POCT blood cell analyzer and its use method

Abstract

The present application provides a POCT blood cell analyzer and a method for using the same. The POCT blood cell analyzer includes a housing, a detection seat, and a pipette. The detection seat can be extended into or out of the housing to receive a reagent kit provided with an impedance detection pool. The detection seat is provided with a power supply for cooperating with impedance detection. The reagent kit has an installation head accommodating pool, which is used to install the installation head. The pipette is arranged in the housing and above the detection seat. The pipette is used to load the installation head for performing corresponding operations. When the installation head needs to be unloaded, the pipette moves to a preset position outside the installation head accommodating pool to perform the installation head unloading operation.

Description

Technical Field

[0001] The present application relates to the field of medical device technology, and in particular to a POCT blood cell analyzer, a pipette, a detection base, a method for using the POCT blood cell analyzer, a test kit, an assembly base, a sample detection device, and a microporous sheet. Background Art

[0002] Hematology analyzers, also known as hematology analyzers, hematology meters, and blood cell counters, are among the most widely used instruments in hospitals for clinical testing. A significant portion of the components within traditional hematology analyzers are part of the cleaning system, as traces of the previous sample tube must be cleaned before the next one is tested. This cleaning system is not only complex and complex, with numerous components, but the cleaning process also requires a large amount of reagents and is time-consuming.

[0003] Compared with traditional blood cell analyzers, POCT blood cell analyzers have made great simplifications in instrument components. POCT blood cell analyzers can completely remove the cleaning fluid path-related components in traditional blood analysis, greatly reducing the complexity and production cost of the instrument.

[0004] However, some existing POCT blood cell analyzers have overly simple functions, some have relatively complex structures, some have low levels of automation, and some are relatively expensive. Summary of the Invention

[0005] The present application provides a POCT blood cell analyzer, a pipette, a detection base, a method for using the POCT blood cell analyzer, a test kit, an assembly base, a sample detection device, and a microporous sheet to at least partially solve the above-mentioned technical problems.

[0006] In order to solve the above technical problems, a technical solution adopted by the present application is to provide a POCT blood cell analyzer, which includes:

[0007] case;

[0008] A detection seat, which can be extended into or out of the housing, is used to receive a reagent kit provided with an impedance detection pool. The detection seat is provided with a power supply for impedance detection. The reagent kit has a mounting head accommodating pool, and the mounting head accommodating pool is used to install the mounting head.

[0009] A pipette is provided in the shell and located above the detection seat. The pipette is used to load the mounting head to perform corresponding operations. When the mounting head needs to be unloaded, the pipette moves to a preset position outside the mounting head accommodating pool to perform the mounting head unloading operation.

[0010] The beneficial effects of the present application are: different from the existing technology, the present application provides a POCT blood cell analyzer, a pipette, a detection base, a method of using the POCT blood cell analyzer, a test kit, an assembly base, a sample detection device, and a microporous sheet, which are novel in structure, practical and reliable, low in cost, and can efficiently complete fully automated detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to more clearly illustrate the technical solutions in this embodiment, the following briefly introduces the drawings required for describing the embodiment. Obviously, the drawings described below are only some embodiments of the present application. Those skilled in the art can also derive other drawings based on these drawings without inventive work, among which:

[0012] Figure 1 This is a schematic diagram of the three-dimensional structure of a POCT blood cell analyzer provided in one embodiment of the present application;

[0013] Figure 2 This is a schematic diagram of the module structure of a POCT blood cell analyzer provided in one embodiment of the present application;

[0014] Figure 3 This is a schematic diagram of the internal structure of a POCT blood cell analyzer provided in one embodiment of the present application;

[0015] Figure 4 This is a schematic diagram of the internal structure of a POCT blood cell analyzer provided in one embodiment of the present application, wherein the pipette is omitted;

[0016] Figure 5 This is a schematic diagram of the gas circuit structure of the pressure building system provided in one embodiment of the present application;

[0017] Figure 6 This is a schematic diagram of the three-dimensional structure of a pipette provided in one embodiment of the present application;

[0018] Figure 7 This is a schematic diagram of the three-dimensional structure of a puncture head provided in one embodiment of the present application;

[0019] Figure 8 This is a bottom-up structural schematic diagram of a metal shielding cover and a transmission mechanism of a POCT blood cell analyzer provided in one embodiment of the present application;

[0020] Figure 9 This is a schematic diagram of the three-dimensional structure of the detection base of the POCT blood cell analyzer provided in one embodiment of the present application;

[0021] Figure 10 This is a schematic diagram of the exploded structure of the detection base of the POCT blood cell analyzer provided in one embodiment of the present application;

[0022] Figure 11 This is a side structural diagram of the conductive support and conductive column of a POCT blood cell analyzer provided by another embodiment of the present application;

[0023] Figure 12 1 is a schematic diagram of a top view of a kit provided in one embodiment of the present application;

[0024] Figure 13 This is a simplified three-dimensional perspective structural diagram of a POCT blood cell analyzer provided in another embodiment of the present application;

[0025] Figure 14 This is a flowchart of the use of a POCT blood cell analyzer provided in one embodiment of the present application;

[0026] Figure 15 This is a schematic diagram of the exploded structure of a test kit provided in one embodiment of the present application from one perspective;

[0027] Figure 16 This is a schematic diagram of the exploded structure of the kit provided in one embodiment of the present application from another perspective;

[0028] Figure 17 This is a schematic cross-sectional structural diagram of a kit provided in one embodiment of the present application from one perspective;

[0029] Figure 18 This is a schematic cross-sectional structural diagram of a kit provided in one embodiment of the present application from another perspective;

[0030] Figure 19 Schematic diagram of the three-dimensional structure of a microporous sheet provided in one embodiment of the present application;

[0031] Figure 20 yes Figure 19 Schematic diagram of the cross-sectional structure of the microporous sheet shown in . DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0033] It should be noted that if the embodiments of the present application involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0034] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present application, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the fact that they can be implemented by ordinary technicians in this field. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by this application.

[0035] The present application provides a POCT blood cell analyzer and a method for using the same.

[0036] like Figures 1 to 14 As shown, the POCT blood cell analyzer includes a housing 10, a display screen 11, an outlet 12, a syringe 220, a frame 301, a pressure building system, a detection seat 300 and its transmission mechanism, a pipette 400 and its transmission mechanism, a metal shielding cover 500 and its transmission mechanism, etc.

[0037] The detection seat 300 can reciprocate in and out of the port 12 to facilitate the loading or unloading of the reagent kit 20. The pipette 400 and its transmission mechanism are used to realize the fully automated pre-processing of the sample to be tested (which can be pre-installed on the reagent kit 20). The fully automated pre-processing includes automatic sample addition, automatic reagent addition (the reagent kit 20 is pre-installed with diluent, hemolytic agent, staining and lysis reagent, etc.), automatic mixing (bubbling or stirring), etc. The pressure building system is used to provide the positive and negative pressure required for the fully automated pre-processing of the pipette 400. The metal shielding cover 500 and its transmission mechanism are used to realize the fully automated pre-processing of the sample to be tested (which can be pre-installed on the reagent kit 20). The structure is used to cooperate with the pressure-building system to perform automatic testing. Automatic testing means that when the metal shielding cover 500 and the detection seat 300 are covered to form an electromagnetic shielding space, the air pressure connection device 510 of the pressure-building system is connected to the pressure chamber 140 on the test kit 20 (the pressure chamber 140 is connected to the rear tank of the impedance detection tank). The pressure chamber 210 of the pressure-building system begins to provide negative or positive pressure, causing the liquid in the front tank 120 of the impedance detection tank of the test kit 20 to flow through the micropores to the rear tank of the impedance detection tank of the test kit 20, and relevant parameters are recorded during this process. Among them, the air pressure connection device 510 of the pressure-building system is connected to the metal shielding cover 500 and moves synchronously.

[0038] The pressure building system includes a pressure chamber 210, a first syringe 222 and a second syringe 223 that are linked together, a driving member 224, an air filter 225, a first solenoid valve SV1, a second solenoid valve SV2, a third solenoid valve SV3, an air pressure connection device 510, etc.

[0039] The pipette 400 includes an air tube 401, an outer sleeve 402 mounted on the outer periphery of the air tube 401, a synchronization block 403, a light shield 404, an optical coupler 405, a fixing plate 406, a through slot 407, and a blocking member 4031 for unloading the mounting head (201 or 204).

[0040] The metal shielding cover 500 is installed on the frame 301 through the mounting frame 520, and is lifted and lowered in the vertical direction by the motor 530 and the guide column 540. The metal shielding cover 500 is in the shape of a cover with an opening at the bottom, and the air pressure connection device 510 connected to the pressure building system extends into the interior of the metal shielding cover 500.

[0041] The detection base 300 includes a main body 310, a conductive support 303 connected to the main body 310, a metal shielding base (320, 321, 322) covering the main body 310, an optical detection component 330 embedded in the main body 310, a Peltier 340 arranged in sequence near the detection cavity of the optical detection component 330, a heat sink 350 and a fan 360, etc.

[0042] The following will provide a detailed description based on various embodiments of the POCT blood cell analyzer.

[0043] The first embodiment, as Figures 1 to 13 As shown, this embodiment provides a POCT blood cell analyzer, which includes a housing 10 , a detection seat 300 , and a pipette 400 .

[0044] The detection seat 300 can be extended into or out of the housing 10 to receive the reagent kit 20 provided with the impedance detection cell. The detection seat 300 is provided with a power supply (eg Figure 9 The reagent kit 20 has a mounting head accommodating pool for accommodating a mounting head (tip head 201 or puncture head 204).

[0045] The pipette 400 is arranged in the housing 10 and is located above the detection seat 300, and is used to perform corresponding operations on the reagent kit 20, including pipetting, bubbling and mixing, stirring and mixing, and piercing the sealing film. When the mounting head needs to be unloaded (including normal detection completion and accidental power failure), the pipette 400 moves to a preset position outside the mounting head accommodating pool (for example, Figure 12 Performing the mounting head unloading operation using the front pool 120 in the apparatus can ensure that the pipette 400 will not place the mounting head again when the mounting head holding pool is already filled with a mounting head in the event of an unexpected power outage and restart. It can also shorten the running path when unloading the mounting head and reduce the alignment requirements when unloading the mounting head (generally, the diameter of the mounting head holding pool is relatively small, and precise alignment is required during unloading).

[0046] This embodiment also provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, a pipette 400 and a detector (which may be a detection element such as an optical coupler 405).

[0047] The test base 300 can be extended into or out of the housing 10 to receive the reagent kit 20 with an impedance detection pool. The test base 300 is provided with a power supply for impedance detection. The reagent kit 20 has an installation head accommodating pool for installing the installation head.

[0048] The pipette 400 is disposed in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent kit 20 .

[0049] The detector is used to detect whether the pipette 400 is loaded with a mounting head when the POCT hematology analyzer needs to unload the mounting head. If it is detected that the pipette 400 is loaded with a mounting head, the pipette 400 moves to the mounting head accommodating pool or a preset position outside to perform the mounting head unloading operation. By setting up the detector, it is possible to know whether the pipette 400 is loaded with a mounting head and then selectively perform the unloading operation, which is relatively more intelligent.

[0050] This embodiment also provides a POCT blood cell analyzer, which is used to receive a reagent kit 20 and includes a detection seat 300 and a pipette 400.

[0051] The test base 300 is used to receive the test kit 20 with an impedance detection pool, and the test base 300 is provided with a power supply for impedance detection. The test kit 20 has an installation head accommodating pool, which is used to install the installation head.

[0052] The pipette 400 is arranged above the detection seat 300 and is used to load the mounting head to perform corresponding operations on the reagent kit 20. When the mounting head needs to be unloaded, the pipette 400 moves to the mounting head accommodating pool or a preset position outside the mounting head accommodating pool to perform the mounting head unloading operation. In this embodiment, the POCT hematology analyzer also includes a detector (which can be a detection element such as an optical coupler 405). The detector is used to detect whether the mounting head is loaded on the pipette 400 when the POCT hematology analyzer needs to unload the mounting head. If it is detected that the pipette 400 is loaded with a mounting head, the pipette 400 unloads the mounting head to the mounting head accommodating pool or a preset position outside the mounting head accommodating pool. The operation of the pipette 400 unloading the mounting head to the preset position outside the mounting head accommodating pool is simpler, that is, directly finding a large-caliber pool body nearby for unloading, which can avoid repeatedly placing the mounting head when the mounting head accommodating pool is already equipped with a mounting head, and can also shorten the running path when unloading the mounting head, while reducing the alignment requirements when unloading the mounting head.

[0053] The reagent kit 20 has multiple cells, one of which is located in the preset position. These cells include a mounting head storage tank that matches the mounting head and several functional tanks. The pipette 400 unloads the mounting head into any of the functional tanks. The functional tanks may include the diluent tank 111 and the forecell 120 (either the WBC detection tank or the RBC detection tank). Unloading the mounting head into any of the functional tanks by the pipette 400 effectively selects a completely empty tank position with a relatively large opening.

[0054] like Figure 6 As shown, the pipette 400 includes an air guide tube 401 and an outer sleeve 402 . When the outer sleeve 402 moves axially relative to the air guide tube 401 , the mounting head can be unloaded.

[0055] This embodiment also provides a method for using a POCT blood cell analyzer, the method comprising:

[0056] The pipette 400 of the POCT blood cell analyzer moves to the mounting head receiving tank to load the mounting head for performing the relevant operations of the first detection;

[0057] The first test can be completed normally or unexpectedly loses power. Before the second test is performed, the mounting head used in the first test needs to be removed. The pipette 400 of the POCT hematology analyzer moves to a preset position outside the mounting head reservoir to perform the head removal operation. This method ensures that the pipette 400 does not place a mounting head in the reservoir if one is already installed. It also shortens the travel path when removing the mounting head and reduces alignment requirements during removal.

[0058] This embodiment also provides a method for using a POCT blood cell analyzer, the method comprising:

[0059] The pipette 400 of the POCT blood cell analyzer moves to the mounting head receiving tank to load the mounting head for performing the relevant operations of the first detection;

[0060] The first test may be completed normally or unexpectedly powered off. Before the second test is performed, the mounting head used in the first test needs to be removed. A detector can be used to detect whether the mounting head is loaded on the pipette 400 of the POCT hematology analyzer.

[0061] If it is detected that a mounting head is loaded on the pipette 400 , the pipette 400 moves to a preset position outside the mounting head accommodation pool to perform a mounting head unloading operation.

[0062] This usage method can determine whether the pipette 400 is loaded with a mounting head and then selectively perform an unloading operation, which is relatively more intelligent.

[0063] This embodiment also provides a method for using a POCT blood cell analyzer, including:

[0064] The pipette of the POCT hematology analyzer moves to the mounting head receiving pool to load the mounting head for performing related operations for the first test;

[0065] The first test may be completed normally or in the event of an unexpected power outage. Before the second test is performed, the reagent kit 20 and the mounting head used in the first test need to be removed. Therefore, it is necessary to check whether the POCT blood cell analyzer is equipped with the reagent kit 20.

[0066] Before the second test is performed, a detector may be used to detect whether the pipette 400 of the POCT hematology analyzer is loaded with a mounting head;

[0067] If it is detected that the POCT hematology analyzer is equipped with a reagent kit 20 and that a mounting head is loaded on the pipette 400, the pipette 400 is controlled to move to the mounting head receiving pool or a preset position outside the mounting head receiving pool to perform the mounting head unloading operation. When the mounting head unloading operation is performed at a preset position outside the mounting head receiving pool, the mounting head is not repeatedly placed if the mounting head is already loaded in the mounting head receiving pool. This can also shorten the travel path when unloading the mounting head and reduce the alignment requirements when unloading the mounting head.

[0068] The preset position may correspond to the interior of the detection base 300 or to the exterior of the detection base 300. In other words, the unloading operation is not limited to unloading the mounting head onto the detection base 300 or onto the reagent cartridge 20. The mounting head may also be unloaded directly to an area inside the instrument other than the detection base 300. For example, a mounting head recovery box may be provided inside the instrument, and maintenance personnel may regularly empty the mounting head recovery box.

[0069] The pipette 400's outer sleeve 402 can be axially moved relative to the pipette's air guide tube 401 to unload the mounting head. In a specific embodiment, the pipette 400 unloads the mounting head via a fixed or retractable blocking member 4031. The blocking member 4031 can be a telescopic motor, a vertically or horizontally mounted electromagnet, or a U-shaped baffle. A synchronization block 403 can be connected to the outer sleeve 402. When the blocking member 4031 blocks the synchronization block 403 and the pipette 400 rises, the mounting head is unloaded from the lower end of the outer sleeve 402.

