Helical-ridge artificial blood vessel and method for manufacturing the same

Abstract

The application discloses a spiral ridge artificial blood vessel preparation method, a plurality of metal wires are tightly surrounded outside a cylindrical mandrel to form a spiral ridge collector, the spiral ridge collector is fixed on an electrostatic spinning machine, and a spiral ridge artificial blood vessel is prepared by an electrostatic spinning method; the spiral ridge artificial blood vessel has a plurality of spiral ridges on an inner wall, can induce blood to generate a spiral flow, can improve blood flow speed near a wall surface and wall surface shear stress, and can reduce deposition of harmful substances on the inner wall of the blood vessel, and has important significance for reducing re-stenosis after small-diameter artificial blood vessel transplantation and improving long-term patency. The application further provides a spiral ridge artificial blood vessel prepared by the spiral ridge artificial blood vessel preparation method.

Description

TECHNICAL FIELD

[0001] The present application relates to a spiral ridge artificial blood vessel and a preparation method thereof. BACKGROUND

[0002] With the research and development of artificial blood vessels, artificial blood vessels with spiral ridges on the inner wall are increasingly valued, which is mainly based on the theory of rotational flow in the human body. Studies have shown that blood is more likely to form rotational flow in artificial blood vessels with spiral ridges on the inner wall. Rotational flow can eliminate vortex zones, separation zones, and blood flow turbulence zones, making blood flow more stable while increasing the near-wall blood flow velocity and wall shear stress, thereby inhibiting intimal hyperplasia, thrombus adhesion, etc., which is of great significance to reducing restenosis after small-diameter artificial blood vessel transplantation and improving long-term patency. SUMMARY

[0003] The purpose of the present application is to provide a preparation method of a spiral ridge artificial blood vessel to prepare an artificial blood vessel with spiral ridges on the inner wall, in which blood is easy to form rotational flow.

[0004] One technical solution adopted by the present application is to provide a preparation method of a spiral ridge artificial blood vessel for preparing a spiral ridge artificial blood vessel with a diameter of D, comprising the following steps:

[0005] S1. Spiral ridge collector preparation:

[0006] (1) providing a cylindrical mandrel and n bendable cylindrical metal wires, the diameter of the mandrel is D1, and the diameter of the metal wires is D2, wherein n is an integer greater than or equal to 3, and D1 = D2 / sin(180° / n)-D2;

[0007] (2) arranging the n metal wires in parallel to the mandrel and surrounding the mandrel outer periphery, and connecting the first ends of the n metal wires to the mandrel;

[0008] (3) rotating the second ends of the n metal wires opposite to the first ends along the mandrel outer periphery in a predetermined direction by a predetermined angle synchronously, so that the n metal wires are tightly surrounded on the mandrel outer periphery in the form of spiral ridges;

[0009] (4) connecting the second ends of the n metal wires to the mandrel to obtain a spiral ridge collector;

[0010] S2. Electrospinning liquid configuration:

[0011] An electrospinning liquid solution with a concentration of 8% to 12% is prepared by taking poly-L-lactide-caprolactone (PLCL) or polyurethane (PU) as the solute and taking hexafluoroisopropanol, trichloromethane or tetrahydrofuran as the solvent;

[0012] S3. Spiral ridge artificial blood vessel forming:

[0013] Mounting the spiral ridge collector on an electrospinning machine, injecting the electrospinning solution into the electrospinning machine for electrospinning, and forming an electrospinning layer with a predetermined thickness on the circumferential outside of the spiral ridge collector;

[0014] S4. Demolding:

[0015] Taking down the spiral ridge collector from the electrospinning machine, separating the electrospinning layer from the spiral ridge collector, and obtaining the spiral ridge artificial blood vessel.

[0016] As an improvement to the above scheme, in step S1, the predetermined direction is clockwise or counterclockwise, and the predetermined angle is 30°-60°.

[0017] As an improvement to the above scheme, D is set to 4-9 mm, in step S1, D1 is set to 2-5 mm, and D2 is set to 1-2 mm.

