A compound composition and its application in agriculture

Abstract

The present invention relates to a composite composition and its application in agriculture. Specifically, the present invention relates to a fungicide composition comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is selected from polyoxin, and the active ingredient B is selected from any one or both of cyfluthrin and epoxiconazole. Experiments have shown that the fungicide composition of the present invention exhibits significant synergistic effects and has excellent control effects on plant diseases, particularly potato late blight, tomato late blight, and cucumber downy mildew.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticide compounding, and in particular to a compounded composition and / or formulation comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is selected from polyoxin and the active ingredient B is selected from a triazole sulfonamide fungicide; the triazole sulfonamide fungicide is either one or both of cyfluthrin and epoxiconazole. The compounded composition and / or formulation exhibits significant synergistic effects in preventing and controlling plant diseases. Background Art

[0002] Polyoxin B is a metabolite produced by Streptomyces aureus. It is a broad-spectrum agricultural antibiotic fungicide with excellent systemic activity. Its mechanism of action is to interfere with the biosynthesis of chitin in the bacterial cell wall, preventing the biosynthesis of the bacterial cell wall and leading to the death of the pathogen. Polyoxin B is widely used to control diseases such as powdery mildew, gray mold, blight, anthrax, black spot, wilt, sheath blight, rust, and brown spot in fruits, vegetables, cereals, and fruit trees. It is also effective against cucumber downy mildew, powdery mildew, ginseng black spot, apple leaf spot, pear gray spot, wheat powdery mildew, and rice sheath blight. Its CAS number is 19396-06-6, and its structural formula is:

[0003]

[0004] Cyflumetoazole and epoxiconazole are new green and efficient fungicides developed by Dongguan Sunshine Pesticide R&D Co., Ltd. in Guangdong Province. They have good control effects on plant diseases such as downy mildew and blight, providing an effective new option for plant disease control. The chemical name of cyflumetoazole is: N 3 -cyclopropyl-N 1 ,N 1 -dimethyl-N 3 -(3-(trifluoromethyl)benzyl)-1H-1,2,4-triazole-1,3-disulfonamide, the structural formula is:

[0005]

[0006] The chemical name of epoxiconazole is: N 3 -(3-Bromobenzyl)-N 3 -cyclopropyl-N 1 ,N 1 -Dimethyl-1H-1,2,4-triazole-1,3-disulfonamide, the structural formula is:

[0007]

[0008] Plants are often attacked by diseases during their growth. If left uncontrolled, the resulting yield reductions and harvest losses can have serious adverse impacts on the socioeconomic landscape. Currently, the use of chemical pesticides is undoubtedly one of the most effective methods for controlling plant diseases. However, the long-term, repeated use of a single pesticide and other irregular usage practices can lead to the rapid development of disease resistance to the pesticides, resulting in increased pesticide usage, pesticide residues, environmental pollution, and other problems. Scientifically combining different pesticides can achieve positive results, such as expanding the control spectrum, improving efficacy, reducing dosage, and delaying the development of resistance. Summary of the Invention

[0009] Based on the above situation, the present invention provides a composite composition of polyoxin and triazole sulfonamide fungicides, which has a significant synergistic effect on plant diseases, especially on Oomycete diseases such as cucumber downy mildew and potato late blight, and can delay the development of resistance while improving the prevention effect.

[0010] Specifically:

[0011] In a first aspect, the present invention provides a composition comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is polyoxin and the active ingredient B is a triazole sulfonamide fungicide; wherein the triazole sulfonamide fungicide is selected from any one or both of cyfluthrin and epoxiconazole.

[0012] In some embodiments, the present invention provides a composition, wherein the active ingredients of the composition are active ingredient A and active ingredient B, active ingredient A is polyoxin, and active ingredient B is a triazole sulfonamide fungicide; wherein the triazole sulfonamide fungicide is selected from any one or two of cyfluthrin and epoxiconazole.

[0013] In other embodiments, the present invention provides a composition comprising active ingredient A and active ingredient B, wherein active ingredient A is polyoxin, and active ingredient B is any one of cyfluthrin or epoxiconazole.

[0014] In some further embodiments, the present invention provides a composition, wherein the active ingredients of the composition are active ingredient A and active ingredient B, active ingredient A is polyoxin, and active ingredient B is any one of cyfluthrin or epoxiconazole.

[0015] Furthermore, in the composition, the mass ratio of active ingredient A to active ingredient B is (1-100):(100-1);

[0016] Furthermore, in the composition, the mass ratio of active ingredient A to active ingredient B is (1-40):(40-1);

[0017] Preferably, in the composition, the mass ratio of active ingredient A to active ingredient B is (1-30): (30-1);

[0018] More preferably, in the composition, the mass ratio of active ingredient A to active ingredient B is (1-20):(20-1);

[0019] Further preferably, in the composition, the mass ratio of active ingredient A to active ingredient B is (1-10):(10-1);

[0020] More preferably, in the composition, the mass ratio of active ingredient A to active ingredient B is (1-5):(10-1);

[0021] More preferably, in the composition, the mass ratio of active ingredient A to active ingredient B is (1-5):(5-1).

[0022] Furthermore, based on the total content of the composition being 100% by mass, the sum of the contents of active ingredient A and active ingredient B in the composition is 1 to 95% by mass;

[0023] Furthermore, the sum of the contents of active ingredient A and active ingredient B in the composition is 1 to 80% by mass;

[0024] Preferably, the sum of the contents of active ingredient A and active ingredient B in the composition is 1 to 60% by mass;

[0025] More preferably, the total content of active ingredient A and active ingredient B in the composition is 5 to 60% by mass.

[0026] Furthermore, in the composition, the mass ratio of polyoxin and cyfluthrin is (1-40): (40-1);

[0027] Preferably, in the composition, the mass ratio of polyoxin and cyfluthrin is (1-30): (30-1);

[0028] More preferably, in the composition, the mass ratio of polyoxin to cyfluthrin is (1-20): (20-1);

[0029] Further preferably, in the composition, the mass ratio of polyoxin and cyfluthrin is (1-10): (10-1);

[0030] More preferably, in the composition, the mass ratio of polyoxin to cyfluthrin is (1-5):(5-1).

[0031] Specifically, in the composition, the mass ratio of polyoxin to cyfluthrin is 30:1, 20:1, 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:10, 1:20 or 1:30.

[0032] Furthermore, the sum of the contents of polyoxin and cyfluthrin in the composition is 1 to 95% by mass;

[0033] Furthermore, the sum of the contents of polyoxin and cyfluthrin in the composition is 1 to 80% by mass;

[0034] Preferably, the sum of the contents of polyoxin and cyfluthrin in the composition is 1 to 60% by mass;

[0035] More preferably, the total content of polyoxin and cyfluthrin in the composition is 5 to 60% by mass.

[0036] Specifically, the sum of the contents of polyoxin and cyfluthrin in the composition is 6 mass%, 10.5 mass%, 12 mass%, 31 mass%, 42 mass% or 60 mass%, etc.

