Composition for treating mosquito bites, preparation method and pharmaceutical use thereof

Abstract

The present invention provides a composition for treating mosquito bites, which is made of the following raw materials: patchouli, atractylodes, cinnamon, schizonepeta, elsholtzia, wormwood, clove, prickly ash, mint and natural borneol. The composition for treating mosquito bites of the present invention has a good therapeutic effect on mosquito bites, can relieve itching and pain, reduce inflammation, reduce swelling, prevent infection, prevent the spread of diseases, and has a mild formula and does not irritate the skin. Moreover, the composition for treating mosquito bites of the present invention is a traditional Chinese medicine component, and compared with chemical drugs, it not only has a good effect of treating mosquito bites, but also avoids the side effects produced by chemical drugs.

Description

Technical Field

[0001] The invention belongs to the field of medicine, and particularly relates to a composition for treating mosquito bites, a preparation method thereof and pharmaceutical use thereof. Background Art

[0002] Being bitten by mosquitoes is a very common phenomenon. Generally, mosquito bites may cause redness, swelling, inflammation, itching, pain and other symptoms. In addition, mosquito bites can also spread some diseases. Drugs can be used to relieve itching, pain, inflammation, swelling, and prevent infection and the spread of diseases.

[0003] At present, the topical drugs commonly used for treating mosquito bites are mostly chemical drugs, which have certain side effects. Moreover, most drugs for treating mosquito bites contain alcohol, which can irritate the skin and are not suitable for people with skin allergies. Therefore, it is necessary to develop a drug for treating mosquito bites that can relieve itching and pain, reduce inflammation, reduce swelling, prevent infection and prevent the spread of diseases, and does not irritate the skin and has few side effects. Summary of the invention

[0004] In view of the deficiencies of the prior art, the object of the present invention is to provide a composition for treating mosquito bites.

[0005] In order to achieve the above object, the composition for treating mosquito bites of the present invention is made of the following raw materials:

[0006] Patchouli 4-14 parts by weight, Atractylodes 9-28 parts by weight, Cinnamon 0.5-4 parts by weight, Nepeta 1-5 parts by weight, Elsholtzia 5-18 parts by weight, Artemisia argyi 11-33 parts by weight, Cloves 0.1-3 parts by weight, Zanthoxylum bungeanum 0.5-2 parts by weight and Mint 2-10 parts by weight.

[0007] Preferably, in the composition for treating mosquito bites according to the present invention, the raw materials further include 0.01-0.2 parts by weight of natural borneol.

[0008] Patchouli is aromatic and can remove dampness, dispel heat and relieve symptoms, Atractylodes lancea can dry out dampness and strengthen the spleen, dispel wind and dissipate cold, and Zanthoxylum bungeanum can kill insects and relieve itching. The above three main herbs are used together to kill insects, ward off filth and relieve itching. Elsholtzia detoxifies and relieves itching, Artemisia argyi removes dampness and relieves itching and stops bleeding, Clove kills insects, wards off evil and removes evil, Cinnamon kills insects, and Nepeta stop bleeding and seals toxic swelling. The above five auxiliary herbs can not only help the main herbs to kill insects and relieve itching, but also detoxify, stop bleeding and reduce swelling. Peppermint is pungent and cool to reduce swelling, stop bleeding and treat insect wounds, and natural borneol clears heat and relieves pain. The two auxiliary herbs are used together to enhance the effects of reducing swelling, stopping bleeding and relieving pain, while reconciling the warm and dry nature of the main and auxiliary herbs.

[0009] Further preferably, the composition for treating mosquito bites according to the present invention is made from the following raw materials:

[0010] 8-10 parts by weight of patchouli, 18-20 parts by weight of atractylodes, 0.6-2.8 parts by weight of cinnamon, 1.2-3.8 parts by weight of schizonepeta, 10-12 parts by weight of elsholtzia, 20-24 parts by weight of wormwood, 0.4-2.5 parts by weight of cloves, 0.5-1.5 parts by weight of peppermint, 4-8 parts by weight of mint, and 0.01-0.15 parts by weight of natural borneol.

[0011] Preferably, the composition for treating mosquito bites of the present invention further comprises a pharmaceutical excipient.

[0012] Preferably, the composition for treating mosquito bites of the present invention further comprises 0.2-64 parts by weight of pharmaceutical excipients, preferably 8-46 parts by weight of pharmaceutical excipients, and the pharmaceutical excipients can be selected from one or more of medium-chain triglycerides, castor oil, hemp seed oil, olive oil, soybean oil, corn oil, peanut oil, coconut oil, tea seed oil, grape seed oil, glycerin, beeswax, stearic acid, Tween-80, lanolin, carbomer 980, hydroxypropyl-β-cyclodextrin, palm wax, preferably medium-chain triglycerides.

[0013] Preferably, the composition for treating mosquito bites of the present invention may be in the form of volatile oil. Therefore, the present invention also provides volatile oil for treating mosquito bites.

