Brucellosis Rose Bengal Analyzer

Abstract

The present invention relates to a brucellosis rose bengal analyzer, which includes a mounting base, a push plate mechanism, a liquid transfer mechanism, an oscillation mechanism and an interpretation mechanism. The mounting base is provided with a sample area, a reaction area, a recovery area and a pre-storage area. The push plate mechanism is arranged in the pre-storage area and includes a push plate, which can push the reaction plate in the pre-storage area to the reaction area and push the reaction plate in the reaction area to the recovery area. The liquid transfer mechanism is arranged above the mounting base, the oscillation mechanism is arranged in the reaction area and below the reaction plate, and the interpretation mechanism is arranged above the mounting base. The brucellosis rose bengal analyzer has a high degree of automation in the detection process, saves manual time, reduces labor costs, has high detection efficiency, and reduces personnel infection at the same time.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and particularly to a brucellosis rose bengal analyzer. Background Art

[0002] Brucellosis is an acute and chronic infectious disease that affects both humans and animals and is caused by Brucella. It is contracted by coming into contact with infected livestock or consuming contaminated milk or dairy products. The incubation period is 1-3 weeks. The clinical features are a slow onset, persistent fever, excessive sweating, weakness, general body aches, and joint pain. The symptoms of the acute phase usually subside within 3-6 months.

[0003] The rose bengal plate agglutination test (RBPT) is suitable for the general survey of brucellosis in groups. It is the designated test for the detection of Brucella in cattle, sheep, and pigs in international trade and is also used for the preliminary screening of human brucellosis surveillance in China. Since the nutritional conditions for Brucella culture are demanding and time-consuming, the diagnosis of brucellosis mainly relies on serological diagnosis. Its principle is mainly based on the specific binding of corresponding antigens and antibodies in vitro, resulting in a visible reaction, and using known antigens (or antibodies) to detect unknown antibodies (or antigens) in body fluids.

[0004] In the traditional rose bengal plate agglutination test, animal serum and rose bengal reagent need to be manually aspirated onto the reaction plate, manually mixed, and waited for 4 minutes. Then, the agglutination status is observed manually with the naked eye to determine negative or positive, which is inefficient and prone to causing personnel infection. Summary of the Invention

[0005] Based on this, in view of the problems of manual operation, easy infection, and low efficiency in traditional brucellosis detection, the present invention provides a brucellosis rose bengal analyzer.

[0006] A brucellosis rose bengal analyzer includes

[0007] a mounting base, which is provided with a sample area for placing samples, a reaction area for the reaction plate to react, a recovery area for recovering the reaction plate, and a pre-storage area for pre-storing the reaction plate. The pre-storage area is located at the front end of the reaction area, and the recovery area is located at the rear end of the reaction area;

[0008] a push plate mechanism, which is arranged in the pre-storage area. The push plate mechanism includes a movable push plate that can push the reaction plate preset in the pre-storage area to the reaction area and push the reaction plate in the reaction area to the recovery area;

[0009] a liquid transfer mechanism, which is arranged above the mounting base and is used to aspirate the sample from the sample area to the reaction plate in the reaction area and add reagents to the reaction plate in the reaction area;

[0010] An oscillation mechanism, which is arranged in the reaction area and below the reaction plate, is used to generate vibrations to mix the samples and reagents on the reaction plate;

[0011] An interpretation mechanism, which is arranged above the mounting base, is used to obtain an image of the reaction plate in the reaction area.

[0012] In one embodiment, the push plate mechanism further includes a support plate, a driving component and a stacking rack. The stacking rack is fixedly connected to the support plate. There is an outlet opening between the bottom end of the stacking rack and the support plate. The driving component can drive the push plate to push the lowermost reaction plate in the stacking rack through the outlet opening to the reaction area.

[0013] In one embodiment, the stacking rack includes a first enclosing plate, a second enclosing plate and a third enclosing plate connected in sequence. The first enclosing plate and the third enclosing plate are arranged opposite to each other and are respectively fixedly connected to the support plate. There is the outlet opening between the bottom end of the second enclosing plate and the support plate. The first enclosing plate, the second enclosing plate and the third enclosing plate enclose to form a limiting cavity for presetting the reaction plate.

[0014] In one embodiment, the driving component includes a push plate motor, a transmission belt and a connecting block. One end of the connecting block is connected to the push plate, and the other end of the connecting block is connected to the transmission belt. The transmission belt is in transmission connection with the push plate motor.

[0015] In one embodiment, the push plate mechanism includes a plurality of the stacking racks and a plurality of the push plates. The push plate mechanism further includes a feeding bottom plate and feeding blocks respectively connected to the feeding bottom plate. The plurality of stacking racks are arranged at intervals along the length direction of the feeding bottom plate. The push plates are arranged in one-to-one correspondence with the stacking racks. The support plate is provided with guide grooves corresponding to the stroke areas of the push plates. The feeding blocks are connected to the corresponding push plates through the guide grooves. The feeding bottom plate is connected to the connecting block.

[0016] In one embodiment, the push plate mechanism further includes a first limit switch and a second limit switch fixedly arranged on the bottom surface of the support plate. The first limit switch and the second limit switch are arranged at intervals. The feeding bottom plate is located between the first limit switch and the second limit switch;

[0017] And / or, the push plate mechanism further includes a guide rail fixedly arranged on the bottom surface of the support plate and a guide block fixedly arranged on the feeding bottom plate. The guide rail extends along the pushing direction of the push plate. The guide block is in sliding fit with the guide rail.