[0037] Furthermore, in the composition, the mass ratio of polyoxin and epoxiconazole is (1-40): (40-1);

[0038] Preferably, in the composition, the mass ratio of polyoxin and epoxiconazole is (1-30): (30-1);

[0039] More preferably, in the composition, the mass ratio of polyoxin to epoxiconazole is (1-20): (20-1);

[0040] Further preferably, in the composition, the mass ratio of polyoxin to epoxiconazole is (1-10): (10-1);

[0041] More preferably, in the composition, the mass ratio of polyoxin to epoxiconazole is (1-5): (10-1);

[0042] More preferably, in the composition, the mass ratio of polyoxin to epoxiconazole is (1-5):(5-1).

[0043] Specifically, the mass ratio of polyoxin to bromocycline in the composition is 30:1, 20:1, 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:10, 1:20 or 1:30.

[0044] Furthermore, the sum of the contents of polyoxin and epoxiconazole in the composition is 1 to 95% by mass;

[0045] Furthermore, the sum of the contents of polyoxin and epoxiconazole in the composition is 1 to 80% by mass;

[0046] Preferably, the sum of the contents of polyoxin and epoxiconazole in the composition is 1 to 60% by mass;

[0047] More preferably, the sum of the contents of polyoxin and epoxiconazole in the composition is 5 to 60% by mass;

[0048] More preferably, the total content of polyoxin and epoxiconazole in the composition is 5 to 55% by mass.

[0049] Specifically, the sum of the contents of polyoxin and epoxiconazole in the composition is 6 mass %, 6.2 mass %, 20 mass %, 31 mass %, 48 mass % or 55 mass %, etc.

[0050] In a second aspect, the present invention provides a preparation, which is prepared from the composition of the present invention.

[0051] Furthermore, the dosage form of the preparation includes any one of aqueous solution, soluble solution, wettable powder, water-dispersible granule, suspension, dispersible oil suspension, emulsifiable concentrate, water emulsion, microemulsion, and microcapsule suspension.

[0052] Preferably, the formulation comprises wettable powder, water dispersible granules or suspension concentrate.

[0053] Furthermore, in the preparation, the mass ratio of active ingredient A to active ingredient B is (1-100):(100-1);

[0054] Furthermore, in the preparation, the mass ratio of active ingredient A to active ingredient B is (1-40):(40-1);

[0055] Preferably, in the preparation, the mass ratio of active ingredient A to active ingredient B is (1-30):(30-1);

[0056] More preferably, in the preparation, the mass ratio of active ingredient A to active ingredient B is (1-20):(20-1);

[0057] Further preferably, in the preparation, the mass ratio of active ingredient A to active ingredient B is (1-10):(10-1);

[0058] More preferably, in the preparation, the mass ratio of active ingredient A to active ingredient B is (1-5):(10-1);

[0059] More preferably, in the preparation, the mass ratio of active ingredient A to active ingredient B is (1-5):(5-1).

[0060] Furthermore, based on the total content of the preparation being 100% by mass, the sum of the contents of active ingredient A and active ingredient B in the preparation is 1 to 95% by mass;

[0061] Furthermore, the sum of the contents of active ingredient A and active ingredient B in the preparation is 1 to 80% by mass;

[0062] Preferably, the sum of the contents of active ingredient A and active ingredient B in the preparation is 1 to 60% by mass;

[0063] More preferably, the total content of active ingredient A and active ingredient B in the preparation is 5 to 60% by mass.

[0064] Furthermore, in the preparation, the mass ratio of polyoxin and cyfluthrin is (1-40): (40-1);

[0065] Preferably, in the preparation, the mass ratio of polyoxin and cyfluthrin is (1-30): (30-1);

[0066] More preferably, in the preparation, the mass ratio of polyoxin to cyfluthrin is (1-20): (20-1);

[0067] Further preferably, in the preparation, the mass ratio of polyoxin and cyfluthrin is (1-10): (10-1);

[0068] More preferably, in the preparation, the mass ratio of polyoxin to cyfluthrin is (1-5):(5-1).

[0069] Specifically, in the preparation, the mass ratio of polyoxin to cyfluthrin is 30:1, 20:1, 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:10, 1:20 or 1:30.

[0070] Furthermore, the sum of the contents of polyoxin and cyfluthrin in the preparation is 1 to 95% by mass;

[0071] Furthermore, the sum of the contents of polyoxin and cyfluthrin in the preparation is 1 to 80% by mass;

[0072] Preferably, the sum of the contents of polyoxin and cyfluthrin in the preparation is 1 to 60% by mass;

[0073] More preferably, the total content of polyoxin and cyfluthrin in the preparation is 5 to 60% by mass.

[0074] Specifically, the sum of the contents of polyoxin and cyfluthrin in the preparation is 6 mass %, 10.5 mass %, 12 mass %, 31 mass %, 42 mass % or 60 mass %, etc.

[0075] Furthermore, in the preparation, the mass ratio of polyoxin and epoxiconazole is (1-40): (40-1);

[0076] Preferably, in the preparation, the mass ratio of polyoxin and epoxiconazole is (1-30): (30-1);

[0077] More preferably, in the preparation, the mass ratio of polyoxin and epoxiconazole is (1-20): (20-1);

[0078] Further preferably, in the preparation, the mass ratio of polyoxin and epoxiconazole is (1-10): (10-1);

[0079] More preferably, in the preparation, the mass ratio of polyoxin to epoxiconazole is (1-5): (10-1);

[0080] More preferably, in the preparation, the mass ratio of polyoxin to epoxiconazole is (1-5):(5-1).

[0081] Specifically, the mass ratio of polyoxin to bromocycline in the preparation is 30:1, 20:1, 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:10, 1:20 or 1:30.

[0082] Furthermore, the sum of the contents of polyoxin and epoxiconazole in the preparation is 1 to 95% by mass;

[0083] Furthermore, the sum of the contents of polyoxin and epoxiconazole in the preparation is 1 to 80% by mass;

[0084] Preferably, the sum of the contents of polyoxin and epoxiconazole in the preparation is 1 to 60% by mass;

[0085] More preferably, the sum of the contents of polyoxin and epoxiconazole in the preparation is 5 to 60% by mass.

[0086] More preferably, the sum of the contents of polyoxin and epoxiconazole in the preparation is 5 to 55% by mass.

[0087] Specifically, the sum of the contents of polyoxin and epoxiconazole in the preparation is 6 mass%, 6.2 mass%, 20 mass%, 31 mass%, 48 mass% or 55 mass%, etc.

[0088] In a third aspect, the present invention provides use of the composition and / or formulation of the present invention in preventing and controlling plant diseases.

[0089] Wherein, the plants are selected from one or more of cereals, vegetables, fruits and flowers.

[0090] The plant diseases are selected from Oomycete diseases such as late blight, downy mildew, and peronospora.

[0091] Preferably, the plant disease is selected from potato late blight, tomato late blight, pepper blight, watermelon blight, ginseng blight, cucumber downy mildew, grape downy mildew, cabbage downy mildew and / or litchi downy mildew, more preferably cucumber downy mildew, potato late blight and / or tomato late blight.

