Contaminant paper chip detection device and methods of use and applications

Abstract

The application belongs to the field of pollutant detection, and particularly relates to a pollutant paper chip detection device, a use method and application. The pollutant paper chip detection device comprises a base, a paper chip automatic conversion device arranged on the base, a paper chip sample belt arranged on the paper chip automatic conversion device and a detection port. The paper chip automatic conversion device comprises a stepping motor, a driving wheel connected with the stepping motor and a driven wheel arranged on the base. The paper chip sample belt is arranged on the driving wheel and the driven wheel. The detection port is arranged correspondingly with the paper chip sample belt. The paper chip sample belt comprises a paper belt and paper chips uniformly arranged on the paper belt. The device improves the automation level of the analysis and detection instrument, simplifies the operation difficulty and increases the efficiency.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of pollutant detection, and particularly relates to a pollutant paper chip detection device and a use method and application thereof. BACKGROUND

[0002] The paper chip detection technology is a novel detection technology for qualitatively and quantitatively analyzing a detection sample on a specially treated paper-based chip, in combination with a fluorescent probe material (such as a luminescent carbon dot material) after excitation by light of a specific wavelength to produce a specific response.

[0003] The paper chip is cheap and easy to obtain, so that it can be widely applied to various detection means, but the detection of the treated sample needs to be combined with an optical detection device, and after sample feeding, a specific response fluorescent signal can be shown after irradiation by a light source, and during the detection process, the sample needs to be replaced manually, which results in low detection efficiency, large operation error and waste of human resources. SUMMARY

[0004] The application aims to overcome the defects in the prior art, and provides a pollutant paper chip detection device and a use method and application thereof.

[0005] To achieve the above-mentioned purpose, the following technical solutions are adopted in the application.

[0006] The application discloses a pollutant paper chip detection device, which comprises a base, a paper chip automatic conversion device arranged on the base, a paper chip sample belt arranged on the paper chip automatic conversion device, and a detection port.

[0007] Specifically, the base comprises a stepping motor mounting groove, a driving wheel limiting groove and a roller limiting groove; the roller limiting groove is used for limiting the driving wheel and the roller of the driven shaft.

[0008] Specifically, the distance between adjacent paper chips is 3.

[0009] Specifically, the stepping motor mounting groove is provided with a stepping motor limiting groove.

[0010] The base below the paper chip sample belt is provided with a sample pool; and the detection port is arranged in the sample pool.

[0011] The application also comprises a method for using the pollutant paper chip detection device, comprising the following steps:

[0012] S1: preparing a paper chip sample strip, designing a required pattern, and then printing the pattern by using a fluorescent carbon dot material as ink with an inkjet printer, punching a required number of detection paper chips on the filter paper printed with the carbon dots by using a punch, pasting the paper chips on sample holes cut by a punch on the hydrophobic sample strip, and completing the preparation of the paper chip strip;

[0013] S2: installing the pollutant paper chip detection device according to any one of claims 1-5, and fixing the paper chip sample strip obtained in step S1 on the driving wheel and the driven wheel;

[0014] S3: completing the detection by dripping the sample to be detected into the paper chip from the detection port.

[0015] The application also comprises an application of the pollutant paper chip detection device, which is applied to detecting Hg 2+ ; the fluorescent carbon dot material is prepared by using dopamine as a carbon source and o-phenylenediamine as a surface modifier by a hydrothermal method.

[0016] The filter paper is Whatman No. 4 filter paper.

[0017] The concentration of the carbon dot material in the ink is 40 μg / mL.

[0018] Compared with the prior art, the application has the following beneficial effects:

[0019] The pollutant paper chip detection device of the application can realize the automatic movement of the detection paper chip by driving the passive shaft by the driving wheel driven by the stepping motor, can control the sample feeding speed and direction, and can realize the rapid detection of multiple samples. The setting of the driving wheel limiting groove and the roller limiting groove ensures the stability and accuracy of the sample paper strip during the movement. The device has the characteristics of flexible design, high instrument adaptability, high reliability, easy high-throughput automatic detection, low operation difficulty, etc., improves the automation level of the analysis and detection instrument, greatly reduces the operation difficulty, and thus increases the efficiency.

