A Microneedle Mold Fixing Device Based on Centrifugal Operation and a Microneedle Manufacturing Method

Abstract

The present invention discloses a microneedle mold fixing device and a microneedle manufacturing method based on centrifugal operation, wherein the microneedle mold fixing device comprises: a centrifuge tube, wherein the centrifuge tube has an inner cavity; a padding portion, wherein the padding portion can be detachably arranged in the inner cavity and is used to carry the microneedle mold; a sealing portion, wherein the sealing portion can be detachably arranged in the inner cavity and covers the opening of the microneedle mold; a pulling portion, wherein the pulling portion is connected to the surface of the sealing portion away from the padding portion; and the outer side wall of the padding portion and the outer side wall of the sealing portion both abut against the inner wall of the inner cavity. The problem that the kit in the prior art cannot be universally used and is fixed by glue, adhesive paper and mechanical reinforcement methods, resulting in inconvenient placement, unstable structure, easy imbalance and liquid overflow, is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of microneedle manufacturing, and in particular to a microneedle mold fixing device based on centrifugal operation and a microneedle manufacturing method. Background Art

[0002] The colloidal material is poured into the micro mold and the colloid is made to penetrate into the groove of the master mold by centrifugation. The existing method usually fixes the mold directly in a glue spreader or a centrifuge. When the glue spreader is used for spin coating, the colloid is difficult to penetrate vertically, while the centrifuge can achieve the effect of vertical penetration. In this method, it is necessary to customize special fixings to stabilize the mold to maintain balance, which is time-consuming and labor-intensive.

[0003] When using a microneedle mold for centrifugal operation, how to fix the microneedle mold is a difficult problem. Usually, people fix it by customizing a kit that matches the microneedle mold, but due to the different specifications of the microneedle mold, and usually a large number of microneedle molds (many specifications), the kit cannot be universal, resulting in customized kits of different specifications are often expensive, and there are few manufacturers; or fix it by methods such as glue, sticky paper and mechanical reinforcement, which has the disadvantages of inconvenient placement, unstable structure, easy imbalance and liquid overflow.

[0004] Therefore, the prior art still needs to be improved and developed. Summary of the invention

[0005] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a microneedle mold fixing device and a microneedle manufacturing method based on centrifugal operation, so as to solve the problems in the prior art that the kit is not universal, and the fixing by glue, sticky paper and mechanical reinforcement methods causes inconvenient taking and placing, unstable structure, easy imbalance and liquid overflow.

[0006] The technical solution of the present invention is as follows:

[0007] A microneedle mold fixing device based on centrifugal operation includes: a centrifuge tube having an inner cavity;

[0008] A padding portion, which can be detachably arranged in the inner cavity and is used to carry the microneedle mold;

[0009] A sealing part, which is detachably located in the inner cavity and covers the opening of the microneedle mold;

[0010] A pulling portion connected to a surface of the sealing portion facing away from the raised portion;

[0011] The outer side wall of the raised portion and the outer side wall of the sealing portion both abut against the inner wall of the inner cavity.

[0012] Optionally, the pulling portion includes: a screw, the screw being screwed on the sealing portion;

[0013] A pull rope, the pull rope being connected to the screw.

[0014] Optionally, the heightening part is a plastic heightening part, and the upper surface of the plastic heightening part abuts against the lower surface of the microneedle mold.

[0015] Optionally, the lower surface of the sealing part fits with the opening surface of the microneedle mold.

[0016] Optionally, the sealing part is made of an elastomeric material.

[0017] Optionally, the sealing part is formed by uniformly mixing an adhesive and a curing agent and curing.

[0018] Optionally, the microneedle mold fixing device further includes a cover body, and the cover body is screwed onto the centrifuge tube and seals the inner cavity.

[0019] Optionally, the height of the heightening part + the height of the microneedle mold + the height of the sealing part is less than or equal to the height of the centrifuge tube.

