Ligands for Corrosion Stability Chromatography
By introducing multiple Staphylococcus A protein domains into the chromatographic analysis ligand and performing amino acid mutations, the problem of insufficient stability of the existing ligand in alkaline cleaning was solved, and the effect of maintaining efficient binding ability in high-concentration NaOH solution was achieved, which is suitable for low-cost and efficient purification of immunoglobulins.
Patent Information
- Application Number
- CN202210554191.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Priority Date
- 2008-12-24
- Filing Date
- 2009-12-23
- Publication Date
- 2025-09-16
- Estimated Expiration
- 2029-12-23
AI Technical Summary
Existing chromatographic analysis ligands based on Staphylococcus A protein are not stable enough when cleaned under alkaline conditions, resulting in a decrease in binding ability, affecting purification efficiency and cost.
A ligand for alkaline-stable chromatographic analysis has been developed, which contains multiple Staphylococcus A protein domains or their functional fragments. The alkaline resistance of the ligand is improved by attaching it to the resin at multiple points and performing amino acid mutations, for example, introducing a specific amino acid mutation at position 24 to enhance stability.
The stability of the ligand is significantly improved in an alkaline environment, and the efficient binding ability is maintained. It is suitable for low-cost and efficient purification of immunoglobulins and can be used for a long time in high-concentration NaOH solution without losing function.