A method and system for measuring plant photosynthesis

Abstract

The present invention discloses a method and system for measuring plant photosynthesis, including: determining the distribution of leaf stomata; adhering a transparent adhesive member to the upper surface of the leaf, and after punching holes on the upper surface of the leaf according to the distribution of leaf stomata, fixing the leaf on a measuring table by the transparent adhesive member; making the leaf photosynthesize by shining light on the upper surface of the leaf, and detecting the concentration of photosynthesis products by providing a sensor on the measuring table. In the absence of a special leaf chamber, photosynthesis is measured with accurate measurement, low cost, convenience, good repeatability and accurate results.

Description

Technical Field

[0001] The present invention relates to the technical field of photosynthesis measurement, and in particular to a method and system for measuring plant photosynthesis. Background Art

[0002] The statements in this section merely provide background information related to the present invention and do not necessarily constitute prior art.

[0003] Photosynthesis refers to the process in which green plants (including algae) absorb light energy, synthesize carbon dioxide and water into energy-rich organic matter, and release oxygen at the same time. Traditional methods for measuring photosynthesis include the oxygen electrode method, the half-leaf method, and the CO2 variable method, which are complicated to operate and have poor accuracy. At present, photosynthesis measurement is mostly based on the CO2 variable method. Compared with traditional methods, the photosynthesis measurement system has accurate measurement, multiple measurement parameters, and a wide range of applications. The measurement system consists of a leaf chamber (such as an infrared gas analyzer), an operation console, and connecting cables. The leaf chamber can measure multiple photosynthetic indicators of plant leaves, including standard leaf chambers, such as red and blue leaf chambers, as well as needle leaf chambers, cluster leaf chambers, etc., but they are expensive. For plants with smaller leaves, or plants with peculiar leaf shapes such as needle plants and cluster plants, standard leaf chambers can also be used for measurement, but because the leaves are smaller, needle leaves and other special-shaped leaves cannot fill the leaf chamber. Affected by the clamping, the clamping will be too thick or insufficient, resulting in large changes in the measurement results, poor repeatability, large measurement errors, and even data cannot be used. Summary of the invention

[0004] In order to solve the above problems, the present invention proposes a method and system for measuring plant photosynthesis, which can measure photosynthesis without a special leaf chamber, with precise measurement, low cost, convenience, good repeatability and accurate results.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] In a first aspect, the present invention provides a method for measuring plant photosynthesis, comprising:

[0007] Determine the distribution of stomatal pores on leaves;

[0008] A transparent adhesive piece is attached to the upper surface of the leaf, and after punching holes on the upper surface of the leaf according to the distribution of the stomata of the leaf, the leaf is fixed on the measuring table by the transparent adhesive piece;

[0009] The leaves are made to photosynthesize by shining light on their upper surfaces, and the concentration of the photosynthesis product is detected by providing a sensor on the measuring platform.

[0010] As an optional implementation, a plurality of blades are spliced ​​on the same plane.

[0011] As an optional embodiment, a plurality of blades are arranged in a single layer in parallel in a certain direction.

[0012] As an optional implementation, the measuring platform is hollowed out, and the lower surface of the blade is exposed and arranged in the hollow part of the measuring platform.

[0013] As an optional embodiment, the sensor is a CO2 sensor to detect the concentration of CO2 after photosynthesis.

[0014] As an optional implementation, a distribution rate threshold is preset, and the piercing measures are determined based on a comparison between the stomata distribution rate of the blade and the distribution rate threshold.

[0015] As an optional implementation, if the pore distribution rate on the upper surface of the blade is less than or equal to the distribution rate threshold, holes are evenly pierced in the gaps between the blades.

[0016] As an optional implementation, if the pore distribution rate on the upper surface of the blade is greater than the distribution rate threshold, before fixing the blade, 2 to 5 pores / cm 2 Punch holes evenly in the gaps between blades.