[0020] In addition, the paper chip sample strip is used as a pollutant detection device, which integrates the inherent characteristics of the paper-based material, such as lightness, flexibility, low cost, easy processing, environmental protection, and disposable use, and can meet the needs of rapid environmental sample monitoring. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 FIG. 1 is a schematic diagram of the overall structure of the pollutant paper chip detection device of the application;

[0022] Figure 2Structure diagram of base of the application;

[0023] Figures 3-5 Fluorescence spectrum of different filter papers;

[0024] Figure 6 Fluorescence spectrum of carbon dot material ink of different concentrations (λex=532nm). DETAILED DESCRIPTION

[0025] In order to make the person skilled in the art better understand the technical solutions of the present application, the present application will be further described in detail below in combination with the drawings and the best embodiment.

[0026] Figures 1-2 A kind of pollutant paper chip detection device is shown, including base 1, paper chip automatic conversion device being arranged on base, paper chip sample strip 3 being arranged on the paper chip automatic conversion device and detection port 106;The paper chip automatic conversion device includes stepper motor 2, driving wheel being connected with the stepper motor and driven wheel being arranged on the base;The paper chip sample strip 3 is arranged on the driving wheel and the driven wheel;The detection port 106 is correspondingly arranged with the paper chip sample strip;The paper chip sample strip includes paper tape and paper chip being uniformly arranged on the paper tape.

[0027] The base includes stepper motor mounting groove 102, driving wheel limiting groove 101 and roller limiting groove 104;The roller limiting groove 104 is used to limit the roller 4 of driving wheel and driven shaft.The distance between adjacent paper chips is 3 roller limiting grooves.The stepper motor limiting groove 102 is arranged on the stepper motor mounting groove.The sample cell 105 is arranged on the base below the paper chip sample strip;The detection port 106 is arranged in the sample cell.

[0028] The paper chip sample strip 3 is fixed on the roller 5 of driving wheel and driven wheel, and then the roller 4 of driven wheel is combined with the center hole of roller 5 to form a sample carrying assembly, which is installed in the roller limiting groove 104, and then the roller of driving wheel is connected with the stepper motor 2, and the stepper motor is used to gradually push the driving wheel and the driven wheel to roll, under the limitation of sample cell 105, the paper chip sample strip 3 is sampled at a specified direction and speed, the sample is automatically sampled, and the operation skill requirement is reduced, which is convenient to operate.

[0029] Embodiment 1: a use method of the pollutant paper chip detection device, comprising the following steps:

[0030] S1: Prepare paper chip sample strips, design the required pattern, and then use an inkjet printer to make ink from fluorescent carbon dot material for pattern printing. Use a punch (5mm) on the printed filter paper to punch out the required number of detection paper chips. Paste the paper chips onto the sample holes cut by the punch on the hydrophobic sample strip, and the detection paper chip strip is completed. The sample hole spacing in this application is 2cm, and the hole diameter is 4mm.

[0031] S2: The pollutant paper chip detection device is installed, and the paper chip sample strip obtained in step S1 is fixed on the driving wheel and the driven wheel;

[0032] S3: The sample to be tested is dripped onto the paper chip from the testing port to complete the test.