[0018] In one embodiment, the brucellosis rose bengal analyzer further includes a pressing plate mechanism disposed in the reaction area. The pressing plate mechanism includes a backlight plate and at least two pressing bars arranged at intervals. The pressing bars extend from the front end to the rear end of the reaction area and are fixed to the backlight plate. A concave position is provided on the side of the pressing bar. The concave positions of two adjacent pressing bars are arranged opposite to each other and enclose a limiting groove for accommodating the reaction plate with the backlight plate. The limiting groove is arranged corresponding to the push plate.

[0019] In one embodiment, the oscillating mechanism includes an oscillating motor, an eccentric wheel and an oscillating plate. The output shaft of the oscillating motor is connected to the eccentric wheel, and the eccentric wheel is connected to the oscillating plate.

[0020] In one embodiment, the brucellosis rose bengal analyzer further includes a pipe fitting fixing mechanism disposed in the sample area. The pipe fitting fixing mechanism includes a fixing frame and a sleeve. The fixing frame is provided with an installation groove, and the sleeve is detachably installed in the installation groove. The sleeve is provided with a pipe groove and a clamping groove arranged on one side of the upper end of the pipe groove.

[0021] In one embodiment, the mounting base is further provided with a reagent area for placing reagents and a pipette tip area for placing pipette tips. The liquid transfer mechanism includes a three-axis motion assembly and a pipettor located on the three-axis motion assembly. The three-axis motion assembly is used to drive the pipettor to move in the space above the mounting base.

[0022] For the above-mentioned brucellosis rose bengal analyzer, the sample is placed in the sample area. The reaction plate in the preset area is pushed to the reaction area and placed properly by the push plate of the push plate mechanism. The sample in the sample area is sucked to the reaction plate in the reaction area and reagents are added to the reaction plate in the reaction area by the liquid transfer mechanism. Then, the reaction plate with the sample and reagents added is vibrated by the oscillating mechanism to make them mix evenly. After the reaction plate stands for a period of time, the two react fully. At this time, the detection result on the reaction plate appears. For example, the positive serum shows an agglutination state, while the negative serum remains clear. The judgment mechanism moves above the reaction plate to take a photo of the detection result on the reaction plate to obtain image information. The image information can be judged by the detection software. The push plate of the push plate mechanism pushes again to push the reaction plate in the preset area to the reaction area. During the process of the new reaction plate moving to the reaction area, the used reaction plate will be pushed to the recovery area synchronously. The whole detection process has a high degree of automation, saves manual time, reduces labor costs, has high detection efficiency, and reduces the risk of personnel infection. Description of the Drawings

[0023] By reading the following detailed description with reference to the drawings, the above and other objects, features and advantages of the exemplary embodiments of the present disclosure will become readily understood. In the drawings, several embodiments of the present disclosure are shown in an exemplary rather than restrictive manner, and the same or corresponding reference numerals represent the same or corresponding parts, wherein:

[0024] Figure 1 Schematic structural diagram of a brucellosis rose bengal analyzer according to an embodiment of the present application;

[0025] Figure 2 is Figure 1 Schematic diagram after removing the casing of the brucellosis rose bengal analyzer in

[0026] Figure 3 Exploded schematic diagram of the pipe fitting fixing mechanism of a brucellosis rose bengal analyzer according to an embodiment of the present application;

[0027] Figure 4 is Figure 3 Assembly schematic diagram of the pipe fitting fixing mechanism of

[0028] Figure 5 Stereoscopic schematic diagram of the push plate mechanism of a brucellosis rose bengal analyzer according to an embodiment of the present application;

[0029] Figure 6 、 7 is Figure 5 Side view schematic diagram of the push plate mechanism of

[0030] Figure 8 Stereoscopic schematic diagram of the push plate mechanism of a brucellosis rose bengal analyzer according to an embodiment of the present application from another perspective;

[0031] Figure 9 Bottom view schematic diagram of the push plate mechanism of a brucellosis rose bengal analyzer according to an embodiment of the present application;

[0032] Figure 10 Exploded schematic diagram of the pressing plate mechanism and the oscillating mechanism of a brucellosis rose bengal analyzer according to an embodiment of the present application;

[0033] Figure 11 Assembly schematic diagram of the pressing plate mechanism and the oscillating mechanism of a brucellosis rose bengal analyzer according to an embodiment of the present application;

[0034] Figure 12 is Figure 11 Front view schematic diagram of the pressing plate mechanism and the oscillating mechanism of

[0035] Figure 13 is Figure 11 Side view schematic diagram of the pressing plate mechanism and the oscillating mechanism of

[0036] Figure 14 Schematic structural diagram of the pressing plate mechanism of a brucellosis rose bengal analyzer according to an embodiment of the present application;

[0037] Figure 15 Schematic structural diagram of the oscillating mechanism of a brucellosis rose bengal analyzer according to an embodiment of the present application.