[0092] In a fourth aspect, the present invention provides a method for controlling plant diseases, comprising applying an effective amount of the composition and / or formulation of the present invention to plants and / or plant parts.

[0093] The compositions and formulations provided by the present invention can produce a strong synergistic effect, surprisingly maintaining the same beneficial effects as higher application rates of polyoxin, cyfluthrin, and epoxiconazole while reducing their application rates. Therefore, the fungicidal compositions and formulations provided by the present invention substantially enhance disease control efficacy while significantly improving safety.

[0094] In addition to the synergistic fungicidal activity, the fungicidal composition and its preparation provided by the present invention have strong synergistic effects, such as: the fungicidal spectrum is broadened to other plant pathogens (such as resistant strains of plant diseases); the application rate of the active compound is reduced; favorable properties in the preparation or use process (such as grinding, screening, emulsification, dissolution or dispersion) are increased; enhanced storage stability and light stability; favorable residue formation; improved toxicological and ecotoxicological properties; improved plant properties, such as better growth, increased harvest yield, more developed root system, larger leaf area, greener leaves, stronger shoots, less seed requirement, lower phytotoxicity, mobilization of plant defense system, and good compatibility with plants.

[0095] Therefore, using the compositions and formulations provided by the present invention is very helpful in maintaining plant health while also ensuring quality and yield. Furthermore, the fungicidal compositions and formulations provided by the present invention can help enhance systemic action. Similarly, the fungicidal compositions and formulations provided by the present invention have a longer lasting effect.

[0096] The term "include" or "comprising" is an open expression, that is, including the contents specified in the present invention, but not excluding other contents.

[0097] The term "effective amount" refers to the total amount of each active ingredient sufficient to show the desired control effect. This amount varies within a wide range depending on the fungus to be controlled, plant species, climatic conditions and the compounds contained in the composition of the present invention.

[0098] The term "preventing" means prevention and / or treatment.

[0099] The composition or preparation of the present invention, in addition to the above-mentioned active ingredients, usually also contains adjuvants commonly used in pesticides, such as wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists, carriers, etc., which can be appropriately added according to the needs of the preparation and can vary according to different situations, and are not particularly limited.

[0100] Adjuvants are auxiliary substances added during the processing or use of pesticide formulations to improve the physical and chemical properties of the pesticides. The adjuvants themselves have basically no biological activity, but can affect the control effect.

[0101] The wetting agent is selected from one or more of alkylphenol polyoxyethylene ethers (such as nonylphenol polyoxyethylene ether, octylphenol polyoxyethylene ether, dodecylphenol ether, dinonylphenol ether and mixed alkylphenol ether, etc.), fatty alcohol polyoxyethylene ether, fatty acid or fatty acid ester sulfate, alkylnaphthalene sulfonate (such as sodium dibutylnaphthalene sulfonate, sodium isopropylnaphthalene sulfonate), alkylbenzene sulfonate (such as sodium dodecylbenzene sulfonate, calcium dodecylbenzene sulfonate), sodium lauryl sulfate (SDS), succinate sulfonate, alkyl sulfate and lignin sulfonate (such as sodium lignin sulfonate, calcium lignin sulfonate). Typical representatives of suitable wetting agents are especially OTB (dioctyl sulfosuccinate), EFW (alkylated naphthalenesulfonate), BX (alkylated naphthalenesulfonate), MT 804 (alkylated naphthalenesulfonate), AL / 304 (EOPO block copolymer), BC / 10 (alkylphenol polyoxyethylene ether).

[0102] The dispersant is selected from one or more of naphthalenesulfonate, naphthalenesulfonate formaldehyde condensate, ligninsulfonate, alkylbenzenesulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, high molecular weight polycarboxylate, alkylbenzene polyoxyethylene ether, ethylene oxide propylene oxide block polyether and organic phosphate. For wettable powder preparations, the most common dispersant is sodium lignosulphonate. For suspension concentrates, polyelectrolytes such as sodium naphthalene sulfonate formaldehyde condensate are used to obtain very good adsorption and stabilization. Tristyrylphenol ethoxylate phosphate ester is also used. Typical representatives of suitable dispersants include Ultrazine NA, GY-D06, Antarox B / 848, Geropon Ultrasperse, Supragil NSR, JR-P, Soprophor SC, Morwet D425 or EthylanNS-500LQ.

[0103] The emulsifier is selected from one or more of fatty alcohol polyoxyethylene ethers, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene polyoxypropylene ethers, fatty amines, ethylene oxide adducts of fatty amides, fatty acid polyoxyethylene esters, castor oil ethylene oxide adducts and derivatives thereof, rosin acid ethylene oxide adducts and the like, polyol fatty acids and ethylene oxide adducts thereof, and polyoxyethylene polyoxypropylene block copolymers. Typical examples of suitable dispersants include Nongru 500#, Nongru 601#, Nongru 602#, Nongru 700#, Nongru 1601#, Nongru 1602#, Antarox AL / 304, or Ethylan 992.

[0104] The thickener is selected from one or more of white carbon black, kaolin, bentonite, magnesium aluminum silicate, xanthan gum, gum arabic, polyacrylic acid and sodium carboxymethyl cellulose.

[0105] The disintegrant is selected from one or more of urea, modified starch, bentonite, aluminum chloride, ammonium sulfate, succinic acid, sodium bicarbonate, sodium chloride and the like.

[0106] The antifreeze agent is selected from one or more of glycerol, ethylene glycol, propylene glycol and the like.

[0107] The defoaming agent is selected from one or more of organic silicone defoaming agents, C8-10 fatty alcohol defoaming agents, polyurea and polyethylene glycol fatty acid ester.

[0108] The solvent is one or more of toluene, xylene, cyclohexanone, aromatic solvent, methanol, ethyl acetate, soybean oil, epoxidized soybean oil, castor oil, methyl oleate, rapeseed oil and N-octyl pyrrolidone.

[0109] The preservative is selected from one or more of sodium benzoate, benzoic acid, potassium sorbate, and kason.

[0110] The stabilizer is selected from one or more of phosphoric acid, pyrophosphoric acid, phthalic acid, benzoic acid, triphenyl phosphite, acetic anhydride, epichlorohydrin, resorcinol and sodium citrate.

[0111] The synergist itself has no biological activity, but can inhibit the detoxification enzymes in organisms. When mixed with certain pesticides, it can significantly increase the toxicity and efficacy of the pesticides, such as one or more of synergistic phosphorus and piperonyl butoxide.

[0112] The carrier is selected from one or more of kaolin, bentonite, light calcium carbonate, white carbon black, clay, talc, chalk, quartz, attapulgite, montmorillonite, diatomaceous earth, corn starch, silicon dioxide, aluminum oxide, natural or artificial silicates, resins, waxes, solid fertilizers, water, alcohols (especially butanol), organic solvents, mineral oils, vegetable oils and their derivatives.

[0113] If appropriate, further additional components may also be present, such as protective colloids, binders, adhesives, thixotropic substances, penetrants, chelating agents, complex formers, dyes, etc. In general, the active compounds can be combined with any solid or liquid additives customary for formulation purposes.

