A soil compaction lawn growth-promoting bactericide and its application

Abstract

The present invention belongs to the technical field of microbial inoculant preparation, and specifically relates to a soil-compacted lawn growth-promoting inoculant and its application. A soil-compacted lawn growth-promoting inoculant provided by the present invention comprises the following components in parts by weight: 100 to 400 parts of yeast powder and 100 to 400 parts of glucose. The yeast decomposes glucose to produce carbon dioxide, which can comprehensively loosen the turf bed soil, greatly increase the soil porosity, enhance the soil's water and fertilizer retention capacity, promote the absorption of water and fertilizer by turfgrass, and thus promote the growth of turfgrass. The results of the examples show that after 14 days of comparing the comprehensive quality scores of the lawns using the soil-compacted lawn growth-promoting inoculant of the present invention, the treatment group scored 7 points (on a 9-point scale), and the control group scored 4 points (on a 9-point scale). It can be seen that after the microbial inoculant of the present invention is applied to turfgrass with poor growth in soil-compacted soil, it can better loosen the soil and rapidly and significantly improve the lawn quality.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial agent preparation, and in particular relates to a compacted soil lawn growth-promoting agent and application thereof. Background Art

[0002] Lawns have the function of greening and improving the ecological environment. They are becoming more and more closely related to humans and have become an indispensable part of human leisure activities and sports. They are involved in almost all outdoor places for human activities, including parks, stadiums, residential areas, schools, roadsides and scenic spots. Soil compaction is an important factor affecting the quality of lawns, which makes the soil air permeability poor, the growth of lawn grass difficult, the quality of lawns reduced, and even bare ground appears. The main cause of lawn soil compaction is high-density trampling activities by humans. In addition, heavy rain erosion, excessive irrigation and vehicle rolling can also cause lawn soil compaction and reduce lawn quality.

[0003] At present, there are mainly the following methods to solve the problem of low-quality lawns in compacted soil without replacing the turf: using mechanical drilling, striping, puncturing, surface loosening and vertical pruning. The above methods have been widely used in production and have achieved remarkable results, but there are also shortcomings: First, due to the need to maintain the functional integrity of the lawn, the lawn cannot be destroyed on a large scale, so the soil cannot be loosened comprehensively; second, relying on agricultural machinery is not suitable for lawns with small areas or lawns with complex environmental conditions. These lawns can only rely on manual operation, and the lawn quality recovery time is long, and it is difficult to quickly achieve lawn quality recovery. Therefore, it is necessary to develop new lawn soil loosening technology to quickly restore high-quality lawns without negatively affecting the ornamental value and use value of the lawn. Summary of the invention

[0004] The purpose of the present invention is to provide a compacted soil lawn growth-promoting bacterial agent, which has low cost, simple operation, no negative impact on the ornamental value and use value of the lawn, and can quickly restore dark green, dense and high-quality lawns.

[0005] The present invention provides a compacted soil lawn growth-promoting bacterial agent, characterized in that it comprises the following components in parts by weight: 100 to 400 parts of yeast powder and 100 to 400 parts of glucose;

[0006] The enzyme activity of the yeast powder is 20 billion viable bacteria / g.

[0007] Preferably, the following components are included in parts by weight: 200 parts of yeast powder and 400 parts of glucose.

[0008] The present invention provides the use of the lawn growth-promoting bacterial agent described in the above technical solution in improving lawn quality.

[0009] The present invention provides the application of the lawn growth-promoting microbial agent described in the above technical solution in loosening soil.

[0010] Preferably, during the application, the lawn growth-promoting microbial agent is mixed with water and sprayed on the soil to be improved.

[0011] Preferably, the application includes the following steps: the yeast powder is mixed with the first part of water to obtain a yeast cell suspension, which is sprayed on the soil for the first time; then the glucose is mixed with the second part of water to obtain a glucose solution, which is sprayed on the soil for the second time; finally, the third part of water is sprayed on the surface of the turfgrass for the third time.

[0012] Preferably, the viable count of the yeast cell suspension is 6 - 20 billion / mL; the mass concentration of the glucose solution is 8% - 16%.

[0013] Preferably, the time for the first spraying, the second spraying, and the third spraying is all during the vigorous growth season of the turfgrass.

[0014] Preferably, the total application amount of the yeast powder and glucose in the lawn growth-promoting microbial agent is 200 - 800 g / m 2 .

