Displacement device for gas in a pipette

Abstract

The application provides a displacement device for gas in a pipette, comprising a receiving mechanism and an injection mechanism. The receiving mechanism comprises a receiving vessel containing liquid, and a first connecting pipe, wherein a first end of the first connecting pipe is sleeved outside a liquid outlet of the pipette, and a second end of the first connecting pipe extends into the liquid in the receiving vessel. The injection mechanism comprises a jet head, a second connecting pipe connected with a free end of the jet head, and a gas pump connected with a free end of the second connecting pipe. The jet head is configured to enter the pipette through the liquid outlet, and displace aerosol in the pipette under the action of the gas pump. The application has the advantages of simple structure, convenient operation, and convenient installation and disassembly.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of gene amplification, and in particular to a displacement device for gas in a pipette. BACKGROUND

[0002] Pipettes are commonly used instruments in laboratories, which are mainly used for quantitative transfer of liquid. When pipettes transfer liquid in the laboratory, aerosols are generated, and the aerosols will form diffusion through different media, thereby causing more places to be seriously contaminated. For example, the generated aerosols will enter the pipette along with the liquid and be transferred to a new experimental test tube, thereby causing multi-aspect contamination, so that the final detection data is inaccurate.

[0003] However, there is still no device in the prior art to solve the above problems. SUMMARY

[0004] The purpose of the present application is to provide a displacement device for gas in a pipette, which can more quickly and more fully displace the aerosols in the pipette into a receiving vessel to eliminate the aerosols, thereby avoiding contamination of the reagent tube by the aerosols, and further improving the accuracy of the detection data.

[0005] According to the present application, a displacement device for gas in a pipette is provided, comprising a receiving mechanism and an injection mechanism, the receiving mechanism comprising a receiving vessel containing liquid, and a first connecting pipe, wherein a first end of the first connecting pipe is sleeved outside a liquid outlet of the pipette, and a second end extends into the liquid in the receiving vessel, the injection mechanism comprising a injection head capable of penetrating at least partially into the first connecting pipe, a second connecting pipe connected to a free end of the injection head, and a gas pump connected to a free end of the second connecting pipe, wherein the injection head is configured to enter the pipette through the liquid outlet, and displace the aerosols in the pipette under the action of the gas pump.

[0006] In one embodiment, the injection head comprises a first injection part configured as a cap, and a second injection part configured as a hollow rod.

[0007] In one embodiment, the diameter of the second injection part is smaller than the diameter of the liquid outlet of the pipette, thereby allowing the aerosols to flow to the first connecting pipe.

[0008] In one embodiment, a sharp portion is formed by radially inwardly inclined extension at the end of the second injection part.

[0009] In one embodiment, the diameter of the first connecting pipe is larger than the diameter of the second connecting pipe.

[0010] In one embodiment, the first connecting pipe and the second connecting pipe are both made of rubber.

[0011] In one embodiment, the displacement device for gas in a pipette comprises a first gas pipe and a second gas pipe arranged at both ends of the gas pump, wherein the first gas pipe is in communication with a gas source, and the second gas pipe extends into the second connecting pipe to form a sealed connection.

[0012] In one embodiment, the end of the second gas pipe is formed into a port extending obliquely in the transverse direction.

[0013] In one embodiment, the second gas pipe is configured as a rigid pipe, and the diameter of the second gas pipe is larger than the diameter of the second connecting pipe.

[0014] In one embodiment, the receiving vessel comprises at least one of a cylindrical shape or a square cylindrical shape. BRIEF DESCRIPTION OF DRAWINGS

[0015] The present application will be described in detail below with reference to the accompanying drawings, in which:

[0016] Figure 1 The structure of the displacement device for gas in a pipette according to the present application is schematically shown.

