A water hyacinth biological control strain, use method and application thereof

Abstract

The present invention belongs to the technical field of biological control of water hyacinth, and discloses a water hyacinth biological control strain, a method of use and its application. The water hyacinth biological control strain is Cercospora rodmanni J1, and the preservation information of the water hyacinth biological control strain J1 is GDMCCNo: 62031, 2021.11.9. The water hyacinth biological control strain derived from native water hyacinth-Cercospora rodmanni J1 and its preparation can inhibit the growth and reproduction of water hyacinth, have host specificity and biosafety, will not pollute the ecological environment of water bodies, and belong to the biological control technology scheme. The present invention can significantly improve the infection efficiency of the control bacteria agent by inducing surrounding insects to cause a certain amount of mechanical trauma to water hyacinth. The bacteria agent is spread by insect vectors, expands the scope of water hyacinth control and management, effectively inhibits the growth and reproduction of water hyacinth, and has no adverse effects on the water ecology and other plants in the water area, so that it can have a better management effect.

Description

Technical Field

[0001] The invention belongs to the technical field of water hyacinth biological control, and in particular relates to a water hyacinth biological control strain, a use method and an application thereof. Background Art

[0002] Nowadays, the invasion and proliferation of water hyacinth has become a global problem. Traditional physical control methods mainly rely on manual or mechanical salvage, which consumes a lot of manpower and material resources, while chemical control methods use chemical herbicides and other drugs that easily pollute the ecological environment. It has become a consensus in the scientific and technological community that biotechnology will become the most promising method for the prevention and control of water hyacinth, which is sustainable.

[0003] Through the above analysis, the problems and defects of the existing technology are: the traditional physical control method of water hyacinth consumes a lot of manpower and material resources, while the chemical control technology pollutes the ecological environment, and both are not sustainable. Summary of the invention

[0004] In view of the problems existing in the prior art, the present invention provides a water hyacinth biological control strain, a use method and applications thereof, and in particular relates to a water hyacinth biological control strain - Cercospora rodmanii use method and applications thereof.

[0005] The present invention is achieved by providing a water hyacinth biological control strain, wherein the water hyacinth biological control strain is Cercospora rodmanii J1.

[0006] Furthermore, the preservation information of the water hyacinth biological control strain J1 is GDMCC No: 62031, 2021.11.9.

[0007] Furthermore, the culture characteristic experiment of the water hyacinth biological control strain J1 showed that the most suitable carbon source for bacterial growth was glucose, the nitrogen source was glycine, and the optimal pH was 4.0.

[0008] Another object of the present invention is to provide a bacterial agent prepared by using the water hyacinth biological control strain, wherein the bacterial agent has the water hyacinth biological control strain J1 as the main component and the amount of the bacterial agent added to the water hyacinth is> 10 10 CFU / mu bacteria.

[0009] Furthermore, the emulsifier content of the bacterial agent is 5-10wt%, the glycine addition amount is 3-5g / L, the pH is adjusted to 4-6, and the applicable temperature is 15-40°C.

[0010] Furthermore, the method of using the bacterial agent is atomization spraying, and the best effect is achieved in hot and humid weather in summer, with an application cycle of once every 1 to 2 months.

[0011] Furthermore, the use of the bacterial agent also includes designing a solar insect attractant lamp mechanism.

[0012] Furthermore, the solar insect attractant lamp mechanism includes a solar electromagnetic panel, a battery, a waterproof black light, a fixing device and a connecting wire lamp; the solar electromagnetic panel stores electrical energy during the day and discharges it at night, and the black light is turned on to attract insects in the surrounding environment to gnaw on the leaves and stems of water hyacinth.

[0013] Furthermore, the use scope of the bacterial agent includes water hyacinth flooded areas in flowing water bodies and static rivers and lakes.

[0014] Another object of the present invention is to provide an application of the water hyacinth biological control strain in inhibiting the growth and reproduction of water hyacinth, as well as in water hyacinth biological control and water ecological management.