[0070] Please refer to the second embodiment. Figures 1 to 13 , this embodiment provides a pipette 400, which includes an air guide tube 401 and a detection component.

[0071] The air guide tube 401 is used to load the mounting head (tip head 201 or puncture head 204 ), and the detection component is provided on the side of the air guide tube 401 for detecting whether the mounting head is loaded on the air guide tube 401 .

[0072] The pipette 400 also includes an outer sleeve 402, which is sleeved on the outer circumference of the air guide tube 401. When the air guide tube 401 is loaded with the mounting head, the air guide tube 401 is inserted into the mounting head so that the mounting head pushes the outer sleeve 402 to change its position along the axial direction of the air guide tube 401. The detection part is used to detect the position of the outer sleeve 402 and then determine whether the mounting head is sleeved on the air guide tube 401.

[0073] When the mounting head is sleeved on the air guide tube 401, the outer sleeve 402 is located at the first position of the air guide tube 401 ( Figure 6 When the mounting head is not sleeved on the air guide tube 401 , the outer sleeve 402 is located at the second position of the air guide tube 401 , and the first position is higher than the second position.

[0074] A light blocking piece 404 is connected to the outer sleeve 402, and the detection part is an optical coupler 405 that is matched with the light blocking piece 404. When the optical channel of the optical coupler 405 is blocked by the light blocking piece 404, it means that a mounting head is connected to the air guide tube 401. When the optical channel of the optical coupler 405 is not blocked by the light blocking piece 404, it means that no mounting head is connected to the air guide tube 401.

[0075] The pipette 400 includes a fixing plate 406 disposed on the side of the air guide tube 401, and the optical coupler is disposed on the fixing plate 406. The air guide tube 401 of the pipette 400 can move up and down independently, and the pipette 400 as a whole can move in two or three dimensions.

[0076] The fixing plate 406 is provided with a through slot 407, and a pair of optical transceiver parts of the optical coupler 405 extend into the through hole and are located on the side of the air guide tube 401. The outer sleeve 402 is also connected to a synchronization block 403, and the synchronization block 403 extends into the through slot 407. When the air guide tube 401 rises and the synchronization block 403 is blocked, the mounting head is unloaded. After the mounting head is unloaded, the outer sleeve 402 is still sleeved on the outer periphery of the air guide tube 401, and because the synchronization block 403 extends into the through slot 407, the outer sleeve 402 will not fall off relative to the air guide tube 401, but is naturally supported at the bottom of the through slot 407 (that is, the second position).

[0077] This embodiment also provides a method for using a POCT blood cell analyzer, comprising the following steps:

[0078] Before performing pipetting, it is determined whether the air guide tube 401 of the pipette 400 is equipped with a mounting head;

[0079] If the pipette 400's air tube 401 is not fitted with a mounting head, an alarm is issued. Since the piping in a POCT hematology analyzer is airflow, not liquid flow, and liquid cannot be aspirated through the piping, the air tube 401 must be fitted with a mounting head (tip 201) to operate. Therefore, it is necessary to detect whether the pipette 400's air tube 401 is fitted with a mounting head.

[0080] In the step of detecting whether the air tube 401 of the pipette 400 is equipped with a mounting head, an outer sleeve 402 is provided on the outer periphery of the air tube 401 , and the position of the outer sleeve 402 is detected by the detection part to determine whether the air tube 401 is equipped with a mounting head.

[0081] When the mounting head is sleeved on the air guide tube 401, the outer sleeve 402 is located at the first position of the air guide tube 401. When the mounting head is not sleeved on the air guide tube 401, the outer sleeve 402 is located at the second position of the air guide tube 401. The first position is higher than the second position.

[0082] The detection component is an optical coupler 405, and the outer sleeve 402 is provided with a light shield 404 that matches the optical coupler 405. When the light shield 404 blocks the light channel of the optical coupler 405, it is determined that the mounting head outer sleeve 402 is sleeved on the air guide tube 401.

[0083] This embodiment provides a POCT blood cell analyzer, including a housing 10 , a detection seat 300 , a pipette 400 , and a detection element.

[0084] The detection seat 300 can be extended into or out of the shell 10, and is used to receive the reagent kit 20 equipped with an impedance detection cell; the pipette 400 is arranged in the shell 10 and is located above the detection seat 300. The pipette 400 includes an air guide tube 401, which is used to load the installation head (tip head 201 or puncture head 204); the detection component is arranged on the side of the air guide tube 401, and is used to detect whether the installation head is loaded on the air guide tube 401. The detection component can be an optical coupler 405 or other detection element that can detect the presence or absence of an object.

[0085] In this embodiment, the detection element is provided to detect the loading status of the mounting head, thereby preventing the pipette from performing a pipetting operation when the mounting head is not loaded and contaminating the internal air path of the POCT blood cell analyzer.

[0086] Please refer to the third embodiment. Figures 1 to 13This embodiment provides a POCT blood cell analyzer, which includes a detection seat 300, a pipette 400 arranged above the detection seat 300, and a pressure building system. The detection seat 300 is used to receive a reagent kit 20. The reagent kit 20 is provided with a front pool 120 and a rear pool connected by micropores. The pressure building system includes a pressure chamber 210, an air pressure connection device 510, a first syringe 222, a second syringe 223, and a driving member 224.

[0087] The first syringe 222 is connected to the pressure chamber 210 or the pipette 400, and is used to establish positive pressure or negative pressure in the pressure chamber 210. It is also used to assist the pipette 400 in pipetting, or to bubble and mix the liquid in the front pool 120. The second syringe 223 is connected to the pipette 400, and is used to aspirate samples and reagents. The driving member 224 is used to simultaneously drive the first syringe 222 and the second syringe 223. The first syringe 222 and the second syringe 223 can be linked syringes, which are convenient for sharing the same driving member 224. The driving member 224 can be a motor. Of course, the first syringe 222 and the second syringe 223 can also be separate syringes and are independently controlled by different motors.

[0088] The POCT blood cell analyzer further includes solenoid valves, which include a first solenoid valve SV1, a second solenoid valve SV2, and a third solenoid valve SV3.

[0089] The first solenoid valve SV1 selectively connects the first syringe 222 with the pressure chamber 210 and the second syringe 223; the second solenoid valve SV2 selectively connects the first solenoid valve SV1 with the pressure chamber 210 and the outside atmosphere, and the outside atmosphere is connected to the second solenoid valve SV2 through the air filter 225 and the pipeline T10; the third solenoid valve SV3 selectively connects the air pressure connecting device 510 with the pressure chamber 210, and when the third solenoid valve SV3 is energized, the pipelines T8 and T7 are connected through the third solenoid valve SV3.

[0090] The first syringe 222 can inhale air through the air filter 225, T10 pipeline, the second solenoid valve SV2, T5 pipeline, the first solenoid valve SV1, and T1 pipeline, and then establish positive pressure to the pressure chamber 210 through the T1 pipeline, the first solenoid valve SV1, T5 pipeline, the second solenoid valve SV2, and T6 pipeline.

[0091] The first syringe 222 can suck away the gas in the pressure chamber 210 through the T1 pipeline, the first solenoid valve SV1, the T5 pipeline, the second solenoid valve SV2, and the T6 pipeline to establish a negative pressure, and then exhaust the gas through the T1 pipeline, the first solenoid valve SV1, the T5 pipeline, the second solenoid valve SV2, the T10 pipeline, and the air filter 225.

[0092] After establishing positive or negative pressure, pressure chamber 210 can output the positive or negative pressure through pipeline T7, third solenoid valve SV3, pipeline T8, and air pressure connection device 510. Pipe T8 can be relatively rigid, meaning its rigidity is greater than that of second conduit 221. This greater rigidity prevents the walls of pipeline T8 from abutting against each other, blocking the internal airflow path of pipeline T8, when negative pressure is output.

[0093] When the first solenoid valve SV1 connects the T1 pipeline with the T2 pipeline, the positive pressure or negative pressure generated by the first syringe 222 and the second syringe 223 acts on the pipette 400 at the same time, that is, when a large amount of sample is required to be sucked or pushed, the first syringe 222 can play a role in assisting pipetting.

[0094] The volume of the first syringe 222 is greater than that of the second syringe 223. The first syringe 222 is mainly used for building pressure, and the second syringe 223 is mainly used for pushing / sucking samples. Of course, both syringes can also be used simultaneously for building pressure or pushing / sucking samples as needed.

[0095] The volume ratio of the first syringe 222 to the second syringe 223 can be 80 to 120:1. For example, the volume of the first syringe 222 is 10 milliliters, and the volume of the second syringe 223 is 100 microliters. The driving member 224 drives the piston rods of the first and second syringes 222, 223 via the linkage plate 231, causing the first and second syringes 222, 223 to move synchronously. The piston rod of the second syringe 223 is in sealed engagement with the rear end of the outer barrel of the second syringe 223.

[0096] The POCT blood cell analyzer further includes a housing 10 and a frame 301 . The pressure chamber 210 is disposed on the housing 10 or on the frame 301 . The pipette 400 , the first syringe 222 , the second syringe 223 , and the driving member are disposed on the frame 301 .

[0097] The detection seat 300 is slidably or rotatably arranged relative to the frame 301 and is located below the pipette 400 .

[0098] The POCT blood cell analyzer also includes a metal shielding cover 500, and the detection seat 300 can move relative to the frame 301 to the liquid preparation position (i.e. Figure 3 The detection base 300 can be moved relative to the frame 301 to a sample detection station (not shown in the figure, corresponding to directly below the metal shielding cover 500). The metal shielding cover 500 is used to lower at the sample detection station to cooperate with the detection base 300 to form a relatively closed metal cavity, thereby shielding external electromagnetic interference. The frame 301 can have a two-layer structure, with the detection base 300 positioned on the lower layer and the pipette 400, metal shielding cover 500, and syringe 220 positioned on the upper layer.

[0099] like Figure 1 As shown, the housing 10 is provided with a display screen 11 and an outlet 12, and the detection seat 300 is used to move to the outlet 12 to receive the reagent kit 20 and load it into the detection seat 300. The distance between the liquid preparation station and the outlet 12 is less than the distance between the sample detection station and the outlet 12. In other words, the outlet 12 faces the interior of the instrument, which is the liquid preparation station and the sample detection station. The detection seat 300 reciprocates between the loading station, the liquid preparation station and the sample detection station, and the liquid preparation station is located between the loading station and the sample detection station. In other embodiments, the liquid preparation station and the sample detection station can also be the same station.

[0100] This embodiment also provides a method for using the aforementioned POCT blood cell analyzer, comprising the following steps:

[0101] Controlling the movement of the driving member 224 to establish positive pressure or negative pressure in the pressure chamber 210 through the first syringe 222;

[0102] Controlling the movement of the driving member 224 to perform pipetting through the second syringe 223 and the pipette 400;

[0103] The pressure release of the pressure chamber 210 is controlled to apply positive pressure to the front pool 120 or negative pressure to the rear pool through the air pressure connection device 510 to enable the liquid in the front pool 120 to flow to the rear pool through the micropores.

[0104] The POCT blood cell analyzer provided in this embodiment has a reasonable layout, a compact structure, and is easy to use.

[0105] Please refer to the fourth embodiment Figures 1 to 13 This embodiment provides a reagent kit 20 , which includes a box body 100 and a microfluidic detection chip 600 .

[0106] The box body 100 includes an impedance detection cell (including a front cell 120 , a rear cell, and a pressure chamber 140 ) for performing impedance detection.

[0107] The microfluidic detection chip 600 is integrally connected to the box body 100, assembled connected or not connected and is located on the side of the impedance detection cell.

[0108] The microfluidic detection chip 600 is provided with a sample addition hole 601 for receiving the sample and then spreading it flatly inside the microfluidic detection chip 600 .

[0109] The box body 100 also includes a diluent pool 111, a hemolytic agent pool 106, an optical detection pool, and a staining and lysis pool 602. The diluent pool 111 is used to encapsulate the diluent, the hemolytic agent pool 106 is used to encapsulate the hemolytic agent, and the staining and lysis pool 602 is used to encapsulate the staining and lysis reagent. The optical detection pool is integrally connected or assembled with the box body 100.

[0110] The box body 100 also includes a mounting head accommodating pool and a sample accommodating pool. The mounting head accommodating pool is used to place the mounting head (tip head 201 or) puncture head 204, and the sample accommodating pool is used to place the sample tube.

[0111] Please also refer to Figure 13 This embodiment also provides a POCT blood cell analyzer, which includes a detection base 300, an image recognition base 610, and an image recognition device 620.

[0112] The detection base 300 is provided with a power supply for impedance detection (eg Figure 9 The conductive support 303 shown in the figure) is used to receive the aforementioned test kit 20, the image recognition base 610 is provided on the side of the test base 300, and the image recognition device 620 is provided above the image recognition base 610 for cooperating with the image recognition base 610 to perform image detection on the microfluidic detection sheet 600.

[0113] The POCT blood cell analyzer also includes a pipette 400, which is arranged above the detection seat 300. The POCT blood cell analyzer includes a metal shielding cover 500 that matches the detection seat 300. The metal shielding cover 500 can perform lifting or rotating movements. The image recognition device 620 is independently arranged or connected to the metal shielding cover 500.

[0114] The POCT blood cell analyzer and the reagent kit 20 provided in this embodiment can simultaneously support impedance detection and microfluidic image detection, optimize the efficiency of joint detection, and achieve better results.

[0115] Please refer to the fifth embodiment Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a frame 301, a detection seat 300, and a pipette 400.

[0116] The detection seat 300 is slidably or rotatably arranged relative to the frame 301, and the detection seat 300 reciprocates between the loading station and the sample detection station. When the detection seat 300 slides out or rotates out of the frame 301, it is located at the loading station to receive the reagent kit 20 or allow the reagent kit 20 to be taken out.

[0117] The pipette 400 is disposed in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent kit 20 .

[0118] The POCT blood cell analyzer also includes a liquid preparation station; the liquid preparation station is between the loading station and the sample detection station; or the liquid preparation station and the sample detection station are the same station.

[0119] The detection seat 300 is semi-enclosed with an upper opening. The detection seat 300 is snap-fitted with the reagent kit 20 . The detection seat 300 includes a main body 310 and a metal shielding seat ( 320 , 321 , 322 ) covering the outer periphery of the main body 310 .

[0120] The POCT blood cell analyzer further comprises a metal shielding cover 500 matched with the metal shielding seat (320, 321, 322). The metal shielding cover 500 is arranged above the detection seat 300 and is configured to be movable in the vertical direction at the sample detection station, and the vertical edge of the metal shielding cover 500 abuts against the upper surface of the metal shielding seat (320).

[0121] The POCT blood cell analyzer also includes a pressure building system. One end of the pressure building system is provided with an air pressure connection device 510 that extends into the metal shielding cover 500 and moves synchronously with the metal shielding cover 500. The reagent kit 20 is provided with a pressure application chamber 140 that is connected to the air pressure connection device 510.

[0122] This embodiment provides a method for using a POCT blood cell analyzer, the method comprising the following steps:

[0123] The detection seat 300 slides out or rotates out of the frame 301 to receive the reagent kit 20 at the loading station;

[0124] The detection seat 300 slides into or screws into the frame 301 and moves to the liquid preparation station;

[0125] The pipette 400 moves to load the pre-placed mounting head (tip head 201 or puncture head 204) on the reagent kit 20 and perform corresponding operations to complete the liquid preparation;

[0126] The detection seat 300 moves from the liquid preparation station to the sample detection station below the metal shielding cover 500;

[0127] The metal shielding cover 500 moves downward to cooperate with the detection base 300;

[0128] The air pressure connection device 510 connected to the metal shielding cover 500 starts to provide pressure to drain the liquid in the impedance detection pool on the reagent kit 20 for impedance detection.

[0129] The POCT blood cell analyzer and its use method provided in this embodiment have a high degree of automation, and the pre-treatment and liquid preparation operations of the test liquid are all automatically completed inside the instrument, avoiding the randomness and errors of manual operation.

[0130] Please refer to the sixth embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a detection seat 300 and a metal shielding cover 500.

