A signal-to-noise ratio improving device for fluorescent seed sorting

Abstract

The present invention provides a signal-to-noise ratio (SNR) enhancement device for fluorescent seed sorting, comprising a scattered light elimination unit and a transmitted light elimination unit. The scattered light elimination unit eliminates scattered stray light generated by the excitation light used to excite the fluorescence of the seeds to be sorted, while the transmitted light elimination unit eliminates transmitted stray light generated by the excitation light. By providing the scattered light elimination unit, the transmitted light elimination unit, an imaging background plate, a slit element, and a light box with a stray light elimination inner wall, the present invention eliminates stray light or enhances fluorescence contrast, thereby improving the fluorescence signal-to-noise ratio and thereby enhancing the accuracy of fluorescent seed sorting.

Description

Technical Field

[0001] The present invention relates to the field of seed sorting, and in particular to a signal-to-noise ratio improving device for fluorescent seed sorting. Background Art

[0002] Existing color sorters do not have high requirements for stray light within the system, so there is no need to design a complex signal-to-noise ratio improvement device. The third-generation hybrid rice has very high requirements for fluorescence detection sorters, and a new signal-to-noise ratio improvement device needs to be designed to improve the signal-to-noise ratio of the imaging system and increase the detection rate of fluorescent seeds. Summary of the Invention

[0003] In order to solve the above problems, the present invention provides a signal-to-noise ratio improving device for fluorescent seed sorting.

[0004] To achieve the above objectives, the present invention adopts the following specific technical solutions:

[0005] A signal-to-noise ratio improving device for fluorescent seed sorting includes: a scattered light elimination unit and a transmitted light elimination unit; the scattered light elimination unit eliminates scattered stray light generated by the excitation light used to excite the fluorescence of the seeds to be sorted when irradiating the seeds to be sorted, and the transmitted light elimination unit eliminates transmitted stray light generated by the excitation light when irradiating the seeds to be sorted.

[0006] Preferably, the scattered light elimination unit includes: a polarizer group and a long-pass filter; the polarizer group includes a light source polarizer and a camera polarizer with perpendicular polarization directions, the light source polarizer is placed at the light outlet of the light source for providing excitation light, and the camera polarizer is placed at the light entrance of the camera for receiving fluorescence; the long-pass filter is placed between the camera and the camera polarizer to separate fluorescence and scattered stray light.

[0007] Preferably, the transmitted light elimination unit is a stray light elimination plate group, which includes two stray light elimination plates. The stray light elimination surfaces of the two stray light elimination plates are arranged opposite to each other and are placed on the side of the seeds to be sorted away from the light source, and the transmitted stray light is incident on the area between the two stray light elimination plates.

[0008] Preferably, an imaging background plate for improving fluorescence contrast is also included. The imaging background plate is placed on the side of the seeds to be sorted away from the camera, and its working surface is treated to eliminate stray light. The working surface is perpendicular to the main optical axis of the camera.

[0009] Preferably, a slit element is further included, and the slit element is placed on the side of the light source polarizer away from the light source and can move perpendicular to the excitation light.

[0010] Preferably, a light box is further included, and the scattered light elimination unit, the transmitted light elimination unit, the imaging background plate and the slit element are all placed in the inner space of the light box; the inner wall of the light box is treated to eliminate stray light.

[0011] Preferably, the light source is a laser light source.

[0012] The present invention can achieve the following technical effects:

[0013] By setting up a scattered light elimination unit, a transmitted light elimination unit, an imaging background plate, a slit element and a light box with an inner wall that eliminates stray light, stray light is eliminated or fluorescence contrast is improved, thereby improving the fluorescence signal-to-noise ratio and thus improving the sorting accuracy of fluorescent seeds. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a structural diagram of a device for improving a signal-to-noise ratio according to an embodiment of the present invention;

[0015] Figure 2 Schematic diagram of the working principle of the signal-to-noise ratio improving device according to an embodiment of the present invention.

[0016] The reference numerals include: scattered light elimination unit 1, transmitted light elimination unit 2, imaging background plate 3, slit element 4, light box 5, light source 6, camera 7, excitation light 8, fluorescence 9, scattered stray light 10, transmitted stray light 11, light source polarizer 101, camera polarizer 102, long-pass filter 103, first stray light elimination plate 201, and second stray light elimination plate 201. DETAILED DESCRIPTION

[0017] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and do not constitute a limitation of the present invention.

[0018] like Figure 1 、 2 As shown, a signal-to-noise ratio improving device for fluorescent seed sorting includes: a scattered light elimination unit 1 and a transmitted light elimination unit 2; the scattered light elimination unit 1 eliminates scattered stray light 10 generated by the excitation light 8 used to excite the fluorescence 9 of the seeds to be sorted irradiating the seeds to be sorted, and the transmitted light elimination unit 2 eliminates transmitted stray light 11 generated by the excitation light 8 irradiating the seeds to be sorted; by eliminating the scattered stray light 10 and the transmitted stray light 11, the signal-to-noise ratio of the fluorescence 9 is improved, the recognition rate of weak fluorescence is improved, and the sorting accuracy of fluorescent seeds is improved.

[0019] In one embodiment of the present invention, a scattered light elimination unit 1 includes: a polarizer set and a long-pass filter 103. The polarizer set includes a light source polarizer 101 and a camera polarizer 102 with perpendicular polarization directions. The light source polarizer 101 is placed at the light outlet of a light source 6 for providing excitation light 8, and the camera polarizer 102 is placed at the light entrance of a camera 7 for receiving fluorescence 9. The polarization directions of the light source polarizer 101 and the camera polarizer 102 are perpendicular. That is, the vibration direction of the scattered stray light 10 formed by the reflection of the excitation light 8 is perpendicular to the camera polarizer 102, thereby significantly reducing the scattered stray light 10 entering the camera 7.