[0015] In combination with the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solutions to be protected by the present invention are analyzed from the following aspects:

[0016] First, in view of the technical problems existing in the above-mentioned prior art and the difficulty of solving the problems, the technical solutions to be protected by the present invention and the results and data during the research and development process are closely combined to analyze in detail and deeply how the technical solutions of the present invention solve the technical problems, and some creative technical effects brought about after solving the problems. The specific description is as follows:

[0017] The present invention provides a biological control strain: Cercospora rodmanii and a method for applying the same to water hyacinth treatment. The present invention provides a Cercospora rodmanii J1 (strain collection number GDMCC No: 62031), which is derived from my country, separated and screened from the water hyacinth region of the Fujian River Basin, and has the characteristics of strong adaptability, high specificity, and significant treatment effect. In order to further improve the biological control and treatment efficiency of water hyacinth, a method for using Cercospora rodmanii bacterial agent is invented: by setting a solar insect trapping lamp mechanism, turning it on at night, and inducing insects in the surrounding environment to gnaw on the leaves and stems of water hyacinth, water hyacinth plant trauma can be caused on a large scale, and the infection efficiency of the control bacterial agent can be significantly improved. The bacterial agent is spread by insect vectors, and the scope of water hyacinth control and treatment is expanded. At the same time, the present invention can cause the occurrence and prevalence of water hyacinth diseases on a large scale, effectively inhibit the reproduction of water hyacinth, and have no adverse effects on water ecology and other plants in water areas.

[0018] The present invention provides a water hyacinth biological control strain, Cercospora rodmanii J1, and its preparation, which can inhibit the growth and reproduction of water hyacinth, have host specificity and biosafety, will not pollute the water ecological environment, and belong to a biological control technical solution. Cercospora rodmanii J1 parasitizes on water hyacinth, destroys the tissues and cells of water hyacinth by competing with the water hyacinth host for nutrients, secreting toxins, etc., infects the leaves, stems, and roots of water hyacinth in the later stage, prompts the water hyacinth plant to wither and rot, and effectively causes the water hyacinth to lesion and necrosis to achieve the control effect of controlling reproduction. The present invention also utilizes the principle of insect ecology, designs a solar insect attracting lamp mechanism to lure surrounding insects to eat water hyacinth, reduces the insect pests of surrounding crops to a certain extent, and protects the local crop planting at the same time. The present invention can significantly improve the infection efficiency of the control bacteria agent by luring surrounding insects to cause a certain amount of mechanical trauma to water hyacinth, and the bacteria agent is spread by insect vectors, expands the scope of water hyacinth control and management, further inhibits the growth and reproduction of water hyacinth, and enables it to have a better management effect.

[0019] Second, considering the technical solution as a whole or from the perspective of the product, the technical effects and advantages of the technical solution to be protected by the present invention are described in detail as follows:

[0020] The present invention uses the key technology of local microorganisms to prevent and control invasive alien species, and provides technical support for the innovative strategies of water hyacinth management in the future. The present invention develops water hyacinth biocontrol bacteria by separating and screening conditionally pathogenic pathogenic microorganisms with strong specificity, and inhibits the growth and reproduction of water hyacinth or destroys the tissues and cells of water hyacinth by competing with the water hyacinth host for nutrients or infecting water hyacinth. In order to further improve the biological control and management efficiency of water hyacinth, the use method of Rodmanni Cercospora agent is invented: by setting a solar insect attracting lamp mechanism, turning it on at night, and inducing insects in the surrounding environment to gnaw on the leaves and stems of water hyacinth, it can cause large-scale water hyacinth plant trauma, significantly improve the infection efficiency of the control agent, promote the spread of the agent through insect vectors, expand the scope of water hyacinth control and management, and can cause the occurrence and prevalence of water hyacinth diseases on a large scale, effectively inhibit the reproduction of water hyacinth, and have no adverse effects on the water ecology and other plants in the water area.

