Biological particle detection system and detection method

Abstract

A biological particle detection system includes a biological particle dilution device and a detection and extraction device; the biological particle dilution device includes a microfluidic channel, a carrier unit and a first optical detection component, the microfluidic channel is connected to a sample source providing target biological particles, the carrier unit includes a carrier for holding samples discharged through the microfluidic channel, and the first optical detection component provides a first detection light path to penetrate the microfluidic channel; when the first optical detection component senses that the target biological particles pass through the microfluidic channel, the carrier unit can provide a carrier, and the target biological particles are held on the carrier through the microfluidic channel; the carrier moves to the detection and extraction device; when the second optical detection component is used to locate the exact position of the target biological particles on the carrier, the extraction unit moves to the exact position, extracts the target biological particles, and moves them to a collection plate.

Description

Technical Field

[0001] The present invention relates to a biochemical detection system, and in particular to a biological particle detection system and a detection method. Background Art

[0002] It is known that traditional biochemical detection methods use artificial methods to detect biological samples using fluorescent staining and immunoprecipitation. However, traditional biochemical detection methods are limited by manual operation instruments and skills, so traditional biochemical detection requires a considerable amount of samples (such as blood, other body fluids, hair or nails, etc.), making it difficult to perform trace detection.

[0003] In addition, in addition to the difficulty of manual detection of trace amounts, traditional biochemical detection methods mostly use multiple independent detection equipment to perform large-scale to small-scale detection; generally speaking, after a large-scale screening is performed manually, the screened biological samples are transferred to another detection equipment for small-scale testing. The process often takes several minutes to several hours, and during the manual operation and transfer process, the biological samples often undergo qualitative changes or are contaminated due to long-term contact with the external environment. Furthermore, it is time-consuming to screen and separate trace samples from large volumes of biochemical specimens, and there is no good technology or method to accurately locate, identify and extract single biological particles from a large number of specimens without pre-treatment of the specimens.

[0004] Therefore, there is an urgent need for a novel biological particle detection system and detection method to overcome the above problems. Summary of the invention

[0005] In view of this, the purpose of the present invention is to provide a biological particle detection system and detection method, which can quickly and preliminarily screen a part of the specimen that may contain target biological particles from the specimen, further accurately examine the said part of the specimen, and accurately extract the target biological particles from the said part of the specimen. This method can avoid the waste of time caused by large-scale precise detection and the loss of specimens to be tested due to specimen pretreatment, while reducing the specimen volume to increase the detection efficiency. The present invention can efficiently and accurately locate, identify and extract single biological particles from a large range of specimens and avoid the conversion and change of the detection environment that causes qualitative changes or contamination of biological samples and the loss of specimens caused by specimen pretreatment.

[0006] In order to achieve the above-mentioned purpose, the present invention provides a biological particle detection system for detecting and collecting a target biological particle; the biological particle detection system includes a biological particle dilution device and a detection extraction device. The biological particle dilution device includes a microfluidic channel, a carrier unit and a first optical detection component. The microfluidic channel has a first end and a second end. The first end is connected to a sample source. The sample source provides a sample containing the target biological particle. The first optical detection component provides a first detection light path. The first detection light path penetrates the microfluidic channel and is used to sense the target biological particle. The carrier unit includes at least one carrier. When the first optical detection component senses that the target biological particle passes through the microfluidic channel, the carrier unit is controlled to provide a carrier so that the target biological particle passes through the microfluidic channel and the second end and is carried on the carrier. The detection and extraction device includes a second optical detection component and a extraction unit. The carrier is controlled to move to the detection and extraction device, and the second optical detection component corresponds to the carrier. The second optical detection component is used to scan, identify and locate the exact position of the target biological particle on the carrier. The extraction unit is signal-connected to the second optical detection component. When the exact position of the target biological particle on the carrier is located by the second optical detection component, the extraction unit is controlled to move to the exact position of the target biological particle, extract the target biological particle, and move the target biological particle to a collection plate.