[0015] Preferably, 100 - 400 g of yeast powder and 100 - 400 g of glucose are sprayed per square meter.

[0016] The beneficial effects of the present invention: A compacted soil lawn growth-promoting microbial agent provided by the present invention includes the following components in parts by weight: 100 - 400 parts of yeast powder and 100 - 400 parts of glucose. The yeast decomposes glucose to produce carbon dioxide, which can comprehensively loosen the turfbed soil, greatly increase the soil porosity, enhance the soil's water and fertilizer retention capacity, promote the absorption of water and fertilizer by the turfgrass, and thus promote the growth of the turfgrass. The specific reaction equations are as follows:

[0017] The aerobic respiration reaction equation is: C6H 12 O6 (glucose) + 6H2O (water) + 6O2 (oxygen) → 6CO2 (carbon dioxide) + 12H2O (water) + energy;

[0018] The anaerobic respiration reaction equation is: C6H 12 O6 (glucose) → 2C2H5OH (alcohol) + 2CO2 (carbon dioxide) + energy.

[0019] The results of the examples show that, when comparing the comprehensive quality scores of the lawns after 14 days of applying the soil-compacted lawn growth-promoting microbial agent of the present invention, the treatment group scored 7 points (on a 9-point scale), and the control group scored 4 points (on a 9-point scale). It can be seen that after the microbial agent of the present invention is applied to the lawn grass with poor growth in soil-compacted soil, it can better loosen the soil, rapidly and significantly improve the lawn quality, significantly increase the chlorophyll content and density of the lawn grass, is easy to operate, does not rely on agricultural machinery operation, has low cost, and has no negative impact on the ornamental value and use value of the lawn. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required to be used in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0021] Figure 1 Growth states of the lawn grass in Example 1 and Comparative Example 1 after 7 days of treatment;

[0022] Figure 2 Growth states of the lawn grass in Example 1 and Comparative Example 1 after 14 days of treatment;

[0023] Figure 3 Growth states of the lawn grass in Example 2 and Comparative Example 3 after 14 days of treatment;

[0024] Figure 4 Soil volume change before and after 24 h of treatment with the microbial agent in Example 3. Figure 4 In this figure, CK represents before the treatment in Example 3, and "+" represents after the treatment with the microbial agent in Example 3. DETAILED DESCRIPTION OF THE INVENTION

[0025] The present invention provides a soil-compacted lawn growth-promoting microbial agent, which comprises the following components in parts by weight: 100 - 400 parts of yeast powder and 100 - 400 parts of glucose; the enzyme activity of the yeast powder is 20 billion viable bacteria per gram.

[0026] In the present invention, by weight, the lawn growth-promoting microbial agent provided by the present invention comprises 100 - 400 parts of yeast powder, further preferably 150 - 300 parts, and more preferably 200 parts; the enzyme activity of the yeast powder is 20 billion viable bacteria per gram. In the present invention, there is no special limitation on the source of the yeast powder, and conventional commercially available yeast powder or self-made yeast powder can be used. In the present invention, there is no special limitation on the method for making the self-made yeast powder, and the well-known preparation methods in the art can be adopted.

[0027] In the present invention, the yeast in the yeast powder decomposes glucose to produce carbon dioxide, which can comprehensively loosen the turf soil, greatly increase the soil porosity, enhance the soil's water retention and fertilizer storage capacity, promote the absorption of water and fertilizer by turfgrass, and thus promote the growth of turfgrass.

[0028] By weight, the turf growth-promoting bacterial agent provided by the present invention comprises 100 - 400 parts of glucose, more preferably 200 - 400 parts, and even more preferably 400 parts. In the present invention, there is no special limitation on the source of the glucose, and conventional commercially available products in the aquaculture industry can be used. In the present invention, the glucose promotes the growth and reproduction of yeast, is beneficial to the growth and reproduction of turfgrass, improves the soil structure, and makes the effect of loosening the soil better.

[0029] The present invention also provides the application of the turf growth-promoting bacterial agent described in the above technical solution in improving the quality of turf.