[0020] Based on the same concept, the present invention also provides a microneedle manufacturing method, which is used for the microneedle mold fixing device described in any one of the above, and the microneedle manufacturing method includes the steps of:

[0021] Prepare a microneedle precursor solution;

[0022] Take an appropriate amount of the solution and inject it into the microneedle mold;

[0023] Put it into the microneedle mold fixing device for centrifugation;

[0024] Dry;

[0025] Take out the microneedle mold from the microneedle mold fixing device, and take out the formed microneedles from the microneedle mold.

[0026] Optionally, in the step of putting it into the microneedle mold fixing device for centrifugation:

[0027] The centrifugation speed is 3000 rpm, and the centrifugation time is 5 minutes;

[0028] In the step of drying, the drying temperature is: 30 °C, and the standing time is 8 hours.

[0029] Beneficial effect: Compared with the prior art, the present invention proposes a microneedle mold fixing device and a microneedle manufacturing method based on centrifugal operation, wherein the microneedle mold fixing device is placed in a centrifuge through a centrifuge tube, and is detachably arranged in the inner cavity of the centrifuge tube through a cushioning part, so that the cushioning part can be easily replaced to match different microneedle molds, and the microneedle mold is placed on the cushioning part, and the cushioning part raises the microneedle mold to facilitate the storage and retrieval of the microneedle mold; when in use, the prepared liquid, colloid and other impression materials are added to the opening of the microneedle mold, and the sealing part is detachably covered with the opening of the microneedle mold, thereby sealing the inner cavity of the microneedle mold, so that the impression material is sealed in the inner cavity of the microneedle mold and is molded through the inner cavity of the microneedle mold; and the sealing part is detachably located in the inner cavity, which is convenient for the sealing part to be taken out of the inner cavity, or the sealing part is replaced according to different microneedle molds. The outer wall of the raised part and the outer wall of the pulling part are both against the inner wall of the inner cavity, so that the raised part and the sealing part will not move in the radial direction of the centrifuge tube, and the raised part and the sealing part fix the microneedle mold in the axial direction, so that the microneedle mold will not shake and cause imbalance during the centrifugation process. In this scheme, as long as the microneedle mold is smaller than the inner cavity of the centrifuge tube, it can be fixed, so microneedle molds of different specifications can be fixed between the raised part and the sealing part, which has universality. The size and height of the raised part and the sealing part can be made according to the size of the microneedle mold and the size of the centrifuge tube. The material is convenient to obtain, the process is simple, the matching degree with the microneedle mold and the centrifuge tube is high, the fixation is stable so that the colloid is not easy to overflow, and the microneedle mold will not shake and cause imbalance during the centrifugation process; in addition, the microneedle mold fixing device has a simple structure, low cost, and the raised part and the sealing part are easy to remove and separate from the centrifuge tube, which is convenient to clean and can be used repeatedly, thus avoiding the problems of inconvenient placement, unstable structure, easy imbalance and liquid overflow in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a cross-sectional view of a first embodiment of a microneedle mold fixing device based on centrifugal operation of the present invention;

[0031] Figure 2 A top view of a microneedle mold of an embodiment of a microneedle mold fixing device based on centrifugal operation of the present invention;

[0032] Figure 3 This is a front view of the existing microneedle;

[0033] Figure 4 It is a cross-sectional view of a second embodiment of a microneedle mold fixing device based on centrifugal operation of the present invention;

[0034] Figure 5 This is a cross-sectional view of Example 3 of a microneedle mold fixing device based on centrifugal operation of the present invention.

