole plate moving device and a support frame are arranged on the bottom plate 23;

[0028] like Figure 2 , Figure 3 As shown, the orifice plate moving device includes a Z-axis screw bearing seat 25, a Z-axis guide rail mounting seat 30, a plate seat 32 and a collection device 34;

[0029] The Z-axis screw bearing seat 25 is arranged on the base plate 23, and a driving gear 26 and a passive gear 27 are arranged on the Z-axis screw bearing seat 25, and are meshed and fixed. The passive gear 27 can be driven to rotate synchronously by rotating the driving gear 26. The passive gear 27 is transmission-connected with the Z-axis screw nut 29 through the Z-axis screw 28. The Z-axis screw nut 29 is fixedly connected with the plate seat 32. A Z-axis linear guide 31 is arranged on the Z-axis guide rail mounting seat 30, and the plate seat 32 is arranged on the slideway of the Z-axis linear guide rail 31. The driving gear 26 rotates one circle, and at the same time drives the passive gear 27 and the Z-axis screw 28 to rotate together, and pushes the Z-axis screw nut 29 and the plate seat 32 to move 1 hole pitch along the Z-axis direction.

[0030] A pointer 33 is arranged on the plate seat 32, and an X-axis linear guide 36 is arranged on the plate seat 32. The collecting device 34 is slidably arranged on the X-axis linear guide 36. The collecting device 34 is pushed to move one scale according to the pointer 33, so that the collecting device 34 can move one hole distance along the X-axis. The collecting device 34 is pluggable and fixed to the conversion plate 40 through the second elastic bolt 35. The conversion plate 40 is pluggable and fixed to the orifice plate 43 through the left positioning plate 41 and the right positioning plate 42. A plurality of collecting holes 39 are arranged on the orifice plate 43.

[0031] Figure 4 As shown, the support frame includes a right vertical force arm 1 and a left vertical force arm 2, the right vertical force arm 1 and the left vertical force arm 2 are fixedly connected by an acrylic guard plate 6, the top of the support frame is fixedly connected to the punching device through a punching cylinder seat 3, a concave film mounting plate 4 is arranged below the punching cylinder seat 3, a red scale line is arranged on the concave film mounting plate 4, the concave film 5 is placed in the concave film mounting plate 4, a biological fluid filter membrane multi-point moving device capable of radial rotation is placed in the concave film 5, and the biological fluid filter membrane multi-point moving device is also arranged through a first The elastic bolt 7 is pluggably connected to the support frame to adjust the position of the biological fluid filter membrane multi-point moving device. The support frame is provided with an electrostatic eliminator, which is connected to the concave membrane 5. The punching device presses the biological fluid filter membrane sample 12 of the biological fluid filter membrane multi-point moving device downward through telescopic movement. The electrostatic eliminator shears the biological fluid filter membrane sample 12 that has been pressed downward through airflow, and the sheared biological fluid filter membrane sample 12 can fall into the collection hole 39 in the orifice plate 43.

[0032] Figure 5As shown, the biological fluid filter membrane multi-point moving device includes a biological fluid filter membrane seat 10, a biological fluid filter membrane rotating seat 11 and the biological fluid filter membrane sample 12. Three positioning holes are symmetrically arranged on the side wall of the biological fluid filter membrane seat 10. The biological fluid filter membrane seat 10 can adjust three insertion distances through the positioning holes. The biological fluid filter membrane seat 10 is provided with the biological fluid filter membrane rotating seat 11. The biological fluid filter membrane rotating seat 11 is provided with a scale every 120 degrees in the radial direction. 1 is provided with the biological fluid filter membrane sample 12, and the biological fluid filter membrane rotator 11 drives the biological fluid filter membrane sample 12 to move radially by rotating on the biological fluid filter membrane rotator 11. The biological fluid filter membrane rotator 11 rotates 120 degrees, so that the biological fluid filter membrane rotator 11 can be aligned with the scale of the biological fluid filter membrane seat 10, and three 4mm samples can be punched out on the biological fluid filter membrane sample 12 by rotating twice and fall into the same collection hole 39, thereby ensuring the uniformity and rationality of sampling.