[0007] Another object of the present invention is to provide a biological particle detection method for detecting and collecting a target biological particle; the biological particle detection method comprises at least the following steps:

[0008] Step S1, providing a biological particle dilution device, the biological particle dilution device comprising a microchannel, a carrier unit and a first optical detection component, the microchannel having a first end and a second end, the first end being connected to a sample source;

[0009] Step S2, the sample source provides a sample including the target biological particles, the carrier unit includes at least one carrier, and the first optical detection component provides a first detection light path, the first detection light path penetrates the microchannel and is used to sense the target biological particles;

[0010] Step S3, when the first optical detection component senses that the target biological particle passes through the microfluidic channel, the carrier unit is controlled to provide the carrier so that the target biological particle passes through the microfluidic channel and the second end and is then carried on the carrier;

[0011] Step S4, providing a detection and extraction device, the detection and extraction device comprising a second optical detection component and an extraction unit, the carrier is controlled to move to the detection and extraction device, and the second optical detection component corresponds to the carrier; the second optical detection component is used to scan, identify and locate the exact position of the target biological particle on the carrier; the extraction unit is signal-connected to the second optical detection component;

[0012] Step S5: When the target biological particle is located at the exact position on the carrier plate by the second optical detection component, the extraction unit is controlled to move to the exact position of the target biological particle, extract the target biological particle, and move the target biological particle to a collection plate.

[0013] The effect of the present invention is that, by using a biological particle detection system with a biological particle dilution device and a detection and extraction device, it can quickly and accurately extract target biological particles, and can avoid the conversion and change of the detection environment causing qualitative changes or contamination of biological samples. By using the biological particle detection method provided by the present invention, a preliminary screening can be performed on trace or rare target biological particles in a large number of samples to reduce the overall volume, weight or composition of the sample, and then the target biological particles in the sample can be located, identified and extracted by precise detection means, and the detection process is carried out in the same detection system, so that the conversion and change of the detection environment can avoid the conversion and change of the biological sample causing qualitative changes or contamination, and avoid the loss of the sample caused by the pre-treatment of the sample, thereby improving the reliability and stability of trace detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 A block diagram of a biological particle detection system according to a preferred embodiment of the present invention.

[0015] Figure 2 Flow chart of a biological particle detection method according to a preferred embodiment of the present invention.

[0016] Figure 3A and Figure 3B Schematic diagram of the relative positions of the first excitation light source and the first light sensor in two preferred embodiments of the present invention.

[0017] Figure 4 FIG. 4 is a diagram showing the relative positions of the second end of the microfluidic channel, the carrier plate and the diluent in a preferred embodiment of the present invention. DETAILED DESCRIPTION

[0018] In order to more clearly illustrate the present invention, preferred embodiments are described in detail below with reference to the accompanying drawings. Figure 1 , Figure 3A , Figure 3B and Figure 4 As shown, Figure 1FIG. 3 is a block diagram of a biological particle detection system 100 according to a preferred embodiment of the present invention; FIG. 4 is a relative position diagram of the first excitation light source and the first light sensor according to a preferred embodiment of the present invention; Figure 4 FIG. 1 is a diagram showing the relative positions of the second end of the microfluidic channel, the carrier plate, and the diluent in a preferred embodiment of the present invention.

[0019] exist Figure 1 , Figure 3A , Figure 3B and Figure 4 In the embodiment, the biological particle detection system 100 is used to detect and collect a target biological particle; the biological particle detection system 100 includes a biological particle dilution device 120 and a detection extraction device 140 .

[0020] The bioparticle dilution device 120 includes a microchannel 122, a carrier unit 124, and a first optical detection assembly 126. The microchannel 122 has a first end 122a and a second end 122b. The first end 122a is connected to a sample source 110. The sample source 110 provides a sample including the target bioparticles.

[0021] The carrier unit 124 includes at least one carrier P. The first optical detection component 126 provides a first detection light path L. The first detection light path L penetrates the microchannel 122 and is used to sense the target biological particles.