[0114] The compositions provided by the invention can be used as such or, depending on their physical and / or chemical properties, in the form of their formulations or use forms prepared therefrom, such as aerosols, capsule suspensions, cold mist concentrates, hot mist concentrates, encapsulated granules, fine granules, flowable concentrates for treating seeds, ready-to-use solutions, dustable powders, emulsifiable concentrates, oil-in-water emulsions, water-in-oil emulsions, macrogranules, microgranules, oil-dispersible powders, oil-soluble flowable concentrates, oil-soluble liquids, foams, pastes, insecticide-coated seeds, suspension concentrates, suspoemulsion concentrates, soluble concentrates, suspensions, wettable powders, soluble powders, dusts and granules, water-soluble granules or tablets, water-soluble powders for treating seeds, wettable powders impregnated with the active compound, microcapsules in natural products and synthetic substances and polymeric substances and seed coating materials, and ULV cold mist and hot mist formulations.

[0115] Typical formulations are soluble concentrates, dispersible oil suspensions, microcapsule suspensions, dusts, wettable powders, microcapsules, water-dispersible granules, granules, aqueous solutions, suspensions, oil suspensions, emulsifiable concentrates, microemulsions, emulsions in water, ultra-low volume sprays, seed coatings or smoke agents.

[0116] Powder (DP) is a mixture of active substances added with a certain amount of inert powder, such as clay, kaolin, talc, etc., which is mechanically processed into a powder with a particle diameter of less than 100 microns.

[0117] Wettable powders (WP) are mixtures of a composition with a suitable surfactant and an inert substance (kaolin powder) in a specific ratio. Wettable powders are preparations that can be evenly dispersed in water. In addition to the active ingredient and the inert substance (kaolin powder), they also contain a certain amount of anionic or nonionic surfactants (dispersants, wetting agents).

[0118] Microcapsules (MC) are made by first encapsulating the active ingredient in a binder, film-forming agent, etc. to form a tiny capsule preparation, which is then processed into the required dosage form. They are divided into microcapsule suspensions (CS), microcapsulated granules (CG), and microcapsulated dry flowables (CDF).

[0119] Water-dispersible granules (WDG) are a mixture of a composition, a suitable surfactant, and an inert material (kaolin) powder in a specific ratio. The powder is jet-milled to the required fineness and then mixed multiple times using a twin-screw mixer and a plowshare mixer to achieve uniformity. The product is then manufactured using conventional methods such as fluidized bed granulation, spray granulation, or pan granulation.

[0120] Granules (GR) are a mixture of a composition with a suitable surfactant, diluent, and inert material (kaolin powder) in a specific proportion. Granulation is achieved using a granulator, such as fluidized bed granulation, where a binder solution is sprayed onto the powder while the powder is kept in a fluid state to cause agglomeration. This method allows the mixing, kneading, granulation, drying, and classification processes to be completed in a single, closed unit within a short time.

[0121] Aqueous solution (AS) is a liquid prepared directly with water, utilizing the property of certain original drugs that can dissolve in water without decomposing.

[0122] Suspension concentrate (SC) is prepared by mixing a composition with a suitable surfactant, water or organic solvent in a certain proportion, grinding it uniformly with a colloid mill, and then grinding it to a certain fineness with a sand mill.

[0123] Oil suspension concentrate (OD / OF) refers to a composition dispersed in a non-aqueous medium (mineral oil, vegetable oil and its derivatives, etc.) in the form of tiny particles. With the help of a suitable surfactant (such as an emulsifier, dispersant, etc.), the drug is evenly dispersed in the oil phase by grinding with a sand mill.

[0124] Emulsifiable concentrate (EC) is prepared by dissolving a composition in an organic solvent such as benzene, toluene, xylene, or cyclohexanone in a certain proportion and adding a certain amount of anionic or nonionic surfactant (emulsifier) to the mixture.

[0125] Microemulsions (MEs) typically consist of a liquid pesticide, a surfactant (emulsifier), water, and a stabilizer. They are characterized by their water-based medium, containing little or no organic solvents. The droplet size in a microemulsion typically ranges from a few nanometers to tens of nanometers, less than a quarter of the wavelength of visible light.

[0126] Emulsion in water (EW) is a pesticide formulation in which a water-insoluble original drug liquid or a solution obtained by dissolving the original drug in a water-insoluble organic solvent is dispersed in water.

[0127] Ultra-low volume spray refers to the liquid medicine sprayed onto the target crops in extremely fine droplets and extremely low dosage. It is a special formulation for ultra-low volume spray application.

[0128] Seed coating agent (SD) is made by grinding and mixing active ingredients and adjuvants. It can be coated on the surface of seeds directly or after dilution to form a protective film with a certain strength and permeability.

[0129] Smoke agent (FU), also known as aerosol, is a kind of agent that mixes active ingredients with combustible substances, burns them, vaporizes the pesticides and condenses them into smoke particles, or directly disperses the pesticides into smoke particles.

[0130] Soluble solutions (SL) are liquid preparations in which the active ingredient is dissolved in a water-soluble organic solvent and can be diluted with water to become transparent or translucent when used.

[0131] Regarding the meaning of various adjuvants and various preparations, differences in expression may lead to differences in understanding. It should be understood that various adjuvants and preparations disclosed in the art are all within the scope of the present invention, such as those described in "Pesticide Formulation Processing Technology", Luo Yanping, Song Weiwei, Chemical Industry Press, 2015; "Pesticide Formulation Science", Wang Kaiyun, China Agriculture Press, 2009; "Modern Pesticide Formulation Processing Technology Series", Liu Guangwen, Chemical Industry Press, 2018; "Pesticide Formulations and Preparations and Usage Methods", Tu Yuqin, Golden Shield Press, 2008; "Chinese Pesticides", China Pesticide Industry Association; etc. The preparation methods of various preparations include but are by no means limited to those described in the present invention.

[0132] The above-mentioned formulations can be prepared in a manner known per se, for example by mixing the active compound or active compound combination with at least one additive. Suitable additives are all customary formulation auxiliaries, for example organic solvents, extenders, solvents or diluents, solid carriers and fillers, surfactants (for example adjuvants, emulsifiers, dispersants, protective colloids, wetting agents and tackifiers), dispersants and / or binders or fixatives, preservatives, dyes and pigments, defoamers, inorganic and organic thickeners, hydrophobic agents, and, if appropriate, driers and UV stabilizers, gibberellins, as well as water and other processing aids. In each case, depending on the type of formulation to be prepared, further processing steps, for example wet grinding, dry grinding or granulation, may be necessary.

[0133] The compositions according to the invention encompass both ready-to-use compositions, which can be applied to plants or seeds using suitable devices, and commercial concentrates, which must be diluted before use.