[0131] The test base 300 reciprocates between the loading station and the sample testing station, receiving a reagent kit 20 equipped with an impedance testing cell at the loading station. A metal shielding cover 500 is located at the sample testing station, covering the test base 300 when the test base 300 moves to the sample testing station to shield electromagnetic signals. The POCT hematology analyzer also includes a liquid preparation station; this station may be located between the loading station and the sample testing station, or may be the same station as the sample testing station.

[0132] The detection seat 300 is in a semi-enclosed shape with an upper opening, and the metal shielding cover 500 is in a semi-enclosed shape with a lower opening. The detection seat 300 is snap-fitted with the reagent kit 20. The detection seat 300 includes a main body 310 and a metal shielding seat (320, 321, 322) covering the outer periphery of the main body 310. The detection seat 300 is configured to be able to translate or rotate under the metal shielding cover 500, and the metal shielding cover 500 is configured to be able to move up and down in the vertical direction.

[0133] The POCT blood cell analyzer also includes a pressure building system. One end of the pressure building system is provided with an air pressure connection device 510 that extends into the metal shielding cover 500 and moves synchronously with the metal shielding cover 500. The reagent kit 20 is provided with a pressure application chamber 140 that is connected to the air pressure connection device 510.

[0134] This embodiment also provides a method for using a POCT blood cell analyzer, the method comprising the following steps:

[0135] The detection seat 300 slides out or rotates out of the frame 301 to receive the reagent kit 20 at the loading station;

[0136] The detection seat 300 slides into or screws into the frame 301 and moves to the liquid preparation station;

[0137] The pipette 400 performs corresponding operations on the reagent kit 20 at the liquid preparation station;

[0138] The metal shielding cover 500 covers the detection base 300 and performs impedance detection. Specifically, the detection base 300 first moves to the bottom of the metal shielding cover 500, and then the metal shielding cover 500 moves downward to cover the detection base 300.

[0139] The pipette 400 prepares the reagent kit 20 by the liquid dispensing device, which includes: the pipette 400 draws the blood sample and reagents pre-installed in the reagent kit 20 and transfers them into the detection pool of the reagent kit 20 , wherein the reagents include a hemolytic agent and a diluent.

[0140] The POCT blood cell analyzer and its use method provided in this embodiment have a high degree of automation, and the pre-treatment and liquid preparation operations of the test liquid are all automatically completed inside the instrument, avoiding the randomness and errors of manual operation.

[0141] Please refer to the seventh embodiment. Figures 1 to 13 This embodiment provides a detection seat 300 , which includes a detection cavity 302 and a conductive support 303 .

[0142] The detection chamber 302 is used to receive the reagent kit 20 provided with an impedance detection cell. The reagent kit 20 is provided with electrodes, and the conductive support 303 is used to electrically connect the electrodes. The electrodes are conductive pillars 122 provided on opposite sides of the reagent kit 20.

[0143] like Figure 10 As shown, in one embodiment, the conductive support 303 includes a downwardly inclined spring portion 304, which is used to support and electrically connect the conductive pillar 122. The conductive support 303 also includes a fixed connection portion 305 integrally connected to the spring portion 304. The shape of the fixed connection portion 305 is not limited, and it can be provided with a fixing hole to facilitate fastening and assembly with screws.

[0144] like Figure 11 As shown, in another embodiment, the conductive support 303 includes an integrally connected, vertically disposed fixed connection portion 305 and a bent, extended portion 306 (which may be a V-shaped or arc-shaped bend). The bent portion 306 is displaced when the conductive post 122 is assembled from top to bottom. The bent portion 306 returns to its original position and abuts against the conductive post 122 when the conductive post 122 is fully assembled. This type of conductive support 303 has excellent elastic deformation capability, is durable, and can maintain a reliable electrical connection over a long period of time. The conductive support 303 can be a single member disposed on one side of the conductive post 122, or two members disposed on either side of the conductive post 122 to further enhance the reliability of the electrical connection. Specifically, the bent portion 306 may include a first bent portion 3061 and a second bent portion 3062, which are sequentially connected to the fixed connection portion 305. The first bent portion 3061 is displaced when the conductive post 122 is assembled from top to bottom, and the second bent portion 3062 abuts against the conductive post 122 when the conductive post 122 is fully assembled.

[0145] The detection seat 300 also includes a main body 310 and a metal shielding seat (320, 321, 322). The main body 310 is provided with a detection cavity 302. The side wall of the main body 310 is provided with an assembly groove 307 connected to the detection cavity 302. The conductive support 303 extends into the detection cavity 302 through the assembly groove 307. The metal shielding seat (320, 321, 322) is covered on the outer periphery of the main body 310 and is insulated from the conductive support 303. The metal shielding seat (320, 321, 322) includes side plates (320, 321) surrounding the main body 310 and a bottom plate (322) attached to the bottom of the main body 310.

[0146] The test kit 20 also includes an integrally connected or detachable optical detection pool. The detachable optical detection pool can be inserted through the rectangular insertion hole 107. The detection seat 300 also includes an optical detection component 330 corresponding to the optical detection pool. The optical detection component 330 includes a light-emitting component, a light-receiving component, and a detection cavity located between the light-emitting component and the light-receiving component. The angle between the axis of the light-emitting component and the axis of the light-receiving component ranges from 0 to 60 degrees, and can specifically be 0 degrees, 20 degrees, 30 degrees, 45 degrees, 60 degrees, etc.

[0147] The optical detection components 330 are divided into multiple groups, and the light source cavities of the multiple groups of optical detection components 330 are separated from each other by light-blocking walls 333 to avoid mutual interference of detection light.

[0148] For example, multiple groups of optical detection components 330 may include two groups of laser detection components 331 and one group of LED light source detection components 332. A light-blocking wall 333 is provided between the detection cavities of the laser detection components 331. The light-blocking wall 333 is preferably blackened to have better light absorption effect and poorer light reflection effect, so as to avoid reflected light affecting normal optical detection.

[0149] The detection base 300 also includes a Peltier 340, a radiator 350 and a fan 360, which are arranged in sequence near the detection cavity of the laser detection component 331. The radiator 350 includes a heat-absorbing substrate 351 attached to the Peltier 340 and heat-absorbing fins 352 vertically connected to the heat-absorbing substrate 351. The fan 360 can be arranged at the end of the heat-absorbing fins 352 away from the heat-absorbing substrate 351. By arranging the Peltier 340, the radiator 350 and the fan 360, the detection temperature can be controlled, and the detection results obtained by performing detection at the preset detection temperature are relatively more accurate.

[0150] A touch switch 334 is provided at the bottom of the detection chamber 302. The touch switch 334 can be used to detect the loading status of the reagent kit 20. When the reagent kit 20 is installed, the touch switch 334 is pressed. When the reagent kit 20 is not installed, the touch switch 334 is in a pop-up state.

[0151] This embodiment further provides a POCT blood cell analyzer, which includes a pipette 400 and the aforementioned detection seat 300 and the pipette 400 disposed above the detection seat 300 . The pipette 400 is used to perform corresponding operations on the reagent kit 20 .

[0152] The conductive support 303 of the POCT blood cell analyzer and its detection base 300 provided in this embodiment includes a downward-inclined spring portion 304, which has good adaptability and stability when electrically connected to the electrodes of the reagent kit 20, greatly reducing the possibility of poor contact.

[0153] An eighth embodiment provides a method for using a POCT blood cell analyzer, comprising the following steps:

[0154] In response to the reagent kit 20 being placed in the detection seat 300 , the pipette 400 is controlled to move to above the first mounting head accommodating pool of the reagent kit 20 and descend to load the first mounting head, wherein the first mounting head may be a tip head 201 ;

[0155] Control the pipette 400 to move to the diluent reservoir 111 of the reagent kit 20 to transfer the diluent into the forewell 120 (ie, the WBC detection reservoir and / or the RBC detection reservoir) for impedance detection;

[0156] Control the pipette 400 to move to the sample tube / sample dilution pool / other sample placement location to move the sample into the forepool 120 , wherein the sample in the sample dilution pool is the diluted sample;

[0157] Aerating or stirring the liquid to be tested in the forecell 120;

[0158] Control the metal shielding cover 500 to cover the detection base 300 to establish a communication relationship between the rear reservoir of the reagent kit 20 and the pressure building system;

[0159] Control the pressure building system to divert the test fluid from the front tank 120 into the rear tank;

[0160] The test liquid is detected by impedance detection method.

[0161] Among them, controlling the pipette 400 to move to above the first mounting head accommodating pool of the reagent kit 20 and descending to load the first mounting head includes: controlling the pipette 400 to move three-dimensionally relative to the first mounting head placement pool, so that the pipette 400 and the first mounting head are squeezed against each other to load the first mounting head.

[0162] Controlling the pipette 400 to move to the dilution pool 111 of the reagent kit 20 to move the dilution pool into the fore pool 120 for impedance detection and / or controlling the pipette 400 to move to the sample tube / sample dilution pool / other sample placement location to move the sample into the fore pool 120 includes: the pipette 400 makes three-dimensional movement relative to the fore pool 120 and uses the inner cavity of the first mounting head as a liquid transfer position to absorb the dilution liquid and / or sample. The volume of the inner cavity of the first mounting head is generally 100 microliters to 1000 microliters, and generally does not exceed 4 / 5 of the volume when in use to avoid liquid entering the pipeline of the pressure building system to cause contamination.

[0163] Mixing the test liquid in the fore cell 120 includes the pipette 400 sucking and discharging the test liquid through the first mounting head; or the pipette 400 injecting bubbles into the test liquid through the first mounting head; or the pipette 400 stirring the test liquid through the first mounting head.

[0164] After the test liquid in the fore cell 120 is mixed, the pipette 400 unloads the first mounting head to the first mounting head accommodating pool.

[0165] After the pipette 400 unloads the first mounting head into the first mounting head accommodating pool, the pipette 400 moves to above the second mounting head accommodating pool and descends to load the second mounting head.

[0166] After the pipette 400 moves to the second mounting head accommodating pool and descends to load the second mounting head, the following steps are included:

[0167] The pipette 400 moves to the sample tube / sample dilution pool / other sample placement location to move the sample into the light detection pool;

[0168] The test liquid is detected by optical detection method.

[0169] After the pipette 400 moves to the second mounting head accommodating pool and descends to load the second mounting head, the following steps are included:

[0170] The pipette 400 moves to the sample tube and transfers the sample into the staining and lysis pool 602 for incubation and then into the microfluidic detection chip 600;

[0171] The particles in the microfluidic detection chip 600 are detected by image detection method.

[0172] This embodiment further provides a POCT blood cell analyzer, which includes a detection base 300, a pipette 400, a pressure building system, a metal shielding cover 500, and a transmission assembly.

[0173] The detection seat 300 is used to receive the reagent kit 20, which is provided with a front pool 120 and a rear pool connected by micropores and is equipped with a first mounting head, diluent, and sample;

[0174] The pipette 400 is used to load the first mounting head and transfer the diluent and sample into the fore cell 120;

[0175] The metal shielding cover 500 is used to cover the detection seat 300, and the pressure building system is used to drain the liquid to be tested from the front tank 120 into the rear tank.

[0176] The transmission assembly is used to drive the detection base 300, the pipette 400 and the metal shielding cover 500 to perform one-dimensional, two-dimensional or three-dimensional movement. The transmission assembly can be composed of a motor, a screw rod, a nut, a slide rail, a slider, a gear, a rack, a synchronous belt, etc.

[0177] The reagent kit 20 is also equipped with a second mounting head and an optical detection cell. The pipette 400 is also used to load the second mounting head and move the sample into the optical detection cell for optical detection.

[0178] Alternatively, the test kit 20 is also equipped with a second mounting head, a staining and lysis reagent, and a microfluidic detection chip 600. The POCT blood cell analyzer also includes an image detection base 610 and an image detection device 620 that are spaced apart. The pipette 400 is also used to load the second mounting head and move the sample into the staining and lysis reagent for incubation, and then move the incubated sample into the microfluidic detection chip 600, and perform image detection on the microfluidic detection chip 600 through the image detection base 610 and the image detection device 620.

[0179] The POCT blood cell analyzer and its use method provided in this embodiment have a high degree of automation, complete automatic liquid preparation inside the instrument, have good process operation consistency, and can simultaneously support impedance detection, optical detection, and image detection.

[0180] Please refer to the ninth embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a detection base 300, a puncture head loading mechanism, a puncture head 204, and a pipette 400. The puncture head loading mechanism can be an independent mechanism or the same mechanism as the pipette 400.

[0181] The detection seat 300 is used to load the reagent kit 20. The reagent kit 20 is provided with a diluent pool and a hemolytic agent pool. The openings of the diluent pool and the hemolytic agent pool are provided with sealing films.

[0182] The puncture head 204 is used to puncture at least two sealing films on the reagent kit 20 loaded into the POCT blood cell analyzer one by one, and the puncture head loading mechanism is used to load the puncture head 204.

[0183] The puncture head 204 is disposed on the reagent box 20 and / or the detection base 300. The reagent box 20 and / or the detection base 300 are provided with a placement position matching the puncture head 204. After being loaded by the pipette 400, the puncture head 204 moves synchronously with the pipette 400.

[0184] After penetrating the sealing film, the puncture head 204 further swings horizontally, thereby enlarging the diameter of the through hole formed on the sealing film.

[0185] Preferably, at least two sealing films are located on the same straight line or the same arc, thereby making the trajectory of puncturing one by one relatively simple.

[0186] like Figure 7As shown, puncture tip 204 includes a main body 2042 and a sharp portion 2041. The sharp portion 2041 is located at one end of the main body 2042. The cross-section of the sharp portion 2041 can be in the shape of a straight line, a cross, a pound, or a Y. A flange 2044 is provided at the end of the main body 2042 away from the sharp portion 2041. The flange 2044 facilitates mounting of the puncture tip 204 in the puncture tip receiving well 104 and forms a stable abutment with the outer sleeve 402.

[0187] Please refer to the tenth embodiment Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a detection base 300 and a mounting head.

[0188] The test base 300 is used to load the test kit 20. The test kit 20 is provided with a diluent reservoir and a hemolytic agent reservoir. The openings of the diluent reservoir and the hemolytic agent reservoir are provided with sealing membranes. The mounting head is used to puncture the sealing membranes one by one on the test kit 20 loaded into the POCT blood cell analyzer. In this embodiment, the mounting head is a tip head 201 or a dedicated puncture head 204, which is provided on the test kit 20. The test kit 20 is provided with a placement position that matches the mounting head. The outer diameter of the puncture head 204 is larger than the outer diameter of the tip head 201. The opening formed by the puncture head 204 after passing through the sealing membrane is larger than the diameter of the opening where the tip head 201 extends. This prevents the formation of a negative pressure cavity in the reservoir when the tip head aspirates liquid, ensuring accuracy during aspiration.

[0189] The POCT blood cell analyzer further includes a pipette 400 , and the mounting head moves along with the pipette 400 after being loaded by the pipette 400 .

[0190] At least two sealing films are located on the same straight line or the same arc. This makes the trajectory of puncturing one by one relatively simple. After the mounting head passes through the sealing film, it further swings horizontally to expand the diameter of the through hole on the sealing film.

[0191] In one embodiment, the tip head 201 can first pierce the first through hole with a first stroke, and then pierce the second through hole with a second stroke to aspirate the sample. The first stroke is smaller than the second stroke, thereby avoiding the formation of a negative pressure sealing cavity during aspiration that affects the accuracy of aspiration.

[0192] In another embodiment, the tip is lifted after piercing the through hole and then the sample is aspirated, which can also avoid the formation of a negative pressure sealed cavity during aspiration and affecting the accuracy of aspiration.

[0193] The puncture head 204 includes a main body 2042 and a sharp portion 2041 disposed at an end of the main body 2042 .

[0194] The cross section of the sharp portion 2041 is in the shape of a straight line, a cross, a pound sign, or a Y. A flange portion 2044 is provided at one end of the main body 2042 away from the sharp portion 2041 .

[0195] In this embodiment, the tip head 201 can be directly used for puncture, or a dedicated puncture head 204 can be used for puncture.

[0196] Please refer to the eleventh embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a housing 10 , a pipette 400 , and a puncture head 204 .

[0197] The pipette 400 is disposed in the housing 10. The pipette 400 includes an airway tube 401. The puncture head 204 includes a main body 2042 and a sharp portion 2041. The sharp portion 2041 is disposed at one end of the main body 2042. The other end of the main body 2042 is provided with a receiving cavity 2045 for inserting the airway tube 401.