[0022] When the first optical detection component 126 senses that the target biological particle passes through the microchannel 122, the carrier unit 124 is controlled to provide the carrier P, so that the target biological particle passes through the microchannel 122 and the second end 122b and is carried on the carrier P. In detail, the sample supply device 130 can controllably regulate the sample of the sample source 110 to enter the first end 122a, so that the flow rate, stop flow and continue flow of the sample in the microchannel 122 are regulated by the sample supply device 130. Therefore, when the first optical detection component 126 senses that the target biological particles pass through the microchannel 122, the sample supply device 130 is adjusted to stop the flow of the sample or reduce the flow speed, and then the carrier unit 124 is controlled to provide the carrier P and move the carrier P to a position corresponding to the opening of the second end 122b. Then, the sample supply device 130 is adjusted to allow the sample to continue to flow so that the target biological particles pass through the second end 122b and are carried on the carrier P.

[0023] The detection and extraction device 140 includes a second optical detection component 142 and an extraction unit 144. The carrier P is moved to the detection and extraction device 140 under control, and the second optical detection component 142 corresponds to the carrier P.

[0024] The second optical detection component 142 is used to scan, identify and locate the exact position of the target biological particles on the carrier P; the extraction unit 144 is signal-connected to the second optical detection component 142. When the exact position of the target biological particles on the carrier P is located by the second optical detection component 142, the extraction unit 144 is controlled to move to the exact position of the target biological particles, extract the target biological particles, and move the target biological particles to a collection plate 150.

[0025] The carrier P can be made of different materials according to user needs, including but not limited to glass and plastic. The bottom surface of the carrier P can be made of transparent material, so that light can penetrate the carrier from the opposite side of the carrier relative to the sample and irradiate the target biological particles, and the radiation light emitted by the target biological particles penetrates the carrier; the bottom surface of the carrier can also be made of opaque material, so that light can directly irradiate the target biological particles from the same side of the carrier relative to the sample.

[0026] In an embodiment of the present invention, the sample supply device 130 can quantitatively, continuously or intermittently regulate the sample of the sample source 110 to enter the first end 122a, so that the flow rate, stop flow and continue flow of the sample in the microchannel 122 are regulated by the sample supply device 130. The sample in the sample source enters the microchannel 122 through the first end 122a and is discharged through the second end 122b. A waste liquid bucket (not shown) is provided for holding the sample discharged from the second end 122b. At the same time, the first optical detection component 126 detects the target biological particles in the sample. When the first optical detection component 126 detects the target biological particles, the sample supply device 130 controls the sample source 110 to controllably stop supplying the sample to the microfluidic channel 122 and enables the disk unit 124 to supply a new disk P to the lower portion of the second end 122b. Then, the sample supply device 130 controls the sample source 110 to controllably continue supplying the sample, so that the sample containing the target biological particles can be discharged into the new disk P. The total volume of the sample discharged into the new disk P is between 1 and 100 μL, so that the operator can change the volume of the sample discharged into the new disk P according to the needs. Then, the disk P containing the target biological particles is moved to the detection and extraction device 140 for extraction. At this time, the first optical detection component 126 will continue to detect the target biological particles until a new biological particle is detected. Then, the above steps are performed again. The disk unit 124 provides a new disk P again, so that the disk P containing the target biological particles enters the detection and extraction device 140.

[0027] like Figure 1 , Figure 3A and Figure 3B As shown, the first optical detection component 126 includes a first excitation light source 126a and a first light sensor 126b, and the first excitation light source 126a and the first light sensor 126b are respectively disposed on planes not parallel to the microchannel 122, so that the first detection light path L passes through the microchannel 122. In one embodiment of the present invention, since the divergence angle of the radiation light of the fluorescent particles bound to the target biological particles after being excited by the laser is 360 degrees, the first light sensor 126b and the first excitation light source 126a are disposed on a plane, and the plane is not parallel to the microchannel 122. In the embodiment of the present invention, the first light sensor 126b includes but is not limited to: a charge coupled device (CCD), a photomultiplier tube (PMT), a high speed charge coupled device (High speed CCD), a complementary metal oxide semiconductor (CMOS) and a scientific complementary metal oxide semiconductor (sCMOS) or a combination thereof.

