Method for constructing a medium-flux single-cell copy number library and application thereof

Abstract

The application provides a medium-flux single-cell copy number library construction and sequencing method, which comprises the following steps: sorting single cells, independently performing cell lysis on each single cell, inserting genomic DNA by using a Tn5 transposase system, simultaneously performing single-cell-specific whole-genome barcode precise marking, then performing multi-sample mixing, performing multi-sample sequencing library construction in a single test tube, and then performing subsequent sequencing; and after sequencing, data corresponding to the identified cells are obtained by accurately decoding the single-cell sample barcode, and analysis is performed. The core of the application is that a Tn5 transposase containing a sample-specific nucleotide barcode oligonucleotide is used to fragment the single-cell sample DNA while adding a recognition sequence, then multiple samples are directly mixed at an early stage, so that pre-amplification is not required, early single-test-tube multi-sample operation is achieved, the library is compatible with a universal sequencing system, efficient genomic sequencing library construction and sequencing are achieved.

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