[0018] As an improvement to the above scheme, the length of the metal wire is not less than the length of the spiral ridge artificial blood vessel, and the length of the mandrel is 15%-20% greater than the length of the metal wire.

[0019] As an improvement to the above scheme, in step S1, the first end and the second end of the metal wire are fixedly connected to the mandrel by buckling, and a predetermined distance is provided between the first end and the second end of the metal wire and the corresponding end of the mandrel.

[0020] As an improvement to the above scheme, in step S3, the electrospinning solution is injected into the positive pressure injection pump and the negative pressure injection pump of the electrospinning machine, the electrospinning machine is turned on, the spiral ridge collector is rotated by the rotating motor, the positive pressure spinning nozzle and the negative pressure spinning nozzle spin yarns from both sides towards the spiral ridge collector, and an electrospinning layer with a thickness of 100-160 μm is formed on the circumferential outside of the spiral ridge collector.

[0021] As an improvement to the above scheme, the voltage of the electrospinning machine is 10-15 kV, the distance between the positive pressure spinning nozzle and the negative pressure spinning nozzle and the spiral ridge collector is 15-20 cm, the rotating speed of the spiral ridge collector is 200-500 r / min, the spinning speed of the positive pressure spinning nozzle and the negative pressure spinning nozzle is 0.5-1 ml / h, the spinning temperature is 28-30 °C, and the spinning relative humidity is 45%-50%.

[0022] As an improvement to the above scheme, in step S4, after the spiral ridge collector is taken down from the electrospinning machine, the connection between the first end and the second end of the metal wire and the mandrel is disconnected, the mandrel is taken out, and then the metal wire is taken out one by one to obtain the spiral ridge artificial blood vessel.

[0023] Another technical scheme adopted by the application is to provide a spiral ridge artificial blood vessel prepared by the spiral ridge artificial blood vessel preparation method, wherein the spiral ridge artificial blood vessel comprises n spiral ridges, and n is an integer greater than or equal to 3.

[0024] Further, the rotation angle of the spiral ridge is 30°-60°, and the amplitude is 0.5-1 mm; the diameter of the spiral ridge artificial blood vessel is 4-9 mm, and the wall thickness is 100-160 μm.

[0025] Differently from the prior art, the spiral ridge artificial blood vessel preparation method provided by the application tightly surrounds multiple metal wires outside a cylindrical mandrel to make a spiral ridge collector, fixes the spiral ridge collector on an electrostatic spinning machine, and prepares a spiral ridge artificial blood vessel by an electrostatic spinning method. The spiral ridge artificial blood vessel prepared has multiple spiral ridges on the inner wall, can induce blood to generate rotational flow, can not only improve the near-wall blood flow velocity and wall shear stress, but also reduce the deposition of harmful substances on the inner wall of the blood vessel, has important significance for reducing the restenosis after small-diameter artificial blood vessel transplantation and improving long-term patency, and the application further provides a spiral ridge artificial blood vessel prepared by the spiral ridge artificial blood vessel preparation method. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 is a structure diagram of a spiral ridge collector and a cross section thereof in the first embodiment of the application;

[0027] Figure 2 is a structure diagram of a spiral ridge collector and a cross section thereof in the second embodiment of the application. DETAILED DESCRIPTION

[0028] The application provides a spiral ridge artificial blood vessel preparation method for preparing a spiral ridge artificial blood vessel with a diameter of D, wherein the spiral ridge artificial blood vessel has spiral ridges on the inner wall, which is beneficial to form blood rotational flow.