[0028] The first excitation light source 126a emits excitation light to the first light sensor 126b. During this process, the path of the light forms a first detection light path L. The first detection light path L penetrates the microchannel 122 and is used to sense the target biological particles. In the embodiment of the present invention, when the target biological particles absorb the excitation light emitted by the first excitation light source 126a, they emit a radiation light, which can be fluorescence or cold light.

[0029] The first excitation light source 126a may be a laser, a mercury lamp, or an LED; the wavelength of light emitted by the first excitation light source 126a may be visible light or invisible light.

[0030] In an embodiment of the present invention, the label bound to the target biological particle absorbs the excitation light emitted by the first excitation light source 126a and then emits a radiation light, and the radiation light can be fluorescence or cold light. The label is a molecule that can specifically bind to the biological particle, including but not limited to: anti-EpCAM antibodies, anti-CD45 antibodies, anti-Nucleus antibodies and nucleic acid aptamers (Aptamer), and the label is bonded with a substance that can be excited by light. Therefore, in one embodiment of the present invention, the label is an anti-EpCAM antibody bonded with a fluorescent group. The label can be bound to the biological particles, including but not limited to: deoxyribonucleic acid (DNA), ribonucleic acid (RNA), nuclear proteins, cytoplasmic proteins, cell membrane proteins and cell membrane glycoproteins. In one embodiment of the present invention, after the first excitation light source 126a emits a laser light source with a wavelength of 350 to 700 nm (hereinafter referred to as a light), it excites circulating tumor cells (Circulating tumor A label is provided on a circulating tumor cell (CTC), the label can bind to the EpCAM protein on the membrane of circulating tumor cells, and the label carries Alexa, 488 or PE fluorescent particles. After being excited by laser, the label radiates light with a wavelength of 400 to 800 nm (hereinafter referred to as b light). A filter is arranged at the front end of the first light sensor 126b, so that the light incident on the first light sensor 126b includes: both a and b light will enter the filter. The filter can be selected according to the needs of the user, so that the wavelength of the radiated light emitted after being excited can penetrate, while the other wavelengths cannot penetrate. Therefore, only b light can enter the first light sensor 126b. In one embodiment, the first light sensor 126b is a photomultiplier tube (PMT). After the photomultiplier tube PMT (126b) senses the b light signal, it is determined that the first optical detection component 126 detects the target biological particle.

[0031] like Figure 1 and Figure 4As shown, the biological particle dilution device 120 includes a diluent supply device 128, and the diluent supply device 128 first adds a diluent S to the carrier P. When the first optical detection component 126 detects the target biological particle, the sample supply device 130 controls the sample source 110 to controllably stop supplying the sample to the microchannel 122, causing the second end 122b to stop discharging the sample, and the carrier unit 124 moves the carrier P containing the diluent to below the second end 122b. Then, the sample supply device 130 controls the sample source 110 to controllably continue to supply the sample, so that the sample containing the target biological particle can be discharged into the new carrier P.

[0032] In one embodiment of the present invention, the second end 122b does not contact the diluent surface in the carrier plate P, but is close to the diluent surface. The distance between the second end 122b and the diluent surface allows the sample droplet discharged from the second end 122b to contact the diluent surface. Due to the surface tension between the diluent and the sample liquid, the sample droplet discharged from the second end 122b is immersed in the diluent. The effect of this setting is to prevent the droplet from being affected by the surface tension and resisting gravity and staying at the second end 122b and unable to drip into the carrier plate P smoothly, and to prevent the droplet from remaining on the edge wall of the second end 122b. In one embodiment of the present invention, the distance between the second end 122b and the bottom end of the carrier P is 1 to 20 mm, the liquid level of the diluent S in the carrier P is 0.5 to 10 mm, and the distance between the liquid level of the diluent and the second end is 0.5 to 19.5 mm. The effect of this technical feature is to prevent the discharged droplets D from adhering to the edge wall of the second end 122b and to prevent the droplets D from directly dripping onto the carrier P and causing impact on the biological particles.