[0134] According to the present invention, all plants and plant parts can be processed.Plant refers to all plants and plant populations, for example, want and unwanted wild plants, cultivars and plant varieties (no matter whether protected by plant variety right or plant breeder's right).Cultivars and plant varieties can be by conventional breeding and breeding methods (assisted or supplemented by one or more biotechnology methods, for example, by using double haploid, protoplast fusion, random mutagenesis and directed mutagenesis, molecular markers or genetic markers), or the plants obtained by the method for bioengineering and genetic engineering.Plant parts refer to all above-ground and underground parts and organs of plants, wherein list for example bud, leaf, flower and root, for example leaf, needle, stem, branch, flower, fruiting body, fruit and seed and root, bulb and rhizome.Crops and asexual and sexual propagation materials, for example cuttings, bulb, rhizome, barberry and seed also belong to plant parts.

[0135] The treatment of plants and plant parts with the fungicidal compositions and formulations provided by the present invention can be carried out directly or in their surroundings, habitats or storage areas using conventional treatment methods, for example by dipping, spraying, misting, irrigation, evaporation, dusting, misting, broadcasting, foaming, smearing, coating, watering (irrigation), dripping, and in the case of propagation materials, in particular seeds, by incrustation, coating with one or more coatings, etc., with powders for treating dry seeds, with solutions for treating seeds, with slurries for treating with water-soluble powders. The compositions or formulations can also be injected into the soil.

[0136] Among the plants which can be protected by the method according to the invention, there may be mentioned the major crop plants, such as maize, soybeans, potatoes, cotton, rapeseed, rice, wheat, sugar beets, sugar cane, oats, rye, barley, millet, triticale, flax, vines and various fruits and vegetables from various plant taxa, horticultural and forest crops; ornamental plants; and genetically modified homologs of these crops.

[0137] The present invention also includes methods for treating seeds using the fungicidal compositions or formulations of the present invention. The compositions of the present invention can be applied directly, i.e., without other components and without dilution. Generally, it is preferred to apply the compositions of the present invention to the seeds in a suitable formulation. Suitable formulations and methods for treating seeds are conventional in the art. The compositions of the present invention can be converted into conventional seed dressing formulations, such as solutions, emulsions, suspensions, powders, foams, slurries, or other coating materials for seeds, as well as ULV formulations.

[0138] Depending on the plant species or plant cultivars, their location and growth conditions (soil, climate, vegetative period, nutrition), the treatment according to the invention may also result in superadditive ("synergistic") effects. For example, the following effects exceeding actual expectations may be achieved: reduced application rates and / or a broadened spectrum of activity and / or increased activity of the fungicidal compositions and formulations provided by the invention, better plant growth, increased tolerance to high or low temperatures, increased tolerance to drought or water or soil salinity, improved flowering quality, easier harvesting, accelerated ripening, higher harvest yield, larger fruits, taller plant height, greener leaf color, earlier flowering, higher quality and / or higher nutritional value of the harvested product, higher sugar concentration in the fruit, better storage stability and / or better processability of the harvested product.

[0139] Compared with the prior art, by rationally compounding and using pesticides, the beneficial effects of the fungicidal composition / preparation of the present invention include at least one of the following: (1) a strong synergistic effect, (2) the ability to delay the generation and development of drug resistance, (3) improved safety for crops, (4) reduced drug and labor costs, and (5) reduced environmental pollution caused by excessive use of pesticides. DETAILED DESCRIPTION

[0140] The present invention is further described in detail below through specific examples, but the present invention is by no means limited to the following embodiments. All equivalent transformations based on the technical solution of this application fall within the scope of protection of the present invention. If the specific conditions are not specified in the examples, they shall be carried out according to conventional conditions or the conditions recommended by the manufacturer. Both cyfluthrin and epoxiconazole are homemade. Cyfluthrin is prepared by the preparation method of Example 12 in application number PCT / CN2019 / 108170; epoxiconazole is prepared by the preparation method of Example 8 in application number PCT / CN2018 / 081979; polyoxin is purchased from Shaanxi Maikeluo Biotechnology Co., Ltd.; other reagents or instruments used without indicating the manufacturer are conventional products that can be purchased commercially or prepared according to existing technologies.

[0141] Synthesis and characterization of cyfluthrin:

[0142]

[0143] N 3 -(3-(trifluoromethyl)benzyl)-N 1 ,N 1 -Dimethyl-1H-1,2,4-triazole-1,3-disulfonamide (0.41 g, 1.0 mmol), potassium carbonate (0.28 g, 2.0 mmol) and N,N-dimethylformamide (10 mL) were added to a 100 mL three-necked flask, and then bromocyclopropane (0.24 g, 2.0 mmol) was slowly added to the reaction system and reacted at room temperature for 8 hours; water (20 mL) was added to quench the mixture, and the mixture was extracted with ethyl acetate (15 mL×3). The organic phase was washed with saturated brine, dried over anhydrous magnesium sulfate, and filtered. The filtrate was freed from the solvent under reduced pressure on a rotary evaporator and purified by column chromatography (ethyl acetate / petroleum ether (v / v) = 1 / 3) to obtain 0.24 g of a white solid with a yield of 53%.

[0144] 1 H NMR (400MHz, CDCl3): δ (ppm) 8.76 (s, 1H), 7.49-7.16 (m, 4H), 3.82 (s, 2H), 2.67 (s, 6H), 1.36-1.32 (m, 1H), 0.71-0.68 (m, 2H), 0.22-0.19 (m, 2H).

[0145] LC-MS: (M+1) m / z=454.0.

[0146] Synthesis and characterization of epoxiconazole:

[0147]

[0148] Potassium carbonate (0.276 g, 2.0 mmol), N3-cyclopropyl-N 1 ,N 1 1-Dimethyl-1H-1,2,4-triazole-1,3-disulfonamide (0.40 g, 1.36 mmol), 1-bromo-3-(bromomethyl)benzene (0.48 g, 2.0 mmol), and N,N-dimethylformamide (15 ml) were added to a 50 mL three-necked flask and stirred at room temperature for 12 hours. Water (30 mL) was added and the mixture was extracted with ethyl acetate (20 mL x 3). The organic phase was washed with saturated brine (10 ml), dried over magnesium sulfate, and filtered. The filtrate was freed from the solvent under reduced pressure using a rotary evaporator. The residue was purified by column chromatography (ethyl acetate / petroleum ether (v / v) = 1 / 5) to obtain 0.287 g of a white solid in a 45% yield.

[0149] 1 H NMR (400MHz, CDCl3) δ (ppm): 8.63 (s, 1H), 7.52 (s, 1H), 7.44 (d, J = 7.9Hz, 1H), 7.33 (d, J = 7.7Hz, 1H), 7. 21(t,J=7.8Hz,1H),4.54(s,2H),3.08(s,6H),2.58-2.53(m,1H),0.87-0.83(m,2H),0.77-0.73(m,2H).

[0150] LC-MS: (M+1) m / z=464.0.

[0151] Indoor bio-testing

[0152] The mycelial growth rate method was used to determine the inhibitory effect of different agents on the growth of potato late blight (Phytophthorainfestans (Mont.) de Bary, strain source: Professor Shan Weixing's laboratory at Northwest Agriculture and Forestry University). The EC value of each agent against the pathogen was calculated. 50 value.