[0030] The present invention also provides the application of the turf growth-promoting bacterial agent described in the above solution in loosening the soil. In the present invention, when applying, it is preferably to mix the turf growth-promoting bacterial agent with water and spray it on the soil to be improved. In the present invention, there is no special limitation on the source of the water, and ordinary tap water can be used. In the present invention, the water can promote the yeast powder and glucose to penetrate deeply into the soil and be absorbed by the soil. When the turf growth-promoting bacterial agent does not contain water, it can be dissolved and sprayed with water in a corresponding proportion during actual application.

[0031] In the present invention, the application further preferably comprises the following steps: mixing the yeast powder with the first part of water to obtain a yeast suspension and spraying it on the soil for the first time, then mixing the glucose with the second part of water to obtain a glucose solution and spraying it on the soil for the second time, and finally spraying the third part of water on the surface of the turfgrass for the third time.

[0032] In the present invention, the viable count of the yeast suspension is preferably 6 - 20 billion / mL, more preferably 7 - 18 billion / mL, and even more preferably 8 - 16 billion / mL.

[0033] In the present invention, the mass concentration of the glucose solution is preferably 8% - 16%, more preferably 7.8% - 15%, and even more preferably 7.4% - 13.8%.

[0034] In the present invention, the mass ratio of the first part of water to the third part of water is preferably (2 - 1):1, more preferably (1.5 - 1):1, and even more preferably 1:1.

[0035] In the present invention, the times of the first spraying, the second spraying, and the third spraying are all preferably during the vigorous growth season of the lawn. In the present invention, the vigorous growth season of the lawn is preferably summer and August - September in autumn. In the present invention, the lawn growth - promoting microbial agent is sprayed into the soil, and the total application amount of the yeast powder and glucose of the lawn growth - promoting microbial agent is preferably 200 - 800 g / m 2 , and more preferably 300 - 700 g / m 2 , and even more preferably 600 g / m 2 . In the present invention, the lawn growth - promoting microbial agent is sprayed into the soil. When spraying, it is preferably to spray 100 - 400 g of yeast powder and 100 - 400 g of glucose per square meter, more preferably 100 - 400 g of yeast powder, 100 - 400 g of glucose and 5000 - 15000 mL of water, even more preferably to spray 150 - 300 g of yeast powder, 200 - 400 g of glucose and 6000 - 15000 mL of water per square meter, and still more preferably to spray 200 g of yeast powder, 400 g of glucose and 7000 mL of water per square meter. In the present invention, there is no special limitation on the addition amount of the water, as long as the water can prepare the yeast powder suspension and glucose solution to achieve uniform spraying of the yeast powder and glucose. In the present invention, the lawn growth - promoting microbial agent can be sprayed at any time period during the day without special limitation. In the embodiments of the present invention, the turfgrass is preferably Cynodon dactylon. The present invention has no special limitation on the turfgrass, and common turfgrasses can all be sprayed, and the optimal spraying amounts and optimal ratios for different turfgrasses are different.

[0036] In the present invention, the yeast is mixed with the first part of water to obtain a yeast suspension; the glucose is mixed with the second part of water to obtain a glucose aqueous solution. The yeast suspension and the glucose aqueous solution are absorbed by the soil and can penetrate deep into the soil. The carbon dioxide produced by the yeast decomposing glucose can comprehensively loosen the turf bed soil, greatly increase the soil porosity, increase the soil's water - retaining and fertilizer - retaining capacity, promote the absorption of water and fertilizer by the turfgrass, and thus promote the growth of the turfgrass.

[0037] To further illustrate the present invention, the following describes in detail a compacted - soil lawn growth - promoting microbial agent and its application provided by the present invention with reference to the drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0038] Example 1: Test in a greenhouse turnover box

[0039] The composition ratio of the compacted - soil lawn growth - promoting microbial agent is 200 parts by mass of yeast powder and 400 parts by mass of glucose per square meter of lawn. Specifically when spraying, each turnover box uses 20 g of yeast powder, 40 g of glucose and 1500 mL of tap water, and the lawn area in the turnover box is 0.1 m 2。The turfgrass in the turnover box is Cynodon dactylon.

[0040] During the peak growth season in summer, the Cynodon dactylon turf in the turnover box has formed a turf, and the growth states are consistent. After unified pruning, bricks are used to uniformly press the turf. The turf and the soil surface sink significantly until they can no longer sink. During this period, the same force is used to uniformly press 100 times. After pressing, a microbial agent treatment is carried out. Each turnover box is operated according to the following 3 steps:

[0041] (1) Weigh 20 g of yeast powder and mix it with 500 mL of the first part of water to obtain a yeast suspension, and pour it into the compacted soil of the Cynodon dactylon turf in a turnover box. The mass ratio of yeast powder to the first part of water is 1:25, and the viable count of the yeast suspension is 800 million / mL. The viable count of the yeast powder is 20 billion / g.