[0021] The water hyacinth biological control strain J1 provided by the present invention is a water hyacinth originating from China, having host specificity, being a non-exotic species, and having high biosafety characteristics. Application of Cercosporarodmanii J1 in inhibiting the growth and reproduction of water hyacinth, as well as biological control of water hyacinth and water ecological management. The water hyacinth biological control strain J1 can cause water hyacinth to quickly develop diseases, causing the leaves and neck of water hyacinth to wither and rot, and the overall water hyacinth plant to be dwarfed; inhibiting the growth and reproduction speed of water hyacinth, reducing the density of water hyacinth, and improving the water ecology.

[0022] Third, as auxiliary evidence of the inventiveness of the claims of the present invention, it is also reflected in the following important aspects:

[0023] (1) The expected benefits and commercial value of the technical solution of the present invention after transformation are:

[0024] The invention can be converted into a commercial water hyacinth biological herbicide and has high commercial value.

[0025] (2) The technical solution of the present invention fills the technical gap in the industry at home and abroad:

[0026] At present, there is no commercialized or registered water hyacinth biological herbicide on the market. The water hyacinth biological herbicide transformed by the technical solution of the present invention will have independent intellectual property rights and can fill the gap in domestic water hyacinth ecological biological control technology.

[0027] (3) The technical solution of the present invention solves the technical problems that people have been eager to solve but have never been able to solve successfully:

[0028] Traditional physical control methods for water hyacinth mainly rely on manual or mechanical salvage, which consumes a lot of manpower and material resources, while chemical control methods use chemical herbicides and other drugs that easily pollute the ecological environment. The water hyacinth biological control technical solution of the present invention solves the technical problems that people have always been eager to solve, such as high water hyacinth control costs and environmental pollution, but have never been successful. The technical solution of the present invention not only reduces the labor cost of water hyacinth control, but is also mild to the environment and sustainable.

[0029] (4) The technical solution of the present invention overcomes technical prejudice:

[0030] With the improvement of global environmental awareness and the need for sustainable ecological development, efficient, environmentally friendly and harmless microbial herbicides are increasingly showing their important social significance and economic value. The present invention overcomes the technical prejudices of high cost, environmental pollution and low efficiency of biological treatment methods for water hyacinth by developing water hyacinth biological agents. The physiological growth characteristics and formula of the strain will solve the problems of stability and dependence on water of the biological herbicide, increase the affinity and adhesion to water hyacinth, and overcome the previous technical prejudices on low stability, low adhesion and slow effect of biological herbicides. On the basis of the technical solution of the present invention, the compound use of a low amount of plant growth regulator (dimethyltetrachloride sodium) can significantly improve the effect and efficiency of biological control of water hyacinth. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0032] Figure 1 It is a schematic diagram of the rodmanni Cercospora strain J1 obtained by separation and screening provided in an embodiment of the present invention.

[0033] FIG2 is a schematic diagram of the growth curve of the Cercospora rodmanii strain J1 provided in an embodiment of the present invention.

[0034] FIG. 2( a ) is a schematic diagram of a pH growth curve of the Cercospora rodmanni strain J1 provided in an embodiment of the present invention.

[0035] FIG2( b ) is a schematic diagram of the growth curve of the carbon source of the Cercospora rodmanii strain J1 provided in an embodiment of the present invention.

[0036] FIG2( c ) is a schematic diagram of the growth curve of the nitrogen source of the Cercospora rodmanii strain J1 provided in an embodiment of the present invention.

[0037] Figure 3 It is a schematic diagram of the principle of the solar insect trapping lamp mechanism and the insect media of the fungicide provided by the embodiment of the present invention;

[0038] In the figure: 1. Solar panels; 2. Insect-attracting lamps; 3. Transmission routes of insect vectors; 4. Sprayed bacteria; 5. Attracted insects (moths); 6. Symptoms of water hyacinth infected by bacterial agents; 7. Water hyacinth; 8. Insect-attracting area. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0040] 1. Explanatory Examples In order to enable those skilled in the art to fully understand how to implement the present invention, this section provides an illustrative example that expands and describes the technical solution of the claims.