[0017] In the drawings, the same components are designated by the same reference numerals. The drawings are not drawn to scale. DETAILED DESCRIPTION

[0018] The present application will be described in detail below with reference to the accompanying drawings, in which:

[0019] Figure 1 The structure of the displacement device for gas in a pipette 100 according to the present application is schematically shown. As Figure 1 described above, the displacement device for gas in a pipette 100 according to the present application comprises a receiving mechanism 10. The receiving mechanism 10 comprises a receiving vessel 11 and a first connecting pipe 12. The receiving vessel 11 is configured in the shape of a cylinder or a square cylinder, and the receiving vessel 11 contains a liquid for eliminating subsequent aerosols discharged from the pipette 40. The first end of the first connecting pipe 12 is sleeved outside the liquid outlet 41 of the pipette 40, so that subsequent aerosols displaced from the pipette 40 can all flow to the first connecting pipe 12. The second end of the first connecting pipe 12 is connected to the receiving vessel 11. Specifically, the second end of the first connecting pipe 12 can extend into the interior of the receiving vessel 11 and be completely immersed in the liquid in the receiving vessel 11, so that the aerosols displaced from the pipette 40 can all flow into the liquid in the receiving vessel 11 through the first connecting pipe 12.

[0020] According to one embodiment of the present application, the aerosol is a gaseous dispersion system formed by dispersing and suspending solid or liquid small particles in a gaseous medium. The aerosol is composed of a plurality of small particles, generally sized from 0.001 μm to 100 μm, and the dispersion medium is generally a gas.

[0021] According to the present application, as shown in Figure 1 The injection mechanism 30 comprises an injection head 31, a second connecting pipe 32 and a gas pump 33. The free end of the injection head 31 is connected with the second connecting pipe 32. Specifically, the fixed end of the second connecting pipe 32 is sleeved on the injection head 31 to form a sealed connection. The free end of the second connecting pipe 32 is communicated with the gas pump 33, so that the gas pump 33 can deliver external gas into the second connecting pipe 32.

[0022] According to one embodiment of the present application, the injection head 31 can at least partially extend into the interior of the first connecting pipe 12 and enter into the pipette 40 through the liquid outlet 41. Thus, the injection head 31 can displace the aerosol in the pipette 40 under the action of the gas pump 33, and the displaced aerosol can flow into the receiving vessel 11 through the first connecting pipe 12. The details are described hereinafter.

[0023] According to one embodiment of the present application, as shown in Figure 1 The injection head 31 comprises a first injection portion 311 and a second injection portion 312. The first injection portion 311 is configured in a cap-shaped structure and is installed in the interior of the fixed end of the second connecting pipe 32 for receiving gas from the second connecting pipe 32. The second injection portion 312 is configured in a hollow rod-shaped structure and can sequentially pass through the first connecting pipe 12 and the liquid outlet 41 to enter into the pipette 40. In this way, the injection head 31 can more easily and completely displace the aerosol in the pipette 40 under the action of the gas pump 33.

[0024] It is easily understood that the injection head 31 can be adaptively replaced according to the range of the pipette 40. That is, a smaller-diameter injection head 31 is adapted to a small-range pipette 40, and a larger-diameter injection head 31 is adapted to a large-range pipette 40.

[0025] According to one embodiment of the present application, the diameter of the second injection portion 312 is smaller than the diameter of the liquid outlet 41 of the pipette 40. In this way, when the second injection portion 312 enters into the pipette 40 through the liquid outlet 41, the liquid outlet 41 still has a gap for the aerosol in the pipette 40 to flow toward the first connecting pipe 12. In this way, the injection head 31 can more easily and completely displace the aerosol in the pipette 40 under the action of the gas pump 33.

[0026] According to one embodiment of the present application, the end of the second injection portion 312 is inclined to extend radially inwardly to form a sharp portion. In this way, the second injection portion 312 can be more easily punctured through the first connecting tube 12 to enter into the pipette 40 through the liquid outlet 41. It is easily understood that, since the sharp portion is thin, after penetrating into the interior of the first connecting tube 12, the first connecting tube 12 has a certain sealing with the outer wall of the second injection portion 312, thereby effectively reducing the possibility of aerosol overflow during the displacement work.

[0027] In one embodiment of the present application, the diameter of the first connecting tube 12 is larger than the diameter of the second connecting tube 32. In this way, on the one hand, the gas delivered by the gas pump 33 can more easily enter into the pipette 40 through the second connecting tube 32 and the injection head 31. On the other hand, the aerosol displaced from the pipette 40 can more easily enter into the liquid in the receiving vessel 11 through the first connecting tube 12.