[0038] Description of the reference numerals in the drawings:

[0039] 10. Mounting base; 20. Pusher plate mechanism; 210. Pusher plate; 220. Support plate; 222. Guide groove; 230. Driving assembly; 231. Pusher plate motor; 232. Transmission belt; 2321. First belt; 2322. Second belt; 233. Connecting block; 240. Palletizing rack; 201. Plate outlet; 202. Limiting cavity; 242. First enclosing plate; 244. Second enclosing plate; 246. Third enclosing plate; 248. Fourth enclosing plate; 249. Window; 250. Feeding bottom plate; 260. Feeding block; 270. First limit switch; 280. Second limit switch; 291. Guide rail; 292. Guide block; 30. Pipetting mechanism; 310. Three-axis motion assembly; 320. Pipettor; 40. Oscillation mechanism; 410. Oscillation motor; 420. Eccentric wheel; 430. Oscillation plate; 440. Elastic member; 450. Follow-up block; 460. Bearing plate; 470. Vertical plate; 480. Support bottom plate; 60. Reaction plate; 70. Pressing plate mechanism; 710. Backlight plate; 720. Pressing strip; 7202. First pressing strip; 7201. Second pressing strip; 722. Concave position; 724. Limiting groove; 80. Pipe fixing mechanism; 810. Fixing frame; 812. Mounting groove; 820. Sleeve; 822. Pipe groove; 824. Card slot; 830. 1.5 ml conical bottom sample tube; 840. 2 ml round bottom sample tube; 850. Cap; 91. Tip rack; 92. Reagent rack; 93. Reagent bottle; 100. Machine housing; 110. Recycling bin. Detailed implementation manners

[0040] Next, the technical solutions in the embodiments of the present disclosure will be clearly and completely described in conjunction with the drawings in the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, rather than all the embodiments. Based on the embodiments in the present disclosure, all other embodiments obtained by those skilled in the art without creative efforts shall fall within the protection scope of the present disclosure.

[0041] Next, the detailed implementation manners of the present disclosure will be described in detail in conjunction with the drawings.

[0042] Refer to Figure 2 , a brucellosis rose bengal analyzer provided by an embodiment of the present invention includes a mounting base 10, a pusher plate mechanism 20, a pipetting mechanism 30, an oscillation mechanism 40 and an interpretation mechanism.

[0043] Further, the mounting base 10 is provided with a sample area for placing samples, a reaction area for the reaction plate 60 to react, a recovery area for recovering the reaction plate 60, and a storage area for pre-storing the reaction plate 60. The storage area is located at the front end of the reaction area, and the recovery area is located at the rear end of the reaction area. Herein, the front end and the rear end are referenced based on the sequence of the movement path of the reaction plate 60. The position where the movement path is earlier is the front end, and vice versa is the rear end.

[0044] Further, the push plate mechanism 20 is arranged in the storage area. The push plate mechanism 20 includes a movable push plate 210. The push plate 210 can push the reaction plate 60 preset in the storage area to the reaction area and push the reaction plate 60 in the reaction area to the recovery area. The storage area pre-stores unused reaction plates 60, which are moved to the reaction area by the push plate 210. At the same time, the used reaction plates 60 originally in the reaction area are also pushed to the recovery area by the unused reaction plates 60 entering from the front end.

[0045] Further, the liquid transfer mechanism 30 is arranged above the mounting base 10, and is used for sucking the sample in the sample area to the reaction plate 60 in the reaction area and adding reagents to the reaction plate 60 in the reaction area. Optionally, the reagents can be pre-stored in the reagent tubes on the liquid transfer mechanism 30. When the liquid transfer mechanism 30 moves above the reaction area, the reagents in the reagent tubes can be dropped onto the reaction plate 60 in the reaction area according to the actual demand; or, the mounting base 10 is further provided with a reagent area for placing reagents. The liquid transfer mechanism 30 can be first moved above the reagent area and then lowered to suck a certain amount of reagents from the reagent bottles 93 in the reagent area, and then moved above the reaction area to drop the reagents onto the reaction plate 60.

[0046] Further, the oscillation mechanism 40 is arranged in the reaction area and below the reaction plate 60, and is used for generating vibrations to mix the samples and reagents on the reaction plate 60. The oscillation mechanism 40 can generate fixed-frequency eccentric vibrations or variable-frequency eccentric vibrations, driving the reaction plate 60 in the reaction area to vibrate synchronously, so as to mix the samples and reagents on the reaction plate 60 fully and react, improving the accuracy of the detection results.

[0047] Further, the interpretation mechanism is arranged above the mounting base 10 and is used for acquiring images of the reaction plate 60 in the reaction area. The judgment mechanism takes pictures or videos of the reaction results of the samples and reagents on the reaction plate 60, and the acquired images of the reaction plate 60 can be transmitted to the detection software through wired or wireless means to automatically judge the detection results.

[0048] The above-mentioned brucellosis rose bengal analyzer places the sample in the sample area. The reaction plate 60 in the preset area is pushed to the reaction area and placed properly by the movement of the push plate 210 of the push plate mechanism 20. The sample in the sample area is aspirated to the reaction plate 60 in the reaction area and reagents are added to the reaction plate 60 in the reaction area through the liquid transfer mechanism 30. Then, the reaction plate 60 with the sample and reagents added is vibrated by the oscillation mechanism 40 to make it evenly mixed. After the reaction plate 60 stands still for a period of time, the two react fully. At this time, the detection result on the reaction plate 60 appears. For example, the positive serum shows an agglutination state, while the negative serum remains clear. The judgment mechanism moves above the reaction plate 60 to take a photo of the detection result on the reaction plate 60 to obtain image information. The image information can be judged through the detection software. The push plate 210 of the push plate mechanism 20 pushes again to push the reaction plate 60 in the preset area to the reaction area. During the process of the new reaction plate 60 moving to the reaction area, the used reaction plate 60 will be synchronously pushed to the recovery area. The whole detection process has a high degree of automation, saves manual time, reduces labor costs, has a high detection efficiency, and reduces the risk of personnel infection.