[0041] In view of the problems existing in the prior art, the present invention provides a water hyacinth biological control strain, a use method and an application thereof. The present invention is described in detail below in conjunction with the accompanying drawings.

[0042] The water hyacinth biological control strain provided in an embodiment of the present invention has the preservation information: GDMCC No: 62031, 2021.11.9.

[0043] The water hyacinth biological control strain provided in the embodiment of the present invention is deposited in GDMCC, and the application of Cercospora rodmanii J1 with the deposit number of GDMCC No: 62031 in inhibiting the growth and reproduction of water hyacinth, as well as biological control of water hyacinth and water ecological management. It is deposited in the Guangdong Provincial Microbiological Culture Collection Center, and the address of the collection unit is Guangdong Provincial Institute of Scientific Microbiology, Building 59, 5th Floor, No. 100 Xianlie Middle Road, Guangzhou. The proposed taxonomic name is: Cercospora sp., which was received by the collection center on November 2, 2021 and registered. It will be preserved for thirty years from that date, and will be preserved for another five years after receiving a request for a biological material sample before the expiration. The viability of the biological material (strain) was tested by the collection center on November 4, 2021. The result is: survival.

[0044] The water hyacinth biological control strain J1 provided in the embodiment of the present invention is derived from the water hyacinth native to China, has host specificity, belongs to a non-alien species, and has a high biosafety characteristic.

[0045] The water hyacinth biological control strain J1 provided in the embodiment of the present invention can cause water hyacinth to quickly develop diseases, causing the leaves and neck of the water hyacinth to wither and rot, and the overall water hyacinth plant to dwarf; inhibit the growth and reproduction rate of water hyacinth, reduce the density of water hyacinth, and improve the water ecology.

[0046] The culture characteristic experiment of the water hyacinth biological control strain J1 provided in the embodiment of the present invention shows that the most suitable carbon source for the growth of the strain is glucose; the nitrogen source is glycine; and the optimal pH is 4.0.

[0047] The bacterial agent prepared by the water hyacinth biological control strain J1 provided in the embodiment of the present invention uses J1 as the main component and the amount of the bacterial agent added to the water hyacinth is> 10 10 CFU / mu of bacteria, wherein the added emulsifier content is 5-10wt%, the added amount of glycine is 3-5g / L, the pH is adjusted to 4-6, and the applicable temperature is 15-40 degrees Celsius.

[0048] The method for using the bacterial agent prepared by the water hyacinth biological control strain J1 provided in the embodiment of the present invention is atomization spraying. The application effect is best in high temperature and high humidity weather in summer, and the application cycle is once every 1 to 2 months.

[0049] The bacterial agent prepared according to the water hyacinth biological control strain J1 provided in the embodiment of the present invention is designed with a solar insect attractant lamp mechanism to improve its water hyacinth control efficiency. The solar insect attractant lamp mechanism comprises a solar electromagnetic panel, a battery, a waterproof black light, a fixing device and a connecting wire lamp. During the day, the solar electromagnetic panel stores electrical energy, and at night, the black light is turned on. It is used to lure insects in the surrounding environment to gnaw on the leaves and stems of water hyacinth, which can cause large-scale trauma to water hyacinth plants, significantly improve the infection efficiency of the control bacterial agent, promote the insect-borne transmission of the bacterial agent, and expand the scope of water hyacinth control.

[0050] The scope of use of the bacterial agent prepared according to the water hyacinth biological control strain J1 provided in the embodiment of the present invention includes water hyacinth flooded areas in flowing water bodies and static rivers and lakes.

[0051] Example 1: Isolation and purification of water hyacinth biological control strain J1.