[0017] As an optional implementation, the blade is a blade with an area smaller than a threshold, a blade with a width smaller than a threshold, or a needle-shaped blade.

[0018] As an optional embodiment, the width of the transparent adhesive member is greater than the width of the blade.

[0019] In a second aspect, the present invention provides a plant photosynthesis measurement system, comprising: a light source, a measurement platform, and a sensor disposed on the measurement platform;

[0020] A leaf is fixed on the measuring table, and the fixing process includes: determining the pore distribution of the leaf, adhering a transparent adhesive member to the upper surface of the leaf, and punching holes on the upper surface of the leaf according to the pore distribution of the leaf, and fixing the leaf on the measuring table by the transparent adhesive member;

[0021] The light source is used to illuminate the upper surface of the leaf so that the leaf can perform photosynthesis;

[0022] The sensor is used to detect the concentration of photosynthesis products.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention provides a plant photosynthesis measurement method and system, which uses transparent tape to adhere the upper surface of the leaf for planar fixation, thereby ensuring the stability of the leaf area, ensuring good gas exchange, and minimizing the influence of fixation on photosynthesis. The photosynthesis of leaves per unit area is further measured, and the data is stable and accurate with good repeatability. The photosynthesis of leaves with special shapes can be measured without a special leaf chamber, and the problem that a photosynthetic instrument cannot accurately measure the photosynthesis of small leaves, narrow leaves or coniferous plants without a special leaf chamber is effectively solved, and the problem of clamping small leaves by a standard leaf chamber is solved. The method has the advantages of simple operation and low cost.

[0025] Advantages of additional aspects of the present invention will be given in part in the following description, and in part will become obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0027] Figure 1 This is a schematic diagram of a method for measuring plant photosynthesis provided in Example 1 of the present invention. DETAILED DESCRIPTION

[0028] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0029] It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0030] It should be noted that the terms used herein are only for describing specific embodiments, and are not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that the terms "include" and "have" and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device comprising a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0031] In the absence of conflict, the embodiments of the present invention and the features of the embodiments may be combined with each other.

[0032] Example 1

[0033] Photosynthesis is the most important chemical reaction in nature. Common determination methods include oxygen electrode method, half leaf method and CO2 variable method. At present, photosynthesis determination is mostly based on CO2 variable method. The most common determination instrument is photosynthesis tester, and standard leaf chambers such as needle-shaped leaf chamber, cluster leaf chamber, duckbill leaf chamber can be selected for different leaf shapes. However, for plants with small leaves and special needle shapes, although the standard leaf chamber can be used for determination, it is affected by the clamping, and the clamping may be too thick or insufficient, resulting in large changes in the measurement results, poor repeatability, and low data quality.

[0034] Therefore, this embodiment provides a method for measuring plant photosynthesis, which measures leaf photosynthesis without a dedicated leaf chamber, such as Figure 1 As shown, including:

[0035] Determine the distribution of stomatal pores on leaves;

[0036] A transparent adhesive piece is attached to the upper surface of the leaf, and after punching holes on the upper surface of the leaf according to the distribution of the stomata of the leaf, the leaf is fixed on the measuring table by the transparent adhesive piece;

[0037] The leaves are made to photosynthesize by shining light on their upper surfaces, and the concentration of the photosynthesis product is detected by providing a sensor on the measuring platform.

[0038] Since the stomata of the leaves are mostly distributed on the lower surface of the leaves and are used to absorb carbon dioxide and carry out photosynthesis, and the upper surface of the leaves is mostly chloroplasts, which require light and will produce water and oxygen under light; therefore, in this embodiment, the lower surface of the leaves is exposed and arranged on the measuring table, and a transparent adhesive member is adhered to the upper surface of the leaves, and the leaves are fixed to the measuring table by the transparent adhesive member, and the stomata on the lower surface of the leaves carry out photosynthesis and will not be affected by the transparent adhesive member.