[0020] A long-pass filter 103 is placed between the camera 7 and the camera polarizer 102 to separate the fluorescence 9 from scattered stray light 10. The wavelength of the scattered stray light 10 is the same as the wavelength of the excitation light 8, which is the wavelength of the light emitted by the light source 6. By selecting a light source 6 whose emitted light wavelength differs significantly from that of the fluorescence 9, wavelength filtering can be performed through the long-pass filter 103, further reducing the scattered stray light 10 entering the camera 7. For example, in this embodiment, the wavelength of the emitted light is 532 nm, and the wavelength of the fluorescence 9 is 583 nm.

[0021] In one embodiment of the present invention, the transmitted light elimination unit 2 is a stray light elimination plate group, which includes a first stray light elimination plate 201 and a second stray light elimination plate 202. The stray light elimination surfaces of the first stray light elimination plate 201 and the second stray light elimination plate 202 are arranged opposite to each other and are placed on the side of the seeds to be sorted away from the light source 6. The transmitted stray light 11 is incident on the area between the first stray light elimination plate 201 and the second stray light elimination plate 202. The stray light elimination surface has a zigzag structure. The transmitted stray light 11 is reflected multiple times between the two stray light elimination plates and is absorbed and eliminated by the two stray light elimination plates during the propagation process.

[0022] In one embodiment of the present invention, an imaging background plate 3 for improving the contrast of fluorescence 9 is also included. The imaging background plate 3 is placed on the side of the seeds to be sorted away from the camera 7, and its working surface is treated to eliminate stray light. The working surface is perpendicular to the main optical axis of the camera 7. The setting of the imaging background plate 3 improves the contrast of the imaging system, and the fluorescence 9 excited on the back of the seeds to be sorted enters the camera 7 through reflection from the imaging background plate 3, thereby improving the energy of the fluorescence 9 and making it easier to identify.

[0023] In one embodiment of the present invention, a slit element 4 is further included. The slit element 4 is placed on the side of the light source polarizer 101 away from the light source 6 and can move perpendicular to the excitation light 8. The slit element 4 reduces the beam size of the excitation light 8, that is, reduces the reflection area of ​​the excitation light 8. The slit element 4 is moved to change the optical position of the excitation light 8, change the propagation direction of the scattered stray light 10, and reduce the stray light entering the camera 7.

[0024] In one embodiment of the present invention, a light box 5 is further included, and the scattered light elimination unit 1, the transmitted light elimination unit 2, the imaging background plate 3 and the slit element 4 are all placed in the internal space of the light box 5; the inner wall of the light box 5 is treated to eliminate stray light, and the inner wall after the stray light elimination treatment can absorb stray light transmitted to the inner wall and shield external stray light from entering the device.

[0025] In one embodiment of the present invention, the light source 6 is a laser light source. The laser light source has the characteristics of fixed wavelength and high energy concentration, which reduces the generation of stray light.

[0026] In the description of this specification, the reference terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0027] Although the embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art may make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present invention.

[0028] The above specific embodiments of the present invention do not constitute a limitation on the scope of protection of the present invention. Any other corresponding changes and modifications made based on the technical concept of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A signal-to-noise ratio improving device for fluorescent seed sorting, characterized in that: include: A scattered light elimination unit and a transmitted light elimination unit; the scattered light elimination unit eliminates scattered stray light generated by the excitation light used to excite the fluorescence of the seeds to be sorted irradiating the seeds to be sorted, and the transmitted light elimination unit eliminates transmitted stray light generated by the excitation light irradiating the seeds to be sorted; the transmitted light elimination unit is a stray light elimination plate group, and the stray light elimination plate group includes two stray light elimination plates, the stray light elimination surfaces of the two stray light elimination plates are arranged opposite to each other, and are placed on the side of the seeds to be sorted away from the light source, and the transmitted stray light is incident on the area between the two stray light elimination plates.

2. The signal-to-noise ratio improving device for fluorescent seed sorting according to claim 1, characterized in that: The scattered light elimination unit includes: a polarizer group and a long-pass filter; the polarizer group includes a light source polarizer and a camera polarizer with perpendicular polarization directions, the light source polarizer is placed at the light outlet of the light source for providing the excitation light, and the camera polarizer is placed at the light entrance of the camera for receiving fluorescence; the long-pass filter is placed between the camera and the camera polarizer to separate fluorescence and scattered stray light.

3. The signal-to-noise ratio improving device for fluorescent seed sorting according to claim 2, characterized in that: It also includes an imaging background plate for improving fluorescence contrast, which is placed on the side of the seeds to be sorted away from the camera, and its working surface is treated to eliminate stray light, and the working surface is perpendicular to the main optical axis of the camera.

4. The signal-to-noise ratio improving device for fluorescent seed sorting according to claim 3, characterized in that: It also includes a slit element, which is placed on the side of the light source polarizer away from the light source and can move perpendicular to the excitation light.

5. The signal-to-noise ratio improving device for fluorescent seed sorting according to claim 4, characterized in that: It also includes a light box, wherein the scattered light elimination unit, the transmitted light elimination unit, the imaging background plate and the slit element are all placed in the inner space of the light box; the inner wall of the light box is treated to eliminate stray light.

6. The signal-to-noise ratio improving device for fluorescent seed sorting according to claim 2, characterized in that: The light source is a laser light source.

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