Antibody turbidity detection method, device, and system

Abstract

The application relates to an antibody turbidity detection method, device, system, computer equipment, storage medium and computer program product, real-time acquisition of a transmission signal and a scattering signal after a light source irradiates a turbidimetric measurement container, the turbidimetric measurement container stores a to-be-measured liquid; a turbidity calculation function of the to-be-measured liquid is determined according to the transmission signal; and antibody turbidity of the to-be-measured liquid is determined according to the turbidity calculation function and the latest scattering signal. In the whole process, the turbidity interval is preliminarily estimated based on the transmission signal, the appropriate turbidity calculation function is determined, and then the turbidity calculation function and the scattering signal are used to detect the antibody turbidity, which can overcome the defect that the antibody turbidity is too high to cause false negatives, and accurate antibody turbidity detection is realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of intelligent detection, in particular to an antibody turbidity detection method, device, system, computer equipment, storage medium and computer program product. BACKGROUND

[0002] Traditional antibody turbidity detection mainly includes two kinds of scatter turbidimetry and transmission turbidimetry, which are widely used in environmental monitoring and medical testing fields. Taking the detection principle of immunoglobulin antibody as an example, when parallel light passes through the immune complex solution, part of the light is scattered in all directions due to the large volume and rough surface of the antigen-antibody reaction object. By collecting and analyzing the scattered light signal with a photodetector, the amount of antibody contained in the original system before the reaction can be obtained. Unlike transmission turbidimetry, scatter turbidimetry is less affected by particle light absorption effect, has a larger linear interval, and is more suitable for quantitative measurement.

[0003] Generally speaking, the ratio of antigen and antibody affects the reaction in the system, and the addition of antigen after reaching the maximum reaction degree will reduce the complex. In addition, as the turbidity increases, the intensity of the scattered light itself will also weaken.

[0004] Therefore, although the traditional scatter turbidimetry or transmission turbidimetry can realize the detection of antibody turbidity, the detection results obtained are not accurate. SUMMARY

[0005] Therefore, it is necessary to provide an accurate antibody turbidity detection method, device, system, computer equipment, storage medium and computer program product to solve the above technical problems.

[0006] In a first aspect, the present application provides an antibody turbidity detection method. The method comprises:

[0007] Real-time acquisition of transmission signals and scattering signals after the light source irradiates the turbidimetry container, and the turbidimetry container stores a to-be-measured liquid;

[0008] According to the transmission signal, a turbidity calculation function of the to-be-measured liquid is determined;

[0009] According to the turbidity calculation function and the latest scattering signal, the antibody turbidity of the to-be-measured liquid is determined.

[0010] In one embodiment, according to the transmission signal, the turbidity calculation function of the to-be-measured liquid comprises:

[0011] According to the transmission signal, a turbidity interval of the to-be-measured liquid is determined;

[0012] The scattering optical path corresponding to the turbidity interval is determined;

[0013] Obtaining a turbidity calculation function corresponding to the scattering light path.

[0014] In one embodiment, determining the scattering light path corresponding to the turbidity interval comprises:

[0015] Obtaining a turbidity-scattering light path correspondence;

[0016] According to the turbidity interval and the turbidity-scattering light path correspondence, determining the scattering light path corresponding to the turbidity interval.

[0017] In one embodiment, determining the turbidity calculation function of the to-be-measured liquid according to the transmission signal comprises:

[0018] Determining a reaction interval according to the transmission signal;

[0019] Obtaining a turbidity calculation function of the to-be-measured liquid corresponding to the reaction interval.

[0020] In one embodiment, determining the reaction interval according to the transmission signal comprises:

[0021] Obtaining a preset transmission signal threshold, the transmission signal threshold being obtained based on a scattering signal reaction curve of a standard turbidity liquid;

[0022] According to the preset transmission signal threshold, identifying a front reaction interval or a rear reaction interval in the scattering reaction.

[0023] In a second aspect, the application also provides an antibody turbidity detection device. The device comprises a light source, a turbidimetric measurement container, a transmission signal detection module, a scattering signal detection module, and a control module. The turbidimetric measurement container stores a to-be-measured liquid. The light source and the transmission signal detection module are arranged on opposite sides of the turbidimetric measurement container, and the transmission signal detection module is on the light path of the light source. The control module is connected with the transmission signal detection module and the scattering signal detection module. The light source irradiates the turbidimetric measurement container, the transmission signal detection module collects the transmission signal, the scattering signal detection module collects the scattering signal, and the control module obtains the transmission signal and the scattering signal in real time. According to the transmission signal, the turbidity calculation function of the to-be-measured liquid is determined. According to the turbidity calculation function and the latest scattering signal, the antibody turbidity of the to-be-measured liquid is determined.

[0024] In one embodiment, the control module is further configured to determine a turbidity interval of the to-be-measured liquid according to the transmission signal, obtain a scattering light path corresponding to the turbidity interval, and adjust the light path of the scattering signal detection module according to the scattering light path.