[0153] Rye culture medium (RSA): 60 g rye; 20 g sucrose; 15 g agar powder. Soak the rye in 200 mL of distilled water for 36 hours. Filter through two layers of gauze and save the filtrate. Stir the rye for 2 minutes, add 400 mL of distilled water, and incubate in a 50°C waterbath for 3 hours. Filter through four layers of gauze. Combine the two filtrates, add sucrose and agar powder, and dilute to 1000 mL. Sterilize by autoclaving at 121°C for 15 minutes.

[0154] A single dose or each combination (see Table 1 and Table 2) was dissolved in DMSO and diluted with 0.1 wt% Tween 80 water to an appropriate concentration gradient (5 mg / L, 2.5 mg / L, 1.25 mg / L, 0.625 mg / L, 0.3125 mg / L, 0.15625 mg / L) for later use.

[0155] In a clean bench, add 5 mL of sterilized sample (sterilized by UV sterilization) to 45 mL of RSA medium (cooled to 45-50°C), mix thoroughly, pour onto a plate (90 mm diameter), and cool to a gradient of final drug concentrations. Use RSA medium supplemented with an equal amount of sterile Tween water as a control. Repeat three times.

[0156] Pick a late blight fungus block (6 mm in diameter) and place it upside down in the center of the inoculum plate containing the virus. Incubate in a constant temperature incubator at 20°C for 10 days. Measure the colony diameter using the cross-hatch method and calculate the relative inhibition rate according to formulas (2.1) and (2.2).

[0157] Colony expansion diameter (mm) = average colony diameter (mm) - 6 (mm) (2.1)

[0158]

[0159] According to the concentration of each drug and the corresponding relative inhibition rate, the corresponding regression analysis was performed to calculate the EC of each drug. 50 Values and 95% confidence intervals.

[0160] The biostatistical probability value can be obtained by looking up the relative inhibition rate table. Using the logarithm of the concentration as the horizontal axis, according to the "Biostatistical Probability Value Conversion Table", the inhibition percentage is converted into the biostatistical probability value as the vertical axis, and the toxicity regression equation is drawn. The mass concentration when the probability value is 5 is obtained by using the equation, which is the EC 50 (Millot M, Girardot M, Dutreix L, et al.. Antifungal and anti-biofilm activities of acetone lichen extracts against Candida albicans. [J]. Molecules, 2017, 22(4): 651.).

[0161] The co-toxicity coefficient calculation method introduced by Sun Yunpei was used to calculate the co-toxicity coefficient (CTC) of the mixture and determine the synergistic effect of the mixture.

[0162] Take a single agent in the mixture as the standard agent (usually EC 50 The lower one), calculate:

[0163] Single-dose toxicity index = standard drug EC 50 / A single dose of EC 50 ×100

[0164] Theoretical toxicity index (TTI) = toxicity index of single agent A × the proportion of single agent A in the mixture + toxicity index of single agent B × the proportion of single agent B in the mixture

[0165] Measured Toxicity Index (ATI) = EC of a standard single dose 50 EC value / mixture 50 value × 100

[0166] Co-toxicity coefficient (CTC) = measured toxicity index / theoretical toxicity index × 100

[0167] When CTC is greater than 120, the mixture has synergistic effects, when CTC is less than 80, it is antagonistic, and when CTC is between 80-120, it is additive.

[0168] Table 1: Determination of the co-toxicity coefficient of different ratios of polyoxin and cyfluthrin against potato late blight

[0169]

[0170]

[0171] The results in Table 1 show that when polyoxin and cyfluthrin are combined in a mass ratio of (1-30):(30-1), the cotoxicity coefficient against potato late blight is greater than 120, demonstrating a significant synergistic effect. This suggests that the combination of polyoxin and cyfluthrin is an ideal agent for controlling potato late blight.

[0172] Table 2: Determination of the co-toxicity coefficient of different ratios of polyoxin and bromoepiconazole against potato late blight

[0173]

[0174]

[0175] The results in Table 2 show that when polyoxin and epoxiconazole are combined in a mass ratio of (1-30):(30-1), the cotoxicity coefficient against potato late blight is greater than 120, demonstrating a significant synergistic effect. This suggests that the combination of polyoxin and epoxiconazole is an ideal agent for controlling potato late blight.

[0176] Preparation Example 1: 60% polyoxin-cyfluthrin wettable powder (1:5)

[0177] 10% polyoxindole, 50% cyfluthrin, 7% polycarboxylate (trade name: Geropon Ultrasperse, Solvay), 2% sulfonate wetting agent (trade name: Morwet EFW, Akzo Nobel) and corn starch (Aladdin reagent) supplemented to 100% were pre-crushed and mixed uniformly, and then pulverized in a jet mill (Kunshan Unak Machinery Co., Ltd.) to prepare a polyoxindole-cyfluthrin wettable powder with an active ingredient mass content of 60%.

[0178] Preparation Example 2: 10.5% polyoxin-cyfluthrin wettable powder (20:1)

[0179] 10% polyoxindole, 0.5% cyfluthrin, 6% naphthalenesulfonate formaldehyde condensate (trade name: Morwet D425, Akzo Nobel), 2% sodium lauryl sulfate (Aladdin reagent), 5% white carbon black (Aladdin reagent) and bentonite (Hangzhou Zuotu New Materials Co., Ltd.) supplemented to 100% were pre-crushed and mixed uniformly, and then pulverized by a jet mill (Kunshan Unak Machinery Co., Ltd.) to prepare a polyoxindole-cyfluthrin wettable powder with an active ingredient content of 10.5% by mass.

[0180] Preparation Example 3: 55% polyoxin-bromocycline wettable powder (1:10)

[0181] 5% polyoxindole, 50% bromoepiconazole, 8% polycarboxylate (trade name: GY-D06, Beijing Guangyuan Yino Chemical Co., Ltd.), 3% sodium lauryl sulfate (Aladdin reagent) and bentonite (Hangzhou Zuotu New Materials Co., Ltd.) supplemented to 100% were pre-crushed and mixed evenly, and then pulverized by a jet mill (Kunshan Unak Machinery Co., Ltd.) to obtain a polyoxindole-bromoepiconazole wettable powder with an active ingredient content of 55% by mass.

[0182] Preparation Example 4: 20% polyoxin-bromocycline wettable powder (1:1)

[0183] 10% polyoxin, 10% epoxiconazole, 7% lignin sulfonate (trade name: Ultrazine NA, Borregaard Industries Co., Ltd.), 2% sodium lauryl sulfate (Aladdin reagent), 5% white carbon black (Aladdin reagent) and kaolin (Hangzhou Zuotu New Materials Co., Ltd.) to make up to 100% were pre-crushed and mixed uniformly, and then pulverized by a jet mill (Kunshan Unak Machinery Co., Ltd.) to prepare a polyoxin and epoxiconazole wettable powder with an active ingredient content of 20% by mass.