[0014] Another object of the present invention is to provide a method for preparing a composition for treating mosquito bites, which can be prepared by a subcritical water extraction method, comprising the following steps:

[0015] The patchouli is crushed, and the crushed patchouli is extracted in water for 30 to 40 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 140 to 160° C. to obtain patchouli volatile oil, wherein the water-to-material ratio is 15 to 20:1 (mL / g), and the extraction rate of the patchouli volatile oil is 0.8 to 1.1%, and the extraction pressure can be any pressure in the range of 5 to 10 MPa, for example, 5, 6, 7, 8, 9 or 10 MPa, and the extraction temperature can be any temperature in the range of 140 to 160° C., for example, 140, 145, 150, 155 or 160° C.,

[0016] The method comprises the steps of: crushing the atractylodes lancea, extracting the crushed atractylodes lancea in water for 50 to 60 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 150 to 170° C., and obtaining atractylodes lancea volatile oil, wherein the water-to-material ratio is 15 to 18:1 (mL / g), and the extraction rate of the atractylodes lancea volatile oil is 0.7 to 1.1%, and wherein the extraction pressure can be any pressure within the range of 5 to 10 MPa, for example, 5, 6, 7, 8, 9 or 10 MPa, and the extraction temperature can be any temperature within the range of 150 to 170° C., for example, 150, 155, 160, 165 or 170° C.,

[0017] The cinnamon is crushed, and the crushed cinnamon is extracted in water for 40 to 50 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 150 to 160° C. to obtain cinnamon volatile oil, wherein the water-to-material ratio is 13 to 15:1 (mL / g), the extraction rate of cinnamon volatile oil is 1.5 to 2.0%, and the extraction pressure can be any pressure in the range of 5 to 10 MPa, for example, 5, 6, 7, 8, 9 or 10 MPa, and the extraction temperature can be any temperature in the range of 150 to 160° C., for example, 150, 155 or 160° C.,

[0018] The schizonepeta is crushed, and the crushed schizonepeta is extracted in water for 30 to 40 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 130 to 140° C. to obtain schizonepeta volatile oil, wherein the water-to-material ratio is 15 to 20:1 (mL / g), and the extraction rate of schizonepeta volatile oil is 0.7 to 0.9%, and the extraction pressure can be any pressure in the range of 5 to 10 MPa, for example, 5, 6, 7, 8, 9 or 10 MPa, and the extraction temperature can be any temperature in the range of 130 to 140° C., for example, 130, 135 or 140° C.,

[0019] The herb is crushed, and the crushed herb is extracted in water for 30 to 40 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 140 to 160° C. to obtain a herb volatile oil, wherein the water-to-material ratio is 15 to 20:1 (mL / g), and the extraction rate of the herb volatile oil is 0.8 to 1.1%, and the extraction pressure can be any pressure in the range of 5 to 10 MPa, for example, 5, 6, 7, 8, 9 or 10 MPa, and the extraction temperature can be any temperature in the range of 140 to 160° C., for example, 140, 145, 150, 155 or 160° C.,

[0020] The wormwood leaf is crushed, and the crushed wormwood leaf is extracted in water for 30 to 40 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 135 to 145° C. to obtain wormwood leaf volatile oil, wherein the water-to-material ratio is 15 to 20:1 (mL / g), the extraction rate of the wormwood leaf volatile oil is 0.5 to 0.7%, and the extraction pressure can be any pressure in the range of 5 to 10 MPa, for example, 5, 6, 7, 8, 9 or 10 MPa, and the extraction temperature can be any temperature in the range of 135 to 145° C., for example, 135, 140 or 145° C.

[0021] The cloves are crushed, and the crushed cloves are extracted in water for 30 to 40 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 135 to 145° C. to obtain clove volatile oil, wherein the water-to-material ratio is 15 to 18:1 (mL / g), the extraction rate of the clove volatile oil is 16 to 19%, and the extraction pressure can be any pressure in the range of 5 to 10 MPa, for example, 5, 6, 7, 8, 9 or 10 MPa, and the extraction temperature can be any temperature in the range of 135 to 145° C., for example, 135, 140 or 145° C.

[0022] The peppercorns are crushed, and the crushed peppercorns are extracted in water for 30 to 40 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 135 to 150° C. to obtain peppercorn volatile oil, wherein the water-to-material ratio is 15 to 18:1 (mL / g), and the extraction rate of the peppercorn volatile oil is 4.0 to 5.0%, and the extraction pressure can be any pressure in the range of 5 to 10 MPa, for example, 5, 6, 7, 8, 9 or 10 MPa, and the extraction temperature can be any temperature in the range of 135 to 150° C., for example, 135, 140, 145 or 150° C.,

[0023] The mint is crushed, and the crushed mint is extracted in water for 30 to 40 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 135 to 145° C. to obtain mint volatile oil, wherein the water-to-material ratio is 15 to 20:1 (mL / g), the extraction rate of the mint volatile oil is 0.8 to 1.0%, and the extraction pressure can be any pressure in the range of 5 to 10 MPa, for example, 5, 6, 7, 8, 9 or 10 MPa, and the extraction temperature can be any temperature in the range of 135 to 145° C., for example, 135, 140 or 145° C.,

[0024] The volatile oils obtained from the above raw materials are mixed to obtain the product.