[0033] In an experiment of the present invention, a sample containing a cell line was dripped into a carrier plate P at a flow rate of 0.7 mL / min. The experiment was divided into three groups, namely a blank group, an experimental group and a control group. The blank group was a sample that had not undergone any treatment; the experimental group was a sample dripped onto a carrier plate P that had been loaded with a diluent S; the control group was a sample dripped directly onto a carrier plate P that did not contain a diluent S. After dripping 0.7 mL of the sample into the three groups, the activity of the cell line in the sample was tested using the Cell counting kit 8 (CCK8) reagent to analyze whether there were significant differences among the three groups. The results showed that compared with the blank group, the experimental group had no significant difference (P-value was 0.03); compared with the experimental group, the control group had a significant difference (P-value was 0.12). Therefore, directly dripping the sample onto a carrier plate P that did not contain a diluent S would affect the cells. Furthermore, if there is no diluent S during the operation, the liquid containing the biological particles may evaporate due to the long operation time during the user's operation, resulting in the death or loss of activity of the biological particles; on the contrary, if a diluent S is provided, the above problem can be avoided. In the embodiment of the present invention, the carrier P to which the diluent S is added is passed through a homogenizing device 127 to uniformly disperse the target biological particles in the diluent S, thereby achieving the effect of uniformly dispersing the biological particles in the sample, so that the target biological particles and other biological particles are uniformly dispersed. The design of the homogenizing device allows the target biological particles to be uniformly dispersed with other biological particles, so that the density of biological particles in any area on the carrier is similar, which is convenient for detecting and identifying the target biological particles in the biological particles, and at the same time avoiding the absorption of non-target biological particles.

[0034] The second optical detection component 142 includes a second excitation light source 142a, a lens group 142b, a second photomultiplier tube 142c and a charge coupled device 142d. In the embodiment of the present invention, when the target biological particle absorbs the excitation light of the second excitation light source 142a, it emits a radiation light, and the radiation light can be fluorescence or cold light. After the second excitation light source 142a emits the excitation light, it passes through the carrier P and the lens group 142b to the second photomultiplier tube 142c. In this process, the path of the light forms a second detection light path; after the second excitation light source 142a emits the excitation light, it passes through the carrier P and the lens group 142b to the charge coupled device 142d. In this process, the path of the light forms a third detection light path. In the embodiment of the present invention, the light passing through the lens group 142b in the second detection light path includes the excitation light emitted by the second excitation light source 142a and the radiation light. In another embodiment of the present invention, the light passing through the lens group 142b in the third detection light path includes the excitation light emitted by the second excitation light source 142a and the radiated light. In the embodiment of the present invention, the radiated light is incident on the second photomultiplier tube 142c from the second detection light path, and the radiated light is received at the second photomultiplier tube 142c, and the radiated light intensity of the target biological particle is recorded; the radiated light is incident on the charge coupled device 142d from the third detection light path, and the radiated light is received at the charge coupled device 142d, and a radiated light image of the target biological particle is recorded.