[0184] Preparation Example 5: 31% polyoxin-cyfluthrin water dispersible granules (1:30)

[0185] 1% polyoxindole, 30% cyfluthrin, 7% polycarboxylate (trade name: Geropon Ultrasperse, Solvay), 2% sulfonate wetting agent (trade name: Morwet EFW, Akzo Nobel), 7% ammonium sulfate (Aladdin reagent), 5% white carbon black (Aladdin reagent) and bentonite (Hangzhou Zuotu New Materials Co., Ltd.) supplemented to 100% were pre-crushed and mixed, then pulverized by a jet mill (Kunshan Unak Machinery Co., Ltd.), granulated by a rotary granulator, dried, and sieved to obtain polyoxindole-cyfluthrin water dispersible granules with an active ingredient mass content of 31%.

[0186] Preparation Example 6: 42% polyoxin-cyfluthrin water dispersible granules (1:20)

[0187] 2% polyoxindole, 40% cyfluthrin, 8% naphthalenesulfonate formaldehyde condensate (trade name: Morwet D425, Akzo Nobel), 2% sodium lauryl sulfate (Aladdin reagent), 5% ammonium sulfate (Aladdin reagent), and kaolin (Hangzhou Zuotu New Materials Co., Ltd.) to 100% were pre-crushed and mixed, then pulverized by a jet mill (Kunshan Unak Machinery Co., Ltd.), granulated by a rotary granulator, dried, and sieved to obtain polyoxindole-cyfluthrin water-dispersible granules with an active ingredient content of 42% by mass.

[0188] Preparation Example 7: 48% polyoxin-bromoepiconazole water dispersible granules (1:5)

[0189] Polyoxin 8%, bromocycline 40%, polycarboxylate (trade name: 8% of Huntsman Corporation), 3% of sodium lauryl sulfate (Aladdin reagent), 8% of ammonium sulfate (Aladdin reagent) and kaolin (Hangzhou Zuotu New Materials Co., Ltd.) to make up to 100% were pre-crushed and mixed, then pulverized by a jet mill (Kunshan Unak Machinery Co., Ltd.), granulated by a rotary granulator, dried, and sieved to obtain polyoxin-bromoepiconazole water-dispersible granules with an active ingredient content of 48% by weight.

[0190] Preparation Example 8: 6.2% polyoxin-bromoepiconazole water dispersible granules (30:1)

[0191] 6% polyoxin, 0.2% epoxiconazole, 6% lignin sulfonate (Ultrazine NA, Borregaard Industries, Ltd.), 2% sulfonate wetting agent (trade name: Morwet EFW, Akzo Nobel), 5% ammonium sulfate (Aladdin reagent) and bentonite (Hangzhou Zuotu New Materials Co., Ltd.) supplemented to 100% were pre-crushed and mixed, then pulverized by a jet mill (Kunshan Unak Machinery Co., Ltd.), granulated by a rotary granulator, dried, and sieved to obtain polyoxin and epoxiconazole water-dispersible granules with an active ingredient content of 6.2% by mass.

[0192] Preparation Example 9: 6% polyoxin-cyfluthrin suspension (1:1)

[0193] (i) Preparation of pulverized slurry

[0194] Polyoxin 3%, cyfluthrin 3%, polycarboxylate (trade name: Huntsman Corporation) 3%, alkylphenol polyoxyethylene ether (trade name: Igepal BC / 10, Solvay) 1% and silicone defoamer (trade name: ETXP-40, Hangzhou Zuotu New Materials Co., Ltd.) 0.5% were fully dispersed in water supplemented to 80%, and then used. The glass beads were wet-ground using a sand mill (Shenyang Chemical Research Institute) to obtain 80% ground slurry.

[0195] (ii) Preparation of suspension concentrate

[0196] 0.3% of xanthan gum (Aladdin reagent), 0.8% of magnesium aluminum silicate (Hangzhou Zuotu New Materials Co., Ltd.), 5% of ethylene glycol (Aladdin reagent), and 0.5% of sodium benzoate (Aladdin reagent) were added to 80% of the crushed slurry and mixed evenly. Water was added to 100%, and high-speed shearing was performed to obtain a polyoxin-3-pyridoxine suspension with an active ingredient mass content of 6%.

[0197] Preparation Example 10: 12% polyoxin-cyfluthrin suspension (5:1)

[0198] (i) Preparation of pulverized slurry

[0199] 10% of polyoxin, 2% of cyfluthrin, 8% of naphthalenesulfonate formaldehyde condensate (trade name: Morwet D425, Akzo Nobel), 3% of EOPO block copolymer (trade name: Antarox AL / 304, Solvay) and 0.3% of organosilicon defoamer (trade name: ETXP-40, Hangzhou Zuotu New Materials Co., Ltd.) were fully dispersed in water supplemented to 80%. The glass beads were wet-ground using a sand mill (Shenyang Chemical Research Institute) to obtain 80% ground slurry.

[0200] (ii) Preparation of suspension concentrate

[0201] 0.2% of xanthan gum (Aladdin reagent), 0.4% of organic bentonite (Hangzhou Zuotu New Materials Co., Ltd.), 5% of propylene glycol (Aladdin reagent), and 0.5% of sodium benzoate (Aladdin reagent) were added to 80% of the crushed slurry and mixed evenly. Water was added to 100%, and high-speed shearing was performed to obtain a polyoxindazole-cyfluthrin suspension with an active ingredient mass content of 12%.

[0202] Preparation Example 11: 6% polyoxin-bromoepiconazole suspension (5:1)

[0203] (i) Preparation of pulverized slurry

[0204] Polyoxin 5%, bromocycline 1%, phosphate dispersant (trade name: Soprophor SC, Akzo Nobel) 3%, EO-PO block copolymer (trade name: Ethylan NS-500LQ, Akzo Nobel) 1%, sulfonate wetting agent (trade name: Morwet EFW, Akzo Nobel) 1% and silicone defoamer (trade name: ETXP-40, Hangzhou Zuotu New Materials Co., Ltd.) 0.4% were fully dispersed in water supplemented to 80%. The glass beads were wet-ground using a sand mill (Shenyang Chemical Research Institute) to obtain 80% ground slurry.

[0205] (ii) Preparation of suspension concentrate

[0206] 0.15% of xanthan gum (Aladdin reagent), 0.3% of organic bentonite (Hangzhou Zuotu New Materials Co., Ltd.), 4% of propylene glycol (Aladdin reagent), and 0.5% of sodium benzoate (Aladdin reagent) were added to 80% of the crushed slurry and mixed evenly. Water was added to 100%, and high-speed shearing was performed to obtain a polyoxin-1-bromoepiconazole suspension with an active ingredient mass content of 6%.

[0207] Preparation Example 12: 31% polyoxin-bromoepiconazole suspension (1:30)

[0208] (i) Preparation of pulverized slurry

[0209] Polyoxin 1%, bromocycline 30%, polycarboxylate ( 4% of a sulfonate wetting agent (trade name: Morwet EFW, Huntsman Corporation), 2% of a sulfonate wetting agent (trade name: Akzo Nobel) and 0.5% of a silicone defoamer (trade name: ETXP-40, Hangzhou Zuotu New Materials Co., Ltd.) were fully dispersed in water supplemented to 80%, and wet-ground using 0.6-0.8 mm φ glass beads in a sand mill (Shenyang Chemical Research Institute) to obtain 80% ground slurry.