[0035] Reference numerals in the figure: 100, centrifuge tube; 200, elevation part; 300, sealing part; 310, counterbore; 400, pulling part; 410, screw; 420, pulling rope; 500, microneedle mold; 510, concave cavity; 600, cover body; 700, push rod; 900, height adjustment assembly; 910, adjusting screw; 920, bearing block; 921, internal thread hole; 922, guide groove; 930, guide block. Detailed implementation manners

[0036] The present invention provides a microneedle mold fixing device and a microneedle manufacturing method based on centrifugal operation. To make the objectives, technical solutions and effects of the present invention clearer and more definite, the following further describes the present invention in detail with reference to the accompanying drawings and by way of examples. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0037] Microneedles can carry a variety of drugs, such as weight loss drugs, anti-skin cancer drugs, drug-loaded nanoparticles, etc. It is a new type of drug delivery method. Due to characteristics such as short length, small volume, ability to penetrate the stratum corneum, and safe and healthy manufacturing materials, it can overcome problems such as low drug utilization rate and slow treatment in traditional drug-taking treatments, pain and damage to normal human tissues in injection treatments, as well as possible cross-infections and environmental pollution caused by injection tools. When manufacturing microneedles, a colloidal material is poured into the microneedle mold, and the drug-loaded colloid is infiltrated into the grooves of the microneedle mold (master mold) by centrifugation. Usually, the microneedle mold is fixed in a spin coater or a centrifuge, a colloidal material loaded with drugs is added to the microneedle mold, and after spin coating / centrifugation, it is dried and demolded to obtain the required microneedle device.

[0038] This solution proposes multiple embodiments, which are specifically as follows:

[0039] Embodiment 1

[0040] As Figure 1 shown, this embodiment proposes a microneedle mold fixing device based on centrifugal operation, which is used to fix the microneedle mold 500 on a centrifugal device. As Figure 2 shown, the specific structure of the microneedle mold 500 includes a master mold. One side of the master mold is an opening, and inside the opening is a concave cavity 510 with a recessed pattern on the inner wall. By adding prepared liquid, colloid and other impression materials to the opening of the microneedle mold 500, microneedles are formed after curing. The structure of the microneedles is as Figure 3As shown, its structure is prior art and will not be described in detail. The micro-needle mold 500 fixing device includes: a centrifuge tube 100, a padding part 200, a sealing part 300, and a pulling part 400. The centrifuge tube 100 has an inner cavity. The padding part 200 is detachably arranged in the inner cavity and is used to carry the micro-needle mold 500. The padding part 200 can be directly placed into the inner cavity of the centrifuge tube 100, and the micro-needle mold 500 is placed on the padding part 200. The sealing part 300 is detachably located in the inner cavity and covers the opening of the micro-needle mold 500, thereby sealing the formulated liquid, colloid and other impression materials in the micro-needle mold 500 in the opening of the micro-needle mold 500. The pulling part 400 is connected to the surface of the sealing part 300 facing away from the padding part 200. After the micro-needles are formed, the sealing part 300 can be directly pulled out of the inner cavity of the centrifuge tube 100 through the pulling part 400, facilitating the disassembly of the sealing part 300. The outer side walls of the padding part 200 and the sealing part 300 both abut against the inner wall of the inner cavity, and the outer dimension of the micro-needle mold 500 is smaller than the inner dimension of the centrifuge tube 100, so that different-sized micro-needle molds 500 can be accommodated in the centrifuge tube 100.

[0041] The working principle of a micro-needle mold fixing device based on centrifugal operation provided in this embodiment is as follows: The micro-needle mold 500 fixing device is placed in a centrifuge through the centrifuge tube 100, and the padding part 200 is detachably arranged in the inner cavity of the centrifuge tube 100, enabling the padding part 200 to be conveniently replaced to match different micro-needle molds 500. The micro-needle mold 500 is placed on the padding part 200, and the padding part 200 raises the micro-needle mold 500, facilitating the access to the micro-needle mold 500; during use, the formulated liquid, colloid and other impression materials are added to the opening of the micro-needle mold 500, and the sealing part 300 detachably covers the opening of the micro-needle mold 500, thereby closing the concave cavity 510 of the micro-needle mold 500, sealing the impression materials in the concave cavity 510 of the micro-needle mold 500 and forming through the concave cavity 510 of the micro-needle mold 500; and the sealing part 300 is detachably located in the inner cavity, facilitating the removal of the sealing part 300 from the inner cavity or the replacement of the sealing part 300 according to different micro-needle molds 500. The outer side walls of the padding part 200 and the pulling part 400 both abut against the inner wall of the inner cavity, so that the padding part 200 and the sealing part 300 will not move radially in the centrifuge tube 100, and the padding part and the sealing part 300 axially fix the micro-needle mold 500, so that the micro-needle mold 500 will not shake and cause imbalance during the centrifugation process.