[0025] In one embodiment, the turbidimetric measurement container is a cuvette, and the transmittance of the light source emitted by the turbidimetric measurement container is higher than a preset transmittance threshold.

[0026] In one of the embodiments, the transmission signal detection module and the scattering signal detection module are the same type of optical signal detection module.

[0027] In a third aspect, the present application further provides an antibody turbidity detection system, which comprises:

[0028] The acquisition module is configured to acquire the transmission signal and the scattering signal in real time after the light source irradiates the turbidimetric measurement container, and the turbidimetric measurement container stores the to-be-tested liquid.

[0029] The function calculation module is configured to determine a turbidity calculation function of the to-be-tested liquid according to the transmission signal.

[0030] The turbidity determination module is configured to determine the antibody turbidity of the to-be-tested liquid according to the turbidity calculation function and the latest scattering signal.

[0031] In a fourth aspect, the present application further provides a computer device. The computer device comprises a memory and a processor, and the memory stores a computer program. When the processor executes the computer program, the following steps are implemented:

[0032] The acquisition module is configured to acquire the transmission signal and the scattering signal in real time after the light source irradiates the turbidimetric measurement container, and the turbidimetric measurement container stores the to-be-tested liquid.

[0033] The function calculation module is configured to determine a turbidity calculation function of the to-be-tested liquid according to the transmission signal.

[0034] The turbidity determination module is configured to determine the antibody turbidity of the to-be-tested liquid according to the turbidity calculation function and the latest scattering signal.

[0035] In a fifth aspect, the present application further provides a computer readable storage medium. The computer readable storage medium stores a computer program, and when the computer program is executed by a processor, the following steps are implemented:

[0036] The acquisition module is configured to acquire the transmission signal and the scattering signal in real time after the light source irradiates the turbidimetric measurement container, and the turbidimetric measurement container stores the to-be-tested liquid.

[0037] The function calculation module is configured to determine a turbidity calculation function of the to-be-tested liquid according to the transmission signal.

[0038] The turbidity determination module is configured to determine the antibody turbidity of the to-be-tested liquid according to the turbidity calculation function and the latest scattering signal.

[0039] In a sixth aspect, the present application further provides a computer program product. The computer program product comprises a computer program, and when the computer program is executed by a processor, the following steps are implemented:

[0040] The acquisition module is configured to acquire the transmission signal and the scattering signal in real time after the light source irradiates the turbidimetric measurement container, and the turbidimetric measurement container stores the to-be-tested liquid.

[0041] The function calculation module is configured to determine a turbidity calculation function of the to-be-tested liquid according to the transmission signal.

[0042] According to the turbidity calculation function and the latest scattering signal, the antibody turbidity of the to-be-tested liquid is determined.

[0043] The antibody turbidity detection method, device, system, computer device, storage medium and computer program product provided in the above embodiment can acquire the transmission signal and the scattering signal after the light source irradiates the turbidimetric measurement container in real time, and the to-be-tested liquid is stored in the turbidimetric measurement container; according to the transmission signal, the turbidity calculation function of the to-be-tested liquid is determined; and according to the turbidity calculation function and the latest scattering signal, the antibody turbidity of the to-be-tested liquid is determined. In the whole process, the turbidity range is preliminarily estimated based on the transmission signal, and then the appropriate turbidity calculation function is determined, and the antibody turbidity is detected by using the turbidity calculation function and the scattering signal, which can overcome the defect of false negative caused by too high antibody turbidity, and realize accurate antibody turbidity detection. BRIEF DESCRIPTION OF DRAWINGS

[0044] Figure 1 It is an application environment diagram of the antibody turbidity detection method in an embodiment;

[0045] Figure 2 It is a flowchart of the antibody turbidity detection method in an embodiment;

[0046] Figure 3 It is a structural diagram of the antibody turbidity detection device in an embodiment;

[0047] Figure 4 It is a structural diagram of the antibody turbidity detection device in another embodiment;

[0048] Figure 5 It is a structural block diagram of the antibody turbidity detection device in an embodiment;

[0049] Figure 6 It is a schematic diagram of the antibody turbidity detection process in an embodiment;

[0050] Figure 7 It is an internal structure diagram of the computer device in an embodiment. DETAILED DESCRIPTION

[0051] In order to make the purpose, technical scheme and advantages of the present application clearer, the present application will be further described in detail below with reference to the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and not to limit the present application.