[0028] According to one embodiment of the present application, the first connecting tube 12 and the second connecting tube 32 are both made of rubber material. In this way, on the one hand, the first end of the first connecting tube 12 and the liquid outlet 41, the free end of the second connecting tube 32 and the gas pump 33, and the fixed end of the second connecting tube 32 and the first injection portion 311 can all form a sealed connection. On the other hand, since the connecting tube of rubber material is a common and low-cost material on the market, the production cost can be effectively reduced, thereby maximizing the benefits. In addition, since the rubber material has good air tightness, the possibility of aerosol and gas leakage can be effectively reduced during the displacement work.

[0029] According to one embodiment of the present application, as shown in Figure 1 The gas displacement device 100 for the pipette further includes a first gas tube 331 and a second gas tube 332. The first gas tube 331 is arranged at one end of the gas pump 33 and communicates with an external gas source for receiving gas. The second gas tube 332 is arranged at the other end of the gas pump 33 and can extend into the second connecting tube 32 to form a sealed connection with the second connecting tube 32. In this way, the gas received by the gas pump 33 can be more fully delivered into the second connecting tube 32 through the second gas tube 332, thereby providing sufficient gas supply for subsequent displacement work.

[0030] In one embodiment of the present application, the end of the second gas tube 332 is formed as a port inclined to extend in the transverse direction. In this way, the gas in the gas pump 33 can be more easily released, so that the aerosol in the pipette 40 can be more fully displaced into the liquid in the receiving vessel 11.

[0031] In one embodiment of the invention, the second air tube 332 is constructed as a rigid pipe, and the diameter of the second air tube 332 is larger than the diameter of the second connecting tube 32. In this way, on the one hand, the second connecting tube 32, which is a rubber tube, can be more easily fitted onto the second air tube 332. On the other hand, after the second connecting tube 32 is fitted onto the second air tube 332, the air pump 33 and the second connecting tube 32 can have better sealing.

[0032] The working process of this invention is described below, including the following steps:

[0033] First, connect one end of the first connecting tube 12 to the outlet 41 on the pipette 40 in a sealed manner, and then insert the other end of the first connecting tube 12 into the liquid surface of the receiving dish 11.

[0034] Then, one end of the second connecting tube 32 is sealed to the second air tube 332 of the air pump 33, and the other end of the second connecting tube 32 is sealed to the injection head 31.

[0035] Then, the second injection portion 312 of the injection head 31 passes through the first connecting tube 12 and the outlet 41 in sequence and enters the pipette 40.

[0036] Finally, the air pump is powered on, and the gas from the first air tube 331 passes sequentially through the second air tube 332, the second connecting tube 32, and the injection head 31, thus entering the pipette 40 and blowing out the aerosol inside the pipette 40. At this time, the aerosol flows sequentially through the liquid outlet 41 and the first connecting tube 12 into the receiving dish 11, and the aerosol dissolves in the liquid.

[0037] In one embodiment of the present invention, compared with existing aerosol removal devices, the present invention has the advantages of simple and quick operation steps, and no need for repeated disassembly and installation. Furthermore, the present invention has higher precision and measurement accuracy compared with existing devices.

[0038] In the first embodiment of the present invention, the following contents are included.

[0039] Experimental objective: To investigate the effect of aerosols on the Ct value of enterocytozoon hepatocellular carcinoma, a pathogen found in Litopenaeus vannamei.

[0040] Experimental materials: amplification reaction solution, 10 different negative samples of Litopenaeus vannamei-Enterocystis hepatis (No. 1, No. 2, No. 3, No. 4, No. 5, No. 6, No. 7, No. 8, No. 9, No. 10), 1 positive sample of Litopenaeus vannamei-Enterocystis hepatis (control group), and 22 test tubes.

[0041] Experimental instruments: quantitative fluorescence amplification instrument, pipettes contaminated with aerosols.

[0042] Experiment content:

[0043] 1. Detection before the removal of aerosol by pipette:

[0044] 1.1 The amplification reaction solution was respectively loaded into different 11 test tubes;

[0045] 1.2 10 negative samples of Penaeus vannamei - Hepatopanosus and 1 positive sample of Penaeus vannamei - Hepatopanosus were respectively extracted by pipette (without removing aerosol) and added to the above test tubes;

[0046] 1.3 The test tubes were mixed and centrifuged;

[0047] 1.4 The machine was loaded, and the amplification detection was performed by the fluorescence quantitative amplifier. The specific detection process was as follows: 50℃ 30s; 95℃ 120s; (95℃ 5s, 58℃ 10s;) 40 cycles; 4℃ storage.