[0042] The beneficial effects of the micro-needle mold fixing device based on centrifugal operation provided in this embodiment are at least as follows: In this solution, any micro-needle mold 500 smaller than the inner cavity of the centrifuge tube 100 can be fixed. Therefore, different specifications of micro-needle molds 500 can be fixed between the elevation part 200 and the sealing part 300, which has universality. The size and height of the elevation part 200 and the sealing part 300 can be made according to the size of the micro-needle mold 500 and the size of the centrifuge tube 100. The materials are convenient to obtain, the process is simple, the matching degree with the micro-needle mold 500 and the centrifuge tube 100 is high, the fixation is stable and the colloid is not easy to overflow, and the micro-needle mold 500 will not shake during centrifugation to cause imbalance; in addition, the structure of this micro-needle mold 500 fixing device is simple, the cost is low, the elevation part 200 and the sealing part 300 are easy to be removed from the centrifuge tube 100 for separation, which is convenient for cleaning and can be reused. Therefore, the problems of inconvenient picking and placing, unstable structure, easy imbalance and liquid overflow in the prior art are avoided.

[0043] It should be noted that for the solution of fixing the micro-needle mold 500 on the centrifugal equipment by the micro-needle mold fixing device based on centrifugal operation proposed in this solution, the centrifuge tube 100 can adopt various forms, the elevation part 200 can adopt various forms, the sealing part 300 can adopt various forms, and the pulling part 400 adopts various structures. However, no matter which form is adopted, as long as the micro-needle mold 500 is clamped in the centrifuge tube 100 by the elevation part 200 and the sealing part 300, and the outer side walls of the elevation part 200 and the sealing part 300 both abut against the inner wall of the inner cavity, so as to realize the fixation of the micro-needle mold 500, the problems in the prior art can be solved and corresponding effects can be obtained.

[0044] As Figure 1 shown, the centrifuge tube 100 in this embodiment can adopt a conical-bottom centrifuge tube or a round-bottom centrifuge tube. Among them, the conical-bottom centrifuge tube is generally for samples with a small sample volume and is easier to separate; the round-bottom centrifuge tube generally has a larger bottom area and is for samples with a large sample volume. In this embodiment, a conical bottom is selected to save materials. If a round bottom is adopted, a higher elevation part 200 is required, resulting in an increase in materials. Taking the conical-bottom centrifuge tube as an example for structural description, the bottom of the conical-bottom centrifuge tube is conical, the axial direction of the conical-bottom centrifuge tube is the axial direction in the structural description, and the axial direction is also the height direction; the radial direction of the conical-bottom centrifuge tube is the radial direction in the structural description.

[0045] As Figure 1As shown in the figure, the elevation part 200 can be first placed inside the centrifuge tube 100. The elevation part 200 is specifically a plastic elevation part 200. The upper surface of the plastic elevation part 200 abuts against the lower surface of the microneedle mold 500. The upper surface of the elevation part 200 has a certain plasticity or elasticity. For the microneedle mold 500 with an uneven lower surface, the surface of the elevation part 200 can undergo surface elastic deformation to fit with the lower surface of the micro mold, so that the microneedle mold 500 can be stably fixed on the elevation part 200, greatly reducing or avoiding the shaking of the microneedle mold 500. Another purpose of setting the elevation part 200 is to raise the microneedle mold 500, making the microneedle mold 500 closer to the mouth of the centrifuge tube 100, thus facilitating the picking and placing. The material of the elevation part 200 can be selected from elastic materials such as round wood, plasticine, rubber, etc. For example, if the elevation part 200 is cylindrical, the diameter of the elevation part 200 should match the inner diameter of the centrifuge tube 100, that is, the outer wall of the elevation part 200 is extrusion-fitted with the inner wall of the centrifuge tube 100, so as to avoid shaking and imbalance during the centrifugation process due to too small a size.