[0025] Preferably, the preparation method of the composition for treating mosquito bites of the present invention comprises the following steps:

[0026] The patchouli is crushed and extracted in water at an extraction pressure of 5 MPa and an extraction temperature of 150°C for 30 minutes to obtain patchouli volatile oil.

[0027] The Atractylodes lancea was crushed, and the crushed Atractylodes lancea was extracted in water for 60 minutes at an extraction pressure of 5 MPa and an extraction temperature of 160°C to obtain Atractylodes lancea volatile oil.

[0028] The cinnamon is crushed, and the crushed cinnamon is extracted in water at an extraction pressure of 5 MPa and an extraction temperature of 160° C. for 40 minutes to obtain cinnamon volatile oil.

[0029] The schizonepeta was crushed, and the crushed schizonepeta was extracted in water at an extraction pressure of 5 MPa and an extraction temperature of 140° C. for 30 minutes to obtain schizonepeta volatile oil.

[0030] The Elsholtzia ciliata was crushed, and the crushed Elsholtzia ciliata was extracted in water at an extraction pressure of 5 MPa and an extraction temperature of 150° C. for 30 min to obtain the Elsholtzia ciliata volatile oil.

[0031] The wormwood leaf was crushed, and the crushed wormwood leaf was extracted in water at an extraction pressure of 5 MPa and an extraction temperature of 135°C for 30 minutes to obtain wormwood leaf volatile oil.

[0032] The cloves were crushed and extracted in water at an extraction pressure of 5 MPa and an extraction temperature of 135°C for 30 minutes to obtain clove volatile oil.

[0033] The peppercorns were crushed and extracted in water at an extraction pressure of 5 MPa and an extraction temperature of 140°C for 40 minutes to obtain peppercorn volatile oil.

[0034] The mint was crushed and extracted in water at an extraction pressure of 5 MPa and an extraction temperature of 135°C for 30 minutes to obtain mint volatile oil.

[0035] The volatile oils obtained from the above raw materials are mixed to obtain the product.

[0036] Preferably, natural borneol is added to the mixture of volatile oils obtained from the above raw materials and stirred to completely dissolve.

[0037] Another object of the present invention is to provide a method for preparing a composition for treating mosquito bites, which can be prepared by a subcritical water extraction method, comprising the following steps:

[0038] Patchouli, atractylodes, cinnamon, schizonepeta, elsholtzia, wormwood, clove, prickly ash and mint are crushed and mixed, and the crushed raw material mixture is extracted in water for 30 to 60 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 130 to 180°C to obtain total volatile oil, wherein the water-to-material ratio of the raw material mixture to water is 15 to 20:1 (ml / g), and the extraction pressure can be any pressure in the range of 5 to 10 MPa, for example, 5, 6, 7, 8, 9 or 10 MPa, and the extraction temperature can be any temperature in the range of 130 to 180°C, for example, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175 or 180°C.

[0039] Preferably, natural borneol is added to the total volatile oil and stirred to completely dissolve.

[0040] Another object of the present invention is to provide use of the above composition in preparing a medicament for treating mosquito bites.

[0041] Preferably, the medicine for treating mosquito bites of the present invention can be in the form of various preparations such as sprays, aerosols, liniments, creams, ointments, ointments, gels, solutions, and spreads. Therefore, the present invention also provides various preparations such as sprays, aerosols, liniments, creams, ointments, ointments, gels, solutions, and spreads for treating mosquito bites.

[0042] Furthermore, the spray, aerosol, liniment, cream, ointment, creams, gels, solutions and smears for treating mosquito bites of the present invention can be obtained by conventional methods in the art. For example, the spray for treating mosquito bites of the present invention can be obtained by dispensing the above-mentioned composition of the present invention into a spray bottle; the aerosol for treating mosquito bites of the present invention can be obtained by dispensing the above-mentioned composition of the present invention into an aerosol aluminum can, pressing the cap, and filling with nitrogen to make the internal pressure within the range of 0.8 to 1.0 MPa; the liniment for treating mosquito bites of the present invention can be obtained by dispensing the above-mentioned composition of the present invention into a roller bottle.

[0043] In summary, the composition for treating mosquito bites of the present invention, the volatile oil or the pharmaceutical preparation prepared therefrom has a good therapeutic effect on mosquito bites, can relieve itching and pain, reduce inflammation, reduce swelling, prevent infection, prevent the spread of diseases, and the formula is mild and does not irritate the skin. Moreover, the composition for treating mosquito bites of the present invention, the volatile oil or the pharmaceutical preparation prepared therefrom is a traditional Chinese medicine component, and compared with chemical drugs, it not only has a good effect of treating mosquito bites, but also avoids the side effects produced by chemical drugs. Specific implementation plan

[0044] In order to better understand the content of the present invention, it is further described below in conjunction with embodiments, but the content of the present invention is not limited to these embodiments.