[0052] Water hyacinths with different diseases were collected in a specific water hyacinth growth area for microbial culture, and the microorganisms were cultured using a potato glucose liquid medium to obtain and preserve the pathogenic strains of water hyacinth. By investigating the disease incidence of water hyacinths in a specific area, the symptoms of stem and leaf lesions and rots that exist under natural conditions were obtained through screening. This disease can effectively control the reproduction of water hyacinths. Plants with stem and leaf lesions and rots were brought back to the laboratory for pathogen isolation and culture, and the water hyacinth biological control strain J1 (see Figure 1 ).

[0053] Example 2: Testing the activity of Cercospora rodmanii J1 strain at different pH, carbon source and nitrogen source: (test temperature: 28 degrees Celsius).

[0054] Viability of strains at different pH values: Potato dextrose agar solid culture media with pH values ​​of 4.0, 5.0, 6.0, 7.0, and 8.0 were prepared by adjusting the ratio of disodium hydrogen phosphate solution and citric acid solution. Cercospora rodmanni J1 was inoculated in the culture media with different pH values. The diameter of the unique single colony was read every two days, and the data was recorded and plotted (see Figure 2(a)).

[0055] Viability of strains with different carbon and nitrogen sources: By setting up "sucrose + sodium nitrate", "sucrose + ammonium chloride", "sucrose + urea", "sucrose + glycine", "sodium nitrate + glucose" and "sodium nitrate + starch" control groups, the corresponding culture medium was prepared, and Cercospora rodmanni J1 was inoculated in the prepared culture medium. The diameter of the unique single colony was read every two days, and the data was recorded. The growth curves of Cercospora rodmanni J1 strains with different pH, carbon sources and nitrogen sources were plotted (see Figure 2 (b) and Figure 2 (c)). The culture medium formula involved in this experiment is shown in Table 1.

[0056] Table 1 Culture medium formula

[0057]

[0058]

[0059] Example 3: Adding 0.3% glycine to the Cercospora rodmanni J1 inoculant can effectively increase the probability of water hyacinth disease.

[0060] It was found in Example 2 that the main strains in the bacterial agent can show strong activity against glycine. Experimental group: In a specific water hyacinth growth area, 0.3% glycine Rodmanni Cercospora J1 bacterial agent was additionally added during spraying, and the water hyacinth infection was observed every three days. Control group: In a specific water hyacinth growth area, only the same amount of Rodmanni Cercospora J1 bacterial agent was sprayed, and the water hyacinth infection was observed every three days. Through statistical analysis of the incidence, it was found that the experimental group to which 0.3% glycine Rodmanni Cercospora J1 bacterial agent was added during spraying increased the incidence by about 20% compared with the control group.

[0061] Example 4: A solar-powered insect-attracting lamp mechanism is provided to promote transmission through insect vectors and expand the scope of water hyacinth prevention and control.

[0062] Experimental group: Insect traps were placed in specific water hyacinth growth areas to attract insect natural enemies to gather in the water hyacinth growth areas. There are many insects that feed on water hyacinths, and chewing on water hyacinths causes certain injuries to water hyacinths; on this basis, Cercospora Rodmanni J1 was sprayed, and the water hyacinth infection was observed every three days. Control group: Insect traps were not used to attract insects, and gauze was used to cover the water hyacinths in specific areas to reduce the impact of external insects on water hyacinths; the same amount of Cercospora Rodmanni J1 was sprayed, and the water hyacinth infection was observed every three days. By statistically analyzing the incidence of the disease, it was found that the experimental group that induced insects to chew on water hyacinths and then sprayed Cercospora Rodmanni J1 increased the incidence of the disease by about 135% compared with the control group. Among them, the solar insect trap mechanism and the insect media principle of the agent are as follows: Figure 3 shown.

[0063] 2. Application Examples: In order to prove the creativity and technical value of the technical solution of the present invention, this section provides application examples of the technical solution of the claims on specific products or related technologies.