[0046] In another structure, in order to fix the microneedle mold 500 at a preset position (such as the middle position) on the elevation part 200, a special-shaped groove is provided at the preset position, and a special-shaped boss (not shown in the figure) matching the special-shaped groove is provided at the bottom of the microneedle mold 500. By matching the special-shaped groove and the special-shaped boss, the position of the microneedle mold 500 fixed on the elevation part 200 can be limited. Further, the microneedle mold 500 is kept stable during centrifugal rotation.

[0047] As Figure 1 , Figure 2 shown in the figure, the microneedle mold 500 is placed on the elevation part 200 and is carried by the elevation part 200, and the sealing part 300 covers the microneedle mold 500. The lower surface of the sealing part 300 fits with the opening surface of the microneedle mold 500. By making the lower surface of the sealing part 300 flat, the flat lower surface fits with the opening surface of the microneedle mold 500, thereby sealing the cavity 510 of the master mold, preventing the overflow of impression materials such as liquid and colloid during centrifugation, and enabling the impression material to fully fill the cavity 510 of the microneedle mold 500. The sealing part 300 can be cylindrical, and the diameter of the cylindrical sealing part 300 matches the inner diameter of the centrifuge tube 100, that is, the outer wall of the sealing part 300 is extrusion-fitted with the inner wall of the centrifuge tube 100. In this way, during centrifugation, the sealing part 300 does not shake, and together with the elevation part 200, the microneedle mold 500 is clamped in the inner cavity of the centrifuge tube 100.

[0048] The sealing part 300 in this embodiment is made of an elastic material. Elastic materials such as: impression material, PDMS, rubber, round wood, etc. Its production cost is low and it is easy to manufacture.

[0049] As Figure 1 , Figure 2 shown, the sealing part 300 in this embodiment is specifically formed by uniformly mixing an adhesive and a curing agent and then curing. The elastomeric impression material is divided into an adhesive and a curing agent. The manufacturing steps of the sealing part 300 are as follows: Take equal amounts of the adhesive and the curing agent, and both are colloidal fluids before mixing. Quickly mix the adhesive and the curing agent evenly, and mold them into a designed pattern (such as a cylindrical shape) and shape and size before curing; Let the shaped elastomeric impression material after mixing stand for about 30 minutes for curing; Check the cured elastomeric impression material to see if it matches the centrifuge tube 100. If it cannot be inserted into the centrifuge tube 100, perform manual modification to make the size of the sealing part 300 match the centrifuge tube 100. The cured elastic sealing part 300 in this embodiment is a cylinder with a diameter of about 2.5 cm and a height of about 3.5 cm.

[0050] As Figure 1 shown, the pulling part 400 in this embodiment includes: a screw 410 and a pull cord 420. The screw 410 is screwed onto the sealing part 300, and the pull cord 420 is connected to the screw 410. The pull cord 420 uses a thin string. The thin string is wound around the screw 410 and then fixed to the sealing part 300. By pulling the thin string, it is convenient to take out the sealing part 300.

[0051] In another structure, the pulling part 400 can also be a pull ring fixedly connected to the sealing part 300. During centrifugation, the pull ring is received below the upper surface of the sealing part 300. When it needs to be disassembled and cleaned, the pull ring is then rotated out, and the sealing part 300 is pulled out of the centrifuge tube 100 through the pull ring.