[0049] Please refer to Figure 1 、 2 In one embodiment, the brucellosis rose bengal analyzer further includes a housing 100 surrounding the outer periphery of the mounting base 10. An exhaust fan and a filter element are provided on the housing 100. When the exhaust fan is turned on, the air inside the housing 100 passes through the filter element before being discharged. The detection process is completed inside the housing 100 to prevent contamination.

[0050] Please refer to Figure 3 、 4 The brucellosis rose bengal analyzer further includes a pipe fitting fixing mechanism 80. The pipe fitting fixing mechanism 80 is arranged in the sample area and is used for installing sample tubes.

[0051] In one embodiment, the pipe fitting fixing mechanism 80 includes a fixing frame 810 and a sleeve 820. The fixing frame 810 is provided with an installation groove 812, and the sleeve 820 is detachably installed in the installation groove 812. The sleeve can be adapted to a disposable 1.5 ml pointed-bottom sample tube 830 or a 2 ml round-bottom sample tube 840. When the sleeve 820 is taken out of the installation groove 812, the fixing frame 810 can be suitable for installing a 5 ml negative pressure blood collection tube, and the usage scenario is flexible.

[0052] A sample identifier is provided in the sample area, and a ceiling lamp is also provided on the housing 100. The sample identifier is arranged at the bottom end of the fixing frame 810. When a sample tube is inserted into the fixing frame 810, the sample identifier can sense it and automatically identify the position and quantity of the samples placed. Optionally, the sample identifier is a photoresistor.

[0053] Further, the sleeve 820 is provided with a tube slot 822 and a card slot 824 disposed on one side of the upper end of the tube slot 822. When the sample tube is placed in the tube slot 822 of the sleeve 820 and the lid of the sample tube is opened, its lid 850 can be stuck in the card slot 824 of the sleeve 820 to prevent the lid 850 of the sample tube from falling off and blocking the opening, which may affect the automatic sampling of the liquid transfer mechanism 30.

[0054] Please refer to Figure 2 , optionally, in one embodiment, the mounting base 10 is further provided with a reagent area for placing reagents. The reagent area is provided with a reagent rack 92 for mounting reagent bottles 93. Further, the mounting base 10 is further provided with a tip area for placing tips. The tip area is provided with a tip rack 91, and the tip rack 91 is provided with a plurality of mounting holes for mounting disposable tips. Before each liquid transfer, the liquid transfer mechanism 30 can first move to the tip area to automatically assemble the tips and then aspirate the sample or reagent, and then automatically discard the used disposable tips after dropping the liquid.

[0055] In one embodiment, the liquid transfer mechanism 30 includes a three-axis motion assembly 310 and a pipettor 320 located on the three-axis motion assembly 310. The three-axis motion assembly 310 is used to drive the pipettor 320 to move in the space above the mounting base 10. The three-axis motion assembly 310 includes X, Y, and Z-axis motion tracks and can move in three directions of X, Y, and Z in the space above the mounting base 10. The pipettor 320 can be adapted to the disposable tips in the tip area.

[0056] Optionally, a plurality of pipettors 320 can be provided. Figure 1 Two pipettors 320 are shown in

[0057] Refer to Figure 2 、 5-9, in one embodiment, the push plate mechanism 20 further includes a support plate 220, a driving assembly 230, and a palletizing rack 240. The palletizing rack 240 is fixedly connected to the support plate 220. There is a plate outlet 201 between the bottom end of the palletizing rack 240 and the support plate 220. The driving assembly 230 can drive the push plate 210 to push the lowermost reaction plate 60 in the palletizing rack 240 through the plate outlet 201 to the reaction area. Multiple reaction plates 60 can be pre-stored in the palletizing rack 240. Since there is a plate outlet 201 between the bottom end of the palletizing rack 240 and the support plate 220, and the height of the plate outlet 201 is greater than or equal to the thickness of the reaction plate 60, the push plate 210 pushes the lowermost reaction plate 60 from the side opposite to the plate outlet 201, causing the reaction plate 60 to move to the reaction area accordingly. Then, the driving assembly 230 drives the push plate 210 to move in the reverse direction and withdraw from the palletizing rack 240. The reaction plates 60 in the palletizing rack 240 fall onto the support plate 220 due to gravity. The driving assembly 230 drives the push plate 210 to move towards the plate outlet 201 again, pushing a new reaction plate 60 to the reaction area. During the movement of the new reaction plate 60, it can push the previous reaction plate 60 towards the recovery area, causing the reaction plate 60 closest to the recovery area in the reaction area to fall into the recovery area for recovery.