[0064] The technical solution of the present invention has been successfully applied in the treatment of water hyacinth in Linwang Creek, Hui'an County, Quanzhou City, and has formed a demonstration. From January to March of that year, before the water hyacinth had a large-scale outbreak in the spring, the key accumulation areas of water hyacinth in Linwang Creek were surveyed by drone aerial photography, and water hyacinth biological control agents were sprayed in these key accumulation areas of water hyacinth for artificial intervention. The bacterial agent formula uses water hyacinth biological control strain J1 as the main ingredient, and the amount of water hyacinth added to the water hyacinth is> 10 10 CFU / mu of bacteria. The emulsifier content of the bacterial agent is 5-10wt%, the glycine addition amount is 3-5g / L, the dimethyl tetrachloride sodium is 20-50g / mu, and the pH is adjusted to 4-6. The outbreak of water hyacinth in the Linwangxi Demonstration River was effectively controlled, and the problem of water hyacinth flooding was solved. The outbreak period of water hyacinth flooding is from May to August. By spraying water hyacinth biological control agents in advance during the outbreak period and conducting artificial intervention, the outbreak of water hyacinth can be effectively controlled or prevented, achieving the purpose of controlling the growth and group spread of water hyacinth.

[0065] 3. Evidence of the effects of the embodiments. The embodiments of the present invention have achieved some positive effects during the development or use process, and indeed have great advantages over the prior art. The following content is described in conjunction with the data, charts, etc. of the test process.

[0066] Biological control by screening specialized plant pathogenic microorganisms is an emerging technology in many countries where water hyacinth is rampant. By competing with the water hyacinth host for nutrients and secreting toxins, the tissues and cells of water hyacinth are destroyed, and the growth and reproduction of water hyacinth are further controlled. Technical advantages: quick effect, good effect, low cost, little damage to the environment, easy to use, strong professionalism, suitable for the treatment of large water areas. In practical applications, according to the structure of water hyacinth and the environment in which it grows, the surrounding insect natural enemies such as mites, spider mites, moths, and weevils are induced by arranging solar insect traps to control the growth of water hyacinth leaves, and then a large amount of water hyacinth biological control agents are sprayed, or biological control and chemical control are combined. The application effect is more obvious, so as to achieve the goal of controlling the flooding and spread of water hyacinth. Compared with pure manual or mechanical salvage, the comprehensive management of water hyacinth by this technology can greatly save costs in manpower and economic investment, and can save more than 50% of economic costs on average each year. It is also less harmful to the environment and is sustainable.

[0067] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with the technical field within the technical scope disclosed by the present invention and within the spirit and principle of the present invention should be covered by the protection scope of the present invention.

Claims

1. An application of a water hyacinth biological control agent in inhibiting the growth and reproduction of water hyacinth, as well as in water hyacinth biological control and water ecological management, characterized in that: The bacterial agent is mainly composed of water hyacinth biological control strain J1, and the amount of the bacterial agent added to the water hyacinth is>10 10 CFU / mu of bacteria; the emulsifier content added in the bacterial agent is 5-10wt%, the glycine addition amount is 3-5g / L, the pH is adjusted to 4-6, and the applicable temperature is 15-40°C; the water hyacinth biological control strain J1 is Cercospora rodmanii J1, and its preservation number is GDMCC No: 62031.

2. The use according to claim 1, characterized in that The method for using the microbial agent is atomization spraying, and the application cycle is once every 1 to 2 months.

3. The use according to claim 1, characterized in that The use process of the bacterial agent also includes designing a solar insect attractant lamp mechanism.

4. The use according to claim 3, characterized in that The solar insect attractant lamp mechanism comprises a solar electromagnetic panel, a storage battery, a waterproof black light, a fixing device and a connecting wire light; the solar electromagnetic panel stores electric energy during the day and discharges it at night, and the black light is turned on to attract insects in the surrounding environment to gnaw on the leaves and stems of water hyacinth.

5. The use according to claim 1, characterized in that The application scope of the bacterial agent includes water hyacinth flooded areas in flowing water bodies and static rivers and lakes.

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