[0029] The spiral ridge artificial blood vessel preparation method comprises the following steps:

[0030] S1. Spiral ridge collector preparation:

[0031] (1) providing a cylindrical mandrel and n bendable cylindrical metal wires, wherein the diameter of the mandrel is D1, the diameter of the metal wires is D2, n is an integer greater than or equal to 3, and D1=D2 / sin(180° / n)-D2;

[0032] (2) making the n metal wires parallel to the mandrel and surrounding the mandrel in parallel, and connecting the first ends of the n metal wires to the mandrel;

[0033] (3) rotating the second ends of the n metal wires opposite to the first ends along the outer circumferential surface of the mandrel in a predetermined direction synchronously by a predetermined angle, so that the n metal wires are tightly wound on the outer circumferential surface of the mandrel in a spiral ridge shape;

[0034] (4) connecting the second ends of the n metal wires with the mandrel to obtain a spiral ridge collector;

[0035] S2. Electrospinning solution configuration:

[0036] An electrospinning solution with a concentration of 8% to 12% is prepared by taking poly-L-lactide-caprolactone (PLCL) or polyurethane (PU) as solute and hexafluoroisopropanol, trichloromethane or tetrahydrofuran as solvent.

[0037] S3. Spiral ridge artificial blood vessel forming:

[0038] The spiral ridge collector is installed on an electrospinning machine, the electrospinning solution is injected into the electrospinning machine for electrospinning, and an electrospinning layer with a predetermined thickness is formed on the circumferential outside of the spiral ridge collector.

[0039] S4. Demolding:

[0040] The spiral ridge collector is removed from the electrospinning machine, the electrospinning layer is separated from the spiral ridge collector, and a spiral ridge artificial blood vessel is obtained.

[0041] The spiral ridge artificial blood vessel preparation method tightly winds a plurality of metal wires around the outside of a cylindrical mandrel to make a spiral ridge collector, and prepares a spiral ridge artificial blood vessel by an electrospinning method. The spiral ridge artificial blood vessel has a plurality of spiral ridges on the inner wall, can induce blood to generate rotational flow, can not only improve the near-wall blood flow velocity and wall shear stress, but also reduce the deposition of harmful substances on the inner wall of the blood vessel, and has important significance for reducing the restenosis after small-diameter artificial blood vessel transplantation and improving long-term patency.

[0042] The electrospinning method is a common method for preparing nanofibers. By using different collectors, nanofibers can be collected into samples with different shapes, such as fiber membranes and tubular objects. At present, the collectors used for preparing tubular objects are all metal rods with smooth surfaces, and artificial blood vessels prepared by the electrospinning method are prepared by such collectors.

[0043] In the present application, a cylindrical metal rod with a smooth surface is used as a mandrel. A spiral ridge collector obtained by tightly winding a plurality of bendable cylindrical metal wires around the outside of the mandrel and rotating the metal wires is a non-smooth-surface collector, which is a technical breakthrough in the design of the collector for preparing tubular objects by the electrospinning method.

[0044] In a preferred embodiment, the mandrel is a cylindrical copper rod with a smooth surface.

[0045] In the present application, the helical ridge collector is prepared by selecting a predetermined metal rod diameter and a metal wire diameter, and it can be understood that the diameter of the helical ridge collector corresponds to the diameter D of the helical ridge artificial blood vessel prepared by the helical ridge collector.

[0046] Specifically, the diameter D1 of the mandrel, the diameter D2 of the metal wire and the number n of the metal wires can be adaptively adjusted according to the structural requirements such as the diameter of the helical ridge artificial blood vessel to be prepared, the number of helical ridges, the amplitude of the helical ridges, etc.

[0047] The diameter D1 of the mandrel, the diameter D2 of the metal wire and the number n of the metal wires satisfy the following conditions: n is an integer greater than or equal to 3, and D1 = D2 / sin(180° / n) - D2, so as to ensure that the n metal wires are parallel to the mandrel and can be closely and side-by-side wound on the outer peripheral surface of the mandrel, i.e. the distance between the adjacent two metal wires is 0.

[0048] Preferably, n is an even integer greater than or equal to 3.

[0049] It can be understood that when n is an even integer, the diameter of the helical ridge collector is equal to D1 + 2D2, and therefore the diameter D of the helical ridge artificial blood vessel to be prepared is equal to D1 + 2D2.