[0058] Refer to Figure 5 , optionally, in one embodiment, the palletizing rack 240 includes a first enclosing plate 242, a second enclosing plate 244, and a third enclosing plate 246 that are connected in sequence. The first enclosing plate 242 and the third enclosing plate 246 are oppositely arranged and are respectively fixedly connected to the support plate 220. There is the plate outlet 201 between the bottom end of the second enclosing plate 244 and the support plate 220. The first enclosing plate 242, the second enclosing plate 244, and the third enclosing plate 246 enclose to form a limiting cavity 202 for presetting the reaction plate 60. The reaction plate 60 is square, and the limiting cavity 202 is a square cavity with a cross-section matching that of the reaction plate 60. The reaction plate 60 is limited by the first enclosing plate 242, the second enclosing plate 244, and the third enclosing plate 246, so that the stacked reaction plates 60 are limited in the limiting cavity 202 and will not collapse.

[0059] Further, in one embodiment, the pallet rack 240 further includes a fourth enclosing plate 248. The fourth enclosing plate 248 is disposed opposite to the second enclosing plate 244, and both sides of the fourth enclosing plate 248 are respectively connected to the first enclosing plate 242 and the third enclosing plate 246. A clearance opening is left between the bottom end of the fourth enclosing plate 248 and the support plate 220. That is, the first enclosing plate 242, the second enclosing plate 244, the third enclosing plate 246, and the fourth enclosing plate 248 form a quadrilateral hollow column structure to limit the periphery of the reaction plate 60. The push plate 210 extends into the limiting cavity 202 from the clearance opening to push the reaction plate 60. Optionally, a viewing window 249 is provided on the fourth enclosing plate 248, and the number of reaction plates 60 in the limiting cavity 202 can be quickly known through the viewing window 249, so as to add new reaction plates 60 in time.

[0060] Referring Figure 5 - 7 , in one embodiment, the driving assembly 230 includes a push plate motor 231, a transmission belt 232, and a connecting block 233. One end of the connecting block 233 is connected to the push plate 210, and the other end of the connecting block 233 is connected to the transmission belt 232. The transmission belt 232 is in transmission connection with the push plate motor 231. The push plate motor 231 drives the transmission belt 232 to rotate, synchronously drives the connecting block 233 connected to the transmission belt 232 to move, and further drives the push plate 210 to move in a direction close to or away from the plate outlet 201.

[0061] Specifically, the transmission belt 232 includes a first belt 2321 and a second belt 2322. Below the support plate 220, there are a first belt 2321 pulley, a second belt 2322 pulley, and a third pulley. Among them, the first belt 2321 pulley and the second belt 2322 pulley rotate coaxially. One end of the first belt 2321 is sleeved on the first belt 2321 pulley, and the other end is sleeved on the output shaft of the push plate motor 231. Both ends of the second belt 2322 are respectively sleeved on the second belt 2322 pulley and the third pulley. The connecting block 233 is connected to the second belt 2322. The push plate motor 231 drives the first belt 2321 to rotate, and then drives the first belt 2321 pulley and the second belt 2322 pulley to rotate simultaneously, drives the second belt 2322 to rotate clockwise or counterclockwise, so as to drive the connecting block 233 connected thereto to move forward or backward, thereby driving the push plate 210 to move in a direction close to or away from the plate outlet 201. With such a setting, it is convenient to reasonably arrange the push plate motor 231 and avoid affecting the operation of other structures. In other embodiments, the first belt 2321 may not be provided, and the second belt 2322 pulley or the third pulley is directly driven by the push plate motor 231 to drive the second belt 2322 to rotate.

[0062] Optionally, in one embodiment, the pusher mechanism 20 includes a plurality of the pallet racks 240 and a plurality of the push plates 210. Three pallet racks 240 and three push plates 210 are shown in the figure. Through the plurality of the pallet racks 240 and the plurality of the push plates 210, a plurality of reaction plates 60 can be pushed into the reaction zone simultaneously, improving the detection efficiency.

[0063] Referring to Figure 8 , 9 , further, the pusher mechanism 20 further includes a feeding bottom plate 250 and feeding blocks 260 respectively connected to the feeding bottom plate 250. The plurality of the pallet racks 240 are arranged at intervals along the length direction of the feeding bottom plate 250, and the push plates 210 are arranged in one-to-one correspondence with the pallet racks 240. The support plate 220 is provided with a guide groove 222 corresponding to the stroke area of the push plate 210, and the feeding block 260 is connected to the corresponding push plate 210 through the guide groove 222. The feeding bottom plate 250 is connected to the connecting block 233. The connecting block 233 moves along with the conveyor belt to drive the feeding bottom plate 250 to move, and further drives each feeding block 260 to move synchronously along the guide groove 222, thereby driving each push plate 210 to move synchronously, realizing that one push plate motor 231 drives a plurality of push plates 210 to move simultaneously, improving the working efficiency.

[0064] Optionally, in one embodiment, the pusher mechanism 20 further includes a first limit switch 270 and a second limit switch 280 fixedly arranged on the bottom surface of the support plate 220. The first limit switch 270 and the second limit switch 280 are arranged at intervals, and the feeding bottom plate 250 is located between the first limit switch 270 and the second limit switch 280. The moving stroke of the feeding bottom plate 250 is limited by the first limit switch 270 and the second limit switch 280 to prevent the feeding bottom plate 250 from moving too much.