[0052] The antibody turbidity detection method provided in the embodiments of the present application can be applied to, for example, Figure 1The application environment shown. Among them, the terminal 102 communicates with the server 104 through the network. The data storage system can store the data required by the server 104 to process. The data storage system can be integrated on the server 104, or placed on the cloud or other network servers. The terminal 102 sends an antibody turbidity detection request to the server 104, which carries the transmission signal and scattering signal after the light source irradiates the turbidimetric measurement container. The server 104 acquires the transmission signal and scattering signal after the light source irradiates the turbidimetric measurement container in real time, determines the turbidity calculation function of the measured liquid according to the transmission signal, and determines the antibody turbidity of the measured liquid according to the turbidity calculation function and the latest scattering signal. Among them, the terminal 102 can be, but not limited to, various personal computers, notebook computers, smart phones, tablet computers, Internet of Things devices and portable wearable devices. The Internet of Things device can be a smart speaker, a smart TV, a smart air conditioner, a smart vehicle device, etc. The portable wearable device can be a smart watch, a smart bracelet, a head-mounted device, etc. The server 104 can be realized by an independent server or a server cluster composed of multiple servers.

[0053] In one embodiment, as Figure 2 shown, an antibody turbidity detection method is provided, which is applied to Figure 1 the server 104 in the above application environment for example, including the following steps:

[0054] S200: Real-time acquisition of transmission signal and scattering signal after light source irradiates turbidimetric measurement container, and the turbidimetric measurement container stores the measured liquid.

[0055] The light source refers to an object that emits light. The measured liquid is stored in the turbidimetric measurement container, and the light source emits light to irradiate the turbidimetric measurement container. The light occurs transmission reaction and scattering reaction, and the corresponding transmission signal and scattering signal are collected in real time for the next turbidity detection. In actual application, the transmission signal and scattering signal can be detected by a transmission signal detection module and a scattering signal detection module, respectively, wherein the transmission signal detection module and the light source are located on the opposite sides of the turbidimetric measurement container, and the transmission signal detection module is located on the central light path of the light source. The scattering signal detection module can be arranged on the perpendicular line of the central light path.

[0056] S400: According to the transmission signal, determine the turbidity calculation function of the measured liquid.

[0057] The transmission signal can be understood as a one-way decreasing function related to the turbidity N of the liquid to be measured, and thus the approximate turbidity interval can be determined based on the transmission signal. There are two directions for determining the turbidity calculation function of the liquid to be measured based on the transmission signal: one is that different turbidity intervals correspond to different reasonable scattering light paths (the corresponding relationship between the two can be obtained by testing standard turbidity solutions), and the corresponding turbidity calculation functions of different scattering light paths are different in the final calculation of turbidity. After the scattering light path is determined based on the transmission signal, the final turbidity calculation function is determined based on the scattering light path; the second is that the scattering signal reaction interval can be determined based on the transmission signal, and the corresponding turbidity calculation function is selected based on the scattering signal reaction interval.

[0058] S600: determining the antibody turbidity of the liquid to be measured according to the turbidity calculation function and the latest scattering signal.

[0059] The latest scattering signal refers to the scattering signal collected at the current time. In the process of obtaining the turbidity calculation function of the liquid to be measured in S400, the scattering light path may be adjusted, and the corresponding scattering signal changes after the scattering light path changes. Therefore, the latest scattering signal needs to be obtained. The antibody turbidity of the liquid to be measured is calculated according to the turbidity calculation function and the latest scattering signal.

[0060] The above antibody turbidity detection method, the transmission signal and the scattering signal after the light source irradiates the turbidimetric measurement container are obtained in real time, and the liquid to be measured is stored in the turbidimetric measurement container; the turbidity calculation function of the liquid to be measured is determined according to the transmission signal; the antibody turbidity of the liquid to be measured is determined according to the turbidity calculation function and the latest scattering signal. In the whole process, the turbidity interval is preliminarily estimated based on the transmission signal, and then the appropriate turbidity calculation function is determined, and the antibody turbidity is detected by the turbidity calculation function and the scattering signal. It can overcome the defect of false negative caused by high antibody turbidity, and realize accurate antibody turbidity detection.

[0061] In one embodiment, determining the turbidity calculation function of the liquid to be measured according to the transmission signal comprises:

[0062] The turbidity interval of the liquid to be measured is determined according to the transmission signal; the scattering light path corresponding to the turbidity interval is determined; and the turbidity calculation function corresponding to the scattering light path is obtained.

[0063] In the embodiment, the distance (scattering path) grading is used to detect the turbidity of the liquid to be measured, that is, the turbidity calculation function based on the scattering path is obtained. As described above, the transmission signal can determine the approximate turbidity interval, and the corresponding reasonable scattering path is selected based on the turbidity interval, and the turbidity calculation function based on the reasonable scattering path is obtained. It can be understood that after the turbidity interval is determined based on the transmission signal, and the corresponding scattering path is determined, the scattering path of the scattering signal acquisition can be adjusted, and the scattering signal of the reacquired signal is obtained. Subsequently, the turbidity of the liquid to be measured is detected based on the updated scattering signal and the determined turbidity calculation function.