[0050] Among them, the amplitude of the helical ridge of the helical ridge artificial blood vessel to be prepared corresponds to the radius of the metal wire.

[0051] Further, in step S1, D2 ≤ D1.

[0052] Further, the diameter D of the helical ridge artificial blood vessel to be prepared is set to 4-9 mm, the diameter D1 of the mandrel is set to 2-5 mm, and the diameter D2 of the metal wire is set to 1-2 mm.

[0053] The length of the metal wire is not less than the length of the helical ridge artificial blood vessel, and preferably the length of the metal wire is equal to the length of the helical ridge artificial blood vessel.

[0054] In step S3, when the helical ridge collector is installed in the electrospinning machine, the two ends of the mandrel are connected with the rotating motor of the electrospinning machine, and therefore the length of the mandrel is set to be greater than the length of the metal wire so as to facilitate the connection of the two ends of the mandrel with the rotating motor, and specifically the length of the mandrel is set to be 15%-20% greater than the length of the metal wire.

[0055] Further, in step S1, the first end of the metal wire is fixedly connected with the mandrel by a buckle, so as to facilitate the rotation of the metal wire along the outer circumferential surface of the mandrel, and after the second end of the n metal wires is synchronously rotated by a predetermined angle along the outer circumferential surface of the mandrel in a predetermined direction, the second end of the metal wire is fixedly connected with the mandrel by a buckle, so that the n metal wires are tightly wound on the outer circumferential surface of the mandrel in the form of spiral ridges, i.e., the spiral ridge collector.

[0056] Since the metal wire is selected from a material that can be bent and shaped, after the second end of the n metal wires is synchronously rotated by a predetermined angle along the outer circumferential surface of the mandrel in a predetermined direction, the metal wire is rotated in the entire length direction, so that n spiral ridges tightly wound on the outer circumferential surface of the mandrel are formed.

[0057] In a preferred embodiment, the metal wire is a copper wire that can be bent and shaped.

[0058] Further, the predetermined direction is clockwise or counterclockwise, and the predetermined angle is set to 30°-60°, wherein the predetermined angle is the central angle corresponding to the circular arc of the mandrel rotated by the metal wire along the outer circumferential surface of the mandrel.

[0059] When the first end and the second end of the metal wire are fixedly connected with the mandrel, a predetermined distance is reserved between the first end and the second end of the metal wire and the corresponding end of the mandrel, so as to facilitate the installation of the spiral ridge collector and the rotating motor through the mandrel.

[0060] Preferably, the predetermined distance reserved between the first end, the second end and the corresponding end of the mandrel is equal.

[0061] Further, in step S2, a static spinning solution with a concentration of 10% is prepared by taking poly-L-lactide-caprolactone (PLCL) as a solute and hexafluoroisopropanol as a solvent.

[0062] In this step, the hexafluoroisopropanol solution of PLCL is used as the static spinning solution. It should be noted that the static spinning solution can also be selected from other solutes, such as polyurethane (PU) and other polymers, and the static spinning solution can also use trichloromethane, tetrahydrofuran and other organic solvents as solvents.

[0063] Further, in step S3, the static spinning solution is injected into the positive pressure injection pump and the negative pressure injection pump of the electrostatic spinning machine, the electrostatic spinning machine is turned on, the rotating motor drives the spiral ridge collector to rotate, and the positive pressure spinning needle and the negative pressure spinning needle spray spinning fibers from both sides to the spiral ridge collector, so as to form a static spinning layer with a thickness of 100-160 μm on the circumferential outside of the spiral ridge collector.

[0064] In this application, the static spinning layer constitutes the blood vessel wall of the spiral ridge artificial blood vessel to be prepared.

[0065] It can be understood that, in the actual preparation process, the spinning time of the positive pressure spinning needle and the negative pressure spinning needle can be controlled according to the thickness requirement of the blood vessel wall of the spiral ridge artificial blood vessel to be prepared. The longer the spinning time is, the thicker the electrospinning layer is, and the thicker the blood vessel wall of the prepared spiral ridge artificial blood vessel is.