[0033] Figure 6 As shown, the punching device includes a cylinder 13, a punching concave film seat 14 and a guide pressing sleeve 15, the cylinder 13 is connected to the guide pressing sleeve 15 through the punching concave film seat 14, a punching concave film 16 and a spring 17 are arranged in the guide pressing sleeve 15, the spring 17 is sleeved on the punching concave film 16, and the top of the spring 17 is fixedly connected to the guide pressing sleeve 15 through a positioning screw 18, after the cylinder 13 is ventilated, the punching concave film seat 14 and the guide pressing sleeve 15 are pushed out, under the action of the spring 17, the guide pressing sleeve 15 first presses the biological fluid filter membrane sample 12 to prevent it from moving at the initial position, at this time, the guide pressing sleeve 15 stops moving, the punching punch 16 continues to move downward and contacts the concave die 5, and the required filter membrane sample is punched off from the biological fluid filter membrane sample 12 by shearing action, and the biological fluid on the biological fluid filter membrane sample 12 specifically includes urine, saliva, human milk, blood and other liquids.

[0034] Figure 7 As shown, the static eliminator includes a static eliminator 20 and a guide tube 22. The static eliminator 20 is fixedly connected to the support frame through the static eliminator mounting plate 19. The static eliminator 20 is connected to the guide tube 22 through a quick-connect plug 21. The guide tube 22 is connected to the concave film 5. The air inlet 44 at the top of the static eliminator 20 is used for airflow to enter, and the airflow with ions is discharged through the air outlet 45 of the guide tube 22 and input into the concave mold 5 to form a shearing effect.

[0035] Figure 8As shown, a filter membrane sample drop port 54 is provided through the concave membrane 5, the bottom end of the filter membrane sample drop port 54 is communicated with the filter membrane sample cut port 52, an ion inlet 51 and an exhaust port 53 are provided through the side wall of the filter membrane sample drop port 54, and the ion inlet 51 is communicated with the guide tube 22.

[0036] In the embodiment, the driving gear 26 rotates one circle, and the driving gear 26 drives the driven gear 27 and the Z-axis lead screw 28 to rotate together, and pushes the Z-axis lead screw nut 29 and the plate seat 32 to move one hole pitch along the Z-axis direction. By manually pushing the collection device 34 to move one scale according to the pointer 33, the collection device 34 can be moved one hole pitch along the X-axis, so that the preset collection hole 39 is aligned with the center of the die 5, and the cylinder 13 is started, and the punching punch 16 continues to move downward to contact the die 5. The hole punch 16 presses down the required filter membrane sample on the biological fluid filter membrane sample 12, and starts the static eliminator 20, which can input the airflow with ions into the die 5, shearing the pressed filter membrane sample, and the filter membrane sample accurately falls into the collecting hole 39, realizing the shearing and collection of the filter membrane sample. By rotating the biological fluid filter membrane turntable 11 twice, three 4mm filter membrane samples are sheared on the biological fluid filter membrane sample 12 and fall into the same collecting hole 39, ensuring the uniformity and rationality of sampling.

[0037] The advantages of the present invention are that it can realize continuous and uniform sampling of filter membrane samples at multiple points on the biological fluid filter membrane, the biological fluid filter membrane can be extracted and sampled multiple times, the sampling efficiency is high, the uniformity is good, the representativeness is good, the sampling points can be set arbitrarily, and the biological fluid filter membrane can be reused.

[0038] The above description is only illustrative rather than restrictive of the present invention. Those skilled in the art will understand that many modifications, changes or equivalents may be made without departing from the spirit and scope defined by the claims, but all will fall within the scope of protection of the present invention.