[0045] The sources of various raw materials and reagents used in the examples of the present invention are as follows:

[0046] Artemisia argyi slices, batch number: 200301, Kunming authentic Chinese medicine slices factory

[0047] Mint slices, batch number: 200301, Kunming Authentic Chinese Medicine Slices Factory

[0048] Atractylodes macrocephala slices, batch number: 200301, Kunming Authentic Chinese Medicine Slices Factory

[0049] Clove slices, batch number: 200201, Kunming Authentic Chinese Medicine Slices Factory

[0050] Patchouli slices, batch number: 200201, Kunming Authentic Chinese Medicine Slices Factory

[0051] Elsholtzia slices, batch number: 1909001, Lingnan Chinese Medicine Slices Co., Ltd.

[0052] Natural borneol, batch number: 202002003, Guangdong Huaqingyuan Biotechnology Co., Ltd.

[0053] Cinnamon slices, batch number: DD20160201, Yunnan Baiyao Group Chinese Medicine Resources Division

[0054] Nepeta decoction pieces, batch number: YP20200401, Yunnan Baiyao Group Chinese Medicine Resources Division

[0055] You can buy Sichuan pepper medicinal materials at the market.

[0056] Example

[0057] Example 1 Preparation of a composition for treating mosquito bites

[0058] 1. Volatile Oil Extraction

[0059] 1.1 Extraction of Patchouli Volatile Oil

[0060] Take patchouli medicinal materials, crush them, pass them through a 20-mesh sieve, take 400 g of patchouli medicinal material powder, place it in a subcritical water extractor, add 6000 mL of water, extract for 30 minutes at an extraction pressure of 5 MPa and an extraction temperature of 150° C., separate, dehydrate, and obtain patchouli volatile oil with an extraction rate of 0.95%.

[0061] 1.2 Extraction of volatile oil from Atractylodes lancea

[0062] Take the Atractylodes lancea medicinal material, crush it, pass it through a 20-mesh sieve, take 900 g of the Atractylodes lancea medicinal material powder, place it in a subcritical water extractor, add 13500 mL of water, extract for 60 minutes at an extraction pressure of 5 MPa and an extraction temperature of 160°C, separate, dehydrate, and obtain Atractylodes lancea volatile oil, with an extraction rate of 1.01%.

[0063] 1.3 Extraction of cinnamon volatile oil

[0064] Take cinnamon medicinal materials, crush them, pass them through a 20-mesh sieve, take 60g of cinnamon medicinal material powder, put it in a subcritical water extractor, add 900mL of water, extract for 50min at an extraction pressure of 5Mpa and an extraction temperature of 155°C, separate and dehydrate to obtain cinnamon volatile oil, with an extraction rate of 1.65%.

[0065] 1.4 Extraction of Nepeta volatile oil

[0066] Take the medicinal material of Nepeta, crush it, pass it through a 20-mesh sieve, take 120g of the medicinal material powder of Nepeta, place it in a subcritical water extractor, add 2100mL of water, extract for 30min at an extraction pressure of 5Mpa and an extraction temperature of 135°C, separate, dehydrate, and obtain Nepeta volatile oil, with an extraction rate of 0.87%.

[0067] 1.5 Extraction of volatile oil from Elsholtzia ciliata

[0068] Take the Elsholtzia medicinal material, crush it, pass it through a 20-mesh sieve, take 500 g of the Elsholtzia medicinal material powder, put it in a subcritical water extractor, add 7500 mL of water, extract for 30 minutes at an extraction pressure of 5 MPa and an extraction temperature of 150° C., separate, dehydrate, and obtain the Elsholtzia volatile oil, with an extraction rate of 0.95%.

[0069] 1.6 Extraction of volatile oil from Artemisia argyi

[0070] Take the wormwood leaf medicinal material, crush it, pass it through a 20-mesh sieve, take 1100g of the wormwood leaf medicinal material powder, place it in a subcritical water extractor, add 16500mL of water, extract for 30min at an extraction pressure of 5Mpa and an extraction temperature of 135°C, separate, dehydrate, and obtain wormwood leaf volatile oil with an extraction rate of 0.65%.

[0071] 1.7 Clove volatile oil extraction

[0072] Take clove medicinal materials, crush them, pass them through a 20-mesh sieve, take 10 g of clove medicinal material powder, place it in a subcritical water extractor, add 180 mL of water, extract for 30 minutes at an extraction pressure of 5 MPa and an extraction temperature of 135°C, separate, dehydrate, and obtain clove volatile oil with an extraction rate of 17.5%.

[0073] 1.8 Extraction of volatile oil from Zanthoxylum bungeanum

[0074] Take the Zanthoxylum bungeanum medicinal material, crush it, pass it through a 20-mesh sieve, take 50g of the Zanthoxylum bungeanum medicinal material powder, place it in a subcritical water extractor, add 750mL of water, extract for 30min at an extraction pressure of 5Mpa and an extraction temperature of 140°C, separate, dehydrate, and obtain Zanthoxylum bungeanum volatile oil, with an extraction rate of 4.12%.

[0075] 1.9 Extraction of peppermint essential oil

[0076] Take mint medicinal materials, crush them, pass them through a 20-mesh sieve, take 200 g of mint medicinal material powder, place it in a subcritical water extractor, add 3000 mL of water, extract for 30 minutes at an extraction pressure of 5 MPa and an extraction temperature of 135° C., separate, dehydrate, and obtain mint volatile oil with an extraction rate of 0.98%.