[0066] The electrospinning machine comprises two high-voltage direct current power supplies, namely a positive pressure direct current power supply and a negative pressure direct current power supply, the positive pressure direct current power supply is used for outputting positive pressure, the negative pressure direct current power supply is used for outputting negative pressure, the positive pressure spinning needle is connected to the positive pressure direct current power supply, and the negative pressure spinning needle is connected to the negative pressure direct current power supply.

[0067] The voltage of the positive pressure direct current power supply is set to 5-6 kV, and the voltage of the negative pressure direct current power supply is set to -6--5 kV.

[0068] The spiral ridge collector is arranged between the positive pressure spinning needle and the negative pressure spinning needle, the positive pressure spinning needle and the negative pressure spinning needle are oppositely arranged and located at opposite sides above the spiral ridge collector, and the positive pressure spinning needle and the negative pressure spinning needle spin from the two sides to the spiral ridge collector in a slanting downward direction to form conjugate electrospinning.

[0069] Further, the voltage of the electrospinning machine is 10-15 kV, the distance between the positive pressure spinning needle, the negative pressure spinning needle and the spiral ridge collector is 15-20 cm, the rotating speed of the spiral ridge collector is 200-500 r / min, the spinning speed of the positive pressure spinning needle and the negative pressure spinning needle is 0.5-1 ml / h, the spinning temperature is set to 28-30℃, and the spinning relative humidity is set to 45%-50%.

[0070] Further, in step S4, after the spiral ridge collector is taken out of the electrospinning machine, the connection between the first end and the second end of the metal wire and the mandrel is disconnected, the mandrel is taken out, and then the metal wire is taken out one by one to obtain the spiral ridge artificial blood vessel.

[0071] The spiral ridge collector can successfully separate the spiral ridge artificial blood vessel and the spiral ridge collector without damaging the mechanical properties of the artificial blood vessel and the shape of the spiral ridge, and the demolding is convenient.

[0072] Further, after the spiral ridge collector is taken out of the electrospinning machine and placed in a fume hood for drying for 24 h to remove residual organic solvents, the connection between the first end and the second end of the metal wire and the mandrel is disconnected, the mandrel is taken out, and then the metal wire is taken out to obtain the spiral ridge artificial blood vessel.

[0073] In one application scenario, the preparation method of the spiral ridge artificial blood vessel further comprises the steps of drying and storing the spiral ridge artificial blood vessel obtained in step S4. Specifically, the spiral ridge artificial blood vessel obtained in step S4 is naturally dried and stored in a self-sealing bag.

[0074] The application will be further described in detail below with reference to examples. It is particularly pointed out that the following examples are only used to illustrate the application, but do not limit the scope of the application. Similarly, the following examples are only part of the examples of the application, not all examples, and all other examples obtained by those skilled in the art without creative labor are within the scope of the application.

[0075] Example 1

[0076] The present embodiment provides a preparation method of a spiral ridge artificial blood vessel, which is intended to prepare a spiral ridge artificial blood vessel with a length of 40 mm and a diameter D of 6 mm. The preparation method comprises the following steps:

[0077] S1. Spiral ridge collector preparation:

[0078] (1) Provide a cylindrical metal rod with a smooth surface as a mandrel and 6 bendable cylindrical metal wires, wherein the mandrel has a length of 48 mm and a mandrel diameter D1 = 2 mm, and the metal wires have a length of 40 mm and a wire diameter D2 = 2 mm;

[0079] (2) Wrap the 6 metal wires tightly side by side around the outer peripheral surface of the mandrel parallel to the mandrel, and connect the first ends of the 6 metal wires to the mandrel by buckling;

[0080] Leave a distance of 4 mm between the first end of the metal wire and the corresponding end of the mandrel;

[0081] (3) Rotate the second ends of the 6 metal wires opposite to the first ends along the outer peripheral surface of the mandrel in a counterclockwise direction by 60° synchronously, so that the 6 metal wires are tightly wrapped around the outer peripheral surface of the mandrel in the form of spiral ridges;

[0082] (4) Connect the second ends of the 6 metal wires to the mandrel by buckling to obtain a spiral ridge collector.