[0198] The sharp portion 2041 is used to puncture the sealing membrane of the reagent kit 20 during automated testing on a point-of-care (POCT) blood cell analyzer. The cross-section of the sharp portion 2041 is shaped like a straight line, a cross, a 'P', or a Y. The end of the main body 2042, distal from the sharp portion 2041, is provided with a flange 2044 to facilitate mounting on the body 100 of the reagent kit 20. The POCT blood cell analyzer also includes a test base 300 for mounting the reagent kit 20. The reagent kit 20 is provided with a puncture probe well, and the flange 2044 is supported on the outer periphery of the well.

[0199] The pipette also includes an outer sleeve 402 that fits over the airway tube 401. The lower end of the outer sleeve 402 abuts against the flange 2042. The end of the main body 2042, near the flange 2044, is provided with a plurality of ribs 2043 extending toward the sharp portion 2041 to enhance strength. The puncture tip 204 can be integrally molded from metal or plastic.

[0200] like Figure 12 As shown, this embodiment provides a reagent kit 20 , which includes a plurality of wells, one of which is used to place the aforementioned puncture head 204 .

[0201] The plurality of pools include a diluent pool 111 and / or a hemolytic agent pool 106 . The diluent pool 111 and / or the hemolytic agent pool 106 are provided with a sealing membrane (not shown).

[0202] The pool position also includes a WBC detection pool and / or an RBC detection pool for performing impedance detection.

[0203] like Figure 12As shown, the test kit 20 further includes a microfluidic detection chip 600 , which can perform image detection through an image detection base 610 and an image detection device 620 .

[0204] In this embodiment, the sealing film puncture operation can be performed without manual operation, and a dedicated puncture head 204 or tip head 201 can be used to achieve better consistency of operation.

[0205] Please refer to the twelfth embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a detection seat 300 and a pressure building component.

[0206] The detection seat 300 is used to install the test kit 20, which includes an impedance detection pool for impedance detection. The impedance detection pool includes a front pool 120 and a rear pool connected by micropores. The pressure building component is connected to the front pool 120 and / or the rear pool air path to provide pressure so that the test liquid in the front pool 120 flows to the rear pool through the micropores. It is also used to provide pressure to the front pool 120 or the rear pool before the test liquid is injected into the front pool 120 so that the micropores are unobstructed. When the pressure building component provides negative pressure, impedance detection can be performed. When the pressure building component provides positive pressure, the micropores can be backflushed to ensure the unobstructedness of the micropores.

[0207] The pressure-building assembly includes a syringe 220 and a pressure chamber 210 connected by an air path. The syringe 220 is used to establish positive or negative pressure in the pressure chamber 210. The pressure-building assembly is used to provide airflow to achieve bubble mixing in the test liquid or to clear the micropores. Specifically, air can be blown or sucked through the front cell 120 / back cell to clear the micropores.

[0208] The POCT blood cell analyzer includes multiple groups of gate valves, which connect the pressure chamber 210 and the syringe 220 .

[0209] The POCT blood cell analyzer includes a metal shielding cover 500 that cooperates with the detection base 300. The metal shielding cover 500 is provided with a pneumatic connection device 510 that communicates with the pressure chamber 210 or the syringe 220. The pneumatic connection device 510 is used to communicate with the rear pool or the front pool 120. The metal shielding cover 500 is configured to move in the vertical direction.

[0210] The POCT blood cell analyzer further includes a pipette 400 , which is disposed above the detection seat 300 and is used to perform corresponding operations on the reagent kit 20 .

[0211] The pipette 400 is connected to the air circuit of the pressure building component through a pipeline. The pipette 400 is used to load the mounting head on the reagent kit 20 and perform corresponding operations through the mounting head.

[0212] This embodiment also provides a method for using a POCT blood cell analyzer, which includes the following steps:

[0213] The receiving reagent kit is loaded into the detection seat 300, the reagent kit includes an impedance detection cell for performing impedance detection, and the impedance detection cell includes a front cell 120 and a rear cell connected by micropores;

[0214] Before the test liquid is injected into the front pool 120, pressure is provided to the front pool 120 or the rear pool to make the micropores unblocked. Specifically, this may include: providing pressure to the front pool 120 or the rear pool through the pressure in the pressure chamber 210 of the pressure building component to make the micropores unblocked; or providing pressure to the front pool 120 or the rear pool through the syringe 220 of the pressure building component to make the micropores unblocked.

[0215] Please refer to the thirteenth embodiment. Figures 1 to 13 This embodiment provides a point-of-care (POCT) blood cell analyzer, comprising a test base 300, a human-computer interaction module, and a processor. The test base 300 is used to receive a test kit 20. The test kit 20 is provided with at least two test pools for testing items. The test base 300 is provided with an auxiliary detector for testing items corresponding to the test pools. The human-computer interaction module is communicatively connected to the test base 300 and is used to select a test item. The human-computer interaction module is a combination of a touch screen or display 11 and mechanical buttons. The processor is signal-connected to the test base 300 and the human-computer interaction module and is used to receive detection signals from the test item and the auxiliary detector, and compare the detection items and detection signals to see if they correspond. If they do, subsequent operations can proceed normally. If they do not, the correct test kit 20 needs to be replaced or the correct test item needs to be reselected. The test pools are detachably attached to the test kit 20, and the auxiliary detector is used to detect whether a corresponding test pool exists.

[0216] The auxiliary detector includes an optical detection component 330, which includes a light-emitting component and a light-receiving component. The light signal obtained by the light-receiving component is used to assist in determining whether the item detection pool on the reagent kit 20 corresponds to the selected detection item.

[0217] The auxiliary detector includes a touch switch 334. The signal obtained by the touch switch 334 is used to assist in determining whether the item detection pool on the reagent kit 20 corresponds to the selected detection item.

[0218] The auxiliary detector includes an image recognition base 610 and an image recognition device 620 that are spaced apart from each other. The signals obtained by the image recognition base 610 and the image recognition device 620 are used to assist in determining whether the item detection pool on the reagent kit 20 corresponds to the selected detection item.

[0219] The POCT blood cell analyzer also includes a barcode scanner, which is used to obtain label information corresponding to the test kit 20. The label information may include name, type, production time and other information.

[0220] This embodiment also provides a method for using a POCT blood cell analyzer, comprising the following steps:

[0221] Select the items to be tested in the human-computer interaction module;

[0222] Obtaining the corresponding detection position information of the reagent kit 20;

[0223] The processor determines whether the detection position information corresponds to the selected detection item. If it does not correspond, a corresponding prompt is output. If it corresponds, subsequent operations can be performed normally. If it does not correspond, it is necessary to replace the correct reagent kit 20 or reselect the correct detection item.

[0224] In the step of obtaining the detection position information corresponding to the reagent kit 20, the detection position information corresponding to the reagent kit 20 includes a scattered light signal, a transmitted light signal or an electrical signal triggered by a micro switch.

[0225] Among them, the items to be tested include at least one of routine blood test, CRP test, SAA test, and blood cell classification test.

[0226] This embodiment also provides a method for using a POCT blood cell analyzer, including:

[0227] Acquiring detection information of items supported by the test kit 20 through the auxiliary detector;

[0228] Select the items to be tested in the human-computer interaction module;

[0229] It is determined whether the item detection information supported by the reagent kit 20 corresponds to the selected item to be detected. If not, a corresponding prompt is output.

[0230] The solution provided in this embodiment compares the items that the user wants to test with the items that the test kit can actually support before testing, which can better prevent mistakes.

[0231] Please refer to the fourteenth embodiment. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a housing 10 , a detection seat 300 , a pipette 400 , a first catheter 211 , and an anti-bending member 212 .

[0232] The detection seat 300 can be extended into or out of the housing 10 and is used to receive the reagent kit 20 provided with the impedance detection cell. The detection seat 300 is provided with a power supply (eg Figure 9 The conductive support 303 shown in FIG.

[0233] The pipette 400 is disposed in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent kit 20 .

[0234] One end of the first conduit 211 is connected to the air path of the pipette 400, and the other end of the first conduit 211 is connected to the pressure building system (eg Figure 5 The pressure-building system may include at least one of the following: syringe 220, pressure chamber 210, solenoid valves (SV1, SV2, SV3), a multi-way connector, and a manifold. The anti-bending member 212 is coupled to the first conduit 211 to prevent excessive bending of the first conduit 211, thereby blocking the internal air passage of the first conduit 211. The inner diameter of the first conduit 211 is relatively thin, typically ranging from 0.5 mm to 1.5 mm, and is prone to excessive bending and blockage in actual use.

[0235] The anti-bend member 212 can be a sleeve that fits around the outer periphery of the first conduit 211. The hardness of the sleeve is less than or equal to the hardness of the first conduit 211. Of course, the hardness of the sleeve can also be greater than the hardness of the first conduit 211. After the sleeve is fitted around the outer periphery of the first conduit 211, the overall hardness of the two is relatively increased. The first conduit 211 has a relatively greater hardness, and the deformation of the first conduit 211 itself is relatively small when the air pressure in the first conduit 211 changes, thereby improving the accuracy of sample aspiration. The wall thickness of the sleeve can be greater than or equal to the wall thickness of the first conduit 211, and the bending resistance of the sleeve can be less than or equal to the bending resistance of the first conduit 211. This can reduce wear of the sleeve on the first conduit 211 and prevent the first conduit 211 from excessive bending and blocking the internal air passage of the first conduit 211. The cross-section of the sleeve can be circular, or the cross-section of the sleeve can be C-shaped. The anti-bend member 212 can also be a spring that fits around the outer periphery of the first conduit 211. The anti-bending member 212 may also be a band wrapped around the outer circumference of the first tube 211 . The band may be a cloth band, a plastic band, or a metal band.

[0236] The ends of the first conduit 211 are respectively connected to the pipette 400 and the second syringe 223. A connector is provided at one end or both ends of the first conduit 211. The connector may be a clamp, a clamp joint, a flanged joint, a straight joint, or a threaded joint for airtight connection.

[0237] The POCT blood cell analyzer provided in this embodiment can effectively avoid the occurrence of air path obstruction and improve the stability of the instrument because the anti-bending member 212 is provided on the periphery of the relatively thin first catheter 211.

[0238] Please refer to the fifteenth embodiment. Figures 1 to 13This embodiment provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, a pipette 400, a pressure building system, a first catheter 211 and a second catheter 221.

[0239] The detection seat 300 can be extended into or out of the housing 10 and is used to receive the reagent kit 20 provided with the impedance detection cell. The detection seat 300 is provided with a power supply (eg Figure 9 The conductive support 303 shown in FIG.

[0240] The pipette 400 is disposed in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent kit 20 .

[0241] The two ends of the first conduit 211 are connected to the pipette 400 and the second syringe 223 respectively. The second conduit 221 connects the syringe 220, the pressure chamber 210, and the electromagnetic valves (SV1, SV2, SV3). Figure 5 The inner diameter of the T3 pipeline in the second conduit 221 is less than or equal to Figure 5 The inner diameter of the tubing (excluding T3 and T4 in the middle figure) is shown. The hardness of the first conduit 211 is greater than that of the second conduit 221. The first conduit 211 primarily controls the accuracy of sample aspiration or sample delivery, requiring minimal error from gas compression. Therefore, it has a greater hardness and a relatively smaller inner diameter. The second conduit 221, on the other hand, primarily builds pressure, requiring rapid speed and therefore allowing for a relatively larger inner diameter.

[0242] The ratio of the inner diameter of the first conduit 211 to the inner diameter of the second conduit 221 can be greater than 0.5 and less than or equal to 1. The ratio of the length of the first conduit 211 to the length of the second conduit 221 can be greater than 1 and less than or equal to 1.5. The relatively long first conduit 211 facilitates the installation of connectors (e.g., clamp connectors). In other words, the first conduit 211 is relatively thin and rigid, while the second conduit 221 is relatively thick and flexible.

[0243] like Figure 5 As shown, the POCT blood cell analyzer further includes an anti-bending member 212 , which is combined with the first tube 211 to prevent the first tube 211 from excessively bending and blocking the internal air guide channel of the first tube 211 .

[0244] The anti-bend member 212 can be a sleeve or spring that fits around the outer periphery of the first conduit 211. The sleeve's hardness is less than or equal to that of the first conduit 211. Alternatively, the sleeve's hardness can be greater than that of the first conduit 211. With the sleeve fitted around the outer periphery of the first conduit 211, the overall hardness of the two increases. The relatively greater hardness of the first conduit 211 leads to relatively less deformation of the first conduit 211 itself when the air pressure within the first conduit 211 changes, thereby improving the accuracy of suction and exhalation operations. The sleeve's wall thickness can be greater than or equal to that of the first conduit 211, and its bending resistance can be less than or equal to that of the first conduit 211. The sleeve's cross-section is circular or C-shaped. The anti-bend member 212 can also be a band wrapped around the outer periphery of the first conduit 211. The band can be made of fabric, plastic, or metal.

[0245] The hardness of the first conduit 211 is greater than or equal to that of the second conduit 221. The relatively greater hardness of the first conduit 211 results in relatively less deformation of the first conduit 211 itself when the air pressure within the first conduit 211 changes, thereby improving sample aspiration accuracy. The wall thickness of the first conduit 211 is less than or equal to that of the second conduit 221. The bending resistance of the first conduit 211 is less than or equal to that of the second conduit 221. This embodiment selects the inner diameter and hardness based on two different requirements, specifically meeting these requirements and achieving precise and efficient instrument operation. Furthermore, a bend prevention member 212 is designed for the thinner first conduit 211 to effectively prevent airway obstruction and further improve instrument stability.

[0246] Please refer to the sixteenth embodiment. Figures 1 to 14 This embodiment provides a method for using a POCT blood cell analyzer, which may include the following steps:

[0247] S10, receiving a power-on trigger signal, specifically, the user pressing a mechanical button to turn on the power;

[0248] S11, perform hardware self-test, that is, detect and feedback the voltage and current output of each port on the circuit board;

[0249] S12, determining whether the pipette is loaded with a mounting head, which can be determined by detecting elements such as a through-beam optical coupler or a reflective optical coupler, and the mounting head can be a tip head or a puncture head;

[0250] S18, if yes, the pipette unloads the mounting head, and the unloading location can be a specific location inside the instrument, or a certain pool position on the reagent cartridge 20 inside the instrument;

[0251] S13, if not, the whole machine is initialized, that is, each moving part is reset to zero or zero position is confirmed and the pressure is cleared. After the initialization is completed, the reagent kit 20 can be received and loaded, and fully automated sample pre-processing (automatic mixing of samples and corresponding reagents) is performed in the POCT blood cell analyzer. After the automated sample pre-processing is completed, the sample is tested.

[0252] Among them, after the pipette unloads the mounting head, it includes:

[0253] S19, the detection base 300 is removed from the chamber for the reagent kit 20 to be taken out. In this case, it is usually due to an accidental power outage during the last use, and the mounting head and the reagent kit 20 are still left inside the instrument;

[0254] S20, the detection seat 300 returns to the warehouse and performs the whole machine initialization step. This step is to ensure that there are no external components (installation head and reagent box 20) in the machine, and then the subsequent power-on process will not be affected by the presence of the tip head or reagent box 20.

[0255] The step of determining whether the pipette is loaded with an installation head also includes: determining whether a reagent kit 20 is installed in the detection base 300. Generally speaking, if there is an accidental power outage, both the reagent kit 20 and the installation head will be present inside the instrument at the same time. Therefore, it is possible to determine whether only the installation head is present inside the instrument, or to determine whether both the installation head and the reagent kit 20 are present inside the instrument at the same time. The determination of the presence of the reagent kit 20 can be made by means of a touch switch inside the detection base 300, or the like.

[0256] After the whole machine initialization step, it also includes:

[0257] S14, perform pressure detection to determine whether the pressure in the gas pipeline reaches the threshold range. The POCT blood cell analyzer provided by this application can be equipped with one, two or more pressure chambers 210, and establish positive or negative pressure through the syringe and reach a certain pressure value range. Specifically, after initialization, a pressure self-test process is performed. The pressure self-test requires the establishment of a certain value of negative pressure. Here, the pressure can be established between -25 and 30 kPa. The pressure establishment process relies on the solenoid valve, syringe, pressure sensor, pressure chamber 210 and gas pipeline. The pressure establishment process is as follows: During the pressure establishment process, the pressure sensor constantly detects the pressure of the pressure chamber 210. First, the syringe motor drives the syringe outward at the maximum speed, and at the same time opens the solenoid valve to establish a negative pressure operation. At this time, the syringe is directly connected to the pressure chamber 210. There is no other ventilation port open in the pressure chamber 210. When the syringe is pulled out, negative pressure can be established. If the syringe motor reaches its maximum stroke, that is, the syringe has been pulled to the maximum range, but the target pressure has not been built, the solenoid valve is closed. At this time, the syringe is initialized at the maximum speed and the maximum distance. After the initialization is completed, the solenoid valve is opened and the syringe is continued to be pulled outward to build pressure until the target pressure is reached. If the target pressure is not reached after the pressure building period, a pressure building failure is reported. At this time, the pressure building is completed. The pressure self-test continues. When the target pressure is built, if the pressure sensor detects normally, the solenoid valve is opened to connect the interior of the pressure chamber 210 to the air to release the negative pressure. After the pressure self-test, a simple blank test is performed. There is no need to place the test kit 20. The signal under no-load condition is directly measured to ensure that the test performance of the POCT blood cell analyzer is normal. Next, the main measurement process is carried out.