[0052] As Figure 1 shown, in another structure, a counterbore 310 is opened on the surface of the sealing part 300, and the screw 410 is placed in the counterbore 310 at the position, so that the head of the screw 410 can be located below the upper surface of the sealing part 300 to hide the screw 410, and the pull cord 420 can also be wound in the counterbore 310.

[0053] The micro-needle mold 500 fixing device in this embodiment further includes a cover body 600. The cover body 600 is screwed onto the centrifuge tube 100 and seals the inner cavity. The heightening part 200 and the sealing part 300 can be directly placed into the centrifuge tube 100 manually. The cover body 600 is manually tightened at the opening of the centrifuge tube 100 to seal the centrifuge tube 100.

[0054] The height of the elevation part 200 + the height of the microneedle mold 500 + the height of the sealing part 300 in this embodiment is less than or equal to the height of the centrifuge tube 100. In this way, the elevation part 200, the microneedle mold 500, and the sealing part 300 are all located inside the centrifuge tube 100, so that during centrifugation, the elevation part 200 and the sealing part 300 are stably fixed by the outer wall of the centrifuge tube 100.

[0055] In this embodiment, a 50 ml centrifuge tube 100 is selected, and a round log with a diameter of 2.5 cm and a height of 4 cm is used as the elevation part 200. A cylindrical PDMS with a diameter of 2.4 cm and a height of 0.8 cm is used as the sealing part 300. The microneedle mold 500 has 121 needles in an 11*11 array. Among them, the 50 ml centrifuge tube 100 has an inner diameter of 27 mm, an outer diameter of 30 mm, a pointed bottom height of 118 mm, and a round bottom height of 114 mm; the height of the elevation part 200 + the height of the microneedle mold 500 + the height of the sealing part 300 is less than the height of the centrifuge tube 100, but generally it is desired that the elevation part 200 is slightly higher for convenient mold placement and removal. For example, the height of the elevation part 200 can be 2 - 6 cm, the height of the microneedle mold 500 is 0.4 - 1.5 cm, and the height of the sealing part 300 is 2 - 6 cm; the microneedle mold 500 needs to be customized, and the number of needles can be variable and can be any number. Commonly used ones include 5*5, 7*7, 9*9, 10*10, 11*11, 20*20, etc.

[0056] Embodiment Two

[0057] As Figure 4 shown, based on the same concept, on the basis of Embodiment One, the centrifuge tube 100 is improved, and the elevation part 200 can be more conveniently taken out of the centrifuge tube 100.

[0058] The structure of this embodiment is: a hole is opened at the bottom of the centrifuge tube 100, and a push rod 700 can be arranged in the hole. Since the elevation part 200 is extruded inside the centrifuge tube 100, when the setting position of the elevation part 200 is relatively deep, it can be pushed by the push rod 700, so as to conveniently push the elevation part 200 towards the opening of the centrifuge tube 100, or loosen the elevation platform by the push rod 700.

[0059] In another solution, the hole is a threaded hole, and the push rod 700 is correspondingly set as a threaded push rod 700. The threaded push rod 700 is screwed into the threaded hole, and the elevation part 200 is pushed by screwing the threaded push rod 700, so that the pushing of the elevation part 200 is more stable.

[0060] Moreover, to facilitate the screwing of the push rod 700, a side grip rod can be arranged at the lower part of the threaded push rod 700, and the side grip rod extends radially. In this way, the side grip rod is convenient for hand - holding and more convenient for screwing the threaded push rod 700.

[0061] In another solution, the lower part of the threaded push rod 700 still has an external thread, and through the external thread, it can be directly matched with the threaded hole on the turntable of the centrifuge, so that the entire centrifuge tube 100 can be directly fixed on the centrifuge through the threaded push rod 700.

[0062] Embodiment III

[0063] As Figure 5 shown, based on the same concept, on the basis of Embodiment I, the centrifuge tube 100 is improved, and the elevation part 200 can be more conveniently taken out of the centrifuge tube 100.