[0083] Leave a distance of 4 mm between the second end of the metal wire and the corresponding end of the mandrel to facilitate the installation of the spiral ridge collector and the rotary motor through the mandrel.

[0084] Please refer to Figure 1 , Figure 1 The structure of the spiral ridge collector and its cross section in the present embodiment is shown.

[0085] S2. Electrospinning liquid configuration:

[0086] 1 g of poly-L-lactide-caprolactone (PLCL) was weighed out to prepare a PLCL solution with a concentration of 10% as an electrospinning solution, and hexafluoroisopropanol was used as a solvent.

[0087] S3. Spiral ridge artificial blood vessel forming:

[0088] The spiral ridge collector was installed on the electrospinning machine, and the electrospinning solution was injected into the electrospinning machine for electrospinning for 2 h to form an electrospinning layer on the outer circumference of the spiral ridge collector.

[0089] In this embodiment, the thickness of the electrospinning layer was 100 μm.

[0090] In this embodiment, the voltage of the positive voltage direct current power supply was set to +5.8 kV, the voltage of the negative voltage direct current power supply was set to -5.2 kV, the voltage of the electrospinning machine was 11 kV, the distance between the positive voltage spinning needle and the negative voltage spinning needle and the spiral ridge collector was 16 cm, the distance between the two spinning needles was 28 cm, the rotation speed of the spiral ridge collector was 300 r / min, and the spinning speed of the positive voltage spinning needle and the negative voltage spinning needle was 0.5 mL / h; the spinning temperature was 29.8 °C, and the relative humidity was 48%.

[0091] S4. Demolding:

[0092] The spiral ridge collector was removed from the electrospinning machine and placed in a fume hood for drying for 24 h to remove residual organic solvents, the buckle connecting the metal wires and the mandrel was removed, the mandrel was removed, and then the metal wires were removed one by one to obtain the spiral ridge artificial blood vessel.

[0093] The spiral ridge artificial blood vessel had a length of 40 mm, a diameter of 6 mm, a wall thickness of 100 μm, and included 6 spiral ridges, the amplitude of the spiral ridges was 1 mm, and the rotation angle of the spiral ridges was 60°.

[0094] It can be understood that the rotation angle of the spiral ridges corresponds to the angle of the second ends of the 6 metal wires rotating synchronously in the counterclockwise direction along the outer circumferential surface of the mandrel in this embodiment.

[0095] Example Two:

[0096] The present embodiment provides a preparation method of a spiral ridge artificial blood vessel, which is intended to prepare a spiral ridge artificial blood vessel with a length of 40 mm and a diameter D of 7.8 mm, and the preparation method comprises the following steps:

[0097] S1. Spiral ridge collector preparation:

[0098] (1) providing a cylindrical metal rod with smooth surface as a mandrel and 10 cylindrical metal wires which can be bent and shaped, wherein the mandrel has a length of 48 mm, a mandrel diameter D1 = 4.2 mm, the metal wires have a length of 40 mm, and a wire diameter D2 = 1.8 mm;

[0099] (2) making the 10 metal wires tightly and closely surround the outer circumferential surface of the mandrel in parallel to the mandrel, and connecting the first ends of the 10 metal wires to the mandrel by buckling;

[0100] Leaving a distance of 4 mm between the first ends of the metal wires and the corresponding end portions of the mandrel.

[0101] (3) rotating the second ends of the 10 metal wires which are opposite to the first ends along the outer circumferential surface of the mandrel in a counterclockwise direction by 36° synchronously, so that the 10 metal wires tightly surround the outer circumferential surface of the mandrel in a spiral ridge shape;

[0102] (4) connecting the second ends of the 10 metal wires to the mandrel by buckling to obtain a spiral ridge collector.