[0042] (2) Weigh 40 g of glucose and mix it completely with 500 mL of the second part of water, and then pour it into the compacted soil of the Cynodon dactylon turf in the same turnover box. The mass concentration of the glucose solution after mixing glucose and water is 7.4%.

[0043] (3) Uniformly spray 500 mL of the third part of water on the surface of Cynodon dactylon to wash away the residual yeast suspension and glucose solution on the surface of Cynodon dactylon, so that they can fully penetrate into the soil.

[0044] Example 2 Field test

[0045] The test on the use effect of this microbial agent is carried out in a compacted turf production base. The composition ratio of the microbial agent for promoting the growth of turf in compacted soil is 200 parts by mass of yeast powder, 400 parts by mass of glucose, and 7000 parts by mass of water. The total area of the test field is 300 m 2 , specifically during spraying, 200 g of yeast powder, 400 g of glucose, and 7000 mL of water are used per square meter of Cynodon dactylon turf. Specifically during application, prepare the total amount required for 300 m 2 of the turf at one time and then spray. The turfgrass is Cynodon dactylon.

[0046] When applying the microbial agent for promoting the growth of turf in compacted soil, just complete the following 3 steps in sequence:

[0047] Weigh 60 Kg of yeast powder and mix it evenly with 750 L of water to obtain a yeast suspension, and uniformly pour it into the turf of the compacted soil at a rate of 2.5 L per square meter. The mass ratio of yeast powder to the first part of water is 1:12.5, and the viable count of the yeast suspension is 1.6 billion / mL. The viable count of the yeast powder is 20 billion / g.

[0048] Weigh 120 Kg of glucose and mix it completely with 750 L of water, and uniformly pour it into the turf of the compacted soil at a rate of 2.5 L per square meter. The mass concentration of the glucose solution after mixing glucose and water is 13.8%.

[0049] 300m 2 Spray 600 L of water on the surface of the turfgrass, and evenly spray and rinse the yeast suspension and glucose solution on the surface of the bermudagrass turfgrass at a rate of 2 L per square meter, so that it fully penetrates into the soil.

[0050] Indoor experiment on loosening soil in Example 3

[0051] Experiment on the effect of the soil compaction turf growth promoter on the soil volume in a beaker.

[0052] Conduct an indoor experiment on loosening soil using the soil compaction turf growth promoter of Example 1. Select the soil from the same source as in Example 1, and weigh 1100 g of soil into a 1000 mL beaker. According to the soil area in the beaker, that is, the inner diameter area of the beaker (0.00865 m 2 ), and the dosage in Example 1 in the greenhouse turnover box experiment, calculate the dosage of the raw materials of the soil compaction turf growth promoter of the present invention, that is, 1.73 g of yeast powder, 3.46 g of glucose, and 130 mL of tap water. Before treatment, repeatedly hit the soil with a heavy object to compact the soil until it reaches the same scale in the beaker, that is, the same soil volume. Then use the agent of the present invention to treat the soil in the beaker.

[0053] Complete the following two-step operation treatment in sequence. Observe every day until the soil volume in the beaker no longer changes after 24 h: (1) Weigh 1.73 g of yeast powder and mix it evenly with 65 mL of water to obtain a yeast suspension, and pour it into the beaker soil. (2) Weigh 3.46 g of glucose and completely dissolve it with 65 mL of water, and then pour it into the beaker soil.

[0054] Comparative Example 1 Greenhouse turnover box experiment

[0055] The bermudagrass turf to be used has the same growth state, specifications and treatment method as the bermudagrass turf in Example 1. Only pour 1500 mL of tap water, without applying yeast powder and glucose, and the other steps are the same as in Example 1.

[0056] Comparative Example 2 Greenhouse turnover box experiment

[0057] The bermudagrass turf to be used has the same growth state, specifications and treatment method as the bermudagrass turf in Example 1. Calculated according to each turnover box of turf, the composition ratio of the soil compaction turf growth promoter is: 20 g of yeast powder, 100 g of glucose, and 1500 mL of tap water, and the other operation steps are the same as in Example 1.