[0064] In one of the embodiments, determining the scattering path corresponding to the turbidity interval comprises:

[0065] obtaining the corresponding relationship between turbidity and scattering path; and determining the scattering path corresponding to the turbidity interval according to the turbidity interval and the corresponding relationship between turbidity and scattering path.

[0066] The corresponding relationship between turbidity and scattering path is a corresponding relationship constructed in advance based on experimental data, which represents the reasonable scattering path corresponding to different turbidity intervals. Specifically, in actual application, when the transmission signal is lower than a certain threshold k, a smaller scattering signal may correspond to two turbidity values (hook effect), at this time, the scattering path L n is increased by one gear, so that the scattering path L n is reduced, the scattering signal I s is increased, and the specific corresponding relationship between turbidity and scattering path can be calibrated by measuring a standard turbidity solution.

[0067] In one of the embodiments, determining the turbidity calculation function of the liquid to be measured according to the transmission signal comprises: determining the reaction interval according to the transmission signal; and obtaining the turbidity calculation function of the liquid to be measured corresponding to the reaction interval.

[0068] In the calibration test process, the initial scattering path L n corresponds to the gear 1, and the gear n is sequentially increased until the scattering signal is in the linear interval h(n)·X, and the corresponding turbidity is calculated according to the nth segment function. The specific segment function is as follows:

[0069]

[0070]

[0071] In one of the embodiments, determining the reaction interval according to the transmission signal comprises:

[0072] The preset transmission signal threshold is obtained based on a scattering signal reaction curve of a standard turbidity liquid. The preset transmission signal threshold is used to identify whether the sample is in the front band reaction interval or the rear band reaction interval.

[0073] The preset transmission signal threshold is a preset threshold signal, which is obtained based on a scattering signal reaction curve of a standard turbidity liquid. The preset transmission signal threshold is influenced by a light source, a transmission signal detector, a scattering signal detector and a scattering light path, and is mainly influenced by the scattering light path. Based on the transmission signal threshold, the front and rear bands of the scattering reaction curve can be divided, and the false negative problem can be solved.

[0074] In the embodiment, a segmented measurement mode based on the transmission signal is adopted, and the scattering light path L n is fixed. T The preset transmission signal threshold k is used as a demarcation point to distinguish the front band and the rear band of the scattering reaction. Two different function relationship formulas of the turbidity and the scattering signal value I S are respectively used for the front band and the rear band. The function relationship formulas are as follows:

[0075]

[0076] As shown in Figure 3 , the application further provides an antibody turbidity detection device. The device includes a light source 1, a turbidimetric measurement container 2, a transmission signal detection module 3, a scattering signal detection module 4 and a control module 5. The turbidimetric measurement container 2 stores a sample to be measured. The light source 1 and the transmission signal detection module 3 are arranged on opposite sides of the turbidimetric measurement container 2, and the transmission signal detection module 3 is located on the light path of the light source 1. The control module 5 is connected with the transmission signal detection module 3 and the scattering signal detection module 4. The light source 1 irradiates the turbidimetric measurement container 2. The transmission signal detection module 3 collects the transmission signal, and the scattering signal detection module 4 collects the scattering signal. The control module 5 obtains the transmission signal and the scattering signal in real time, determines a turbidity calculation function of the sample to be measured according to the transmission signal, and determines the antibody turbidity of the sample to be measured according to the turbidity calculation function and the latest scattering signal.

[0077] The light source 1 is a device for emitting light. The light source 1 emits light of a specific wavelength to the turbidimetric measurement container 2. The light is transmitted and scattered, and is detected by the transmission signal detection module 3 and the scattering signal detection module 4. Specifically, the light source 1 can be a near-infrared light source 1, which can reduce the absorption of the light source 1 by the complex as much as possible and improve the signal-to-noise ratio of the scattering signal. Further, the light source 1 can be a near-infrared light-emitting diode or a laser diode (preferably, the wavelength is 850 nm to 950 nm), which has good monochromaticity and collimation and can be directly used in the turbidimetric detection light path.

[0078] The turbidimetric measurement container 2 is used to store the liquid to be measured, and can be designed in shape and capacity according to actual application scenarios or experimental personnel preferences. Further, in order to reduce the sample volume and accelerate the reaction, the turbidimetric measurement container 2 can be a cuvette, and the cuvette is transparent on four sides. Further, the turbidimetric measurement container 2 can adopt a cuvette with a light transmittance higher than a preset light transmittance threshold. The preset light transmittance threshold realizes a set threshold, which can be set according to actual conditions, for example, can be 95%, 98%, etc. It can be understood that the higher the light transmittance of the cuvette, the higher the detection accuracy of the entire antibody turbidity detection device. On the contrary, if the light transmittance of the cuvette is low, it will affect the signal value, and may also cause the signal-to-noise ratio of the scattered signal to decrease.