[0103] Leaving a distance of 4 mm between the second ends of the metal wires and the corresponding end portions of the mandrel, so as to facilitate the installation of the spiral ridge collector and the rotary motor through the mandrel.

[0104] Please refer to Figure 1 , Figure 1 The structure of the spiral ridge collector and its cross section in the embodiment are shown.

[0105] S2. Electrospinning solution configuration:

[0106] Taking 1 g of poly-L-lactide-caprolactone (PLCL), a PLCL solution with a concentration of 10% is prepared as an electrospinning solution, and hexafluoroisopropanol is used as a solvent.

[0107] S3. Spiral ridge artificial blood vessel forming:

[0108] Installing the spiral ridge collector on an electrospinning machine, injecting the electrospinning solution into the electrospinning machine for electrospinning for 3 h, and forming an electrospinning layer on the circumferential outside of the spiral ridge collector.

[0109] In the embodiment, the thickness of the electrospinning layer is 150 μm.

[0110] In the embodiment, the voltage of the positive voltage DC power supply is set to +5.5 kV, the voltage of the negative voltage DC power supply is set to -5.5 kV, the voltage of the electrospinning machine is 11 kV, the distance between the positive voltage spinning needle, the negative voltage spinning needle and the spiral ridge collector is 16 cm, the distance between the two spinning needles is 28 cm, the rotating speed of the spiral ridge collector is 300 r / min, the spinning speed of the positive voltage spinning needle and the negative voltage spinning needle is 0.5 mL / h; the spinning temperature is 29 ℃, and the relative humidity is 50%.

[0111] S4. Demolding:

[0112] The spiral ridge collector is taken off from the electrospinning machine and placed in a fume hood for drying for 24 h to remove residual organic solvents, the buckle connecting the metal wires and the mandrel is taken off, the mandrel is taken out, and then the metal wires are taken out one by one to obtain the spiral ridge artificial blood vessel.

[0113] The spiral ridge artificial blood vessel has a length of 40 mm, a diameter of 7.8 mm, a wall thickness of 150 μm, and includes 10 spiral ridges, the amplitude of the spiral ridges is 0.9 mm, and the rotating angle of the spiral ridges is 36°.

[0114] It can be understood that the rotating angle of the spiral ridges corresponds to the angle of the second ends of the 10 metal wires rotating synchronously in the anticlockwise direction along the outer circumferential surface of the mandrel in the embodiment.

[0115] The application further provides a spiral ridge artificial blood vessel prepared by the above-mentioned method for preparing a spiral ridge artificial blood vessel, and the spiral ridge artificial blood vessel includes n spiral ridges, and n is an integer greater than or equal to 3.

[0116] Further, the rotating angle of the spiral ridges is 30°-60°, and the amplitude is 0.5-1 mm; the diameter of the spiral ridge artificial blood vessel is 4-9 mm, and the wall thickness is 100-160 μm.

[0117] The spiral ridge artificial blood vessel has a plurality of spiral ridges on the inner wall, can induce the blood to generate rotational flow, can not only improve the near-wall blood flow velocity and wall shear stress, but also can reduce the deposition of harmful substances on the inner wall of the blood vessel, and has important significance for reducing the restenosis after small-diameter artificial blood vessel transplantation and improving long-term patency.

[0118] The above only describes the embodiments of the application, and does not limit the patent scope of the application, and any equivalent structure or equivalent process transformation using the content of the specification and drawings, or direct or indirect application in other related technical fields, are also included in the patent protection scope of the application.