[0064] The structure of this embodiment is as follows: at the bottom of the centrifuge tube 100, a height adjustment assembly 900 is provided. An adjusting screw 910 is rotatably provided at the bottom of the centrifuge tube 100, and the adjusting screw 910 extends into the inner cavity of the centrifuge tube 100. A bearing block 920 is movably provided in the inner cavity of the centrifuge tube 100, and the bearing block 920 slides axially in the inner cavity of the centrifuge tube 100. An internal threaded hole 921 is provided on the bearing block 920, and it is connected to the adjusting screw 410 through the internal threaded hole 921; a guiding groove 922 is opened on the side wall of the bearing block 920, and a guiding block 930 is opened on the side wall of the inner cavity of the centrifuge tube 100. The guiding groove 922 is sleeved on the guiding block 930 and slides axially through the guiding of the guiding block 930. And the elevation part 200 is arranged on the upper surface of the bearing block 920.

[0065] As Figure 5 shown, when the adjusting screw 910 is screwed, the bearing block 920 is driven to move axially through the internal threaded hole 921. In this way, the bearing block 920 is equivalent to the bottom of the centrifuge tube 100, and the depth of the centrifuge tube 100 is adjusted by adjusting the bearing block 920. In this way, a single elevation part 200 can be used to match micro-needle molds 500 of different heights, improving the practicality. Moreover, when the elevation part 200 needs to be taken out, the adjusting screw 910 can be directly screwed, and the elevation part 200 is pushed to move upward by the upward movement of the bearing block 920, so that the elevation part 200 can be easily taken out.

[0066] Embodiment IV

[0067] Based on the same concept, an embodiment of the present invention also proposes a micro-needle manufacturing method, wherein, for the micro-needle mold fixing device described in any one of the above, the micro-needle manufacturing method includes the steps of:

[0068] Step S10: Prepare a micro-needle precursor solution.

[0069] Step S20: Take an appropriate amount of solution and inject it into the micro-needle mold.

[0070] Step S30: Put it into the micro-needle mold fixing device and perform a centrifugation operation.

[0071] Specifically: First, place the elevation part into the centrifuge tube. Then, use tweezers to place the microneedle mold filled with the microneedle precursor solution into the centrifuge tube and place it on the elevation part. Next, place the sealing part into the centrifuge tube and cover the lid (the elevation part and the sealing part can be directly placed into the centrifuge tube by hand, and the centrifuge tube cap is tightened by hand). Finally, place the microneedle mold fixing device including the centrifuge tube into the centrifugation equipment for centrifugation operation.

[0072] Step S40: Dry.

[0073] Step S50: Take out the microneedle mold from the microneedle mold fixing device, and take out the formed microneedles from the microneedle mold.

[0074] Specifically, in the step of placing the microneedle mold fixing device for centrifugation operation: the centrifugation speed of the centrifuge is 3000 rpm, and the centrifugation time is 5 minutes. This allows the solution to fully fill the master mold.

[0075] Specifically, in the drying step, the drying temperature is 30°C, and the standing time is 8 hours.

[0076] The microneedles obtained by the microneedle manufacturing method of this embodiment are as Figure 3 shown.

[0077] In summary, different specifications of microneedle molds can be fixed between the elevation part and the sealing part, which has universality. The size and height of the elevation part and the sealing part can be made according to the size of the microneedle mold and the centrifuge tube. The materials are convenient to obtain, the process is simple, the matching degree with the microneedle mold and the centrifuge tube is high, the fixation is stable, so that the colloid is not easy to overflow, and the microneedle mold will not shake and cause imbalance during the centrifugation process; in addition, the structure of this microneedle mold fixing device is simple, the cost is low, the elevation part and the sealing part are easy to be removed and separated from the centrifuge tube, which is convenient for cleaning and can be reused. Therefore, it avoids the problems of inconvenient picking and placing, unstable structure, easy imbalance and liquid overflow in the prior art.