[0065] Optionally, in one embodiment, the pusher mechanism 20 further includes a guide rail 291 fixedly arranged on the bottom surface of the support plate 220 and a guide block 292 fixedly arranged on the feeding bottom plate 250. The guide rail 291 extends along the pushing direction of the push plate 210, and the guide block 292 is slidably matched with the guide rail 291. Through the interactive cooperation between the guide block 292 and the guide rail 291, the smoothness of the forward or backward movement of the feeding bottom plate 250 is ensured.

[0066] Referring to Figure 10 - 14, in one embodiment, the brucellosis rose bengal analyzer further includes a pressing plate mechanism 70. The pressing plate mechanism 70 is disposed in the reaction area. The pressing plate mechanism 70 includes a backlight plate 710 and at least two pressing strips 720 arranged at intervals. The pressing strips 720 extend from the front end to the rear end of the reaction area and are fixed to the backlight plate 710. A concave position 722 is provided on the side of the pressing strip 720. The concave positions 722 of two adjacent pressing strips 720 are arranged oppositely and enclose a limiting groove 724 for accommodating the reaction plate 60 with the backlight plate 710. The limiting groove 724 is arranged corresponding to the pushing plate 210. The pushing plate 210 pushes the reaction plates 60 in the stacking rack 240 into the limiting groove 724 for sequential arrangement. Since the backlight plate 710 emits light to illuminate the reaction plates 60 thereon, the interpretation mechanism can obtain clear images of the reaction plates 60, improving the interpretation accuracy. The reaction plates 60 are limited to the backlight plate 710 by the pressing strips 720 provided with the concave positions 722, ensuring that the reaction plates 60 will not shake left and right and slide out of the backlight plate 710 during the vibration process.

[0067] Among them, the pressing strips 720 on both sides of the backlight plate 710 are the first pressing strips 7202 with only one concave position 722, and the pressing strips 720 in the middle of the backlight plate 710 are the second pressing strips 7201 with two concave positions 722. Two second pressing strips 7201 are shown in the figure, and three limiting grooves 724 are formed in the reaction area. According to actual requirements, the number of the second pressing strips 7201 can be flexibly increased or decreased. The two first pressing strips 7202 are respectively connected to the oscillation mechanism 40, and the backlight plate 710 is limited between the two first pressing strips 7202, so that the backlight plate 710 vibrates simultaneously with the oscillation mechanism 40. The two first pressing strips 7202 are located on the left and right sides of the backlight plate 710, and the front and rear sides of the backlight plate 710 are respectively butted against the pushing plate mechanism 20 and the recovery area. The front and rear ends of the limiting groove 724 are open, facilitating the entry and exit of the reaction plates 60. The reaction plates 60 are pushed into the limiting groove 724 by the pushing plate mechanism 20, and their two ends are respectively limited in the concave positions 722 of the two pressing strips 720.

[0068] Refer to Figure 10 - 13 , Figure 15, in one embodiment, the oscillation mechanism 40 includes an oscillation motor 410, an eccentric wheel 420 and an oscillation plate 430. The output shaft of the oscillation motor 410 is connected to the eccentric wheel 420, and the eccentric wheel 420 is connected to the oscillation plate 430. The oscillation mechanism 40 adopts the eccentric vibration principle. When the oscillation motor 410 rotates, it drives the eccentric wheel 420 to rotate, and the eccentric wheel 420 drives the oscillation plate 430 to vibrate, thereby driving the backlight plate 710 and the reaction plate 60 to vibrate, so that the reagent and the sample are mixed evenly. Three limiting slots 724 are shown in the figure. Each limiting slot 724 can limit 8 reaction plates 60. Each reaction plate 60 has 12 reaction cavities. Each vibration can complete the mixing of 288 samples without manual stirring, reducing manual waste and pollution. According to actual needs, the number of reaction plates 60 can be flexibly increased in the reaction area.

[0069] Specifically, in one embodiment, the first pressing strip 72027201 is connected to the oscillation plate 430, and the oscillation plate 430 limits the backlight plate 710 above it through the first pressing strips 7202 on both sides. Further, the oscillation mechanism 40 further includes an elastic member 440, a bearing plate 460 and a follower block 450. The oscillation plate 430 is connected to the bearing plate 460 through the elastic member 440 below. The oscillation motor 410 is installed on the bearing plate 460 and its output shaft is connected to the eccentric wheel 420. A follower block 450 is sleeved outside the eccentric wheel 420, and the follower block 450 is connected to the oscillation plate 430. When the oscillation motor 410 rotates, since one end of the eccentric wheel 420 is provided with a convex column and the other end is provided with a groove, and the central axes of the convex column and the groove do not coincide and there is an offset, the eccentric wheel 420 is sleeved on the output shaft of the oscillation motor 410 through the groove, and the follower block 450 is sleeved on the convex column. The output shaft of the oscillation motor 410 is fixed to the groove. When the oscillation motor 410 rotates, the eccentric wheel 420 rotates, and displacement is generated by the eccentricity, so that the oscillation plate 430 vibrates and drives the backlight plate 710 and the reaction plate 60 to vibrate, so that the sample and the reagent on the reaction plate 60 are mixed evenly. The elastic member 440 between the bearing plate 460 and the oscillation plate 430 plays a buffering role. Four elastic members 440 are provided, which are respectively arranged at the four corners of the oscillation plate 430. Optionally, the elastic member 440 is a rubber spring.