Claims

1. A method for preparing a helical-ridge artificial blood vessel for preparing a helical-ridge artificial blood vessel having a diameter D, characterized by, The method comprises the following steps: S1. Spiral ridge collector preparation: (1) providing a cylindrical mandrel and n bendable cylindrical metal wires, the diameter of the mandrel is D1, the diameter of the metal wire is D2, wherein n is an integer greater than or equal to 3, and D1=D2 / sin(180° / n)-D2; The diameter D2 of the metal wire is 1-2 mm, and the spiral ridge amplitude of the spiral ridge artificial blood vessel corresponds to the radius of the metal wire, and D1 is set to 2-5 mm; The first end and the second end of the metal wire are fixedly connected to the mandrel by buckling, and a predetermined distance is provided between the first end and the second end of the metal wire and the corresponding end of the mandrel; (2) arranging the n metal wires in parallel to the mandrel and in parallel around the outer circumferential surface of the mandrel, and connecting the first end of the n metal wires to the mandrel; (3) rotating the second end of the n metal wires opposite to the first end along the outer circumferential surface of the mandrel in a predetermined direction by a predetermined angle synchronously, so that the n metal wires are tightly wound around the outer circumferential surface of the mandrel in the form of spiral ridges; (4) connecting the second end of the n metal wires to the mandrel to obtain a spiral ridge collector; S2. Electrospinning solution configuration: An electrospinning solution with a concentration of 8%-12% is prepared by taking poly-L-lactide-caprolactone (PLCL) or polyurethane (PU) as a solute and taking hexafluoroisopropanol, trichloromethane or tetrahydrofuran as a solvent; S3. Spiral ridge artificial blood vessel forming: The spiral ridge collector is installed on an electrospinning machine, the electrospinning solution is injected into the electrospinning machine for electrospinning, and an electrospinning layer with a predetermined thickness is formed on the circumferential outside of the spiral ridge collector; S4. Demolding: The spiral ridge collector is taken off from the electrospinning machine, the connection between the first end and the second end of the metal wire and the mandrel is disconnected, the mandrel is taken out, and then the metal wires are taken out one by one to obtain the spiral ridge artificial blood vessel.

2. The method of claim 1, wherein the helical-ridge vascular graft is prepared by the steps of: In step S1, the predetermined direction is clockwise or counterclockwise, and the predetermined angle is 30°-60°.

3. The method of claim 1, wherein the helical-ridge vascular graft is prepared by the steps of: D is set to 4-9 mm.

4. The method of claim 1, wherein the helical-ridge vascular graft is prepared by the steps of: The length of the metal wire is not less than the length of the spiral ridge artificial blood vessel, and the length of the mandrel is 15%-20% greater than the length of the metal wire.

5. The method of claim 1, wherein the helical-ridge vascular graft is prepared by the steps of: In step S3, the electrospinning solution is injected into a positive pressure injection pump and a negative pressure injection pump of the electrospinning machine, the electrospinning machine is started, the spiral ridge collector is rotated by a rotating motor, and positive pressure spinning nozzles and negative pressure spinning nozzles spray spinning threads from both sides to the spiral ridge collector to form an electrospinning layer with a thickness of 100-160 μm on the circumferential outside of the spiral ridge collector.

6. The method of claim 5, wherein the helical-ridge vascular graft is prepared by the steps of: The voltage of the electrospinning machine is 10-15 kV, the distance between the positive pressure spinning nozzles and the negative pressure spinning nozzles and the spiral ridge collector is 15-20 cm, the rotating speed of the spiral ridge collector is 200-500 r / min, the spinning speed of the positive pressure spinning nozzles and the negative pressure spinning nozzles is 0.5-1 ml / h, the spinning temperature is 28-30 ℃, and the spinning relative humidity is 45%-50%.

7. A helical-ridge vascular graft, characterized by, The spiral ridge artificial blood vessel is prepared by the method of claim 1, and the spiral ridge artificial blood vessel comprises n spiral ridges, wherein n is an integer greater than or equal to 3.

8. The helical ridge vascular graft of claim 7, wherein, The rotation angle of the spiral ridge is 30-60°, and the amplitude is 0.5-1mm; the diameter of the spiral ridge artificial blood vessel is 4-9mm, and the wall thickness is 100-160μm.

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