[0258] S15, perform signal detection, including judging the detection signal after the electromagnetic shielding component is matched and then judging the electromagnetic shielding effect and judging whether the current and voltage signals are within the threshold range, wherein the signal detection may include various photoelectric signals, etc., and also includes simulating the impedance method detection after the metal shielding cover and the metal shielding seat are combined and closed, and judging the shielding effect of the metal shielding cover and the metal shielding seat or the signal quality of the circuit board card itself according to the output results.

[0259] S16, enter the standby state, at this time you can choose to shut down or choose to perform testing.

[0260] Specifically, the step of entering the standby state includes:

[0261] S17, receiving a shutdown trigger signal, i.e., the user pressing the shutdown button. To prevent misoperation, it can be set so that after the user presses the shutdown button, the screen displays whether the shutdown is confirmed;

[0262] S21, determining whether the reagent kit 20 is present, which can be done by using a touch switch in the detection base 300;

[0263] S22, if the reagent kit 20 is determined to be in good condition, further determine the door status;

[0264] S24, if it is determined that the compartment door is open, a prompt is given to remove the reagent kit 20 and the process returns to the step of determining whether the reagent kit 20 is present;

[0265] S23, if it is determined that the door is closed, the door is opened and the reagent kit 20 is prompted to be removed, and then the process returns to the step of determining whether the reagent kit 20 is present;

[0266] S25, if it is determined that the reagent kit 20 is not present, further determining the door status;

[0267] S26, if it is determined that the door is open, close the door and prompt to turn off the power;

[0268] S27: If it is determined that the door is in the closed state, a prompt is given to turn off the power.

[0269] The steps to enter standby state include:

[0270] S31, receiving sample ID settings and measurement mode settings. Specifically, the screen may provide options such as mode settings, and provide some optional items for the user to select, such as CRP testing, SAA testing, blood testing, etc.;

[0271] S32, receiving a start test trigger signal, that is, a touch button or a physical button may be provided on the screen or on the instrument to generate a start test trigger signal;

[0272] S33, a pop-up window prompts to check whether the information to be tested (such as reagent information, or code scanning comparison / IC card reading comparison) matches the measurement mode. If not, click Cancel (S46) and return to step S31 to receive the sample ID setting and measurement mode setting;

[0273] S34, if there is a match, click confirm;

[0274] S35, automatically open the door;

[0275] S36, a pop-up window or other means may be used to prompt the user to insert the test kit;

[0276] S37, click OK after inserting the reagent kit;

[0277] S38, check whether the reagent kit 20 is present. If not, return to step S36 and prompt to put the reagent kit 20 in.

[0278] S39, if there is, the door is automatically closed;

[0279] S40, performing an automated liquid preparation test. The specific process of the automated liquid preparation test can refer to the aforementioned embodiment.

[0280] S41, displaying the test results, which may be specifically displayed on a display screen or output to a printing device;

[0281] S42, click OK;

[0282] S43, automatically opening the door and prompting to take out the reagent kit 20;

[0283] S44, the user takes out the reagent kit 20 and clicks OK;

[0284] S45, automatically close the door and enter standby mode.

[0285] The method of using the POCT blood cell analyzer provided in this embodiment is reasonable and orderly, and can quickly perform single item detection or multiple items detection at the same time.

[0286] This embodiment also provides a POCT blood cell analyzer, including a housing 10 , a detection seat 300 and a pipette 400 .

[0287] The detection seat 300 can extend into or out of the shell 10, and is used to receive the reagent kit 20 and reciprocate between the reagent kit 20 loading station and the sample detection station; the pipette 400 is arranged in the shell 10 and located above the detection seat 300, and is used to perform automated sample pre-processing on the reagent kit 20.

[0288] The test kit 20 includes a front cell 120 and a rear cell connected by micropores. The POCT blood cell analyzer also includes a pressure building system, which is arranged in the shell 10 and is used to make the test liquid flow from the front cell 120 to the rear cell for impedance detection.

[0289] The POCT blood cell analyzer also includes a metal shielding seat (320, 321, 322) for covering the detection seat 300, the test kit 20 includes a pressure application chamber 140 connected to the back pool, the metal shielding seat (320, 321, 322) is provided with an air pressure connection device 510 connected to the pressure building system, and when the metal shielding seat (320, 321, 322) covers the detection seat 300, the air pressure connection device 510 is sealed and docked with the pressure application chamber 140.

[0290] The pipette 400 is provided with a detection member (eg Figure 6 The optical coupler 405 shown is shown in the figure. The pipette 400 includes an air tube 401 and an outer sleeve 402 sleeved on the outer periphery of the air tube 401. The air tube 401 is used to load the mounting head installed on the reagent kit 20. When the air tube 401 is loaded with the mounting head, the air tube 401 is inserted into the mounting head so that the mounting head pushes the outer sleeve 402 to change its position along the axial direction of the air tube 401. The detection component is used to detect the position of the outer sleeve 402 and then determine whether the mounting head is sleeved on the air tube 401.

[0291] The pipette 400 further includes a head retraction mechanism (refer to the aforementioned blocking member 4031 ), which is used to stop the outer tube 402 from rising when the air guide tube 401 rises, so that the outer tube 402 can unload the mounting head.

[0292] The head-retracting mechanism is an electromagnet, a motor, or a U-shaped baffle arranged in the axial direction or radial direction relative to the air guide tube 401.

[0293] The pressure building system includes a pressure chamber 210, a first syringe 222 and a second syringe 223 that are linked together. The first syringe 222 is used to establish positive pressure or negative pressure for the pressure chamber 210. The second syringe 223 is connected to the pipette 400 and is used to use the inner cavity of the mounting head as a liquid transfer position to absorb reagents and / or samples when the pipette 400 is equipped with a mounting head.

[0294] The POCT blood cell analyzer includes a first catheter 211 and a second catheter 221. The first catheter 211 is connected between the second syringe 223 and the pipette 400. The second catheter 221 is used to connect the syringe and the pressure chamber 210. The inner diameter of the first catheter 211 is smaller than the inner diameter of the second catheter 221.

[0295] An anti-bending member 212 is provided on the outer periphery of the first conduit 211 . The anti-bending member 212 is a sleeve, a spring or a binding band.

[0296] This embodiment also provides a POCT blood cell analyzer, which includes a detection seat 300, a pipette 400 arranged above the detection seat 300, and a pressure building system. The detection seat 300 is used to receive and load the reagent kit 20. The reagent kit 20 is provided with a front pool 120 and a rear pool connected by micropores and is equipped with reagents, samples, and an installation head. The pressure building system includes a pressure chamber 210, an air pressure connection device 510, a first syringe 222, a second syringe 223, a driving member 224, a first solenoid valve SV1, a second solenoid valve SV2, and a third solenoid valve SV3.

[0297] The air pressure connection device 510 is connected to the pressure chamber 210, and is used to apply positive pressure or negative pressure to the front pool 120 or the rear pool to enable the liquid in the front pool 120 to flow to the rear pool; the first syringe 222 is connected to the pressure chamber 210 / pipette 400, and is used to establish positive pressure or negative pressure in the pressure chamber 210, and is also used to assist the pipette 400 in pipetting, or to bubble and mix the liquid in the front pool 120; the second syringe 223 is connected to the pipette 400 for pipetting; the driving member is used to simultaneously drive the first syringe 222 and the second syringe 223; the first solenoid valve SV1 selectively connects the first syringe 222 with the pressure chamber 210 and the second syringe 223; the second solenoid valve SV2 selectively connects the first solenoid valve with the pressure chamber 210 and the outside atmosphere; the third solenoid valve SV3 selectively connects the air pressure connection device with the pressure chamber 210.

[0298] The present application also provides a test kit 20, which includes a box body 100, a tip head accommodating pool (101, 102, 103), an installation head accommodating pool, a sample accommodating pool 105, a hemolytic agent pool 106, a rectangular insertion hole 107, a circular insertion hole 108, a front pool 120, an installation cavity, an assembly seat 160, a microporous sheet 170, a front pool 120 electrode 121, a rear pool electrode 165, a pressure application chamber 140, a tip head (201, 202, 203), a puncture head 204, a sample tube 205, a hemolytic agent container 206, a first pool body 207, a second pool body 208, etc.

[0299] The following will provide a detailed description based on various embodiments of the kit 20 .

[0300] Please refer to the seventeenth embodiment. Figure 15 and Figure 20 The embodiment of the present application provides a microporous sheet 170, which includes a sheet body 171. The sheet body 171 is provided with micropores 172 that allow cells to pass through one by one. The micropores 172 can be made into different specifications according to cells of different particle sizes. The sheet body 171 is a plastic sheet body or a ceramic sheet body. The mechanical strength of the plastic sheet body or the ceramic sheet body is relatively weak. The embodiment of the present application further provides a reinforcement part 173 on the sheet body 171 to ensure the mechanical strength of the microporous sheet 170, and at the same time has the effect of convenient installation. When the microporous sheet 170 is installed, the micropores 172 are not easily contaminated or worn by contact. The material cost of the plastic sheet body or the ceramic sheet body is relatively cheap, and it can be used as a disposable product without the need to use expensive materials that can be repeatedly cleaned.

[0301] The sheet 171 has a first surface 175 and a second surface 176 opposite to each other. The first surface 175 and / or the second surface 176 is provided with a reinforcement portion 173 .

[0302] The reinforcing portion 173 can be arranged close to the edge of the sheet body 171. The reinforcing portion 173 can be a structure such as a convex ring. The convex ring can be a continuous integral convex ring or a convex ring formed by multiple convex hulls. The outer edge of the convex ring can overlap or not overlap with the outer edge of the sheet body 171.

[0303] The convex ring is connected to the first surface 175 and / or the second surface 176 of the sheet body 171 via a vertical surface, an inclined surface or an arc surface, wherein the inclined surface or the arc surface can further reduce sample residue and improve detection accuracy.

[0304] The first surface 175 of the sheet 171 is provided with a concave drainage portion 174 around the micropore 172 . The concave drainage portion 174 may be spherical or conical.

[0305] The ratio of the thickness of the raised ring to the thickness of the sheet 171 is 0.2-2. Preferably, the ratio of the width of the raised ring to the radius of the sheet 171 is less than or equal to 1. If the thickness of the raised ring is too small, it cannot effectively strengthen the mechanical strength. If the thickness of the raised ring is too large, material is wasted. If the thickness of the raised ring is too large, the wall thickness of the micropores 172 of the microporous sheet 170 is difficult to control, resulting in a more complicated manufacturing process. The ratio of the width of the raised ring to the radius of the sheet 171 is 0.2-0.8. Similarly, if the width of the raised ring is too small, it cannot effectively strengthen the mechanical strength. If the width of the raised ring is too large, the wall thickness of the micropores 172 of the microporous sheet 170 is difficult to control, resulting in a more complicated manufacturing process. If the width of the raised ring is too large (e.g., close to the radius of the sheet 171), the axial channel of the micropore 172 becomes longer, further causing backflow of sample particles to be tested, affecting the accuracy of cell detection when passing through.

[0306] The microporous sheet 170 provided in this embodiment can improve the mechanical strength of the microporous sheet 170 by providing a reinforcing portion 173, thereby preventing the microporous sheet 170 made of plastic or ceramic material from being easily deformed during assembly. This embodiment also provides a reagent kit 20 including the aforementioned microporous sheet 170. The specific structure of the reagent kit 20 is referred to below.

[0307] Please refer to the eighteenth embodiment. Figure 15 and Figure 20 The embodiment of the present application provides a reagent kit 20 , which includes a box body 100 , an assembly seat 160 , and a microporous sheet 170 .

[0308] The box body 100 includes a forecell 120 , and the box body 100 is provided with forecell 120 electrodes 121 corresponding to the forecell 120 . The forecell 120 can be provided with two groups, one for cooperating with WBC (white blood cell) detection and the other for RBC (red blood cell) detection.

[0309] like Figure 18As shown, the assembly seat 160 is connected to the box body 100 , and the assembly seat 160 is provided with an axial drainage cavity 167 (the axial drainage cavity 167 may also be referred to as a back pool) and a back pool electrode 165 extending into the axial drainage cavity 167 .

[0310] like Figure 1 、 Figure 2 、 Figure 18 As shown, the microporous sheet 170 is provided with micropores 172 that allow cells to pass through one by one. The microporous sheet 170 is arranged between the front pool 120 and the axial drainage cavity 167. The front pool 120 and the axial drainage cavity 167 are connected through the micropores 172. The front pool 120 electrode 121 and the rear pool electrode 165 are respectively spaced apart and located on both sides of the microporous sheet 170.

[0311] The assembly seat 160 is detachably connected to the box body 100 .

[0312] In a specific embodiment, the forecell 120 electrode 121 can be integrally injection molded or detachably connected to the box body 100. The rear cell electrode 165 can be integrally injection molded or detachably connected to the assembly base 160. The forecell 120 electrode 121 and / or the rear cell electrode 165 are columnar electrodes, and the length of the rear cell electrode 165 is greater than or equal to the length of the forecell 120 electrode 121. The microporous sheet 170 can be integrally injection molded or detachably connected to the box body 100, or the microporous sheet 170 can be integrally injection molded or detachably connected to the assembly base 160.

[0313] The box body 100 is provided with a mounting cavity 130 , and the assembly seat 160 is snap-fitted, threadedly fitted, interference-fitted, laser-welded or adhesively fitted to the mounting cavity 130 .

[0314] The box body 100 and / or the assembly seat 160 are made of plastic. The front cell 120 electrode 121 is embedded in the box body 100 and is flush with, protruding from, or recessed in the outer surface of the box body 100. The rear cell electrode 165 is embedded in the assembly seat 160 and is flush with, protruding from, or recessed in the outer end surface of the assembly seat 160. The outer ends of the front cell 120 electrode 121 and the rear cell electrode 165 (i.e., the two ends away from each other) are used to connect to the working voltage, and the inner ends of the front cell 120 electrode 121 and the rear cell electrode 165 (i.e., the two ends close to each other) are in contact with the sample liquid to be tested. The axial drainage cavity 167 will be filled with the sample liquid to be tested during detection.

[0315] The reagent kit 20 further includes an inner sealing ring 164 , which is disposed between the microporous sheet 170 and the fore cell 120 , specifically between the first surface 175 of the microporous sheet 170 and the fore cell 120 , so that the sample liquid to be tested in the fore cell 120 can only enter the axial drainage cavity 167 through the micropores 172 .

[0316] The reagent box 20 further includes an outer sealing ring 166 , which is disposed between the assembly seat 160 and the free end of the mounting cavity 130 .

[0317] In one embodiment, the inner sealing ring 164 , the outer sealing ring 166 , the microporous sheet 170 and the assembly seat 160 are separate structural components.

[0318] In another embodiment, the inner sealing ring 164, the outer sealing ring 166, the microporous sheet 170 and the assembly seat 160 can be an integrated structural part to reduce the number of assembly parts, reduce the assembly difficulty, and save assembly time. Among them, the inner sealing ring 164 and the outer sealing ring 166 can be injection molded using a secondary injection molding process and a relatively soft plastic material.

[0319] In another embodiment, the inner sealing ring 164, the outer sealing ring 166, and the assembly base 160 are an integrated structural member, and the microporous sheet 170 is detachably connected to the integrated structural member, which can reduce the number of assembly parts while ensuring the manufacturing accuracy and yield rate of the microporous sheet 170. The inner sealing ring 164 has a certain degree of flexibility. When the microporous sheet 170 is installed with the integrated structural member, the inner sealing ring 164 can be installed behind the inner sealing ring 164 due to its flexibility, and the inner sealing ring 164 still seals the first surface 175 of the microporous sheet 170.