[0077] 2. Preparation of main drug mixture

[0078] The volatile oils extracted in steps 1.1 to 1.9 are mixed evenly, 4 g of natural borneol is added, and stirred to dissolve, thereby obtaining a main drug mixture, namely, the composition for treating mosquito bites of the present invention.

[0079] Example 2 Preparation of a composition for treating mosquito bites

[0080] 1. Volatile Oil Extraction

[0081] 1.1 Extraction of Patchouli Volatile Oil

[0082] Take patchouli medicinal materials, crush them, pass them through a 20-mesh sieve, take 800 g of patchouli medicinal material powder, place it in a subcritical water extractor, add 16000 mL of water, extract for 30 minutes at an extraction pressure of 5 MPa and an extraction temperature of 150° C., separate and dehydrate to obtain patchouli volatile oil, with an extraction rate of 0.98%.

[0083] 1.2 Extraction of volatile oil from Atractylodes lancea

[0084] Take the Atractylodes lancea medicinal material, crush it, pass it through a 20-mesh sieve, take 1800 g of the Atractylodes lancea medicinal material powder, place it in a subcritical water extractor, add 32400 mL of water, extract for 60 minutes at an extraction pressure of 5 MPa and an extraction temperature of 160° C., separate, dehydrate, and obtain Atractylodes lancea volatile oil, with an extraction rate of 0.95%.

[0085] 1.3 Extraction of cinnamon volatile oil

[0086] Take cinnamon medicinal materials, crush them, pass them through a 20-mesh sieve, take 150g of cinnamon medicinal material powder, place it in a subcritical water extractor, add 2250mL of water, extract for 50min at an extraction pressure of 10Mpa and an extraction temperature of 160°C, separate and dehydrate to obtain cinnamon volatile oil, with an extraction rate of 1.65%.

[0087] 1.4 Extraction of Nepeta volatile oil

[0088] Take the medicinal material of Nepeta, crush it, pass it through a 20-mesh sieve, take 300 g of the medicinal material powder of Nepeta, place it in a subcritical water extractor, add 6000 mL of water, extract for 40 minutes at an extraction pressure of 10 MPa and an extraction temperature of 140°C, separate, dehydrate, and obtain Nepeta volatile oil, with an extraction rate of 0.85%.

[0089] 1.5 Extraction of volatile oil from Elsholtzia ciliata

[0090] Take the Elsholtzia medicinal material, crush it, pass it through a 20-mesh sieve, take 1000 g of the Elsholtzia medicinal material powder, put it in a subcritical water extractor, add 20000 mL of water, extract for 30 minutes at an extraction pressure of 5 MPa and an extraction temperature of 150°C, separate, dehydrate, and obtain the Elsholtzia volatile oil, with an extraction rate of 0.90%.

[0091] 1.6 Extraction of volatile oil from Artemisia argyi

[0092] Take wormwood medicinal material, crush it, pass it through a 20-mesh sieve, take 2000g of wormwood medicinal material powder, place it in a subcritical water extractor, add 40000mL of water, extract for 30min at an extraction pressure of 5Mpa and an extraction temperature of 135°C, separate, dehydrate, and obtain wormwood volatile oil with an extraction rate of 0.62%.

[0093] 1.7 Clove volatile oil extraction

[0094] Take clove medicinal materials, crush them, pass them through a 20-mesh sieve, take 40 g of clove medicinal material powder, place it in a subcritical water extractor, add 720 mL of water, extract for 30 minutes at an extraction pressure of 5 MPa and an extraction temperature of 135°C, separate, dehydrate, and obtain clove volatile oil with an extraction rate of 16.8%.

[0095] 1.8 Extraction of volatile oil from Zanthoxylum bungeanum

[0096] Take the Zanthoxylum bungeanum medicinal material, crush it, pass it through a 20-mesh sieve, take 120 g of the Zanthoxylum bungeanum medicinal material powder, place it in a subcritical water extractor, add 1800 mL of water, extract for 40 minutes at an extraction pressure of 10 MPa and an extraction temperature of 150° C., separate, dehydrate, and obtain Zanthoxylum bungeanum volatile oil, with an extraction rate of 4.07%.

[0097] 1.9 Extraction of peppermint essential oil

[0098] Take mint medicinal materials, crush them, pass them through a 20-mesh sieve, take 400 g of mint medicinal material powder, put it in a subcritical water extractor, add 8000 mL of water, extract for 30 minutes at an extraction pressure of 5 MPa and an extraction temperature of 135° C., separate, dehydrate, and obtain mint volatile oil with an extraction rate of 0.95%.

[0099] 2. Preparation of main drug mixture

[0100] The volatile oils extracted in steps 1.1 to 1.9 are mixed evenly, 1 g of natural borneol is added, and stirred to dissolve, thereby obtaining a main drug mixture, namely, the composition for treating mosquito bites of the present invention.