[0048] 2. Detection after the removal of aerosol by pipette:

[0049] 2.1 The amplification reaction solution was respectively loaded into different 11 test tubes;

[0050] 2.2 The aerosol in the pipette was removed by the displacement device 100 for gas in the pipette;

[0051] 2.3 10 negative samples of Penaeus vannamei - Hepatopanosus and 1 positive sample of Penaeus vannamei - Hepatopanosus were respectively extracted by pipette (without removing aerosol) and added to the above test tubes;

[0052] 2.4 The test tubes were mixed and centrifuged;

[0053] 2.4 The machine was loaded, and the amplification detection was performed by the fluorescence quantitative amplifier. The specific detection process was as follows: 50℃ 30s; 95℃ 120s; (95℃ 5s, 58℃ 10s;) 40 cycles; 4℃ storage.

[0054] Experimental results:

[0055]

[0056]

[0057] According to the results, when the pipette without removing aerosol was used as a liquid taking tool, 6 false positive samples appeared in 10 negative samples. When the pipette without removing aerosol was used as a liquid taking tool, no false positive sample appeared in 10 negative samples. Therefore, the displacement device 100 for gas in the pipette has good effect of removing aerosol in the pipette. In this way, the influence of aerosol on the Ct value of the detection sample can be avoided, thereby improving the accuracy of the detection data.

[0058] The present application can displace the aerosol in the pipette 40 into the receiving vessel 11 more quickly and more fully to eliminate the aerosol, thereby avoiding contamination of the reagent tube by the aerosol and further improving the accuracy of the detection data. In addition, the present application has a simple structure, is convenient to operate, and is convenient to install and disassemble.

[0059] The above is only the preferred embodiment of the present application, but the protection scope of the present application is not limited thereto. Those skilled in the art can easily make changes or variations within the scope of the disclosure of the present application, and such changes or variations should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A device for replacing gas in a pipette, characterized in that, include: Receiving mechanism (10) and injection mechanism (30), The receiving mechanism (10) includes a receiving dish (11) containing liquid and a first connecting tube (12), wherein a first end of the first connecting tube (12) is sleeved outside the outlet (41) of the pipette (40), and a second end extends into the liquid in the receiving dish (11). The injection mechanism (30) includes an injection head (31) capable of at least partially penetrating the first connecting tube (12), a second connecting tube (32) connected to the free end of the injection head (31), and an air pump (33) connected to the free end of the second connecting tube (32). The injection head (31) is configured to enter the pipette (40) through the outlet (41) and, under the action of the air pump (33), displace the aerosol inside the pipette (40). The injection head (31) includes a first injection portion (311) configured as a cap and a second injection portion (312) configured as a hollow rod. The diameter of the second injection portion (312) is smaller than the diameter of the outlet (41) of the pipette (40), thereby allowing the aerosol to flow to the first connecting tube (12). A spike is formed at the end of the second injection portion (312) extending radially inward at an inward angle. The device for replacing gas in a pipette includes a first air pipe (331) and a second air pipe (332) disposed at both ends of the air pump (33), wherein the first air pipe (331) is connected to a gas source, and the second air pipe (332) extends into the second connecting pipe (32) to form a sealed connection.

2. The device for replacing gas in a pipette according to claim 1, characterized in that, The diameter of the first connecting pipe (12) is larger than the diameter of the second connecting pipe (32).

3. The device for replacing gas in a pipette according to claim 2, characterized in that, Both the first connecting tube (12) and the second connecting tube (32) are made of rubber.

4. The device for replacing gas in a pipette according to claim 1, characterized in that, At the end of the second trachea (332), a port is formed that extends obliquely in the lateral direction.

5. The device for replacing gas in a pipette according to claim 4, characterized in that, The second trachea (332) is constructed as a rigid pipe, and the diameter of the second trachea (332) is larger than the diameter of the second connecting pipe (32).

6. The device for replacing gas in a pipette according to any one of claims 1 to 3, characterized in that, The receiver dish (11) includes at least one of a cylinder or a square tube.

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