[0320] The reagent kit 20 provided in this embodiment has a novel structure and is easy to assemble.

[0321] Please refer to the nineteenth embodiment. Figure 15 and Figure 20 The embodiment of the present application provides an assembly seat 160 , which includes an assembly cylinder 168 and a rear cell electrode 165 .

[0322] The assembly cylinder 168 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611 (see FIG. Figure 16 ), the radial liquid outlet groove 1611 is used to discharge the gas or liquid in the axial drainage cavity 167; the rear cell electrode 165 is connected to the assembly tube 168 and extends into the axial drainage cavity 167.

[0323] The inner end of the radial liquid outlet groove 1611 and the inner end of the assembly tube 168 are separated by a first preset distance, and the inner end of the rear cell electrode 165 and the inner end of the assembly tube 168 are separated by a second preset distance, and the first preset distance is less than or equal to the second preset distance. When the first preset distance is less than the second preset distance, the bubbles in the axial drainage cavity 167 are more easily discharged from the axial drainage cavity 167. If bubbles remain in the axial drainage cavity 167, the detection accuracy will be affected. Wherein, the inner end is based on the interior of the box body 100 as a reference. When the front cell 120 is used as a reference, the end pointing to the front cell 120 is the inner end, and the end away from the front cell 120 is the outer end. For example, the inner end of the mounting cavity 130 is connected to the front cell 120 through the through hole 132, and the outer end of the mounting cavity 130 is an open end for receiving the assembly seat 160 for installation.

[0324] The axial length of the radial liquid outlet groove 1611 in the axial drainage cavity 167 is greater than or equal to the length of the rear cell electrode 165 extending into the axial drainage cavity 167. The inner end of the rear cell electrode 165 may or may not extend into the axial drainage cavity 167, that is, the inner end of the rear cell electrode 165 may protrude, be flush with, or be recessed from the bottom surface of the axial drainage cavity 167.

[0325] Furthermore, the outer surface of the assembly cylinder 168 is provided with a recessed area connected to the radial liquid outlet groove 1611 to form a guide groove 1612. The guide groove 1612 can allow the gas or liquid in the axial drainage chamber 167 to flow out through the radial liquid outlet groove 1611 directly above and then flow away through the guide groove 1612 obliquely above. In this way, the two assembly seats 160 can share a pressure chamber 140 connected to the two assembly seats 160. The pressure chamber 140 is connected to the through hole 133 (which can be Figure 17 The fan shape shown in FIG is connected to the installation cavity 130 and is further connected to the axial drainage cavity 167 through the guide groove 1612 and the radial liquid outlet groove 1611 in sequence.

[0326] The inner end of the assembly cylinder 168 is provided with a microporous sheet 170 that allows cells to pass through one by one. The specific structure of the microporous sheet 170 can refer to the above embodiment.

[0327] The distance from the inner end of the rear cell electrode 165 to the microporous sheet 170 is a third preset distance, which is 0.2-2 times the axial length of the axial drainage cavity 167. Experimental verification shows that within this range, better signal accuracy can be obtained when performing impedance detection.

[0328] A sink 1613 is provided at the inner end of the assembly cylinder 168 , and the microporous sheet 170 is cooperatively connected to the sink 1613 , and the reinforcement portion 173 of the microporous sheet 170 abuts against the sink 1613 .

[0329] like Figure 16As shown, the assembly cylinder 168 includes a rear pool cylinder 161, an end plate 162 and an outer cylinder 163 that are integrally connected. The rear pool cylinder 161 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611. The end plate 162 radially connects the rear pool cylinder 161 and the outer cylinder 163. The outer cylinder 163 is spaced apart and sleeved on the outer periphery of the rear pool cylinder 161.

[0330] The radial liquid outlet groove 1611 may be in the shape of a circular hole, a waist-shaped hole, or a rectangular hole.

[0331] The present application also provides a test kit 20, which includes a box body 100 and the aforementioned assembly seat 160, the box body 100 is provided with a front pool 120 and an installation cavity 130 that are connected to each other, the assembly cylinder 168 is connected to the installation cavity 130, and the front pool 120 is connected to the axial drainage cavity 167 through the micropores 172 of the microporous sheet 170.

[0332] The reagent kit 20 and its assembly seat 160 provided in this embodiment can facilitate the discharge of bubbles in the assembly cylinder 168 by limiting the distance between the inner end of the radial liquid outlet groove 1611 and the inner end of the rear cell electrode 165 and the inner end of the assembly cylinder 168, thereby avoiding bubbles remaining in the assembly cylinder 168 and affecting the detection accuracy during impedance detection.

[0333] Please refer to the 20th embodiment. Figure 15 and Figure 20 An embodiment of the present application provides an assembly seat 160 , which includes an assembly tube 168 and a rear cell electrode 165 . The rear cell electrode 165 is a columnar electrode, and the rear cell electrode 165 is connected to the assembly tube 168 .

[0334] The assembly cylinder 168 is provided with a radial liquid outlet groove 1611 and a snap-fitting portion 1631. The snap-fitting portion 1631 is aligned with the radial liquid outlet groove 1611. The snap-fitting portion 1631 can be a snap-fitting hole or a snap-fitting protrusion. Correspondingly, the outer surface of the mounting cavity 130 is provided with a snap-fitting block 131 corresponding to the snap-fitting hole. The opening directions of one of the snap-fitting portions 1631 and the radial liquid outlet groove 1611 are consistent and aligned, which makes it convenient to demold from the same direction during injection molding.

[0335] The assembly cylinder 168 is provided with an axial drainage cavity 167 , which is communicated with the radial liquid outlet groove 1611 , and the rear cell electrode 165 extends toward the axial drainage cavity 167 .

[0336] The rear cell electrode 165 can be embedded in the assembly seat 160 by injection molding and flush with, protruding from, or recessed into the outer end surface of the assembly seat 160 .

[0337] In the embodiment of the present application, the assembly cylinder 168 includes a rear pool cylinder 161, an end plate 162 and an outer cylinder 163 that are integrally connected. The rear pool cylinder 161 is provided with a radial liquid outlet groove 1611 and an axial drainage cavity 167, and the end plate 162 radially connects the rear pool cylinder 161 and the outer cylinder 163.

[0338] The outer cylinder 163 may extend axially to form a plurality of positioning protrusions 1632 that are spaced apart from each other, and the fastening portions 1631 are disposed corresponding to the positioning protrusions 1632 .

[0339] There is a first preset distance between the inner end of the radial liquid outlet groove 1611 and the inner end of the assembly cylinder 168, and a second preset distance between the inner end of the rear cell electrode 165 and the inner end of the assembly cylinder 168. The first preset distance is less than or equal to the second preset distance.

[0340] The rear cell electrode 165 and the assembly tube 168 may be integrally formed or detachably connected.

[0341] A sink 1613 is provided at the inner end of the rear cell tube 161 for assembling a microporous sheet 170 that allows cells to pass through one by one.

[0342] The present application also provides a test kit 20, which includes a box body 100 and the aforementioned assembly seat 160, the box body 100 is provided with a front pool 120 and an installation cavity 130 that are connected to each other, the assembly cylinder 168 of the assembly seat 160 is connected to the installation cavity 130, and the front pool 120 is connected to the axial drainage cavity 167 through the micropores 172 of the microporous sheet 170.

[0343] The reagent kit 20 and its assembly seat 160 provided in this embodiment can facilitate the demolding operation after mold forming by aligning the radial liquid outlet groove 1611 and the fastening portion 1631.

[0344] Please refer to the twenty-first embodiment. Figure 15 and Figure 20 An embodiment of the present application provides an assembly seat 160 , which includes an assembly cylinder 168 and a rear cell electrode 165 .

[0345] The assembly cylinder 168 is provided with an axial drainage chamber 167 and a radial liquid outlet groove 1611 that are connected to each other. The outer surface of the assembly cylinder 168 is provided with a recessed area connected to the radial liquid outlet groove 1611 to form a guide groove 1612. The guide groove 1612 can make the gas or liquid in the axial drainage chamber 167 flow out through the radial liquid outlet groove 1611 (for example, directly above) and then flow smoothly to the pressure application chamber 140 through the guide groove 1612 (for example, obliquely above). Thus, the two assembly seats 160 can share a pressure application chamber 140 connected to the two assembly seats 160. The pressure application chamber 140 is connected to the pressure application chamber 140 through the through hole 133 (which can be Figure 17The fan shape shown in FIG is connected to the installation cavity 130 and is further connected to the axial drainage cavity 167 through the guide groove 1612 and the radial liquid outlet groove 1611 in sequence.

[0346] The rear cell electrode 165 is connected to the assembly tube 168 and extends to the axial drainage cavity 167.

[0347] Guide groove 1612 is circumferentially disposed along the outer surface of assembly tube 168. This arc-shaped structure, with a central angle ranging from 0° to 360°, allows the sample liquid to flow more smoothly toward pressure chamber 140. The depth of guide groove 1612 is 1 / 5 to 4 / 5 the wall thickness of assembly tube 168, ensuring smooth liquid flow while maintaining mechanical strength and resisting damage.

[0348] The radial liquid outlet groove 1611 is connected to the guide groove 1612 via a transition surface, and the transition surface is a vertical plane, an inclined surface or an arc surface.

[0349] The rear cell electrode 165 is embedded in the assembly seat 160 and is flush with, protruding from, or recessed into the outer end surface of the assembly seat 160 .

[0350] There is a first preset distance between the inner end of the radial liquid outlet groove 1611 and the inner end of the assembly cylinder 168; there is a second preset distance between the inner end of the rear cell electrode 165 and the inner end of the assembly cylinder 168; the first preset distance is less than or equal to the second preset distance.

[0351] like Figure 16 As shown in the figure, the assembly cylinder 168 includes a rear pool cylinder 161, an end plate 162 and an outer cylinder 163 connected as one piece. The rear pool cylinder 161 is provided with a radial liquid outlet groove 1611 and an axial drainage cavity 167. The end plate 162 radially connects the rear pool cylinder 161 and the outer cylinder 163.

[0352] The outer cylinder 163 extends axially to form a plurality of positioning protrusions 1632 spaced apart from each other, and the fastening portions 1631 are disposed corresponding to the positioning protrusions 1632 .

[0353] The present application also provides a test kit 20, which includes a box body 100 and the aforementioned assembly seat 160, the box body 100 is provided with a front pool 120 and an installation cavity 130 that are connected to each other; the assembly cylinder 168 of the assembly seat 160 is connected to the installation cavity 130, and the front pool 120 is connected to the axial drainage cavity 167 through the micropores 172 of the microporous sheet 170.

[0354] The test kit 20 and its assembly seat 160 provided in this embodiment can facilitate two assembly seats 160 to share a pressure action chamber 140 connected to the two assembly seats 160 by arranging a radial liquid outlet groove 1611 on the assembly cylinder 168 and forming a recessed area on the outer surface of the radial liquid outlet groove 1611 to form a guide groove 1612, thereby facilitating negative pressure drainage of the pressure action chamber 140.

[0355] Please refer to the twenty-second embodiment. Figure 15 and Figure 20 The embodiment of the present application provides an assembly seat 160 , which includes an assembly cylinder 168 and a rear cell electrode 165 .

[0356] The assembly tube 168 is a plastic assembly tube having an axial drainage cavity 167 and a radial liquid outlet groove 1611 connected to each other. The radial liquid outlet groove 1611 is used to discharge the gas or liquid in the axial drainage cavity 167 . The rear cell electrode 165 is connected to the assembly tube 168 and extends into the axial drainage cavity 167 .

[0357] The outer surface of the assembly cylinder 168 is provided with a recessed area connected to the radial liquid outlet groove 1611 to form a guide groove 1612. The guide groove 1612 can make the gas or liquid in the axial drainage chamber 167 flow out through the radial liquid outlet groove 1611 (for example, directly above) and then flow smoothly to the pressure application chamber 140 through the guide groove 1612 (for example, obliquely above). Thus, the two assembly seats 160 can share a pressure application chamber 140 connected to the two assembly seats 160. The pressure application chamber 140 is connected to the pressure application chamber 140 through the through hole 133 (which can be Figure 17 The fan shape shown in FIG is connected to the installation cavity 130 and is further connected to the axial drainage cavity 167 through the guide groove 1612 and the radial liquid outlet groove 1611 in sequence.

[0358] The assembly cylinder 168 is provided with a snap-fitting portion 1631, which is aligned with the radial liquid outlet groove 1611. The snap-fitting portion 1631 can be a snap-fitting hole. The alignment of the snap-fitting portion 1631 and the radial liquid outlet groove 1611 can facilitate demolding from the same direction during injection molding.

[0359] A first predetermined distance exists between the inner end of radial liquid outlet groove 1611 and the inner end of assembly tube 168; a second predetermined distance exists between the inner end of rear cell electrode 165 and the inner end of assembly tube 168. The first predetermined distance is less than or equal to the second predetermined distance. When the first predetermined distance is less than the second predetermined distance, bubbles within axial drainage cavity 167 are more easily discharged. If bubbles remain within axial drainage cavity 167, detection accuracy may be affected.

[0360] The axial length of the radial liquid outlet groove 1611 in the axial drainage cavity 167 is greater than or equal to the length of the rear cell electrode 165 extending toward the axial drainage cavity 167 .

[0361] The inner end of the assembly tube 168 is provided with a microporous sheet 170 that allows cells to pass through one by one. The distance between the inner end of the back cell electrode 165 and the microporous sheet 170 is a third predetermined distance, which is 0.2-2 times the axial length of the axial drainage lumen 167. The inner end of the assembly tube 168 is provided with a sink 1613, and the microporous sheet 170 is matingly connected to the sink 1613.

[0362] In the embodiment of the present application, the assembly cylinder 168 includes a rear pool cylinder 161, an end plate 162 and an outer cylinder 163 that are integrally connected. The rear pool cylinder 161 is provided with an axial drainage chamber 167 and a radial liquid outlet groove 1611. The end plate 162 radially connects the rear pool cylinder 161 and the outer cylinder 163. The outer cylinder 163 is spaced apart and sleeved on the outer periphery of the rear pool cylinder 161. The buckling portion 1631 is provided on the outer cylinder 163.

[0363] The present application also provides a test kit 20, which includes a box body 100 and the aforementioned assembly seat 160, the box body 100 is provided with a connected front pool 120 and an installation cavity 130; the assembly cylinder 168 of the assembly seat 160 is connected to the installation cavity 130, the front pool 120 is connected to the axial drainage cavity 167 through the micropore 172 of the microporous sheet 170, the front pool 120, the installation cavity 130, the axial drainage cavity 167, the radial liquid outlet groove 1611, and the pressure action cavity 140 together constitute a drainage channel, and the axial drainage cavity 167 can receive negative pressure to draw the sample to be tested in the front pool 120 through the micropore 172 of the microporous sheet 170.

[0364] The reagent kit 20 and its assembly base 160 provided in this embodiment have novel structures and are easy to manufacture and assemble.

[0365] Please refer to the twenty-third embodiment. Figure 15 and Figure 20 The embodiment of the present application provides a reagent kit 20 , which includes a box body 100 , a hemolytic agent pool 106 disposed on the box body 100 , and a diluent pool 111 disposed near the hemolytic agent pool 106 .

[0366] At least any two of the hemolytic agent reservoir 106, the diluent reservoir 111, and the cartridge body 100 are integrally connected, and at least any two of the hemolytic agent reservoir 106, the diluent reservoir 111, and the cartridge body 100 are detachably connected. In other words, the cartridge body 100, the hemolytic agent reservoir 106, and the diluent reservoir 111 can be a conjoined structure or any separate structure.

[0367] For example, the box body 100, the hemolytic agent pool 106, and the diluent pool 111 are three independent parts and can be detachably connected to each other; or, the diluent pool 111 and the box body 100 are an integral structural part, and a separate hemolytic agent container 206 is detachably assembled to the hemolytic agent pool 106; or, the hemolytic agent pool 106 and the box body 100 are an integral structural part, and a separate diluent pool test tube is detachably assembled to the diluent pool 111; or, the hemolytic agent pool 106 and the diluent pool 111 are connected as a whole and are detachably connected to the box body 100.