[0078] It should be understood that the application of the present invention is not limited to the above examples. For those of ordinary skill in the art, improvements or transformations can be made according to the above description. All these improvements and transformations should fall within the protection scope of the appended claims of the present invention.

Claims

1. A micro-needle mold fixing device based on centrifugal operation, characterized in that include: A centrifuge tube, wherein the centrifuge tube has an inner cavity; A padding portion, which can be detachably disposed in the inner cavity and is used to carry the microneedle mold, wherein the padding portion is a plastic padding portion, and the upper surface of the plastic padding portion abuts against and fits against the lower surface of the microneedle mold through elastic deformation; A sealing part, which is detachably located in the inner cavity and covers the opening of the microneedle mold, wherein the lower surface of the sealing part is in contact with the opening surface of the microneedle mold, thereby sealing the inner cavity of the microneedle mold, so that the impression material is sealed in the inner cavity of the microneedle mold and is molded through the inner cavity of the microneedle mold; a pulling portion, the pulling portion being connected to a surface of the sealing portion facing away from the raised portion; The outer wall of the raised portion and the outer wall of the sealing portion are both against the inner wall of the inner cavity; An adjusting screw is rotatably arranged at the bottom of the centrifuge tube, and the adjusting screw extends into the inner cavity of the centrifuge tube. A bearing block is movably arranged in the inner cavity of the centrifuge tube, and the bearing block slides axially in the inner cavity of the centrifuge tube. An internal threaded hole is arranged on the bearing block, and the bearing block is connected with the adjusting screw through the internal threaded hole; a guide groove is opened on the side wall of the bearing block, and a guide block is arranged on the side wall of the inner cavity of the centrifuge tube, and the guide groove is sleeved on the guide block, and slides axially by the guidance of the guide block, and the padding portion is arranged on the upper surface of the bearing block; The depth of the centrifuge tube is adjusted by adjusting the supporting block. Not only can the microneedle molds of different heights be matched with one raising portion, but also when the raising portion is to be taken out, the adjusting screw can be directly screwed, and the raising portion can be pushed upward by the upward movement of the supporting block to be taken out.

2. The micro-needle mold fixing device based on centrifugal operation according to claim 1, characterized in that, The pulling portion comprises: a screw, the screw being screwed on the sealing portion; A pull rope is connected to the screw.

3. The micro-needle mold fixing device based on a centrifugal operation according to claim 1, wherein, The sealing portion is made of an elastomeric material.

4. The micro-needle mold fixing device based on a centrifugal operation according to claim 3, characterized in that, The sealing part is formed by uniformly mixing an adhesive and a curing agent and curing them.

5. The micro-needle mold fixing device based on a centrifugal operation according to claim 3, characterized in that, The microneedle mold fixing device also includes a cover body, which is screwed onto the centrifuge tube and seals the inner cavity.

6. The micro-needle mold fixing device based on centrifugal operation according to claim 1, characterized in that The height of the raised portion+the height of the microneedle mold+the height of the sealing portion is less than or equal to the height of the centrifuge tube.

7. A method for manufacturing microneedles, characterized in that, For use in the microneedle mold fixing device according to any one of claims 1 to 6, the microneedle manufacturing method comprises the steps of: preparing a microneedle precursor solution; Take an appropriate amount of solution and inject it into the microneedle mold; Place the microneedle mold in the fixing device for centrifugation; dry; The microneedle mold is removed from the microneedle mold fixture, and the molded microneedles are removed from the microneedle mold.

8. The method for manufacturing microneedles according to claim 7, characterized in that, In the step of placing the microneedle mold into the fixing device for centrifugation: The centrifugal speed was 3000 rpm and the centrifugal time was 5 minutes; In the drying step, the drying temperature is 30°C and the standing time is 8 hours.

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