[0101] Example 3 Preparation of a composition for treating mosquito bites

[0102] 1. Mixed volatile oil extraction

[0103] Take nine medicinal materials including patchouli, atractylodes, cinnamon, schizonepeta, Elsholtzia, wormwood, cloves, Zanthoxylum bungeanum and mint, grind them separately, pass them through a 20-mesh sieve, take 200g of patchouli powder, 450g of atractylodes powder, 40g of cinnamon powder, 75g of schizonepeta powder, 250g of Elsholtzia powder, 500g of wormwood powder, 10g of clove powder, 30g of Zanthoxylum bungeanum powder and 100g of mint powder respectively, put them in a subcritical water extractor, add 25000mL of water, extract for 30min at an extraction pressure of 5Mpa and an extraction temperature of 135°C, separate and dehydrate to obtain a mixed volatile oil.

[0104] 2. Preparation of main drug mixture

[0105] Add 1 g of natural borneol to the mixed volatile oil extracted in step 1, stir to dissolve, and obtain a main drug mixture, that is, the composition for treating mosquito bites of the present invention.

[0106] Example 4 Preparation of spray

[0107] Add 890 g of medium-chain triglycerides or olive oil to the main drug mixture prepared in Example 1, make up the volume to 1000 mL, stir to obtain a clear liquid, and fill it into a spray bottle, 10 mL / bottle.

[0108] Example 5 Preparation of Aerosol

[0109] Add 860 g of medium-chain triglyceride or soybean oil to the main drug mixture prepared in Example 2, make up the volume to 1000 mL, stir to obtain a clear liquid, fill it into an aerosol bottle, 10 mL / bottle, press the cap, and fill with nitrogen to make the internal pressure reach 0.8-1.0 MPa.

[0110] Example 6 Preparation of Liniment

[0111] Add 900 g of medium-chain triglycerides or corn oil to the main drug mixture prepared in Example 3, make up the volume to 1000 mL, stir to obtain a clear liquid, and put it into a roller bottle, 10 mL / bottle.

[0112] Example 7 Preparation of cream

[0113] Prepare the main drug mixture according to the method of Example 3, add 10g glycerol, 2g stearic acid, 5g medium-chain triglyceride or peanut oil, and 1g Tween-80, stir and dissolve in a 60°C water bath, then add 40g water, maintain a 60°C water bath, stir while adding, stir for 30min after adding water to completely emulsify, and cool to room temperature to obtain an ointment.

[0114] Example 8 Ointment Preparation

[0115] Prepare the main drug mixture according to the method of Example 3, add 200g of medium-chain triglyceride or coconut oil, 30g of glycerol, and 20g of beeswax, stir and dissolve in a 60°C water bath, stir for 30 minutes, and cool to room temperature to obtain an ointment.

[0116] Example 9 Preparation of Spray

[0117] Add 900 g of medium-chain triglycerides to the main drug mixture prepared in Example 1, make up the volume to 1000 mL, stir to obtain a clear liquid, and fill it into a spray bottle, 10 mL / bottle.

[0118] Example 10 Preparation of aerosol

[0119] Add 870 g of medium-chain triglyceride or castor oil to the main drug mixture prepared in Example 2, make up the volume to 1000 mL, stir to obtain a clear liquid, fill it into an aerosol bottle, 10 mL / bottle, press the cap, and fill with nitrogen to make the internal pressure reach 0.8-1.0 MPa.

[0120] Example 11 Preparation of Liniment

[0121] Add 910 g of medium-chain triglycerides or hemp seed oil to the main drug mixture prepared in Example 3, make up the volume to 1000 mL, stir to obtain a clear liquid, and put it into a roller bottle, 10 mL / bottle.

[0122] Example 12 Preparation of ointment

[0123] Prepare the main drug mixture according to the method of Example 3, take 800g of tea seed oil, add 50g of palm wax, heat and stir at 65°C to completely dissolve the palm wax, add the main drug mixture, stir evenly, and divide into packages to obtain an ointment.

[0124] Example 13 Preparation of gel

[0125] The main drug mixture was prepared according to the method of Example 3. 10 g of Carbomer 980 was added to 500 ml of water for dissolution and refrigerated overnight to fully swell to obtain Liquid A. 100 g of hydroxypropyl-β-cyclodextrin was dissolved in 500 ml of water and stirred at 50° C. The main drug mixture was added dropwise and stirred to completely dissolve to obtain Liquid B. Liquid A and Liquid B were mixed and triethanolamine was added to adjust the pH to 4.5 to 8.5 to obtain a gel.

[0126] Example 14 Preparation of solution

[0127] Prepare the main drug mixture according to the method of Example 3, add 500g of tea seed oil, stir evenly, and apply it to the affected area with a cotton swab or fingers when using.

[0128] Example 15 Preparation of the coating agent

[0129] Prepare the main drug mixture according to the method of Example 3, weigh 200g of grape seed oil, add 150g of lanolin and 100g of beeswax, stir in a 65°C water bath to completely dissolve the beeswax, add the main drug mixture, stir evenly, pour the mixed solution into the lip balm mold while hot, cool it, and obtain a stick-shaped spread. Different shapes of spreads can also be obtained by using molds of different shapes.