[0368] Any two of the hemolytic agent pool 106, the diluent pool 111, and the box body 100 form an assembly, and an assembly portion (e.g., the inner peripheral wall of the insertion hole provided on the box body 100) is provided on the assembly, and a disassembly portion (e.g., the inner peripheral wall of the insertion hole provided on the box body 100) is provided on the other one of the hemolytic agent pool 106, the diluent pool 111, and the box body 100. Figure 15 The outer peripheral wall of the hemolytic agent container 206 shown in FIG), the disassembly portion is cooperatively connected with the assembly portion.

[0369] The surface of the assembly portion or the disassembly portion is further provided with a protruding structure 2061 , which enables the hemolytic agent container 206 to abut and fit tightly with the insertion hole provided on the box body 100 .

[0370] In one embodiment, the box body 100 is provided with two assembly positions, and the hemolytic agent pool 106 and the diluent pool 111 are respectively installed in the two assembly positions.

[0371] In the embodiment of the present application, the box body 100 includes a front pool 120 and an installation cavity 130 that are connected to each other. The front pool 120 and the installation cavity 130 are connected by a through hole 132 (see Figure 17 and Figure 18 ) are connected, the front pool 120 is equipped with a front pool 120 electrode 121, the reagent kit 20 also includes an assembly seat 160 connected to the installation cavity 130, the assembly seat 160 is provided with a microporous sheet 170 and a rear pool electrode 165, the microporous sheet 170 is installed at the through hole 132, the front pool 120 electrode 121 and the rear pool electrode 165 are respectively spaced apart and located on both sides of the microporous sheet 170, the assembly seat 160 includes an assembly cylinder 168, the assembly cylinder 168 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611.

[0372] In the embodiment of the present application, the assembly cylinder 168 includes a rear pool cylinder 161, an end plate 162 and an outer cylinder 163 that are integrally connected. The rear pool cylinder 161 is provided with an axial drainage chamber 167 and a radial liquid outlet groove 1611. The end plate 162 radially connects the rear pool cylinder 161 and the outer cylinder 163. The outer cylinder 163 is spaced apart and sleeved on the outer periphery of the rear pool cylinder 161.

[0373] The usage of the diluent pool 111 is generally relatively large, so in the embodiment of the present application, the volume of the diluent pool 111 is greater than or equal to the volume of the hemolytic agent pool 106 .

[0374] The open ends of the diluent reservoir 111 and the hemolytic agent reservoir 106 are provided with sealing films to facilitate long-term storage of the diluent and hemolytic agent.

[0375] The test kit 20 provided in this embodiment has a novel structure and multiple detachable assembly and disassembly schemes, and can adapt to various different detection requirements.

[0376] Please refer to the twenty-fourth embodiment. Figure 15 and Figure 20 , an embodiment of the present application provides a reagent kit 20, the reagent kit 20 includes a box body 100 and at least one item detection pool, and the at least one item detection pool is integrally connected to the box body 100 or detachably connected.

[0377] The item detection pool includes a first item detection pool for performing a first item detection and a second item detection pool for performing a second item detection. The first item detection pool and / or the second item detection pool are integrally connected to the box body 100 or are detachably connected.

[0378] The test kit 20 further includes a third item detection pool for performing a third item detection. The third item detection pool is integrally connected to the box body 100 or is detachably connected thereto.

[0379] The second item detection pool and the third item detection pool are arranged on the same side of the first item detection pool; or the second item detection pool and the third item detection pool are respectively arranged on both sides of the first item detection pool.

[0380] The detection items of the first item detection pool, the second item detection pool, and the third item detection pool can be selected from specific protein detection, biochemical detection, immunological detection, and routine blood test. For example, routine blood test can be performed by cooperating with the front pool 120, the installation cavity 130, the assembly seat 160, and the pressure action cavity 140, and specific protein detection, biochemical detection, immunological detection, etc. can be performed by cooperating with the optical detection cup.

[0381] The detection items of the first item detection pool, the second item detection pool, and the third item detection pool are the same, different, or not completely the same; or any one of the first item detection pool, the second item detection pool, and the third item detection pool supports more than two detection items.

[0382] The box body 100 is provided with an insertion hole, which may include a rectangular insertion hole 107 and a circular insertion hole 108. The second item detection pool is inserted through the insertion hole, or the second item detection pool is provided with a flange to cooperate with the insertion hole.

[0383] The second item detection cell includes a first cell body 207, a second cell body 208 and a connector 2071 connecting the first cell body 207 and the second cell body 208. The connector 2071 connects the first cell body 207 and the second cell body 208 to the box body 100 in the form of an assembly.

[0384] The first item detection pool and / or the second item detection pool are transparent plastic pools or glass pools. The opening ends of the first item detection pool and / or the second item detection pool are provided with sealing membranes, which can be punctured by the puncture head 204 provided on the box body 100 when needed.

[0385] The test kit 20 provided in this embodiment has a novel structure and multiple detachable assembly and disassembly schemes, and can adapt to various different detection requirements.

[0386] Please refer to the twenty-fifth embodiment. Figure 15 and Figure 20 The embodiment of the present application provides a test kit 20, which includes a box body 100. The box body 100 includes a front pool 120, an installation cavity 130 and a pressure application chamber 140. The front pool 120 is connected to the installation cavity 130, and the pressure application chamber 140 is connected to the installation cavity 130. The pressure application chamber 140 is vertically arranged and the installation cavity 130 is horizontally arranged.

[0387] The opening end of the installation cavity 130 faces the outer surface of the box body 100 . The reagent kit 20 further includes an assembly seat 160 , which is connected to the installation cavity 130 . The assembly seat 160 is provided with an axial drainage cavity 167 , which is connected to the pressure application cavity 140 .

[0388] A drainage gap is formed between the installation cavity 130 and the assembly seat 160 , and the axial drainage cavity 167 is connected to the pressure application cavity 140 through the drainage gap.

[0389] A radial liquid outlet groove 1611 is provided on the assembly seat 160, and the axial drainage chamber 167 is connected to the pressure application chamber 140 through the radial liquid outlet groove 1611; the assembly seat 160 is provided with a guide groove 1612 adjacent to the radial liquid outlet groove 1611, and the axial drainage chamber 167 is connected to the pressure application chamber 140 through the radial liquid outlet groove 1611 and the guide groove 1612.

[0390] The assembly seat 160 is provided with a microporous sheet 170 and a rear cell electrode 165 , and the fore cell 120 is provided with a fore cell 120 electrode 121 . The fore cell 120 electrode 121 and the rear cell electrode 165 are respectively spaced apart and located on both sides of the microporous sheet 170 .

[0391] The assembly seat 160 and the installation cavity 130 are snap-fitted, threaded, interference-fitted, laser-welded, or adhesively bonded.

[0392] The reagent kit 20 further includes an inner sealing ring 164 and an outer sealing ring 166 . The inner sealing ring 164 is disposed between the microporous sheet 170 and the box body 100 , and the outer sealing ring 166 is disposed between the assembly seat 160 and the box body 100 .

[0393] In the embodiment of the present application, the front pool 120 and the installation cavity 130 are both in two groups, and one pressure action cavity 140 is connected to the two groups of installation cavities 130 .

[0394] The test kit 20 provided in this embodiment has a novel structure. Its front pool 120, installation cavity 130, and pressure action cavity 140 are interconnected and arranged vertically in sequence. The open end of the pressure action cavity 140 is located on the upper surface of the box body 100. The negative pressure source can be applied from above the box body 100, thereby simplifying the structure of the box body 100 and avoiding the side of the box body 100 having many protruding structures.

[0395] Please refer to the twenty-sixth embodiment. Figure 15 and Figure 20 An embodiment of the present application provides a reagent kit 20, which includes a plurality of first pools, and the centers of the plurality of first pools are arranged roughly in a first straight line or a first arc.

[0396] The reagent kit 20 further includes a plurality of second wells arranged approximately in a second straight line at the center, and the first straight line and the second straight line are arranged in parallel or vertically spaced relation.

[0397] Alternatively, the reagent kit 20 includes a plurality of second pool positions arranged in a second arc at the center, the first arc and the second arc are arranged in parallel and spaced apart, and the arc can be an arc corresponding to a central angle of 0 to 360.

[0398] The plurality of first cell locations and / or the plurality of second cell locations include impedance detection cells and / or optical detection cells.

[0399] The multiple first pool positions and / or the multiple second pool positions also include a diluent pool 111, which is used to encapsulate the diluent; the multiple first pool positions and / or the multiple second pool positions also include a hemolytic agent pool 106, which is used to encapsulate the hemolytic agent; or the multiple first pool positions and / or the multiple second pool positions also include a sample dilution pool 112, which is used for sample dilution.

[0400] The impedance detection pool is provided with a light-transmitting detection window for cooperating with optical detection, that is, one of the front pools 120 is provided with a light-transmitting detection window for cooperating with optical detection. The front pool 120 can be made of transparent plastic as a whole, and the transmittance and smoothness of the light-transmitting detection window can be the same as or higher than other parts of the front pool 120.

[0401] The plurality of first pool positions and / or the plurality of second pool positions further include a plurality of insertion holes.

[0402] The plurality of first pool positions and / or the plurality of second pool positions include at least one tip head accommodating pool (101, 102, 103) and / or a sample accommodating pool 105.

[0403] The plurality of first pool positions and / or the plurality of second pool positions include at least one puncture head accommodating pool 104. The test kit 20 provided in this application is a biodegradable plastic test kit 20. By arranging the plurality of pool positions in a straight line, it is possible to facilitate the movement of a pipetting device over a shorter path during automated testing, wherein the pipetting device is used to transfer and mix the liquids in each pool.

[0404] Please refer to the twenty-seventh embodiment. Figure 15 and Figure 20 The embodiment of the present application provides a test kit 20, which includes a box body 100. The box body 100 is provided with a detection area (such as the area composed of a front pool 120, an installation cavity 130, a pressure application chamber 140, and an assembly seat 160), a reagent area (such as the area composed of a diluent pool 111, a hemolytic agent pool 106, and a sample holding pool 105), and an accessory placement area (such as the area composed of tip head holding pools 101, 102, 103 and a puncture head holding pool 104).

[0405] The box body 100 also includes an expandable area (such as the area formed by the rectangular insertion hole 107 and the circular insertion hole 108 in the figure), which is arranged close to the detection area, the expandable area is located on one side of the detection area, or the expandable area is located on one side of the reagent area.

[0406] The accessory placement area is set close to the reagent area, and the detection area is set close to the reagent area.

[0407] The detection area includes an impedance detection cell and / or an optical detection cell, and the optical detection cell is used to detect any one of the parameters of HGB, CRP, and SAA.

[0408] The reagent area includes at least one of a diluent reservoir, a hemolytic agent reservoir, and a sample holding reservoir 105 .

[0409] The reagent area includes a cell body area, which is used to detachably mount at least one of a diluent container, a hemolytic agent container 206 and a sample holding cell 105 .

[0410] The accessory placement area includes placement positions for tip heads 201 , 202 , 203 and / or puncture head 204 .

[0411] The expandable area includes a detachable optical detection cell and / or an impedance detection cell. The optical detection cell is used to detect any one of the parameters of HGB, CRP, and SAA.

[0412] The test kit 20 provided in the present application is a degradable plastic test kit 20 , which can facilitate the movement of the pipetting device over a shorter path during automatic detection through a partitioned setting.

[0413] Please refer to the twenty-eighth embodiment. Figure 15 and Figure 20 The embodiment of the present application provides a reagent kit 20, which includes a box body 100. The box body 100 is provided with a detection area (such as the area composed of a front pool 120, an installation cavity 130, a pressure application chamber 140, and an assembly seat 160), a reagent area (such as the area composed of a diluent pool 111, a hemolytic agent pool 106, and a sample holding pool 105), and an accessory placement area (such as the area composed of tip head holding pools 101, 102, 103 and a puncture head holding pool 104). The reagent area is arranged near the accessory placement area.

[0414] The box body 100 also includes an expandable area (such as the area formed by the rectangular insertion hole 107 and the circular insertion hole 108 in the figure), which is arranged near the detection area. Alternatively, the expandable area is arranged near the side of the detection area or near the side of the reagent area.

[0415] The reagent area includes a plurality of reagent cells, and the plurality of reagent cells are arranged roughly in a straight line or an arc.

[0416] The detection area includes an impedance detection cell and / or an optical detection cell, and the optical detection cell is used to detect any one of the parameters of HGB, CRP, and SAA.

[0417] The accessory placement area includes placement positions for tip heads 201 , 202 , 203 and / or puncture head 204 .

[0418] The expandable area includes a detachable optical detection cell and / or an impedance detection cell. The optical detection cell is used to detect any one of the parameters of HGB, CRP, and SAA.

[0419] The reagent area includes at least two pool body areas, and the at least two pool body areas include bottomed pools and / or bottomless insertion holes.

[0420] The accessory area includes one or more bottomed pools for mounting tip heads 201 , 202 , 203 and / or piercing head 204 .

[0421] The expandable area is provided with a circular insertion hole 108 and a rectangular insertion hole 107. The circular insertion hole 108 and the rectangular insertion hole 107 are used to install a detection cell, which can be an optical detection cell and / or an impedance detection cell. The test kit 20 provided in this application is a biodegradable plastic test kit 20. The partitioned arrangement facilitates the movement of the pipette device over a shorter path during automated testing.

[0422] Please refer to the twenty-ninth embodiment. Figure 15 and Figure 20 An embodiment of the present application provides a reagent kit 20, which includes a box body 100. The reagent kit 20 is used for sample detection. The box body 100 is provided with a supporting portion so that the reagent kit 20 can be stably supported on a receiving surface.

[0423] In one embodiment, the box body 100 includes two support plates (not shown), which serve as support portions. The support plates may be provided with labels such as bar codes, QR codes, or identification chips to record relevant parameters of the reagent kit 20.

[0424] At least one supporting platform or supporting surface is provided at the box body 100 near the center of gravity of the reagent box 20 , and the supporting platform or supporting surface serves as a supporting portion.

[0425] In one embodiment, the box body 100 is provided with three supporting protrusions, which form a triangle, and the projection of the center of gravity of the reagent box 20 falls within the area surrounded by the triangle.

[0426] In one embodiment, the box body 100 includes at least one pool body, and the bottom of the pool body can serve as a support portion.

[0427] Optionally, the supporting plate or the supporting platform or the supporting surface or the supporting protrusion and the bottom of the pool body serve together as the supporting portion.

[0428] The tank body includes a receiving chamber and a skirt 124 extending downward from the receiving chamber (refer to Figure 18 , structure below the bottom 123 of the front pool 120), the skirt 124 serves as a support portion, and the accommodating cavity can be used to hold reagents, samples, or accessories (201-204).

[0429] The box body 100 provided in this application is a degradable plastic box body. The reagent box 20 is provided with an impedance detection cell and / or an optical detection cell.

[0430] The reagent kit 20 provided in this embodiment is provided with more pool bodies, and a stable support may be formed through the bottom of the pool body and / or an additional support portion to prevent the reagent kit 20 from easily tipping over.

[0431] Please refer to the 30th embodiment. Figure 15 and Figure 20 The embodiment of the present application provides a test kit 20, which includes a box body 100. The box body 100 includes multiple pool bodies. The pool body includes a accommodating cavity and a support portion connected to the accommodating cavity. The support portion is a solid structure or a hollow structure. Providing a solid or hollow support portion can increase the relative height of the bottom of the accommodating cavity, which can facilitate the absorption of reagents. The corresponding pipetting device does not need to be inserted too deeply, thereby saving the pre-stored amount of reagents. If it is a solid structure, the center of gravity of the box body 100 can also be moved down, making the overall structure more stable; when it is hollow, the plastic material of the box body 100 can be saved.

[0432] The height of the supporting portion is greater than or equal to the wall thickness of the accommodating cavity. The bottom of the accommodating cavity may be a conical bottom, specifically a conical arc bottom, a triangular pyramid bottom or a polygonal pyramid bottom, and the conical bottom extends into the hollow area of ​​the bottom of the supporting portion; or the bottom of the accommodating cavity is a flat bottom, and the accommodating cavity is connected to the supporting portion through the flat bottom.

[0433] When the supporting portion is a hollow structure, the bottom of the supporting portion is provided with an open end or a closed end.

[0434] The accommodating cavity and the supporting portion are integrally formed or detachably connected.

[0435] When the support portion is a hollow structure, the cross section of the hollow structure is square, circular, polygonal or special-shaped, and the height of the support portion is 0.1 to 0.8 times the height of the pool body.

[0436] The aforementioned cell body can specifically be any cell body such as a reagent cell, a detection cell, a sample dilution cell 112 or an accessory placement cell (101-104).

[0437] The aforementioned detection cell is used for impedance detection or optical detection.