[0039] As an optional implementation, a plurality of blades are spliced ​​on the same plane.

[0040] Furthermore, the plurality of blades are arranged in a single layer in parallel in a certain direction.

[0041] As an optional implementation manner, the measuring platform is hollowed out, and the lower surface of the leaf is exposed in the hollowed-out portion of the measuring platform, and the concentration of the photosynthesis product is detected by a sensor arranged on the measuring platform.

[0042] Furthermore, the sensor is a CO2 sensor to detect the concentration of CO2 after photosynthesis.

[0043] In this embodiment, the stomata distribution of the leaves can be determined by querying data or actual measurement, a distribution rate threshold is preset, and the piercing measures are determined based on the comparison between the stomata distribution rate of the leaves and the distribution rate threshold.

[0044] Specifically, the distribution rate threshold is set to 5%. If the pore distribution rate on the upper surface of the blade is less than or equal to 5%, a transparent adhesive is used to adhere to the upper surface of the blade. When multiple blades are spliced ​​on the same plane, the gap between the blades is as small as possible and stacking is avoided. At the same time, holes are evenly pierced in the splicing gap.

[0045] If the pore distribution rate on the upper surface of the leaf is greater than 5%, before fixing the leaf, the pore distribution rate should be 2 to 5 / cm. 2 Punch holes to avoid pore blockage caused by adhesion, which makes gas exchange impossible; at the same time, holes can also be evenly punched in the gaps between blades.

[0046] In this embodiment, the blade is a small blade whose area is smaller than a threshold value, or a small blade or a needle-shaped blade whose width is smaller than a threshold value.

[0047] As an optional embodiment, the blade has an area less than 6 cm 2 Small leaves, or leaves less than 2 cm wide.

[0048] As an optional implementation manner, the transparent adhesive member is a transparent tape with as high a transparency as possible.

[0049] As an optional implementation manner, the width of the transparent adhesive member is greater than the width of the blade.

[0050] In this embodiment, the measurement process can be carried out according to the measurement rules of the photosynthetic instrument, or a standard leaf chamber can be used for measurement, and the leaves are fixed in the standard leaf chamber according to the above scheme to solve the problem of the standard leaf chamber clamping the leaves; this is not limited here;

[0051] Through the measurement process, the strength of plant photosynthesis, transpiration, water utilization, etc. can be judged, and the plant growth and adaptability to the environment can also be judged.

[0052] In this embodiment, the upper surface of the leaf is adhered with a transparent tape to perform a planar fixation, thereby ensuring the stability of the leaf area, ensuring good gas exchange, minimizing the effect of fixation on photosynthesis, and further measuring the photosynthesis of the leaf per unit area. The data is stable and accurate, with good repeatability, and the photosynthesis of leaves with special shapes can be measured without a special leaf chamber. This effectively solves the problem that the photosynthetic instrument cannot accurately measure the photosynthesis of small leaves, narrow leaves or coniferous plants without a special leaf chamber, and has the advantages of simple operation and low cost.

[0053] In this example, photosynthesis related to Fraxinus chinensis, Malus chinensis, Pyrus betulaeformis, Pinus maritime, etc. was measured. Net photosynthesis (Pn), stomatal conductance Gs, intercellular carbon dioxide concentration Ci, transpiration rate Tr and other indicators were measured to evaluate the impact of the method of this example on related parameters, and at the same time evaluate the impact of scotch tape on photosynthesis.

[0054] Table 1 Effect of the method of this embodiment on photosynthetic parameters

[0055] Pn Gs Ci Tr The method of this embodiment 6.47±1.36 0.1087±0.231 289±21 2.16±0.17 Control (CK) 6.51±1.29 0.1091±0.251 292±25 2.17±0.21

[0056] Photosynthesis was measured on plants with larger leaves, which were attached with transparent tape. The control was a natural leaf. The same leaf was selected for treatment and a t-test was used for consistency test (n=10). At the 95% confidence interval, the two methods did not reach a significant level and the difference between the two was not statistically significant. It is believed that both methods can be used for photosynthesis measurement.