[0130] Experimental Section

[0131] The inventors have found that the composition of the present invention can be used to treat local itching, redness, swelling, inflammation, etc. caused by mosquito bites. The following provides experimental data to prove that the composition of the present invention can be used to treat local itching, redness, swelling, inflammation, etc. caused by mosquito bites. The guinea pig skin itching model induced by histamine phosphate, the mouse writhing experiment induced by glacial acetic acid, and the mouse ear swelling model induced by xylene were used to investigate the pharmacodynamics of the composition of the present invention for mosquito bites from the two aspects of antipruritic and anti-inflammatory swelling.

[0132] The composition for treating mosquito bites prepared in Example 2 of the present invention (hereinafter referred to as the composition of the present invention) was used for the following experiment.

[0133] 1. Histamine phosphate-induced pruritus experiment on guinea pig skin

[0134] 1.1 Experimental methods

[0135] 60 ordinary guinea pigs (purchased from Kunming Chushang Biotechnology Co., Ltd., experimental animal production license number: SCXK (Yunnan) K2018-0001), weighing 250-300 g, half male and half female, were randomly divided into 6 groups after conventional feeding for 7 days, with 10 in each group, namely, model control group, compound dexamethasone acetate cream (commercially available) group (50 mg / mouse), low-dose (19 mg / kg) group and high-dose (38 mg / kg) group of the composition of the present invention. The hair on the back of the right hind foot of each guinea pig was shaved. The next day, each test group was smeared on the back of the right foot of the guinea pig according to the corresponding dose, and the administration volume was 125 μL / kg for 3 consecutive days. The model group was given a corresponding volume of normal saline.

[0136] One hour after the last administration, use fine sandpaper to gently scratch the shaved area of ​​the right hind foot of the guinea pig to make it red but not bleed. The area is 1 cm 2 The drug was administered once more. After 10 minutes, the test substance was removed with a cotton swab dipped in physiological saline, and 50 μL / guinea pig of 0.01% histamine phosphate was dripped on the wound surface. Thereafter, the concentration was increased by 0.02%, 0.03%, 0.04%... every 3 minutes, and 50 μL / guinea pig each time, until the guinea pig turned back to lick the hind paw on that side. The antipruritic effect was evaluated using the itch threshold of guinea pigs induced by histamine phosphate (itch threshold, i.e., the total amount of histamine phosphate administered when the guinea pig turned back to lick the wound on the hind paw) as an indicator.

[0137] 1.2 Experimental Results

[0138] Table 1. Effect of the composition of the present invention on the itch threshold of guinea pigs induced by histamine phosphate

[0139]

[0140] Note: Compared with the model group, b P<0.01, c P<0.001.

[0141] The experimental results in Table 1 show that compared with the model group, the 50 mg / kg dose group of the compound dexamethasone acetate cream and the 19 and 38 mg / kg dose groups of the composition samples of the present invention can significantly increase the itch threshold of guinea pigs (P<0.01 or P<0.001).

[0142] 2. Glacial acetic acid-induced writhing experiment in mice

[0143] 2.1 Experimental methods

[0144] 96 SPF KM mice (purchased from Beijing Huafukang Biotechnology Co., Ltd., experimental animal production license number: SCXK (Beijing) 2019-0008), weighing 20±2g, all male, were randomly divided into 8 groups after conventional feeding for 3 days, with 12 mice in each group, namely, blank group, positive control dexamethasone group, low, medium and high dose (11.0 mg / kg, 27.5 mg / kg and 55 mg / kg) groups of the composition of the present invention. The animals were smeared on the abdomen and administered, 30 μL / mouse, and the blank group was given an equal volume of pure water, once / d, for 3 consecutive days.

[0145] One hour after the last administration, 0.6% glacial acetic acid was injected intraperitoneally at a dosage volume of 10 mL / kg. Ten minutes later, the number of writhing reactions of the mice within 15 minutes was observed (based on the appearance of abdominal concavity, extension of trunk and hind limbs, and lifting of buttocks in the mice), and the analgesic rate was calculated: analgesic rate (%) = (average number of writhing times in the model group - average number of writhing times in the drug administration group) / average number of writhing times in the model group × 100%.

[0146] 2.2 Experimental Results

[0147] Table 2. Effect of the composition of the present invention on the number of writhing times induced by glacial acetic acid in mice

[0148]

[0149] Note: Compared with the blank group, * P<0.05, ** P<0.01

[0150] The experimental results in Table 2 show that compared with the blank group, the number of writhing times of mice in the positive control dexamethasone group was significantly reduced (P<0.05), and the writhing inhibition rate was 41.3%. Compared with the blank group, the number of writhing times of mice in the medium dose group and high dose group of the composition of the present invention were significantly reduced (P<0.05, P<0.01), and the writhing inhibition rates were 30.6% and 47.5%, respectively.