[0438] An embodiment of the present application also provides a sample detection device, which includes the aforementioned test kit 20 and a detection seat 300 that cooperates with the test kit 20. The detection seat 300 is used for sample analysis and detection. The detection seat 300 is provided with a power supply component electrically connected to the front cell 120 electrode 121 and the rear cell electrode 165. The detection seat 300 may also be provided with optical detection components located on opposite sides of the optical detection cell (for example, the first cell body 207).

[0439] The reagent kit 20 provided in this embodiment can facilitate pipetting by a pipetting device by providing pool bottoms of different heights. The pipetting device does not need to be inserted too deeply, thereby saving the pre-stored amount of reagents.

[0440] Please refer to the thirty-first embodiment. Figure 15 and Figure 20 The embodiment of the present application provides a reagent kit 20, which includes a box body 100. The box body 100 is provided with an accessory placement area (101-104), and the accessory placement area is used to place accessories (201-204).

[0441] The lower side of the accessory placement area is provided with a liquid-leakage-proof structure, which can be a bottomed pool. The liquid-leakage-proof structure is integrally formed with the box body 100 or is detachably connected. The accessory placement area is provided with a placement hole, and the accessories (201-204) are placed in the placement hole.

[0442] The aperture of the placement hole is smaller than or equal to the maximum radial dimension of the accessory (201-204), so as to prevent the accessory (201-204) from being completely sunk into the placement hole and to prevent the bottom of the accessory (201-204) from contacting and deforming with the bottom of the pool body when the accessory (201-204) is plugged in.

[0443] The box body 100 is provided with a receiving cavity corresponding to the placement hole, and the accessories (201-204) are received in the receiving cavity. The bottom of the receiving cavity is a sealed structure.

[0444] The accommodating cavity and the box body 100 are integrally formed or detachably connected.

[0445] The inner cavity depth of the accommodating cavity is greater than or equal to the length of the accessory (201-204) extending into the accommodating cavity, thereby preventing the accessory (201-204) from protruding too much from the surface of the box body 100 when placed in the accommodating cavity, and at the same time preventing the bottom of the accessory (201-204) from contacting and deforming with the bottom of the pool body when the accessory (201-204) is plugged in.

[0446] The length of the accessories (201-204) extending into the accommodating cavity is 0.4 to 1 times the length of the accessories.

[0447] The embodiment of the present application further provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 that cooperates with the reagent kit 20, and the detection seat 300 is used for sample analysis and detection.

[0448] When the accessories of the reagent kit 20 provided in this embodiment are removed, the accessories are received by the bottom pool to prevent the residual liquid carried on the surface of the accessories from leaking.

[0449] Please refer to the thirty-second embodiment. Figure 15 and Figure 20 An embodiment of the present application provides a detection cup assembly, which includes a first cell body 207 and a second cell body 208 connected to the first cell body 207 .

[0450] The first cell body 207 and the second cell body 208 are connected via a connector 2071 . The connector 2071 is integrally formed with at least one of the first cell body 207 and the second cell body 208 or is detachably connected thereto.

[0451] The connecting member 2071 is provided with at least one cup holder 2072 , and the cup holder 2072 is detachably connected to the first tank body 207 or the second tank body 208 .

[0452] In one embodiment, the connecting member 2071 is provided with a cup holder 2072 and is integrally connected to the first tank body 207 . The cup holder 2072 is provided with a placement hole, and the shape of the placement hole is the same as or different from the cross-sectional shape of the first tank body 207 .

[0453] In one embodiment, the connecting member 2071 is provided with two cup holders 2072 , and the two cup holders 2072 are respectively provided with a first placement hole and a second placement hole. The shapes of the first placement hole and the second placement hole are the same or different.

[0454] The first cell body 207 is a detection cup, and the second cell body 208 is a reagent cup. During detection, the reagent in the second cell body 208 is added to the first cell body 207 to prepare the sample to be detected and then perform the detection.

[0455] An optical detection window is provided on the first cell body 207 for optical detection, such as optical detection through transmitted light or optical detection through scattered light.

[0456] The cross-sectional shapes of the first cell body 207 and the second cell body 208 are the same or different; or the heights of the first cell body 207 and the second cell body 208 are the same or different. The differentiated settings can achieve a fool-proof effect and facilitate assembly and identification.

[0457] The embodiment of the present application further provides a reagent kit 20, which includes a box body 100 and the aforementioned detection cup assembly. The box body 100 is provided with a mounting portion, and the detection cup assembly is provided with a matching portion, and the mounting portion is connected to the matching portion.

[0458] The mating portion may be a raised portion or a recessed portion provided on the side surface of the detection cup assembly, which is mated with the mounting portion; or the mating portion may be a positioning flange provided on the detection cup assembly, which is mated with the mounting portion, for example, by using a flange structure to be hung with the insertion hole on the box body 100.

[0459] The detection cup assembly provided in this embodiment has a novel structure. Specific detection items can be equipped with corresponding required reagents on the cup holder 2072, which can greatly improve detection efficiency and allow for flexible selection of detection items.

[0460] Please refer to the thirty-third embodiment. Figures 15 to 20 , an embodiment of the present application provides a reagent kit 20 , which includes a box body 100 and an assembly seat 160 .

[0461] The box body 100 includes a fore cell 120 equipped with a fore cell 120 electrode 121; an assembly seat 160 is connected to the box body 100, and the assembly seat 160 is equipped with a rear cell electrode 165, and the fore cell 120 electrode 121 and the rear cell electrode 165 are arranged at intervals.

[0462] In the embodiment of the present application, the axis of the front cell 120 electrode 121 and the axis of the rear cell electrode 165 are roughly in the same straight line. Experimental verification shows that when the axis of the front cell 120 electrode 121 and the rear cell electrode 165 are coaxial, the detection accuracy is relatively high. The front cell 120 electrode 121 and the rear cell electrode 165 are both columnar electrodes, and the manufacturing process and assembly process are relatively simple.

[0463] The assembly seat 160 is provided with an axial drainage cavity 167 , which is connected to the fore cell 120 via a microporous sheet 170 . The fore cell 120 electrode 121 and the rear cell electrode 165 are located on both sides of the microporous sheet 170 .

[0464] The microporous sheet 170 includes a sheet body 171 having micropores 172 that allow cells to pass through one by one. The microporous sheet 170 is mounted on the assembly base 160 or the box body 100, or the microporous sheet 170 and the assembly base 160 or the box body 100 are an integrally formed structure.

[0465] The axis of the electrode 121 of the forecell 120 , the axis of the backcell electrode 165 , and the axis of the microporous sheet 170 are substantially aligned in a straight line.

[0466] Electrode 121 of the forecell 120 protrudes, is flush with, or is recessed in the inner wall of the forecell 120; electrode 165 of the rear cell protrudes, is flush with, or is recessed in the bottom wall of the axial drainage cavity 167. The ends of electrode 121 of the forecell 120 and electrode 165 of the rear cell are not particularly limited. During testing, electrodes 121 of the forecell 120 and electrode 165 of the rear cell make contact with the test fluid. The outer ends of electrodes 121 of the forecell 120 and electrode 165 of the rear cell are used to connect to external power supply components.

[0467] The box body 100 is provided with a mounting cavity 130 , and the assembly seat 160 includes an assembly cylinder 168 , and the mounting cavity 130 and the assembly cylinder 168 are coaxially connected.

[0468] The assembly cylinder 168 and the mounting cavity 130 are snap-fitted, threadedly fitted, interference-fitted, laser-welded, or adhesively fitted.

[0469] The front cell 120 electrode 121 is disposed on the box body 100 and protrudes, is flush with, or is recessed from the outer side of the box body 100 . The rear cell electrode 165 is disposed on the assembly seat 160 and protrudes, is flush with, or is recessed from the outer side of the assembly seat 160 .

[0470] The embodiment of the present application further provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 that cooperates with the reagent kit 20, and the detection seat 300 is used for sample analysis and detection.

[0471] Please refer to the thirty-fourth embodiment. Figure 15 and Figure 20 , an embodiment of the present application provides a reagent kit 20 , the reagent kit 20 includes a box body 100 and a gripping portion 150 , and the gripping portion 150 is provided on the box body 100 .

[0472] The grip portion 150 may be disposed near the center of a side edge of the box body 100 , or near the center of an upper surface of the box body 100 .

[0473] There are two gripping portions 150 , which are respectively disposed on two opposite sides of the box body 100 .

[0474] The gripping portion 150 protrudes from the upper surface or side surface of the box body 100. The outer surface of the gripping portion 150 is provided with a snap protrusion 151 or a snap recess.

[0475] An anti-slip portion 152 is provided on the top or outer side of the grip portion 150 .

[0476] The gripping portion 150 is an elastic member. A recessed portion 154 is provided on the side of the box body 100 . The gripping portion 150 is cooperatively connected with the recessed portion 154 .

[0477] The gripping portion 150 and the recessed portion 154 are integrally connected or detachably connected.

[0478] The gripping portion 150 is slidably connected to the recessed portion 154 so that the gripping portion 150 and the box body 100 can move relative to each other. The gripping portion 150 can be extended and retracted relative to the box body 100 for easy storage without taking up space. The gripping portion 150 can be pulled out when needed.

[0479] The embodiment of the present application further provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 that cooperates with the reagent kit 20 , and the gripping portion 150 is cooperatively connected to the detection seat 300 .

[0480] Please refer to the thirty-fifth embodiment. Figure 15 and Figure 20 , an embodiment of the present application provides a reagent kit 20 , which includes a box body 100 and a blocking portion 153 .

[0481] The box body 100 includes at least one well position (eg, a sample holding well 105 ); the well position is used to place a test tube (eg, a sample tube 205 ).

[0482] The blocking portion 153 is provided at one side of the pool position and is used to hook the test tube cap (not shown) of the test tube to prevent the opened test tube cap from returning to the open end of the test tube.

[0483] In a specific embodiment, the blocking portion 153 can be protruding, flush, or recessed on the upper surface or side surface of the box body 100 .

[0484] In one embodiment, the blocking portion 153 includes a connector connected to the box body 100 and a stopper extending from the connector. A hook portion extending toward the box body 100 is provided at the end of the stopper.

[0485] In another embodiment, the side surface of the box body 100 protrudes outward to form the blocking portion 153; or the blocking portion 153 is a groove set on the upper surface of the box body 100; or the upper surface of the box body 100 is provided with a recessed portion, and the inner wall of the recessed portion forms the blocking portion 153. After the test tube cap of the test tube is opened, it can be inserted into the recessed portion, and the size of the recessed portion can correspond to the size of the test tube cap to achieve a relatively tight fit.

[0486] The blocking portion 153 is integrally formed with the box body 100 or is detachably connected thereto. Specifically, the blocking portion 153 is snap-fitted, plug-fitted, threaded, or detachably connected thereto via screws or pins.

[0487] In other embodiments, the blocking portion 153 is also slidably connected to the box body 100 .

[0488] In the embodiment of the present application, the reservoir (eg, the sample containing reservoir 105 ) is disposed close to the edge of the box body 100 , and the blocking portion 153 is disposed at the edge of the box body 100 .

[0489] The above is only an implementation method of the present application and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the description and drawings of this application, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A POCT blood cell analyzer, characterized in that: The POCT blood cell analyzer includes: case; A detection seat, which can be extended into or out of the housing, is used to receive a reagent kit having an impedance detection pool. The detection seat is provided with a power supply for impedance detection. The reagent kit has a mounting head accommodating pool and a plurality of functional pools. The mounting head accommodating pool is used to install the mounting head. A pipette is provided in the shell and located above the detection seat. The pipette is used to load the installation head to perform corresponding operations. When the installation head needs to be unloaded, the pipette moves to a preset position outside the installation head accommodating pool to perform the installation head unloading operation, wherein the preset position is a functional pool of the test kit, and the caliber of the functional pool at the preset position is larger than the caliber of the installation head accommodating pool. The functional pool includes a diluent pool, a WBC detection pool, and an RBC detection pool.

2. A POCT blood cell analyzer, characterized in that: The POCT blood cell analyzer includes: case; A detection seat, which can be extended into or out of the housing, is used to receive a reagent kit having an impedance detection pool. The detection seat is provided with a power supply for impedance detection. The reagent kit has a mounting head accommodating pool and a plurality of functional pools. The mounting head accommodating pool is used to install the mounting head. A pipette, disposed in the housing and located above the detection seat, the pipette being used to load the mounting head to perform corresponding operations; A detector is used to detect whether the mounting head is loaded on the pipette when the POCT hematology analyzer needs to unload the mounting head. If it is detected that the mounting head is loaded on the pipette, the pipette moves to a preset position outside the mounting head accommodating pool to perform the mounting head unloading operation, wherein the preset position is a functional pool of the reagent kit, and the caliber of the functional pool at the preset position is larger than the caliber of the mounting head accommodating pool, and the functional pool includes a diluent pool, a WBC detection pool, and an RBC detection pool.

3. A POCT blood cell analyzer, characterized in that: The POCT blood cell analyzer is used to receive the test kit, and the POCT blood cell analyzer includes: A detection seat is used to receive the reagent kit provided with an impedance detection pool, the detection seat is provided with a power supply for impedance detection, the reagent kit has a mounting head accommodating pool and a plurality of functional pools, the mounting head accommodating pool is used to install the mounting head; A pipette is provided above the detection seat, and is used to load the installation head to perform corresponding operations on the test kit. When the installation head needs to be unloaded, the pipette moves to the installation head accommodating pool or a preset position outside the installation head accommodating pool to perform the installation head unloading operation, wherein the preset position is a functional pool of the test kit, and the caliber of the functional pool at the preset position is larger than the caliber of the installation head accommodating pool, and the functional pool includes a diluent pool, a WBC detection pool, and an RBC detection pool.

4. The POCT blood cell analyzer according to claim 3, characterized in that: The POCT blood cell analyzer also includes a detector, which is used to detect whether the mounting head is loaded on the pipette when the POCT blood cell analyzer needs to unload the mounting head. If it is detected that the mounting head is loaded on the pipette, the pipette unloads the mounting head to a preset position outside the mounting head accommodating pool.

5. The POCT blood cell analyzer according to claim 3, characterized in that: The pipette comprises an air guide tube and an outer sleeve, and the mounting head is unloaded when the outer sleeve moves axially relative to the air guide tube.

6. A method for using a POCT blood cell analyzer, characterized in that: Based on the POCT blood cell analyzer according to claim 1, the method of use comprises: The pipette of the POCT hematology analyzer moves to the mounting head accommodating pool to load the mounting head; When the mounting head needs to be unloaded, the pipette of the POCT hematology analyzer moves to a preset position outside the mounting head accommodating pool to perform the mounting head unloading operation.

7. A method for using a POCT blood cell analyzer, characterized in that: Based on the POCT blood cell analyzer according to claim 2, the method of use comprises: The pipette of the POCT hematology analyzer moves to the mounting head accommodating pool to load the mounting head; When the mounting head needs to be unloaded, detecting whether the pipette of the POCT hematology analyzer is loaded with the mounting head; If it is detected that the mounting head is loaded on the pipette, the pipette moves to a preset position outside the mounting head accommodating pool to perform a mounting head unloading operation.

8. A method for using a POCT blood cell analyzer, characterized in that Based on the POCT blood cell analyzer according to any one of claims 3 to 5, the method of use comprises: The pipette of the POCT hematology analyzer moves to the mounting head accommodating pool to load the mounting head; When the installation head needs to be unloaded, detecting whether the POCT blood cell analyzer is equipped with a reagent kit; Detecting whether a mounting head is loaded on the pipette of the POCT hematology analyzer; If it is detected that the POCT blood cell analyzer is equipped with the reagent kit and the pipette is loaded with the mounting head, the pipette is controlled to move to the mounting head accommodating pool or a preset position outside the mounting head accommodating pool to perform a mounting head unloading operation.

9. The method for using the POCT blood cell analyzer according to claim 8, characterized in that: The preset position corresponds to the interior of the detection seat or to the exterior of the detection seat.

10. The method for using the POCT blood cell analyzer according to claim 8, characterized in that: The outer sleeve of the pipette moves axially relative to the air guide tube of the pipette to unload the mounting head.

11. The method for using the POCT blood cell analyzer according to claim 8, characterized in that: The pipette unloads the mounting head through a fixed blocking member or a servo-disposed retractable blocking member, wherein the blocking member is a retractable motor, a vertically or horizontally disposed electromagnet, or a U-shaped baffle.

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