Claims

1. A manual multi-point punching sampling device for biological fluid filter membrane, characterized in that: The bottom plate (23) is provided with an orifice plate moving device and a supporting frame; The orifice plate (43) can be plugged in and out of the orifice plate moving device, and the orifice plate (43) is horizontally moved along the X-axis and the Z-axis by the orifice plate moving device to adjust the relative position of the orifice plate (43) and the support frame; The support frame is provided with a concave membrane (5), and a biological fluid filter membrane multi-point moving device capable of radial rotation is placed in the concave membrane (5). The biological fluid filter membrane multi-point moving device is also pluggably connected to the support frame through a first elastic bolt (7). A punching device is provided at the top of the support frame, and the punching device presses the biological fluid filter membrane sample (12) of the biological fluid filter membrane multi-point moving device downward through telescopic movement. A static eliminator is provided on the support frame, and the static eliminator is connected to the concave membrane (5). The static eliminator shears the biological fluid filter membrane sample (12) through airflow, and the biological fluid filter membrane sample (12) can fall into the collection hole (39) in the orifice plate (43); The biological fluid filter membrane multi-point moving device comprises a biological fluid filter membrane seat (10), a biological fluid filter membrane rotating seat (11) and the biological fluid filter membrane sample (12); the biological fluid filter membrane rotating seat (11) is arranged on the biological fluid filter membrane seat (10), the biological fluid filter membrane sample (12) is arranged in the biological fluid filter membrane rotating seat (11), and the biological fluid filter membrane rotating seat (11) drives the biological fluid filter membrane sample (12) to move radially by rotating; A filter membrane sample drop opening (54) is provided through the concave membrane (5), the bottom end of the filter membrane sample drop opening (54) is connected to the filter membrane sample cut opening (52), an ion inlet (51) and an exhaust port (53) are provided through the side wall of the filter membrane sample drop opening (54), and the ion inlet (51) is connected to the static elimination device.

2. The manual multi-point punching sampling device for biological fluid filter membrane according to claim 1, characterized in that: The orifice plate moving device comprises a Z-axis screw rod bearing seat (25), a Z-axis guide rail mounting seat (30), a plate seat (32) and a collection device (34); A driving gear (26) and a driven gear (27) are arranged on the Z-axis screw bearing seat (25) and are meshed and fixed; the driven gear (27) is transmission-connected to a Z-axis screw nut (29) via a Z-axis screw (28); the Z-axis screw nut (29) is fixedly connected to the plate seat (32); and a pointer (33) is arranged on the plate seat (32); The Z-axis linear guide (31) is arranged on the Z-axis linear guide (31), the plate seat (32) is arranged on a slideway on the Z-axis linear guide (31), the plate seat (32) is arranged on the X-axis linear guide (36), the collection device (34) is slidably arranged on the X-axis linear guide (36), the collection device (34) is pluggably fixed to the conversion plate (40) through a second elastic bolt (35), the conversion plate (40) is pluggably fixed to the orifice plate (43) through a left positioning plate (41) and a right positioning plate (42), and a plurality of the collecting holes (39) are arranged on the orifice plate (43).

3. The manual multi-point punching sampling device for biological fluid filter membrane according to claim 1, characterized in that: The support frame comprises a right vertical force arm (1) and a left vertical force arm (2); the right vertical force arm (1) and the left vertical force arm (2) are fixedly connected via an acrylic guard plate (6); a punching cylinder seat (3) is arranged above the acrylic guard plate (6); the punching device is arranged on the punching cylinder seat (3); a concave film mounting plate (4) is arranged below the acrylic guard plate (6); the concave film (5) is arranged on the concave film mounting plate (4).

4. The manual multi-point punching sampling device for biological fluid filter membrane according to claim 1, characterized in that: The punching device comprises a cylinder (13), a punching concave film seat (14) and a guide sleeve (15); the cylinder (13) is connected to the guide sleeve (15) through the punching concave film seat (14); a punching concave film (16) and a spring (17) are arranged in the guide sleeve (15); the spring (17) is sleeved on the punching concave film (16); the top end of the spring (17) is fixedly connected to the guide sleeve (15) through a positioning screw (18).

5. The manual multi-point punching sampling device for biological fluid filter membrane according to claim 1, characterized in that: The static eliminator device comprises a static eliminator (20) and a flow guide tube (22), wherein the static eliminator (20) is fixedly connected to the support frame via a static eliminator mounting plate (19), the static eliminator (20) is connected to the flow guide tube (22) via a quick-connect plug (21), the flow guide tube (22) is connected to the concave membrane (5), an air inlet (44) at the top of the static eliminator (20) is used for airflow, and an air outlet (45) of the flow guide tube (22) is used to discharge airflow carrying ions.

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