[0035] In an embodiment of the present invention, the label bound to the target biological particle emits a radiation light after absorbing the excitation light of the second excitation light source 142a, and the radiation light can be fluorescence or cold light. The label is a molecule that can specifically bind to the biological particle, including but not limited to: anti-EpCAM antibody, anti-CD45 antibody, anti-Nucleus antibody and nucleic acid aptamer (Aptamer), and the label is bonded with a substance that can be excited by light. Therefore, in an embodiment of the present invention, the label is an anti-EpCAM antibody bonded with a fluorescent group. The label can be bound to the biological particle, including but not limited to: DNA, RNA, nuclear protein, cytoplasmic protein, cell membrane protein and cell membrane glycoprotein. In one embodiment of the present invention, the second excitation light source 142a emits a 350-700nm wavelength laser light source (hereinafter referred to as c light) to excite the label on the circulating tumor cells. The label can bind to the EpCAM protein on the circulating tumor cell membrane, and the label carries Alexa, 488 or PE fluorescent particles. After being excited by the laser, the label emits 400-800nm ​​wavelength radiation light (hereinafter referred to as d light). At the same time, both c and d lights will enter the lens group, but there will be a filter in the lens group, so only d light can enter the second photomultiplier tube PMT or charge-coupled device CCD. In another embodiment of the present invention, a second lens group (not shown) is connected to the second excitation light source 142a so that the excitation light enters the second lens group. Therefore, after the second excitation light source 142a emits excitation light, it passes through the second lens group and the carrier P, and then passes through the lens group 142b to the second photomultiplier tube 142c. In this process, the path of light forms a second detection light path; after the second excitation light source 142a emits excitation light, it passes through the second lens group and the carrier P, and then passes through the lens group 142b to the charge coupled device 142d. In this process, the path of light forms a third detection light path. The second lens group may include a filter so that light of a specific wavelength in the light emitted by the second excitation light source 142a can penetrate. The selection of the filter can be selected according to the user's detection requirements to achieve optimal detection efficiency. It should be specially noted that the composition of the lens group 142b and the second lens group lenses and the selection of the magnification can be adjusted according to user needs to meet the detection requirements. Therefore, the second lens group connected to the second excitation light source 142a, the lens group 142b connected to the second photomultiplier tube 142c and the lens group connected to the charge coupled element can be the same lens group or different lens groups.

[0036] Please refer to Figure 1 and Figure 2 , Figure 2The flowchart of a biological particle detection method according to a preferred embodiment of the present invention is shown in FIG. The biological particle detection method is used to detect and collect a target biological particle; the biological particle detection method includes at least the following steps:

[0037] Step S1, providing a biological particle dilution device 120, the biological particle dilution device 120 comprising a microchannel 122, a carrier unit 124 and a first optical detection component 126, the microchannel 122 having a first end 122a and a second end 122b, the first end 122a being connected to a sample source 110;

[0038] Step S2, the sample source 110 provides a sample including the target biological particles, the carrier unit 124 includes at least one carrier P, and the first optical detection component 126 provides a first detection light path, which is a path for light to travel from the first excitation light source 126a through the microchannel 122 to the first light sensor 126b, and is used to sense the target biological particles;

[0039] Step S3, when the first optical detection component 126 senses that the target biological particle passes through the microchannel 122, the carrier unit 124 is controlled to provide the carrier P, so that the target biological particle passes through the microchannel 122 and the second end 122b and is then carried on the carrier P;

[0040] Step S4, providing a detection and extraction device 140, the detection and extraction device 140 includes a second optical detection component 142 and an extraction unit 144, the carrier P is controlled to move to the detection and extraction device 140, and the second optical detection component 142 corresponds to the carrier P; the second optical detection component 142 is used to scan, identify and locate the exact position of the target biological particle on the carrier; the extraction unit 144 is connected to the second optical detection component by signal;

[0041] Step S5: When the target biological particle is located at the exact position on the carrier plate by the second optical detection component 142, the extraction unit 144 is controlled to move to the exact position of the target biological particle, extract the target biological particle, and move the target biological particle to a collection plate.

[0042] The first optical detection component 126 includes a first excitation light source 126 a and a first light sensor 126 b , and the first excitation light source 126 a and the first light sensor 126 b are respectively disposed on planes that are not parallel to the microchannel 122 , so that the first detection light path passes through the microchannel 122 .

[0043] The biological particle dilution device 120 includes a diluent supply device 128, and the diluent supply device 128 first adds a diluent to the carrier P, and then discharges the target biological particles from the second end 122b of the microchannel 122 and places them on the carrier P. In the embodiment of the present invention, the carrier P to which the diluent is added passes through a homogenizing device 127, so that the target biological particles are evenly dispersed in the diluent.