[0079] The transmission signal detection module 3 is used to detect the transmission signal, and the transmission signal detection module 3 and the light source 1 are arranged on opposite sides of the turbidimetric measurement container 2. The transmission signal detection module 3 is on the light path of the light source 1, and is preferably on the central light path, that is, the transmission signal detection module 3 and the light source 1 are on the same horizontal line. The scattering signal detection module 4 is used to detect the scattering signal, and can be arranged on a vertical line of the line connecting the transmission signal detection module 3 and the light source 1. According to the azimuthal angle, the light source 1, the transmission signal detection module 3 and the scattering signal detection module 4 are respectively located at the left, right and lower positions with the turbidimetric measurement container 2 as the reference. Further, in order to facilitate subsequent data processing, the transmission signal detection module 3 and the scattering signal detection module 4 can be the same type of signal detection module (circuit), and more specifically, both can be silicon photodiodes of the same type, and the A / D sampling amplification circuit is also the same. This facilitates analysis of the two signals on the same reference line and calculation of the corresponding turbidity value.

[0080] The control module 5 is used to calculate the final turbidity (concentration) of the liquid to be measured according to the received transmission signal and scattering signal. Specifically, the control module 5 is used to acquire the transmission signal and the scattering signal in real time, determine the turbidity calculation function of the liquid to be measured according to the transmission signal, and determine the antibody turbidity of the liquid to be measured according to the turbidity calculation function and the latest scattering signal. In actual application, the control module 5 can be a central processing unit or an upper computer.

[0081] The above antibody turbidity detection device acquires the transmission signal and the scattering signal after the light source 1 irradiates the turbidimetric measurement container 2 in real time, and the turbidimetric measurement container 2 stores the liquid to be measured. According to the transmission signal, the turbidity calculation function of the liquid to be measured is determined. According to the turbidity calculation function and the latest scattering signal, the antibody turbidity of the liquid to be measured is determined. In the whole process, the turbidity interval is preliminarily estimated based on the transmission signal, and then the appropriate turbidity calculation function is determined. The antibody turbidity is detected by the turbidity calculation function and the scattering signal, which can overcome the defect of false negative caused by high antibody turbidity, and realize accurate antibody turbidity detection.

[0082] In one embodiment, the control module 5 is further configured to determine a turbidity interval of the liquid to be measured according to the transmission signal, obtain a scattering optical path corresponding to the turbidity interval, and adjust the optical path of the scattering signal detection module 4 according to the scattering optical path.

[0083] In this embodiment, the antibody turbidity detection device adopts a segmented measurement. Specifically, the control module 5 first analyzes the transmission signal to determine the turbidity interval of the liquid to be measured, obtains the scattering optical path corresponding to the turbidity interval based on the turbidity-scattering optical path correspondence, and then adjusts the optical path (scattering optical path) of the scattering signal detection module 4. In actual application, this adjustment process is as shown in Figure 4 The light source 1 is moved between the transmission signal detection module 3 at intervals (from the solid line position to the dotted line position) to adjust the transmission and scattering optical path L n The turbidity detection of different turbidity compounds is adapted by using different fitting functions to identify different turbidity segments. I is the final fitting turbidity signal value segmented linear equation, and the specific equation is as follows:

[0084]

[0085]

[0086] Further, the antibody turbidity detection device can also adopt a segmented measurement. The control module 5 obtains the transmission signal and the scattering signal in real time, determines the reaction interval according to the transmission signal, obtains the turbidity calculation function of the liquid to be measured corresponding to the reaction interval, and calculates the turbidity of the liquid to be measured according to the scattering signal and the determined turbidity calculation function of the liquid to be measured. Specifically, based on the segmented measurement of the transmission signal, the scattering optical path L n is fixed, and the transmission signal I T is divided into two segments before and after the scattering reaction with a preset transmission signal threshold k. There are two different fitting functions of turbidity and scattering signal value I S for the front and rear bands. The function relationship is as follows:

[0087]

[0088] As shown in Figure 5 The application further provides an antibody turbidity detection system. The system comprises:

[0089] The acquisition module 200 is configured to obtain the transmission signal and the scattering signal after the light source irradiates the turbidimetric measurement container in real time. The turbidimetric measurement container stores the liquid to be measured.

[0090] The function calculation module 400 is configured to determine the turbidity calculation function of the liquid to be measured according to the transmission signal.

[0091] The turbidity determination module 600 is configured to determine the antibody turbidity of the liquid to be measured according to a turbidity calculation function and the latest scattering signal.

[0092] In one of the embodiments, the function calculation module 400 is further configured to determine a turbidity interval of the liquid to be measured according to the transmission signal; determine a scattering optical path corresponding to the turbidity interval; and obtain the turbidity calculation function corresponding to the scattering optical path.

[0093] In one of the embodiments, the function calculation module 400 is further configured to obtain a correspondence between turbidity and scattering optical path; and determine the scattering optical path corresponding to the turbidity interval according to the turbidity interval and the correspondence between turbidity and scattering optical path.