[0058] Comparative Example 3 Field experiment

[0059] In the same lawn production base, select the compacted soil lawn adjacent to and with the same conditions as in Example 2, and only pour 7000 mL of tap water, without applying yeast powder and glucose, and the other steps are the same as in Example 2.

[0060] Indoor test on loose soil of Comparative Example 4

[0061] The measurement steps of the indoor test on loose soil are the same as those of Example 3, except that the compacted soil lawn growth promoter used is different. The composition ratio of the compacted soil lawn growth promoter used in the beaker of Comparative Example 4 is 1.73 g of yeast powder, 8.3 g of glucose, and 130 mL of tap water. The remaining steps are the same as those of Example 3.

[0062] Application Example 1: Experiment in turnover box

[0063] Analyze the growth conditions and turf establishment time of the turfgrass in Example 1, Comparative Example 1, and Comparative Example 2. For the growth conditions of the turfgrass, that is, the comprehensive quality of the turf, a scoring system is adopted. There are 3 observers, and each person scores independently. Finally, the average of the 3 observed values is used. When scoring, 9 points is the best and 1 point is the worst, and integers are taken when scoring. The comprehensive quality scoring standard is shown in Table 1.

[0064] Table 1 Comprehensive quality grading table

[0065]

[0066]

[0067] Monitor the turf establishment time. When the turfgrass coverage reaches 85%, it can be considered that the turf is established. The results of the growth conditions and turf establishment time of the turfgrass are as follows. Use a SPAD502 chlorophyll content meter to measure the relative chlorophyll content of bermudagrass in Example 1 and Comparative Example 1, and the results are as follows.

[0068] Before applying the compacted soil lawn growth promoter of the present invention, the turfgrass in Example 1 grew poorly. After applying the compacted soil lawn growth promoter of the present invention, the turf quality can be rapidly and significantly improved, and the chlorophyll content and density are significantly increased.

[0069] The turf establishment time of the turfgrass in Example 1 is 7 days, while that in Comparative Example 1 is 14 days, with a difference of 1 time. The growth states of the turfgrass in Example 1 and Comparative Example 1 after 7 days of treatment are shown in Figure 1 ; After 7 days, for the comparison of the comprehensive quality scores of the turf, Example 1 is 5 points and Comparative Example 1 is 1 point (on a 9-point scale, 9 points is the highest). After 14 days in Example 1, a high-quality turf with dark green and dense appearance can be restored, while in Comparative Example 1, it is still a low-quality turf with light green and sparse appearance and slow growth. The average value of the relative chlorophyll content of bermudagrass in Example 1 is 24.78 SPAD, and the average value of the relative chlorophyll content of bermudagrass in Comparative Example 1 is 8.98 SPAD. The growth states of the turfgrass in Example 1 and Comparative Example 1 after 14 days of treatment are shown in Figure 2 . After 14 days, for the comparison of the comprehensive quality scores of the turf, Example 1 is 7 points and Comparative Example 1 is 4 points.

[0070] After treating the turfgrass with poor growth in the compacted soil using the microbial agent of Comparative Example 2, when the dosage is too high, although more carbon dioxide can be produced and the soil can be better loosened, it will also cause growth stress to the turf plants. In Comparative Example 2, the excessive dosage of glucose led to yellowing of the turfgrass leaves and death of the above-ground parts, and new leaves did not grow until 14 days later.

[0071] Field experiment of Application Example 2

[0072] The density of the turfgrass in Example 2 was measured and a comprehensive quality score was given. The density measurement method was as follows: The number of turf plant branches in a 10 cm × 10 cm quadrat was calculated to represent the density. 14 days after the treatment with the microbial agent in Example 2, the turf in the treatment group was greener. After statistical analysis, the density increased significantly. The density of the microbial agent treatment group was 1.42 tiller branches / cm 2 , and the density of the control group was 0.63 tiller branches / cm 2 , and the density increased by 125.40%. Comparing the comprehensive quality scores of the turf after 14 days, Example 2 was 6 points and Comparative Example 3 was 4 points. The growth states of the turfgrass in Example 2 and Comparative Example 3 after 14 days of treatment are shown in Figure 3 .