[0044] The second optical detection component 142 includes a second excitation light source 142a, a lens group 142b, a second photomultiplier tube 142c and a charge-coupled device 142d. The second excitation light source 142a generates a second detection light path and a third detection light path. The second detection light path is emitted from the second excitation light source 142a and passes through the carrier P and the lens group 142b to the second photomultiplier tube 142c. The third detection light path is emitted from the second excitation light source 142a and passes through the carrier P and the lens group 142b to the charge-coupled device 142d. In an embodiment of the present invention, when the target biological particle absorbs the excitation light of the second excitation light source 142a, it emits a radiation light, and the radiation light can be fluorescence or cold light. The radiation light is incident on the second photomultiplier tube 142c from the second detection light path, and is received by the second photomultiplier tube 142c, and the radiation light intensity of the target biological particle is recorded; the radiation light is incident on the charge-coupled element 142d from the third detection light path, and is received by the charge-coupled element 142d, and a radiation light image of the target biological particle is recorded.

[0045] In the above-mentioned embodiment of the present invention, the biological particle detection system using the biological particle dilution device and the detection and extraction device can quickly and accurately extract the target biological particles, and can avoid the conversion and change of the detection environment causing the biological sample to be qualitatively changed or contaminated. The biological particle dilution device can quickly and preliminarily determine the samples that may contain the target biological particles and exclude the samples that do not contain the target biological particles. Then, the samples that may contain the target biological particles are passed through the detection and extraction device to accurately locate, identify and extract the target biological particles. The detection process is all carried out in the same detection system, so it can avoid the conversion and change of the detection environment causing the biological sample to be qualitatively changed or contaminated, thereby improving the reliability and stability of trace detection.

[0046] The above description is only a preferred embodiment of the present invention. Any equivalent changes made by applying the present specification and the scope of the patent application should be included in the patent scope of the present invention.

[0047] Description of Reference Numerals

[0048] [The present invention]

[0049] 100: Biological particle detection system

[0050] 110: Sample source

[0051] 120: Biological particle dilution device

[0052] 122: Microfluidics

[0053] 122a: First end

[0054] 122b: Second end

[0055] 124: Plate loading unit

[0056] 126: First optical detection component

[0057] 126a: First excitation light source

[0058] 126b: First light sensor

[0059] 127: Homogenization device

[0060] 128: Dilution liquid supply device

[0061] 130: Sample supply device

[0062] 140: Detection of extraction device

[0063] 142: Second optical detection component

[0064] 142a: Second excitation light source

[0065] 142b: Lens group

[0066] 142c: Second photomultiplier tube

[0067] 142d: Charge-coupled device

[0068] 144: Extraction Unit

[0069] 150: Collection Plate

[0070] D: Droplet

[0071] L: First detection optical path

[0072] P: Carrier

[0073] S: diluent

Claims

1. A biological particle detection system for detecting and collecting a target biological particle; the biological particle detection system comprises: A biological particle dilution device comprises a microfluidic channel, a carrier unit and a first optical detection component, wherein the microfluidic channel has a first end and a second end, the first end is connected to a sample source, the sample source provides a sample containing the target biological particle, the first optical detection component provides a first detection light path, the first detection light path penetrates the microfluidic channel and is used to sense the target biological particle; the carrier unit comprises at least one carrier for holding the target biological particle; A detection and extraction device includes a second optical detection component and a extraction unit. The carrier is controlled to move to the detection and extraction device, and the second optical detection component corresponds to the carrier. The second optical detection component locates the exact position of the target biological particle on the carrier. The extraction unit is signal-connected to the second optical detection component. When the exact position of the target biological particle on the carrier is located by the second optical detection component, the extraction unit is controlled to move to the exact position of the target biological particle, extract the target biological particle, and move the target biological particle to a collection plate.