[0094] In one of the embodiments, the function calculation module 400 is further configured to determine a reaction interval according to the transmission signal; and obtain the turbidity calculation function of the liquid to be measured corresponding to the reaction interval.

[0095] In one of the embodiments, the function calculation module 400 is further configured to obtain a preset transmission signal threshold value, the transmission signal threshold value being obtained based on a scattering signal reaction curve of a standard turbidity liquid; and identify the front reaction interval or the rear reaction interval according to the preset transmission signal threshold value.

[0096] To make the technical scheme of the antibody turbidity detection method, device and system of the present application more clear, the following will describe the technical scheme with specific examples. As shown in the actual application, the whole antibody turbidity detection process is as follows: Figure 6

[0097] 1. Add the liquid to be measured into the turbidimetric measurement container, and irradiate the turbidimetric measurement container with the light source to cause transmission reaction and scattering reaction;

[0098] 2. If the present measurement is in the form of grading, the following operations are performed:

[0099] 2.1. Determine the turbidity interval according to the transmission signal I T ;

[0100] 2.2. Determine whether it is necessary to switch the gear to adjust the scattering optical path L n , if yes, calculate the scattering optical path L n corresponding to the turbidity, and adjust to the corresponding gear, and then re-collect the scattering signal after the adjustment of the scattering optical path; if not, directly use the scattering signal I s already collected;

[0101] 2.2. Obtain the final turbidity of the liquid to be measured based on the following turbidity signal value piecewise linear equation.

[0102]

[0103] ​

[0104] 3. If the segmented measurement is taken, the following operations are performed:

[0105] 3.1. Determine the reaction interval by the transmission signal;

[0106] 3.2. Based on different reaction intervals, select the corresponding turbidity calculation function, which is as follows:

[0107]

[0108] It should be understood that, although each step in the flowchart involved in each of the above embodiments is shown in sequence according to the arrow, these steps are not necessarily executed in sequence according to the arrow. Unless explicitly stated herein, there is no strict order restriction for the execution of these steps, and these steps can be executed in other orders. Moreover, at least part of the steps in the flowchart involved in each of the above embodiments can include multiple steps or multiple stages, which are not necessarily executed at the same time, but can be executed at different times, and the execution order of these steps or stages is not necessarily sequential, but can be alternately executed with at least part of other steps or steps or stages in other steps.

[0109] In an embodiment, a computer device is provided, which can be a server, and an internal structure diagram thereof can be as shown in Figure 7 The computer device includes a processor, a memory and a network interface connected through a system bus. The processor of the computer device is configured to provide computing and control capabilities. The memory of the computer device includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system, a computer program and a database. The internal memory provides an environment for the operating system and the computer program in the non-volatile storage medium to run. The database of the computer device is configured to store preset threshold data. The network interface of the computer device is configured to communicate with an external terminal through a network connection. The computer program is executed by the processor to implement an antibody turbidity detection method.

[0110] Those skilled in the art can understand that Figure 7 the structure shown in the figure is only a block diagram of part of the structure related to the scheme of the present application, and does not constitute a limitation on the computer device to which the scheme of the present application is applied. The specific computer device can include more or fewer components than those shown in the figure, or combine certain components, or have a different arrangement of components.

[0111] In an embodiment, a computer device is provided, which includes a memory and a processor, and the memory stores a computer program. The processor executes the computer program to implement the following steps:

[0112] acquire the transmission signal and the scattering signal in real time after the turbidimetric measuring container is irradiated by the light source, and the turbidimetric measuring container stores the to-be-measured liquid;

[0113] determine a turbidity calculation function of the to-be-measured liquid according to the transmission signal;

[0114] determine the antibody turbidity of the to-be-measured liquid according to the turbidity calculation function and the latest scattering signal.

[0115] In an embodiment, the processor, when executing the computer program, further implements the following steps:

[0116] determine a turbidity interval of the to-be-measured liquid according to the transmission signal; determine a scattering optical path corresponding to the turbidity interval; and acquire a turbidity calculation function corresponding to the scattering optical path.

[0117] In an embodiment, the processor, when executing the computer program, further implements the following steps:

[0118] acquire a correspondence between turbidity and scattering optical path; and determine a scattering optical path corresponding to the turbidity interval according to the turbidity interval and the correspondence between turbidity and scattering optical path.

[0119] In an embodiment, the processor, when executing the computer program, further implements the following steps:

[0120] determine a reaction interval according to the transmission signal; and acquire a turbidity calculation function of the to-be-measured liquid corresponding to the reaction interval.

[0121] In an embodiment, the processor, when executing the computer program, further implements the following steps:

[0122] acquire a preset transmission signal threshold, the transmission signal threshold being obtained based on a scattering signal reaction curve of a standard turbidity liquid; and identify a front band reaction interval or a rear band reaction interval according to the preset transmission signal threshold.