[0070] Optionally, the oscillation mechanism 40 further includes a vertical plate 470 and a support bottom plate 480. The bearing plate 460 is connected to the support bottom plate 480 through the vertical plate 470, and the oscillation motor 410 is located in the space formed by the support of the vertical plate 470. The support bottom plate 480 is fixed to the mounting seat 10.

[0071] In one embodiment, the interpretation mechanism includes a camera (not shown in the figure), the camera is connected to the three-axis motion assembly 310, and the three-axis motion assembly 310 is further configured to drive the camera to move in the space above the mounting base 10. The interpretation mechanism and the pipetting mechanism 30 can share the three-axis motion assembly 310 for movement. After the sample on the reaction plate 60 reacts with the reagent, the camera moves above the reaction plate 60 through the three-axis motion assembly 310 to take pictures, and the result is automatically judged by the AI algorithm. Compared with manual observation of the result, the judgment result is more accurate; the user can view the result through the client.

[0072] Referring to Figure 1 , in one embodiment, a recycling bin 110 is provided in the recycling area, and a disinfection lamp is provided in the recycling bin 110. The used reaction plate 60 is automatically pushed into the recycling bin 110, and the new reaction plate 60 is pushed to the reaction area. At the same time, the ultraviolet lamp in the recycling bin 110 is turned on for disinfection. There is no need for manual replacement of the reaction plate 60, reducing manual waste and pollution. Automatically disinfecting waste materials has low cost, timely disinfection, and avoids causing infections;

[0073] The working process of the brucellosis rose bengal analyzer in the above embodiment is as follows:

[0074] 1. The user clicks to turn on the machine at the instrument end. The user places the rose bengal reagent bottle 93 in the reagent area, places the serum sample in the sample area, evenly stacks the reaction plates 60 in the three stacking racks 240, and places the pipette tips in the pipette tip area. The user clicks start on the PC.

[0075] 2. The instrument cabin door closes, and the sample area automatically scans and identifies the position and quantity of the samples placed. The photoresistor fails, there is a ceiling lamp on top, and when a sample is placed, the photoresistor senses it. At the same time, the aerosol fan automatically turns on. This module is an exhaust fan, and the internal air is filtered before being discharged.

[0076] 3. Adding reagent: Two pipettors 320 automatically assemble pipette tips inside the instrument, move to the reagent area, suck the reagent and place it in the reaction cavity of the reaction plate 60. Then it moves above the trash chute and automatically discards the used disposable pipette tips. This process is repeated multiple times, and the number of repetitions is the same as the number of test samples.

[0077] 4. Adding sample: Two pipettors 320 automatically assemble pipette tips inside the instrument, move to the sample area, suck the sample serum and place it in the reaction cavity of the reaction plate 60. Then it moves above the trash chute and automatically discards the used disposable pipette tips. This process is repeated multiple times, and the number of repetitions is the same as the number of test samples.

[0078] 5. Oscillating and mixing: After adding the reagent and serum, the oscillating mechanism 40 below the reaction plate 60 starts to oscillate and mix to fully mix the reagent and the sample.

[0079] 6. Static reaction: After shaking and mixing evenly, the reaction plate is left standing at 60°C for 4 - 6 minutes for the two to fully react. At this time, the positive serum shows an agglutinated state, while the negative serum remains clear.

[0080] 7. Photographing and interpretation: The camera moves above the reaction plate and takes an automatic photograph, and the photo is transmitted to the software system for automatic result judgment.

[0081] 8. Disinfection: The used reaction plate is automatically pushed into the recycling box 110, and a new reaction plate is pushed to the reaction area. At the same time, the ultraviolet lamp in the recycling box 110 is turned on for disinfection.

[0082] 9. The client views the result.

[0083] In the above description of this specification, unless otherwise clearly specified and limited, terms such as "fixed", "installed", "connected" or "linked" should be understood in a broad sense. For example, in the case of the term "connected", it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, or it can be the internal communication of two components or the interaction relationship between two components. Therefore, unless otherwise clearly limited in this specification, those skilled in the art can understand the specific meaning of the above terms in the present invention according to specific circumstances.

[0084] According to the above description of this specification, those skilled in the art can also understand the terms used as follows. For example, terms indicating orientation or position relationship such as "upper", "lower", "front", "rear", "left", "right", "length", "width", "thickness", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", "center", "longitudinal", "transverse", "clockwise" or "counterclockwise" are based on the orientation or position relationship shown in the drawings of this specification. They are only for the purpose of facilitating the description of the solution of the present invention and simplifying the description, rather than explicitly or implicitly indicating that the device or component involved must have the specific orientation, be constructed and operate in the specific orientation. Therefore, the above terms of orientation or position relationship cannot be understood or interpreted as a limitation to the solution of the present invention.

[0085] In addition, the terms "first" or "second" etc. used in this specification to refer to numbers or ordinals are only for descriptive purposes and cannot be understood as explicitly or implicitly indicating relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" or "second" may explicitly or implicitly include at least one such feature. In the description of this specification, the meaning of "a plurality" is at least two, such as two, three or more, etc., unless otherwise clearly and specifically limited.