[0057] In addition, the two methods were used to measure the photosynthesis of small-leaved plants (Xinjiang Fraxinus chinensis) and near-needle plants (Tamarix chinensis). The results are shown in Table 2. The measurement method is to select the same bunch of leaves to measure the photosynthesis five times in a short period of time, and the light source is natural light. The stability and reliability of the measurement are evaluated.

[0058] Table 2 Net photosynthesis determination table of Fraxinus chinensis and Tamarix chinensis

[0059]

[0060] Since many parameters are involved, only net photosynthesis is analyzed. It can be seen that the method of this embodiment has good stability in measuring photosynthetic data, with a coefficient of variation not exceeding 10%. The coefficient of variation for direct measurement is large, both exceeding 25%, and the data consistency is poor. Other data also show similar characteristics. Therefore, the method of this embodiment has the advantages of good repeatability and accurate data.

[0061] Example 2

[0062] This embodiment provides a plant photosynthesis measurement system, including: a light source, a measurement platform, and a sensor disposed on the measurement platform;

[0063] A leaf is fixed on the measuring table, and the fixing process includes: determining the pore distribution of the leaf, adhering a transparent adhesive member to the upper surface of the leaf, and punching holes on the upper surface of the leaf according to the pore distribution of the leaf, and fixing the leaf on the measuring table by the transparent adhesive member;

[0064] The light source is used to illuminate the upper surface of the leaf so that the leaf can perform photosynthesis;

[0065] The sensor is used to detect the concentration of photosynthesis products.

[0066] Although the above describes the specific implementation mode of the present invention in conjunction with the accompanying drawings, it is not intended to limit the scope of protection of the present invention. Those skilled in the art should understand that various modifications or variations that can be made by those skilled in the art on the basis of the technical solution of the present invention without creative work are still within the scope of protection of the present invention.

Claims

1. A method for measuring plant photosynthesis, characterized in that: include: Determine the distribution of stomatal pores on leaves; A transparent adhesive piece is attached to the upper surface of the leaf, and after punching holes on the upper surface of the leaf according to the distribution of the stomata of the leaf, the leaf is fixed on the measuring table by the transparent adhesive piece; The leaves are made to photosynthesize by shining light on the upper surface of the leaves, and the concentration of the photosynthesis product is detected by providing a sensor on the measuring platform; A distribution rate threshold is preset, and the piercing measures are determined based on the comparison between the stomatal distribution rate of the leaves and the distribution rate threshold; If the pore distribution rate on the upper surface of the leaf is less than or equal to the distribution rate threshold, holes are evenly punched in the gap between the leaf joints; If the pore distribution rate on the upper surface of the leaf is greater than the distribution rate threshold, before fixing the leaf, 2 to 5 pores / cm 2 Punch holes evenly in the gaps between blades; The blade is a blade with an area smaller than a threshold, a blade with a width smaller than a threshold, or a needle-shaped blade; The distribution rate threshold is 5%.

2. A method for measuring plant photosynthesis as claimed in claim 1, characterized in that: Join multiple blades on the same plane.

3. A method for measuring plant photosynthesis as claimed in claim 2, characterized in that: Multiple blades are arranged in a single layer in parallel in a certain direction.

4. A method for measuring plant photosynthesis as claimed in claim 1, characterized in that: The measuring platform is hollowed out, and the lower surface of the blade is exposed and arranged at the hollowed-out portion of the measuring platform.

5. A method for measuring plant photosynthesis as claimed in claim 1, characterized in that: The sensor is a CO2 sensor to detect the concentration of CO2 after photosynthesis.

6. A method for measuring plant photosynthesis as claimed in claim 1, characterized in that: The width of the transparent adhesive member is greater than the width of the blade.

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