[0123] In an embodiment, a computer readable storage medium is provided, and the computer readable storage medium stores a computer program. The computer program, when executed by a processor, implements the following steps:

[0124] acquire the transmission signal and the scattering signal in real time after the turbidimetric measuring container is irradiated by the light source, and the turbidimetric measuring container stores the to-be-measured liquid;

[0125] determine a turbidity calculation function of the to-be-measured liquid according to the transmission signal;

[0126] determine the antibody turbidity of the to-be-measured liquid according to the turbidity calculation function and the latest scattering signal.

[0127] In an embodiment, the computer program, when executed by the processor, further implements the following steps:

[0128] determine a turbidity interval of the liquid to be measured according to the transmission signal; determine a scattering optical path corresponding to the turbidity interval; and obtain a turbidity calculation function corresponding to the scattering optical path.

[0129] In one embodiment, the computer program, when executed by the processor, further implements the following steps:

[0130] obtain a correspondence between turbidity and scattering optical path; and determine the scattering optical path corresponding to the turbidity interval according to the turbidity interval and the correspondence between turbidity and scattering optical path.

[0131] In one embodiment, the computer program, when executed by the processor, further implements the following steps:

[0132] determine a reaction interval according to the transmission signal; and obtain a turbidity calculation function of the liquid to be measured corresponding to the reaction interval.

[0133] In one embodiment, the computer program, when executed by the processor, further implements the following steps:

[0134] obtain a preset transmission signal threshold, the transmission signal threshold being obtained based on a scattering signal reaction curve of a standard turbidity liquid; and identify a front reaction interval or a rear reaction interval in the scattering reaction according to the preset transmission signal threshold.

[0135] In one embodiment, a computer program product is provided, comprising a computer program which, when executed by a processor, implements the following steps:

[0136] obtain the transmission signal and the scattering signal in real time after the light source irradiates the turbidimetric measurement container, the turbidimetric measurement container storing the liquid to be measured;

[0137] determine a turbidity calculation function of the liquid to be measured according to the transmission signal;

[0138] determine the antibody turbidity of the liquid to be measured according to the turbidity calculation function and the latest scattering signal.

[0139] In one embodiment, the computer program, when executed by the processor, further implements the following steps:

[0140] determine a turbidity interval of the liquid to be measured according to the transmission signal; determine a scattering optical path corresponding to the turbidity interval; and obtain a turbidity calculation function corresponding to the scattering optical path.

[0141] In one embodiment, the computer program, when executed by the processor, further implements the following steps:

[0142] obtain a correspondence between turbidity and scattering optical path; and determine the scattering optical path corresponding to the turbidity interval according to the turbidity interval and the correspondence between turbidity and scattering optical path.

[0143] In one embodiment, the computer program, when executed by the processor, further implements the following steps:

[0144] According to the transmission signal, a reaction interval is determined, and a turbidity calculation function of the to-be-tested liquid corresponding to the reaction interval is obtained.

[0145] In one embodiment, the computer program, when executed by the processor, further implements the following steps:

[0146] A preset transmission signal threshold is obtained, the transmission signal threshold is obtained based on a scattering signal reaction curve of a standard turbidity liquid, and according to the preset transmission signal threshold, a front band reaction interval or a rear band reaction interval in the scattering reaction is identified.

[0147] It should be noted that the user information (including but not limited to user equipment information, user personal information, etc.) and data (including but not limited to data for analysis, stored data, displayed data, etc.) involved in the present application are all information and data authorized by the user or authorized by all parties.

[0148] Those skilled in the art can understand that all or part of the processes in the above-mentioned embodiment methods can be completed by instructing the relevant hardware through a computer program. The computer program can be stored in a non-volatile computer readable storage medium, and when the computer program is executed, the processes of the above-mentioned embodiments of the methods can be included. Any reference to memory, database or other medium used in the embodiments provided in the present application can include at least one of non-volatile and volatile memory. The non-volatile memory can include read-only memory (ROM), magnetic tape, floppy disk, flash memory, optical storage, high-density embedded non-volatile memory, resistive memory (ReRAM), magnetoresistive random access memory (MRAM), ferroelectric memory (FRAM), phase change memory (PCM), graphene memory, etc. The volatile memory can include random access memory (RAM) or external cache memory, etc. As an illustration but not limitation, the RAM can be in various forms, such as static random access memory (SRAM) or dynamic random access memory (DRAM), etc. The database involved in the embodiments provided in the present application can include at least one of a relational database and a non-relational database. The non-relational database can include a distributed database based on a block chain, etc., without being limited thereto. The processor involved in the embodiments provided in the present application can be a general-purpose processor, a central processing unit, a graphics processing unit, a digital signal processor, a programmable logic device, a data processing logic device based on quantum computing, etc., without being limited thereto.