[0151] 3. Xylene-induced ear swelling in mice

[0152] 3.1 Experimental methods

[0153] After the acetic acid writhing experiment on mice, the animals were kept overnight (during the acetic acid writhing experiment, the right ear was also simultaneously coated with medicine (20 μL / mouse) while the abdomen of the mice was coated with medicine (30 μL / mouse)). The next day, the right auricle of the animal was smeared with medicine again, 20 μL / mouse, that is, the right ear was continuously administrated for 4 days in the xylene-induced mouse auricle swelling experiment. The blank group was given an equal volume of pure water. One hour after the end of the drug administration, 0.03 mL of xylene was evenly applied on both sides of the right ear of each group of mice, and the left ear was not coated as a control. One hour after the inflammation, the mice were killed by dislocating the cervical vertebra, and the same area of ​​both ears was cut off with a 7 mm diameter puncher, and the ear pieces were weighed. The difference in the weight of the two ear pieces was taken as the degree of swelling (swelling degree = right ear weight - left ear weight).

[0154] 3.2 Experimental Results

[0155] Table 3. Effect of the composition of the present invention on the swelling of the auricle of mice induced by xylene inflammation

[0156]

[0157] Note: Compared with the blank group, * P<0.05, ** P<0.01.

[0158] The experimental results in Table 3 show that compared with the blank group, the positive control dexamethasone group was administered for 4 days continuously, which significantly reduced the swelling of the auricle of mice induced by xylene inflammation (P<0.01). Compared with the blank group, the medium-dose and high-dose groups of the composition of the present invention significantly reduced the swelling of the auricle of mice induced by xylene (P<0.05, P<0.01).

[0159] In summary, under the experimental conditions and designed dosage, the composition of the present invention can be used for relieving itching and anti-inflammatory swelling after mosquito bites.

Claims

1. A composition for treating mosquito bites, characterized in that: The composition is made from the following raw materials: Patchouli 4-14 parts by weight, Atractylodes 9-28 parts by weight, Cinnamon 0.5-4 parts by weight, Nepeta 1-5 parts by weight, Elsholtzia 5-18 parts by weight, Artemisia argyi 11-33 parts by weight, Cloves 0.1-3 parts by weight, Zanthoxylum bungeanum 0.5-2 parts by weight, and Mint 2-10 parts by weight; The raw materials further contain 0.01-0.2 parts by weight of natural borneol; and the composition is in the form of volatile oil.

2. The method for preparing the composition for treating mosquito bites according to claim 1, characterized in that: The volatile oil is prepared by a subcritical water extraction method, comprising the following steps: The patchouli is crushed, and the crushed patchouli is extracted in water at an extraction pressure of 5 to 10 MPa and an extraction temperature of 140 to 160° C. for 30 to 40 minutes to obtain patchouli volatile oil. The Atractylodes lancea was crushed, and the crushed Atractylodes lancea was extracted in water for 50 to 60 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 150 to 170° C. to obtain Atractylodes lancea volatile oil. The cinnamon is crushed, and the crushed cinnamon is extracted in water at an extraction pressure of 5 to 10 MPa and an extraction temperature of 150 to 160° C. for 40 to 50 minutes to obtain cinnamon volatile oil. The schizonepeta is crushed, and the crushed schizonepeta is extracted in water at an extraction pressure of 5 to 10 MPa and an extraction temperature of 130 to 140° C. for 30 to 40 minutes to obtain schizonepeta volatile oil. The Elsholtzia ciliata was crushed, and the crushed Elsholtzia ciliata was extracted in water at an extraction pressure of 5 to 10 MPa and an extraction temperature of 140 to 160° C. for 30 to 40 minutes to obtain the Elsholtzia ciliata volatile oil. The wormwood leaf is crushed, and the crushed wormwood leaf is extracted in water for 30 to 40 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 135 to 145° C. to obtain wormwood leaf volatile oil. The cloves are crushed, and the crushed cloves are extracted in water at an extraction pressure of 5 to 10 MPa and an extraction temperature of 135 to 145° C. for 30 to 40 minutes to obtain clove volatile oil. The peppercorns are crushed, and the crushed peppercorns are extracted in water for 30 to 40 minutes at an extraction pressure of 5 to 10 MPa and an extraction temperature of 135 to 150° C. to obtain peppercorn volatile oil. The mint is crushed, and the crushed mint is extracted in water at an extraction pressure of 5 to 10 MPa and an extraction temperature of 135 to 145° C. for 30 to 40 minutes to obtain mint volatile oil. The volatile oils obtained from the above raw materials are mixed to obtain the total volatile oil; Alternatively, the method is prepared by subcritical water extraction, comprising the following steps: Patchouli, atractylodes, cinnamon, schizonepeta, elsholtzia, wormwood, clove, prickly ash, and mint are crushed and mixed, and the crushed raw material mixture is extracted in water at an extraction pressure of 5 to 10 MPa and an extraction temperature of 130 to 180° C. for 30 to 60 minutes to obtain total volatile oil; The preparation method of the composition comprises the following steps: adding natural borneol to the total volatile oil obtained from the above raw materials, stirring to completely dissolve the natural borneol, and obtaining the composition.

3. Use of the composition according to claim 1 or the composition prepared according to claim 2 in the preparation of a medicament for treating mosquito bites.

4. The use according to claim 3, characterized in that: The medicine for treating mosquito bites is in the form of spray, aerosol, liniment, cream, ointment, gel or solution.

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