2. The biological particle detection system according to claim 1, wherein: The first optical detection component includes a first excitation light source and a first light sensor, and the first excitation light source and the first light sensor are respectively arranged on planes that are not parallel to the microchannel, so that the first detection light path passes through the microchannel.

3. The biological particle detection system according to claim 1, wherein: The biological particle dilution device includes a diluent supply device, which first adds a diluent into the carrier, and then discharges the target biological particles from the second end of the microchannel and places them on the carrier.

4. The biological particle detection system according to claim 3, wherein: The carrier plate to which the diluent is added is passed through a homogenizing device to uniformly disperse the target biological particles in the diluent.

5. The biological particle detection system according to claim 1, wherein: The second optical detection component includes a second excitation light source, a lens group, a second photomultiplier tube and a charge-coupled device. The second excitation light source generates a second detection light path and a third detection light path. The second detection light path is emitted from the second excitation light source and passes through the carrier and the lens group to the second photomultiplier tube, while the third detection light path is emitted from the second excitation light source and passes through the carrier and the lens group to the charge-coupled device.

6. The biological particle detection system according to claim 5, wherein: When the target biological particle absorbs the excitation light of the second excitation light source, it emits a radiation light, and the radiation light is emitted into the charge coupled device through the third detection light path, and is received by the charge coupled device, and a radiation light image of the target biological particle is recorded.

7. A biological particle detection method for detecting and collecting a target biological particle; the biological particle detection method comprises: A biological particle dilution device is provided, the biological particle dilution device comprising a microfluidic channel, a carrier unit and a first optical detection component, the microfluidic channel has a first end and a second end, the first end is connected to a sample source; The sample source provides a sample including the target biological particles, the carrier unit includes at least one carrier, the at least one carrier holds the sample discharged through the second end, the first optical detection component provides a first detection light path, the first detection light path penetrates the microchannel and is used to sense the target biological particles; When the first optical detection component senses that the target biological particle passes through the microfluidic channel, the carrier unit is controlled to provide the at least one carrier so that the target biological particle passes through the microfluidic channel and the second end and is carried on the at least one carrier; A detection and extraction device is provided, the detection and extraction device comprises a second optical detection component and an extraction unit, the carrier is controlled to move to the detection and extraction device, and the second optical detection component corresponds to the carrier; the second optical detection component is used to scan, identify and locate the exact position of the target biological particle on the carrier; the extraction unit is connected to the second optical detection component by signal; When the target biological particle is located at the exact position on the carrier plate by the second optical detection component, the extraction unit is controlled to move to the exact position of the target biological particle, extract the target biological particle, and move the target biological particle to a collection plate.

8. The biological particle detection method according to claim 7, wherein: The first optical detection component includes a first excitation light source and a first light sensor, and the first excitation light source and the first light sensor are respectively arranged on planes that are not parallel to the microchannel, so that the first detection light path passes through the microchannel.

9. The biological particle detection method according to claim 7, wherein: The biological particle dilution device includes a diluent supply device, which first adds a diluent into the carrier, and then discharges the target biological particles from the second end of the microchannel and places them on the carrier.

10. The biological particle detection method according to claim 9, wherein: The carrier plate to which the diluent is added is passed through a homogenizing device to uniformly disperse the target biological particles in the diluent.

11. The biological particle detection method according to claim 7, wherein: The second optical detection component includes a second excitation light source, a lens group, a second photomultiplier tube and a charge-coupled device. The second excitation light source generates a second detection light path and a third detection light path. The second detection light path is emitted from the second excitation light source and passes through the carrier and the lens group to the second photomultiplier tube, while the third detection light path is emitted from the second excitation light source and passes through the carrier and the lens group to the charge-coupled device.

12. The biological particle detection method according to claim 11, wherein: When the target biological particle absorbs the excitation light of the second excitation light source, it emits a radiation light, and the radiation light is emitted into the charge coupled device through the third detection light path, and is received by the charge coupled device, and a radiation light image of the target biological particle is recorded.

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