[0149] Any combination of the technical features of the above embodiments can be made. In order to make the description simple, all possible combinations of the technical features in the above embodiments are not described, however, as long as the combination of the technical features does not exist, it should be considered as the scope of the present application.

[0150] The above embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the patent of the present application. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are all within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the appended claims.

Claims

1. A method for detecting antibody turbidity, characterized in that, The method includes: The transmission and scattering signals of the turbidimetric measuring container after being illuminated by a light source are acquired in real time, and the turbidimetric measuring container contains the liquid to be tested; Based on the transmission signal, determine the turbidity calculation function of the test liquid; The antibody turbidity of the test solution is determined based on the turbidity calculation function and the latest scattering signal. The step of determining the turbidity calculation function of the test liquid based on the transmission signal includes: determining the turbidity range of the test liquid based on the transmission signal; determining the scattering optical path corresponding to the turbidity range; and obtaining the turbidity calculation function corresponding to the scattering optical path. Alternatively, determining the turbidity calculation function of the test liquid based on the transmission signal includes: obtaining a preset transmission signal threshold, the transmission signal threshold being obtained based on the scattering signal response curve of a standard turbid liquid; identifying the front-zone or back-zone reaction interval of the scattering reaction in the transmission signal using the preset transmission signal threshold as a dividing point; and obtaining the turbidity calculation function of the test liquid corresponding to the reaction interval.

2. The method according to claim 1, characterized in that, Determining the scattering optical path corresponding to the turbidity range includes: Obtain the correspondence between turbidity and scattered optical path length; Based on the turbidity range and the correspondence between turbidity and scattered optical path, the scattered optical path corresponding to the turbidity range is determined.

3. An antibody turbidity detection device, characterized in that, It includes a light source, a turbidimetric measuring container, a transmission signal detection module, a scattering signal detection module, and a control module, wherein the turbidimetric measuring container stores the liquid to be tested; The light source and the transmission signal detection module are disposed on opposite sides of the turbidimetric measuring container, and the transmission signal detection module is located in the light path emitted by the light source. The control module is connected to both the transmission signal detection module and the scattering signal detection module. The light source illuminates the turbidimetric measuring container, the transmission signal detection module collects the transmission signal, the scattering signal detection module collects the scattering signal, the control module acquires the transmission signal and the scattering signal in real time, and determines the turbidity calculation function of the test solution based on the transmission signal; and determines the antibody turbidity of the test solution based on the turbidity calculation function and the latest scattering signal. The step of determining the turbidity calculation function of the test liquid based on the transmission signal includes: determining the turbidity range of the test liquid based on the transmission signal; determining the scattering optical path corresponding to the turbidity range; and obtaining the turbidity calculation function corresponding to the scattering optical path. Alternatively, determining the turbidity calculation function of the test liquid based on the transmission signal includes: obtaining a preset transmission signal threshold, the transmission signal threshold being obtained based on the scattering signal response curve of a standard turbid liquid; identifying the front-zone or back-zone reaction interval of the scattering reaction in the transmission signal using the preset transmission signal threshold as a dividing point; and obtaining the turbidity calculation function of the test liquid corresponding to the reaction interval.

4. The apparatus according to claim 3, characterized in that, The turbidimetric measuring container is a cuvette, and the turbidimetric measuring container has a light transmittance to the light emitted by the light source that is higher than a preset transmittance threshold.

5. The apparatus according to claim 3, characterized in that, The transmitted signal detection module and the scattered signal detection module are optical signal detection modules of the same model.

6. The apparatus according to claim 3, characterized in that, The wavelength of the light source is 850nm~950nm.

7. The apparatus according to claim 4, characterized in that, The preset transmittance threshold is 95%.

8. The apparatus according to claim 3, characterized in that, The transmission signal detection module is located in the central optical path of the light source.

9. The apparatus according to claim 3, characterized in that, The transmission signal detection module and the scattering signal detection module are both silicon photodiodes of the same type.

10. An antibody turbidity detection system, characterized in that, The system includes: The acquisition module is used to acquire in real time the transmission signal and the scattering signal after the light source illuminates the turbidity measurement container, which contains the liquid to be tested; The function calculation module is used to determine the turbidity calculation function of the test liquid based on the transmission signal. The turbidity determination module is used to determine the antibody turbidity of the test solution based on the turbidity calculation function and the latest scattering signal. The function calculation module is also used to determine the turbidity range of the test liquid based on the transmission signal; determine the scattering optical path corresponding to the turbidity range; and obtain the turbidity calculation function corresponding to the scattering optical path. Alternatively, the function calculation module is also used to obtain a preset transmission signal threshold, which is obtained based on the scattering signal response curve of a standard turbid liquid; in the transmission signal, the preset transmission signal threshold is used as a dividing point to identify the front-zone or back-zone reaction interval of the scattering reaction; and to obtain the turbidity calculation function of the test liquid corresponding to the reaction interval.

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