[0073] The soil compaction conditions of Example 2 and Comparative Example 3 were analyzed using an SC900 soil compaction meter as follows: In Comparative Example 3, only tap water was irrigated, and the compactness at 5 cm and 10 cm depths of the compacted turf soil was 748.13 KPa and 896.38 KPa respectively. After applying the growth-promoting microbial agent for the compacted soil in Example 2, the compactness at 5 cm and 10 cm depths of the soil was 622.88 KPa and 803.13 KPa respectively. It can be seen that the soil compaction condition of Example 2 was changed, and the soil compactness at 5 cm and 10 cm depths of the soil was significantly reduced.

[0074] Indoor experiment on loosening soil of Application Example 3

[0075] The soil volumes of Example 3 and Comparative Example 4 were measured, and the soil volume in each beaker after treatment was obtained according to the scale on the beaker.

[0076] Percentage increase in soil volume = (soil volume of the treatment group - soil volume of the control group) * 100% / soil volume of the control group.

[0077] After treating Example 3 for 24 h, it was observed every day until the volume no longer changed. Compared with before the treatment, the soil volume in the beaker after the treatment increased significantly, with a volume increase of 1.49%, indirectly indicating that the soil porosity increased by 1.49%. The change in soil volume of Example 3 before and after 24 h of treatment with the microbial agent is shown in Figure 4 . The test results show that the microbial agent and its use method of the present invention can significantly increase the porosity of the compacted soil, thereby loosening the soil.

[0078] In Comparative Example 4, when using the bacterial agent at this dosage, the volume of the compacted soil in the beaker increased by 1.76%, that is, the porosity increased by 1.76%. Compared with Example 3, more soil porosity was increased, but the bacterial agent in Comparative Example 4 was not beneficial to the growth of Bermudagrass and would cause the death of Bermudagrass.

[0079] In summary, after the microbial bacterial agent of the present invention is applied to the turfgrass with poor growth in compacted soil, it can better loosen the soil, rapidly and significantly improve the turf quality, including significantly increasing the chlorophyll content and density, is simple to operate, does not rely on agricultural machinery operation, has low cost, and has no negative impact on the ornamental value and use value of the turf.

[0080] Although the above embodiments have made a detailed description of the present invention, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments according to these embodiments without creative efforts, and these embodiments all belong to the protection scope of the present invention.

Claims

1. Application of a lawn growth-promoting microbial agent in improving lawn quality, characterized in that, The lawn growth-promoting bactericide is composed of the following components in parts by weight: 200 parts of yeast powder and 400 parts of glucose; when applied, the lawn growth-promoting bactericide is mixed with water and sprayed on the soil to be improved; the application steps are as follows: the yeast powder is mixed with the first part of water to obtain a yeast suspension and sprayed on the soil for the first time, then the glucose is mixed with the second part of water to obtain a glucose solution and sprayed on the soil for the second time, and finally the third part of water is sprayed on the surface of the lawn grass for the third time; the viable count of the yeast suspension is 800 million to 1.6 billion / mL; the mass concentration of the glucose solution is 7.4% to 13.8%; the viable count of the yeast powder is 20 billion viable counts / g; the total application amount of the yeast powder and glucose in the lawn growth-promoting bactericide is 600 g / m 2 ; 200 g of yeast powder and 400 g of glucose are sprayed per square meter.

2. Application of a lawn growth-promoting microbial agent in loose soil, characterized in that, The lawn growth-promoting bactericide is composed of the following components in parts by weight: 200 parts of yeast powder and 400 parts of glucose; when applied, the lawn growth-promoting bactericide is mixed with water and sprayed on the soil to be improved; the steps of the application are as follows: the yeast powder is mixed with the first part of water to obtain a yeast suspension, which is sprayed on the soil for the first time, then the glucose is mixed with the second part of water to obtain a glucose solution, which is sprayed on the soil for the second time, and finally the third part of water is sprayed on the surface of the lawn grass for the third time; the viable count of the yeast suspension is 800 million to 1.6 billion / mL; the mass concentration of the glucose solution is 7.4% to 13.8%; the viable count of the yeast powder is 20 billion viable counts / g; the total application amount of the yeast powder and glucose in the lawn growth-promoting bactericide is 600 g / m 2 ; 200 g of yeast powder and 400 g of glucose are sprayed per square meter.

3. The application according to claim 1 or 2, characterized in that The times of the first spraying, the second spraying and the third spraying are all during the vigorous growth season of the lawn.

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