[0086] Although this specification has shown and described multiple embodiments of the present invention, it will be apparent to those skilled in the art that such embodiments are provided by way of example only. Those skilled in the art will think of many changes, alterations, and alternative ways without departing from the spirit and concept of the present invention. It should be understood that various alternatives to the embodiments of the present invention described herein may be employed in practicing the present invention. The appended claims are intended to define the scope of protection of the present invention and thus cover module components, equivalents, or alternatives within the scope of these claims.

Claims

1. A brucellosis rose bengal analyzer, characterized in that, Comprising A mounting base, which is provided with a sample area for placing samples, a reaction area for the reaction plate to react, a recovery area for recovering the reaction plate, and a pre-storage area for pre-storing the reaction plate. The pre-storage area is located at the front end of the reaction area, and the recovery area is located at the rear end of the reaction area; A push plate mechanism, which is arranged in the pre-storage area. The push plate mechanism includes a movable push plate, which can push the reaction plate preset in the pre-storage area to the reaction area and push the reaction plate in the reaction area to the recovery area. The push plate mechanism further includes a support plate, a driving component, and a stacking rack. The stacking rack is fixedly connected to the support plate, and there is an outlet between the bottom end of the stacking rack and the support plate. The driving component can drive the push plate to push the lowermost reaction plate in the stacking rack through the outlet to the reaction area; A liquid transfer mechanism, which is arranged above the mounting base and is used for sucking the sample in the sample area to the reaction plate in the reaction area and adding reagents to the reaction plate in the reaction area; An oscillation mechanism, which is arranged in the reaction area and below the reaction plate and is used for generating vibration to mix the sample and the reagent in the reaction plate; An interpretation mechanism, which is arranged above the mounting base and is used for acquiring the image of the reaction plate in the reaction area.

2. The Brucellosis Rose Bengal Analyzer according to claim 1, wherein The stacking rack includes a first enclosing plate, a second enclosing plate, and a third enclosing plate connected in sequence. The first enclosing plate and the third enclosing plate are arranged oppositely and are respectively fixedly connected to the support plate. There is the outlet between the bottom end of the second enclosing plate and the support plate. The first enclosing plate, the second enclosing plate, and the third enclosing plate enclose to form a limiting cavity for presetting the reaction plate.

3. The brucellosis rose bengal analyzer according to claim 1, wherein The driving component includes a push plate motor, a transmission belt, and a connecting block. One end of the connecting block is connected to the push plate, the other end of the connecting block is connected to the transmission belt, and the transmission belt is in transmission connection with the push plate motor.

4. The Brucellosis Rose Bengal Analyzer according to claim 3, wherein The push plate mechanism includes a plurality of the stacking racks and a plurality of the push plates. The push plate mechanism further includes a feeding bottom plate and feeding blocks respectively connected to the feeding bottom plate. The plurality of stacking racks are arranged at intervals along the length direction of the feeding bottom plate. The push plates are arranged in one-to-one correspondence with the stacking racks. The support plate is provided with a guide groove corresponding to the stroke area of the push plate. The feeding block is connected to the corresponding push plate through the guide groove, and the feeding bottom plate is connected to the connecting block.

5. The Brucellosis Rose Bengal Analyzer according to claim 4, wherein The push plate mechanism further includes a first limit switch and a second limit switch fixedly arranged on the bottom surface of the support plate. The first limit switch and the second limit switch are arranged at intervals, and the feeding bottom plate is located between the first limit switch and the second limit switch; And / or, the push plate mechanism further includes a guide rail fixedly arranged on the bottom surface of the support plate and a guide block fixedly arranged on the feeding bottom plate. The guide rail extends along the pushing direction of the push plate, and the guide block is in sliding fit with the guide rail.

6. The brucellosis rose bengal analyzer according to any one of claims 1-5, characterized in that, The brucellosis rose bengal analyzer further includes a pressing plate mechanism, which is arranged in the reaction area. The pressing plate mechanism includes a backlight plate and at least two pressing strips arranged at intervals. The pressing strips extend from the front end to the rear end of the reaction area and are fixed to the backlight plate. A concave position is provided on the side of the pressing strip, and the concave positions of two adjacent pressing strips are arranged oppositely and enclose a limiting groove for accommodating the reaction plate with the backlight plate. The limiting groove is arranged corresponding to the pushing plate.

7. The Brucellosis Rose Bengal Analyzer according to claim 6, wherein The oscillation mechanism includes an oscillation motor, an eccentric wheel and an oscillation plate. The output shaft of the oscillation motor is connected to the eccentric wheel, and the eccentric wheel is connected to the oscillation plate.

8. The brucellosis rose bengal analyzer according to any one of claims 1-5, characterized in that, The brucellosis rose bengal analyzer further includes a pipe fitting fixing mechanism, which is arranged in the sample area. The pipe fitting fixing mechanism includes a fixing frame and a sleeve. The fixing frame is provided with an installation groove, and the sleeve is detachably installed in the installation groove. The sleeve is provided with a pipe groove and a clamping groove arranged on one side of the upper end of the pipe groove.

9. The Brucellosis Rose Bengal Analyzer according to any one of claims 1-5, characterized in that, The mounting seat is further provided with a reagent area for placing reagents and a pipette tip area for placing pipette tips. The liquid transfer mechanism includes a three-axis motion assembly and a pipettor located on the three-axis motion assembly. The three-axis motion assembly is used to drive the pipettor to move in the space above the mounting seat.

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