[0210] (ii) Preparation of suspension concentrate

[0211] To 80% of the crushed slurry, 0.2% of xanthan gum (Aladdin reagent), 1.0% of magnesium aluminum silicate (Hangzhou Zuotu New Materials Co., Ltd.), 3% of ethylene glycol (Aladdin reagent), and 0.5% of sodium benzoate (Aladdin reagent) were added and mixed evenly. Water was added to 100%, and high-speed shearing was performed to obtain a polyoxinamide-bromoepiconazole suspension with an active ingredient mass content of 31%.

[0212] Control Example 1: 10% polyoxin wettable powder

[0213] 10% polyoxin, 6% polycarboxylate (trade name: Geropon Ultrasperse, Solvay), 2% sodium lauryl sulfate (Aladdin reagent) and kaolin (Hangzhou Zuotu New Materials Co., Ltd.) to make up to 100% were pre-crushed and mixed evenly, and then pulverized by a jet mill (Kunshan Unak Machinery Co., Ltd.) to prepare a polyoxin wettable powder with an active ingredient content of 10% by mass.

[0214] Control Example 2: 10% cyfluthrin wettable powder

[0215] 10% cyfluthrin, 5% polycarboxylate (trade name: Poerblox D-518, Nanjing Gutian Chemical Co., Ltd.), 2% sodium lauryl sulfate (Aladdin reagent), and bentonite (Hangzhou Zuotu New Materials Co., Ltd.) supplemented to 100% were pre-crushed and mixed uniformly, and then pulverized by a jet mill (Kunshan Unak Machinery Co., Ltd.) to prepare a cyfluthrin wettable powder with an active ingredient content of 10% by mass.

[0216] Control Example 3: 10% epoxiconazole wettable powder

[0217] 10% epoxiconazole, 6% polycarboxylate (trade name: Geropon TA72, Solvay), 2% sodium lauryl sulfate (Aladdin reagent), and diatomaceous earth (Hangzhou Zuotu New Materials Co., Ltd.) supplemented to 100% were pre-crushed and mixed uniformly, and then pulverized in a jet mill (Kunshan Unak Machinery Co., Ltd.) to prepare a epoxiconazole wettable powder with an active ingredient content of 10% by mass.

[0218] Field efficacy trials

[0219] Plot setting: Each treatment was repeated 4 times; the plot area was 30m 2 , randomized block arrangement.

[0220] Application time and method: Apply the pesticide at the early stage of potato late blight, spraying the leaves in a volume sufficient to avoid dripping. Apply the pesticide twice, checking the disease baseline before the first application. Apply the pesticide once every seven days, and check the disease index seven days after each application to calculate the control effect.

[0221] Survey method: According to the severity of potato late blight, samples were taken at five diagonal points in each plot, two plants were surveyed at each point, all leaves were checked, and the total number of plants, number of diseased plants and disease level were recorded.

[0222] The grading standards are as follows:

[0223] Level 0: no lesions;

[0224] Level 1: The lesion area accounts for less than 5% of the total leaf area;

[0225] Level 3: The lesion area accounts for 6% to 10% of the total leaf area;

[0226] Level 5: The lesion area accounts for 11% to 20% of the total leaf area;

[0227] Level 7: The lesion area accounts for 21% to 50% of the total leaf area;

[0228] Level 9: The area of lesions accounts for more than 50% of the total leaf area.

[0229] Calculation of drug efficacy:

[0230]

[0231]

[0232] Where:

[0233] CK0—pre-drug condition index of blank control area; CK1—post-drug condition index of blank control area;

[0234] PT0—the disease index before drug treatment in the drug-treated area; PT1—the disease index after drug treatment in the drug-treated area.

[0235] The test results are shown in Table 3.

[0236] Table 3: Effects of different pesticides on potato late blight

[0237]

[0238]

[0239]

[0240] As shown in Table 3, the combined compositions of polyoxin and cyfluthrin, and polyoxin and epoxiconazole, were significantly more effective than the control agents in preventing and controlling potato late blight, and their quick-acting properties were improved. Furthermore, no phytotoxicity was observed in the combined compositions during indoor and outdoor bioassays. Therefore, the fungicidal compositions and formulations of the present invention are safe and effective, can reduce environmental pollution caused by pesticides, and lower the cost of plant disease control, thus possessing excellent application value.

[0241] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0242] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0243] In addition, the various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. A composition, characterized in that: The composition comprises active ingredient A and active ingredient B, wherein active ingredient A is polyoxin, and active ingredient B is a triazole sulfonamide fungicide; wherein the triazole sulfonamide fungicide is selected from any one or two of cyfluthrin and epoxiconazole; The mass ratio of active ingredient A to active ingredient B is (1-30):(30-1); The structure of cyfluthrin is ， The structure of epoxiconazole is 。 2. The composition according to claim 1, wherein: The mass ratio of active ingredient A to active ingredient B is (1~20):(20~1).

3. The composition according to claim 2, characterized in that: The mass ratio of active ingredient A to active ingredient B is (1~10):(10~1).

4. The composition according to claim 3, characterized in that: The mass ratio of active ingredient A to active ingredient B is (1~5):(10~1).

5. The composition according to claim 4, characterized in that: The mass ratio of active ingredient A to active ingredient B is (1~5):(5~1).

6. The composition according to any one of claims 1 to 5, characterized in that: Based on the total content of the composition being 100% by mass, the sum of the contents of the active ingredient A and the active ingredient B in the composition is 1 to 80% by mass.

7. The composition according to claim 6, characterized in that: The total content of active ingredient A and active ingredient B in the composition is 1 to 60% by mass.

8. The composition according to claim 7, characterized in that: The total content of active ingredient A and active ingredient B in the composition is 5 to 60% by mass.

9. The composition according to any one of claims 1 to 5, characterized in that: The composition further comprises adjuvants commonly used in pesticides.

10. A preparation, characterized in that: The preparation is prepared from the composition according to any one of claims 1 to 9.

11. The preparation according to claim 10, characterized in that: The formulation is in the form of any one of wettable powder, water dispersible granules or suspension.

12. Use of the composition according to any one of claims 1 to 9 or the preparation according to claim 10 or 11 in preventing and controlling plant diseases; the plants are selected from one or more of cereals, vegetables, fruits and flowers; and the diseases are selected from one or more of downy mildew, late blight and downy mildew.

13. The use according to claim 12, characterized in that: The plant diseases are selected from potato late blight, tomato late blight, pepper blight, watermelon blight, ginseng blight, cucumber downy mildew, grape downy mildew, cabbage downy mildew and / or litchi downy mildew.

14. The use according to claim 13, characterized in that: The plant diseases are selected from cucumber downy mildew, potato late blight and / or tomato late blight.

15. A method for preventing and controlling plant diseases, characterized in that: The method comprises applying an effective amount of the composition of any one of claims 1 to 9 or the formulation of claim 10 or 11 to the plant and / or plant part.

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