[0033] Carbon dot paper chip printing conditions: A carbon dot material with fluorescent properties was synthesized using dopamine as a carbon source and o-phenylenediamine as a surface modifier (specifically, dopamine and o-phenylenediamine were mixed in a 2:1 ratio and added to a reaction vessel, then heated at 180°C for 16 hours). This material is resistant to Hg. 2+ It exhibits a specific response. Hg is immobilized via inkjet printing. 2+ For detection-type paper chips, parameters such as the choice of paper material and carbon dot concentration are crucial. Figures 3-5 As a paper-based chip material Figure 3 The image shows the fluorescence spectrum of a carbon dot paper chip on ordinary A4 printing paper. Figure 4 The fluorescence spectrum of the Whatman No. 3 filter paper carbon dot chip; Figure 5 The fluorescence spectrum of the Whatman No. 4 filter paper carbon dot paper chip is shown. Based on the comparison of the printing effects of the characteristic fluorescence peaks of the carbon dot materials, Whatman No. 4 filter paper was selected as the paper-based chip material. Furthermore, as... Figure 6 As shown, in order to obtain higher fluorescence intensity and prevent the precipitation of carbon dots in the ink under excessively high concentration conditions, 40 μg / mL was selected as the concentration of carbon dot material in the ink.

[0034] Batch testing of samples: Heavy metal mercury contaminants were detected using paper chips (Whatman No. 4 filter paper, carbon dot material concentration of 40 μg / mL). Batch testing experiments were conducted on four actual water samples to measure the recovery rate of mercury. 2+ Recovery rates ranged from 90.4% to 116%, with RSD < 3% (n = 3). Results are shown in Table 1.

[0035] Table 1

[0036]

[0037] The results show that the pollutant paper chip detection device of this application can achieve automated detection. Furthermore, by selecting the filter paper and the carbon point concentration, Hg detection can be achieved.2+ rapid detection.

[0038] The above merely provides the preferred embodiment of the present application and is not intended to limit the present application. Based on the idea of the present application, those skilled in the art can make modifications to the specific embodiments and applications without departing from the spirit and scope of the present application. The description herein is not intended to limit the present application.

Claims

1. An application of a contaminant paper chip detection device, characterized in that, Applied to the detection of Hg 2+ ; Specific response to Hg 2+ Fluorescent carbon dot materials were prepared by a hydrothermal method using dopamine as a carbon source and o-phenylenediamine as a surface modifier. The contaminant paper chip detection device includes a base, an automatic paper chip conversion device mounted on the base, a paper chip sample strip mounted on the automatic paper chip conversion device, and a detection port. The automatic paper chip conversion device includes a stepper motor, a driving wheel connected to the stepper motor, and a driven wheel mounted on the base. The paper chip sample strip is mounted on the driving wheel and the driven wheel. The detection port is correspondingly positioned to the paper chip sample strip. The paper chip sample strip includes a paper strip and paper chips evenly distributed on the paper strip. A sample pool is provided on the base below the paper chip sample strip. The detection port is located within the sample pool. The method of using the aforementioned contaminant paper chip detection device includes the following steps: S1: Prepare paper chip sample strips, design the required pattern, and then use an inkjet printer to print the pattern using fluorescent carbon dot material as ink. Use a punching machine to punch out the required number of detection paper chips on the printed filter paper. Paste the paper chips onto the sample holes cut by the punching tool on the hydrophobic sample strip, and the detection paper chip strip is completed. The filter paper is Whatman No. 4 filter paper; the concentration of carbon dot material in the printing ink is 40 μg / mL. S2: The pollutant paper chip detection device is installed, and the paper chip sample strip obtained in step S1 is fixed on the driving wheel and the driven wheel; S3: The sample to be tested is dripped onto the paper chip from the testing port to complete the test.

2. The application of the contaminant paper chip detection device according to claim 1, characterized in that, The base includes a stepper motor mounting slot, a drive wheel limiting slot, and a roller limiting slot; the roller limiting slot is used to limit the rollers of the drive wheel and the driven shaft.

3. The application of the contaminant paper chip detection device according to claim 2, characterized in that, The distance between adjacent paper chips is such that there are 3 roller limiting grooves.

4. The application of the contaminant paper chip detection device according to claim 2, characterized in that, The stepper motor mounting slot is provided with a stepper motor limiting slot.

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